Three-week-old ICR SPF mice were orally inoculated with one of 5 doses ranging from 2 x 10(2) to 2 x 10(6) oocysts of Cryptosporidium muris (strain MCR) per mouse. Oocyst inoculation was directly proportional to the amount of oocysts shed and was inversely proportional to the period required for peak oocyst production and to the prepatent period. Peak oocyst production occurred between fifteen and thirty-one days with a patent period from 61 to 64 days.
Three days after all mice stopped shedding oocysts, they were orally challenged with a single dose of 2 x 10(6) oocysts of the same species. Marked seroconversion for IgG antibody accompanied recovery from mice inoculated with 5 x 10(5) oocysts. Mice administered with carrageenan excreted a small number of oocysts for 49.0 days on the average after challenge inoculation (ACI) and control mice for 14.2 days in a dose-independent fashion. Just before challenge infection, phagocytic activity of peritoneal macrophages (M phi) and the number of peripheral M phi were dramatically decreased. Mild challenge infection implies that the immunogenicity of C. muris (strain MCR) is very strong, despite M phi blocker carrageenan administration.
Citations
Citations to this article as recorded by
Diversity of Cryptosporidium spp. in Apodemus spp. in Europe Šárka Čondlová, Michaela Horčičková, Nikola Havrdová, Bohumil Sak, Lenka Hlásková, Agnieszka Perec-Matysiak, Marta Kicia, John McEvoy, Martin Kváč European Journal of Protistology.2019; 69: 1. CrossRef
Cryptosporidium proliferans n. sp. (Apicomplexa: Cryptosporidiidae): Molecular and Biological Evidence of Cryptic Species within Gastric Cryptosporidium of Mammals Martin Kváč, Nikola Havrdová, Lenka Hlásková, Tereza Daňková, Jiří Kanděra, Jana Ježková, Jiří Vítovec, Bohumil Sak, Ynes Ortega, Lihua Xiao, David Modrý, Jeba Rose Jennifer Jesudoss Chelladurai, Veronika Prantlová, John McEvoy, Herbert B. Tanowitz PLOS ONE.2016; 11(1): e0147090. CrossRef
The Lesser Egyptian Gerbil (Gerbillus gerbillus) is a suitable host for the long-term propagation of Cryptosporidium andersoni Martin Kváč, Zuzana Ondráčková, Dana Květoňová, John McEvoy, Jiří Vítovec, Michael Rost, Bohumil Sak Experimental Parasitology.2013; 134(4): 438. CrossRef
Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice Marie Jalovecká, Bohumil Sak, Martin Kváč, Dana Květoňová, Zuzana Kučerová, Jiří Salát Parasitology Research.2010; 106(5): 1159. CrossRef
Infectivity and pathogenicity of Cryptosporidium andersoni to a novel host, southern multimammate mouse (Mastomys coucha) Martin Kváč, Zuzana Ondráčková, Dana Květoňová, Bohumil Sak, Jiří Vítovec Veterinary Parasitology.2007; 143(3-4): 229. CrossRef
Variation in Cryptosporidium: towards a taxonomic revision of the genus Una M Morgan, Lihua Xiao, Ronald Fayer, Altaf A Lal, R.C.Andrew Thompson International Journal for Parasitology.1999; 29(11): 1733. CrossRef
Age-dependent resistance to Cryptosporidium muris (strain MCR) infection in golden hamsters and mice Jae Ku Rhee, Wang Su So, Hyeon Cheol Kim The Korean Journal of Parasitology.1999; 37(1): 33. CrossRef
The pathogenesis of experimental infections of Cryptosporidium muris (strain RN 66) in outbred nude mice M.A. Taylor, R.N. Marshall, J.A. Green, J. Catchpole Veterinary Parasitology.1999; 86(1): 41. CrossRef
Infection kinetics and developmental biology of Cryptosporidium muris (strain MCR) in Korean native kids and Corriedale lambs J K Rhee, H C Kim, G S Eun The Korean Journal of Parasitology.1998; 36(3): 171. CrossRef
Effects of Cryptosporidium muris (strain MCR) infection on gastric mucosal mast cells in mice J K Rhee, C G Surl, H C Kim The Korean Journal of Parasitology.1997; 35(4): 245. CrossRef
Survival of Cryptosporidium muris (strain MCR) oocysts under cryopreservation J K Rhee, B K Park The Korean Journal of Parasitology.1996; 34(2): 155. CrossRef
