The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine. Its third fraction was purified by affinity chromatography using a monoclonal antibody as ligand. When observed by SDS-PAGE, the purified protein was composed of 2 bands of 36 kDa and 29 kDa which were found already as the most sensitive components in the crude extract by immunoblots with patients sera. The quality of the purified antigen was evaluated in comparison with the crude extract by enzyme-linked immunosorbent assay (ELISA) for the specific (IgG) antibody in sera of human sparganosis, other parasitic and neurologic diseases, and normal control. When the purified antigen was used, the sensitivity was not altered but remained high (96.4%) while the specificity was increased from 86.8% to 96.9%.
Citations
Citations to this article as recorded by
Human Sparganosis in Korea Jeong-Geun Kim, Chun-Seob Ahn, Woon-Mok Sohn, Yukifumi Nawa, Yoon Kong Journal of Korean Medical Science.2018;[Epub] CrossRef
Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera Moizur Rahman, Eung-Goo Lee, Young-An Bae Parasitology International.2011; 60(2): 139. CrossRef
Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana Jing Cui, Nan Li, Zhong Quan Wang, Peng Jiang, Xi Meng Lin Parasitology Research.2011; 108(6): 1551. CrossRef
A Case of Oular Sparganosis in Korea Jae Wook Yang, Jeong Heon Lee, Mi Seon Kang Korean Journal of Ophthalmology.2007; 21(1): 48. CrossRef
A review of human sparganosis in Thailand Viroj Wiwanitkit International Journal of Infectious Diseases.2005; 9(6): 312. CrossRef
A seroepidemiologic survey for human sparganosis in Gangweon-do Kyu-Jae Lee, Yong-Tae Bae, Dong-Heui Kim, Young-Kun Deung, Yong-Suk Ryang The Korean Journal of Parasitology.2002; 40(4): 177. CrossRef
IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho The Korean Journal of Parasitology.2000; 38(3): 145. CrossRef
Characterization of three neutral proteases ofSpirometra mansoniplerocercoid Y. Kong, Y.-B. Chung, S.-Y. Cho, S.-H. Choi, S.-Y. Kang Parasitology.1994; 108(3): 359. CrossRef
Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis N Morakote, Y Kong The Korean Journal of Parasitology.1993; 31(2): 169. CrossRef
Eosinophilic Cystitis Caused by Vesical Sparganosis: A Case Report Seung J. Oh, Je G. Chi, Sang E. Lee Journal of Urology.1993; 149(3): 581. CrossRef
Immunohistochemical localization of 36 and 29 kDa proteins in sparganum L S Kim, Y Kong, S Y Kang, S Y Cho The Korean Journal of Parasitology.1992; 30(1): 25. CrossRef
Isolation and partial characterization of cysteine proteinase from sparganum C Y Song, D H Choi, T S Kim, S H Lee The Korean Journal of Parasitology.1992; 30(3): 191. CrossRef
Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum C H Kim, W S Choi The Korean Journal of Parasitology.1991; 29(4): 339. CrossRef
Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography Y Kong, S Y Kang, S Y Cho The Korean Journal of Parasitology.1991; 29(1): 1. CrossRef
Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies T S Yong, K Im, P R Chung The Korean Journal of Parasitology.1991; 29(3): 293. CrossRef
