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Brief Communication

Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam

Nguyen Thi Hong Chien1, Thi Lan Nguyen2, Khanh Linh Bui1, Tho Van Nguyen1, Thanh Hoa Le3,4,*
The Korean Journal of Parasitology 2019;57(1):43-47.
Published online: February 26, 2019

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*Corresponding author (imibtvn@gmail.com)
• Received: November 11, 2018   • Revised: January 23, 2019   • Accepted: January 28, 2019

Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
Korean J Parasitol. 2019;57(1):43-47.   Published online February 26, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.1.43
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Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
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Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam
Image Image
Fig. 1 Sample collection sites and a micrograph of a stained blood smear from cattle. (A) Map showing the locations of Ha Noi and Hai Duong Provinces in northern Vietnam (solid circle symbols). (B) Anaplasma marginale in erythrocytes (arrows) in a blood sample from cattle. Giemsa stain (×1,000).
Fig. 2 Phylogenetic tree showing taxonomic relationships of Anaplasma marginale detected from cattle and A. platys from cattle and dogs, in 2 provinces in northern Vietnam. The relationships were inferred based on 16S rDNA data (501–502 bp) from 26 sequences, including 10 from Vietnam and 15 reference sequences of Anaplasma species. Ehrlichia canis was used as an outgroup. Phylogenetic reconstruction was performed in MEGA7 using maximum likelihood analysis based on the general time-reversible model. Support for each node was indicated by 1,000 bootstrap resamplings [18]. The scale bar represents the number of substitutions per site. For each sequence, the full species names are followed up by strain abbreviations (in brackets) and the country (where they were isolated). The accession numbers are given at end of each sequence label. Ten Anaplasma spp. from Vietnam are marked with a solid circle. Names of hosts (cattle or dogs, in brackets) are added in the label for every sequence from Vietnam.

Fig. 1

Fig. 2

Anaplasma marginale and A. platys Characterized from Dairy and Indigenous Cattle and Dogs in Northern Vietnam

Molecular identity of Anaplasma spp. from indigenous and dairy cattle and domestic dog in 2 provinces in northern Vietnam

No. Hosts Date of isolation Geographical locality of collection Sample code Species identification GenBank No.
1 Indigenous cattle 12-Dec-16 Ba Vi district, Ha Noi ANA25BV Anaplasma marginale MH686041
2 Indigenous cattle 12-Dec-16 Ba Vi district, Ha Noi ANA88 Anaplasma marginale MH686042
3 Hybrid dairy cattle 05-Sep-17 Ba Vi district, Ha Noi BS27 Anaplasma marginale MH686043
4 Hybrid dairy cattle 05-Sep-17 Ba Vi district, Ha Noi BS255 Anaplasma marginale MH686044
5 Indigenous cattle 05-Aug-17 Ba Vi district, Ha Noi BV1 Anaplasma marginale MH686045
6 Indigenous cattle 08-Aug-17 Kim Thanh district, Hai Duong BVHD1 Anaplasma marginale MH686046
7 Indigenous cattle 08-Aug-17 Kim Thanh district, Hai Duong BVHD2 Anaplasma marginale MH686047
8 Indigenous cattle 12-Dec-16 Ba Vi district, Ha Noi city ANA111 Anaplasma platys MH686048
9 Indigenous cattle 08-Aug-17 Kim Thanh district, Hai Duong BVHD4 Anaplasma platys MH686049
10 Domestic dog 10-Jan-17 Ba Vi district, Ha Noi C9BV Anaplasma platys MH686050
Table 1 Molecular identity of Anaplasma spp. from indigenous and dairy cattle and domestic dog in 2 provinces in northern Vietnam

Table 1

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