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Volume 41(1); March 2003

Mini Review

Review of Neospora caninum and neosporosis in animals
J.P. Dubey
Korean J Parasitol 2003;41(1):1-16.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.1

Neospora caninum is a coccidian parasite of animals. It is a major pathogen for cattle and dogs and it occasionally causes clinical infections in horses, goats, sheep, and deer. Domestic dogs are the only known definitive hosts for N. caninum. It is one of the most efficiently transmitted parasite of cattle and up to 90% of cattle in some herds are infected. Transplacental transmission is considered the major route of transmission of N. caninum in cattle. Neospora caninum is a major cause of abortion in cattle in many countries. To elicit protective immunity against abortion in cows that already harbor a latent infection is a major problem. This paper reviews information on biology, diagnosis, epidemiology and control of neosporosis in animals.

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Original Articles

Tegumental ultrastructure of the juvenile and adult Himasthla alincia (Digenea: Echinostomatidae)
Eun-Taek Han, Kye-Young Han, Jong-Yil Chai
Korean J Parasitol 2003;41(1):17-25.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.17

The tegumental ultrastructure of juvenile and adult Himasthla alincia (Digenea: Echinostomatidae) was observed by scanning electron microscopy. One-, 5- (juveniles) and 20-day-old worms (adults) were harvested from chicks experimentally fed metacercariae from a bivalve, Mactra veneriformis. The juvenile worms were elongated and curved ventrally. The head crown bore 31 collar spines, arranged in a single row. The lip of the oral sucker had 12 paired, and 3 single type I sensory papillae, and the ventral sucker had about 25 type II sensory papillae. The anterolateral surface between the two suckers was densely packed with tegumental spines with 4-7 pointed tips. The adult worms were more elongated and filamentous, and had severe transverse folds over the whole body surface. On the head crown and two suckers, type I and II sensory papillae were more densely distributed than in the juvenile worms. Retractile brush-like spines, with 8-10 digits, were seen on the anterolateral surface, whereas claw-shaped spines, with 2-5 digits, were sparsely distributed posteriorly to the ventral sucker. The cirrus characteristically protruded out, and was armed with small spines distally. The surface ultrastructure of H. alincia was shown to be unique among echinostomes, especially in the digitation of its tegumental spines, the distribution of sensory papillae and by severe folds of the tegument.

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  • Echinostoma macrorchis in Lao PDR: Metacercariae in Cipangopaludina Snails and Adults from Experimentally Infected Animals
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  • FIRST RECORD AND DESCRIPTION OF METACERCARIAE OF CURTUTERIA ARGUINAE N. SP. (DIGENEA: ECHINOSTOMATIDAE), PARASITE OF COCKLES CERASTODERMA EDULE (MOLLUSCA: BIVALVIA) IN ARCACHON BAY, FRANCE
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Recovery, growth and development of Macroorchis spinulosus in albino rats
Ho-Chun Woo, Jong-Yil Chai, Sung-Jong Hong
Korean J Parasitol 2003;41(1):27-33.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.27

The developmental features, growth and organogenesis of Macroorchis spinulosus were observed in albino rats. Globular and thick walled metacercariae, possessed a stylet, Y-shaped excretory bladder and extracecal testes. In albino rats, M. spinulosus showed habitat shifting. The majority of M. spinulosus reside in the jejunum for the first four days post infection (p.i.) and migrate to the duodenum at the later stage of infection. M. spinulosus grew rapidly during the first four days and reached full maturity at 14 days p.i. and later reduced in size. The ovary was separated from the genital primodium at one day p.i. The seminal vesicle appeared on the third day and divided into two sacs on the fourth day p.i. and intrauterine eggs and sperm mass were produced on the fourth day. Organogenesis and enlargement of reproductive organs governed the growth of M. spinulosus. The similarity of related species of the genus Macroorchis to M. spinulosus was discussed in consideration to developmental features.

Citations

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  • Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences
    Eun Jeong Won, Deok-Gyu Kim, Jaeeun Cho, Bong-Kwang Jung, Min-Jae Kim, Yong Woon Yun, Jong-Yil Chai, Dong Wook Ryang
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  • Surface ultrastructure of the plagiorchid trematode Glossidium pedatum Looss, 1899 from bagrid fish in Egypt
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  • Developmental surface ultrastructure of Macroorchis spinulosus in albino rats
    Sung-Jong Hong, Ho-Chun Woo, Oh Sil Kwon
    The Korean Journal of Parasitology.2004; 42(4): 151.     CrossRef
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Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong
Korean J Parasitol 2003;41(1):35-39.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.35

Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

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Effects of gamma-irradiation on the infectivity and chromosome aberration of Clonorchis sinensis
Gab-Man Park, Tai-Soon Yong
Korean J Parasitol 2003;41(1):41-45.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.41

Effects of gamma irradiation on the worm survival and chromosomal aberration of Clonorchis sinensis were studied. The metacercariae irradiated with various amounts of gamma radiation (ranging from 5 Gy to 50 Gy) were fed to rats, and the effects were compared with those of non-irradiated controls. Recovery rates of adult worms in irradiated groups were reduced gradually as increasing of the irradiation doses. No worm was recovered from rats which were fed with 50 Gy irradiated metacercariae. The chromosome number was 2n = 56 in all worms from all experimental groups. However, the groups irradiated with 20 Gy, 25 Gy or 30 Gy showed variations in the chromosome number, depending on different cells in the same individual. Radiation doses used in this study did not appear to induce chromosome aberrations, however, irradiation with 30 Gy showed slightly reduced chromosome size.

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Phylogenetic relationship of ribosomal ITS2 and mitochondrial COI among diploid and triploid Paragonimus westermani isolates
Gab-Man Park, Kyung-Il Im, Tai-Soon Yong
Korean J Parasitol 2003;41(1):47-55.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.47

We compared patterns of intraspecific polymorphism of two markers with contrasting modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the lung fluke, diploid and triploid Paragonimus westermani from three geographical regions of Korea. The genetic distances between three populations of Korean diploid and triploid P. westermani showed no significant difference in the nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (mtCOI) and ribosomaal second internal transcribed spacer (ITS2) genes. A highly resolved strict-consensus tree was obtained that illustrated phylogenetically useful information of the ITS2 and mtCOI sequences from diploid and triploid P. westermani.

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A survey on head lice infestation in Korea (2001) and the therapeutic efficacy of oral trimethoprim/sulfamethoxazole adding to lindane shampoo
Seobo Sim, In-Yong Lee, Kyu-Jae Lee, Jang-Hoon Seo, Kyung-Il Im, Myeong Heon Shin, Tai-Soon Yong
Korean J Parasitol 2003;41(1):57-61.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.57

Total of 7,495 children including 3,908 boys and 3,587 girls from a kindergarten and 15 primary schools were examined for head lice infestation (HLI). The overall prevalence of HLI in this study was found to be 5.8%. Head lice were much more commonly detected in girls than in boys with prevalence of 11.2% and 0.9%, respectively. Sixty-nine children with HLI were treated with 1% lindane shampoo alone (group 1), and 45 children with HLI were treated with 1% lindane shampoo and oral trimethoprim/sulfamethoxazole (group 2), and follow-up visits were conducted 2 and 4 weeks later. The children who still had HLI 2 weeks after the primary treatment were treated again. At the 2-week follow-up visit, the treatment success rates of groups 1 and 2 were 76.8% and 86.7%, respectively, and at the 4-week follow-up visit, the rates were 91.3% and 97.8%, respectively. No statistically significant synergistic effect was observed for the combination of a 1% lindane shampoo and oral trimethoprim/sulfamethoxazole.

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Case Report

A case of gastric strongyloidiasis in a Korean patient
Jin Kim, Hyun-Soo Joo, Doo-Hong Kim, Ho Lim, Yu-Ho Kang, Myung-Soo Kim
Korean J Parasitol 2003;41(1):63-67.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.63

A 69-year-old Korean man was admitted to emergency room with complaints of abdominal pain, vomiting, and diarrhea. Laboratory tests revealed eosinophilia, anemia, hypoproteinemia, and hyponatremia. The gastric mucosa showed whitish mottled and slightly elevated lesions on the body angle of antrum. Microscopically, chronic gastritis with incomplete intestinal metaplasia was observed. Many adult worms, larvae, and eggs in cross sections were located in the crypts. Furthermore, the filariform larvae of Strongyloides stercoralis with a notched tail were detected through the culture.

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Brief Communications
A survey of Brugia malayi infection on the Heugsan Islands, Korea
Jong-Yil Chai, Soon-Hyung Lee, Sung-Yil Choi, Jong-Soo Lee, Tai-Soon Yong, Kyun-Jong Park, Kyeong-Ae Yang, Keon-Hoon Lee, Mi-Jeng Park, Hyung-Ran Park, Mi-Ja Kim, Han-Jong Rim
Korean J Parasitol 2003;41(1):69-73.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.69

Lymphatic filariasis due to Brugia malayi infection was endemic in several areas of South Korea. The infection was controlled, or disappeared, in most areas, with the exception of the remote southwestern islands of Jeonranam-do, including the Heugsan Islands. To discover its current situation, a small-scale survey was performed on the Heugsan Islands in September 2000. A total of 378 people, 151 male and 227 female, living in 8 villages (6 on Daeheugsan-do, 1 on Daejang-do, and 1 on Yeongsan-do) were subjected to a night blood survey for microfilaremia, and physical examination for elephantiasis on the extremities. There were 6 (1.6%) microfilaria positive cases, all in females aged 57-72 years, and from only two villages of the Daeheugsan-do area. There were 4 patients with lower leg elephantiasis, but they showed no microfilaremia. The results show that a low-grade endemicity of filariasis remains on the Daeheugsan-do.

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    Young Yil Bahk, Eun-Hee Shin, Shin-Hyeong Cho, Jung-Won Ju, Jong-Yil Chai, Tong-Soo Kim
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    Shin Hyeong Cho, Da Won Ma, Bo Ra Koo, Hee Eun Shin, Wook Kyo Lee, Byong Suk Jeong, Chaeshin Chu, Won Ja Lee, Hyeng Il Cheun
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    Heejeong Youn
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    Hyeng-Il Cheun, Yoon Kong, Shin-Hyeong Cho, Jong-Soo Lee, Jong-Yil Chai, Joo-Shil Lee, Jong-Koo Lee, Tong-Soo Kim
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    Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
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Egg positive rate of Enterobius vermicularis of primary school children in Geoje island
Bong Jin Kim, Bo Young Lee, Hyun Kee Chung, Young Sun Lee, Kun Hee Lee, Hae Jin Chung, Mee Sun Ock
Korean J Parasitol 2003;41(1):75-77.
Published online March 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.1.75

The status of pinworm (Enterobius vermicularis) egg positive rate of primary school children in Geoje island was investigated by using adhesive cellotape anal swap method, in September, 2002. Total egg positive rates of E. vermicularis were 9.8% (74/754) and those of male and female were 10.8% and 8.7%, respectively. Among three schools examined, Myeongsa primary school showed the highest egg positive rate (12.6%) followed by Yeoncho [9.8% (26/266)] and Geoje [9.1% (35/385)]. As for the age groups, the 2nd grade had the highest egg positive rate (15.3%), whereas the 5th grade showed the lowest egg positive rate (2.6%). The above result led us to confirm that the egg positive rates of E. vermicularis in primary school children in Geoje island were not significantly different from the those in the whole country including urban and rural areas, showing more than 10%.

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