Parasites, Hosts and Diseases

Skip to main navigation Skip to main content

OPEN ACCESS

Page Path

HOME
BROWSE ARTICLES

Author index detail contents
Sang Rock Cho	5 Articles
Localization of cytoskeletal proteins in Pneumocystis carinii by immuno-electron microscopy Jae-Ran Yu, Jae-Kyong Pyon, Min Seo, Byung-Suk Jung, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong Korean J Parasitol 2001;39(1):13-21. Published online March 31, 2001 DOI: https://doi.org/10.3347/kjp.2001.39.1.13 Abstract PDF Pneumocystis carinii causes serious pulmonary infection in immunosuppressed patients. This study was undertaken to observe the cytoskeletal proteins of P. carinii by immuno-electron microscopy. P. carinii infection was experimentally induced by immunosuppression of Sprague-Dawley rats for seven weeks, and their lungs were used for the observations of this study. The gold particles localized actin, tropomyosin, and tubulin. The actin was irregularly scattered in the cytoplasm of the trophic forms but was much more concentrated in the inner space of the cell wall of the cystic forms called the inner electron-lucent layer. No significant amount of tropomyosin was observed in either trophic forms or cystic forms. The tubulin was distributed along the peripheral cytoplasm and filopodia of both the trophic and cystic forms rather than in the inner side of the cytoplasm. Particularly, in the cystic forms, the amount of tubulin was increased and located mainly in the inner electron-lucent layer of the cell wall where the actin was concentrated as well. The results of this study showed that the cell wall of P. carinii cystic forms is a structure whose inner side is rich in actin and tubulin. The location of the actin and tubulin in P. carinii suggests that the main role of these proteins is an involvement in the protection of cystic forms from the outside environment by maintaining rigidity of the cystic forms. Citations Citations to this article as recorded by A Molecular Window into the Biology and Epidemiology of Pneumocystis spp Liang Ma, Ousmane H. Cissé, Joseph A. Kovacs Clinical Microbiology Reviews.2018;[Epub] CrossRef 8,713 View 78 Download Crossref Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong Korean J Parasitol 2000;38(3):151-158. Published online September 30, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.3.151 Abstract PDF Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA. Citations Citations to this article as recorded by Intraspecific variability in several isolates of Philasterides dicentrarchi (syn. Miamiensis avidus), a scuticociliate parasite of farmed turbot Belén Budiño, Jesús Lamas, María P. Pata, Juan A. Arranz, Manuel L. Sanmartín, José Leiro Veterinary Parasitology.2011; 175(3-4): 260. CrossRef Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee Chonnam Medical Journal.2008; 44(2): 82. CrossRef 7,974 View 75 Download Crossref Purification of a 68-kDa cysteine proteinase from crude extract of Pneumocystis carinii Min-Ho Choi, Byung-Suk Chung, Young-Bae Chung, Jae-Ran Yu, Sang Rock Cho, Sung-Tae Hong Korean J Parasitol 2000;38(3):159-166. Published online September 30, 2000 DOI: https://doi.org/10.3347/kjp.2000.38.3.159 Abstract PDF The present study intended to verify activities of cysteine proteinase of Pneumocystis carinii from rats and to purify the enzyme. In order to exclude the contamination of host-derived enzymes, concentrates of P. carinii was primarily treated with a mixture of proteinase inhibitors before lysis of P. carinii. A 68-kDa cysteine proteinase was finally purified from the crude extract of P. carinii by 4 sequential chromatographic methods. The enzyme showed an optimal activity at pH 5.5 in 0.1 M sodium acetate, and its activity was specifically inhibited by L-trans-epoxysuccinylleucylamido (4-guanidino) butane (E-64) and iodoacetic acid, suggesting that the enzyme is a cysteine proteinase. The 68-kDa proteinase weakly digested macromolecules such as collagen, hemoglobin and fibronectin. The present study demonstrated the activity of cysteine proteinase at the 68-kDa band of P. carinii, and purified and characterized the molecule. Citations Citations to this article as recorded by Comparative Genomics Suggests that the Fungal Pathogen Pneumocystis Is an Obligate Parasite Scavenging Amino Acids from Its Host's Lungs Philippe M. Hauser, Frédéric X. Burdet, Ousmane H. Cissé, Laurent Keller, Patrick Taffé, Dominique Sanglard, Marco Pagni, Jason E. Stajich PLoS ONE.2010; 5(12): e15152. CrossRef Characterization of a Novel ADAM Protease Expressed byPneumocystis carinii Cassie C. Kennedy, Theodore J. Kottom, Andrew H. Limper Infection and Immunity.2009; 77(8): 3328. CrossRef 8,742 View 75 Download Crossref Karyotypes of Pneumocystis carinii derived from several mammals Sang Rock Cho, Yun-Gyu Park, Hyung Nam Moon, Soon-Hyung Lee, Sung-Tae Hong Korean J Parasitol 1999;37(4):271-275. Published online December 31, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.4.271 Abstract PDF Pneumocystis carinii is the most important opportunistic pathogen of humans in the world. Pneumocystis carinii is experimentally detected in the lungs of rats, mice, rabbits, and monkeys, however, the organisms from different mammals are identical in microscopic morphology. The present study tried to find out more mammalian hosts of P. carinii and also to differentiate the organisms from different mammals by karyotyping. Rats, mice, hamsters, rabbits, cats, and dogs were successfully infected by P. carinii, but guinea pigs and pigs were not. Karyotype of P. carinii from rabbits showed similar size range of chromosomes with that of the prototype, but in different pattern. The patterns from cats and dogs were also different from that of rats. The present study confirms that cats and dogs are infected by P. carinii and at least total three karyotype strains of P. carinii are proven in Korea. Citations Citations to this article as recorded by Axenic Long-Term Cultivation of Pneumocystis jirovecii Diana Riebold, Marie Mahnkopf, Kristina Wicht, Cristina Zubiria-Barrera, Jan Heise, Marcus Frank, Daniel Misch, Torsten Bauer, Hartmut Stocker, Hortense Slevogt Journal of Fungi.2023; 9(9): 903. CrossRef Respiratory Diseases in Guinea Pigs, Chinchillas and Degus María Ardiaca García, Andrés Montesinos Barceló, Cristina Bonvehí Nadeu, Vladimír Jekl Veterinary Clinics of North America: Exotic Animal Practice.2021; 24(2): 419. CrossRef Molecular detection ofPneumocystisin the lungs of cats Patrizia Danesi, Michela Corrò, Christian Falcaro, Antonio Carminato, Tommaso Furlanello, Monia Cocchi, Mark B Krockenberger, Wieland Meyer, Gioia Capelli, Richard Malik Medical Mycology.2018;[Epub] CrossRef Pneumocystis: from a doubtful unique entity to a group of highly diversified fungal species Magali Chabé, Cécile-Marie Aliouat-Denis, Laurence Delhaes, El Moukhtar Aliouat, Eric Viscogliosi, Eduardo Dei-Cas FEMS Yeast Research.2011; 11(1): 2. CrossRef Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee Chonnam Medical Journal.2008; 44(2): 82. CrossRef Pneumocystis species, co-evolution and pathogenic power Cécile-Marie Aliouat-Denis, Magali Chabé, Christine Demanche, El Moukhtar Aliouat, Eric Viscogliosi, Jacques Guillot, Laurence Delhaes, Eduardo Dei-Cas Infection, Genetics and Evolution.2008; 8(5): 708. CrossRef Pneumocystis James R. Stringer International Journal of Medical Microbiology.2002; 292(5-6): 391. CrossRef Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong The Korean Journal of Parasitology.2000; 38(3): 151. CrossRef 9,228 View 69 Download Crossref Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis Sung-Tae Hong, Mejeong Lee, Nak-Jin Sung, Sang Rock Cho, Jong-Yil Chai, Soon-Hyung Lee Korean J Parasitol 1999;37(4):243-248. Published online December 31, 1999 DOI: https://doi.org/10.3347/kjp.1999.37.4.243 Abstract PDF The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and IgG2 antibodies were produced but not specific, IgG3 antibody was least produced, and IgG4 antibody was prominent and specific. The serum IgG antibody reaction to any of 43-50, 34-37, 26-28, and 8 kDa bands was found in 65.5% of 168 egg positive cases while IgG4 antibody reaction was found in 22.0% of them. The positive rates of IgG and IgG4 antibodies were directly correlated with the intensity of infection. All of the sera from heavily infected cases over EPG 5,000 showed positive reaction for specific IgG and IgG4 antibodies. The specific serum IgG4 antibody disappeared within 6 months after treatment. The bands of 35 kDa and 67 kDa cross-reacted with IgG antibodies but not with IgG4 antibodies in sera of other trematode infections. The present findings suggest that serum IgG4 antibody reaction to 8 kDa band is specific but not sensitive. Any method to increase its sensitivity is required for improved serodiagnosis. Citations Citations to this article as recorded by Initial misdiagnosis to a revised diagnosis of pulmonary paragonimiasis with elevated serum immunoglobulin G4 concentrations: a case report Pengyan Yin, Miaohui Duan, Lingyao Deng, Tianli Li, Xuan Huang, Raohong Fang, Hailong Li, Binliang Bai Journal of International Medical Research.2025;[Epub] CrossRef Imported Familial Pulmonary and Cerebral Paragonimiasis in Korea: Cases and Literature Review from a Clinical Grand Round Conference Ji Young Lee, Jee Yeon Baek, Haesung Yoon, Mi-Jung Lee, Hoon-Chul Kang, Se Hee Kim, Joon Soo Lee, Ji-Man Kang, Jong Gyun Ahn Infection & Chemotherapy.2025; 57(4): 462. CrossRef An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection Lakkhana Sadaow, Rutchanee Rodpai, Penchom Janwan, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan Tropical Medicine and Infectious Disease.2022; 7(10): 308. CrossRef High prevalence of opisthorchiasis in rural populations from Khammouane Province, central Lao PDR: serological screening using total IgG- and IgG4-based ELISA Weeraya Phupiewkham, Rutchanee Rodpai, Somchith Inthavongsack, Sakhone Laymanivong, Tongjit Thanchomnang, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Oranuch Sanpool, Penchom Janwan, Pewpan M Intapan, Wanchai Maleewong Transactions of The Royal Society of Tropical Medicine and Hygiene.2021; 115(12): 1403. CrossRef Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis Weeraya Phupiewkham, Lakkhana Sadaow, Oranuch Sanpool, Rutchanee Rodpai, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan Parasitology Research.2021; 120(8): 2839. CrossRef Development of an Immunochromatographic Point-of-Care Test for Serodiagnosis of Opisthorchiasis and Clonorchiasis Lakkhana Sadaow, Oranuch Sanpool, Rutchanee Rodpai, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, Wanchai Maleewong, Pewpan M. Intapan The American Journal of Tropical Medicine and Hygiene.2019; 101(5): 1156. CrossRef The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection Jin Xu, Lexun Wang, Chuanhuan Deng, Xuchu Hu, Ran Li, Tingjin Chen, Dan Ning, Yingsong Wu, Yan Huang, Xuerong Li, Zhongdao Wu, Xinbing Yu, Yonglong Li Parasitology Research.2014; 113(1): 149. CrossRef 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory–secretory antigen Yanwen Li, Yan Huang, Xuchu Hu, Xiaoquan Liu, Changling Ma, Junhong Zhao, Zhongdao Wu, Jin Xu, Xinbing Yu Parasitology Research.2012; 111(2): 673. CrossRef Evaluation and Characterization of Fasciola hepatica Tegument Protein Extract for Serodiagnosis of Human Fascioliasis Adelaida Morales, Ana M. Espino Clinical and Vaccine Immunology.2012; 19(11): 1870. CrossRef Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334. CrossRef The identification of a Clonorchis sinensis gene encoding an antigenic egg protein Mejeong Lee, Young-Bae Chung, Suk-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong Parasitology Research.2005; 95(3): 224. CrossRef Evaluation of Immunoglobulin G Subclass Antibodies against RecombinantFasciola giganticaCathepsin L1 in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Fasciolosis Chaisiri Wongkham, Chairat Tantrawatpan, Pewpan M. Intapan, Wanchai Maleewong, Sopit Wongkham, Kunio Nakashima Clinical and Vaccine Immunology.2005; 12(10): 1152. CrossRef Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong The Korean Journal of Parasitology.2003; 41(1): 35. CrossRef Immunology and molecular biology of Opisthorchis viverrini infection S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha Acta Tropica.2003; 88(3): 195. CrossRef Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda) Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong The Korean Journal of Parasitology.2003; 41(3): 155. CrossRef Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis Young-Bae Chung, Mejeong Lee, Hyun-Jong Yang, Byung-Suk Chung, Shun-Yu Lee, Min-Ho Choi, Sung-Tae Hong The Korean Journal of Parasitology.2002; 40(2): 83. CrossRef Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang The Korean Journal of Parasitology.2001; 39(4): 307. CrossRef Cystatin Capture Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Clonorchiasis and Profile of Captured Antigenic Protein ofClonorchis sinensis Tae Yun Kim, Shin-Yong Kang, Sun Hyo Park, Kom Sukontason, Kabkaew Sukontason, Sung-Jong Hong Clinical Diagnostic Laboratory Immunology.2001; 8(6): 1076. CrossRef Partial characterization of a 17 kDa protein of Clonorchis sinensis Young-Bae Chung, Byung-Suk Chung, Min-Ho Choi, Jong-Yil Chai, Sung-Tae Hong The Korean Journal of Parasitology.2000; 38(2): 95. CrossRef 9,447 View 68 Download Crossref