Abstract
While the presence of pinworm eggs in archaeological samples has been reported by many researchers in the New World, those have been detected very scarcely in the Old World, especially in East Asian countries. In fact, many parasite species were recovered from the archeological remains in Korea, eggs of Enterobius vermicularis had not been found. Recently, a female mummy buried in the 17th century was discovered in the Joseon tomb from Dangjin-gun, Chungcheongnam-do, Korea. After rehydration process for 12 days, investigations were carried on the luminal surface of the colon. From them, 3 eggs of E. vermicularis were recovered. They were elliptical, transparent with a thin egg shell, 50.3±5.2 µm (length) and 28.2±3.9 µm (width) in size. This is the first discovery of E. vermicularis eggs in East Asia.
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Key words: Enterobius vermicularis, mummy, egg, 17th century
Paleoparasitologists in Korea have examined ancient human samples from archeological fields in Korea, studying parasite infections prevalent in the past. Parasite eggs or larvae remained in mummies of Joseon Dynasty (1392-1910 AD) or in soil sediments from archeological sites became invaluable resources for studying ancient parasites that had infected Korean population. We have reported various ancient parasite species, including
Ascaris lumbricoides,
Trichuris trichiura,
Metagonimus yokogawai,
Clonorchis sinensis,
Paragonimus westermani,
Gymnophalloides seoi,
Strongyloides stercoralis, and
Trichostrongylus spp. [
1-
3]. However, even though some parasite eggs (e.g. pinworms and hookworms) were known to be one of the most common parasite species infecting Korean population, we still did not observe them in ancient samples.
In particular, the pinworm,
Enterobius vermicularis, is a helminth infecting nearly a billion people worldwide in all socioeconomic levels [
4,
5]. The parasite can be transmitted from host to host without stages in soil or intermediary hosts. In case of New World countries, the presence of pinworm eggs in archeological samples has been reported by many researchers. Briefly,
E. vermicularis infection was proven among parasitological samples from ancient people in North America. The parasite eggs were found in a 10,000-year-old human coprolite from Utah, USA, one of the oldest human coprolites ever found and in the mummies' coprolites from several North American archaeological sites [
6].
E. vermicularis infection was also identified in ancient Andean peoples. The pinworm eggs were observed in ancient coprolites from Chile, Peru, and Argentina [
6].
However, interestingly enough,
E. vermicularis has been detected very scarcely in the Old World [
5,
7]. Up to the present, there were only 2 reports of
E. vermicularis eggs in Roman latrines [
8] and in an Egyptian mummy [
9]. Especially in case of East Asian countries, there were no reports on the presence of
E. vermicularis in archeologically obtained samples. In this regard, the current case, a new Joseon mummy discovered in an archeological site of Korea, should be significant to concerned researchers in Korea. We found well-preserved
E. vermiculars eggs, at this moment, showing the first-ever evidence for the preservation of ancient pinworm eggs in archeologically obtained samples from East Asian countries.
On August 2008, a female mummy was discovered in the Joseon tomb from Dangjin-gun, Chungcheongnam-do, Korea (
Fig. 1A). Based on the tree-ring test, the tomb was confirmed to be constructed in 1630s AD [
10]. After the mummy was moved to our laboratory, the researchers wearing sterilized gowns, gloves, head caps, and masks removed the clothes wrapped around the dead body (
Fig. 1B). Anthropologists dissected the abdominal cavity in which mummified intestines were preserved very well (
Fig. 1C). We collected parasitological samples from luminal surfaces of ascending, transverse, and descending colons.
Obtained samples were rehydrated in 0.5% trisodium phosphate solution for 12 days with occasional shaking [
11]. They were then filtered through multiple-layered gauze, after which, were precipitated for a day. After the upper turbid layer was discarded, the precipitates were dissolved again in 0.5% trisodium phosphate solution. The solution was finally dropped onto slides for examination under a light microscope (BH-2, Olympus, Tokyo, Japan). The sizes of the parasite eggs were measured.
In the light microscopic observation, we observed 2 eggs of
Paragonimus westermani, 8 of
Ascaris lumbricoides, and 3 of
Enterobius vermicularis. As for
E. vermicularis eggs, the average size was 50.3±5.2 µm (length) by 28.2±3.9 µm (width).
E. vermicularis eggs showed typical characteristics; small, elliptical, and transparent with a thin eggshell (
Fig. 2). The average size of
A. lumbricoides eggs was 66.0±5.7×50.9±6.8 µm, and those of
P. westermani were 75.5±1.7×48.3±1.8 µm in the state of operculum-missing.
Besides the common paleoparasitological interest on whether specific species of parasites infected people buried in ancient tombs, studies on
E. vermicularis have special meaning to concerned researchers. Briefly, it is considered that the human-
E. vermicularis relationship started in pre-hominid times, having evolved in Africa, and then dispersed to other continents by prehistoric human migrations [
6,
12,
13]. Interestingly enough, many paleoparasitologists believed that ancient pinworms crossed the Bering Land Bridge with its human host during their first migration to the Americas even if transpacific routes have also been postulated by some paleoparasitologists [
6].
However, though there were many reports on the presence of
E. vermicularis in the samples from archeological fields, observation of parasite eggs was very rarely reported from East Asian countries in spite of its endemicity. Of course, the preservation status varies in different species of parasite eggs. Relatively poorer preservation status of
E. vermicularis eggs was well noted for archeologically obtained fecal remains [
6,
14]. However, considering that
E. vermicularis infection reached very high prevalence as shown in some prehistoric coprolites containing ancient eggs [
5,
15], the absence of reports on the presence of
E. vermicularis eggs from East Asian countries looks very strange.
For the past several years, though we have tried to locate the ancient E. vermicularis eggs in many samples from archeological fields, we could not find any. In this regard, the current report on the ancient E. vermicularis eggs from several-hundred-year old Joseon mummy could be a contribution to future studies over the issue. In addition, we showed the possibility that ancient E. vermicularis eggs could also be remained in the samples from archeological sites in East Asia for the first time. Though we are not sure why ancient E. vermicularis eggs in Old World countries were not preserved as perfectly as seen in New World counterparts, more detailed and cautious examinations on the archeological samples could provide invaluable information on the migration of ancient pinworms crossing the Bering Land Bridge with its human host.
ACKNOWLEDGEMENTS
This study was funded by the National Research Institute of Cultural Heritage, 2011, Korea (NRICH-1107-B10F-1).
References
- 1. Seo M, Guk SM, Kim J, Chai JY, Bok GD, Park SS, Oh CS, Kim MJ, Yil YS, Shin MH, Kang IU, Shin DH. Paleoparasitological report on the stool from a Medieval child mummy in Yangju, Korea. J Parasitol 2007;93:589-592.
- 2. Seo M, Shin DH, Guk SM, Oh CS, Lee EJ, Shin MH, Kim MJ, Lee SD, Kim YS, Yi YS, Spigelman M, Chai JY. Gymnophalloides seoi eggs from the stool of a 17th century female mummy found in Hadong, Republic of Korea. J Parasitol 2008;94:467-472.
- 3. Shin DH, Chai JY, Park EA, Lee W, Lee H, Lee JS, Choi YM, Koh BJ, Park JB, Oh CS, Bok GD, Kim WL, Lee E, Lee EJ, Seo M. Finding ancient parasite larvae in a sample from a male living in late 17th century Korea. J Parasitol 2009;95:768-771.
- 4. Lukes J, Horák A, Scholz T. Helminth genome projects: All or nothing. Trends Parasitol 2005;21:265-266.
- 5. Iñiguez AM, Reinhard KJ, Araújo A, Ferreira LF, Vicente AC. Enterobius vermicularis: Ancient DNA from North and South American human coprolites. Mem Inst Oswaldo Cruz 2003;98:67-69.
- 6. Gonçalves ML, Araújo A, Ferreira LF. Human intestinal parasites in the past: New findings and a review. Mem Inst Oswaldo Cruz 2003;98:103-118.
- 7. Bouchet F, Guidon N, Dittmar K, Harter S, Ferreira LF, Chaves SM, Reinhard K, Araújo A. Parasite remains in archaeological sites. Mem Inst Oswaldo Cruz 2003;98:47-52.
- 8. Herrmann B. Parasitologisch-epidemiologische auswertungen mittelalterlicher kloaken. Z Archaol Mittelalters 1985;13:131-161.
- 9. Horne PDJ. First evidence of enterobiasis in ancient Egypt. J Parasitol 2002;88:1019-1021.
- 10. Chungnam Institute of History and Culture. Dangjin Uduri Yujok (II). 2010, Gongju. pp 553-562.
- 11. Van Cleave HJ, Ross JA. A method for reclaiming dried zoological specimens. Science 1947;105:318.
- 12. Hugot JP, Reinhard KJ, Gardner SL, Morand S. Human enterobiasis in evolution: Origin, specificity and transmission. Parasite 1999;6:201-208.
- 13. Iñiguez AM, Reinhard K, Carvalho Gonçalves ML, Ferreira LF, Araújo A, Paulo Vicente AC. SL1 RNA gene recovery from Enterobius vermicularis ancient DNA in pre-Columbian human coprolites. Int J Parasitol 2006;36:1419-1425.
- 14. Reinhard KJ, Hevly RH, Anderson GA. Helminth remains from prehistoric Indian coprolites on the Colorado Plateau. J Parasitol 1987;73:630-639.
- 15. Reinhard KJ. In Cockburn A, Cockburn E, Reyman TA eds, Mummy studies and archaeoparasitology. Mummies, Disease and Ancient Cultures. 1998, Cambridge, UK. Cambridge University Press. pp 377-380.
Fig. 1(A) The location of Dangjin-gun (red dot), Chungcheongnam-do, Korea, where the female mummy was discovered. (B) The female mummy, presumably buried in 1630s AD. (C) The mummified intestines were dissected from the abdominal cavity.
Fig. 2Microscopic examination of 3 Enterobius eggs recovered from the mummy. Bar=20 µm.
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