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Hyun-Ouk Song	12 Articles
Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis Hyun-Ouk Song Korean J Parasitol 2016;54(1):71-74. Published online February 26, 2016 DOI: https://doi.org/10.3347/kjp.2016.54.1.71 Abstract PDF Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis. Citations Citations to this article as recorded by Trichomonas vaginalis acid sphingomyelinases' theoretical structural analysis shows substrate binding diversity related to protein flexibility and mobility Ana Laura Medina-Nieto, Sairy Yarely Andrade-Guillen, Fátima Berenice Ramírez-Montiel, Fátima Tornero-Gutiérrez, José A. Martínez-Álvarez, Ángeles Rangel-Serrano, Itzel Páramo-Pérez, Naurú Idalia Vargas-Maya, Javier de la Mora, Claudia Leticia Mendoza-Mac Computational Biology and Chemistry.2026; 120: 108601. CrossRef Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites Namrata Anand Frontiers in Parasitology.2024;[Epub] CrossRef Genetic Variability of the Internal Transcribed Spacer and Pyruvate:Ferredoxin Oxidoreductase Partial Gene of Trichomonas vaginalis from Female Patients Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocaña, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos Microorganisms.2023; 11(9): 2240. CrossRef Pyruvate: Ferredoxin oxidoreductase is involved in IgA-related microbiota dysbiosis and intestinal inflammation Kairuo Wang, Yixuan Guo, Yuanyuan Liu, Xiao Cui, Xiang Gu, Lixiang Li, Yanqing Li, Ming Li Frontiers in Immunology.2022;[Epub] CrossRef Microbiota in vaginal health and pathogenesis of recurrent vulvovaginal infections: a critical review Namarta Kalia, Jatinder Singh, Manpreet Kaur Annals of Clinical Microbiology and Antimicrobials.2020;[Epub] CrossRef New light on ancient enzymes – in vitro CO2 Fixation by Pyruvate Synthase of Desulfovibrio africanus and Sulfolobus acidocaldarius Andreas Witt, Roberta Pozzi, Stephan Diesch, Oliver Hädicke, Hartmut Grammel The FEBS Journal.2019; 286(22): 4494. CrossRef Humoral and T cell–mediated immune response against trichomoniasis M. Nemati, N. Malla, M. Yadav, H. Khorramdelazad, A. Jafarzadeh Parasite Immunology.2018;[Epub] CrossRef A Cell Surface Aggregation-Promoting Factor from Lactobacillus gasseri Contributes to Inhibition of Trichomonas vaginalis Adhesion to Human Vaginal Ectocervical Cells Niha Phukan, Anna E. S. Brooks, Augusto Simoes-Barbosa, John H. Adams Infection and Immunity.2018;[Epub] CrossRef Can the pyruvate: ferredoxin oxidoreductase (PFOR) gene be used as an additional marker to discriminate among Blastocystis strains or subtypes? Patricia Alarcon-Valdes, Guiehdani Villalobos, Williams Arony Martinez-Flores, Eduardo Lopez-Escamilla, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Fernando Martinez-Hernandez, Jonnathan Guadalupe Santillan-Benitez, Pablo Maravilla Parasites & Vectors.2018;[Epub] CrossRef Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins Dong Liu, Zhengjiao Yang, Yong Chen, Wei Zhuang, Huanqing Niu, Jinglan Wu, Hanjie Ying Biotechnology for Biofuels.2018;[Epub] CrossRef 9,622 View 93 Download 10 Web of Science Crossref Parasitic Infections Based on 320 Clinical Samples Submitted to Hanyang University, Korea (2004-2011) Sung-Chul Choi, Soo-Young Lee, Hyun-Ouk Song, Jae-Sook Ryu, Myoung-Hee Ahn Korean J Parasitol 2014;52(2):215-220. Published online April 18, 2014 DOI: https://doi.org/10.3347/kjp.2014.52.2.215 Abstract PDF We analyzed 320 clinical samples of parasitic infections submitted to the Department of Environmental Biology and Medical Parasitology, Hanyang University from January 2004 to June 2011. They consisted of 211 nematode infections, 64 trematode or cestode infections, 32 protozoan infections, and 13 infections with arthropods. The nematode infections included 67 cases of trichuriasis, 62 of anisakiasis (Anisakis sp. and Pseudoterranova decipiens), 40 of enterobiasis, and 24 of ascariasis, as well as other infections including strongyloidiasis, thelaziasis, loiasis, and hookworm infecions. Among the cestode or trematode infections, we observed 27 cases of diphyllobothriasis, 14 of sparganosis, 9 of clonorchiasis, and 5 of paragonimiasis together with a few cases of taeniasis saginata, cysticercosis cellulosae, hymenolepiasis, and echinostomiasis. The protozoan infections included 14 cases of malaria, 4 of cryptosporidiosis, and 3 of trichomoniasis, in addition to infections with Entamoeba histolytica, Entamoeba dispar, Entamoeba coli, Endolimax nana, Giardia lamblia, and Toxoplasma gondii. Among the arthropods, we detected 6 cases of Ixodes sp., 5 of Phthirus pubis, 1 of Sarcoptes scabiei, and 1 of fly larva. The results revealed that trichuriasis, anisakiasis, enterobiasis, and diphyllobothriasis were the most frequently found parasitosis among the clinical samples. Citations Citations to this article as recorded by Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn Parasitology Research.2024;[Epub] CrossRef Global prevalence of 4 neglected foodborne trematodes targeted for control by WHO: A scoping review to highlight the gaps Rachel Tidman, Kaushi S. T. Kanankege, Mathieu Bangert, Bernadette Abela-Ridder, Paul R. Torgerson PLOS Neglected Tropical Diseases.2023; 17(3): e0011073. CrossRef Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia Ramon M. Eichenberger, Lian F. Thomas, Sarah Gabriël, Branco Bobić, Brecht Devleesschauwer, Lucy J. Robertson, Anastasios Saratsis, Paul R. Torgerson, Uffe C. Braae, Veronique Dermauw, Pierre Dorny Parasites & Vectors.2020;[Epub] CrossRef Multilocus genotyping of Giardia duodenalis from pigs in Korea Haeseung Lee, ByeongYeal Jung, Jun-Sik Lim, Min-Goo Seo, Seung-Hun Lee, Kwang-Ho Choi, Mi-Hye Hwang, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak Parasitology International.2020; 78: 102154. CrossRef Molecular Identification of Anisakis Larvae Extracted by Gastrointestinal Endoscopy from Health Check-up Patients in Korea Hyemi Song, Bong-Kwang Jung, Jaeeun Cho, Taehee Chang, Sun Huh, Jong-Yil Chai The Korean Journal of Parasitology.2019; 57(2): 207. CrossRef What Are the Clinical Features and Etiology of Eosinophilic Liver Infiltration? Da Woon Sim, Dong Jun Son, Eunae Cho, Sung Kyu Choi, Sang Soo Shin, Chung Hwan Jun Gut and Liver.2019; 13(2): 183. CrossRef Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea Eun Jeong Won, Ju Hyeon Shin, Yu Jeong Lee, Moon-Ju Kim, Seung Ji Kang, Sook In Jung, Soo Hyun Kim, Jong Hee Shin, Jong-Yil Chai, Sung-Shik Shin The Korean Journal of Parasitology.2019; 57(3): 313. CrossRef Anisakid Larvae from Anchovies in the South Coast of Korea Taehee Chang, Bong-Kwang Jung, Sooji Hong, Hyejoo Shin, Jeonggyu Lee, Laddawan Patarwut, Jong-Yil Chai The Korean Journal of Parasitology.2019; 57(6): 699. CrossRef In Vivo Endoscopic Removal of Ancylostoma duodenale in a Patient with Abdominal Pain Soo-Young Na, Heung Up Kim, Young Ree Kim, Seung Uk Jeong, Sun-Jin Boo, Hyun Joo Song, Eun Kwang Choi, Byung-Cheol Song The Korean Journal of Helicobacter and Upper Gastrointestinal Research.2018; 18(1): 61. CrossRef Cysticercosis/taeniasis endemicity in Southeast Asia: Current status and control measures Hai-Wei Wu, Akira Ito, Lin Ai, Xiao-Nong Zhou, Luz P. Acosta, Arve Lee Willingham III Acta Tropica.2017; 165: 121. CrossRef Anisakiasis: Report of 15 Gastric Cases Caused by <i>Anisakis</i> Type I Larvae and a Brief Review of Korean Anisakiasis Cases Woon-Mok Sohn, Byoung-Kuk Na, Tae Hyo Kim, Tae-Joon Park The Korean Journal of Parasitology.2015; 53(4): 465. CrossRef A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient Ivona Mladineo, Marijana Popović, Irena Drmić-Hofman, Vedran Poljak BMC Infectious Diseases.2015;[Epub] CrossRef Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil Cláudio Mafra, Cynthia Mantovani, Juliana Novo Borges, Rafael Mazioli Barcelos, Cláudia Portes Santos Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 209. CrossRef Anisakids (Nematoda: Anisakidae) from stomachs of northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska: parasitological and pathological analysis T. A. Kuzmina, E. T. Lyons, T. R. Spraker Parasitology Research.2014; 113(12): 4463. CrossRef 11,501 View 127 Download 14 Web of Science Crossref Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis Hyun-Ouk Song, Jae-Sook Ryu Korean J Parasitol 2013;51(4):479-484. Published online August 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.4.479 Abstract PDF Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O₂^.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis. Citations Citations to this article as recorded by IDO1 promotes Echinococcus multilocularis infection by regulating the formation of neutrophil extracellular traps Haining Zhang, Ru Meng, Fan Zhang, Ao Chen, Hongrun Ge, Wangkai Chen, Zhi Li, Yong Fu Veterinary Research.2025;[Epub] CrossRef Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila Parasitology Research.2022; 121(5): 1355. CrossRef Immunological Activities of Crude Polysaccharides Extracts ofClimacodon pulcherrimus(Phanerochaetaceae) in Lipopolysaccharide-Induced Rat Macrophages and Neutrophils' Responses Tume Christopher, Oumar Mahamat, Miyanui N. Chungong, C. Atemenkeh Ngwa, P. Maishu Samka Journal of Medicinal Food.2021; 24(2): 180. CrossRef Angiotensin‐converting enzyme as a new immunologic target for the new SARS‐CoV‐2 Shahin Hallaj, Anahita Ghorbani, Seyed Ali Mousavi‐Aghdas, Mohammad Mirza‐Aghazadeh‐Attari, Andrey Sevbitov, Vida Hashemi, Tooba Hallaj, Farhad Jadidi‐Niaragh Immunology & Cell Biology.2021; 99(2): 192. CrossRef The state of art of neutrophil extracellular traps in protozoan and helminthic infections César Díaz-Godínez, Julio C. Carrero Bioscience Reports.2019;[Epub] CrossRef Recombinant α-actinin subunit antigens of Trichomonas vaginalis as potential vaccine candidates in protecting against trichomoniasis Yi-Ting Xie, Jiang-Mei Gao, Ya-Ping Wu, Petrus Tang, Geoff Hide, De-Hua Lai, Zhao-Rong Lun Parasites & Vectors.2017;[Epub] CrossRef Adenosine reduces reactive oxygen species and interleukin-8 production by Trichomonas vaginalis-stimulated neutrophils Amanda Piccoli Frasson, Camila Braz Menezes, Gustavo Krumel Goelzer, Simone Cristina Baggio Gnoatto, Solange Cristina Garcia, Tiana Tasca Purinergic Signalling.2017; 13(4): 569. CrossRef 9,462 View 71 Download Crossref Proliferation of Toxoplasma gondii Suppresses Host Cell Autophagy Youn-Jin Lee, Hyun-Ouk Song, Young-Ha Lee, Jae-Sook Ryu, Myoung-Hee Ahn Korean J Parasitol 2013;51(3):279-287. Published online June 30, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.3.279 Abstract PDF Autophagy is a process of cytoplasmic degradation of endogenous proteins and organelles. Although its primary role is protective, it can also contribute to cell death. Recently, autophagy was found to play a role in the activation of host defense against intracellular pathogens. The aims of our study was to investigate whether host cell autophagy influences Toxoplasma gondii proliferation and whether autophagy inhibitors modulate cell survival. HeLa cells were infected with T. gondii with and without rapamycin treatment to induce autophagy. Lactate dehydrogenase assays showed that cell death was extensive at 36-48 hr after infection in cells treated with T. gondii with or without rapamycin. The autophagic markers, LC3 II and Beclin 1, were strongly expressed at 18-24 hr after exposure as shown by Western blotting and RT-PCR. However, the subsequent T. gondii proliferation suppressed autophagy at 36 hr post-infection. Pre-treatment with the autophagy inhibitor, 3-methyladenine (3-MA), down-regulated LC3 II and Beclin 1. The latter was also down-regulated by calpeptin, a calpain inhibitor. Monodansyl cadaverine (MDC) staining detected numerous autophagic vacuoles (AVs) at 18 hr post-infection. Ultrastructural observations showed T. gondii proliferation in parasitophorous vacuoles (PVs) coinciding with a decline in the numbers of AVs by 18 hr. FACS analysis failed to confirm the presence of cell apoptosis after exposure to T. gondii and rapamycin. We concluded that T. gondii proliferation may inhibit host cell autophagy and has an impact on cell survival. Citations Citations to this article as recorded by Modulation of autophagy as a therapeutic strategy for Toxoplasma gondii infection Ao Cheng, Huanan Zhang, Baike Chen, Shengyao Zheng, Hongyi Wang, Yijia Shi, Siyao You, Ming Li, Liping Jiang Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef Myrislignan Induces Redox Imbalance and Activates Autophagy in Toxoplasma gondii Jili Zhang, Jia Chen, Kun Lv, Bing Li, Biqing Yan, Lei Gai, Chaolu Shi, Xinnian Wang, Hongfei Si, Jiyu Zhang Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef 4-Hydroxybenzaldehyde Restricts the Intracellular Growth of Toxoplasma gondii by Inducing SIRT1-Mediated Autophagy in Macrophages Jina lee, Jae-Won Choi, Hye Young Han, Woo Sik Kim, Ha-Yeon Song, Eui-Baek Byun, Eui-Hong Byun, Young-Ha Lee, Jae-Min Yuk The Korean Journal of Parasitology.2020; 58(1): 7. CrossRef Foot-and-mouth disease virus induces PERK mediated autophagy to suppress antiviral interferon response H. B. Ranjitha, Veena Ammanathan, Neha Guleria, Madhusudan Hosamani, B. P. Sreenivasa, V. V. Dhanesh, Rashmi Santhoshkumar, B. K. Chandrasekhar Sagar, B. P. Mishra, R. K. Singh, Aniket Sanyal, Ravi Manjithaya, Suresh H. Basagoudanavar Journal of Cell Science.2020;[Epub] CrossRef The Nrf2 pathway is required for intracellular replication ofToxoplasma gondiiin activated macrophages Yu Pang, Zhaoxia Zhang, Yuntong Chen, Shinuo Cao, Xiuqin Yang, Honglin Jia Parasite Immunology.2019;[Epub] CrossRef Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt Frontiers in Immunology.2017;[Epub] CrossRef Anti-Toxoplasmosis Effect of the Herbal Extracts Plantago asiatica L. Sunhwa Hong, Okjin Kim Korean Journal of Plant Resources.2016; 29(1): 26. CrossRef Autophagy activated by Toxoplasma gondii infection in turn facilitates Toxoplasma gondii proliferation Dongmei Gao, Jing Zhang, Jun Zhao, He Wen, Jiwen Pan, Shouzhu Zhang, Yong Fang, Xiuyi Li, Yu Cai, Xuelong Wang, Shiping Wang Parasitology Research.2014; 113(6): 2053. CrossRef Anti-Toxoplasmosis Effect of the Halophyte Suaeda maritime Sunhwa Hong, Hyun-A Lee, Yun-Seong Lee, Dong-Woo Kim, Jae-Hyeok Jeong, Tae-Wan Kim, Okjin Kim Korean Journal of Plant Resources.2014; 27(5): 415. CrossRef 12,000 View 123 Download Crossref The Dimension of Trichomonas vaginalis as Measured by Scanning Electron Microscopy Sang-Hoon Cheon, Seung Ryong Kim, Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu Korean J Parasitol 2013;51(2):243-246. Published online April 25, 2013 DOI: https://doi.org/10.3347/kjp.2013.51.2.243 Abstract PDF It is known that physicochemical conditions (e.g., pH, temperature, and ionic strength) affect the size of trichomonads. In this study, the sizes of 4 isolates of Trichomonas vaginalis cultured for more than a year (called "old T") and 3 isolates freshly isolated from vaginitis cases (called "fresh T") were compared by scanning electron microscopy. Although the fresh T had shorter body length, body width, and flagellar length than old T, total length (about 26 ?m), including body length, flagella length, and axostyle length was almost the same in the 2 groups. A striking difference was observed between the axostyles of the 2 groups; the axostyle length of the fresh T (8.2 ?m) was more than twice as long as that of the old T (4.0 ?m). However, in several parasitology textbooks, the length of T. vaginalis is said to vary widely from 7 to 32 ?m, and its undulating membrane is said to extend about half way (53.5%) to the posterior end of the body. On the other hand, in our study, the undulating membrane was observed to extend more than 3/4 of the body length (72.1%) in old T, whereas in fresh T it could not be measured. Taken together, we suggest that T. vaginalis averages 26 (21-32) ?m in total length, with 9.5 (7.4-11.4) ?m of body length and 6.8 (5.3-7.7) ?m of width, and its undulating membrane extending 3/4 of its body length. Therefore, these findings may provide useful information for morphological characteristics of T. vaginalis. Citations Citations to this article as recorded by In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis Fernanda Gomes Cardoso, Luisa Trindade dos Santos, Saulo Almeida Menezes, Graziela Vargas Rigo, Tiana Tasca Frontiers in Parasitology.2025;[Epub] CrossRef Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef Neutrophil interactions with the sexually transmitted parasite Trichomonas vaginalis: implications for immunity and pathogenesis Suhani B. Bhakta, Jose A. Moran, Frances Mercer Open Biology.2020;[Epub] CrossRef Neutrophils kill the parasite Trichomonas vaginalis using trogocytosis Frances Mercer, Shek Hang Ng, Taylor M. Brown, Grace Boatman, Patricia J. Johnson, Fred Hughson PLOS Biology.2018; 16(2): e2003885. CrossRef Prostatic Disease Associated withTrichomonas vaginalis Jae-Sook Ryu The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef 11,386 View 86 Download Crossref Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate Hyun-Ouk Song, Young-Su Lim, Sun-Joo Moon, Myoung-Hee Ahn, Jae-Sook Ryu Korean J Parasitol 2010;48(1):1-7. Published online March 17, 2010 DOI: https://doi.org/10.3347/kjp.2010.48.1.1 Abstract PDF Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis. Citations Citations to this article as recorded by The role of neutrophils in the pathogenesis of Trichomonas vaginalis infection in pregnant women: A review Emmanuel Ifeanyi Obeagu, Gideon Ikechukwu Okoroiwu, Nwanganga Ihuoma Ubosi, Getrude Uzoma Obeagu, Ebere Emilia Ayogu, Elham Elamin Medicine.2025; 104(41): e45063. CrossRef Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei Acta Tropica.2023; 238: 106794. CrossRef Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila Parasitology Research.2022; 121(5): 1355. CrossRef Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis F. J. Rangel-Mata, E. E. Ávila-Muro, J. E. Reyes-Martínez, L. M. Olmos-Ortiz, M. E. Brunck, L. A. Arriaga-Pizano, P. Cuéllar-Mata Parasitology.2021; 148(13): 1624. CrossRef Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells Fei Fei Gao, Juan-Hua Quan, Min A. Lee, Wei Ye, Jae-Min Yuk, Guang-Ho Cha, In-Wook Choi, Young-Ha Lee Parasites & Vectors.2021;[Epub] CrossRef Molecular investigation of Trichomoniasis in women in Al-Muthana province /Iraq H R J Al-abodi, K T M Al-Shaibani, E M Shaker Journal of Physics: Conference Series.2019; 1234(1): 012078. CrossRef Amaurocine: Anti- Trichomonas vaginalis protein produced by the basidiomycete Amauroderma camerarium Mariana Duarte, Adriana Seixas, Maira Peres de Carvalho, Tiana Tasca, Alexandre José Macedo Experimental Parasitology.2016; 161: 6. CrossRef Trichomonas vaginalis induces cytopathic effect on human lung alveolar basal carcinoma epithelial cell line A549 Daile Meek C. Salvador-Membreve, Sonia D. Jacinto, Windell L. Rivera Experimental Parasitology.2014; 147: 33. CrossRef Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes Juan-Hua Quan, Byung-Hun Kang, Guang-Ho Cha, Wei Zhou, Young-Bok Koh, Jung-Bo Yang, Heon-Jong Yoo, Min-A Lee, Jae-Sook Ryu, Heung-Tae Noh, Jaeyul Kwon, Young-Ha Lee, A. R. M. Ruhul Amin PLoS ONE.2014; 9(10): e110659. CrossRef Altered CD16 expression on vaginal neutrophils from women with vaginitis Joziani Beghini, Paulo C. Giraldo, Ruth Riboldi, Rose L.G. Amaral, José Eleutério, Steven S. Witkin, Fernando Guimarães European Journal of Obstetrics & Gynecology and Reproductive Biology.2013; 167(1): 96. CrossRef Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin Inflammation Research.2012; 61(2): 97. CrossRef NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced byTrichomonas vaginalis-Derived Secretory Products Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin The Korean Journal of Parasitology.2011; 49(3): 291. CrossRef Trichomoniasis Jae-Sook Ryu Hanyang Medical Reviews.2010; 30(3): 213. CrossRef 10,075 View 94 Download Crossref Indochinamon ou (Crustacea: Potamidae) as a New Second Intermediate Host for Paragonimus harinasutai in Luang Prabang Province, Lao PDR Woon-Mok Sohn, Jae-Sook Ryu, Duk-Young Min, Hyun-Ouk Song, Han-Jong Rim, Youthanavanh Vonghachack, Daluny Bouakhasith, Virasack Banouvong Korean J Parasitol 2009;47(1):25-29. Published online March 12, 2009 DOI: https://doi.org/10.3347/kjp.2009.47.1.25 Abstract PDF Paragonimus harinasutai metacercariae were found in a species of freshwater crab, Indochinamon ou, collected in a small stream of Namback District, Luang Prabang Province, Lao PDR. Adult flukes were recovered after experimental infection of the metacercariae to dogs. Metacercariae were round or slightly elliptical, 0.666 × 0.626 mm in average size, and had a thin cyst wall of about 20 ?m in thickness, a black excretory bladder, convoluted ceca, and some pinkish materials in the body. Adults were somewhat elongated, 95.2 × 36.5 mm in average size, covered with single-tipped tegumental spines, had a smaller oral sucker than the ventral sucker, a moderately branched ovary, and 5-6 lobulated testes. Eggs were ovoid and bilaterally symmetrical in shape, 79 × 45 ?m in average size, and had a uniformly thickened shell. By the present study, it has been confirmed that I. ou is a new second intermediate host for P. harinasutai. Citations Citations to this article as recorded by Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool Infection, Genetics and Evolution.2025; 128: 105718. CrossRef Usefulness and limits of Ziehl-Neelsen staining to detect paragonimiasis in highly endemic tuberculosis areas Kethmany Ratsavong, Fabrice Quet, Fulgence Nzabintwali, Jeoffray Diendéré, Jacques Sebert, Michel Strobel, Yves Buisson Parasite Epidemiology and Control.2017; 2(1): 1. CrossRef Morphological and molecular identification of a lung fluke, Paragonimus macrorchis (Trematoda, Paragonimidae), found in central Lao PDR and its molecular phylogenetic status in the genus Paragonimus Oranuch Sanpool, Pewpan Maleewong Intapan, Tongjit Thanchomnang, Penchom Janwan, Sakhone Laymanivong, Hiromu Sugiyama, Wanchai Maleewong Parasitology International.2015; 64(6): 513. CrossRef 10,567 View 108 Download Crossref Skin Test for Paragonimiasis among Schoolchildren and Villagers in Namback District, Luangprabang Province, Lao PDR Hyun-Ouk Song, Duk-Young Min, Han-Jong Rim, Vonghachack Youthanavanh, Bouakhasith Daluny, Vongsouvan Sengdara, Banouvong Virasack, Phommasak Bounlay Korean J Parasitol 2008;46(3):179-182. Published online September 20, 2008 DOI: https://doi.org/10.3347/kjp.2008.46.3.179 Abstract PDF As a part of a broader effort to determine the status of Paragonimus species infection in Lao PDR, an epidemiological survey was conducted on villagers and schoolchildren in Namback District between 2003 and 2005. Among 308 villagers and 633 primary and secondary schoolchildren, 156 villagers and 92 children evidenced a positive reaction on a Paragonimus skin test. Only 4 schoolchildren out of 128 skin test-positive cases had Paragonimus sp. eggs in their sputum, all of which was collected on 1 day. Several types of crabs, which were identified as the second intermediate host of the Paragonimus species, were collected from markets and streams in a paragonimiasis endemic area for the inspection of metacercariae. Among the examined crabs, only "rock crabs" (Indochinamon ou) harbored Paragonimus sp. metacercariae, and it is speculated that the life cycle of Paragonimus sp. was maintained via rock crabs in Namback District, Lao PDR. Citations Citations to this article as recorded by Paragonimus and paragonimiasis in Asia: An update Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama Acta Tropica.2019; 199: 105074. CrossRef Usefulness and limits of Ziehl-Neelsen staining to detect paragonimiasis in highly endemic tuberculosis areas Kethmany Ratsavong, Fabrice Quet, Fulgence Nzabintwali, Jeoffray Diendéré, Jacques Sebert, Michel Strobel, Yves Buisson Parasite Epidemiology and Control.2017; 2(1): 1. CrossRef 7,922 View 81 Download Crossref Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate Eun-Jung Lee, Yoo-Mi Heo, Jong-Hak Choi, Hyun-Ouk Song, Jae-Sook Ryu, Myoung-Hee Ahn Korean J Parasitol 2008;46(3):145-151. Published online September 20, 2008 DOI: https://doi.org/10.3347/kjp.2008.46.3.145 Abstract PDF During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of pro- and anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-α, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-α expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. 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Published online December 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.4.373 Abstract PDF To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in iron-depleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC₆ also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. 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CrossRef 9,265 View 113 Download Crossref Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii Ji-Young Kim, Myoung-Hee Ahn, Hyun-Ouk Song, Jong-Hak Choi, Jae-Sook Ryu, Duk-Young Min, Myung-Hwan Cho Korean J Parasitol 2006;44(3):197-207. Published online September 20, 2006 DOI: https://doi.org/10.3347/kjp.2006.44.3.197 Abstract PDF This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and MIP-1α, and enzyme, COX-2/prostaglandin E₂ (PGE₂) in infected cells via western blot, [³H]-uracil incorporation assay, ELISA and RT-PCR. The phosphorylation of ERK1/2 and p38 in infected HeLa cells was detected at 1 hr and/or 6 hr postinfection (PI). Tachyzoite proliferation was reduced by p38 or JNK MAPK inhibitors. MCP-1 secretion was enhanced in infected peritoneal macrophages at 6 hr PI. MIP-1α mRNA was increased in macrophages at 18 hr PI. MCP-1 and MIP-1α were reduced after treatment with inhibitors of ERK1/2 and JNK MAPKs. COX-2 mRNA gradually increased in infected RAW 264.7 cells and the secretion of COX-2 peaked at 6 hr PI. The inhibitor of JNK suppressed COX-2 expression. PGE₂ from infected RAW 264.7 cells was increased and synthesis was suppressed by PD98059, SB203580, and SP600125. In this study, the activation of p38, JNK and/or ERK1/2 MAPKs occurred during the invasion and proliferation of T. gondii tachyzoites in HeLa cells. Also, increased secretion and expression of MCP-1, MIP-1α, COX-2 and PGE₂ were detected in infected macrophages, and appeared to occur via MAPK signaling pathways. Citations Citations to this article as recorded by Differential Expression of hsa‐miR‐144‐3p and hsa‐miR‐125b‐5p in Gestational Toxoplasmosis Ingrid de Siqueira Pereira, Mariana Ramire Cortez, Tamires Santos de Arruda, Francieli Marinho Carneiro, Allecineia Bispo da Cruz, Ricardo Gava, Geraldo Magela de Faria Junior, Ingrid Gomes de Campos Truzzi, Lígia Cosentino Junqueira Franco Spegiorin, San Parasite Immunology.2025;[Epub] CrossRef Qualitative analysis of Fasciola gigantica excretory and secretory products coimmunoprecipitated with buffalo secondary infection sera shows dissimilar components from primary infection sera Mengwei Zheng, Xinping Kong, Xuelian Jiang, Yankun Yang, Shishi Fu, Chongli Wen, Weiyu Zhang, Wenda Di Acta Tropica.2024; 260: 107391. CrossRef The roles of COX-2 in protozoan infection Xinlei Wang, Jie Chen, Jingtong Zheng Frontiers in Immunology.2023;[Epub] CrossRef Immunomodulatory effects ofInonotus obliquuspolysaccharide on splenic lymphocytes infected withToxoplasma gondiivia NF-κB and MAPKs pathways Rui Sang, Fuliang Sun, Hongyuan Zhou, Meng Wang, Haitao Li, Chunting Li, Xinhui Sun, Xin Zhao, Xuemei Zhang Immunopharmacology and Immunotoxicology.2022; 44(1): 129. CrossRef Inhibitory Effects of Inonotus obliquus Polysaccharide on Inflammatory Response in Toxoplasma gondii-Infected RAW264.7 Macrophages Kexin Yan, Hongyuan Zhou, Meng Wang, Haitao Li, Rui Sang, Bingjie Ge, Xin Zhao, Chunting Li, Wei Wang, Xuemei Zhang, Angelica Oliveira Gomes Evidence-Based Complementary and Alternative Medicine.2021; 2021: 1. CrossRef Regulation of Proinflammatory Enzymes by Peroxisome Proliferator–Activated Receptor Gamma in Astroglia Infected with Toxoplasma gondii Ling-Yuh Shyu, Ke-Min Chen, Cheng-You Lu, Shih-Chan Lai Journal of Parasitology.2020; 106(5): 564. CrossRef Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2 Iliana Claudia Balga Milian, Rafaela José Silva, Camilla Manzan-Martins, Bellisa Freitas Barbosa, Pamela Mendonça Guirelli, Mayara Ribeiro, Angelica de Oliveira Gomes, Francesca Ietta, José Roberto Mineo, Priscila Silva Franco, Eloisa Amália Vieira Ferro Frontiers in Microbiology.2019;[Epub] CrossRef Cyclooxygenase (COX)-2 Inhibitors Reduce Toxoplasma gondii Infection and Upregulate the Pro-inflammatory Immune Response in Calomys callosus Rodents and Human Monocyte Cell Line Ana Carolina Alcântara Pereira, Rafaela José Silva, Priscila Silva Franco, Angelica de Oliveira Gomes, Guilherme Souza, Iliana Claudia Balga Milian, Mayara Ribeiro, Alessandra Monteiro Rosini, Pâmela Mendonça Guirelli, Eliézer Lucas Pires Ramos, Tiago Wil Frontiers in Microbiology.2019;[Epub] CrossRef A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18 Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou Molecular & Cellular Proteomics.2017; 16(3): 469. 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CrossRef 10,179 View 90 Download Crossref Influence of calcium ion on host cell invasion and intracellular replication by Toxoplasma gondii Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu, Duk-Young Min, Kyoung-Hwan Joo, Young-Ha Lee Korean J Parasitol 2004;42(4):185-193. Published online December 20, 2004 DOI: https://doi.org/10.3347/kjp.2004.42.4.185 Abstract PDF Toxoplasma gondii is an obligate intracellular protozoan parasite, which invades a wide range of hosts including humans. The exact mechanisms involved in its invasion are not fully understood. This study focused on the roles of Ca²⁺ in host cell invasion and in T. gondii replication. We examined the invasion and replication of T. gondii pretreated with several calcium modulators, the conoid extrusion of tachyzoites. Calmodulin localization in T. gondii were observed using the immunogold method, and Ca²⁺ levels in tachyzoites by confocal microscopy. In light microscopic observation, tachyzoites co-treated with A23187 and EGTA showed that host cell invasion and intracellular replication were decreased. The invasion of tachyzoites was slightly inhibited by the Ca²⁺ channel blockers, bepridiland verapamil, and by the calmodulin antagonist, calmidazolium. We observed that calcium saline containing A23187 induced the extrusion of tachyzoite conoid. By immunoelectron microscopy, gold particles bound to anti-calmodulin or anti-actin mAb, were found to be localized on the anterior portion of tachyzoites. Remarkably reduced intracellular Ca²⁺ was observed in tachyzoites treated with BAPTA/AM by confocal microscopy. These results suggest that host cell invasion and the intracellular replication of T. gondii tachyzoites are inhibited by the calcium ionophore, A23187, and by the extracellular calcium chelator, EGTA. Citations Citations to this article as recorded by Unveiling the impact of cGMP-dependent protein kinase of Neospora caninum on calcium fluxes and egress functions through quantitative phosphoproteome analysis Xianmei Wang, Kun Guo, Zhili Shan, Zhu Ying, Zifu Zhu, Shiman Yang, Na Yang, Qun Liu, Lifang Wang, Jing Liu Communications Biology.2025;[Epub] CrossRef Calreticulin (CALR) promotes ionophore-induced microneme secretion in Toxoplasma gondii Zhili Shan, Xingju Song, Xu Yang, Yangfei Xue, Yayun Wu, Xianmei Wang, Jing Liu, Qun Liu Parasitology Research.2024;[Epub] CrossRef Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration Hyunho Kim, Sung-Hee Hong, Hyo Eun Jeong, Sewoon Han, Jinchul Ahn, Jin-A. 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