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Activities of brush border membrane bound enzymes of the small intestine in Metagonimus yokogawai infection in mice
S T Hong,J R Yu,N H Myong,*J Y Chai and S H Lee
Institute of Endemic Diseases and Department of Parasitology, Seoul National University College of Medicine, Seoul 110-460, Korea.
*Department of Anatomic Pathology, Korea Cancer Center Hospital, Seoul 139-240, Korea.
Abstract
The present study intended to evaluate the influences of Metagonimus yokogawai on the activities of brush border membrane bound enzymes of the small intestine. Mice were infected with 500 metacercariae respectively, and the worm recovery, morphological changes and enzyme activities were observed chronologically. A part of them were followed after the treatment. Recovered worms decreased in number continuously after the infection, and they were less than 10% after 2 weeks and almost zero after 28 weeks. Villous atrophy and stromal inflammation were found at two locations of the proximal jejunum from 2 weeks to 4 weeks after the infection. The enzymes, alkaline phosphatase, leucine aminopeptidase and disaccharidases (sucrase, lactase, maltase, and trehalase), showed lowered activities in the duodenum and proximal jejunum of the infected mice but they increased in the distal jejunum for the first two weeks. From three weeks after the infection, the activities were gradually recovered. In one week treated mice, they recovered the activities at 2 weeks from the treatment, but there found no differences of the activities between the 3 week treated group and infected controls. The present data reveal that M. yokogawai infection induces degenerative changes of the host's intestinal mucosa not only morphologically but functionally during the initial phase of infection. The lowered enzyme activities in acute metagonimiasis should be associated with malabsorption and diarrhea.
Figures
Fig. 1 The mean numbers of recovered worms per mouse.
Fig. 2 The enzyme activities in the duodenum of mice.
Fig. 3 The enzyme activities in the proximal jejunum of mice.
Fig. 4 The enzyme activities in the distal jejunum of mice.
Tables
Table 1 The numbers of mice used form the experiment
Table 2-1 Microscopic findings of the jejunal mucosa(JI)* by the duration of infection and treatment
Table 2-2 Microscopic findings of the jejunal mucosa(JII)* by the duration of infection and treatment
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