Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Chronological observation on intestinal histopathology and intraepithelial lymphocytes in the intestine of rats infected with Metagonimus yokogawai
| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasito Search

CLOSE

Korean J Parasito > Volume 32(4):1994 > Article

Original Article
Korean J Parasitol. 1994 Dec;32(4):215-221. Korean.
Published online Dec 20, 1994.  http://dx.doi.org/10.3347/kjp.1994.32.4.215
Copyright © 1994 by The Korean Society for Parasitology
Chronological observation on intestinal histopathology and intraepithelial lymphocytes in the intestine of rats infected with Metagonimus yokogawai
Jong-Yi Chai,*1Tae-Young Yun,1Jin Kim,2Sun Huh,3Min-Ho Choi,1 and Soon-Hyung Lee1
1Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received September 06, 1994; Accepted November 28, 1994.

Abstract

The relationship between the intestinal histopathology and number and position of intraepithelial lymphocytes (IEL) was observed chronologically in the small intestine of rats experimentally infected with Metagonimus yokogawai. Fifteen Sprague-Dawley rats were orally infected each with 3,000 metacecariae, and 3 were kept uninfected for controls. Three rats each were sacrificed on the day 5, 10, 15, 24 and 70 post-infection (PI) and samples of the small intestine, 5 cm, 10 cm, 20 cm and 70 cm posterior to the pylorus were taken. The samples were processed routinely and stained with Giemsa. The intestinal histopathology was severe during the day 5-15 PI and characterized by villous atrophy, crypt hyperplasia, and decrease of villus/crypt height ratio. After the day 24 PI, the intestinal lesions showed some tendency of recovery. The number of IEL increased at the early stage of infection, but decreased thereafter to a lower level than that of controls, with progression of the pathological changes. Then, the IEL number began to increase again after the day 24 PI. In control rats, the great majority of the IEL were located at the basal region of the epithelium. During the early stage of infection, however, a considerable proportion of IEL was found to have moved to the intermediate or apical region of the epithelium. From the above results, it is suggested that the change of IEL number and position during the course of M. yokogawai infection should be closely related to the progression and recovery of the intestinal histopathology.

Figures


Fig. 1
Chronological changes in the number of intraepithelial lymphocytes (IEL) at 4 different portions of the small intestine of rats (S-D) infected with M. yokogawai. In all of the 4 portions the IEL number increased rapidly at 5 days post-infection (PI), but decreased to a lower level than day 0 (control) during 10-24 days PI, and increased again up to slightly higher level than day 0. Values represent the mean IEL count among 500 epithelial cells in 3 rats, S.D. never exceeded 1/3 of the mean value.


Fig. 2
Chronological changes in the number of IEL (average value of 4 portions of the small intestine; Fig. 1) in comparison with the V/C ratio (mean value; Table 1) in rats infected with M. yokogawai. Note that the IEL number and V/C ratio inversely correlate during the early stages (5-15 days) of infection.


Figs. 3-8
Fig. 3. An epithelial layer of the small intestine of a control rat. All intraepithelial lymphocytes (IEL) are located at the basal region (arrows) of the epithelium (× 1,200). Fig. 4. Three IEL (arrows) in a M. yokogawai-infected rat, 5 days post-infection. Each of the IEL is located at apical, intermediate and basal regions of the epithelium (× 1,200). Fig. 5. Jejunum of a 5-day Infection rat. Many IEL (arrows) are located near the intermediate region of the epithelium (× 480). Fig. 6. Two typical IEL (arrows) in the jejunum of a 5-day infection rat. They are located near the perinuclear region of the epithelial cells. The nuclei of IEL are round or oval (× 1,800). Fig. 7. Jejunum of a 5-day infection rat. The villi are severely atrophied, deformed, flat, and fused, and the crypts are hyperplastic. Decreased V/C ratio and increased number of goblet cells are seen (× 120). Fig. 8. Jejunum of a 10-day infection rat, showing two sectioned worms of M. yokogawai. The worms are sucking the villi with their oral suckers. Some parts of the neighboring villi were destroyed, and the villi show increased number of goblet cells (× 120).

Tables


Table 1
V/C ratios in the small intestine of rats infected with M. yokogawai by post-infection days


Table 2
Location of IEL within the epithelium of small intestinal villi in rats infected with M. yokogawai

References
1. Carroll SM, Mayrhofer G, Dawkins HJ, Grove DI. Kinetics of intestinal lamina propria mast cells, globule leucocytes, intraepithelial lymphocytes, goblet cells and eosinophils in murine strongyloidiasis. Int Arch Allergy Appl Immunol 1984;74(4):311–317.
  
2. Chai JY. Seoul J Med 1979;20(2):104–112.
3. Chai JY, Lillehoj HS. Isolation and functional characterization of chicken intestinal intra-epithelial lymphocytes showing natural killer cell activity against tumour target cells. Immunology 1988;63(1):111–117.
 
4. Darlington D, Rogers AW. Epithelial lymphocytes in the small intestine of the mouse. J Anat 1966;100(Pt 4):813–830.
 
5. Ferguson A. Intraepithelial lymphocytes of the small intestine. Gut 1977;18(11):921–937.
  
6. Ferguson A, McClure JP, Townley RR. Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea. Acta Paediatr Scand 1976;65(5):541–546.
  
7. Ferguson A, Murray D. Quantitation of intraepithelial lymphocytes in human jejunum. Gut 1971;12(12):988–994.
  
8. Hoste H, Kerboeuf D, Parodi AL. Trichostrongylus colubriformis: effects on villi and crypts along the whole small intestine in infected rabbits. Exp Parasitol 1988;67(1):39–46.
  
9. Kang SY, Cho SY, Chai JY, Lee JB, Jang DH. A Study On Intestinal Lesions Of Experimentally Reinfected Dogs With Metagonimus Yokogawai. Korean J Parasitol 1983;21(1):58–73.
 
10. Lee JB, Chi JG, Lee SK, Cho SY. Study On The Pathology Of Metagonimiasis In Experimentally Infected Cat Intestine. Korean J Parasitol 1981;19(2):109–129.
 
11. Lillehoj HS, Trout JM. CD8+ T cell-coccidia interactions. Parasitol Today 1994;10(1):10–14.
  
12. MacDonald TT, Ferguson A. Small intestinal epithelial cell kinetics and protozoal infection in mice. Gastroenterology 1978;74(3):496–500.
 
13. Otto HF. The interepithelial lymphocytes of the intestinum. Morphological observations and immunologic aspects of intestinal enteropathy. Curr Top Pathol 1973;57:81–121.
 
14. Owen RL, Nemanic PC, Stevens DP. Ultrastructural observations on giardiasis in a murine model. I. Intestinal distribution, attachment, and relationship to the immune system of Giardia muris. Gastroenterology 1979;76(4):757–769.
 
15. Upadhyay P, Ganguly NK, Walia BN, Mahajan RC. Kinetics of lymphocyte subpopulation in intestinal mucosa of protein deficient Giardia lamblia infected mice. Gut 1986;27(4):386–391.
  
16. Rothwell TL, Dineen JK. Cellular reactions in guinea-pigs following primary and challenge infection with Trichostrongylus colubriformis with special reference to the roles played by eosinophils and basophils in rejection of the parasite. Immunology 1972;22(5):733–745.
 
16. Ruitenberg EJ, Elgersma A, Kruizinga W, Leenstra F. Trichinella spiralis infection in congenitally athymic (nude) mice. Parasitological, serological and haematological studies with observations on intestinal pathology. Immunology 1977;33(4):581–587.
 
17. Symons LEA, Falrbairn D. Gastroenterology 1962;21:913–918.
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI