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Parasit Host Dis > Volume 29(4):1991 > Article

Original Article
Korean J Parasitol. 1991 Dec;29(4):371-380. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.4.371
Copyright © 1991 by The Korean Society for Parasitology
Attempts to transfer immunity against Clonorchis sinensis in nude and DS mice
D W Choi,D I Chung and D H Kang
Department of Parasitology, Kyungpook National University, School of Medicine, Taegu 700-422, Korea.

The effects of peritoneal exudate cells (PEC) and sera of athymic nude and DS mice infected with Clonorchis sinensis metacercariae or sensitized by injection of metabolic products into footpad on transfer of immunity against the fluke to the syngeneic mice were studied.

There was no significant difference in eggs per gram pattern between the sensitized and control groups, and between nude and DS mice. However, the worm burdens were slightly greater in nude mice than in DS mice. Also, a few plaque forming cells were found in only DS mice given PEC and serum from Group II DS mice.

In the light of these results, it is likely that PEC and sera of nude or DS mice which are deficient, at least partially, in the cellular immune system are unable to transfer immunity against C. sinensis to syngeneic recipients.


Table 1
Experimental groups to study the effects of peritoneal exudate cells and sera on transfer of immunity against Clonorchis sinensis in nude and DS mice

Table 2
Proportion of viable cells per ml of peritoneal exudate cells in donor nude and DS mice by trypan blue exclusion

Table 3
Effect of sensitized peritoneal exudate cells and sera on Eggs per Gram of feces in recipient nude and DS mice after challenging infection

Table 4
Effect of peritoneal exudate cells and sera sensitized by oral infection or footpad injection of metabolic products of adult C. sinensis on worm burdens C. sinensis in recipient nude and DS mice

Table 5
Viable spleen cells and plaque forming cells in recipient mice after challenging infection

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