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Original Article

Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea

Hyeong-Kyu Jeon1, Hansol Park1, Dongmin Lee1, Seongjun Choe1, Kyu-Heon Kim2, Sun Huh3, Woon-Mok Sohn4, Jong-Yil Chai5, Keeseon S. Eom1,*
The Korean Journal of Parasitology 2015;53(3):299-305.
Published online: June 30, 2015

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*Corresponding author (kseom@chungbuk.ac.kr)
• Received: March 10, 2015   • Revised: May 12, 2015   • Accepted: May 18, 2015

© 2015, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea
Korean J Parasitol. 2015;53(3):299-305.   Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.299
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Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea
Korean J Parasitol. 2015;53(3):299-305.   Published online June 30, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.3.299
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Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea
Image Image
Fig. 1. Gravid proglottids of Spirometra erinaceieuropaei and Spirometra decipiens. (A, B) Whole mounted specimens of proglottids showing the uterus and cirrus sac (×40), (C, D) Longitudinal section of a gravid proglottids showing the cirrus sac (CS), seminal vesicle (SV), and uterus (U) (H&E stain).
Fig. 2. A phylogenetic tree of spirometrid tapeworms based on partial cox1 sequences inferred from neighbor-joining analysis. Numbers on branches indicate the bootstrap supporting values based on 1,000 replicates and bootstrap values by the maximum parsimony, maximum likelihood, and neighbor-joining methods are shown from left to right. The partial cox1 sequences of S. erinaceieuropaei and S. decipiens were divided into 2 clades, which differed by 29-32 nucleotides from each other. There were 390 bp corresponding to positions 732-1,122 bp of the cox1 gene.

Fig. 1.

Fig. 2.

Human Infections with Spirometra decipiens Plerocercoids Identified by Morphologic and Genetic Analyses in Korea
Code Locality Sex/Age Year Molecular identification Genbanka
G1249 Cheongju (Chungbuk) M/49 2001 S. erinaceieuropaei
G1254 Goesan (Chungbuk) M/60 1996 ″
G1286 Chungbuk M/50 1997 ″
G1295 Cheongwon (Chungbuk) F/78 1998 ″
G1300 Jeju - 2002 S. decipiens
G1301 Jeju - 2002 ″
G1302 Jeju - 2002 ″
G1326 Sancheong (Gyoungnam) M/64 2002 S. erinaceieuropaei
G1327 Sancheong (Gyoungnam) M/64 2002 ″
G1328 Sancheong (Gyoungnam) M/64 2002 S. decipiens
G1343 Seoul - 1979 ″
G1344 - F/59 - S. erinaceieuropaei
G1345 Seoul - - ″
G1346 Seoul - - ″
G1347 Seoul - - ″
G1348 - F/51 1987 ″
G1353 Seoul F/66 2003 ″
G1354 Seoul M/79 2003 ″
G1356 Cheongju (Chungbuk) M/37 2000 ″
G1357 - M/41 1999 ″
G1393 Seoul F/42 2003 ″
G1394 Seoul - 2003 ″
G1395 Seoul F/49 2003 ″
G1396 Seoul - 2003 ″
G1398 Cheonan (Chungnam) F/44 2003 ″
G1401 Seoul F/59 2003 ″
G1402 Cheonan (Chungnam) F/49 2003 ″
G1404 Busan - 1990 ″
G1405 Busan - 1990 ″
G1406 Busan F/23 1990 S. decipiens
G1407 Busan F/47 1994 ″
G1409 Busan M/65 1995 S. erinaceieuropaei
G1410 Busan M/65 1995 ″
G1411 Busan F/55 2001 ″
G1412 Busan M 2003 S. decipiens
G1413 Busan M/71 2003 ″
G1476 Busan M/71 2003 S. erinaceieuropaei
G1530 Busan M/72 2003 ″
G1574 Gangwon F/68 2002 S. decipiens
G1576 Gangwon F/57 2005 S. erinaceieuropaei
G1578 Jinju (Gyeongnam) M/42 2005 ″
G1622b Chungbuk S. decipiens KJ599679
G1625 Seoul F/52 2006 ″
G1628 Gangwon F/57 2002 ″
G1657 Cheongju (Chungbuk) M/59 2008 ″
G1681 Cheongju (Chungbuk) F/46 2008 S. erinaceieuropaei
G1718 Cheongju (Chungbuk) F/42 1994 ″
G1719 Chungbuk M/45 2008 S. decipiens
G1720 Chungbuk F/67 2009 S. erinaceieuropaei
G1721 Jinju (Gyeongnam) - 2002 S. decipiens
G1724c Cheongju (Chungbuk) M/58 2009 S. erinaceieuropaei KJ599680
Organs Morphologic features S. erinaceieuropaei
 S. decipiens
 Others
Size (mm) Size (mm)
Scolex Spatulate 0.2 0.5 Diameter
1 1.5 Length
Gravid proglottids Trapezoid 3.1-4.2 6.8-10.8 Width
2.1-3.2 1.1-2.0 Length
Uterus Coiling 5-7 4-4½ Loops
Eggs Operculate 0.057-0.061 × 0.033-0.036 0.056-0.060 × 0.034-0.036 Length width
Table 1. Spargana specimens analyzed in this study (1979-2009)

Accession numbers in GenBank, National Center for Biotechnology Information, USA.

The sparganum was obtained from a snake and a Spirometra decipiens adult was recovered after experimental infection of a cat.

The sparganum was obtained from a patient (molecular study was done cutting a small part of this worm) and a S. erinaceieuropaei adult was recovered from experimental infection of a dog.

Table 2. Morphologic features of Spirometra erinaceieuropaei and S. decipiens

Table 1.

Table 2.

PHD : Parasites, Hosts and Diseases

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