Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81
Warning: fopen(upload/ip_log/ip_log_2024-04.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Chronologic change of serum IgG antibody response in chickens reinfected with Cryptosporidium baileyi
Chronologic change of serum IgG antibody response in chickens reinfected with Cryptosporidium baileyi
Jae Ku Rhee,*Hyeon Cheol Kim and Bae Keun Park
Bio-Safety Research Institute, Chonbuk National University, Chonju 561-756, Korea.
Received September 14, 1996; Accepted November 09, 1996.
Abstract
Eight 2-day-old SPF chickens were each inoculated orally with a single dose of 5×105 oocysts of Cryptosporidium baileyi, and immunoglobulin G (IgG) antibody responses were chronologically measured by indirect immunofluorescent antibody (IFA) assay. Anti-C. baileyi IgG antibody levels remained high(1:106.67 to 1:512.00) for at least 4 months with 330 days of a detectable period. Ten days after the negative conversion, each chicken was re-challenged with 1×107 oocysts of the same species. Subsequent infection in 340-day-old individuals caused sudden elevated IgG antibody levels and the titer peaked on day 28 postchallenge inoculation(PCI), at 1:1,024 with a 65 days of detection period. Chickens in primary infection showed oocyst shedding profiles, but did not exhibit any oocyst shedding before or after experimental reinfection.
Figures
Fig. 1 Serum IgG antibody levels in the primary and secondary antibody responses to Cryprosporidium baileyi in chickens reinfected with oocysts.
References
1.
Arrowood MJ, Sterling CR. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol 1987;73(2):314–319.
2.
Campbell PN, Current WL. Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections. J Clin Microbiol 1983;18(1):165–169.
3.
Current WL, Upton SJ, Haynes TB. The life cycle of Cryptosporidium baileyi n sp (Apicomplexa, Cryptosporidiidae) infecting chickens. J Protozool 1986;33(2):289–296.
4.
Current WL, Snyder DB. Development of and serologic evaluation of acquired immunity to Cryptosporidium baileyi by broiler chickens. Poult Sci 1988;67(5):720–729.
5.
Ditrich O, Palkovic L, Sterba J, Prokopic J, Loudova J, Giboda M. The first finding of Cryptosporidium baileyi in man. Parasitol Res 1991;77(1):44–47.
6.
Hatkin J, Giambrone JJ, Blagburn BL. Correlation of circulating antibody and cellular immunity with resistance against Cryptosporidium baileyi in broiler chickens. Avian Dis 1993;37(3):800–804.
9.
Naciri M, Mancassola R, Reperant JM, Yvore P. Analysis of humoral immune response in chickens after inoculation with Cryptosporidium baileyi or Cryptosporidium parvum. Avian Dis 1994;38(4):832–838.
10.
Ortega-Mora LM, Troncoso JM, Rojo-Vazquez FA, Gomez-Bautista M. Serum antibody response in lambs naturally and experimentally infected with Cryptosporidium parvum. Vet Parasitol 1993;50(1-2):45–54.
11.
Ohsawa K. J Osaka City Med Cen 1993;42(1):17–32.
13.
Rhee JK, Seu YS, Park BK. [Isolation and identification of Cryptosporidium from various animals in Korea III Identification of Cryptosporidium baileyi from Korean chicken]. Korean J Parasitol 1991;29(4):315–324.
14.
Rhee JK, Jang BG, Park BK. Oocyst production and immunogenicity of Cryptosporidium baileyi in chickens and mallards. Korean J Parasitol 1995;33(1):45–54.
15.
Riggs MW, Perryman LE. Infectivity and neutralization of Cryptosporidium parvum sporozoites. Infect Immun 1987;55(9):2081–2087.
16.
Ungar BL, Mulligan M, Nutman TB. Serologic evidence of Cryptosporidium infection in US volunteers before and during Peace Corps service in Africa. Arch Intern Med 1989;149(4):894–897.
17.
Ungar BL, Kao TC, Burris JA, Finkelman FD. Cryptosporidium infection in an adult mouse model Independent roles for IFN-gamma and CD4+ T lymphocytes in protective immunity. J Immunol 1991;147(3):1014–1022.
PDF Links
Full text via DOI
Download Citation 
