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Comparative study on the effectiveness of modified Kato's cellophane thick smear and Stoll's dilution egg counting technique for quantitative fecal examination of helminth eggs
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Korean J Parasito > Volume 30(2):1992 > Article

Original Article
Korean J Parasitol. 1992 Jun;30(2):141-145. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.2.141
Copyright © 1992 by The Korean Society for Parasitology
Comparative study on the effectiveness of modified Kato's cellophane thick smear and Stoll's dilution egg counting technique for quantitative fecal examination of helminth eggs
S J Hong,H C Woo,J H Han and H J Kim*
Department of Parasitology, College of Medicine, Gyeongsang National University, Chinju 660-280, Korea.
Abstract

A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E.C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T. in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were 47.86 × 100.87 logM.C.T.S. in A. lumbricoides, 41.69 × 100.82 logM.C.T.S. in T. trichiura and 63.10 × 100.85 logM.C.T.S. in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.

Tables


Table 1
Log10 values by modified Kato's cellophane thick smear (M.C.T.S.) and by Stoll's dilution egg counting techniques(D.E.C.T.)

References
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