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Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis
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Korean J Parasito > Volume 26(1):1988 > Article

Original Article
Korean J Parasitol. 1988 Mar;26(1):27-32. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.1.27
Copyright © 1988 by The Korean Society for Parasitology
Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis
Kee Seon Eom,Seung-Yull Cho,* and Han Jong Rim**
Department of Parasitology, College of Medicine, Chungbuk National University, Cheongju 360-763, Korea.
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Department of Parasitology, College of Medicine, Korea University, Seoul 110-522, Korea.
Abstract

The applicability of indirect immunofluorescent antibody test (IFAT) was compared with enzyme-linked immunosorbent assay (ELISA) in sera from 163 cases of confirmed neurocysticercosis, 101 other neurologic and parasitic diseases and 100 normal controls. As antigen, frozen sections of a Taenia solium metacestode from a human brain was used in IFAT and cystic fluid was used in ELISA. For the detection of specific IgG antibody, IFAT was equally sensitive (89. 6%) and specific (85. l%) as ELISA. The antibody titers by IFAT were correspondingly increased with mean absorbance of ELISA. The corresponding rate of positivity in the two techniques was 90.8%. Except for the difficulty in detecting antibodies in cerebrospinal fluid (CSF), IFAT was concluded to be very useful for the serodiagnosis of human neurocysticercosis.

Figures


Fig. 1
Relations between absorbance by ELISA and IFAT titers in detecting specific IgG antibody in sera of 163 confirmed neurocysticercosis cases. Open circle (◦): serum (-)/CSF (+) cases by ELISA

Tables


Table 1
Results of serum IFAT in 163 confirmed neurocysticercosis patients and 100normal control cases


Table 2
Comparative incidence of cross reactions by ELISA and IFAT for specific IgG antibodies in sera of other neurologic and parasitic diseases

References
1. Baily GG, Mason PR, Trijssenar FE, Lyons NF. Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg 1988;82(2):295–299.
  
2. Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.
 
3. Choi BK, et al. Chung-Ang J Med 1986;11(2):135–146.
6. González-Barranco D, Sandoval-Islas ME, Trujillo-Valdes VM. Indirect immunofluorescense reaction in cysticercosis. Arch Invest Med (Mex) 1978;9(1):51–58.
8. Kang OJ, et al. Korea Univ Med J 1987;24(1):85–97.
9. Konovalova LM. [Search for and use of new methods for immunodiagnosis of human cysticercosis]. Med Parazitol (Mosk) 1973;42(5):536–542.
 
10. Rydzewski AK, Chisholm ES, Kagan IG. Comparison of serologic tests for human cysticercosis by indirect hemagglutination, indirect immunofluorescent antibody, and agar gel precipitin tests. J Parasitol 1975;61(1):154–155.
  
11. Wilson M, Sulzer AJ, Walls KW. Modified antigens in the indirect immunofluorescence test for schistosomiasis. Am J Trop Med Hyg 1974;23(6):1072–1076.
 
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