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Original Article

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions

Shuai Wang1, Aiping Cao1, Xun Li2, Qunli Zhao1, Yuan Liu1, Hua Cong1, Shenyi He1, Huaiyu Zhou1,*
The Korean Journal of Parasitology 2015;53(3):253-258.
Published online: June 30, 2015

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*Corresponding author (zhouhy@sdu.edu.cn)
• Received: January 14, 2015   • Revised: May 3, 2015   • Accepted: May 3, 2015

© 2015, Korean Society for Parasitology and Tropical Medicine

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  • Superoxide Dismutases in Immune Regulation and Infectious Diseases
    Tong Liu, Jiajin Shang, Qijun Chen
    Antioxidants.2025; 14(7): 809.     CrossRef
  • Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
    Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
    BMC Infectious Diseases.2017;[Epub]     CrossRef

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Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Korean J Parasitol. 2015;53(3):253-258.   Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.253
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Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Korean J Parasitol. 2015;53(3):253-258.   Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.253
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Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Image Image Image
Fig. 1. Analysis of PCR products of SOD gene from 10 examined T. gondii strains using electrophoresis. Lane M; DNA size marker 10,000. Lanes 1-10; T. gondii type I (GT1, RH), type II (PTG, PRU), type III (CTG) strains and other strains (TgCgCa1, MAS ,TgCatBr5 ,TgCatBr64, and TgToucan), respectively.
Fig. 2. PCR-RFLP analysis of SOD genes using restriction endonucleases EcoR I (upper) and Xba I (bottom). Lane M; DNA size marker 2,000. Lanes 1-10; T. gondii type I (GT1, RH), type II (PTG, PRU), type III (CTG) strains and other strains (TgCgCa1, MAS, TgCatBr5, TgCatBr64, and TgToucan), respectively.
Fig. 3. Phylogenetic analyses of 10 Toxoplasma gondii strains based on analysis of the SOD gene sequences. The tree was built by Bayesian inference (BI) analysis. The numbers along branches indicate bootstrap values resulting from BI analysis.

Fig. 1.

Fig. 2.

Fig. 3.

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions
Strain Host Geographical origin Genotypea
RH Human  France Reference, Type I, ToxoDB #10
GT1 Goat  United States Reference, Type I, ToxoDB #10
PTG Sheep  United States Reference, Type II, ToxoDB #1
Prugniaud (PRU) Human  France Reference, Type II, ToxoDB #1
CTG Cat  United States Reference, Type III, ToxoDB #2
TgCgCa1 Cougar  Canada Reference, ToxoDB #66
MAS Human  France Reference, ToxoDB #17
TgCatBr5 Cat  Brazil Reference ToxoDB #19
TgCatBr64 Cat  Brazil Reference ToxoDB #111
TgToucan (TgRsCr1) Toucan  Costa Rica Reference, ToxoDB #52
RH CTG GT1 MAS PRU PTG TgCatBr5 TgCatBr64 TgCgCa1 TgToucan
1403 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡
1437 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡
1480 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡
1501 G ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ - ﹡
1503 G ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1504 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡
1518 A ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1519 C ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡
1520 T ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡ ﹡
1521 G ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1532 C ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1535 C ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡ ﹡
1545 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ G ﹡
1547 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡
1549 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ G ﹡
1555 C ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1556 A ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1557 T ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡ ﹡
1558 C ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡
1566 A ﹡ ﹡ ﹡ ﹡ ﹡ G ﹡ ﹡ ﹡
1567 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡
1568 C ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡
1574 G ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡ ﹡
1575 G ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡
1584 T ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ ﹡
1585 C ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡
1586 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡
1587 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ G ﹡ ﹡
1590 G ﹡ ﹡ ﹡ ﹡ A A ﹡ ﹡ ﹡
1599 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡
1601 C ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡
1614 G ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡ ﹡
1616 T ﹡ ﹡ ﹡ ﹡ G ﹡ ﹡ ﹡ ﹡
1618 G ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡ ﹡ ﹡
1620 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡
1628 T ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡ ﹡
1635 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ C
1637 C ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡ ﹡
1668 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡
1669 C ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡
1679 G ﹡ ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡
1685 C ﹡ ﹡ ﹡ ﹡ A ﹡ ﹡ ﹡ ﹡
1793 C ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡ ﹡
RH CTG GT1 MAS PRU PTG TgCatBr5 TgCatBr64 TgCgCa1 TgToucan
66 N ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ Y ﹡ ﹡
80 S ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡ ﹡
87 G ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ - ﹡
88 G ﹡ ﹡ ﹡ ﹡ ﹡ C ﹡ V ﹡
93 T ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ S ﹡ ﹡
94 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ W ﹡ ﹡
102 K ﹡ ﹡ ﹡ ﹡ ﹡ N ﹡ E ﹡
103 E ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ G ﹡
105 T ﹡ ﹡ ﹡ ﹡ ﹡ I ﹡ ﹡ ﹡
106 S ﹡ ﹡ ﹡ ﹡ ﹡ F ﹡ ﹡ ﹡
109 N ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ I ﹡
112 D ﹡ ﹡ ﹡ ﹡ ﹡ N ﹡ ﹡ ﹡
115 S ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ P ﹡ ﹡
116 K ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ E ﹡ ﹡
117 V ﹡ ﹡ ﹡ ﹡ I I ﹡ ﹡ ﹡
120 G ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ S ﹡
125 G ﹡ ﹡ ﹡ ﹡ S ﹡ ﹡ ﹡ ﹡
126 W ﹡ ﹡ ﹡ ﹡ F ﹡ ﹡ ﹡ ﹡
127 A ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ ﹡ T ﹡
129 L ﹡ ﹡ ﹡ ﹡ H ﹡ ﹡ ﹡ ﹡
132 D ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡ H
143 T ﹡ ﹡ ﹡ ﹡ ﹡ N ﹡ - ﹡
148 T ﹡ ﹡ ﹡ ﹡ N ﹡ ﹡ ﹡ ﹡
151 T ﹡ ﹡ ﹡ ﹡ ﹡ - ﹡ ﹡ ﹡
Table 1. Toxoplasma gondii strains subjected to SOD gene sequence analysis

Based on genotyping results of Su et al. [17].

Table 2. Nucleotide polymorphisms of the SOD gene coding region within Toxoplasma gondii strains

Numbers in the left column indicate positions of variable nucleotides. Asterisks (﹡) indicate identical nucleotides related to the sequence of RH (first column). Dashes (-) indicate deletions.

Table 3. Amino acid changes of the SOD gene coding region among ten Toxoplasma gondii strains

Numbers in the left column indicate positions of variable amino acids. Asterisks (﹡) indicate identical amino acid related to the sequence of RH (first column). Dashes (-) indicate deletions.

Table 1.

Table 2.

Table 3.

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