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Effects of cytokines in the activation of peritoneal macrophages from mice infected with Toxoplasma gondii
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Korean J Parasito > Volume 32(3):1994 > Article

Original Article
Korean J Parasitol. 1994 Sep;32(3):185-193. English.
Published online Sep 20, 1994.  http://dx.doi.org/10.3347/kjp.1994.32.3.185
Copyright © 1994 by The Korean Society for Parasitology
Effects of cytokines in the activation of peritoneal macrophages from mice infected with Toxoplasma gondii
Y H Lee,* and D W Shin
Department of Parasitology, College of Medicine, Chungnam National University, Taejon 301-131, Korea.
Received July 08, 1994; Accepted July 22, 1994.

Abstract

The present study was undertaken to assess the role of cytokines in the activation of peritoneal macrophages from Toxoplasma-infected mice. Peritoneal macrophages from Toxoplasma-infected mice (10 cysts of Beverley strain/mouse) were harvested 8 weeks after infection, and incubated with the mitogen-induced lymphokine, recombinant mouse interferon-γ (IFN-γ), recombinant mouse tumor necrosis factor-α (TNF-α) alone or in combination with IFN-γ (IFN-γ/TNF-α) for 24 hr at 37℃, 5% CO2. Macrophage activation was measured by the amount of H2O2 and NO2- production, and anti-Toxoplasma activities of macrophages. IFN-γ or IFN-γ/TNF-α-treated macrophages from Toxoplasma-infected mice revealed significantly higher H2O2 production than resident macrophages from Toxoplasma-infected mice. The production of NO2- by TNF-α-, IFN-γ- or IFN-γ/TNF-α-treated macrophages from Toxoplasma-infected mice were significantly higher than that by resident macrophages, whereas lymphokine-treated group produced similar amount as that produced by resident macrophages. Anti-Toxoplasma activities of cytokine-treated macrophages from Toxoplasma-infected mice were significantly higher than those of resident macrophages. IFN-γ-treated macrophages were significantly increased production of H2O2 and NO2-, and anti-Toxoplasma activities of macrophages between normal and Toxoplasma-infected mice, whereas the other cytokine-treated groups were not significant differences between them. These data suggested that IFN-γ was the only one of cytokines capable of significantly activating the peritoneal macrophages from Toxoplasma-infected mice.

Figures


Fig. 1
Production of hydrogen peroxide by cytokine-treated macrophages from normal and Toxoplasma-infected mice. The peritoneal macrophages were incubated 24 hours after treatment of cytokine. All results are mean ± standard error of 3 experiments. *Indicates values significantly different (P<0.05) from those for resident macrophages.


Fig. 2
Production of nitrite by cytokine-treated macrophages from normal and Toxoplasma-infected mice.


Fig. 3
Anti-Toxoplasma activities of cytokine-treated macrophages from normal and Toxoplasma-infected mice.

Tables


Table 1
Correlation coefficient form the production of hydrogen peroxide, nitrite, and anti-Toxoplasma activities of macrophages from normal and Toxoplasma-infected mice

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