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Original Article

Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts

Hossein Mahmoudvand1,2, Ebrahim Saedi Dezaki2, Farnaz Kheirandish3, Behrouz Ezatpour4, Sareh Jahanbakhsh1,2, Majid Fasihi Harandi5
The Korean Journal of Parasitology 2014;52(6):653-659.
Published online: December 23, 2014

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Corresponding author (majid.fasihi@gmail.com)
• Received: July 11, 2014   • Revised: August 9, 2014   • Accepted: August 26, 2014

© 2014, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
Korean J Parasitol. 2014;52(6):653-659.   Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.653
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Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
Korean J Parasitol. 2014;52(6):653-659.   Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.653
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Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
Image Image
Fig. 1 Live protoscolices of Echinococcus granulosus after exposure to 0.1% eosin.
Fig. 2 Dead protoscolices of Echinococcus granulosus after exposure to N. sativa and thymoquinone with 0.1% eosin.

Fig. 1

Fig. 2

Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
No Compound Percentage
1 Camphene 0.06
2 t-Anethole 2.3
3 β-Pinene 0.03
4 α-Pinene 0.04
5 γ-Terpinene 0.4
6 β-Myrcene 0.05
7 α-Terpinene 0.01
8 Limonene 1.7
9 1,8-Cineole 0.16
10 Sabinene 1.3
11 ρ-Cymene 14.1
12 α-Terpinolene 0.01
13 ρ-Cymene-8-ol 0.42
14 Carvacrol 10.3
15 Longipinene 0.4
16 Camphor 1.5
17 Linaloolcis 0.21
18 Sabinenehydrate 0.2
19 Longifolene 6.1
20 Bornylacetate 0.53
21 Thymol 1.2
22 4-Terpineol 5.1
23 Borneol 0.14
24 Carvone 0.08
25 Thymoquinone 42.4
26 2-Tridecanone 0.8
27 Thujone 1.5
28 ρ-Anisaldehyde 0.1
29 2-Undecanone 0.21
30 Unknown peak 3.1
Total 96.6
Concentration (mg/ml) Exposure time (min) Mean of mortality rate (%)
10 5 82.3
10 100
20 100
30 100
60 100
5 21.3
1 10 67.6
20 100
30 100
60 100
5 8.3
0.1 10 21.6
20 32.3
30 55.6
60 76.6
5 4.6
0.01 10 14.6
20 23.3
30 36.3
60 52.6
5 0
Normal saline+Tween 20 10 1.3
20 2.6
30 4.3
60 6.6
20% hypertonic saline 5 81.3
10 100
20 100
30 100
60 100
Concentration (mg/ml) Exposure time (min) Mean of mortality rate (%)
1 5 58.6
10 100
20 100
30 100
60 100
5 18.3
0.5 10 38.6
20 100
30 100
60 100
5 7.3
0.25 10 17.6
20 35.6
30 96.3
60 100
5 3.3
0.125 10 19.6
20 21.3
30 39.6
60 87.3
5 0
Normal saline+Tween 20 10 1.3
20 2.6
30 4.3
60 6.6
20% hypertonic saline 5 81.3
10 100
20 100
30 100
60 100
Table 1. Essential oil composition of N. sativa seeds identified by GC-MS
Table 2. Scolicidal effects of essential oil of N. sativa against protoscoleces of hydatid cyst at various concentrations following various exposure times
Table 3. Scolicidal effects of thymoquinone against protoscoleces of hydatid cyst at various concentrations following various exposure times

Table 1.

Table 2.

Table 3.

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