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Experimental activation of cryptosporidiosis in mice by immunosuppression
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Korean J Parasito > Volume 28(1):1990 > Article

Original Article
Korean J Parasitol. 1990 Mar;28(1):31-37. Korean.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.1.31
Copyright © 1990 by The Korean Society for Parasitology
Experimental activation of cryptosporidiosis in mice by immunosuppression
Jong-Yil Chai,Son Moon Shin,*Chong Ku Yun,**Jae-Ran Yu and Soon-Hyung Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-460, Korea.
Abstract

Cryptosporidium, a coccidian parasite first described by Tyzzer (1907) from a laboratory mouse, has become an important human enteric pathogen causing overwhelming diarrhea especially in immunocompromised patients such as AIDS. This parasite has been reported from over 20 countries and is recognized as a cosmopolitan species. In Korea, however, there has been no report on human as well as animal cryptosporidiosis. This study was performed so as to verify the presence of Cryptosporidium in Korea by activating the parasite from laboratory mice by immunosuppression.

Total 65 conventionally-bred ICR mice including a control (5 mice) and 3 experimental groups (20 each) were used for this study. Group I was immunosuppressed with prednisolone injection (1 mg IM, every other day) for 7 weeks. Group II (prednisolone injection and tetracycline administration) and Group III (prednisolone injection and trimethoprim-sulfamethoxazole administration) were prepared to observe the effect of antibacterial agents on the activation of cryptosporidiosis.

In fecal examinations of mice Cryptosporidium oocysts (4-6 µm in size) were detected from 1 week after the start of immunosuppression and the mice began to die. In H-E stained tissue sections of the lower jejunum, numerous very small (2-4 µm), dense, ovoid or spherical, slightly basophilic bodies were seen attached on the free border of mucosal epithelial cells. In scanning and transmission electron microscopic observations, these organisms were identified as various developmental stages of Cryptosporidium. The species is considered to be C. parvum.(ABSTRACT TRUNCATED AT 250 WORDS)

Figures


Figs. 1-2
Fig. 1. A Cryptosporidium oocyst (O) containing 4 sporozoites, modified acid-fast stain.

Fig. 2. Lower jejunal section of a mouse showing numerous Cryptosporidium developmental stages(arrows) on the epithelial surface of villi. H & E stain.



Figs. 3-6
Fig. 3. Scanning electron microscopy of Cryptosporidium (arrows) attached on the epithelial surface of jejunal vilii.

Fig. 4. Scanning electron microscopy of a type I meront with eight merozoites(m).

Fig. 5. Transmission electron micrograph of a developing trophozoite surrounded by a pellicle(p), and with a large nucleus(n) and prominent nucleolus(nu).

Fig. 6. Transmission electron micrograph of an early macrogamete surrounded by a pellicle(p), and with various sized granules.


Tables


Table 1
The results of stool examination for Cryptosporidium oocysts by modified acid-fast stain in 3 experimental groups of mice

References
1. Andreani T, Modigliani R, le Charpentier Y, Galian A, Brouet JC, Liance M, Lachance JR, Messing B, Vernisse B. Acquired immunodeficiency with intestinal cryptosporidiosis: possible transmission by Haitian whole blood. Lancet 1983;1(8335):1187–1191.
  
2. Blumberg RS, Kelsey P, Perrone T, Dickersin R, Laquaglia M, Ferruci J. Cytomegalovirus- and Cryptosporidium-associated acalculous gangrenous cholecystitis. Am J Med 1984;76(6):1118–1123.
  
3. Casemore DP, Armstrong M, Sands RL. Laboratory diagnosis of cryptosporidiosis. J Clin Pathol 1985;38(12):1337–1341.
  
4. Claman HN. How corticosteroids work. J Allergy Clin Immunol 1975;55(3):145–151.
  
5. Current WL, Reese NC, Ernst JV, Bailey WS, Heyman MB, Weinstein WM. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission. N Engl J Med 1983;308(21):1252–1257.
  
6. Dobbins WO 3rd, Weinstein WM. Electron microscopy of the intestine and rectum in acquired immunodeficiency syndrome. Gastroenterology 1985;88(3):738–749.
 
7. Fayer R, Ungar BL. Cryptosporidium spp. and cryptosporidiosis. Microbiol Rev 1986;50(4):458–483.
 
8. Forgacs P, Tarshis A, Ma P, Federman M, Mele L, Silverman ML, Shea JA. Intestinal and bronchial cryptosporidiosis in an immunodeficient homosexual man. Ann Intern Med 1983;99(6):793–794.
 
9. Janoff EN, Reller LB. Cryptosporidium species, a protean protozoan. J Clin Microbiol 1987;25(6):967–975.
 
10. Levine ND. Taxonomy and review of the coccidian genus Cryptosporidium (protozoa, apicomplexa). J Protozool 1984;31(1):94–98.
 
11. Ma P, Soave R. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea. J Infect Dis 1983;147(5):824–828.
  
12. Meisel JL, Perera DR, Meligro C, Rubin CE. Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient. Gastroenterology 1976;70(6):1156–1160.
 
13. Nime FA, Burek JD, Page DL, Holscher MA, Yardley JH. Acute enterocolitis in a human being infected with the protozoan Cryptosporidium. Gastroenterology 1976;70(4):592–598.
 
14. Pohlenz J, Moon HW, Cheville NF, Bemrick WJ. Cryptosporidiosis as a probable factor in neonatal diarrhea of calves. J Am Vet Med Assoc 1978;172(4):452–457.
 
15. Soave R, Danner RL, Honig CL, Ma P, Hart CC, Nash T, Roberts RB. Cryptosporidiosis in homosexual men. Ann Intern Med 1984;100(4):504–511.
 
16. Tyzzer EE. Proc Soc Exp Biol Med 1907;5:12–13.
17. Tyzzer EE. Arch Protistenkd 1912;26:349–412.
18. Tzipori S, Angus KW, Campbell I, Gray EW. Cryptosporidium: evidence for a single-species genus. Infect Immun 1980;30(3):884–886.
 
19. Tzipori S, Angus KW, Gray EW, Campbell I. Vomiting and diarrhea associated with cryptosporidial infection. N Engl J Med 1980;303(14):818.
 
20. Upton SJ, Current WL. The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. J Parasitol 1985;71(5):625–629.
  
21. Wagner ED, et al. Jpn J Parasitol 1986;35(4):273–278.
22. Weisburger WR, Hutcheon DF, Yardley JH, Roche JC, Hillis WD, Charache P. Cryptosporidiosis in an immunosuppressed renal-transplant recipient with IgA deficiency. Am J Clin Pathol 1979;72(3):473–478.
 
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