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Myoung Ae Park	2 Articles
Molecular Identification and Real-time Quantitative PCR (qPCR) for Rapid Detection of Thelohanellus kitauei, a Myxozoan Parasite Causing Intestinal Giant Cystic Disease in the Israel Carp Jung Soo Seo, Eun Ji Jeon, Moo Sang Kim, Sung Ho Woo, Jin Do Kim, Sung Hee Jung, Myoung Ae Park, Bo Young Jee, Jin Woo Kim, Yi-Cheong Kim, Eun Hye Lee Korean J Parasitol 2012;50(2):103-111. Published online May 24, 2012 DOI: https://doi.org/10.3347/kjp.2012.50.2.103 Abstract PDF Intestinal giant-cystic disease (IGCD) of the Israel carp (Cyprinus carpio nudus) has been recognized as one of the most serious diseases afflicting inland farmed fish in the Republic of Korea, and Thelohanellus kitauei has been identified as the causative agent of the disease. Until now, studies concerning IGCD caused by T. kitauei in the Israel carp have been limited to morphological and histopathological examinations. However, these types of diagnostic examinations are relatively time-consuming, and the infection frequently cannot be detected in its early stages. In this study, we cloned the full-length 18S rRNA gene of T. kitauei isolated from diseased Israel carps, and carried out molecular identification by comparing the sequence with those of other myxosporeans. Moreover, conventional PCR and real-time quantitative PCR (qPCR) using oligonucleotide primers for the amplification of 18S rRNA gene fragment were established for further use as methods for rapid diagnosis of IGCD. Our results demonstrated that both the conventional PCR and real-time quantitative PCR systems applied herein are effective for rapid detection of T. kitauei spores in fish tissues and environmental water. Citations Citations to this article as recorded by Molecular phylogenetics reveals a species complex pattern of closely related members of genus Thelohanellus (Cnidaria: Myxosporea) from the Indian subcontinent Harpreet Kaur, Aditya Gupta, Rajni Attri Microbial Pathogenesis.2021; 150: 104690. CrossRef Potential Application of PCR Based Molecular Methods in Fish Pathogen Identification: A Review Md. Ali Arman Ador, Md. Shameul Haque, Sulav Indra Paul, Jui Chakma, Rakib Ehsan, Ashikur Rahman Aquaculture Studies.2021;[Epub] CrossRef A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea Sang Phil Shin, Chang Nam Jin, Han Chang Sohn, Hiroshi Yokoyama, Jehee Lee Parasitology Research.2019; 118(11): 3105. CrossRef Development of PCR method for detecting Kudoa iwatai (Myxozoa: Multivalvulida) from rock bream Oplegnathus fasciatus Chan-Hyeok Jeon, Jeung-Wan Do, U-Hwa Nam, Wi-Sik Kim, Jeong-Ho Kim Parasitology Research.2017; 116(2): 789. CrossRef Molecular identification of a new myxozoan, Myxobolus dermiscalis n. sp. (Myxosporea) infecting scales of Labeo rohita Hamilton in Harike Wetland, Punjab (India) Harpreet Kaur, Rajni Attri, Jyoti Joshi International Journal for Parasitology: Parasites and Wildlife.2016; 5(2): 139. CrossRef The life cycle of Thelohanellus kitauei (Myxozoa: Myxosporea) infecting common carp (Cyprinus carpio) involves aurantiactinomyxon in Branchiura sowerbyi Dandan Zhao, Muhammad Hafiz Borkhanuddin, Weimin Wang, Yang Liu, Gábor Cech, Yanhua Zhai, Csaba Székely Parasitology Research.2016; 115(11): 4317. CrossRef Genetic relatedness provides support for a species complex of myxosporeans infecting the Indian major carp, Labeo rohita Harpreet Kaur, Aditya Gupta Animal Biology.2015; 65(3-4): 337. CrossRef Morphology and Phylogeny of Thelohanellus marginatus n. sp. (Myxozoa: Myxosporea), a Parasite Infecting the Gills of the Fish Hypophthalmus marginatus (Teleostei: Pimelodidae) in the Amazon River Sónia Rocha, Graça Casal, Michele Velasco, Ângela Alves, Edilson Matos, Saleh Al‐Quraishy, Carlos Azevedo Journal of Eukaryotic Microbiology.2014; 61(6): 586. CrossRef The phylogenetic study on Thelohanellus species (Myxosporea) in relation to host specificity and infection site tropism Sang Phil Shin, Van Giap Nguyen, Jae Mook Jeong, Jin Woo Jun, Ji Hyung Kim, Jee Eun Han, Gun Wook Baeck, Se Chang Park Molecular Phylogenetics and Evolution.2014; 72: 31. CrossRef New phylogenomic and comparative analyses provide corroborating evidence that Myxozoa is Cnidaria Jin-Mei Feng, Jie Xiong, Jin-Yong Zhang, Ya-Lin Yang, Bin Yao, Zhi-Gang Zhou, Wei Miao Molecular Phylogenetics and Evolution.2014; 81: 10. CrossRef Discrimination and simultaneous detection of two myxozoan parasites belonging to genus Thelohanellus by multiplex polymerase chain reaction Sung Ho Woo, Jung Soo Seo, Eun Hye Lee Veterinary Parasitology.2014; 203(1-2): 212. CrossRef Understanding myxozoan infection dynamics in the sea: Seasonality and transmission of Ceratomyxa puntazzi Gema Alama-Bermejo, Radek Šíma, Juan A. Raga, Astrid S. Holzer International Journal for Parasitology.2013; 43(9): 771. CrossRef 11,787 View 81 Download Crossref Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study Hee Jung Choi, Jee Youn Hwang, Dong Lim Choi, Min Do Huh, Young Baek Hur, Nam-Sil Lee, Jung Soo Seo, Mun Gyeong Kwon, Hye-Sung Choi, Myoung Ae Park Korean J Parasitol 2011;49(3):229-234. Published online September 30, 2011 DOI: https://doi.org/10.3347/kjp.2011.49.3.229 Abstract PDF In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas. Citations Citations to this article as recorded by Integrated immune responses in Manila clam: Antimicrobial activity, immune-related enzymes activity and gene expression profiles following Vibrio anguillarum challenge Yongchao Li, Tianshuo Huang, Pengxiang Lin, Jie Yu, Hongtao Nie Fish & Shellfish Immunology.2026; 171: 111166. CrossRef Transcriptome-wide identification, functional characterization, and expression analysis of two novel invertebrate-type Toll-like receptors from disk abalone (Haliotis discus discus) Thanthrige Thiunuwan Priyathilaka, S.D.N.K. Bathige, Seongdo Lee, Bo-Hye Nam, Jehee Lee Fish & Shellfish Immunology.2019; 84: 802. CrossRef The dynamics of total organic matter (tom) on sangkuriang catfish (clarias gariepinus) farming at upt ptpbp2kp and the effectiveness of freshwater bivalve (anodonta woodiana) in reducing the total organic matter with varying density D Arfiati, C D G Putra, A H Tullah, S W A Permanasari, A W Puspitasari IOP Conference Series: Earth and Environmental Science.2019; 236: 012022. CrossRef The role of copper and zinc accumulation in defense against bacterial pathogen in the fujian oyster (Crassostrea angulata) Bo Shi, Tian Wang, Zhen Zeng, Long Zhou, Weiwei You, Caihuan Ke Fish & Shellfish Immunology.2019; 92: 72. CrossRef Hybridization improved bacteria resistance in abalone: Evidence from physiological and molecular responses Shuang Liang, Xuan Luo, Weiwei You, Caihuan Ke Fish & Shellfish Immunology.2018; 72: 679. CrossRef Morphofunctional parameters of hemocytes in the assessment of the physiological status of bivalves A. A. Anisimova Russian Journal of Marine Biology.2013; 39(6): 381. CrossRef Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil) Fernando Ramos Queiroga, Luis Fernando Marques-Santos, Hélène Hégaret, Philippe Soudant, Natanael Dantas Farias, Aline Daiane Schlindwein, Patricia Mirella da Silva Fish & Shellfish Immunology.2013; 35(2): 319. CrossRef Relative contribution of reproductive investment, thermal stress and Vibrio infection to summer mortality phenomena in Pacific oysters Carolin C. Wendling, K. Mathias Wegner Aquaculture.2013; 412-413: 88. CrossRef Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity Yang Zhang, Xiaocui He, Feng Yu, Zhiming Xiang, Jun Li, Karen L. Thorpe, Ziniu Yu, Irving Coy Allen PLoS ONE.2013; 8(10): e76464. CrossRef 10,307 View 89 Download Crossref