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Original Article

Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite

Eui-Sun Son1, Tong Soo Kim2, Ho-Woo Nam1
The Korean Journal of Parasitology 2001;39(2):171-176.
Published online: June 30, 2001

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• Received: April 3, 2001   • Accepted: May 16, 2001

copyright © 2001 by The Korean Society for Parasitology

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Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite
Korean J Parasitol. 2001;39(2):171-176.   Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.171
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Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite
Korean J Parasitol. 2001;39(2):171-176.   Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.171
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Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite
Image Image
Fig. 1 PCR-amplified coding gene fragments of Plasmodium vivax separated in 1.2% agarose gel.
Fig. 2 Confirmation of GST fusion proteins by western blot with anti-GST antibody.

Fig. 1

Fig. 2

Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite
Genes Name of primers Sequences Annealing Temp.(°C) References
CSP-1 CSPF 5′-cac gta gga caa agt gct agc-3′ 52 Arnot et al., 1990
CSPR 5′-tac act gca tgg agt cca ttc-3′
MSP-1 MSPF 5′-cta cta ctt gat ggt cct caa-3′ 56 Del Portillo et al., 1991
MSPR 5′-ttg tga cat gcg taa gcg gat-3′
AMA-1 AMAF 5′-atg aat aaa ata tac tac ata-3′ 55 Rogers et al., 1999
AMAR 5′-ata ctg agt tgg ttg atc tga-3′
SERA SERAF 5′-aga ggg gtg cac aac atg tgc-3′ 55 Gor et al., 1998
SERAR 5′-tca cac ata gca gaa gta gca-3′
EXP-1 EXPF 5′-gaa aaa aca aac aaa gaa-3′ 54 Simmons et al., 1987
EXPR 5′-tac agc tgt tta gtg ttc agg-3′
Antigen P. vivax P. falciparum Pv/Pf mixed Negative
CSP-1 128/160 (80.0) 8/13 (61.5) 1/9 (11.1) 0/20
MSP-1 102/160 (63.8) 8/13 (61.5) 6/9 (66.7) 0/20
AMA-1 128/160 (80.0) 8/13 (61.5) 2/9 (22.2) 0/20
SERA 115/160 (71.9) 6/13 (46.2) 4/9 (44.4) 0/20
EXP-1 89/160 (55.9) 12/13 (92.3) 9/9 (100.0) 0/20
Total 147/160 (91.9) 12/13 (92.3) 9/9 (100.0) 0/20
1a) 2 3 4 5
Ag NPb)(%) Ag NP (%) Ag NP (%) Ag NP (%) Ag NP (%)
C 128 (80.0) CM 138 (86.3) CMA 140 (87.5) CMAS 143 (89.4) CMASE 147 (91.9)
M 102 (63.8) CA 132 (82.5) CMS 142 (88.8) CMAE 145 (90.6)
A 128 (80.0) CS 135 (84.4) CME 144 (90.0) MASE 146 (91.3)
S 115 (71.9) CE 138 (86.3) CAS 137 (85.6)
E 89 (55.6) MA 138 (86.3) CAE 141 (88.1)
MS 130 (81.3) CSE 143 (89.4)
ME 122 (76.3) MAS 142 (88.8)
AS 136 (85.0) MAE 145 (90.6)
AE 140 (87.5) ASE 143 (89.4)
SE 134 (83.8)
Table 1. Primer sets designed for the amplification of antigenic domains of each protein
Table 2. Seroreactivity of each antigen on the blood smear positive sera
Table 3. Positive detection of vivax malaria by the number of antigens used in western blot

No. of Ag used

NP: number of positive among 160 tested

C, CSP-1; M, MSP-1; A, AMA-1; S, SERA; and E, EXP-1

Table 1.

Table 2.

Table 3.

PHD : Parasites, Hosts and Diseases

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