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Acanthamoeba in Southeast Asia ? Overview and Challenges

Chooseel Bunsuwansakul1, Tooba Mahboob2, Kruawan Hounkong3, Sawanya Laohaprapanon4, Sukhuma Chitapornpan5, Siriuma Jawjit4, Atipat Yasiri6, Sahapat Barusrux1, Kingkan Bunluepuech1, Nongyao Sawangjaroen7, Cristina C. Salibay8, Chalermpon Kaewjai9, Maria de Lourdes Pereira10, Veeranoot Nissapatorn1,*
The Korean Journal of Parasitology 2019;57(4):341-357.
Published online: August 31, 2019

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*Corresponding author (nissapat@gmail.com)
• Received: April 21, 2019   • Revised: July 18, 2019   • Accepted: July 18, 2019

Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Acanthamoeba in Southeast Asia ? Overview and Challenges
Korean J Parasitol. 2019;57(4):341-357.   Published online August 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.4.341
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Acanthamoeba in Southeast Asia ? Overview and Challenges
Korean J Parasitol. 2019;57(4):341-357.   Published online August 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.4.341
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Acanthamoeba in Southeast Asia ? Overview and Challenges
Image
Fig. 1 Epidemiology and clinical cases of Acanthamoeba infection in Southeast Asia. B: Granulomatous amoebic encephalitis; E: Acanthamoeba keratitis; N: Acanthamoeba sinusitis; and S: Gastric acanthamoebiasis.

Fig. 1

Acanthamoeba in Southeast Asia ? Overview and Challenges

Distribution of environmental Acanthamoeba spp. in Southeast Asia

Country Type of samples No. of sample Positive culture Acanthamoeba spp. morphology References
FLA Acanthamoeba spp. Group I Group II Group III
Thailand Water
 Water samples 95 51.58% (49/95) 18.95% (18/95) ND ND ND Nacapunchai et al. (2001) [23]
 Hot spring water 69 37.68% (28/69) 13% (9/69) ND ND ND Lekkla et al. (2005) [17]
 Freshwater pond and irrigation canals 84 ND 19.05% (16/84) 15.79% (3/19) 84.21% (16/19) NF Nuprasert et al. (2010) [59]
 Flood water 7 100% (7/7) 14.29% (1/7) ND ND ND Wannasan et al. (2013) [60]
 Freshwater pond in public parks 300 ND 35% (105/300) 23.36% (25/105) 73.83% (79/105) 2.8% (3/105) Buppan et al. (2018) [61]
 Water-logged fields 2 100% (2/2) 100% (2/2) ND ND ND Wannasan et al. (2009) [62]
 Ditches 4 100% (4/4) NF ND ND ND
 Paddy fields 6 100% (6/6) 16.67% (1/6) ND ND ND
 Fish farms 10 50% (5/10) 10% (1/10) ND ND ND
 Large pond 6 50% (3/6) NF ND ND ND
 Natural water 63 ND 15.87% (10/63) ND ND ND Thammaratana et al. (2016) [15]
Air
 Outdoor air 103 ND 41.7% (43/103) NF 16.5% (17/43) 15.5% (16/43) Yaicharoen et al. (2007) [63]
 Indoor air 64 ND 18.1% (37/64) NF 13.7% (28/37) 2.9% (6/37)
Soil
 Soil swab samples 120 69.17% (83/120) 33.33% (40/120) ND ND ND Nacapunchai et al. (2001) [23]
 Water-logged fields 2 100% (2/2) 50% (1/2) ND ND ND Wannasan et al. (2009) [62]
 Ditches 4 75% (3/4) 50% (2/4) ND ND ND
 Paddy fields 6 100% (6/6) NF ND ND ND
 Fish farms 10 50% (5/10) NF ND ND ND
 Large pond 6 66.7% (4/6) 16.67% (2/6) ND ND ND
Malaysia Water
 Domestic tap water 42 ND 2.4% (1/42) ND ND ND Anisah et al. (2003) [64]
 Swimming pools in Kuala Lumpur 840 54.4% (457/840) 46.19% (388/840) Positive Positive ND Init et al. (2010) [32]
 Recreational anthropogenic lake A 7 ND 100% (7/7) ND ND ND Onichandran et al. (2013) [16]
 Recreational anthropogenic lake B 6 ND 100% (6/6) ND ND ND
 Tap water 181 29.8% (54/181) 24.9% (45/181) ND ND ND Gabriel et al. (2019) [65]
 Recreational places 57 66.7% (38/57) 70.2% (40/57) ND ND ND
 Water dispenser units 3 33.3% (1/3) 66.7% (2/3) ND ND ND
 Filtered water 4 75% (3/4) NF ND ND ND
 Drain water 1 100% (1/1) NF ND ND ND
 Paddy fields 4 50% (2/4) 100% (4/4) ND ND ND
 Drinking water treatment 61 90.2% (55/61) 18.03% (7/11) ND ND ND Richard et al. (2016) [20]
 Water samples 15 ND 100% (15/15) ND ND ND Basher et al. (2018) [24]
 Swabs (rocks and stones) 15 ND 73.33% (7/11) ND ND ND
Soil
 Wet soil 15 ND 100% (15/15) ND ND ND
 Children playgrounds (Dry soil) 15 ND 100% (15/15) ND ND ND
Other
 Indoors wall surface 20 ND 100% (20/20) ND ND ND
 Outdoor wall surface 20 ND 100% (20/20) ND ND ND
 Air conditioners in KM 87 ND 23% (20/87) NF 71.43% (15/21) 28.57% (6/21) Chan et al. (2011) [66]
The Philippines Water
 River 10 ND 30% (3/10) ND ND ND Onichandran et al. (2014) [67]
 Swimming pools 4 ND 50% (2/4) ND ND ND
 Pond 3 ND 66.67% (2/3) ND ND ND
 Lake 6 ND 33.33% (2/6) ND ND ND
 Tap water 3 ND 33.33% (1/3) ND ND ND
 Rain/tap tank 2 ND NF ND ND ND
 Water dispenser 2 ND 50% (1/2) ND ND ND
 Well 1 ND 100% (1/1) ND ND ND
 Spring 1 ND NF ND ND ND
 Mineral 1 ND NF ND ND ND
 Water 3 ND 100% (3/3) ND ND ND Rivera and Adao (2008) [29]
Soil
 Soil 10 ND 100% (10/10) ND ND ND Rivera and Adao (2008) [29]
 Soil 4 ND 100% (4/4) ND ND ND Cruz and Rivera (2014) [25]
Other
 Contact lens storage cases 4 ND 100% (4/4) ND ND ND Rivera and Adao (2008) [29]
Vietnam Soil
 Garden soil 1 359 small sub unit rDNA Sequences of Amoebae 5.95% ND ND ND Denet et al. (2017) [21]
 Mining soil 1 4.76% ND ND ND
Others (Lao PDR, Myanmar, and Singapore) Treated water in Lao PDR 9 11.11% (1/9) NF ND ND ND Majid et al. (2017) [14]
Untreated water in Lao PDR 22 4.55% (1/22) 4.55% (1/22) ND ND ND
Treated water in Yangon 11 18.18% (2/11) NF ND ND ND
Untreated water in Yangon 31 16.13% (5/31) 9.68% (3/31) ND ND ND
Treated water in Singapore 6 NF NF ND ND ND
Untreated water in Singapore 15 NF NF ND ND ND

ND, Not detected; NF, Not found.

Examples of Acanthamoeba infection cases in Southeast Asia

Ethnicity/Gender Age (yr) Clinical sample Diagnostic method Condition (Genotype) Potential history of patients Treatment Status after treatment References
Singaporean male 28 Corneal scraping Microscopy and culture AK with Pseudomonas aeruginosa Hit with polyvinylchloride pipe, topical steroids Before diagnosis: cefazolin and gentamicin; After diagnosis: topical cycloplegics; topical 0.1% hexamidine, 0.02% chlorohexidine, and transplantation Vision blurriness Lim et al. (2018) [68]
48/200 felines and 8/25 canines (56/225 naturally-infected animal) in Malaysia Adults and juveniles Corneal swabs Microscopy, culture, and partial 18S rDNA sequencing AK (T4) Dry soil and dust (strain-matched partial 18S rDNA sequence) - - Basher et al. (2018) [24]
Indonesian female 32 Corneal scraping Microscopy and culture AK Monthly disposable soft contact lens wearer for 1 year with tap water to rinse contact lens and case in many occassions Before diagnosis: Steroid eye drops, Moxifloxacin eye drops, natamycin eye drops, polymyxin-neomycin-gramicidin eye drops (Polygran®), and voriconazole eye drops After diagnosis: propamidine isethionate (Brolene®) and Polygran® Improved vision blurriness Muslim et al. (2018) [19]
Thai female 58 Brain abscess CT scan, Microscopy, and PCR on partial 18S rDNA sequencing GAE Farmer with pulmonary tuberculosis history, Raynaud’s phenomenon, mild myositis, and high antinuclear antibody (speckle type) Metronidazole and Prednisolone Loss of follow-up Wara-Asawapati et al. (2017) [22]
Indonesian male 2 Cerebrospinal fluid CT scan and microscopy GAE Drowning survivor Intravenous ceftazidime, metronidazole, fluconazole and rifampicin Alive with altered mental status Gunawan et al. (2016) [69]
Filipino male 76 Corneal scraping Microscopy, culture, and partial 18S rDNA sequencing AK (T4) Non-contact lens wearer Chlorhexidine Corneal scar Buerano et al. (2014) [27]
12/180 Filipinos - Nasal swab Microscopy, culture, and partial 18S rDNA sequencing −(T5, 54, T11) Street sweeper (4/44), Garbage collector (2/37), Garbage sorter (0/16), Landscaper (1/6), Bioreactor laborer (0/4), foremen and superviors (0/3), and students (1/70) - - Cruz and Rivera (2014) [25]
22 cases in Siriraj hospital, Thailand (1996–2006) 48.3±14.5 for 8 non-contact lens wearers, 30.6±15.3 for 12 contact lens wearers Corneal scraping Microscopy and culture AK Contact lens wearer with lack of hygiene Chlorhexidine, polyhexamethylene biguanide or propamidine Improved vision blurriness and loss of follow-up for some patients Wanachiwanawin et al. (2012) [70]
9/103 infective keratitis patients with eye surgery - - - AK - Polyhexamethylbiguanide, chlorohexidine, propamidine dexamethasone, hexamidine, and transplantation Improved vision blurriness Anshu et al. (2009) [71]
22 Chinese, 8 Malay, 5 Indian, 7 others (2005–2007 in Singapore) <20 years-old=13, 21–40 years-old=25, 41–60 years-old=4 Corneal scraping, biopsy, and keratoplasy specimen Microscopy and culture AK Suboptimal hygiene practices 0.02% topical polyhexamethylene niguanide, 0.02% chlorohexidine, 0.1% hexamidine, 0.1% propamidine isethionate, and transplantation. Vision blurriness Por et al. (2009) [72]
3 Filipinos - Corneal scraping Microscopy AK Non-contact lens wearer 0.1% topical diclofenac sodium and atropine drops. Vision blurriness in 2/3 patients Agahan et al. (2009) [73]
3 AK patients/127 microbial keratitis eyes (2001–2004) in Ramathibodi Hospital, Thailand Mean age 40±22 for all 127 microbial keratitis patients Corneal scraping Microscopy and culture AK Contact lens wearers - - Sirikul et al. (2008) [74]
Chinese female 13 Corneal scraping Microscopy and culture AK Rigid gas-permeable contact lens wearer Before diagnosis; Acantamoebic agents: 0.02% topical polyhexamide methylene biguanide, 0.02% chlorohexidine, 0.1% hexamidine, and transplantation. After surgery: 0.1% topical dexamethasone phosphate, 0.5% levofloxacin, same Acanthamoebic agents, and topical preservative-free steroids. Improved vision blurriness Parthasarathy and Tan (2007) [75]
Thai female - Biopsy and autopsy Microscopy GAE Swimming in a dam - Death Siripanth (2005) [76]
Thai male 36 Nasal exudate Microscopy and culture Amoeba co-infection sinusitis (Naegleria sp. and Acanthamoeba sp.) Diving in a natural pond Caldwell-Luc operation, Intravenous amphotericin B, oral ketoconazole, and amoxycillin/clavulanic acid Cured Sukthana et al. (2005) [77]
Singaporean female 39 Corneal scraping Microscopy and culture AK Contact lens wearer with multipurpose disinfectant solution Misdiagnosis: Occulentum Acyclovir, Guttae Choramphenicol, and 0.12% Guttae Prednisolone; After diagnosis: 0.1% gutt propamidine isethionate, 0.02% gutt polyhexamethyline biguanide, and laser In Situ keratomileusis (LASIK) for Myopia Improved vision blurriness and nearsightedness Lim and Wei (2004) [78]
Malaysian male 28 Corneal scraping Microscopy and culture AK Construction worker eye washed with water from open tank after sand and dust strucked in the eye Topical Propamidine isethionate, Chlorhexidine 0.02% and fortified Gentamycin Improved vision blurriness but loss of follow-up Kamel et al. (2005) [26]
Chinese male 24 Corneal scraping Microscopy and culture AK Non-disposable soft contact lens wearer and no contact lens when swim in lake/pool Before diagnosis: gutt spersadexoline; After diagnosis: 0.1% gutt propamidine isethionate, and gutt tobramycin Stromal scar Cheng et al. (2000) [79]
Malay male 26 Corneal scraping Microscopy and culture AK Non-disposable soft contact lens wearer Before diagnosis: tetracycline ointment and neosporin eyedrops; After diagnosis: 0.1% gutt propamidine isethionate Stromal scar Cheng et al. (2000) [79]
Thai female 58 Corneal scraping Microscopy, culture and mtDNA-RFLP AK Left eye injured by straw fragment and dirt cleaned off from her face using water in a jar near her home after digging in the garden on the outskirts Before diagnosis: antimicrobial eye drops and ointment, 1% trifluorothymidine eye drops and acyclovir eye ointment; After diagnosis: ketoconazole eye drops, neosporin, polymyxin, neomycin, gramicidin, propamidine isethionate eye drops, dibromopropamidine isethionate eye ointment, and transplantation. Recurrence necessitating evisceration Jongwutiwes et al. (2000) [80]
Thai male 30 Corneal scraping Microscopy, culture, and mtDNA-RFLP AK Splashing fish pond water to left eye injured by tiny piece of bamboo Before diagnosis: miconazole and neosporin eye drops; After diagnosis: propamidine isethionate eye drops, and dibromopropamidine isethionate eye ointment Vision blurriness Jongwutiwes et al. (2000) [80]
Thai female 57 Corneal scraping Microscopy, culture, indirect immunofluorescence testing, and isoenzyme analysis AK Pond water for washing Before diagnosis: spersapolymyxin eyedrops, cefazolin and gentamicin subconjunctival injection, topical neomycin sulfate, polymyxin B, and gramicidin; After diagnosis: 0.006% chlorhexidine hydrochloride with antidlaucoma for recurrence Improved vision blurriness with cataract Kosrirukvongs et al. (1999) [81]
Thai male 36 Corneal scraping Microscopy, culture, indirect immunofluorescence testing, and isoenzyme analysis AK Dust Before diagnosis: topical neomycin sulfate, polymyxin B, and gramicidin; After diagnosis: 0.006% chlorhexidine solution Loss of follow-up but no recurrence Kosrirukvongs et al. (1990) [81]
Thai female 33 Corneal scraping Microscopy, culture, indirect immunofluorescence testing, and isoenzyme analysis AK Daily-wear soft contact lenses Before diagnosis: fortified cefazolin, gentamicin, neomycin, topical tobramycin, topical neomycin sulfate, polymyxin B, and gramicidin; After diagnosis: 0.006% chlorhexidine solution Improved vision blurriness Kosrirukvongs et al. (1990) [81]
Thai male 74 Corneal scraping Microscopy, culture, indirect immunofluorescence testing, and isoenzyme analysis AK Plant root exposure Before diagnosis: antibiotics and plant root, topical neomycin sulfate, polymyxin B, and gramicidin; After diagnosis: 0.006% chlorhexidine solution and 1% topical clotrimazole eyedrops Note: non-compliance Enucleation Kosrirukvongs et al. (1990) [81]
Thai female 65 Corneal scraping Microscopy, culture, indirect immunofluorescence testing, and isoenzyme analysis AK Unknown Before diagnosis: topical neomycin sulfate, polymyxin B, and gramicidin; After diagnosis: cefazolin and gentamicin eye drops for P. aeruginosa as well as chlorhexidine for Acanthamoeba sp. Vision blurriness with cataract Kosrirukvongs et al. (1990) [81]
Malaysian female 40 Corneal scraping Microscopy AK with P. auruginosa and E. coli Contact lens wearer Before diagnosis: Zovirax® After diagnosis: gentamycin and homatropin eye drops, neosporin, miconazole eyedrops and Brolene® (0.1% Propamidine isethionate) - Kamel and Norazah (1995) [82]
Thai female 26 Brain autopsy Microscopy and indirect immunofluorescence test GAE Worker - Death Sangruchi et al. (1994) [83]
Thai male 20 Brain autopsy Microscopy and indirect immunofluorescence test GAE Farmer - Death Sangruchi et al. (1994) [83]
Thai female 42 Biopsy Radiography and microscopy Proliferated gastric ulcer with gastric acanthamoebiasis and sepsis from operative site with E. coli and K. pneumoniae Immunocompetent patients Venesection and rapid fluid replacement, antibiotics, gastrojejunostomy, and parenteral ampicillin, gentamicin, and metronidazole Death Thamprasert et al. (1993) [84]

AK, Acanthamoeba keratitis; GAE, Granulomatous amoebic encephalitis; -, Not mentioned in the published paper.

Anti-Acanthamoeba agents and nanoparticles in ASEAN studies

Anti-Acanthamoeba agents Nanotechnology Anti-Acanthamoeba activity against References
Cysts Trophozoites
Chemicals
 Cyclic samarium complexes [Sm(Pic)2(18C6)] (Pic) - - IC50=6.5 μg/ml against Acanthamoeba keratitis isolate Kusrini et al. (2018; Indonesia) [85]
 Acyclic samarium complexes [Sm(Pic)2(18C6)] (Pic) - - IC50=0.7 μg/ml against Acanthamoeba keratitis isolate Kusrini et al. (2018; Indonesia) [85]
 Terbium complex [Tb(NO3)3(OH2)3](18C6) - - IC50=7 μg/ml against Acanthamoeba keratitis isolate Kusrini et al. (2016; Indonesia) [86]
 Tb(NO3)3.6H2O in CH3CN - - IC50=2.6 μg/ml against Acanthamoeba keratitis isolate Kusrini et al. (2016; Indonesia) [86]
 18C6 in CH3CN - - IC50=1.2 μg/ml against Acanthamoeba keratitis isolate Kusrini et al. (2016; Indonesia) [86]
 Phosphanegold (I) thiolates - - No effect on viability, growth, cellular differentiation, and extracellular proteolytic activities against A. castellanii (ATCC50492) Siddiqui et al. (2017; Malaysia) [87]
 3% DMSO - Encystation induction and excystation inhibition against A. castellanii (ATCC50492) - Siddiqui et al. (2016; Malaysia) [88]
 Carbonyl Thiourea derivatives - - IC50=2.39–8.77 μg/ml against A. castellanii (CCAP 1501/2A) and 3.74–9.30 μg/ml against A. polyphaga (CCAP 1501/3A). Ibrahim et al. (2014; Malaysia) [89]
 Commercial fusaric acid - - IC50=0.33, 0.33, 0.66 μM against Acanthamoeba keratitis isolate and 2 soil isolates, respectively Boonman et al. (2012; Thailand) [90]
 Betadine® solution - MCC=0.04% dilution after 24 hr against Acanthamoeba keratitis isolate - Roongruangchai et al. (2011; Thailand) [91]
Virkon® solution - MCC=0.25% dilution after 24 hr against Acanthamoeba keratitis isolate - Roongruangchai et al. (2010; Thailand) [92]
Plant products
 Hesperidin, commercial flavonoid from Citrus sp. Silver nanoparticles stabilized by gum acacia Encystation and excystation inhibition against A. castellanii (ATCC 50492) 100% abolished amoeba viability of 5×105 A. castellanii (ATCC 50492) at 50μg/ml Anwar et al. (2019; Malaysia) [93]
 Naringin, commercial flavonoid, from Citrus sp. Gold nanoparticles stabilized by gum tragacanth Encystation and excystation inhibition against A. castellanii (ATCC 50492) Significanly abolished amoeba viability of 5×105 A. castellanii (ATCC 50492) at 50μg/ml Anwar et al. (2019; Malaysia) [93]
 Periglaucine A from Pericampylus glaucus Poly (DL-lactide-co-glycolide) CC50/IC50=100 against A. triangularis from environmental water sample CC50/IC50=25 against A. triangularis from environmental water sample Mahboob et al. (2018; Malaysia) [94]
 Betulinic acid from Pericampylus glaucus Poly (DL-lactide-co-glycolide) CC50/IC50=10 against A. triangularis from environmental water sample CC50/IC50=5 against A. triangularis from environmental water sample Mahboob et al. (2018; Malaysia) [94]
 Periglaucine A from Pericampylus glaucus - CC50/IC50=8.5 against A. triangularis from environmental water sample CC50/IC50=170 against A. triangularis from environmental water sample Mahboob et al. (2017; Malaysia) [95]
 Betulinic acid from Pericampylus glaucus - CC50/IC50=3.75 against A. triangularis from environmental water sample CC50/IC50=1.5 against A. triangularis from environmental water sample Mahboob et al. (2017; Malaysia) [95]
 Cinnamic acid from Cinnamomum cassia Gold nanopaticles Encystation inhibition against A. castellanii (ATCC 50492) Significantly enhanced anti-Acanthamoeba activity against A. castellanii (ATCC 50492) when compared with cinnamic acid alone Anwar et al. (2018; Malaysia) [96]
 Ethyl acetate, water, butanol fractions from Lonicera japonica - - Significant anti-Acanthamoeba effect against environmental A. triangularis trophozoites by ethyl acetate (most potent fraction) and cyst:trophozoites ratio reduction by commercial chlorogenic acid (major constituent in L. japonica) Mahboob et al. (2016; Malaysia) [97]
 Pouzolzia indica methanolic extract fraction 2 - MCC=1: 4 dilution after 24 hr against Acanthamoeba keratitis isolate - Roongruangchai et al. (2011; Thailand) [91]
 Pouzolzia indica methanolic extract fraction 3 - MCC=1: 8 dilution after 24 hr against Acanthamoeba keratitis isolate - Roongruangchai et al. (2010; Thailand) [92]
Microorganism products
 Supernatants from bacteria isolated from cockroach gut: Serratia marcescens and Escherichia coli from Madagascar cockroach; two Klebsiella spp., Citrobacter sp., Bacillus sp., Streptococcus sp. from Dubia cockroach - - Significant anti-Acanthamoeba effect against A. castellanii (ATCC 50492) Akbar et al. (2018; Malaysia) [98]
 Effective microorganisms (EM™) - Undiluted, 1:2, 1:4, 1:6 dilution of EM resulted in lower than 40% viable cysts - Sampaotong et al. (2016; Thailand) [99]
 Fusaric acid from Fusarium fujikuroi species complex Tlau3 isolated from Thunbergia laurifolia - - IC50=0.31 μm against Acanthamoeba keratitis isolate Boonman et al. (2012; Thailand) [90]
 Dehydrofuseric acid from Fusarium fujikuroi species complex Tlau3 isolated from Thunbergia laurifolia - - IC50=0.34 μm against Acanthamoeba keratitis isolate Boonman et al. (2012; Thailand) [90]
Drugs
 Nystatin, Fluconazole, and Amphotericin B Gold nanoparticles - Enhanced anti-Acanthamoeba activity at 10 μM (Amphotericin B>Fluconazole>Nystatin) against A. castellanii (ATCC 50492) Anwar et al. (2019; Malaysia) [100]
 Nystatin, Fluconazole, and Amphotericin B Silver nanoparticles - Enhanced anti-Acanthamoeba activity at 10 μM (Amphotericin B and Nystatin but not Fluconazole) against A. castellanii (ATCC 50492) Anwar et al. (2018; Malaysia) [101]
 Diazepam (Valium), Phenobarbitone (Luminal), and Phenytoin (Dilantin) And their silver nanoparticles Anti-Encystation activity (Diazepam and Phenobarbitone activity enhanced with silver nanoparticles) and anti-cyst activity (Phenobarbitone and Phenytoin activity enhanced with silver nanoparticles) against A. castellanii (ATCC 50492) Anti-Acanthamoeba activity observed and enhanced activity with silver nanoparticles against A. castellanii (ATCC 50492) Anwar et al. (2018; Malaysia) [102]
 Diclofenac sodium and Indomethacin (NSAIDs) - Encystation inhibition of A. castellanii (ATCC 50492) Growth affected but not viability of A. castellanii (ATCC 50492) Siddiqui et al. (2016; Malaysia) [103]
Acetaminophen (NSAIDs) - No effects on encystation inhibition of A. castellanii (ATCC 50492) No effects on growth of A. castellanii (ATCC 50492) Siddiqui et al. (2016; Malaysia) [103]
 Bortezomib (proteasome inhibitor) - Encystation inhibition against A. castellanii (ATCC 50492) Static effect on growth but not viability of A. castellanii (ATCC 50492) Siddiqui et al. (2016; Malaysia) [104]
 Lactacystin and active form as clasto-lactacystin β-lactone (proteasome inhibitors) - Encystation inhibition and excystation inhibition against A. castellanii (ATCC 50492) No effects on growth and viability of A. castellanii (ATCC 50492) Siddiqui et al. (2016; Malaysia) [104]
 Artesunate (Antimalaria) - Presence of cytostatic effect on Acanthamoeba polyphaga-like amoebae were isolated from natural water courses at concentrations of 500–700 μg/ml Dose-dependent growth inhibition (5–700 μg/ml) against Acanthamoeba polyphaga-like amoebae were isolated from natural water courses Nacapunchai et al. (2003; Thailand) [105]
 Metronidazole - No effects (5–1,000 μg/ml) No effects (5–1,000 μg/ml) Nacapunchai et al. (2003; Thailand) [105]
Animal products
 Crocodile (Crocodylus palustris) serum - - Anti-Acanthamoeba activity against A. castellanii (ATCC 50492) Siddiqui et al. (2017; Malaysia) [106]
 Sea sponge crude methanol extracts (Aaptos aaptos) from different localities - - IC50=0.615–0.876 μg/ml against clinical A. castellanii Nakisah et al. (2012; Malaysia) [107]

IC, Inhibition concentration; CC, Cytotoxicity concentration; MCC, Minimal cystcidal concentation; -, Not mentioned in the published paper.

Table 1 Distribution of environmental Acanthamoeba spp. in Southeast Asia

ND, Not detected; NF, Not found.

Table 2 Examples of Acanthamoeba infection cases in Southeast Asia

AK, Acanthamoeba keratitis; GAE, Granulomatous amoebic encephalitis; -, Not mentioned in the published paper.

Table 3 Anti-Acanthamoeba agents and nanoparticles in ASEAN studies

IC, Inhibition concentration; CC, Cytotoxicity concentration; MCC, Minimal cystcidal concentation; -, Not mentioned in the published paper.

Table 1

Table 2

Table 3

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