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Original Article

Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study

Hee Jung Choi1, Jee Youn Hwang1, Dong Lim Choi1, Min Do Huh2, Young Baek Hur3, Nam-Sil Lee1, Jung Soo Seo1, Mun Gyeong Kwon1, Hye-Sung Choi1, Myoung Ae Park1
The Korean Journal of Parasitology 2011;49(3):229-234.
Published online: September 30, 2011

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Corresponding author (jyhwang@nfrdi.go.kr)
• Received: March 10, 2011   • Revised: June 8, 2011   • Accepted: June 17, 2011

© 2011, Korean Society for Parasitology

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  • In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.
  • Key words: Marteilioides chungmuensis, pacific oyster, Crassostrea gigas, flow cytometry, hemocyte
The aquaculture production of Crassostrea gigas in Korea has increased markedly, from more than 40,000 tons in the 1960s to more than 960,000 tons in 1973, following the introduction of aquaculture. Since the 1990s, however, this pursuit has experienced difficulties due to poor natural seed collection [1]. The causes of this poor natural seed collection have been investigated and identified. Infections with Marteilioides chungmuensis, a paramyxean protozoa affecting the normal oviposition and development of fertilized eggs and impairing the product quality by damaging the visual attractiveness have been identified as causative factors in the reduced aquaculture production of oysters, C. gigas [2]. Studies of this ovarian parasite included the assessment of microstructures [3], process of spore formation [4], initial infection route[5], rate of infection [2,4,6,7], development of diagnostic methods [8,9], pathogenicity [2,9], biochemical examinations [6], and effects of environmental factors [10,11]. A variety of such studies have been conducted in Korea and Japan. Thus far, however, there have been no studies which characterized the mechanisms underlying host defenses against M. chungmuensis infection.
In the bivalvia, hemocytes perform activities, such as encapsulation, phagocytosis, inflammation, and wound healing for the removal of foreign bodies infiltrating the body. Thus, they perform a key role in self-defense mechanisms [12,13]. In recent years, flow cytometry has been extensively used to assess the mechanisms by which the marine bivalves exert a cellular defensive effect. This has been shown to be useful, prompt, and accurate in the quantitative analysis of the morphology and functions of each cell. Thus, this technique has been very effectively employed. To date, by employing these methods, the immune parameters of marine bivalvia, including phagocytosis, total hemocyte counts, respiratory burst, and hemocyte mortality, have been previously assessed [14,15].
Given the background outlined above, we have conducted this study to evaluate the effects of M. chungmuensis infection in C. gigas. To accomplish this, we assessed the alterations of immunecompetency factors as seen by flow cytometry in the hemocytes of oysters infected with this ovarian parasite, as well as those in healthy appearing oysters.
Sampling
The Pacific oysters, Crassostrea gigas, used in this study were collected 3 times from an oyster bed in Goseong-gun, Gyeongsangnam-do in August 2009. The oysters were then transferred to our laboratory and used for the following experimental procedures. Oysters were divided into M. chungmuensis-infected group (Fig. 1), in which nodules were grossly formed on the mantle after removal of the shell, and healthy appearing oysters (HAO) group, where healthy findings were grossly present. After removing the shell, hemolymph was extracted from the heart with a 1-ml syringe. After extraction, hemocytes were maintained on ice to inhibit hemocyte clumping until they were needed for the experiments [16]. At the first experimental session, 18 HAO were divided into 3 groups and infected oysters (n=4) were used as 1 group. At the second session, 36 HAO were divided into 6 groups and the infected oysters (n=10) were divided into 3 groups. At the third experimental session, 36 HAO were divided 6 groups and the infected oysters (n=12) were divided into 4 groups. The hemolymph was combined into pools of oysters each to reduce individual variation.
Flow cytometric analyses of oyster hemocytes
In analysis of the extracted hemolymph, a flow cytometer was initialized with an autocomp using a single laser FACScan system (Becton-Dickinson, San Jose, California, USA). Then, 10,000 cells were measured using consort 30. This was followed by an analysis of the total hemocyte counts (THC), characterization of hemocyte populations, hemocyte viability, and phagocytosis rate.
Total hemocyte count and characterization of hemocyte populations
The prescribed volume (300 µl) of hemolymph was added in a tube containing 1 µl of SYBR Green I obtained by diluting the 10×commercial solution (Sigma, St. Louis, Missouri, USA) and 300 µl of 6% formalin with filtered seawater before flow cytometric analysis. Te total hemocyte count, hemocyte proportions, and other cytometric measurements (i.e., size and internal complexity) were determined on SYBR Green I-positive cells. Live and dead cells containing DNA were stained by SYBR Green [17].
Measurements were then conducted with a flow cytometer. The total hemocyte (Y) counts per 1 ml of hemolymph were calculated via the following formula [18]; Y=X/60×2×1,000.
Here, 'X' represents the hemocyte counts measured with a flow cytometer; '60' represents the flow rate of the flow cytometer per min; '2' represents the dilution coefficient; and '1,000' represents the constant by which µl can be converted to ml.
Hemocyte subtypes were discriminated based upon relative flow cytometric morphological parameters, Forward Scatter (FSC), and Side Scatter (SSC). FSC and SSC commonly measure particle size and internal complexity, respectively [16].
Hemocyte viability
Hemocyte viability was assayed with a Live/Dead® Viability/Cytotoxicity kit (Invitrogen, Carlsbad, California, USA) as described by Choi et al. [19]. This kit quickly discriminates live from dead cells by simultaneously staining with green fluorescent calcein-AM to indicate intracellular esterase activity and red fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. A 300-µl of hemolymph was added along with 1 µl of calcein-AM and 2 µl of 2 mM EthD-1 (ethidium homodimer-1), and diluted with DMSO, and the reaction was conducted in a dark room for 30 min. This was followed by measurements of hemocyte viability with a flow cytometer.
Phagocytosis rate
According to the total hemocyte count, hemolymph was added to the filtered sea water. Thus, the number of cells was set at approximately 1×106 cell/ml. A 3-µl of FITC-Zymosan (Sigma) was added at a volume of 300 µl hemolymph. The phagocytic activity was induced for 30 min in a dark room at 25℃ [20]. This was followed by addition of hemolymph fluid and an identical volume of 6% formalin. Finally, the phagocytosis rate was measured using a flow cytometer. As a negative control which is without zymosan, hemolymph was cultured for 30 min at 25℃ in a dark room. With the addition of 6% formalin, the phagocytosis rate was measured with a flow cytometer.
Statistical analyses
In order to evaluate the alterations in the activity of hosts immunecompetency depending on differences in parasitic infections in each parameter, a t-test was conducted using SPSS (Statistical Package for the Social Sciences), Version 18.0. In addition, we conducted an analysis of the simple correlations between the parameters via Spearman's correlation coefficient.
Total hemocyte count
In HAO, the hemocyte counts were 3.9×106 cell/ml at the first experimental session, 1.8×106 cell/ml at the second experimental session, and 2.7×106 cell/ml at the third experimental session. Additionally, in the oysters infected with ovarian parasites, the hemocyte counts were 7.9×106 cell/ml, 5.0×106 cell/ml and 2.0×106 cell/ml respectively (Fig. 2). Total hemocyte counts differed significantly between the oysters infected with ovarian parasites (mean value; 4.12×106±0.70×106 cell/ml, SD; 2.2×106) and the healthy appearing ones (mean value; 2.11×106±0.36×106 cell/ml, SD; 1.13×106) (P<0.05), and the degree of difference between the 2 groups was twice that value (Table 1).
Characterization of hemocyte populations
C. gigas hemocytes were classified on the basis of their size and granularity, and were divided into hyalinocytes (low SSC and low FSC) and granulocytes (high SSC and high FSC) (Fig. 3). In HAO, hyalinocytes were measured at 81.8±5.5%, and granulocytes were measured at 16.9±5.5%. In the M. chungmuensis-infected oysters, hyalinocytes were measured at 73.0±7.3% and granulocytes were measured at 25.2±7.2%. In the infected oysters, the granulocytes increased significantly as compared with HAO (P<0.05). In addition, hyalinocyte levels were reduced significantly (P<0.05).
Hemocyte viability
In HAO, the hemocyte viability was 78.7% in the first, 87.2% in the second, and 94.5% in the third experimental session. In M. chungmuensis-infected oysters, the hemocyte viability was 55.1% in the first experimental session, 85.0% in the second, and 93.6% in the third (Fig. 4). The hemocyte viability was 88.6±1.9% (SD; 6.0) in HAO and 86.3±4.0% (SD; 12.7) in the oysters infected with M. chungmuensis (Table 1). No significant differences in hemocyte viability were noted between the M. chungmuensis-infected and HAO (P>0.05) (Fig. 4). After an analysis of the simple correlation between the defensive factors contained in the hemocytes of both M. chungmuensis-infected and HAO (Table 2), the hemocyte viability was correlated negatively with the total hemocyte counts in the M.chungmuensis-infected oysters (P<0.05).
Phagocytosis rate
In HAO, the phagocytosis rate was 35.9% in the first experimental session, 39.8% in the second, and 34.5% in the third. In oysters infected with ovarian parasites, the phagocytosis rate was measured as 36.8% in the first experimental session, 41.9% in the second, and 50.5% in the third (Fig. 5). The phagocytosis rate was significantly higher in the oysters infected with M. chungmuensis relative to HAO (48.1±3.1% [SD; 8.9] vs 36.3±2.8% [SD; 9.9]) (P<0.05) (Table 1). After an analysis of the simple correlation between the defensive factors contained in the hemocytes of both M. chungmuensis-infected and HAO (Table 2), the phagocytosis rate was positively correlated with the total hemocyte counts (P<0.05). The phagocytosis rate increased at higher degrees of hemocyte viability during the control of pathogenic agents, and the hemocyte viability was reduced despite the elevation of total hemocyte counts as a defense mechanism (Table 2).
In the present study, in order to measure the activity of defensive factors including the phagocytic activity of C. gigas infected with M. chungmuensis, the immunological functions of hemocytes was evaluated using a flow cytometer. According to the findings of the current study, the total hemocyte counts were significantly higher in M. chungmuensis-infected oysters relative to HAO. In Mytilus galloprovincialis infected with Marteilia refrigens, the hemocyte counts have been shown to be significantly higher [21]. In Tapes philippinarum, also as the result of infection with Perkinsus and the reciprocal actions associated with exposure to detrimental algae, the circulating hemocyte counts are significantly affected [22]. As described herein, parasite infections have been reported to affect the total hemocyte counts.
In shellfish, the hemocytes have been variously classified according to their morphology, cytochemical properties, and immunological functions. According to most investigators, however, they can be classified into 2 types, granulocytes and hyalinocytes [23]. The granulocytes had an excellent phagocytic activity profile as compared with the hyalinocytes [24,25]. According to our study, the phagocytosis rate was significantly higher in M. chungmuensis-infected oysters, in which granulocytes increased significantly relative to HAO (P<0.05) (Fig. 5).
Additionally, as shown in Table 2, in M. chungmuensis-oysters, the total hemocyte counts and hemocyte viability were negatively correlated, with a Spearman's correlation coefficient of 0.9 (P<0.05). In contrast, the hemocyte viability in bivalves infected with Perkinsus marinus [26] and Vibrio tapetis [27] was significant. Our study demonstrated that the parasite infection could stimulate phagocytosis of hemocytes. The involvement of hemocytes in mollusk cellular immune responses mainly relies on their capacity to engulf and subsequently degrade foreign material through phagocytosis [13,28], as oyster immunity is the primary defense against pathogens [29].
It was also noted, however, that the phagocytosis rate increased at higher degrees of hemocyte viability during the control of pathogenic agents, and the hemocyte viability was reduced despite the elevation of total hemocyte counts as a defense mechanism (Table 2). These findings demonstrate that the hemocyte had a very powerful endocytotic capacity based on the phagocytotic activity, although it underwent apoptosis because it could not induce intracellular degradation against protozoan species. Despite their prompt responsiveness, they have been reported to mount no effective responses to protozoan species, such as Perkinsus and Bonamia [30,31].
According to our study, on flow cytometry, the total hemocyte counts were increased in oysters as the result of infection with M. chungmuensis. In particular, it was also demonstrated that the phagocytosis rate was raised as the result of increased proportions of granulocytes. This measure may not directly reflect an immune capacity, although most studies on bivalves use it as a biomarker of the defense system [26,32]. The measurement of alterations in the activity of defense parameters in oysters might prove to be a useful method for assessing the effects of M. chungmuensis on C. gigas. This might also be proven useful in evaluating the immunological status of hosts, thereby determining their health status.
This work was supported by National Fisheries Research and development Institute (NFRDI), Republic of Korea.
  • 1. Lee YC. The Study on the Development of Oyster Rack Culture System. Inha University. Maritime Affairs and Fisheries. Final Research Report. 1999. Seoul, Korea. p. 32-38.
  • 2. Park MS, Lyu HY, Lee TS. Investigation on the cause of bad natural seed collection of the pacific oyster, Crassostrea gigas: Relationships between the conditions of mother shell and the viability of the released eggs and larvae based on the pathological and embryological survey. J Korean Fish Soc 1999;32:62-67.
  • 3. Comps M, Park MS, Desports I. Fine structure of Marteilioides chungmuensis n.g., n. sp., parasite of oocyte of the oyster Crassostrea gigas. Aquaculture 1987;67:264-265.
  • 4. Itoh N, Oda T, Ogawa K, Wakabayashi H. Identification and development of a paramyxean ovarian parasite in the Pacific oyster Crassostrea gigas. Fish Pathol (Tokyo) 2002;37:23-28.
  • 5. Itoh N, Tun KL, Komiyama H, Ueki N, Ogawa K. An ovarian infection in the Iwagaki oyster, Crassostrea nippona, with the protozoan parasite Marteilioides chungmuensis. J Fish Dis 2004;27:311-314.
  • 6. Park MS, Kang CK, Choi DL, Jee BY. Appearance and pathogenicity of ovarian parasite Marteilioides chungmuensis in the farmed Pacific oysters, Crassostrea gigas, in Korea. J Shellfish Res 2003;22:475-479.
  • 7. Tun KL, Itoh N, Ueki N, Yoshinaga T, Ogawa K. Relationship between Marteilioides chungmuensis infection in the Pacific oyster, Crassostrea gigas. J Invertebr Pathol 2007;96:205-212.
  • 8. Itoh N, Oda T, Yoshinaga T, Ogawa K. DNA probes for detection of Marteilioides chungmuensis from the ovary of Pacific oyster Crassostrea gigas. Fish Pathol (Tokyo) 2003;38:163-169.
  • 9. Tun KL, Itoh N, Shimizu Y, Yamanoi H, Yoshinaga T, Ogawa K. Pathology of the protozoan parasite Marteilioides chungmuensis in the Pacific oyster, Crassostrea gigas. Int J Parasitol 2008;38:211-217.
  • 10. Jeong WG, Seo JH, Cho SM, Park CI. Influence of environmental factors on the prevalence of the ovarian parasite, Marteilioides chungmuensis, in Crassostrea gigas cultured in Pukman Bay, Tongyeong, Korea. Korean J Malacol 2005;21:33-40.
  • 11. Tun KL, Itoh N, Komiyama H, Ueki N, Yoshinaga T, Ogawa K. Comparison of Marteilioides chungmuensis infection in the Pacific oyster, Crassostrea gigas cultured in different conditions. Aquaculture 2006;253:91-97.
  • 12. Gliński Z, Jarosz J. Molluscan immune defenses. Arch Immunol Ther Exp (Warsz) 1997;45:149-155.
  • 13. Hooper C, Day R, Slocombe R, Handlinger J, Benkendorff K. Stress and immune responses in abalone: Limitations in current knowledge and investigative methods based on other models. Fish Shellfish Immunol 2007;22:363-379.
  • 14. Ashton-Alcox KA, Ford SE. Variability in molluscan hemocyte: A flow cytometric study. Tissue Cell 1998;30:195-204.
  • 15. Goedken M, DeGuise S. Flow cytometry as a tool to quantify the oyster phagocytosis, respiratory burst, and apoptosis. J Shellfish Res 2002;21:342.
  • 16. Donaghy L, Hong HK, Lambert C, Park HS, Shim WJ, Choi KS. First characterisation of the populations and immune-related activities of hemocytes from two edible gastropod species, the disk abalone, Haliotis discus discus and the spiny top shell, Turbo cornutus. Fish Shellfish Immunol 2010;28:87-97.
  • 17. Donaghy L, Kim BK, Hong HK, Park HS, Choi KS. Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis. Fish Shellfish Immunol 2009;27:296-301.
  • 18. Park KI, Park HS, Kim JM, Park YJ, Hong JS, Choi KS. Flow Cytometric Assessment of Immune Parameters of the Manila Clam (Ruditapes philippinarum). J Korean Fish Soc 2006;39:123-131.
  • 19. Choi DL, Lee NS, Kim MS, Seo JS, Park MA, Kim JW, Hwang JY. Flow cytometry analysis of softness syndrome effects on hemocytes of the tunicate Halocynthia roretzi. Aquaculture 2010;309:25-30.
  • 20. Rodrick GE. Effects of temperature, salinity and pesiticides on oyster hemocyte activity. Florida Water Resources J 2008;1:86-101.
  • 21. Carballal MJ, Villalba A, Lopez C. Seasonal variation and effects of age, food availability, size, gonadal development, and parasitism on the hemogram of Mytilus galloprovincialis. J Invertebr Pathol 1998;72:304-312.
  • 22. Hégaret H, Da Silva PM, Wikfors GH, Lambert C, De Bettignies T, Shumway SE, Soudant P. Hemocyte responses of Manila clams, Ruditapes philippinarum, with varying parasite, Perkinsus olseni, severity to toxic-algal exposures. Aquat Toxicol 2007;84:469-479.
  • 23. Hine PM. The inter-relationships of bivalve haemocytes. Fish Shellfish Immunol 1999;9:367-385.
  • 24. Bettencourt R, Dando P, Collins P, Costa V, Allam B, Serrão Santos R. Innate immunity in the deep sea hydrothermal vent mussel Bathymodiolus azoricus. Comp Biochem Physiol A Mol Integr Physiol 2009;152:278-289.
  • 25. Goedken M, DeGuise S. Flow cytometry as a tool to quantify oyster defence mechanisms. Fish Shellfish Immunol 2004;16:539-552.
  • 26. Soudant P, Paillard C, Choquet G, Lambert C, Reid HI, Marhic A, Donaghy L, Birkbeck TH. Impact of season and rearing site on the physiological and immunological parameters of the Manila clam Venerupis (=Tapes, =Ruditapes) philippinarum. Aquaculture 2004;229:401-418.
  • 27. Allam B, Paillard C, Auffret M, Ford SE. Effects of the pathogenic Vibrio tapetis on defence factors of susceptible and non-susceptible bivalve species. II. Cellular and biochemical changes following in vivo challenge. Fish Shellfish Immunol 2006;20:384-397.
  • 28. Tiscar PG, Mosca F. Defense mechanisms in farmed marine molluscs. Vet Res Commun 2004;28:57-62.
  • 29. Anderson RS, Beaven AE. Antibacterial activities of oyster (Crassostrea virginica) and mussel (Mytilus edulis and Geukensia demissa) plasma. Aquat Living Resources 2001;14:343-349.
  • 30. Bushek D, Ford SE, Alcox KA, Gustafson R, Allen SK Jr. Response of Crassostrea virginica to in vitro cultured Perkinsus marinus: Preliminary comparisons of thee inoculation methods. J Shellfish Res 1997;16:479-485.
  • 31. Sunila I, La Banca J. Apoptosis in the pathogenesis of infectious diseases of the Eastern oyster, Crassostrea virginica. Dis Aquat Org 2003;56:163-170.
  • 32. Gagnaire B, Soletchnik P, Faury N, Kerdudou N, Le Moine O, Renault T. Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field - Comparison of hatchery diploids and diploids from natural beds. Aquaculture 2007;264:449-456.
Fig. 1
Large (↑) and small (◃) nodules on the surface of the soft tissues of Crassostrea gigas caused by infection with Marteilioides chungmuensis.
kjp-49-229-g001.jpg
Fig. 2
Total hemocyte counts of healthy appearing oysters and M. chungmuensis-infected oysters evaluated via flow cytometry. *P<0.05.
kjp-49-229-g002.jpg
Fig. 3
Flow cytometer density plot of healthy appearing oysters (A) and M. chungmuensis-infected (B) C. gigas hemocytes allowing the distinction of subpopulations (hyalinocytes and granulocytes) according to their relative size (FS Lin) and granularity (SS Log).
kjp-49-229-g003.jpg
Fig. 4
Viability of healthy appearing oysters and M. chungmuensis-infected oysters evaluated via flow cytometry.
kjp-49-229-g004.jpg
Fig. 5
Phagocytosis measured as the mean fluorescence for healthy appearing oysters and M. chungmuensis-infected oysters. *P<0.05.
kjp-49-229-g005.jpg
Table 1.
Descriptive statistics of immunecompetency of the Pacific oyster, Crassostrea gigas
Table 1.
Contents Oysters Mean ± SE SD
Total hemocyte count Healthy appearing 2.1×106±0.4×106 1.1×106
Infected 4.1×106±0.7×106 2.2×106
Viability Healthy appearing 88.6±1.9 6.0
Infected 86.3±4.0 12.7
Phagocytosis Healthy appearing 36.3±2.8 8.9
Infected 48.1±3.1 9.9

SE, standard error; SD, standard deviation.

Table 2.
Spearman’s correlation analysis for immunecompetency of M. chungmuensis infection
Table 2.
Parameters Oysters Total hemocyte counts
 Viability
 Phagocytosis
Healthy appearing Infected Healthy appearing Infected Healthy appearing Infected
Total hemocyte counts Healthy appearing 1
Infected 0.51 1
Viability Healthy appearing 0.41 -0.70 1
Infected 0.10 -0.90* 0.90* 1
Phagocytosis Healthy appearing 0.81 0.10 0.40 0.20 1
Infected 0.41 -0.70 1.00** 0.9* 0.40 1

*(1-tailed), significant at P<0.05 level;

**(2-tailed), significant at P<0.01 level.

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Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study
Korean J Parasitol. 2011;49(3):229-234.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.229
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Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study
Korean J Parasitol. 2011;49(3):229-234.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.229
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Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study
Image Image Image Image Image
Fig. 1 Large (↑) and small (◃) nodules on the surface of the soft tissues of Crassostrea gigas caused by infection with Marteilioides chungmuensis.
Fig. 2 Total hemocyte counts of healthy appearing oysters and M. chungmuensis-infected oysters evaluated via flow cytometry. *P<0.05.
Fig. 3 Flow cytometer density plot of healthy appearing oysters (A) and M. chungmuensis-infected (B) C. gigas hemocytes allowing the distinction of subpopulations (hyalinocytes and granulocytes) according to their relative size (FS Lin) and granularity (SS Log).
Fig. 4 Viability of healthy appearing oysters and M. chungmuensis-infected oysters evaluated via flow cytometry.
Fig. 5 Phagocytosis measured as the mean fluorescence for healthy appearing oysters and M. chungmuensis-infected oysters. *P<0.05.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study
Contents Oysters Mean ± SE SD Total hemocyte count Healthy appearing 2.1×106±0.4×106 1.1×106 Infected 4.1×106±0.7×106 2.2×106 Viability Healthy appearing 88.6±1.9 6.0 Infected 86.3±4.0 12.7 Phagocytosis Healthy appearing 36.3±2.8 8.9 Infected 48.1±3.1 9.9 Parameters Oysters Total hemocyte counts
Viability
Phagocytosis
Healthy appearing Infected Healthy appearing Infected Healthy appearing Infected Total hemocyte counts Healthy appearing 1 Infected 0.51 1 Viability Healthy appearing 0.41 -0.70 1 Infected 0.10 -0.90* 0.90* 1 Phagocytosis Healthy appearing 0.81 0.10 0.40 0.20 1 Infected 0.41 -0.70 1.00** 0.9* 0.40 1
Table 1. Descriptive statistics of immunecompetency of the Pacific oyster, Crassostrea gigas

SE, standard error; SD, standard deviation.

Table 2. Spearman’s correlation analysis for immunecompetency of M. chungmuensis infection

(1-tailed), significant at P<0.05 level;

(2-tailed), significant at P<0.01 level.

Table 1.

Table 2.

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