Different RAPD patterns between <i>Metagonimus yokogawai</i> and <i>Metagonimus</i> Miyata type

Brief Communication


Korean J Parasitol. 1997 Dec;35(4):295-298. English. Published online Dec 20, 1997. http://dx.doi.org/10.3347/kjp.1997.35.4.295
Copyright © 1997 by The Korean Society for Parasitology


Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type
J R Yu,^*¹J S Chung,¹ and J Y Chai²
¹Department of Parasitology, College of Medicine, Kon-Kuk University, Chungju 380-701, Korea.
^*Corresponding author (Email: yu@kcucc.konkuk.ac.kr)

Received August 25, 1997; Accepted November 10, 1997.
Abstract
Genomic DNA from Metagonimus yokogawai and Metagonimus Miyata type was amplified by polymerase chain reaction based on the random amplification of polymorphic DNA (RAPD) technique. Eight random 10-mer oligonucleotide primers (OPA-02, 5-TGCCGAGCTG-3; OPA-09, 5-GGGTAACGCC-3; OPA-10, 5-GTGATCGCAG-3; OPA-11, 5-CAATCGCCGT-3; OPA-13, 5-CAGCACCCAC-3; OPA-17, 5-GACCGCTTGT-3; OPA-19, 5-CAAACGTCGG-3; OPA-20, 5-GTTGCGATCC-3) with a G + C content of 60-70% (Kit A, Operon Technologies Inc., California, USA) could produce distinguishable banding patterns between the two Metagonimus species. From the results of this study, it was suggested that Metagonimus Miyata type has a different DNA sequence from M. Yokogawai.

Figures


	Fig. 1 Silver stained polyacrylamide gel showing RAPD profiles of Metagonimus species. OPA, primer number; m, Metagonimus Milyata type; y, M. yokogawai; M₁, M₂, marker.


	Fig. 2 Silver stained polyacrylamide gel showing RAPD profiles of Metagonimus species. OPA, primer number; m, Metagonimus miyata type; y, M. yokogawai; M₁, M₂, marker.

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