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Original Article

Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea

Young Yil Bahk1, Yun-Kyu Park2, Byoung-Kuk Na3,4, Woon-Mok Sohn3, Sung-Jong Hong5, Jong-Yil Chai6, Tong-Soo Kim2,*
The Korean Journal of Parasitology 2018;56(4):335-339.
Published online: August 31, 2018

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*Corresponding author (tongsookim@inha.ac.kr)
• Received: March 21, 2018   • Revised: July 19, 2018   • Accepted: August 15, 2018

© 2018, Korean Society for Parasitology and Tropical Medicine

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  • This study was conducted to investigate the prevalence of intestinal parasites among students from Goseong-gun, Gangwon-do and Hadong-gun, Gyeongsangnam-do as typical low and high endemic counties. From May to July 2017, a total of 2,033 fecal samples were collected at 27 elementary, 10 junior high, and 8 high schools from 2 counties and examined by the Kato-Katz technique for egg-positive surveys (Collection rate: 37.02% [2,033/5,492]). Of the participants examined, 13 (0.64%) were found to harbor eggs of 3 parasitic species, Trichuris trichiura, Clonorchis sinensis and Metagonimus yokogawai. Based on the regional distribution, the egg-positive rate in Goseong-gun was 0% (0/550) and that in Hadong-gun was 0.88% (13/1,483). The positive rates for C. sinensis, M. yokogawai, and T. trichiura in Hadong-gun were 0.20% (3/1,483), 0.61% (9/1,483), and 0.07% (1/1,483), respectively. The present survey showed that the prevalence of parasitic infection among students is currently very low even in remote, previously endemic areas, and the present status of parasitic diseases can be summarized as some transmission of fish/food-borne trematodes. Thus, it is necessary to carefully, continuously monitor the trematode infection status, particularly of C. sinensis and M. yokogawai.
  • Key words: Intestinal helminthic, student, egg-positive, survey, endemic, non-endemic
Intestinal parasitic infections by helminths and protozoa are among the most prevalent infections worldwide [1]. Previously, Korea had a high intestinal helminth infection rate [2,3]. According to national-wide prevalence surveys, the egg-positive rate of helminthic infections was 90.5% in 1969, 84.3% in 1971, 63.2% in 1976, 41.1% in 1981, and 12.9% in 1986 [4,5]. However, Korea is one of the Asian countries that has successfully controlled intestinal parasitic infections through remarkable economic development, as demonstrated by national-wide surveys every 5 years since 1971. According to the 8th National-Wide Survey in Korea, a nation approved statistics program, despite the remarkable decrease of intestinal parasitic infections and soil-transmitted nematodes, the egg-positive rate was still 2.6% in 2012, fish/food-mediated parasite infections continue to persist or be detected, and infection with Trichuris trichiura, a soil-transmitted parasite, is gradually increasing [5]. Despite continued intestinal parasitic control projects and national surveys of human intestinal parasite prevalence, the estimated number of positive populations of some parasites, particularly Clonorchis sinensis and Gymnophalloides seoi, remains at 1 million cases. Therefore, studies of inhabitants in areas where intestinal parasites are known to be present as highly endemic foci or suspected endemic areas and their control regions should be investigated. In addition, the inhabitants of highly endemic areas should be systematically managed.
Thus, in this study, a complete survey of student groups in highly endemic and non-endemic areas was conducted to identify the present status of intestinal parasites infections and current pandemic status of the 2 groups through parasite-based analysis. National surveys do not provide accurate prevalence figures in endemic areas.
Study region
This study was conducted in Goseong-gun and Hadong-gun. These areas have a total land area of 664.2 and 675.5 km2 and populations of 29,110 and 48,533 as of July 2017, respectively. Goseong-gun, known as a parasite cleansing site traditionally, is in the northernmost frontier of Gangwon-do Administrative District (128°13′-128°35′ E and 38°11′–38°36′ N) and the East Sea. Forty-six percent of this land is restricted as a military protection area. Hadong-gun is in the southern tip of the Korean peninsula, in the southern part of Gyeongsangnam-do Administrative District (127°56′ E and 34°55′–35°19′ N). Hadong-gun is traditionally known as a region where intestinal parasites are prevalent. Especially in the case of Clonorchis sinensis, infection rate was 15–25%. This focus is adjacent to the Gwangyang-gun and Gurye-gun, Jeollanam-do Administrative Districts on the border of the Seomjin river, with Jinju and Sacheon-si to the east and Namhae-gun to the south.
Subjects
This study was conducted with extensive cooperation from local schools comprised of elementary, junior high, and high school students. Participants in Goseong-gun were from 13 elementary schools, 4 junior high schools, and 4 high schools, while those in Hadong-gun were from 14, 6, and 4 schools, respectively. Table 1 presents data for the subjects in this study and collection rates by school year and schools.
Stool sample collection, mass stool examination and treatment
A total of 2,033 fecal samples were collected from the study population: 550 from Goseong-gun (Collection rate: 550/2,433 [22.6%]) and 1,483 from Hadong-gun (1,483/3,059 [48.48%]). The samples were examined using the medical general laboratory method described in the WHO Bench Aids for the Diagnosis of Intestinal Parasites. The sediment was examined under a light microscope using the Kato-Katz technique [6]. One Kato-Katz slide was prepared for each sample. Microscopic reading of the slides was conducted on the same day of processing approximately 2 hr after preparation, particularly to detect hookworm eggs which may be affected by the clearing effect of glycerin. Helminth eggs were identified by their size and distinctive morphological features. Raw egg counts were multiplied by 21, a constant value, to derive the number of eggs per gram (EPG) in the stool. Experienced microscopists from the Department of Parasitology and Tropical Medicine, Inha University processed and examined the stool specimens. Intestinal parasite species examined in this study were Ascaris lumbricoides, Ancylostoma duodenale, Trichuris trichiura, Trichostrongylus orientalis, Enterobius vermicularis, C. sinensis, Paragonimus westermani, Metagonimus yokogawai, Gymnophalloides seoi, Diphyllobothrium latum, and Taenia spp.
Ethical considerations
The study procedures involving human stool sample collection, laboratory investigation, interviews, and questionnaires were reviewed and approved by the Institutional Review Board of Inha University and conducted according to the principles expressed in the 1964 Helsinki Declaration. All participants who agreed to participate in this study were asked to read through the participant information sheet explaining the objectives, procedures, possible risks, and benefits of the research project. Signed consent forms were obtained from participants themselves and their parents or student’s guardians prior to fecal sample submission and personal information talking was obtained through interviews and questionnaires.
Demographic information of participants
Through interviews and information in schools in Goseong-gun and Hadong-gun, data was obtained for 2,033 participants who underwent parasitological screening. A complete survey of the entire group of students in the highly endemic area of parasitic infection and those in the non-parasitic endemic area was carried out as a control group. These participants represented the young population in the 2 counties. Sampling of the participants was set up for all elementary (Goseong: 13 schools, and Hadong: 14), junior high (Goseong: 4 and Hadong: 6), and high (Go seong: 4 and Hadong: 4) schools within the survey areas. The distributions of the number of students were relatively uniform (Table 1).
Positive rate and characteristics of intestinal parasites
Of the 2,033 examined fecal samples from the surveyed participants, 13 were found to contain intestinal parasite eggs and the overall prevalence of parasitic infestations was 0.64%. Eggs of 3 parasitic species were identified, including 1 nematode species (Trichuris trichiura) and 2 trematode species (C. sinensis and M. yokogawai). The most prevalent intestinal parasite found in the examined stools was M. yokogawai (9/13 cases, 69.23%). The detailed results are shown in Tables 2, 3. Based on the regional distribution of the parasitic infections, the positive rate of parasitic infections in Goseong-gun was 0% (0/550 participants surveyed) and that in Hadong-gun was 0.88% (13/1,483). The egg-positive rates of fecal samples from Hadong-gun were 0.23% (2/852 participants) in elementary schools, 1.66% (5/301) in junior high schools, and 1.82% (6/330) in high schools, respectively. The positive rates for C. sinensis and M. yokogawai were 0.20% and 0.61%, respectively. The mean intensities of infection were 27 EPG for C. sinensis, 94 for M. yokogawai, and 2 for T. trichiura (Table 4). Performing fecal egg counts and conducting follow-up tests would have provided a more accurate picture of parasite management issues. For the classification of egg counts, 5 EPG gradings are used (I–IV) as important epidemiological indicators: (I: 21–999, II: 1,000–4,999, III: 5,000–9,999, IV: 10,000–29,999, and V: >30,000). A fecal egg count below grade III is considered light, grade IV is considered moderate range, and values above 30,000 are considered heavy. The mean EPG of 13 positive participants was 71.6, which represents early grade I (Table 4).
Korea was previously shown to be heavily endemic for various species of parasitic infestations for a long time. In this study, we selected 2 counties as low and high endemic counties. According to the 8th National-Wide Survey in Korea, the estimated positive rate in the Gangwon-do Administrative District was 0.0867%, showing the lowest egg-positive rate [5]. The estimated positive rate in the Gyeongsangnam-do Administrative District was 6.98%, which was the 3rd highest level, and the estimated number of infected individuals was approximately 227,000. Seomjin river is a major river, and the egg-positive rate of population living in the river basins is high [7].
The present study involved surveys of intestinal parasites in 2 counties (Goseong-gun and Hadong-gun) as low and high endemic counties in Korea and revealed a considerably low prevalence of intestinal parasites (average of 0.64% in total participants, 0% in Goseong-gun, and 0.88% in Hadong-gun) among students, representing the young generation from the 2 counties. Human helminthiasis in Korea has been heavily endemic for a long time and is distributed along most river basins throughout the country. The rapid decrease in the overall prevalence of intestinal parasites over the past several decades has been achieved through the continuous efforts of a nationwide control program by the Korean government that mostly addressed students. In this study, the egg-positive case in Goseong-gun was not accurately determined. However, an apparent trend was revealed by the results. The prevalence of M. yokogawai was slightly higher than that of C. sinensis. This finding differs from the general trends. According to the 7th and 8th National-Wide Surveys in Korea, the total egg-positive rates of fish/food-borne trematodes, particularly C. sinensis, M. yokogawai, and G. seoi, were 2,89% in the 7th National Survey and 2.14% in the 8th National-Wide Survey. In the 8th National-Wide Survey at 2012, the positive rates of C. sinensis, M. yokogawai, and G. seoi were found to be 1.86%, 0.26%, and 0.02%, respectively. In addition, the egg-positive rate of nematode, T. trichiura, was also extremely low (0.07% over 0.41% in the 8th National-Wide Survey [1/1,483 cases in Hadong-gun]). This result suggests that this nematode has been nearly eliminated. In fact, T. trichiura as well as C. sinensis and M. yokogawai are representative intestinal parasites in Korea [5]. This parasite had been not required management since the infection rate was continuously decreased according to the 6th National-Wide Survey (65.4% in the 1st National-Wide Survey [1971], 42.0% [1976], 23.4% [1981], 4.8% [1986], 0.2% [1992], and 0.04% in surveys in subsequent years [1997]), but increased again according to the 7th National-Wide Survey (0.27%) and 8th National-Wide Survey (0.41%). The risk factors of T. trichiura infection in Korea remain unclear. Although this infection is not currently a serious concern, it is necessary to carefully and continuously monitor the infection status.
For age-dependent egg-positive rates, although the trend in egg-positive patterns according to age showed some similarities between this study and the national surveys, there was a considerable decrease in the egg-positive rate of fish/food-borne trematodes compared to that reported by the 8th National-Wide Survey. In the 8th National-Wide Survey, the age-specific positive rate was generally low in individuals less than 10 years old, but this rate increased during the teenage years. For C. sinensis, the egg-positive rate in those less than 10 years old was 0.11%, while that in teenagers was 0.47% [5]. In contrast, individuals in their 20s, 30s, 40s, and 50s showed rates of 2.04%, 2.16%, 3.48%, and 4.26%. Our survey showed that the egg-positive rates of student groups for C. sinensis and M. yokogawai in Hadong-gun were 0.20% and 0.61%, respectively, which are remarkably lower rate than those reported in the 7th and 8th National-Wide Surveys. Generally, the infection rate of C. sinensis has the largest influence on the overall nationwide parasite-positive rate, as this liver fluke infection typically accounts for the largest proportion of infections in Korea [7]. It is well-known that fish/food-borne parasitic infections including C. sinensis are high in riverside regions. The risk factors for C. sinensis infestation in Korea have been widely examined in epidemiological studies. Particularly, in Hadong-gun, where residents consume large amounts of fish and utilize unique cuisine methods, the infection-positive rate for C. sinensis is higher than that in other regions of Korea because of differences in eating patterns and elimination of previously prevalent helminthiases throughout the country [8]. Metagonimus yokogawai is a fish-borne trematode that is medically important in Korea. Since Yeo and Seo reported a high endemic area of M. yokogawai near Seomjin river, numerous surveys have revealed endemic areas along the southern and eastern coasts of Korea where the sweet fish (Plecoglossus altivelis) is available [2,9]. This species is hermaphroditic and capable of self-fertilization. Embryonated eggs are passed into the aquatic environment (fresh or brackish water), each containing a fully developed larva known as a miracidium.
The prevalence of the parasites determined in this study do not reflect the actual status of infection because the examination was performed only using the Kato-Katz technique.
In conclusion, the present survey shows that the prevalence of parasitic infection is currently very low even in remote, previously endemic areas and the present status of parasitic diseases in students from the surveyed counties revealed some transmission of fish/food-borne trematodes, as previously described [8]. Currently, C. sinensis and M. yokogawai are 2 major helminthes with a pattern of parasitic diseases that has changed in a manner commensurate with environmental and society-based changes as well as with economic development. These helminths are transmitted by freshwater fishes, and local populations prefer to consume raw fish as recreation or epicureanism. Infections are contracted by eating undercooked fishes that use metacercariae. Contaminated fishes are mainly found in streams and rivers where freshwater snails thrive. Thus, it is necessary to carefully and continuously monitor the trematode infection status, particularly of C. sinensis and M. yokogawai.

CONFLICT OF INTEREST

We have no competing interest related to this work.

We are grateful to all blood donors and the staff of the Public Health Centers in Goseong-gun and Hadong-gun. This study was supported by a grant from the Korea Association of Health Promotion (2017-02) and by the National Research Foundation of Korea (NRF) grants funded by the Korea Government (NRF-2017M3A9B8069530) in sample collection.
Table 1
Subject of investigation and collection rate of fecal samples from each county by school grade
Table 1
Area Grade Elementary School Junior High School High School Total
1st 2nd 3rd 4th 5th 6th Total 1st 2nd 3rd Total 1st 2nd 3rd Total
Goseong Subject 181 181 177 172 182 154 1,047 152 183 195 530 287 295 274 856 2,433
Collection (Rate) 79 (43.6) 95 (52.5) 67 (37.9) 81 (47.1) 45 (24.7) 52 (33.8) 419 (40.0) 50 (32.9) 25 (13.7) 11 (5.6) 86 (16.2) 9 (3.1) 23 (7.8) 13 (4.7) 45 (5.3) 550 (22.6)
Hadong Subject 282 258 272 287 299 285 1,683 208 234 230 672 274 219 211 704 3,059
Collection (Rate) 168 (59.6) 136 (52.7) 157 (57.7) 125 (43.6) 145 (48.5) 121 (42.5) 852 (50.6) 92 (44.2) 99 (42.3) 110 (47.8) 301 (44.8) 128 (46.7) 123 (56.2) 79 (37.4) 330 (46.9) 1,483 (48.5)
Table 2
Number of helminth egg-positive cases by school grades in this survey
Table 2
Positive No. Elementary School Junior High School High School Total
1st 2nd 3rd 4th 5th 6th 1st 2nd 3rd 1st 2nd 3rd
Goseong 0 0 0 0 0 0 0 0 0 0 0 0 0
Hadong 0 0 0 0 0 2 1 4 0 0 2 4 13
Subtotal 0 0 0 0 0 2 1 4 0 0 2 4 13
Table 3
Number of helminthic egg-positive stools
Table 3
No. of samples No. of egg-positive cases Positive rate Parasite
Nematoda Trematoda Cestoda
Al* H Tt To Ev Cs Pw My Gs T spp. Dl
Goseong 550 0 0 0 0 0 0 0 0 0 0 0 0 0
Hadong 1,483 13 0.88 0 0 1 (0.07) 0 0 3 (0.20) 0 9 (0.61) 0 0 0

*Al, Ascaris lumbricoides; H, Hookworm; Tt, Trichuris trichiura; To, Trichostrongylus orientalis; Ev, Enterobius vermicularis; Cs, Clonorchis sinensis; Pw, Paragonimus westermani; My, Metagonimus yokogawai; Gs, Gymnophalloides seoi; T spp., Taenia spp.; Dl, Diphyllobothrium latum.

Table 4
Number and average of excreted eggs in the egg-positive stools
Table 4
No. of positive cases No. of total excreted eggs Average of the excreted eggs
Clonorchis sinensis 3 82 27
Metagonimus yokogawai 9 847 94
Trichuris trichiura 1 2 2
Total 13 931
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Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea
Korean J Parasitol. 2018;56(4):335-339.   Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.335
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Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea
Korean J Parasitol. 2018;56(4):335-339.   Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.335
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Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea
Survey on Intestinal Helminthic Infection Status of Students in Two Counties, Hadong-gun and Goseong-gun, Korea

Subject of investigation and collection rate of fecal samples from each county by school grade

Area Grade Elementary School Junior High School High School Total
1st 2nd 3rd 4th 5th 6th Total 1st 2nd 3rd Total 1st 2nd 3rd Total
Goseong Subject 181 181 177 172 182 154 1,047 152 183 195 530 287 295 274 856 2,433
Collection (Rate) 79 (43.6) 95 (52.5) 67 (37.9) 81 (47.1) 45 (24.7) 52 (33.8) 419 (40.0) 50 (32.9) 25 (13.7) 11 (5.6) 86 (16.2) 9 (3.1) 23 (7.8) 13 (4.7) 45 (5.3) 550 (22.6)
Hadong Subject 282 258 272 287 299 285 1,683 208 234 230 672 274 219 211 704 3,059
Collection (Rate) 168 (59.6) 136 (52.7) 157 (57.7) 125 (43.6) 145 (48.5) 121 (42.5) 852 (50.6) 92 (44.2) 99 (42.3) 110 (47.8) 301 (44.8) 128 (46.7) 123 (56.2) 79 (37.4) 330 (46.9) 1,483 (48.5)

Number of helminth egg-positive cases by school grades in this survey

Positive No. Elementary School Junior High School High School Total
1st 2nd 3rd 4th 5th 6th 1st 2nd 3rd 1st 2nd 3rd
Goseong 0 0 0 0 0 0 0 0 0 0 0 0 0
Hadong 0 0 0 0 0 2 1 4 0 0 2 4 13
Subtotal 0 0 0 0 0 2 1 4 0 0 2 4 13

Number of helminthic egg-positive stools

No. of samples No. of egg-positive cases Positive rate Parasite
Nematoda Trematoda Cestoda
Al* H Tt To Ev Cs Pw My Gs T spp. Dl
Goseong 550 0 0 0 0 0 0 0 0 0 0 0 0 0
Hadong 1,483 13 0.88 0 0 1 (0.07) 0 0 3 (0.20) 0 9 (0.61) 0 0 0

*Al, Ascaris lumbricoides; H, Hookworm; Tt, Trichuris trichiura; To, Trichostrongylus orientalis; Ev, Enterobius vermicularis; Cs, Clonorchis sinensis; Pw, Paragonimus westermani; My, Metagonimus yokogawai; Gs, Gymnophalloides seoi; T spp., Taenia spp.; Dl, Diphyllobothrium latum.

Number and average of excreted eggs in the egg-positive stools

No. of positive cases No. of total excreted eggs Average of the excreted eggs
Clonorchis sinensis 3 82 27
Metagonimus yokogawai 9 847 94
Trichuris trichiura 1 2 2
Total 13 931
Table 1 Subject of investigation and collection rate of fecal samples from each county by school grade
Table 2 Number of helminth egg-positive cases by school grades in this survey
Table 3 Number of helminthic egg-positive stools

Al, Ascaris lumbricoides; H, Hookworm; Tt, Trichuris trichiura; To, Trichostrongylus orientalis; Ev, Enterobius vermicularis; Cs, Clonorchis sinensis; Pw, Paragonimus westermani; My, Metagonimus yokogawai; Gs, Gymnophalloides seoi; T spp., Taenia spp.; Dl, Diphyllobothrium latum.

Table 4 Number and average of excreted eggs in the egg-positive stools

Table 1

Table 2

Table 3

Table 4

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