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Original Article

Clinico-Epidemiological Patterns of Cutaneous Leishmaniasis Patients Attending the Anuradhapura Teaching Hospital, Sri Lanka

Lahiru Sandaruwan Galgamuwa1,2, Buthsiri Sumanasena3, Lalani Yatawara4, Susiji Wickramasinghe2, Devika Iddawela2,*
The Korean Journal of Parasitology 2017;55(1):1-7.
Published online: February 28, 2017

1Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Sri Lanka

2Department of Parasitology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka

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*Corresponding author: (devikaiddawela@yahoo.com)
• Received: September 29, 2016   • Revised: December 31, 2016   • Accepted: January 8, 2017

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  • Cutaneous leishmaniasis (CL) caused by Leishmania donovani is an endemic vector-borne disease in Sri Lanka. Over 2,500 cases have been reported since 2000 and the number of CL cases has dramatically increased annually. Total 57 clinically suspected CL patients attending the dermatology clinic in Anuradhapura Teaching Hospital were recruited from January to June 2015. Slit skin smears and skin biopsies were taken from each of the subjects. Clinical and epidemiological data were obtained using interviewer administered questionnaire. Forty-three (75.4%) patients among 57 were confirmed positive for L. donovani. The majority of infected patients was males (P=0.005), and the most affected age group was 21–40 years. Soldiers in security forces, farmers, and housewives were identified as high risk groups. The presence of scrub jungles around the residence or places of occupation (P=0.003), the presence of sandflies (P=0.021), and working outsides more than 6 hr per day (P=0.001) were significantly associated with CL. The number of lesions ranged from 1–3, and the majority (76%) of the patients had a single lesion. Upper and lower extremities were the prominent places of lesions, while the wet type of lesions were more prevalent in females (P=0.022). A nodular-ulcerative type lesion was common in both sexes. The presence of sandflies, scrub jungles, and outdoor activities contributed to spread of Leishmania parasites in an endemic pattern. Implementation of vector control programs together with health education with regard to transmission and prevention of CL are necessary to control the spread of this infection.
  • Key words: Leishmania donovani, cutaneous leishmaniasis, clinico-epidemiological pattern, Sri Lanka
Leishmaniasis is a vector-borne disease caused by a protozoan parasite of the genus Leishmania. The disease is transmitted by the bite of an infected female phlebotomine sandfly of the genera Phlebotomus and Lutzomia. Main clinical presentations of leishmaniasis include cutaneous, diffuse cutaneous, mucutaneous, and visceral. The main species responsible for visceral leishmaniasis (VL) is Leishmania donovani. Other species, including Leishmania tropica, Leishmania major, and Leishmania brasiliensis, cause cutaneous and muco-cutaneous leishmaniasis, respectively. The etiological agent responsible for cutaneous leishmaniasis (CL) in Sri Lanka has been identified as L. donovani of the zymodeme MON-37 [1], which is closely related to the causative organism of VL in the Indian subcontinent. The number of leishmaniasis patients has increased in the last few decades mainly due to human migration from non-endemic to endemic areas, poverty, deforestation, urbanization, and adaptation of the Leishmania parasites to additional vectors and mammalian hosts [2–4]. Leishmaniasis is endemic in 88 countries [5]. About 350 million people are at risk of contracting Leishmania infection, and as many as 12 million people in the world are believed to be currently infected with 1.5–2 million new cases being reported [6]. The diversity in the clinical manifestations and epidemiological characteristics of the disease depend on the invasiveness, pathogenicity, and tropism of the infected Leishmania species and the immune responses of the host [7].
In Sri Lanka, the first case of locally acquired CL was reported in 1992 [8]. Since then, CL cases have been increased, and the prevalence is slowly rising to epidemic proportions. Over 2,000 cases have been reported in the last decade in North Central, North Eastern, and Southern Provinces [9]. Since 2008, CL has been recognized as a notifiable disease by the Ministry of Health, Sri Lanka. According to the epidemiological data, Anuradhapura is one of the highest endemic districts for CL in Sri Lanka. However, clinical data and epidemiological factors associated with CL are scarce in Anuradhapura district. Therefore, the aim of the present study was to describe the clinical and epidemiological aspects of CL patients attending Anuradhapura Teaching Hospital.
Data collection
Anuradhapura is the largest district in Sri Lanka with an area of 7,179 km2. The estimated population was 856,232 in 2015 and more than 90% live in rural areas involving agriculture related activities. The annual rainfall in this area is about 1,240 mm with the average temperature ranging between 20°C and 30°C. Anuradhapura Teaching Hospital (ATH) is the only hospital with a leishmaniasis clinic in this district, and suspected CL cases are referred from peripheral hospitals to this clinic. Patients attending the dermatology clinic in ATH with lesions clinically suspected to have CL were included in this study from January to July 2015. Before collecting samples, written consent was obtained from all the patients who were willing to participate in the study. Individual records for each patient were made on an information sheet. Demographic data (age, sex, occupation, and area of residence) and clinical detail of the lesions (site, size, type of lesion, number of lesions, duration of the lesion, and inflammatory signs) were collected by an interviewer administered questionnaire. The past history of similar lesions in family members or neighbors and the presence of sandflies, rearing animals, and the presence of scrub jungles in the areas of their residence or place of occupation were also documented to determine the degree of exposure to the sandfly vector.
Sample collection and parasite identification
Samples were collected from each patient before starting treatment. A 3–4 mm nick at the edge of the lesion was made by a sterile scalpel, and the tissue fluid was smeared on a glass slide. When a patient has multiple lesions, several samples were obtained from each lesion. In addition, skin biopsy specimens were obtained from the active edge of the lesions by the dermatologists under sterile conditions. Biopsy samples were then stored in liquid nitrogen and transported to the Department of Parasitology, Faculty of Medicine for molecular studies. Smears on glass slides were air dried and fixed in methanol. The smears were stained with Giemsa and examined under oil immersion (×100) for Leishmania amastigotes.
DNA extraction and PCR
DNA was extracted from each skin tissue biopsy sample separately according to the manufacturer’s protocol using Pure Link™ Genomic DNA Mini Kit (Invitrogen Life Technologies, Carlsbad, California, USA). PCR was performed using the PCR mixture containing 5 μl of template DNA, 2.5 μl of 5× reaction buffer, 2.0 μl of 2.5 mM dNTPs, 2.0 μl of 25 mM MgCl2, 5 U/μl Taq DNA polymerase, and 1.5 μl of 10 μM of each primer (5′-CGGCTTCGCACCATGCGGTG-3′; 5′-ACATCCCTGCCCACATACGC-3′) in a total volume of 25 μl. Primers used in this study were amplified to 260 bp fragment specific to old world Leishmania spp. [10]. PCR amplifications were carried out in a thermocycler Thermolyne Amplitronyx™. The PCR conditions were 2 min at 95°C followed by 40 cycles of 30 sec at 94°C, 30 sec at 56°C, and 20 sec at 72°C. PCR products were electrophoresed on 1.5% agarose gels and visualized after ethidium bromide staining. CL was confirmed by positive findings from 1 of these 2 methods (microscopy and/or PCR).
Statistical analysis
The data were entered into a Microsoft Excel sheet, and were transferred into SPSS 20.0 for statistical analysis. Descriptive statistics and frequency distributions were performed to describe independent variables. Fisher’s exact test was used to determine the factors associated between exposure and clinical presentations with CL. P-values of less than 0.05 were considered as statistically significant.
Ethical clearance
Ethical approval was obtained from the Ethical Review Committee, Faculty of Medicine, University of Peradeniya, Sri Lanka. Permission to conduct the study was gained from the administration of the Anuradhapura Teaching Hospital. Written informed consent was obtained from each adult participant and from either of the parents or the legal guardian of children younger than 18 years of age.
Disease prevalence and socio-demographic characteristics
Total 57 CL suspected patients attending to Leishmania dermatology clinic in Anuradhapura Teaching Hospital were recruited for this study before starting their treatments. Out of the 57 samples, 38 (66.7%) were positive for Leishmania amastigotes by light microscopy, and 43 (75.4%) were clinically diagnosed and tested positive for L. donovani by PCR (Fig. 1). Five microscopy negative samples were identified as CL positive by PCR. Of CL positive patients, the majority (77%) were males. Ages of the participants ranged from 15 to 67 years (mean age, 37.8±12.6 years), and the majority of patients were aged between 21 to 40 years (Fig. 2). CL positivity of males was significantly higher than that of females (P=0.005).
CL infection was common among farmers and security service personnel (Table 1). These patients were from 5 administrative districts located in North and North Central Provinces in Sri Lanka. Most of the patients (32/43; 74%) were from rural areas of the Anuradhapura district. The rest was from Mullaithivu (8/43), Mannar (1/43), Vavunia (1/43), and Kilinochchi (1/43) districts. The majority (86%) of the patients had scrub jungles and sandflies around their residence or occupational places. A large proportion of CL patients (23/43) had infected family members or neighbors. None of the patients had overseas travel history. Therefore, it was assumed that all of them were infected locally. Existence of scrub jungles around residence or occupational places (P=0.003), presence of sandflies (P=0.021) and working outsides more than 6 hr per day (P=0.001) were significantly associated with CL. However, CL lesions in family members or neighbors, rearing animals, and living in rural or urban areas were not significantly associated with CL (P>0.05) (Table 2).
Clinical manifestations of CL patients
The majority (77%) had a single lesion while the rest had multiple lesions. The duration of cutaneous lesions ranged from 6 weeks to 4 years (mean duration, 6.0±4.3 months). Lesions were frequently seen on the upper limbs (42%) followed by lower limbs (32%), face (13%), and other parts of the body (Table 3), (Fig 3). No lesions were found on the soles, head, and genital areas. Diffuse leishmaniasis was not found in the current study. The size of the lesions ranged from 4 to 35 mm in diameter, and the majority of lesions had a surface area between 50–100 mm2. The commonest type of lesion was nodular-ulcerative in both sexes. More than a half (66%) had dry lesions. The presence of wet lesions was significantly more common in female patients compared to males (P=0.022).
This study showed that, among clinically diagnosed CL cases attending the Anuradhapura Teaching Hospital between January to July 2015, the majority had Leishmania amastigotes in their lesions indicating considerably high level of transmission in the North Central Province of Sri Lanka. According to our findings, adults were at a higher risk of contracting the infection due to their activities and/or occupation than children. This is in agreement with a previous report published in Sri Lanka [11]. CL was common among young adults aged between 21 to 40 years. Similar findings were reported in previous studies conducted in the North Central, North Western, and Central Provinces in Sri Lanka [12,13] and in India [14]. People in this age group are at a higher risk of exposure due to their high activity levels, occupation, and education. The highest prevalence in the Southern part of Sri Lanka was reported in patients aged 10–19 years [15].
Most of the patients in this study group had single lesions. Similar lesion pattern was reported in studies conducted in India and Tunisia. Lesions were more common in the upper limbs in our study. Siriwardena et al. [16] reported similar findings. The face and lower limbs had lesions similar to findings of other studies conducted in Sri Lanka [12] and in other countries [14,17]. These findings suggest that exposed parts of the body are more prone to sandfly bite. Lesions were not detected in the neck and waist area in female patients due to the covering of these areas by garments. In the present study, CL infection was higher among males than females. This is consistent with previous studies conducted in Sri Lanka [16,18,19]. Similar findings were reported in Libya, Pakistan, and Iran [20–22]. This observation could be because men are more likely to work in open environments, such as agricultural farms and fields, where they refrain from covering the upper half of their bodies and their limbs, thus increasing the risk of exposure to sandfly bites. Our results confirmed that people whose occupations are closely related to scrub jungles and forests are more prone to sandfly bites. The results are supported by previous studies conducted in the North and North Central Provinces, in Sri Lanka [23]. However, in India and Surinam, leishmaniasis was common among students and housewives [24,25]. Animal husbandry (rearing cows) is one of the main occupations in this area. In the present study, 42% of patients confirmed that rearing animals are abundant around their households. According to several studies, some animals (dogs and goats) have been identified as reservoir hosts for leishmaniasis [26,27]. However, the definite reservoir host has not been identified in Sri Lanka as of yet, although Leishmania amastigotes and antigens have been detected in dogs [22,28]. Almost all (93%) of the cases were from rural areas of the North and North Central Provinces. This could be due to the abundance of sandfly breeding sites, reservoir hosts, and natural habitats in the rural areas.
In the present study, the average time taken for the patient to present to dermatological clinic after acquiring the lesion was 6 months. The time taken to seek medical advice compares unfavorably to the mean average time reported by Aara et al. [29].
Dry type CL lesions were common in this study group, in agreement with previous studies conducted in Sri Lanka [12], India [30], Libya [31], and in Syria [32]. However, females had more wet lesions than males. In this study, nodulo-ulcerative lesions were the commonest type of lesion observed. This is in agreement with previous reports published in Sri Lanka [12]. The clinical appearance of cutaneous lesions varies depending of the immunity of the patient [33] and the species or strain of the causative agent [34]. The study reported a considerably large proportion of leishmaniasis patients among clinically suspected patients. However, we could not determine the prevalence of this infection in this area since we did not carry out active case detection. Therefore, further studies are needed to determine the prevalence, risk factors, and degree of exposure.
However, outdoor occupations and activities increase the risk of exposure to CL infections. The presence of sandflies and rearing animals contributes to the spread of Leishmania parasites in an endemic pattern. Therefore, implementation of vector control programs together with health education focused on the transmission and preventive methods of CL are necessary to limit the spread of this infection.

CONFLICT OF INTEREST

We have no conflict of interest related to this work.

The authors are thankful to the dermatologists in Anuradhapura Teaching hospital for their massive supports to get biopsy and smear samples. This study was funded by University of Peradeniya, Sri Lanka under grant no. RG/AF/2013/34.
Fig. 1
Ethidium bromide stained agarose gel of PCR products of Lesihmania species (CL) from patients. M, molecular marker (100 bp); lanes 1, 2, 4, 5, 6, and 8, positive samples; lanes 3 and 7, negative samples; lane 9, negative control; lane 10, positive control.
kjp-55-1-1f1.gif
Fig. 2
Age and sex distribution of CL patients.
kjp-55-1-1f2.gif
Fig. 3
Distribution of CL lesions on infected patients.
kjp-55-1-1f3.gif
Table 1
Occupational distribution of CL patients
Table 1
Occupation No. of CL patients %
Army soldiers 14 32.6
Farm workers 10 23.3
Housewives 9 20.9
Field officers 2 4.7
Teachers 2 4.7
Businessmen 2 4.7
Students 2 4.7
Sales executive 1 2.3
Carpenter 1 2.3
Total 43 100.0
Table 2
Frequency distribution of details of exposure
Table 2
Variables Categories CL positives (n=43) CL negatives (n=14) P-value
Presence of rear animals Yes 18 6 1.000
No 25 8
Presence of sandflies Yes 32 5 0.021
No 11 9
Presence of CL lesions in family members or neighbors Yes 23 4 0.132
No 20 10
Presence of scrub jungles around the residence/occupation place Yes 37 6 0.003
No 6 8
Outdoor activities more than 6 hr per day Yes 36 5 0.001
No 7 9
Dwelling area Rural 40 11 0.151
Urban 3 3
Table 3
Clinical presentations of CL patients
Table 3
Variables Categories Male patients Female patients Total (%) P-value
Age (year) 0–40 23 6 29 (67.4) 0.704
>40 10 4 13 (32.6)
Number of lesions 1 26 7 33 (76.7) 0.674
2 7 3 10 (23.3)
Sites of lesions Upper limbs 17 5 22 (41.5) 0.553
lower limbs 12 5 17 (32.1)
Face 4 3 7 (13.2)
Neck 4 0 4 (7.5)
Back 3 0 3 (5.7)
Duration of lesions (month) <6 23 9 32 (60.4) 0.886
6–12 13 3 16 (30.2)
>12 4 1 5 (9.4)
Size of lesions (mm2) 50–100 22 11 33 (62.3) 0.157
101–200 14 2 16 (30.2)
>200 4 0 4 (7.5)
Types of lesions Papulo-nodular 15 4 19 (35.8) 0.379
Nodular-ulcerative 16 8 24 (45.3)
Ulcers 9 1 10 (18.9)
Inflammatory signs Yes 30 10 40 (75.5) 1.000
No 10 3 13 (24.5)
Other features Dry CL 30 5 35 (66.0) 0.022
Wet CL 10 8 18 (34.0)
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Clinico-Epidemiological Patterns of Cutaneous Leishmaniasis Patients Attending the Anuradhapura Teaching Hospital, Sri Lanka
Korean J Parasitol. 2017;55(1):1-7.   Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.1
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Clinico-Epidemiological Patterns of Cutaneous Leishmaniasis Patients Attending the Anuradhapura Teaching Hospital, Sri Lanka
Korean J Parasitol. 2017;55(1):1-7.   Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.1
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Clinico-Epidemiological Patterns of Cutaneous Leishmaniasis Patients Attending the Anuradhapura Teaching Hospital, Sri Lanka
Image Image Image
Fig. 1 Ethidium bromide stained agarose gel of PCR products of Lesihmania species (CL) from patients. M, molecular marker (100 bp); lanes 1, 2, 4, 5, 6, and 8, positive samples; lanes 3 and 7, negative samples; lane 9, negative control; lane 10, positive control.
Fig. 2 Age and sex distribution of CL patients.
Fig. 3 Distribution of CL lesions on infected patients.

Fig. 1

Fig. 2

Fig. 3

Clinico-Epidemiological Patterns of Cutaneous Leishmaniasis Patients Attending the Anuradhapura Teaching Hospital, Sri Lanka

Occupational distribution of CL patients

Occupation No. of CL patients %
Army soldiers 14 32.6
Farm workers 10 23.3
Housewives 9 20.9
Field officers 2 4.7
Teachers 2 4.7
Businessmen 2 4.7
Students 2 4.7
Sales executive 1 2.3
Carpenter 1 2.3
Total 43 100.0

Frequency distribution of details of exposure

Variables Categories CL positives (n=43) CL negatives (n=14) P-value
Presence of rear animals Yes 18 6 1.000
No 25 8
Presence of sandflies Yes 32 5 0.021
No 11 9
Presence of CL lesions in family members or neighbors Yes 23 4 0.132
No 20 10
Presence of scrub jungles around the residence/occupation place Yes 37 6 0.003
No 6 8
Outdoor activities more than 6 hr per day Yes 36 5 0.001
No 7 9
Dwelling area Rural 40 11 0.151
Urban 3 3

Clinical presentations of CL patients

Variables Categories Male patients Female patients Total (%) P-value
Age (year) 0–40 23 6 29 (67.4) 0.704
>40 10 4 13 (32.6)
Number of lesions 1 26 7 33 (76.7) 0.674
2 7 3 10 (23.3)
Sites of lesions Upper limbs 17 5 22 (41.5) 0.553
lower limbs 12 5 17 (32.1)
Face 4 3 7 (13.2)
Neck 4 0 4 (7.5)
Back 3 0 3 (5.7)
Duration of lesions (month) <6 23 9 32 (60.4) 0.886
6–12 13 3 16 (30.2)
>12 4 1 5 (9.4)
Size of lesions (mm2) 50–100 22 11 33 (62.3) 0.157
101–200 14 2 16 (30.2)
>200 4 0 4 (7.5)
Types of lesions Papulo-nodular 15 4 19 (35.8) 0.379
Nodular-ulcerative 16 8 24 (45.3)
Ulcers 9 1 10 (18.9)
Inflammatory signs Yes 30 10 40 (75.5) 1.000
No 10 3 13 (24.5)
Other features Dry CL 30 5 35 (66.0) 0.022
Wet CL 10 8 18 (34.0)
Table 1 Occupational distribution of CL patients
Table 2 Frequency distribution of details of exposure
Table 3 Clinical presentations of CL patients

Table 1

Table 2

Table 3

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