Parasites, Hosts and Diseases

Skip to main navigation Skip to main content

OPEN ACCESS

Page Path

HOME
BROWSE ARTICLES

Author index detail contents
Hua Zeng	3 Articles
Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae) Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu Korean J Parasitol 2015;53(6):789-793. Published online December 31, 2015 DOI: https://doi.org/10.3347/kjp.2015.53.6.789 Abstract PDF In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum. Citations Citations to this article as recorded by Characterization of tick salivary gland and saliva alphagalactome reveals candidate alpha-gal syndrome disease biomarkers Margarita Villar, Iván Pacheco, Lourdes Mateos-Hernández, Alejandro Cabezas-Cruz, Ala E. Tabor, Manuel Rodríguez-Valle, Albert Mulenga, Katherine M. Kocan, Edmour F. Blouin, José de La Fuente Expert Review of Proteomics.2021; 18(12): 1099. CrossRef Extracellular vesicles induce protective immunity against Trichuris muris R. K. Shears, A. J. Bancroft, G. W. Hughes, R. K. Grencis, D. J. Thornton Parasite Immunology.2018;[Epub] CrossRef New approaches and omics tools for mining of vaccine candidates against vector-borne diseases Josipa Kuleš, Anita Horvatić, Nicolas Guillemin, Asier Galan, Vladimir Mrljak, Mangesh Bhide Molecular BioSystems.2016; 12(9): 2680. CrossRef 9,640 View 90 Download 5 Web of Science Crossref Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum Yonghong Hu, Hua Zeng, Jincheng Zhang, Duo Wang, Dongming Li, Tiantian Zhang, Shujie Yang, Jingze Liu Korean J Parasitol 2014;52(1):93-97. Published online February 19, 2014 DOI: https://doi.org/10.3347/kjp.2014.52.1.93 Abstract PDF Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date, no protective antigens have been characterized nor tested as candidate vaccines against Dermacentor silvarum bites and transmission of associated pathogens. In this study, we cloned the open reading frame (ORF) of D. silvarum 4D8 cDNA (Ds4D8), which consisted of 498 bp encoding 165 amino acid residues. The results of sequence alignments and phylogenetic analysis demonstrated that D. silvarum 4D8 (Ds4D8) is highly conserved showing more than 81% identity of amino acid sequences with those of other hard ticks. Additionally, Ds4D8 containing restriction sites was ligated into the pET-32(a+) expression vector and the recombinant plasmid was transformed into Escherichia coli rosetta. The recombinant Ds4D8 (rDs4D8) was induced by isopropyl β-D-thiogalactopyranoside (IPTG) and purified using Ni affinity chromatography. The SDS-PAGE results showed that the molecular weight of rDs4D8 was 40 kDa, which was consistent with the expected molecular mass considering 22 kDa histidine-tagged thioredoxin (TRX) protein from the expression vector. Western blot results showed that rabbit anti-D. silvarum serum recognized the expressed rDs4D8, suggesting an immune response against rDs4D8. These results provided the basis for developing a candidate vaccine against D. silvarum ticks and transmission of associated pathogens. Citations Citations to this article as recorded by Subolesin gene structure and mRNA isoform diversity in South African R. microplus ticks: Relevance for understanding subolesin-based tick vaccines Elsje Christine Rabie, Christine Maritz-Olivier Ticks and Tick-borne Diseases.2025; 16(4): 102502. CrossRef Transmission-Blocking Vaccines: Focus on Anti-Vector Vaccines against Tick-Borne Diseases Girish Neelakanta, Hameeda Sultana Archivum Immunologiae et Therapiae Experimentalis.2015; 63(3): 169. CrossRef Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae) Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef 9,631 View 95 Download 3 Web of Science Crossref Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis Yonghong Hu, Jincheng Zhang, Shujie Yang, Hui Wang, Hua Zeng, Tiantian Zhang, Jingze Liu Korean J Parasitol 2013;51(3):327-334. Published online June 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.3.327 Abstract PDF Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an α-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks. Citations Citations to this article as recorded by The current strategies and underlying mechanisms in the control of the vector tick, Haemaphysalis longicornis: Implications for future integrated management Chuks F. Nwanade, Min Wang, Sisi Li, Zhijun Yu, Jingze Liu Ticks and Tick-borne Diseases.2022; 13(2): 101905. CrossRef Evaluation on two types of paramyosin vaccines for the control of Haemaphysalis longicornis infestations in rabbits Pin-Xing Wu, Xue-Jiao Cui, Mi-Xue Cao, Li-Hong Lv, Hong-Meng Dong, Shu-Wen Xiao, Jing-Ze Liu, Yong-Hong Hu Parasites & Vectors.2021;[Epub] CrossRef Morphology and ultrastructure changes of Haemaphysalis longicornis Neumann (Acari: Ixodidae) female adult ticks at different developmental stages Fang Wang, Duo Wang, Chao-Zhong Jiang, Na Liang, Yong-Hong Hu, Jing-Ze Liu International Journal of Acarology.2017; 43(4): 308. CrossRef Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits Tian-Tian Zhang, Jin-Cheng Zhang, Xue-Jiao Cui, Jing-Jing Zheng, Ru Li, Fang Wang, Jing-Ze Liu, Yong-Hong Hu Parasites & Vectors.2017;[Epub] CrossRef Paramyosin of canine Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease Bronwyn Campbell, Helder Cortes, Giada Annoscia, Alessio Giannelli, Antonio Parisi, Maria Stefania Latrofa, Filipe Dantas-Torres, Luís Cardoso, Domenico Otranto Parasites & Vectors.2016;[Epub] CrossRef Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae) Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef 10,979 View 69 Download Crossref