Abstract
Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and centrifuging 24-week old adult worms at 10,000 rpm for 60 minutes in phosphate buffered saline (PBS, pH 7.2) containing phenyl methyl sulfonyl fluoride (PMSF). Gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed and blotted electrophoretically onto a sheet of nitrocellulose paper. The sheet was cut into strips and exposed to sera diluted 1: 200 with PBS. SDS-PAGE showed 26 protein bands ranging 229 to 10 kDa. Of them 229, 91, 60, 50, 35-31, 27, 25, 21, 17, 11 and 10 kDa components showed positive reaction with serum antibody of patients with P. westermani. Sera of patients infected with Clonorchis sinensis reacted with 35-31, 19, and 11 kDa bands. Human sera from cysticercosis and diphyllobothriasis cases showed non-specific cross reactions with 229, 35-31, 27, 25 and 17 kDa bands. Protein bands of 91, 60, 21 and 10 kDa showed strong positive reaction without cross reactions with sera from other helminthic infections.
Citations
Citations to this article as recorded by
- Infection with Paragonimus westermani of boar-hunting dogs in Western Japan maintained via artificial feeding with wild boar meat by hunters
Takao IRIE, Yohei YAMAGUCHI, Pham Ngoc DOANH, Zhi Hong GUO, Shigehisa HABE, Yoichiro HORII, Nariaki NONAKA
Journal of Veterinary Medical Science.2017; 79(8): 1419. CrossRef - Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies
T S Yong, J H Seo, I S Yeo
The Korean Journal of Parasitology.1993; 31(2): 141. CrossRef - Protein composition and antigenicity of the tegument from Paragonimus westermani
S I Kim, S Y Cho
The Korean Journal of Parasitology.1993; 31(3): 269. CrossRef - Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani
Y Kong, C Y Park, S Y Kang, S Y Cho
The Korean Journal of Parasitology.1992; 30(2): 91. CrossRef - Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth
H J Rim, S J Kim, M G Yang
The Korean Journal of Parasitology.1992; 30(4): 309. CrossRef - Molecular weight of major component proteins in crude saline extract of adult Paragonimus westermani
Y Kong, WB Kim, SY Kang, SY Cho
The Korean Journal of Parasitology.1991; 29(2): 113. CrossRef - Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies
S Y Kang, Y Kong, S Y Cho
The Korean Journal of Parasitology.1991; 29(4): 363. CrossRef - Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies
T S Yong, K Im, P R Chung
The Korean Journal of Parasitology.1991; 29(3): 293. CrossRef - Antigenic localities in the tissues of Metagonimus yokogawai observed by immunogoldlabeling method
H Ahn, H J Rim, S J Kim
The Korean Journal of Parasitology.1991; 29(3): 245. CrossRef - Antigenic localities in the tissues of the young adult worm of Paragonimus westermani using immunogold labeling method
O S Kwon, J S Lee, H J Rim, S J Kim
The Korean Journal of Parasitology.1991; 29(1): 31. CrossRef - Paragonimus and paragonimiasis in Korea
D W Choi
The Korean Journal of Parasitology.1990; 28(Suppl): 79. CrossRef - Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique
K H Joo, S C Hong, M S Chung, H J Rim
The Korean Journal of Parasitology.1989; 27(1): 1. CrossRef - Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani
S H Lee, S H Sung, J Y Chai
The Korean Journal of Parasitology.1989; 27(2): 109. CrossRef
