Parasites, Hosts and Diseases

Skip to main navigation Skip to main content

OPEN ACCESS

Page Path

HOME
BROWSE ARTICLES

Author index detail contents
Sera Kim	3 Articles
ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam Korean J Parasitol 2003;41(4):203-207. Published online December 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.4.203 Abstract PDF An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used as template to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1). Each amplified DNA fragment was inserted into pQE30 plasmid to induce the expression of His-tagged protein in Escherichia coli (M15 strain) by IPTG. His-tagged proteins were purified by Ni-NTA metal-affinity chromatography and used as antigens for ELISA with patient sera that were confirmed previously by blood smear examinations. When applied to patient sera, 122 (80.3%) out of 152 vivax malaria cases reacted to at least one antigen, while no reactions were observed with 128 uninfected serum samples. We applied this ELISA to the screening of 3,262 civilian residents in endemic regions near the DMZ, which resulted in 236 positively detected (7.2%) cases. This method can be applied to serological diagnosis and mass screening in endemic regions, or can be used as a safety test for transfusion blood in endemic areas. Citations Citations to this article as recorded by Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review Lejla Kartal, Ivo Mueller, Rhea J. Longley Pathogens.2023; 12(6): 791. CrossRef Antibodies Against the Plasmodium vivax Apical Membrane Antigen 1 From the Belem Strain Share Common Epitopes Among Other Worldwide Variants Ana Caroline Barbosa França, Kátia Sanches Françoso, Rodolfo Ferreira Marques, Gustavo H. G. Trossini, Renan A. Gomes, Marinete M. Póvoa, Maristela G. Cunha, Eduardo L. V. Silveira, Irene S. Soares Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef Analyses of the expression, immunohistochemical properties and serodiagnostic potential of Schistosoma japonicum peroxiredoxin-4 Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masashi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-ichiro Kawazu Parasites & Vectors.2020;[Epub] CrossRef Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species Kharleezelle J. Moendeg, Jose Ma. M. Angeles, Yasuyuki Goto, Lydia R. Leonardo, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu Parasitology Research.2015; 114(3): 1225. CrossRef Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim The Korean Journal of Parasitology.2014; 52(2): 143. CrossRef Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis Julia C Cutts, Rosanna Powell, Paul A Agius, James G Beeson, Julie A Simpson, Freya J I Fowkes BMC Medicine.2014;[Epub] CrossRef A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran Afsaneh Motevalli Haghi, Mohammad Reza Khoramizade, Mehdi Nateghpour, Mehdi Mohebali, Gholam Hossein Edrissian, Mohammad Reza Eshraghian, Zargham Sepehrizadeh The Korean Journal of Parasitology.2012; 50(1): 15. CrossRef Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale George J. Dawson, Suresh M. Desai, Larry Birkenmeyer, A. Scott Muerhoff The American Journal of Tropical Medicine and Hygiene.2010; 82(6): 996. CrossRef Detection ofPlasmodium falciparum,P. vivax,P. ovale, andP. malariaeMerozoite Surface Protein 1-p19 Antibodies in Human Malaria Patients and Experimentally Infected Nonhuman Primates A. Scott Muerhoff, Larry G. Birkenmeyer, Ruthie Coffey, Bruce J. Dille, John W. Barnwell, William E. Collins, Joann S. Sullivan, George J. Dawson, Suresh M. Desai Clinical and Vaccine Immunology.2010; 17(10): 1631. CrossRef A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies Cecile Doderer, Aurelie Heschung, Phillippe Guntz, Jean-Pierre Cazenave, Yves Hansmann, Alexandre Senegas, Alexander W Pfaff, Tamer Abdelrahman, Ermanno Candolfi Malaria Journal.2007;[Epub] CrossRef 8,220 View 82 Download Crossref ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam Korean J Parasitol 2003;41(3):175-177. Published online September 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.3.175 Abstract PDF An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals. Citations Citations to this article as recorded by Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Roloff, Fábio Pereira Leivas Leite Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 148. CrossRef Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum Gizele Lima de Sá, Diene de Borba Pacheco, Leonardo Garcia Monte, Francine Alves Sinnott, Marina Amaral Xavier, Caroline Rizzi, Sibele Borsuk, Maria Elisabeth Aires Berne, Renato Andreotti, Cláudia Pinho Hartleben Current Microbiology.2014; 68(4): 472. CrossRef Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite Pathogens and Global Health.2013; 107(3): 116. CrossRef Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle Sibele Borsuk, Renato Andreotti, Fábio Pereira Leivas Leite, Luciano da Silva Pinto, Simone Simionatto, Claudia Pinho Hartleben, Marcelo Goetze, Leandra Marla Oshiro, Maria de Fátima Cepa Matos, Maria Elisabeth Aires Berne Veterinary Parasitology.2011; 177(1-2): 33. CrossRef Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA Jing Liu, Jinshu Yu, Ming Wang, Qun Liu, Wei Zhang, Chong Deng, Jun Ding Veterinary Parasitology.2007; 143(3-4): 358. CrossRef Epidemiology and Control of Neosporosis andNeospora caninum J. P. Dubey, G. Schares, L. M. Ortega-Mora Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef Diagnosis of bovine neosporosis: Recent advances and perspectives Luis Ortega-Mora, Aurora Fernández-García, Mercedes Gómez-Bautista Acta Parasitologica.2006; 51(1): 1. CrossRef Diagnosis of bovine neosporosis J.P. Dubey, G. Schares Veterinary Parasitology.2006; 140(1-2): 1. CrossRef Comparison of proteome and antigenic proteome between two Neospora caninum isolates Yong-Seung Shin, Gee-Wook Shin, Young-Rim Kim, Eun-Young Lee, Hyang-Hee Yang, K.J. Palaksha, Hee-Jeong Youn, Jae-Hoon Kim, Dae-Yong Kim, A.E. Marsh, J. Lakritz, Tae-Sung Jung Veterinary Parasitology.2005; 134(1-2): 41. CrossRef HPLC purification of recombinant NcGRA6 antigen improves enzyme-linked immunosorbent assay for serodiagnosis of bovine neosporosis M.C. Jenkins, R. Fetterer, G. Schares, C. Björkman, W. Wapenaar, M. McAllister, J.P. Dubey Veterinary Parasitology.2005; 131(3-4): 227. CrossRef Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics Yong‐seung Shin, Eung‐goo Lee, Gee‐wook Shin, Young‐rim Kim, Eun‐young Lee, Jae‐hoon Kim, Hwan Jang, Laurel J. Gershwin, Dae‐yong Kim, Yong‐hwan Kim, Gon‐sup Kim, Myung‐deuk Suh, Tae‐sung Jung PROTEOMICS.2004; 4(11): 3600. CrossRef Development of competitive ELISA for neosporosis by employing immunoproteomics Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung Clinical Proteomics.2004;[Epub] CrossRef 8,114 View 77 Download Crossref Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients Hye-Jin Ahn, Sera Kim, Ho-Woo Nam Korean J Parasitol 2003;41(2):89-96. Published online June 20, 2003 DOI: https://doi.org/10.3347/kjp.2003.41.2.89 Abstract PDF Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody. Citations Citations to this article as recorded by Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay Protein Expression and Purification.2009; 65(1): 8. CrossRef Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis Houshuang Zhang, Eung-goo Lee, Min Liao, Muller K.A. Compaore, Guohong Zhang, Osamu Kawase, Kozo Fujisaki, Chihiro Sugimoto, Yoshifumi Nishikawa, Xuenan Xuan Molecular and Biochemical Parasitology.2007; 153(2): 141. CrossRef Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii-specific IgG using Faradaic impedance spectroscopy, CV and QCM Yanjun Ding, Hua Wang, Guoli Shen, Ruqin Yu Analytical and Bioanalytical Chemistry.2005; 382(7): 1491. CrossRef Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef The P Domain of the P0 Protein ofPlasmodium falciparumProtects against Challenge with Malaria Parasites K. Rajeshwari, Kalpesh Patel, Savithri Nambeesan, Monika Mehta, Alfica Sehgal, Tirtha Chakraborty, Shobhona Sharma Infection and Immunity.2004; 72(9): 5515. CrossRef 8,395 View 66 Download Crossref