Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to <i>Acanthamoeba polyphaga</i>

, Kong, H H; , Park, J H; , Chung, D I

doi:1995.33.4.331

Korean J Parasito > Volume 33(4):1995 > Article

Original Article


Korean J Parasitol. 1995 Dec;33(4):331-340. English. Published online Dec 20, 1995. http://dx.doi.org/10.3347/kjp.1995.33.4.331
Copyright © 1995 by The Korean Society for Parasitology


Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga
Hyun-Hee Kong,Jun-Hyung Park and Dong-Il Chung^*
Department of Parasitology, Kyungpook National University School of Medicine, Taegu 700-422, Korea.

Received September 12, 1995; Accepted November 17, 1995.
Abstract
Interstrain polymorphisms of isoenzyme profiles and mitochondrial (Mt) DNA fingerprints were observed among seven strains of Acanthamoeba isolated from different sources and morphologically assigned to A. polyphaga. Mt DNA fingerprints by eight restriction endonucleases (Bgl II, Sca I, Cla I, EcoR I, Xba I, Kpn I, Sal I, and Sst I) revealed considerable interstrain polymorphisms. Isoenzyme profiles revealed considerable interstrain polymorphisms for acid phosphatase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase while those for glucose phosphate isomerase, leucine aminopeptidase, and malate dehydrogenase showed similarity. Despite of the interstrain polymorphisms, the isoenzyme profiles and Mt DNA fingerprints of the strain Ap were found to be identical with those of the strain Jones. Mt DNA fingerprinting was found to be highly applicable for the strain identification, characterization, and differentiation.

Figures


	Fig. 1 Photomicrographs of the trophozoites and the typical cyst of A. polyphaga. A. Trophozoites a. Differential interference contrast, characteristic acanthpodia (arrows); b. Trichrome strained, bull's eye shaped nucleus (arrow head); B. Cyst of strain Jones. The ectocyst is relatively thin and close to the endocyst. Bars indicate 10 µm.


	Fig. 2 Isoenzymes of Acanthamoeba seven strains separated by polyacrylamide gel isoelectric focusing in pH gradient 3-10. A. Acid phosphatase, B. Glucose phosphate Isomerase; C. Leucine aminopeptidase; D. Lactate dehydrogenase; E. Malate dehydrogenase; F. Glucose-6-phosphate dehydrogenase. Numbers above lanes refer to the strains listed in Table 2.


	Fig. 3 Total proteines of Acanthamoeba separated by polyacrylamide gel isoelectric focusing in pH gradient 3-7. Numbers above lanes refer to the strains listed in Table 2.


	Fig. 4 Agarose gel electrophoretic fingerprints of mitochondrial DNA from seven strains assigned to A. polyphaga. A. Cla I digests; B. Sca I digests; C. Bgl II digests; D. Hpa I digests; E. EcoR I digests; F. Sst I digests; G. Xba I digests; H. Sal I digests. Numbers above lanes refer to the strains listed in Table 2. Size marker is Hind III digested λ phage DNA (M).

Tables


	Table 1 Developmental conditions of enzymes tested (Final conc./100 ml)


	Table 2 Morphology of Acanthamoeba polyphaga strains analysed in this study

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