Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients, and 20 normal control. All but one patient each in 3 nematodiases showed the antibody levels of negative range. The positively reacted patients were regarded as concomitant infections of sparganum because immunized or hyperinfected rabbit serum of the nematodes did not react crossly to the antigen.
