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Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan
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Original Article

Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan

Haroon Ahmed1,2, Ayesha Malik3, Muhammad Arshad3, Irfan Mustafa4, Mobushir Riaz Khan5, Muhammad Sohail Afzal6, Shahzad Ali7, Muhammad Mobeen8, Sami Simsek2,*
The Korean Journal of Parasitology 2016;54(4):439-446.
Published online: August 31, 2016

1Department of Biosciences,COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan

2Department of Parasitology, Faculty of Veterinary, University of Firat, 23119, Elazig, Turkey

3Department of Zoology, University of Sargodha, Pakistan

4Department of Biology, Government Postgraduate College Chishtian, Punjab, Pakistan

5Department of Remote Sensing & GIS, PMAS-University of Arid Agriculture, Rawalpindi, Pakistan

6Department of Chemistry, School of Science, UMT Lahore, Pakistan

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*Corresponding author (ssimsek@firat.edu.tr)
• Received: April 20, 2016   • Revised: June 9, 2016   • Accepted: June 15, 2016

© 2016, Korean Society for Parasitology and Tropical Medicine

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  • Toxoplasmosis is a protozoan disease that is caused by Toxoplasma gondii in livestock and humans. Due to its medical and veterinary importance, it is essential to study the seroprevalence of T. gondii infection among humans and animals in various parts of the world. The major objective of this study was to determine the seroprevalence and spatial distribution of toxoplasmosis in small ruminants (sheep and goats) of north-eastern region, Pakistan. A total of 1,000 animals comprising of sheep (n=470) and goats (n=530) were examined for T. gondii infection by using ELISA. An epidemiological data was collected in the form of questionnaire. A surface has been generated by using method of interpolation in Arc GIS with the help of IDW (inverse distance weight). The results showed higher seroprevalence of T. gondii in goats (42.8%) as compared to sheep (26.2%). The seroprevalence was higher in females as compared to males in all examined ruminants. Similarly, there is a wide variation in the seroprevalence of T. gondii in different breeds of sheep and goats showing higher seroprevalence in Teddy (52.8%) and Damani breed (34.5%) of goat and sheep’s, respectively. The geographical and spatial distribution of T. gondii shows that it is widely distributed in different parts of the north-eastern region of Pakistan. Our results suggest widespread environmental contamination with T. gondii oocysts. It suggests us that small ruminants could be a potentially important source of T. gondii infection if their infected meat is consumed undercooked.
  • Key words: Toxoplasma gondii, ELISA, seroprevalence, sheep, goat, spatial distribution, Pakistan
Livestock production is a vital component of world’s agriculture. Throughout the world, human populations greatly depend on domestic animals for many purposes, especially the meat production, milk, fat, other dairy products, eggs, and fibers like wool or cashmere as well as other purposes [1]. Out of total 79.61 million hectare (MH) geographical area, only 59.28 MH is the reported area of Pakistan; 62.18% of the total geographical area of Pakistan is the rangeland which is 49.5 MH, and most of the small ruminants and camels are supported by this area [2]. There are many epidemics that cause high mortality and morbidity in livestock sector of Pakistan [3]. The economic losses due to these diseases have been estimated to be 79 billion Pakistani rupees which are almost equal to 1 billion US dollars [4]. Toxoplasmosis is one of the most common parasitic diseases in animals and humans that placed the third on the top of world spread. Globally, 30-65% people are infected with toxoplasmosis [5]. It has economic significance to both veterinary and human medicine because Toxoplasma gondii is responsible for birth defects and abortions in intermediate hosts [5,6].
Toxoplasmosis is a widespread zoonosis that is caused by intracellular parasite, T. gondii [7]. Toxoplasmosis ranks high which lead to death in patients with AIDS. In USA, 10% of AIDS patients and up to 30% in Europe are estimated to die from toxoplasmosis [6]. This parasite is transmitted by contaminated meat (sheep, goat, and pig), milk (sheep, goat, and cattle), and chicken eggs [8]. Milk of these animals contains the tachyzoite stage of the parasite [5]. The use of goat milk is elevated in children with allergy to milk of cattle in many rural areas of Pakistan [9]. Humans can accidentally ingest oocysts that cats have excreted in their stool or it can be transmitted to humans by unwashed vegetables or fruits or contaminated water [7].
The prevalence of T. gondii in Pakistan has increased due to lack of modern farming and open-air markets because of the presence of wandering cats around markets [10]. There are few reports on the prevalence of toxoplasmosis in small ruminants in different parts of Punjab, Pakistan. The seroprevalence in sheep’s of southern Punjab was 19.9% [11] and in Pothowar region, it was 18.2% and 14.3% in sheep and goats, respectively [12], while in Rahim Yar Khan region, it was 11.2% and 25.4% in sheep and goats, respectively [13].
The aim of the present study was to determine the serop revalence and spatial distribution of toxoplasmosis in small ruminants (sheep and goats) of north-eastern region of Punjab province, Pakistan.
Study area
A seroepidemiological survey was conducted in different agro-ecological zones of Punjab province, Pakistan which comprises of Bhalwal, Kotmomin, Sahiwal, Shahpur, Silanwali, and Sargodha districts (Fig. 1).
Topography
Sargodha is located in the northeast region of Pakistan. Geographical coordinates of Sargodha is 32.1667˚ N, 72.5000˚ E. It mainly comprises flat, fertile plains, although there are a few small hills on the Sargodha-Faisalabad Road. The River Jhelum flows on the western and northern sides, and the River Chenab lies on the eastern side of the city. The city has a climate of extreme hot and cold. The maximum temperature reaches 50˚C (122˚F) in summer while in winter the minimum temperature recorded is as low as freezing point. Sargodha is largely an agricultural and industrial city. The high temperature during summer creates much heat and heat affects the health of people living in this area. The humidity reaches up to 69% throughout the year. The monthly amount of precipitation has been recorded at around 82 mm, that's 3 inches.The vast fields in the city have allowed the farmers to develop a range of crops and animal breeding programs. The main livestock for the city include goats, sheep, buffaloes, and cattle.
Sample collection
One thousand blood samples comprising of goats (n=530) and sheep (n=470) were collected randomly from different tehsils (Sargodha, Kotmomin, Bhalwal, Shahpur, Sahiwal, and Silanwali) from January 2013 to December 2013 (Figs. 1, 2). Blood samples (5 ml) were taken from jugular veins of sheep and goats. The sera was separated by centrifugation and stored at -20˚C until used. The study was approved from the committee (no. 125).
Serological test (ELISA)
Serum specimens were tested by ELISA to detect anti-Toxoplasma IgG and IgM antibodies using a commercial ELISA Kit (ID Screen Toxoplasmosis Indirect®, ID-VET Company, Montpellier, France) according to manufacturer’s instructions. The results were also interpreted according to the instructions of the manufacturer.
Epidemiological factors
The seroprevalence was determined on the basis of epidem iological factors. The informations (tehsils, age, sex, breed, and species of livestock) were collected in the form of questionnaire.
Statistical analysis
The statistical analyses were done by using SPSS 18 Software for Windows (chi-square test and t-test).
This is the first epidemiological study on T. gondii infection in sheep from north-eastern region as well as in goats from this region of Pakistan by using serological test. Out of 1,000 examined animals, the seroprevalence of T. gondii was higher in goats (42.8%) than sheep (26.2%). While, based on different breed of goats, it was Teddy (52.8%), Beetal (48.5%), Kamori (47.5%), and Phari (18.5%). Whereas, the seroprevalence based on different breeds of sheep was Dmani (34.5%), Kajili (26.3%), and Desi (20.3%) (Table 1).
The impact of different risk factors on the geographical distribution of T. gondii was determined in the present study. Females were more commonly infected as compared to males in both the ruminant spp. Risk factors for infection with T. gondii in sheep having older age were more infected thus predicting that animals have more chances of infection in old age. The statistical analysis showed significant differences in different age groups of sheep and goats (Table 2).
The results showed that there is a significant variation (P<0.05) in the prevalence and geographical distribution of toxoplasmosis in goats and sheep of rural areas in different districts (Fig. 2). The rate of infection in sheep of district Sargodha (48.3%) was higher followed by Silawali (33.6%), Sahiwal (32.9%), Bhalwal (15.9%), and Shahpur (7.8%), while goats of district Sargodha (60.7%) had a higher seroprevalence followed by Sahiwal (43.8%), Bhalwal (44.2%), Silawali (35.8%), and Shahpur (25.7%). The village-wise prevalence was given in Table 3.
There is a wide variation in geographical and spatial dist ribution of toxoplasmosis in rural areas of north-eastern region of Pakistan. There are different risk areas of toxoplasmosis in rural areas/villages of the north-eastern region of Pakistan.
A surface has been generated by using the method of interpolation in Arc GIS with the help of IDW (inverse distance weight). The following map showed the spatial trends among the goats in the study area. The north-western patch of the map, shown in red color, expressed the percentage of seropr evalence of toxoplasmosis from 9-19. The central portion of the map showed the maximum percentage of seroprevalence ranges from 47-57. The south-western extreme of the study area showed the percentage of seroprevalence from 28-38; this value extended inside the districts up to its central tehsil Sargodha but there was discontinuous appearance of higher seroprevalence shown in brown patch ranging from 38-47 (Fig. 3). Similarly, the surface for sheep has been generated by using the method of interpolation in Arc GIS with the help of IDW. The given map shows the spatial trends among the sheep in the study area (Fig. 4).
The present seroepidemiological survey was based on field samples. It reflected the importance of studies on Toxoplasma on regional basis from ruminant spp. in Pakistan.
Toxoplasmosis is a cosmopolitan zoonotic infection caused by a coccidian protozoan T. gondii, which affects a wide range of hosts, including humans, mammals, and birds. It is one of the leading cause of serious economic losses and caprine abortion in sheep and goat industry on worldwide basis [14].
The results of the present study showed that toxoplasmosis is more prevalent in goats than in sheep in north-eastern region of Pakistan. Our findings are consistent with that of Nisar et al. [12] who reported the higher seroprevalence of T. gondii in goats as compared to sheep in northern Punjab (Pakistan). Similar findings were reported by Ahmed et al. [15] and Ramzan et al. [9] that goats are more susceptible to toxoplasmosis as compared to sheep due to their higher activity and movement. It might increase the probability of contact with contaminated sources. These agreements may be attributed to grazing patterns where many sheep flocks grazed daily while goat flocks examined in this survey were housed. Therefore, the possibility of contact with contaminated food and pastures during grazing season was high in sheep flocks. There is a wider variation of distribution of Toxoplasma within Pakistan and across the world. The results showed that toxoplasmosis is widely distributed in sheep and goats of Sargodha, Sahiwal, Bhalwal, Silanwali, and Shaahpur districts. Similar findings were reported from southern parts of the country [9-11], NWFP [16], and in Iran, India, and China [17-19]. This significant variation in the seroprevalence of toxoplasmosis in different areas is due to difference in the climate, hygienic conditions, farm managements, sample size, and serodiagnostic technique [19].
Toxoplasmosis is a zoonotic disease transmitted by direct or indirect contact with infected animals, soiled pasture and corrals, ingesting infected milk, and with semen from male to female animals [20,21]. The overall estimation of seroprevalence of T. gondii in Nile Delta regions was generally higher than Brucella melitensis. This may be attributed to periodical examination of small ruminants by the Veterinary Service Agency to T. gondii infection and slaughtering the infected animals. These screening procedures reduce the spread of toxoplasmosis among small ruminants compared to toxoplasmosis. Toxoplasmosis is endemic at high levels among the large ruminant population of the studied village in the Nile Delta region of Egypt, where the main risk factor for cattle and buffalo sero-positive status is the presence of sheep or goats in the same household [22].
The results showed that Teddy (52.8%) breed as compared to Beetal (48.5%), Kamori (47.5%), and Phari (18.5%) breeds of goats. The results also showed higher seroprevalence in Teddy as compared to other breed which might be due to poor immune system and management. Similar observations were reported by Ahmad et al. [12] in Jattal, Beetal, and teddy breeds of goats. Whereas, seroprevalence based on different breeds of sheep was Dmani (34.5%), Kajili (26.3%), and Desi (20.3%). The results showed higher seroprevalence in Dmani breed as compared to other breed might be due to poor management. Similar observations were reported by Ahmad et al. [12] in Salt, Afghani, and Awasi breeds of sheep.
The results showed that female animals were more infected as compared to males in both the ruminant species. It has been reported that female animals are more susceptible to toxoplasmosis than males in Pakistan [16].
Risk factors for infection with T. gondii in sheep having older age were more infected as compared to young animals thus predicting that animals have more chances of infection at older age. Similar observations were reported by Shah et al. [16]. Increase in the prevalence of the disease in older animals is due to exposure of animals to the risk factors for a longer period of time than younger ones. Similar findings in sheep and goats were reported by Lashari and Tasawar [11] and Tasawar et al. [10]. Our results were in contrary as reported by Ramzan et al. [9]. So, it is concluded from the present study that the seroprevalence of T. gondii infection in goats slaughtered for human consumption was high as compared to goats in north-east region of Pakistan. The study highlighted the potential public health importance of meat of sheep and goats as source of infection for humans. There should be some eradication strategies, and management practices should be implanted to reduce the prevalence rate in other agro-ecological zones of Pakistan.

AUTHORS CONTRIBUTIONS

HA and AM contributed equally in this study. IM designed the study, MA supervised the study, MRK developed the map, MM interpreted the maps, SA and MSF wrote the discussion section. SS critically reviewed and improved the quality of final manuscript.

The authors fully declare any financial or other potential conflict of interest.

Fig. 1.
The sampling sites of goats and sheep in Bhalwal, Kotmomin, Sahiwal, Shahpur, Silanwali, and Sargodha districts. (1) Bhalwal Tehsil is located in the eastern part of Punjab province of Pakistan. (2) Shahpur Tehsil lies on the Jhelum River. (3) Kot Momin is situated almost 40 km away from Sargodha tehsil. (4) Sahiwal tehsil is a subdivision (Tehsil) of Sargodha district. (5) Sargodha Tehsil is an administrative subdivision of Sargodha district.
kjp-54-4-439f1.gif
Fig. 2.
Showing the proportion of different sampling sites with goats and sheep of study area.
kjp-54-4-439f2.gif
Fig. 3.
Spatial trends of surfaces for the seroprevalence of toxoplasmosis in goats of urban areas.
kjp-54-4-439f3.gif
Fig. 4.
Spatial trends of surfaces for the seroprevalence of toxoplasmosis in sheep of urban areas.
kjp-54-4-439f4.gif
Table 1.
The effect of species, sex, and breed on seroprevalence of toxoplasmosis
Table 1.
Host Sex/Breed Examined (n) Infected (n) Seroprevalence (%) Statistical analysis; chi-square (χ²)
Goat Male 150 35 23.3 0.001
Female 380 192 50.5
Total 530 227 42.8
Sheep Male 72 16 22.2 0.003
Female 398 107 26.9
Total 470 123 26.2
Goat Beetal 167 81 48.5 0.021
Teddy 127 67 52.8
Kamori 121 58 48.0
Pahari 115 21 18.3
Total 530 227 42.8
Sheep Kajli 180 48 26.7 0.001
Desi 177 36 20.3
Damani 113 39 34.5
Total 470 123 26.2
Table 2.
The effect of district and age on seroprevalence of toxoplasmosis
Table 2.
Host Factors No. examined No. infected Seroprevalence (%) Statistical analysis; chi-square (χ²)
Goat Bhalwal 86 38 44.2 0.001
Sahiwal 105 46 43.8
Shahpur 74 19 25.7
Silanwali 148 53 35.8
Sargodha 117 71 60.7
Total 530 227 42.8
Sheep Bhalwal 113 18 15.9 0.002
Sahiwal 70 23 32.9
Shahpur 90 7 7.8
Silanwali 137 46 33.6
Sargodha 60 29 48.3
Total 470 123 26.2
Goat ≤ 1.5 yr 193 71 36.8 0.003
1.5-3 yr 122 49 40.2
≥ 3 yr 215 107 49.8
Total 530 227 42.8
Sheep ≤ 1.5 yr 206 27 13.1 0.004
1.5-3 yr 105 33 31.4
≥ 3 yr 159 63 39.6
Total 470 123 26.2
Table 3.
The effect of herds in different districts on seroprevalence of toxoplasmosis
Table 3.
Hosts Districts Village Name Latitude Longitude No. examined No. infected Seroprevalence (%) No. examined No. infected Seroprevalence (%)
Sheep Bhalwal ChabaPurana 32.3382 72.9711 30 4 13.3 113 18 15.9
Liliani 32.208 72.955 25 7 28.0
KotMomin 32.1883 73.0286 30 2 6.6
Chowki Bhagat 32.049 72.9261 28 5 17.9
Sahiwal Farooka 31.8847 72.417 14 6 42.9 70 23 32.9
Sahiba Balochan 31.9403 72.4125 17 3 17.6
Kotla Mirbaz Khan 31.955 72.3748 21 6 28.6
Lakhiwal 31.9858 72.306 18 8 44.4
Shahpur Aaqil Shah 32.2762 72.4558 23 3 13.0 90 7 7.8
Jalpana 32.2617 72.5198 26 1 3.8
Bhakar Bar 32.3122 72.4924 20 5 25.0
Noon Wala 32.2669 72.3961 21 0 0.0
Silanwali Chak No. 99 N.B 31.9546 72.6351 34 16 47.1 137 46 33.6
Hundewali (Shaheen Abad) 31.922 72.6344 30 6 20.0
Chak 127 NB 31.835 72.5302 36 14 38.9
Basti Rasool Abad 31.8185 72.5468 37 10 27.0
Sargodha Chak 104 SB 31.9995 72.6961 21 4 19.0 60 29 48.3
Chak 36 NB 32.1261 72.7297 12 8 66.7
Jhal Chakian 32.1476 72.6417 10 4 40.0
Chak No. 90 NB 32.038 72.596 17 13 76.5
Goats Bhalwal Phularwan 32.3535 73.0141 22 9 40.9 86 38 44.2
Deowal 32.2151 72.9093 20 5 25.0
Chak 11 SB 32.159 72.9953 28 11 39.3
Mangowal 32.2151 72.8155 15 13 86.7
Sahiwal AzmatWala 31.8946 72.4045 25 9 36.0 105 46 43.8
Fateh Wala 31.9254 72.4356 29 17 58.6
Tibba 31.9606 72.3929 31 4 12.9
Deenpur 31.9683 72.2845 20 16 80.0
Shahpur Shahpur Sadar 32.2673 72.4762 16 2 12.5 74 19 25.7
Jalpana 32.2667 72.517 13 7 53.8
Rehmam Abad 32.3116 72.5332 19 1 5.3
Ichhar 32.2411 72.4218 26 9 34.6
Silanwali Chak No. 117 N.B 31.9358 72.6485 34 16 47.1 148 53 35.8
Chak No. 118 SB 31.9245 72.6469 37 3 8.1
Chak 126 NB 31.8483 72.5362 31 18 58.1
Islam Nagar 31.8208 72.5445 46 16 34.8
Sargodha Awan Abad 32.0011 72.719 24 11 45.8 117 71 60.7
Chak 33 NB 32.1237 72.7504 23 19 82.6
LoddyWala 32.1433 72.6611 33 9 27.3
Chak No. 95 NB 32.0491 72.6026 37 33 89.0
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Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan
Korean J Parasitol. 2016;54(4):439-446.   Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.439
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Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan
Korean J Parasitol. 2016;54(4):439-446.   Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.439
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Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan
Image Image Image Image
Fig. 1. The sampling sites of goats and sheep in Bhalwal, Kotmomin, Sahiwal, Shahpur, Silanwali, and Sargodha districts. (1) Bhalwal Tehsil is located in the eastern part of Punjab province of Pakistan. (2) Shahpur Tehsil lies on the Jhelum River. (3) Kot Momin is situated almost 40 km away from Sargodha tehsil. (4) Sahiwal tehsil is a subdivision (Tehsil) of Sargodha district. (5) Sargodha Tehsil is an administrative subdivision of Sargodha district.
Fig. 2. Showing the proportion of different sampling sites with goats and sheep of study area.
Fig. 3. Spatial trends of surfaces for the seroprevalence of toxoplasmosis in goats of urban areas.
Fig. 4. Spatial trends of surfaces for the seroprevalence of toxoplasmosis in sheep of urban areas.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan
Host Sex/Breed Examined (n) Infected (n) Seroprevalence (%) Statistical analysis; chi-square (χ²)
Goat Male 150 35 23.3 0.001
Female 380 192 50.5
Total 530 227 42.8
Sheep Male 72 16 22.2 0.003
Female 398 107 26.9
Total 470 123 26.2
Goat Beetal 167 81 48.5 0.021
Teddy 127 67 52.8
Kamori 121 58 48.0
Pahari 115 21 18.3
Total 530 227 42.8
Sheep Kajli 180 48 26.7 0.001
Desi 177 36 20.3
Damani 113 39 34.5
Total 470 123 26.2
Host Factors No. examined No. infected Seroprevalence (%) Statistical analysis; chi-square (χ²)
Goat Bhalwal 86 38 44.2 0.001
Sahiwal 105 46 43.8
Shahpur 74 19 25.7
Silanwali 148 53 35.8
Sargodha 117 71 60.7
Total 530 227 42.8
Sheep Bhalwal 113 18 15.9 0.002
Sahiwal 70 23 32.9
Shahpur 90 7 7.8
Silanwali 137 46 33.6
Sargodha 60 29 48.3
Total 470 123 26.2
Goat ≤ 1.5 yr 193 71 36.8 0.003
1.5-3 yr 122 49 40.2
≥ 3 yr 215 107 49.8
Total 530 227 42.8
Sheep ≤ 1.5 yr 206 27 13.1 0.004
1.5-3 yr 105 33 31.4
≥ 3 yr 159 63 39.6
Total 470 123 26.2
Hosts Districts Village Name Latitude Longitude No. examined No. infected Seroprevalence (%) No. examined No. infected Seroprevalence (%)
Sheep Bhalwal ChabaPurana 32.3382 72.9711 30 4 13.3 113 18 15.9
Liliani 32.208 72.955 25 7 28.0
KotMomin 32.1883 73.0286 30 2 6.6
Chowki Bhagat 32.049 72.9261 28 5 17.9
Sahiwal Farooka 31.8847 72.417 14 6 42.9 70 23 32.9
Sahiba Balochan 31.9403 72.4125 17 3 17.6
Kotla Mirbaz Khan 31.955 72.3748 21 6 28.6
Lakhiwal 31.9858 72.306 18 8 44.4
Shahpur Aaqil Shah 32.2762 72.4558 23 3 13.0 90 7 7.8
Jalpana 32.2617 72.5198 26 1 3.8
Bhakar Bar 32.3122 72.4924 20 5 25.0
Noon Wala 32.2669 72.3961 21 0 0.0
Silanwali Chak No. 99 N.B 31.9546 72.6351 34 16 47.1 137 46 33.6
Hundewali (Shaheen Abad) 31.922 72.6344 30 6 20.0
Chak 127 NB 31.835 72.5302 36 14 38.9
Basti Rasool Abad 31.8185 72.5468 37 10 27.0
Sargodha Chak 104 SB 31.9995 72.6961 21 4 19.0 60 29 48.3
Chak 36 NB 32.1261 72.7297 12 8 66.7
Jhal Chakian 32.1476 72.6417 10 4 40.0
Chak No. 90 NB 32.038 72.596 17 13 76.5
Goats Bhalwal Phularwan 32.3535 73.0141 22 9 40.9 86 38 44.2
Deowal 32.2151 72.9093 20 5 25.0
Chak 11 SB 32.159 72.9953 28 11 39.3
Mangowal 32.2151 72.8155 15 13 86.7
Sahiwal AzmatWala 31.8946 72.4045 25 9 36.0 105 46 43.8
Fateh Wala 31.9254 72.4356 29 17 58.6
Tibba 31.9606 72.3929 31 4 12.9
Deenpur 31.9683 72.2845 20 16 80.0
Shahpur Shahpur Sadar 32.2673 72.4762 16 2 12.5 74 19 25.7
Jalpana 32.2667 72.517 13 7 53.8
Rehmam Abad 32.3116 72.5332 19 1 5.3
Ichhar 32.2411 72.4218 26 9 34.6
Silanwali Chak No. 117 N.B 31.9358 72.6485 34 16 47.1 148 53 35.8
Chak No. 118 SB 31.9245 72.6469 37 3 8.1
Chak 126 NB 31.8483 72.5362 31 18 58.1
Islam Nagar 31.8208 72.5445 46 16 34.8
Sargodha Awan Abad 32.0011 72.719 24 11 45.8 117 71 60.7
Chak 33 NB 32.1237 72.7504 23 19 82.6
LoddyWala 32.1433 72.6611 33 9 27.3
Chak No. 95 NB 32.0491 72.6026 37 33 89.0
Table 1. The effect of species, sex, and breed on seroprevalence of toxoplasmosis
Table 2. The effect of district and age on seroprevalence of toxoplasmosis
Table 3. The effect of herds in different districts on seroprevalence of toxoplasmosis

Table 1.

Table 2.

Table 3.

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