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Hak-Sun Yu	8 Articles
Negligible Egg Positive Rate of Enterobius vermicularis and No Detection of Head Lice among Orphanage Children in Busan and Ulsan, Korea (2014) Dong-Hee Kim, Hyun-Mi Son, Sang Hwa Lee, Mi Kyung Park, Shin Ae Kang, Sang Kyun Park, Jun-Ho Choi, Jung Ha Park, Hak-Sun Yu Korean J Parasitol 2015;53(4):497-499. Published online August 25, 2015 DOI: https://doi.org/10.3347/kjp.2015.53.4.497 Abstract PDF To determine whether pinworm infections and head lice infestations spread among children in orphanages, 117 children from 4 orphanages in Busan-si and Ulsan-si, Korea, were examined for enterobiasis and head lice infestation between January and February 2014. The overall rate of Enterobius vermicularis egg positivity was 0.85%, whereas none of the children had head lice infestations. The rate of pinworm infection was much lower among the orphanage children compared to the rates observed in previous studies among kindergarten and primary school students. Moreover, the risk factors for enterobiasis were less frequent among these subjects than previously reported. The personal hygiene and health of the orphanage children were supervised by a regular, employed nurse through a health education program. In conclusion, pinworm infection was efficiently controlled among the children in orphanages, and this might be related to good personal hygiene practices in Korea. Citations Citations to this article as recorded by Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai The Korean Journal of Parasitology.2021; 59(4): 421. CrossRef A systematic scoping review of hygiene behaviors and environmental health conditions in institutional care settings for orphaned and abandoned children Michelle Moffa, Ryan Cronk, Donald Fejfar, Sarah Dancausse, Leslie Acosta Padilla, Jamie Bartram Science of The Total Environment.2019; 658: 1161. CrossRef Status of common parasitic diseases in Korea in 2019 Sun Huh Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef Prevalence ofEnterobius vermicularisamong preschool children in 2003 and 2013 in Xinxiang city, Henan province, Central China Shuai Wang, Zhijun Yao, Yichen Hou, Dong Wang, Haizhu Zhang, Jingbo Ma, Luwen Zhang, Shiguo Liu Parasite.2016; 23: 30. CrossRef 9,260 View 132 Download 5 Web of Science Crossref Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani Joon-Hyuck Choi, Jae-Hyuk Lee, Hak-Sun Yu, Hae-Jin Jeong, Jin Kim, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2006;44(3):187-196. Published online September 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.3.187 Abstract PDF The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and reproduction of P. westermani. In this study, a cDNA clone encoding a 47 kDa hemoglobinase of P. westermani was characterized by sequencing analysis, and its localization was investigated immunohistochemically. The phylogenetic tree prepared based on the hemoglobinase gene showed high homology with hemoglobinases of Fasciola hepatica and Schistosoma spp. Moreover, recombinant P. westermani hemoglobinase degradaded human hemoglobin at acidic pH (from 3.0 to 5.5) and its activity was almost completely inhibited by E-64, a cysteine proteinase inhibitor. Immunohistochemical studies showed that P. westermani hemoglobinase was localized in the epithelium of the adult worm intestine implying that the protein has a specific function. These observations suggest that hemoglobinase may act as a digestive enzyme for acquisition of nutrients from host hemoglobin. Further investigations may provide insights into hemoglobin catabolism in P. westermani. Citations Citations to this article as recorded by Multi-stage transcriptome profiling of the neglected food-borne echinostome Artyfechinostomum sufrartyfex reveal potential diagnostic and drug targets Suman Dahal, Pratibha Gour, Saurabh Raghuvanshi, Yugal Kishore Prasad, Dipshikha Saikia, Sudeep Ghatani Acta Tropica.2022; 233: 106564. CrossRef Characterization and localization of antigens for serodiagnosis of human paragonimiasis Kurt C. Curtis, Kerstin Fischer, Young-Jun Choi, Makedonka Mitreva, Gary J. Weil, Peter U. Fischer Parasitology Research.2021; 120(2): 535. CrossRef Cysteine proteases as digestive enzymes in parasitic helminths Conor R. Caffrey, Louise Goupil, Karina M. Rebello, John P. Dalton, David Smith, Aaron R. Jex PLOS Neglected Tropical Diseases.2018; 12(8): e0005840. CrossRef Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl Experimental Parasitology.2013; 135(2): 297. CrossRef Paragonimus worm from a New Guinea native in 1926 Wenlin Wang, David Blair, Tian Min, Fang Li, Dianhua Wang Asian Pacific Journal of Tropical Medicine.2011; 4(1): 76. CrossRef Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis Thewarach Laha, Jittiyawadee Sripa, Banchob Sripa, Mark Pearson, Leon Tribolet, Sasithorn Kaewkes, Paiboon Sithithaworn, Paul J. Brindley, Alex Loukas International Journal of Infectious Diseases.2008; 12(6): e49. CrossRef 10,210 View 79 Download Crossref Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2005;43(1):7-13. Published online March 20, 2005 DOI: https://doi.org/10.3347/kjp.2005.43.1.7 Abstract PDF The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes. Citations Citations to this article as recorded by The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections Paul A. Fuerst Microorganisms.2023; 11(2): 295. CrossRef On the diversity and clinical importance of Acanthamoeba spp. from Group 1 Daniele Corsaro Parasitology Research.2021; 120(6): 2057. CrossRef Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea) Stas Malavin, Lyubov Shmakova European Journal of Protistology.2020; 73: 125671. CrossRef Update on Acanthamoeba phylogeny Daniele Corsaro Parasitology Research.2020; 119(10): 3327. CrossRef Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed Acta Tropica.2011; 117(1): 23. CrossRef Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a non-contact-lens wearer in Turkey Hatice Ertabaklar, Meral Türk, Volkan Dayanir, Sema Ertuğ, Julia Walochnik Parasitology Research.2007; 100(2): 241. CrossRef 9,210 View 78 Download Crossref Purification and characterization of a 33 kDa serine protease from Acanthamoeba lugdunensis KA/E2 isolated from a Korean keratitis patient Hyo-Kyung Kim, Young-Ran Ha, Hak-Sun Yu, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2003;41(4):189-196. Published online December 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.4.189 Abstract PDF In order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient. The ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on CM-Sepharose, Sephacryl S-200, and mono Q-anion exchange column. The purified 33 kDa protease had a pH optimum of 8.5 and a temperature optimum of 55℃. Phenylmethylsulfonylfluoride and 4-(2-Aminoethyl)-benzenesulfonyl-fluoride, both serine protease specific inhibitors, inhibited almost completely the activity of the 33 kDa protease whereas other classes of inhibitors did not affect its activity. The 33 kDa enzyme degraded various extracellular matrix proteins and serum proteins. Our results strongly suggest that the 33 kDa serine protease secreted from this keratopathogenic Acanthamoeba play important roles in the pathogenesis of amoebic keratitis, such as in corneal tissue invasion, immune evasion and nutrient uptake. Citations Citations to this article as recorded by Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver Processes.2023; 11(9): 2620. CrossRef The gene expression and proteomic profiling of Acanthamoeba isolates Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta Experimental Parasitology.2023; 255: 108630. CrossRef Comparative Surfaceome Analysis of Clonal Histomonas meleagridis Strains with Different Pathogenicity Reveals Strain-Dependent Profiles Marcelo de Jesus Ramires, Karin Hummel, Tamas Hatfaludi, Petra Riedl, Michael Hess, Ivana Bilic Microorganisms.2022; 10(10): 1884. CrossRef Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins Abigail Betanzos, Cecilia Bañuelos, Esther Orozco Genes.2019; 10(8): 618. CrossRef Acanthamoeba spp. un agente oportunista en infecciones humanas Martín Cabello-Vílchez Revista de Investigación de la Universidad Privada Norbert Wiener.2019; 4(1): 11. CrossRef Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells Showgy Y. Ma’ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G. Svärd, Armando Jardim PLOS Neglected Tropical Diseases.2017; 11(12): e0006120. CrossRef Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut Goutam Banerjee, Sandip Mukherjee, Shelley Bhattacharya, Arun K. Ray Arabian Journal for Science and Engineering.2016; 41(1): 9. CrossRef Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition Gaurav Kumar Pal, Suresh PV RSC Advances.2016; 6(40): 33763. CrossRef Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases Yu-Zhong Zhang, Li-Yuan Ran, Chun-Yang Li, Xiu-Lan Chen, F. E. Löffler Applied and Environmental Microbiology.2015; 81(18): 6098. CrossRef Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong Experimental Parasitology.2014; 143: 69. CrossRef Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism Li-Yuan Ran, Hai-Nan Su, Ming-Yang Zhou, Lei Wang, Xiu-Lan Chen, Bin-Bin Xie, Xiao-Yan Song, Mei Shi, Qi-Long Qin, Xiuhua Pang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang Journal of Biological Chemistry.2014; 289(9): 6041. CrossRef Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family Li‐Yuan Ran, Hai‐Nan Su, Guo‐Yan Zhao, Xiang Gao, Ming‐Yang Zhou, Peng Wang, Hui‐Lin Zhao, Bin‐Bin Xie, Xi‐Ying Zhang, Xiu‐Lan Chen, Bai‐Cheng Zhou, Yu‐Zhong Zhang Molecular Microbiology.2013; 90(5): 997. CrossRef Proteases fromEntamoebaspp. and Pathogenic Free-Living Amoebae as Virulence Factors Jesús Serrano-Luna, Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortiz-Estrada, Mireya de la Garza Journal of Tropical Medicine.2013; 2013: 1. CrossRef Host-Parasite Interaction: Parasite-Derived and -Induced Proteases That Degrade Human Extracellular Matrix Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortíz-Estrada, Mireya de la Garza, Jesús Serrano-Luna Journal of Parasitology Research.2012; 2012: 1. CrossRef Distribution of Proteolytic Activity in the Different Protein Fractions of Tropical Shrimp Head Waste Raghu Ganugula, Rupsankar Chakrabarti, Krothapalli Raja Surya Sambasiva Rao Food Biotechnology.2008; 22(1): 18. CrossRef Protease activity in extracellular products secreted in vitro by trophozoites of Giardia duodenalis Thaís Batista de Carvalho, Érica Boarato David, Silvana Torossian Coradi, Semíramis Guimarães Parasitology Research.2008; 104(1): 185. CrossRef Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence Won-Tae Kim, Hyun-Hee Kong, Young-Ran Ha, Yeon-Chul Hong, Hae Jin Jeong, Hak Sun Yu, Dong-Il Chung The Korean Journal of Parasitology.2006; 44(4): 321. CrossRef Detection of a serine proteinase gene in Acanthamoeba genotype T6 (Amoebozoa: Lobosea) Marion Blaschitz, Martina Köhsler, Horst Aspöck, Julia Walochnik Experimental Parasitology.2006; 114(1): 26. CrossRef Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong Eukaryotic Cell.2006; 5(1): 125. CrossRef Étude de l’effet des oligomères procyanidoliques sur la fibrillogénèse de la cornée A.M. Robert, L. Robert, G. Renard Journal Français d'Ophtalmologie.2005; 28(10): 1017. CrossRef Expression of the nfa1 Gene Cloned from Pathogenic Naegleria fowleri in Nonpathogenic N. gruberi Enhances Cytotoxicity against CHO Target Cells In Vitro Seok-Ryoul Jeong, Sang-Chul Lee, Kyoung-Ju Song, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-il Im, Ho-Joon Shin Infection and Immunity.2005; 73(7): 4098. CrossRef Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood–brain barrier Selwa Alsam, James Sissons, Samantha Jayasekera, Naveed Ahmed Khan Journal of Infection.2005; 51(2): 150. CrossRef 8,897 View 87 Download Crossref Cloning and characterization of Giardia intestinalis cyclophilin Hak-Sun Yu, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2002;40(3):131-138. Published online September 30, 2002 DOI: https://doi.org/10.3347/kjp.2002.40.3.131 Abstract PDF The cyclophilins (Cyps) are family members of proteins that exhibit peptidyl-prolyl cis-trans isomerase (PPIase, EC 5.2.1.8) activity and bind the immunosuppressive agent cyclosprin A (CsA) in varying degrees. During the process of random sequencing of a cDNA library made from Giardia intestinalis WB strain, the cyclophilin gene (gicyp1) was isolated. An open reading frame of gicyp1 gene was 576 nucleotides, which corresponded to a translation product of 176 amino acids (Gicyp1). The identity with other Cyps was about 58-71%. The 13 residues that constituted the CsA binding site of human cyclophilin were also detected in the amino acid sequence of Gicyp1, including tryptophan residue essential for the drug binding. The single copy of the gicyp1 gene was detected in the G. intestinalis chromosome by southern hybridization analysis. Recombinant Gicyp1 protein clearly accelerated the rate of cis → trans isomerization of the peptide substrate and the catalysis was completely inhibited by the addition of 0.5 ?M CsA. Citations Citations to this article as recorded by Cyclophilins as key players in protozoan parasite infections Reza Mansouri, Enrique Granado-Aparicio, Claudia Alcedo, Julio López-Abán, Reza Shafiei, Antonio Muro, Raúl Manzano-Román, Sajad Rashidi Parasites & Vectors.2025;[Epub] CrossRef Insights into Peptidyl-Prolyl cis-trans Isomerases from Clinically Important Protozoans: From Structure to Potential Biotechnological Applications Verónica Aranda-Chan, Rosa Elena Cárdenas-Guerra, Alejandro Otero-Pedraza, Esdras Enoc Pacindo-Cabrales, Claudia Ivonne Flores-Pucheta, Octavio Montes-Flores, Rossana Arroyo, Jaime Ortega-López Pathogens.2024; 13(8): 644. CrossRef Molecular aspects of cyclophilins mediating therapeutic actions of their ligands Andrzej Galat, Jacqueline Bua Cellular and Molecular Life Sciences.2010; 67(20): 3467. CrossRef Peptidyl-prolyl cis–trans isomerases (immunophilins) and their roles in parasite biochemistry, host–parasite interaction and antiparasitic drug action Angus Bell, Paul Monaghan, Antony P. Page International Journal for Parasitology.2006; 36(3): 261. CrossRef A Cyclophilin from Griffithsia japonica Has Thermoprotective Activity and Is Affected by CsA Eun Kyung Cho, Yoo Kyung Lee, Choo Bong Hong Molecules and Cells.2005; 20(1): 142. CrossRef 8,619 View 81 Download Crossref Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi Yeon-Chul Hong, Mi-Yul Hwang, Ho-Cheol Yun, Hak-Sun Yu, Hyun-Hee Kong, Tai-Soon Yong, Dong-Il Chung Korean J Parasitol 2002;40(1):17-24. Published online March 31, 2002 DOI: https://doi.org/10.3347/kjp.2002.40.1.17 Abstract PDF We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an A. healyi cDNA library screening was amplified by PCR using degenerate oligonucleotide primers designed on the basis of conserved amino acids franking the active sites of cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteinases. Cysteine proteinase gene of A. healyi (AhCP1) was composed of 330 amino acids with signal sequence, a proposed pro-domain and a predicted active site made up of the catalytic residues, Cys²⁵, His¹⁵⁹, and Asn¹⁷⁵. Deduced amino acid sequence analysis indicates that AhCP1 belong to ERFNIN subfamily of C1 peptidases. By Northern blot analysis, no direct correlation was observed between AhCP1 mRNA expression and virulence of Acanthamoeba, but the gene was expressed at higher level in amoebae isolated from soil than amoeba from clinical samples. These findings raise the possibility that Ahcp1 protein may play a role in protein metabolism and digestion of phagocytosed bacteria or host tissue debris rather than in invasion of amoebae into host tissue. Citations Citations to this article as recorded by Amoebic encephalitis within Australia Samantha C. Carija, Avram Levy, Graham Weaire‐Buchanan, Terence Lee, Robin Woodward, Jake Gazeley, Troy A. Edwards, Jason M. Dyke, Vicki Fabian, Katherine Norton, Andrew Chapman, Paul R. Ingram Internal Medicine Journal.2025; 55(1): 143. CrossRef Biological characteristics and pathogenicity of Acanthamoeba Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li Frontiers in Microbiology.2023;[Epub] CrossRef Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3 Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng Parasites & Vectors.2020;[Epub] CrossRef Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan Acta Tropica.2020; 211: 105618. CrossRef Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins Abigail Betanzos, Cecilia Bañuelos, Esther Orozco Genes.2019; 10(8): 618. CrossRef Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, Hương Giang Lê, Thị Lam Thái, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na The Korean Journal of Parasitology.2018; 56(5): 409. CrossRef Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral Infection and Immunity.2010; 78(9): 4001. CrossRef Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong Eukaryotic Cell.2008; 7(9): 1513. CrossRef Differentially expressed genes of Acanthamoeba castellanii during encystation Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef Acanthamoeba: biology and increasing importance in human health Naveed Ahmed Khan FEMS Microbiology Reviews.2006; 30(4): 564. CrossRef Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong Eukaryotic Cell.2006; 5(1): 125. CrossRef Pathogenic free-living amoebae in Korea Ho-Joon Shin, Kyung-il Im The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef 8,579 View 96 Download Crossref Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea Hak-Sun Yu, Kyung-Hee Choi, Hyo-Kyung Kim, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 2001;39(2):161-170. Published online June 30, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.2.161 Abstract PDF We conducted both the small subunit ribosomal DNA (SSU rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and mitochondrial (mt) DNA RFLP analyses for a genetic characterization of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea. Twenty-three strains of Acanthamoeba from the American Type Culture Collection and twelve clinical isolates from Korean patients were used as reference strains. Thirty-nine isolates from contact lens storage cases were classified into seven types (KA/LS1, KA/LS2, KA/LS4, KA/LS5, KA/LS7, KA/LS18, KA/LS31). Four types (KA/LS1, KA/LS2, KA/LS5, KA/LS18) including 33 isolates were regarded as A. castellanii complex by riboprints. KA/LS1 type was the most predominant (51.3%) in the present survey area, followed by KA/LS2 (20.9%), and KA/LS5 (7.7%) types. Amoebae of KA/LS1 type had the same mtDNA RFLP and riboprint patterns as KA/E2 and KA/E12 strains, clinical isolates from Korean keratitis patients. Amoebae of KA/LS2 type had the identical mtDNA RFLP patterns with A. castellanii Ma strain, a corneal isolate from an American patient as amoebae of KA/LS5 type, with KA/E3 and KA/E8 strains from other Korean keratitis patients. Amoebae of KA/LS18 type had identical patterns with JAC/E1, an ocular isolate from a Japanese patient. Three types, which remain unidentified at species level, were not corresponded with any clinical isolate in their mtDNA RFLP and riboprint patterns. Out of 39 isolates analyzed in this study, mtDNA RFLP and riboprint patterns of 33 isolates (84.6%) were identical to already known clinical isolates, and therefore, they may be regarded as potentially keratopathogenic. These results suggest that contact lens wearers in Seoul should pay more attention to hygienic maintenance of contact lens storage cases for the prevention of Acanthamoeba keratitis. Citations Citations to this article as recorded by Parasitological and microbiological assessment of contact lens storage cases: a survey of asymptomatic lens student wearers from five medical specialties in Tunisia, North Africa Sameh Belgacem, Raja Chaâbane-Banaoues, Amira Mejri, Sawsen Ben Ifa, Maha Mastouri, Hamouda Babba BMC Infectious Diseases.2025;[Epub] CrossRef The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis Min Seung Kang, Sangyoon Kim, Da Som Kim, Hak Sun Yu, Ji Eun Lee, Yi Cao PLOS ONE.2023; 18(2): e0281141. CrossRef The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef Adhesion ofAcanthamoebaon Cosmetic Contact Lenses Seung-Mok Lee, Ji-Eun Lee, Da-In Lee, Hak-Sun Yu Journal of Korean Medical Science.2018;[Epub] CrossRef Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Angela Magnet, Thiago Santos Gomes, Carmen Pardinas, Natalia Garcia de Blas, Cruz Sadaba, Eugenia Carrillo, Fernando Izquierdo, José Manuel Benítez del Castillo, Carolina Hurtado, Carmen del Aguila, Soledad Fenoy Parasites & Vectors.2018;[Epub] CrossRef Effect of Multipurpose Solution Combined With Autophagy Inhibitors on Adhesion of Acanthamoeba trophozoites to Silicone Hydrogel Contact Lenses Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee Cornea.2017; 36(12): 1538. CrossRef Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses Ga‐Hyun Lee, Hak‐Sun Yu, Ji‐Eun Lee Ophthalmic and Physiological Optics.2016; 36(2): 93. CrossRef Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses Ga-Hyun Lee, Ji-Eun Lee, Mi-Kyung Park, Hak-Sun Yu Cornea.2016; 35(5): 663. CrossRef Acanthamoeba spp. in Contact Lenses from Healthy Individuals from Madrid, Spain Thiago dos Santos Gomes, Angela Magnet, Fernando Izquierdo, Lucianna Vaccaro, Fernando Redondo, Sara Bueno, Maria Luisa Sánchez, Santiago Angulo, Soledad Fenoy, Carolina Hurtado, Carmen del Aguila, Graham R. Wallace PLOS ONE.2016; 11(4): e0154246. CrossRef 18S ribosomal DNA genotypes of Acanthamoeba species isolated from contact lens cases in the Philippines Windell L. Rivera, Davin Edric V. Adao Parasitology Research.2009; 105(4): 1119. CrossRef Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef Molecular Phylogeny of Acanthamoeba Hyun Hee Kong The Korean Journal of Parasitology.2009; 47(Suppl): S21. 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CrossRef 9,737 View 53 Download Crossref Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene Hak-Sun Yu, Mee-Yul Hwang, Tae-Ook Kim, Ho-Cheol Yun, Tae-Ho Kim, Hyun-Hee Kong, Dong-Il Chung Korean J Parasitol 1999;37(3):181-188. Published online September 30, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.3.181 Abstract PDF We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close interrelationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18S rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18S riboprinting. Acanthamoeba griffini of morphological group 2 branched between pathogenic (A. culbertsoni A-1 and A. healyi OC-3A) and nonpathogenic (A. palestinensis Reich, A. pustulosa GE-3a, A. royreba Oak Ridge, and A lenticulata PD2S) strains of morphological group 3. Among the remaining isolates of morphological group 2, the Chang strain had the identical mitochondrial riboprints as the type strain of A. hatchetti. AA2 and AA1, the type strains of A. divionensis and A. paradivionensis, respectively, had the identical riboprints as A. quina Vil3 and A. castellanii Ma. Although the branching orders of A. castellanii Neff, A. polyphaga P23, A. triangularis SH621, and A. lugdunensis L3a were different from those in 18S riboprinting analysis, the results obtained from this study generally coincided well with those from 18S riboprinting. 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CrossRef Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge Jill M. Schroeder, Gregory C. Booton, John Hay, Ingrid A. Niszl, David V. Seal, Miles B. Markus, Paul A. Fuerst, Thomas J. Byers Journal of Clinical Microbiology.2001; 39(5): 1903. CrossRef Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp Julia Walochnik, Andreas Obwaller, Horst Aspöck Applied and Environmental Microbiology.2000; 66(10): 4408. CrossRef 9,702 View 77 Download Crossref