Isolation and identification of <i>Cryptosporidium</i> from various animals in Korea. III. Identification of <i>Cryptosporidium baileyi</i> from Korean chicken

, Rhee, J K; , Seu, Y S; , Park, B K

doi:1991.29.4.315

Korean J Parasito > Volume 29(4):1991 > Article

Original Article


Korean J Parasitol. 1991 Dec;29(4):315-324. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1991.29.4.315
Copyright © 1991 by The Korean Society for Parasitology


Isolation and identification of Cryptosporidium from various animals in Korea. III. Identification of Cryptosporidium baileyi from Korean chicken
J K Rhee,Y S Seu and B K Park
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju, Korea.


Abstract
Each of SPF chicken (Hi-Line strain, 2-day-old males) was inoculated with 2.5 or 5 × 10⁴ oocysts by stomach tube. The oocyst was the medium type of Cryptosporidium previously isolated from Korean chicken origin, and passed in 2-day-old SPF chicken. The patterns of oocyst discharge were monitored daily, and in order to observe the ultrastructure of the developmental stages, the bursa of Fabricius of the chicken was examined by transmission electron microscopy (TEM) on the 12th day postinoculation. The prepatent period for 8 chicken was 5.9 days postinoculation on the average, and the patent period was 12.9 days. The number of oocysts discharged per day for the chicken was reached peak on day 12 postinoculation on the average. A large number of oocysts was found in fecal samples obtained from inoculated chicken on days 8-14 postinoculation. The ultrastructural feature of almost every developmental stage of the medium type from chicken was very similar to that of Cryptosporidium previously isolated from mammalia including human and birds except for the attachment site of C. muris to the mucus cell from mammalia, but dimension of the oocysts from fecal samples of the medium type was different from those of C. meleagridis and mammalia origin. The above results reveal that the medium type of Cryptosporidium of Korean chicken origin is identified as Cryptosporidium baileyi.

Figures


	Fig. 1 Pattern of oocyst discharge in chicken inoculated with 2.5×10⁴Cryptosporidium oocysts(Korean chicken origin).

Figs. 2-3
Transmission electron micrographs showing the endogenous development of Cryptosporidium baileyi parasitizing the microvillous border of epithelial cells lining the plicae of the bursa of Fabricius.

Fig. 2. The parasites growing in the microvilli of epithelial cells lining the plicae of the bursa of Fabricius. Various developmental stages can be seen, ×9,200.

Fig. 3. Young trophozoite(YT) and meront(M) including merozoites(ME) and residual body(REB). Merozoites showing nucleus(N), rough endoplasmic reticulum(RER) and micronemes(MN), ×20,000.

Figs. 4-5
Transmission electron micrographs showing the endogenous development of Cryptosporidium baileyi parasitizing the microvillous border of epithelial cells lining the plicae of the bursa of Fabricius.

Fig. 4. Meront comprising merozoites(ME) and residual body(REB). Merozoites showing nucleus and rough endoplasmic reticulum(RER), ×23,000.

Fig. 5. Mature macrogamete showing amylopectin granules(AP), wall-forming bodies(WFB) and rough endoplasmic reticulum, ×20,000.

Figs. 6-7
Transmission electron micrographs showing the endogenous development of Cryptosporidium baileyi parasitizing the microvillous border of epithelial cells lining the plicae of the bursa of Fabricius.

Fig. 6. Microgamont containing several microgametes(MIG). Microgametes showing an expanded anterior part, microtubule(MT) and compact nucleus, ×27,000.

Fig. 7. Oocyst with oocyst wall(W), sporozoite(SP), residual body(REB) and rough endoplasmic reticulum(RER). Sporozoite showing nucleus(N) and micronemes(MN), ×18,000.

Figs. 8-10
Transmission electron micrographs showing the endogenous development of Cryptosporidium baileyi parasitizing the microvillous border of epithelial cells lining the plicae of the bursa of Fabricius.

Fig. 8. Oocyst showing thick oocyst wall(TW), sporozoites(SP) and residual body(REB). Transverse sections of sporozoites comprising nucleus(N) and small amylopectin granules(AP), ×24,000.

Figs. 9-10. Nomarski interference-contrast photomicrographs of developmental stages of Cryptosporidium baileyi in mucosal scrapings of the bursa of Fabricius of an experimentally infected 2-day-old chicken. MO, mature oocyst; SP, sporozoite, All figures ×1,500.

Tables


	Table 1 Patent period and peak time of oocyst discharge in chicken inoculated with medium type of Cryptosporidium oocysts in Korean chicken origin


	Table 2 Dimensions of medium type of Cryptosporidium oocysts in Korean chicken origin

Abbreviations


CM	cytoplasmic membrane of parasite
D	dense band
EC	epithelial cell
FO	feeder organelle
HMI	mitochondria of host cell
IB	indented border
MA	macrogamete
MV	microvillus
O	oocyst
PV	parasitophorous vacuole
PVM	parasitophorous vacuolar membrane
T	trophozoite
VM	villous membrane
YO	young oocyst

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