The glucose uptake rate by plerocercoid of Diphyllobothrium sp. was a mean value of 5.35+/-0.80 micro-mole/hr/g of wet wt, and total CO(2) production rates by the plerocercoid larva averaged 7.54+/-0.73 micro-mole/hr/g of wet wt. The relative specific activity into respiratory CO(2) showed a mean value of 7.30 +/-0.90 per cent. The rate of CO(2) production derived from medium C(14)-glucose was a mean of 0.58+/-0.13 micro-mole/hr/g of wet wt. Therefore, the average value of 1.92+/-0.38 per cent of glucose utilized by the larvae from the medium C(14)-glucose was oxidized to respiratory CO(2). The tissue concentration of glycogen in plerocercoid larva was a mean of 46.28 +/-2.23 mg/g or 4.63+/-0.22 per cent/g of wet wt., and the turnover rate of glycogen pool was a mean of 0.049 +/- 0.012 %/hr or 0.010 +/- 0.003 mg/hr/g of wet wt. The average value of 2.76+/-1.00 per cent of glucose utilized by the larvae from the medium C(14)-glucose was incorporated to the glycogen.
These data accounts for that only 5 per cent of the utilized glucose by the plerocercoid larvae participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen.
Citations
Citations to this article as recorded by
Study on the (14)C-glucose metabolism by Clonorchis sinensis Il Kwon Kang, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1969; 7(3): 143. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Metabolism of C(14)-glucose by Moniezia expansa and Diphyllobothrium mansoni Han Jong Rim, Soo Hyun Seong, Chung Jai Park, Sang Don Rhee, Byong Jong Oh, Hyu Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 112. CrossRef
Metabolism of C(14)-glucose by Paramphistomum cervi Byong Seol Seo, Han Jong Rim, Sang Il Lee, Dae Kwan Park, Sang Chan Moon The Korean Journal of Parasitology.1965; 3(1): 5. CrossRef
Metabolism of C14-lactate by Fasciola hepatica and Eurytrema pancreaticum Sang Il Lee The Korean Journal of Parasitology.1965; 3(1): 10. CrossRef
Metabolism of C(14)-glucose by Ascaridia galli Han Jong Rim, Kwang Soo Kim, Soo Hyun Seong, Sang Don Rhee, Byong Jong On, Hyun Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 107. CrossRef
Metabolism of C(14)-acetate by cestodes Han Jong Rim, Chung Jai Park, Yong Ok Min, Byong Jong On, Hyun Kyo Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 122. CrossRef
The trematode Paramphistomum cervi empolyed in this experiment was obtained from the reticulum of cattle slaughtered at the local abbatoir. The worms were selected and washed several times in normal sterilized saline solution. Each about ten of intact worms were incubated in 50 cc volume of special incubation flasks with incubation mixture consisting of 50 cc of Krebs-Ringer phosohate buffer (pH 7.4) to which were added universally labeled C(14)-glucose and non-radioactive carrier glucose concentration of 200 mg per cent. The worms were allowed to incubate for 3 hours in the incubator at 38 C. After incubation period, respiratory CO(2) samples from central wall of incubation flask were analysed for total CO(2) production rate and their specific activity of respiratory CO(2). Glycogen samples isolated from worms were analysed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. The glucose uptake rate was determined by analysing the difference of the glucose concentration in a medium before and after incubation period. Radioactivities of these series of experiments were counted by an endwindow Geiger-Muller counter as an infinitely thin samples. The quantitative analysis of C(14)-glucose utilized by Paramphistomum cervi was summerized as the following. The glucose uptake rate by Paramphistomum was a mean value of 2.32+/-0.27 micro-mole/hr/g of wet wt. and total CO(2) production rate by the worms averaged 10.85+/-0.41 micro-mole/hr/g of wet wt. The relative specific activities of respiratory CO(2) averaged 49.72+/-13.20 per cent. Thus, a mean of 49.72 per cent of total CO(2) production rate was originated from the glucose in the medium, therefore the rate of CO(2) production derived from medium glucose was mean of 5.24+/-2.16 micro-mole/hr/g of wet wt. Thus, the average value of 37.46+/-5.28 per cent of glucose utilized by the worms from the medium glucose was oxidized to respiratory CO(2). The tissue concentration of Paraphismum was a mean of 41.56+/-5.82 micro-mole/hr/g of wet wt or 4.16+/-0.72 per cent/g , and the turnover rate of glycogen pool yielded with a mean of 0.12+/-0.014 percent/hr or 0.06+/-0.04 mg/hr/g of wet wt. Therefore, a mean value of 16.75+/-4.84 per cent of glucose was incorporated to the glycogen. These data account for that at least 54.21 per cent of the utilized glucose by the worms participated in furnishing the oxidation into respiratory CO(2) and the synthetic process into glycogen.
According to the above data of the experiment, it is suggested in the metabolic process of glucose by the Paramphistomum that the synthetic process into the glycogen is less active than the oxidative process into the resppiratory CO(2).
Citations
Citations to this article as recorded by
Study on the (14)C-glucose metabolism by Clonorchis sinensis Il Kwon Kang, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1969; 7(3): 143. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Metabolism of C14-lactate by Fasciola hepatica and Eurytrema pancreaticum Sang Il Lee The Korean Journal of Parasitology.1965; 3(1): 10. CrossRef
Metabolism of C(14)-glucose by Ascaridia galli Han Jong Rim, Kwang Soo Kim, Soo Hyun Seong, Sang Don Rhee, Byong Jong On, Hyun Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 107. CrossRef
Metabolism of C(14)-glucose by Moniezia expansa and Diphyllobothrium mansoni Han Jong Rim, Soo Hyun Seong, Chung Jai Park, Sang Don Rhee, Byong Jong Oh, Hyu Kyo Lee The Korean Journal of Parasitology.1965; 3(3): 112. CrossRef
Metabolism of C(14)-acetate by cestodes Han Jong Rim, Chung Jai Park, Yong Ok Min, Byong Jong On, Hyun Kyo Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 122. CrossRef
Metabolism of C(14)-acetate by some trematodes Byong Seol Seo, Han Jong Rim, Yong Ok Min, Sang Don Rhee, Tong Hoon Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 117. CrossRef
The adult trematode, Fasciola hepatica and Eurytrema pancreaticum, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. The worms were selected and washed several times in normal sterilized saline solution. Each ten of intact F. hepatica and about thirty to fifty of E. pancreaticum were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 50 cc of Krebs-Ringer phosohate buffer (pH 7.4). The incubation medium was added C(14)-lactate and non-radioactive carrier Na-lactate so as to contain lactate concentration of 32 mg per cent. The worms were allowed to incubate for 3 hours in the Dubnoff metabolic shaking incubator at 38 C. After incubation period, respiratory CO(2) samples from central wall of incubation flask were analysed for total CO(2) production rate and their specific activity of respiratory CO(2). The lactate uptake rate was determined by analyzing the the difference between lactate concentration in a medium before and after the incubation period, and the pyruvate appearance rate was dertermined by analyzing the pyruvate concentration in a medium after incubation. The glycogen samples isolated from worms were analyzed for the tissue concentration and their radioactivities in order to determine the turnover rate of glycogen pool. Radioactivities of these serise of experiment were counted by an endwindow Geiger-Muller counter as an infinitely thin samples. The quantative analysis of C(14)-lactate utilized by F. hepatica and E. pancreaticum were summerized and compared as following. In F. hepatica the lactate uptake rate was a mean value of 1.04+/-0.15 micromole/hr/g of wet wt. and pyruvate apperance rate was a mean value of 0.132+/-0.005 micro-mole/hr/g of wet wt. The total CO(2) production rate by the flukes averaged 13.82+/-0.75 micro-mole/hr/g of wet wt. The relative specific activities of respiratory CO(2) showed a mean value of 9.93+/-0.62 per cent. The rate of CO(2) production derived from medium C(14)-lactate was a mean of 1.38+/-0.13 micro-mole/hr/g of wet wt. Therefore the averge value of 55.27+/-5.78 per cent (R.L.D. CO(2)) and 15.35+/-1.90 per cent (R.L.D. pyr) of lactate was oxidized into respiratory CO(2) and pyruvate respectively. On the other hand, in E.
pancreaticum the lactate uptake rate was a mean value of 0.61+/-0.18 micromole/hr/g of wet wt, and pyruvate appearance rate was a mean of 0.023+/-0.001 micromole/hr/g of wet wt. The total CO(2) production rate by the E.
pancreaticum averaged 4.29+/-0.85 micromole/hr/g of wet wt.
The relative specific activity of respiratory CO(2) (R.S.A CO2) showed a mean value of 9.20+/-0.34 per cent. Thus, a mean value of 9.20 per cent of total CO(2) production rates was originated from C14-lactate in a medium, therefore the rate of CO(2) production derived from medium C(14)-lactate was a mean value of 0.40+/-0.10 micromole/hr/g of wet wt.
The average value of 23.93+/-7.11 per cent(R.L.D. CO(2)) and 3.86+/-0.45 per cent(R.L.D. pyr) of lactate was oxidized into respiratory CO(2) and pyruvate respectively. The tissue concentration of glycogen in F. hepatica was a mean of 2.63 per cent/g of wet wt, while in E. pancreaticum was a mean of 4.06 per cent/g of wet wt. The turnover rate of glycogen pool in F. hepatica yielded a value of 0.073+/-0.008 micromole/hr/g of wet wt whereas in E. pancreaticum yielded only a mean of 0.006+/-0.002 mg/hr/g of wet wt. Therefore, the half time of glycogen turnover, which is the time interval required to replace the half of glycogen pool with medium C(14)-lactate, gave value of a mean of 10.73+_0.76 days in F. hepatica. However, incorporation of C(14)-lactate into glycogen was negligible in the E. pancreaticum. Theses data impressed that the carbohydrate such as lactate may play a role of major part of their oxidative metabolism in F. hepatica, whereas minor part of lactate participates in the oxidative metabolism in E. pancreaticum.
Citations
Citations to this article as recorded by
Autoradiographic study on the uptake of (3)H-6-thymidine by Fasciola hepatica Joong Ho Kim The Korean Journal of Parasitology.1973; 11(1): 13. CrossRef
Study on the (14)C-glucose metabolism by Clonorchis sinensis Il Kwon Kang, Soon Hyung Lee, Byong Seol Seo The Korean Journal of Parasitology.1969; 7(3): 143. CrossRef
Autoradiographic study on Eurytrema pancreaticum Seung Chull Park, Bon Ryong Koo, Byong Seol Seo The Korean Journal of Parasitology.1967; 5(3): 135. CrossRef
Metabolism of C(14)-glycine by Clonorchis sinensis Soo Hyun Seong, Byong Seol Seo The Korean Journal of Parasitology.1966; 4(2): 14. CrossRef
Metabolism of C(14)-acetate by some trematodes Byong Seol Seo, Han Jong Rim, Yong Ok Min, Sang Don Rhee, Tong Hoon Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 117. CrossRef
Metabolism of C(14)-acetate by cestodes Han Jong Rim, Chung Jai Park, Yong Ok Min, Byong Jong On, Hyun Kyo Lee, Myong Soon Yun The Korean Journal of Parasitology.1965; 3(3): 122. CrossRef
Authors carried out experimental study on chemotherapy with oral administration of Dithiazanine iodide (D.I.) and Bithionol sulfoxide(B.S.) in rabbit clonorchiasis. And the following result was obtained. In change of the E.P.G (eggs per gram feces) by D.I. administration, it was rather increased in early stage of the administration than prior to administration, and thereafter decreased gradually. In the change of the C.S. worm body by D.I. administration, there was not only prominent change of supporting tissue but also the change of reproductive organ was found. In considering the wormicidal effect of D.I. from detecting rate of survival worms, the effect was slight in group of 100 mg(80 mg/kg) per day dosage, but the effects were very excellent and almost complete by proper times of administration in groups of 200 mg (130 mg/kg) per day or higher dosage. But the side effect and intoxication sign of D.I. were appearent in groups of 200 mg or higher dosage. By B.S.
administration, E.P.G. was decreased gradually. In changes of the C.S. worm body by B.S. administration, prominent inhibitory chnnge was seen in egg formation ability. Slight wormicidal effect of B.S. was observed in groups of 140 mg (100 mg/kg) per day or higher dosage. Side effect and intoxication sign of B.S. were found little in groups of 140 mg or lesser dosage.
Citations
Citations to this article as recorded by
Epidemiological studies on Clonorchis sinensis infection along the Nam-river in Gyeongnam province, Korea Kyoung Hoon Bae, Yung Kyum Ahn, Chin Thack Soh, Hiroshi Tsutsumi The Korean Journal of Parasitology.1983; 21(2): 167. CrossRef
A study on the chemotherapy in clonorchiasis. Report 3. The patho-histological study on the liver of rabbit healed from clonorchiasis by chemotherapy Suck Yong Kang, Yong Soo Chun, In Kyun Loh, Eui Keun Ham The Korean Journal of Parasitology.1965; 3(3): 132. CrossRef
Metacercariae of a kind of Levinseniella species were found from a brackish water crabs, Macrophthalmus japonicus de Haan, in the brackish area of Hyungsan river of Kyungpook province. Habitats were the epigastric and progastric regions of Macrophthalmus japonicus. One hundred and seventy five crabs out of 178 examined (98.3 per cent) were infested with metacercariae of Levinseniella species. The infested number of metacercariae in each crabs was average 100 ea.
The excystation of metacercaria has not developed in vitro (both saline and tyrode's solution). Many eggs were observed in the metacercariae maintained for 24 or 48 hours in the water-bath. No differences were observed between semi-adult fluke from rat host and those maintained for 24 or 48 hours in vitro.
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Discovery ofMaritrema obstipum(Digenea: Microphallidae) from Migratory Birds in Korea Ok-Sik Chung, Woon-Mok Sohn, Jong-Yil Chai, Min Seo, Hye-Jung Lee The Korean Journal of Parasitology.2011; 49(4): 457. CrossRef
Discovery ofMaritrema jebuensisn. sp. (Digenea: Microphallidae) from the Asian Shore Crab,Hemigrapsus sanguineus, in Korea Ok-Sik Chung, Hye-Jung Lee, Woon-Mok Sohn, Seung-Ha Lee, Il-Yong Park, Sang-Ah Oh, Jong-Yil Chai, Min Seo The Korean Journal of Parasitology.2010; 48(4): 335. CrossRef
Gynaecotyla squatarolae (Digenea: Microphallidae) from rats experimentally infected with metacercariae from the shore crab, Macrophthalmus dilatatus Min Seo, Sang-Mee Guk, Seung-Ha Lee, Jong-Yil Chai The Korean Journal of Parasitology.2007; 45(3): 199. CrossRef
Studies on the metacercariae from fresh water fishes in the Kum-Ho River Jong Taek Lee The Korean Journal of Parasitology.1968; 6(3): 77. CrossRef
Studies on the larval trematodes from brackish water fishes 3. Observation on Pseudexorchis major (Hasegawa, 1935) Yamaguti, 1938 Dong Wik Choi, Whan Min Lee, Jong Taek Lee, Kyu Hyun Hwang The Korean Journal of Parasitology.1966; 4(2): 35. CrossRef
Polypeptide antigen as isolated from adult worms of Clonorchis sinensis by means of diluted HCl-extraction method. Preliminary intradermal examination with those polypeptide antigen(CPT) were performed on patients of clonorchiasis, paragonimiasis and metagonimiasis and the result of the intradermal test with CPT antigen were compared with those of Clonorchis protein fraction(CPF) and Veronal buffer saline extraction (VBS) antigen. The result of the examination were summerized as follows: When 0.05 ml (1 microgram) of CPT was injected intradermally, the positive intradermal reaction appeared in 42.7 per cent (37 of 82) on the patients of clonorchiasis. The sizes of the wheal and erythema with CPT antigen was smaller than that of CPF and VBS antigen. Intradermal reaction with CPT antigen was carried out for other parasitic diseases, such as paragonimiasis and metagonimiasis. The result indicated that CPT antigen may be showed no cross reactions. Clonorchiasis pateints who with negative reaction to CPT antigen, reacted negatively to CPF and VBS antigens.
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