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Volume 50(1); March 2012

Original Articles

Anti-Apoptotic Effects of SERPIN B3 and B4 via STAT6 Activation in Macrophages after Infection with Toxoplasma gondii
Kyoung Ju Song, Hye-Jin Ahn, Ho-Woo Nam
Korean J Parasitol 2012;50(1):1-6.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.1

Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.

Citations

Citations to this article as recorded by  Crossref logo
  • Identification and functional analysis of a serine protease inhibitor using machine learning strategy
    Heqian Zhang, Yaxin Wu, Yanran Zhu, Liangjun Ge, Jiaquan Huang, Zhiwei Qin
    International Journal of Biological Macromolecules.2024; 265: 130852.     CrossRef
  • Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells
    Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh
    Pathogens.2023; 12(2): 253.     CrossRef
  • Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains
    Melissa Martins Oliveira, Camila Ramalho Bonturi, Bruno Ramos Salu, Maria Luiza Vilela Oliva, Renato Arruda Mortara, Cristina Mary Orikaza
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • BEWO trophoblast cells and Toxoplasma gondii infection modulate cell death mechanisms in THP-1 monocyte cells by interference in the expression of death receptor and intracellular proteins
    Andressa da Silva Castro, Mariana Bodini Angeloni, Bellisa de Freitas Barbosa, Renata Lima de Miranda, Samuel Cota Teixeira, Pâmela Mendonça Guirelli, Fernanda Chaves de Oliveira, Rafaela José da Silva, Priscila Silva Franco, Mayara Ribeiro, Iliana Claudi
    Tissue and Cell.2021; 73: 101658.     CrossRef
  • Transcriptional profiling of human macrophages during infection withBordetella pertussis
    Denisa Petráčková, Mariam R. Farman, Fabian Amman, Irena Linhartová, Ana Dienstbier, Dilip Kumar, Jakub Držmíšek, Ivo Hofacker, Maria Eugenia Rodriguez, Branislav Večerek
    RNA Biology.2020; 17(5): 731.     CrossRef
  • Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors
    Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam
    The Korean Journal of Parasitology.2020; 58(3): 249.     CrossRef
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    Nour Mammari, Mohamad Adnan Halabi, Souha Yaacoub, Hilda Chlala, Marie-Laure Dardé, Bertrand Courtioux
    BioMed Research International.2019; 2019: 1.     CrossRef
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    Tatiane S. Lima, Melissa B. Lodoen
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
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    Wanbo Zhu, Jingyang Li, Faustina Pappoe, Jilong Shen, Li Yu
    Frontiers in Microbiology.2019;[Epub]     CrossRef
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    Ling Lv, Bing Huang, Qiping Zhao, Zongping Zhao, Hui Dong, Shunhai Zhu, Ting Chen, Ming Yan, Hongyu Han
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium
    Linlu Qi, Mo Hu, Jiaqi Fu, Yanhua Liu, Mei Wu, Kaiwen Yu, Xiaoyun Liu
    PROTEOMICS.2017;[Epub]     CrossRef
  • Phosphoproteome of Toxoplasma gondii Infected Host Cells Reveals Specific Cellular Processes Predominating in Different Phases of Infection
    Hai-Xia Wei, Ai-Yuan Chen, Cheng He, Hong-Juan Peng, Xiao-Shuang Feng
    The American Journal of Tropical Medicine and Hygiene.2017; 97(1): 236.     CrossRef
  • Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response
    Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt
    Frontiers in Immunology.2017;[Epub]     CrossRef
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    Anne M. Brauweiler, Elena Goleva, Donald Y.M. Leung
    Journal of Investigative Dermatology.2016; 136(3): 658.     CrossRef
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    Umasundari Sivaprasad, Kayla G. Kinker, Mark B. Ericksen, Mark Lindsey, Aaron M. Gibson, Stacey A. Bass, Nicolas S. Hershey, Jingyuan Deng, Mario Medvedovic, Gurjit K. Khurana Hershey
    Journal of Investigative Dermatology.2015; 135(1): 160.     CrossRef
  • Trophoblast-macrophage crosstalk on human extravillous under Toxoplasma gondii infection
    P.M. Guirelli, M.B. Angeloni, B.F. Barbosa, A.O. Gomes, A.S. Castro, P.S. Franco, R.J. Silva, J.G. Oliveira, O.A. Martins-Filho, J.R. Mineo, F. Ietta, E.A. Ferro
    Placenta.2015; 36(10): 1106.     CrossRef
  • Toxoplasma gondii inhibits apoptosis via a novel STAT3-miR-17–92-Bim pathway in macrophages
    Yihong Cai, He Chen, Xuwei Mo, Yuanyuan Tang, Xiucai Xu, Aimei Zhang, Zhaorong Lun, Fangli Lu, Yong Wang, Jilong Shen
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  • The role of STAT-6 as a key transcription regulator in HeLa cell death induced by IFN-γ/TNF-α co-immobilized on nanoparticles
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    PLoS ONE.2014; 9(8): e104935.     CrossRef
  • Risk factors associated with seroprevalence of Neospora caninum in dogs from urban and rural areas of milk and coffee production in Minas Gerais state, Brazil
    C. I. NOGUEIRA, L. P. MESQUITA, C. C. ABREU, K. Y. R. NAKAGAKI, J. N. SEIXAS, P. S. BEZERRA, C. M. B. M. ROCHA, A. M. GUIMARAES, A. P. PECONICK, M. S. VARASCHIN
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  • In-depth proteomic analysis of the human cerumen—A potential novel diagnostically relevant biofluid
    Martin Andreas Feig, Elke Hammer, Uwe Völker, Nico Jehmlich
    Journal of Proteomics.2013; 83: 119.     CrossRef
  • Serpins, Immunity and Autoimmunity: Old Molecules, New Functions
    Mariele Gatto, Luca Iaccarino, Anna Ghirardello, Nicola Bassi, Patrizia Pontisso, Leonardo Punzi, Yehuda Shoenfeld, Andrea Doria
    Clinical Reviews in Allergy & Immunology.2013; 45(2): 267.     CrossRef
  • STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage
    Yihong Cai, He Chen, Lei Jin, Yibo You, Jilong Shen
    Parasites & Vectors.2013;[Epub]     CrossRef
  • Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
    M.B. Angeloni, P.M. Guirelli, P.S. Franco, B.F. Barbosa, A.O. Gomes, A.S. Castro, N.M. Silva, O.A. Martins-Filho, T.W.P. Mineo, D.A.O. Silva, J.R. Mineo, E.A.V. Ferro
    Placenta.2013; 34(11): 973.     CrossRef
  • Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses
    ZHI-LIANG DUAN, ZHI-BIN WANG, JIANG-LONG GUO, WEN-QUAN LIU, JUN HU, JING LI, SI-NA WANG, QIANG LI, JIN-SHENG WEN
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Gene Expression Profiles in Genetically Different Mice Infected with Toxoplasma gondii: ALDH1A2, BEX2, EGR2, CCL3 and PLAU
Hassan Ahmed Hassan Ahmed Ismail, Juan-Hua Quan, Zhou Wei, In-Wook Choi, Guang-Ho Cha, Dae-Whan Shin, Young-Ha Lee, Chang-June Song
Korean J Parasitol 2012;50(1):7-13.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.7

Toxoplasma gondii can modulate host cell gene expression; however, determining gene expression levels in intermediate hosts after T. gondii infection is not known much. We selected 5 genes (ALDH1A2, BEX2, CCL3, EGR2 and PLAU) and compared the mRNA expression levels in the spleen, liver, lung and small intestine of genetically different mice infected with T. gondii. ALDH1A2 mRNA expressions of both mouse strains were markedly increased at day 1-4 postinfection (PI) and then decreased, and its expressions in the spleen and lung were significantly higher in C57BL/6 mice than those of BALB/c mice. BEX2 and CCR3 mRNA expressions of both mouse strains were significantly increased from day 7 PI and peaked at day 15-30 PI (P<0.05), especially high in the spleen liver or small intestine of C57BL/6 mice. EGR2 and PLAU mRNA expressions of both mouse strains were significantly increased after infection, especially high in the spleen and liver. However, their expression patterns were varied depending on the tissue and mouse strain. Taken together, T. gondii-susceptible C57BL/6 mice expressed higher levels of these 5 genes than did T. gondii-resistant BALB/c mice, particularly in the spleen and liver. And ALDH1A2 and PLAU expressions were increased acutely, whereas BEX2, CCL3 and EGR2 expressions were increased lately. Thus, these demonstrate that host genetic factors exert a strong impact on the expression of these 5 genes and their expression patterns were varied depending on the gene or tissue.

Citations

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  • Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli
    Xiaoxi Liu, Fenghua Liu, Yunfei Ma, Huanrong Li, Xianghong Ju, Jianqin Xu
    Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1.     CrossRef
  • Gene Silencing of Porcine MUC13 and ITGB5: Candidate Genes towards Escherichia coli F4ac Adhesion
    Chuanli Zhou, Zhengzhu Liu, Yang Liu, Weixuan Fu, Xiangdong Ding, Jianfeng Liu, Ying Yu, Qin Zhang, Lucia R. Languino
    PLoS ONE.2013; 8(7): e70303.     CrossRef
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A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran
Afsaneh Motevalli Haghi, Mohammad Reza Khoramizade, Mehdi Nateghpour, Mehdi Mohebali, Gholam Hossein Edrissian, Mohammad Reza Eshraghian, Zargham Sepehrizadeh
Korean J Parasitol 2012;50(1):15-21.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.15

In Iran, Plasmodium vivax is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic methods. Recombinant P. vivax apical membrane antigen-1 (rPvAMA-1) has been reported to achieve designing rapid, sensitive, and specific molecular diagnosis. This study aimed to perform isolation and expression of a rPvAMA-1, derived from Iranian patients residing in an endemic area. Then, the diagnostic efficiency of the characterized Iranian PvAMA-1 was assessed using an indirect ELISA method. For this purpose, a partial region of AMA-1 gene was amplified, cloned, and expressed in pET32a plasmid. The recombinant His-tagged protein was purified and used to coat the ELISA plate. Antibody detection was assessed by indirect ELISA using rPvAMA-1. The validity of the ELISA method for detection of anti-P. vivax antibodies in the field was compared to light microscopy on 84 confirmed P. vivax patients and compared to 84 non-P. vivax infected individuals. The ELISA cut-off value was calculated as the mean+2SD of OD values of the people living in malaria endemic areas from a south part of Iran. We found a cut-off point of OD=0.311 that showed the best correlation between the sera confirmed with P. vivax infection and healthy control sera. A sensitivity of 81.0% and specificity of 84.5% were found at this cut off titer. A good degree of statistical agreement was found between ELISA using rPvAMA-1 and light microscopy (0.827) by Kappa analysis.

Citations

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  • Immunogenic and diagnostic potential of recombinant apical membrane antigen-1 from Plasmodium malariae
    Moyan Li, Tingting Liu, Yuerong Wang, Luwen Zhang, Fanbo Lu, Jinxing Xia, Meijuan Zheng, Min Zhang, Bo Wang, Yuanhong Xu
    Diagnostic Microbiology and Infectious Disease.2024; 110(3): 116480.     CrossRef
  • A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
    Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding
    Clinical Infectious Diseases.2022; 74(1): 40.     CrossRef
  • Structure-genetic diversity and recombinant protein of circumsporozoite protein (CSP) of vivax malaria antigen: A potential malaria vaccine candidate
    Vahid Raissi, Soudabeh Etemadi, Muhammad Ibrahim Getso, Ahmad Mehravaran, Omid Raiesi
    Gene Reports.2021; 23: 101132.     CrossRef
  • Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients
    Mehdi Nateghpour, Soudabeh Etemadi, Afsaneh Motevalli Haghi, Hamid Eslami, Mehdi Mohebali, Leila Farivar
    European Journal of Medical Research.2021;[Epub]     CrossRef
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    Lis R. Antonelli, Caroline Junqueira, Joseph M Vinetz, Douglas T. Golenbock, Marcelo U. Ferreira, Ricardo T. Gazzinelli
    Immunological Reviews.2020; 293(1): 163.     CrossRef
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    Camilla V. Pires, Jessica R. S. Alves, Barbara A. S. Lima, Ruth B. Paula, Helena L. Costa, Leticia M. Torres, Taís N. Sousa, Irene S. Soares, Bruno A. M. Sanchez, Cor J. F. Fontes, Francis B. Ntumngia, John H. Adams, Flora S. Kano, Luzia H. Carvalho, Gerh
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    Carolina López, Yoelis Yepes-Pérez, Natalia Hincapié-Escobar, Diana Díaz-Arévalo, Manuel A. Patarroyo
    Frontiers in Immunology.2017;[Epub]     CrossRef
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    Parthasarathy Sonaimuthu, Fei Wen Cheong, Lit Chein Chin, Rohela Mahmud, Mun Yik Fong, Yee Ling Lau
    Experimental Parasitology.2015; 153: 118.     CrossRef
  • Immunogenicity of bacterial-expressed recombinant Plasmodium knowlesi merozoite surface protein-142 (MSP-142)
    Fei Wen Cheong, Mun Yik Fong, Yee Ling Lau, Rohela Mahmud
    Malaria Journal.2013;[Epub]     CrossRef
  • Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-133 for Detection of Human Malaria
    Yee Ling Lau, Fei Wen Cheong, Rohela Mahmud, Mun Yik Fong
    The American Journal of Tropical Medicine and Hygiene.2013; 88(5): 835.     CrossRef
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Transmission of Toxocara canis via Ingestion of Raw Cow Liver: A Cross-Sectional Study in Healthy Adults
Dongil Choi, Jae Hoon Lim, Dong-Chull Choi, Kyung Soo Lee, Seung Woon Paik, Sun-Hee Kim, Yoon-Ho Choi, Sun Huh
Korean J Parasitol 2012;50(1):23-27.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.23

The aim of this study is to ascertain the relationship between ingestion of raw cow liver and Toxocara canis infection. A total of 150 apparently healthy adults were divided into 2 groups; 1 group consisted of 86 adults with positive results of Toxocara ELISA, and the other group of 64 adults with negative results. One researcher collected the history of ingestion of raw cow liver within 1 year and recent history of keeping dogs. Among 86 seropositive adults for T. canis, 68 (79.1%) had a recent history of ingestion of raw cow liver. Multivariate statistical analysis showed that a recent ingestion of raw cow liver and keeping dogs were related to an increased risk of toxocariasis (odds ratios, 4.4 and 3.7; and 95% confidence intervals, 1.9-10.2 and 1.2-11.6, respectively). A recent history of ingestion of raw cow liver and keeping dogs was significantly associated with toxocariasis.

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    Jin-woo Kwon, Sun Young Lee, Donghyun Jee, Yang kyung Cho, Der-Chong Tsai
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    Tae Hyung Kim, Doo Hyun Ko, Jong Wook Kim, Ja Joong Gu, Ji Hong Oh, Baek Hyun Yoon, Jae Beom Lee
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    G.-L. YANG, X.-X. ZHANG, C.-W. SHI, W.-T. YANG, Y.-L. JIANG, Z.-T. WEI, C.-F. WANG, Q. ZHAO
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    Gabriela Torres Mattos, Paula Costa dos Santos, Paula de Lima Telmo, Maria Elisabeth Aires Berne, Carlos James Scaini
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    Pam V. Gyang, Olaoluwa P. Akinwale, Yueh-Lun Lee, Ting-Wu Chuang, Akwaowo B. Orok, Olusola Ajibaye, Chien-Wei Liao, Po-Ching Chen, Chia-Mei Chou, Ying-Chieh Huang, Ursula Barghouth, Chia-Kwung Fan
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    The Korean Journal of Parasitology.2014; 52(5): 537.     CrossRef
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    Bahador Sarkari, Malihe Lari, Reza Shafiei, Seyed Mahmoud Sadjjadi
    Archives of Pediatric Infectious Diseases.2014;[Epub]     CrossRef
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    Chung-Jung Fu, Ting-Wu Chuang, Huei-Shan Lin, Chih-Hsiung Wu, Yung-Ching Liu, Mailynn K Langinlur, Min-Yun Lu, Wesley Wei-Wen Hsiao, Chia-Kwung Fan
    BMC Infectious Diseases.2014;[Epub]     CrossRef
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    Wei Cong, Xiao-Xuan Zhang, Na Zhou, Chang-Zheng Yu, Jia Chen, Xiang-Yang Wang, Bing Li, Ai-Dong Qian, Xing-Quan Zhu, Xiao-Nong Zhou
    PLoS Neglected Tropical Diseases.2014; 8(8): e3082.     CrossRef
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    The American Journal of Tropical Medicine and Hygiene.2014; 90(4): 670.     CrossRef
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    Nguyen Van De, Nguyen Vu Trung, Le Van Duyet, Jong-Yil Chai
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    Adrián Bolívar-Mejía, Alfonso J. Rodríguez-Morales, Alberto E. Paniz-Mondolfi, Olinda Delgado
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    Calum N.L. Macpherson
    International Journal for Parasitology.2013; 43(12-13): 999.     CrossRef
  • Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
    Yan Jin, Chenghua Shen, Sun Huh, Woon-Mok Sohn, Min-Ho Choi, Sung-Tae Hong
    The Korean Journal of Parasitology.2013; 51(4): 433.     CrossRef
  • A Case of Toxocariasis with Visceral Larva Migrans Combined with Ocular Larva Migrans
    Ji Eun Park, Mi-Jung Oh, Dong Hyun Oh, In Myung Oh, Kyoung Hwa Yoo, Sung Gyu Im, Hyun Kyung Ghil
    Korean Journal of Medicine.2012; 83(4): 543.     CrossRef
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T Regulatory Cell Responses to Immunization with a Soluble Egg Antigen in Schistosoma mansoni-Infected Mice
Eman El-Ahwany, Ibrahim Rabia Bauiomy, Faten Nagy, Rabab Zalat, Ola Mahmoud, Suher Zada
Korean J Parasitol 2012;50(1):29-35.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.29

The aim of the study is to characterize the phenotypes of CD4+ CD25+ T regulatory cells within the liver granulomas and association with both Foxp-3 gene expression and splenic cytokines. Na?ve C57BL/6 mice were intravenously injected with multiple doses of the soluble egg antigen (SEA) 7 days before cercarial infection. The immunized and infected control groups were sacrificed 8 and 16 weeks post-infection (PI). Histopathology, parasitological parameters, splenic phenotypes for T regulatory cells, the FOXP-3 expression in hepatic granuloma using real-time PCR, and the associated splenic cytokines were studied. Histopathological examination of the liver revealed remarkable increase in degenerated ova within hepatic granuloma which decreased in diameter at weeks 8 and 16 PI (P<0.01). The percentage of T regulatory cells (CD4+ CD25+) increased significantly (P<0.01) in the immunized group compared to the infected control at weeks 8 and 16 PI. The FOXP-3 expression in hepatic granulomas increased from 10 at week 8 to 30 fold at week 16 PI in the infected control group. However, its expression in the immunized group showed an increase from 30 at week 8 to 70 fold at week 16 PI. The splenic cytokine levels of pro-inflammatory cytokines, IFN-γ, IL-4, and TNF-α, showed significant decreases (P<0.05) compared to the infected control group. In conclusion, the magnitude and phenotype of the egg-induced effects on T helper responses were found to be controlled by a parallel response within the T regulatory population which provides protection in worm parasite-induced immunopathology.

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  • Micromorphological Changes in the Parenchymatous Organs of Muskrats Infected with Quinqueserialis quinqueserialis (Trematoda: Notocotilidae)
    O. E. Mazur, A. S. Fomina
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  • Micromorphological changes in the parenchymatous organs of the muskrat infected with Quinqueserialis quinqueserialis (Trematoda: Notocotilidae)
    O. E. Mazur, A. S. Fomina
    Известия Российской академии наук. Серия биологическая.2024;[Epub]     CrossRef
  • Evaluation of schistosomula lung antigen preparation and soluble egg antigen vaccines on experimental schistosomiasis mansoni
    Nagwa S. M. Aly, Hye-Sook Kim, Maysa A. Eraky, Asmaa A. El Kholy, Basma T. Ali, Shin-ichi Miyoshi, Rabab E. Omar
    Parasites, Hosts and Diseases.2023; 61(3): 251.     CrossRef
  • Protective effect and mechanism of Schistosoma japonicum soluble egg antigen against type 1 diabetes in NOD mice
    Li-xia Wang, Yan-ru Gao, Qun Pan, Chun-lian Tang, Rong-hui Zhang, Yan-hong Li, Chun-lan Zheng
    International Journal of Diabetes in Developing Countries.2022; 42(2): 363.     CrossRef
  • Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice
    Youxiang Zhang, De-Hui Xiong, Yangyang Li, Guina Xu, Baoxin Zhang, Yang Liu, Shan Zhang, Qing Huang, Simin Chen, Fansheng Zeng, Jingyi Guo, Bin Li, Zhiqiang Qin, Zuping Zhang, Luiz Felipe Domingues Passero
    Journal of Immunology Research.2021; 2021: 1.     CrossRef
  • Role of regulatory T cells in Schistosoma-mediated protection against type 1 diabetes
    Chun-lian Tang, Yan-ru Gao, Li-xia Wang, Ya-wen Zhu, Qun Pan, Rong-hui Zhang, Ying Xiong
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  • Schistosoma japonicum Soluble Egg Antigen Protects Against Type 2 Diabetes in Leprdb/db Mice by Enhancing Regulatory T Cells and Th2 Cytokines
    Chun-lian Tang, Xiao-hong Yu, Yan Li, Rong-hui Zhang, Jun Xie, Zhi-ming Liu
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    Erica de Souza Fernandes, Virgínia Maria de Barros Lorena, Iana Rafaela Fernandes Sales, Mônica Camelo Pessoa de Azevedo Albuquerque, Yara de Miranda Gomes, Vlaudia Maria Assis Costa, Valdênia Maria Oliveira de Souza
    Revista da Sociedade Brasileira de Medicina Tropical.2018; 51(4): 546.     CrossRef
  • Heat Shock Protein 60 in Eggs Specifically Induces Tregs and Reduces Liver Immunopathology in Mice with Schistosomiasis Japonica
    Sha Zhou, Xin Jin, Xiaojun Chen, Jifeng Zhu, Zhipeng Xu, Xuefeng Wang, Feng Liu, Wei Hu, Liang Zhou, Chuan Su, Susmit Suvas
    PLOS ONE.2015; 10(9): e0139133.     CrossRef
  • Morphological features of cellular responses to different rates of trematode Quinqueserialis quinqueserialis (Trematoda: Notocotilidae) invasion in muskrat (Ondatra zibethicus)
    O. E. Mazur, A. S. Fomina
    Biology Bulletin.2014; 41(5): 444.     CrossRef
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Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice
Ibrahim Rabia Aly, M. Diab, A. M. El-Amir, M. Hendawy, S. Kadry
Korean J Parasitol 2012;50(1):37-43.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.37

Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native F. gigantica FABP in Freund's adjuvant and challenged subcutaneously with 120 Schistosoma mansoni cercariae. Immunization of CD1 mice with F. gigantica FABP has induced heterologous protection against S. mansoni, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed IgG1/IgG2b immune responses with predominant IgG1 isotype, suggesting that native F. gigantica FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native F. gigantica FABP could be a promising vaccine candidate against S. mansoni infection.

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  • Investigating the Taenia solium Fatty Acid Binding Protein Superfamily for Their Immunological Outlook and Prospect for Therapeutic Targets
    Suraj S. Rawat, Gagandeep Singh, Amit Prasad
    ACS Omega.2024; 9(21): 22557.     CrossRef
  • Potential Utility of Systemic Plasma Biomarkers for Evaluation of Pediatric Schistosomiasis in Western Kenya
    Bartholomew N. Ondigo, Rachael E. Hamilton, Edwin O. Magomere, Isaac O. Onkanga, Pauline N. Mwinzi, Maurice R. Odiere, Lisa Ganley-Leal
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • Effect of regulatory T cells on the efficacy of the fatty acid-binding protein vaccine against Schistosoma japonicum
    Chun-lian Tang, Rong-hui Zhang, Zhi-ming Liu, Huang Jin, Long He
    Parasitology Research.2019; 118(2): 559.     CrossRef
  • Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems
    Belén Vicente, Julio López-Abán, Jose Rojas-Caraballo, Esther del Olmo, Pedro Fernández-Soto, Antonio Muro
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Schistosomiasis in Egypt: A never-ending story?
    Ahmad A. Othman, Rasha H. Soliman
    Acta Tropica.2015; 148: 179.     CrossRef
  • Development of the Brazilian Anti Schistosomiasis Vaccine Based on the Recombinant Fatty Acid Binding Protein Sm14 Plus GLA-SE Adjuvant
    Miriam Tendler, Marilia Almeida, Andrew Simpson
    Frontiers in Immunology.2015;[Epub]     CrossRef
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    Gagan Garg, Dolores Bernal, Maria Trelis, Javier Forment, Javier Ortiz, M. Luz Valero, Laia Pedrola, Juan Martinez-Blanch, J. Guillermo Esteban, Shoba Ranganathan, Rafael Toledo, Antonio Marcilla
    Journal of Proteomics.2013; 89: 202.     CrossRef
  • Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein
    Oscar Illescas, Julio C. Carrero, Raúl J. Bobes, Ana Flisser, Gabriela Rosas, Juan P. Laclette
    Molecular and Biochemical Parasitology.2012; 186(2): 117.     CrossRef
  • 9,037 View
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Protective Role of Purified Cysteine Proteinases against Fasciola gigantica Infection in Experimental Animals
Eman EL-Ahwany, Ibrahim Rabia, Faten Nagy, Mona Zoheiry, Tarek Diab, Suher Zada
Korean J Parasitol 2012;50(1):45-51.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.45

Fascioliasis is one of the public health problems in the world. Cysteine proteinases (CP) released by Fasciola gigantica play a key role in parasite feeding, migration through host tissues, and in immune evasion. There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infections. Cysteine proteinases were purified and tested in vaccine trials of sheep infected with the liver fluke. Multiple doses (2 mg of CP in Freund's adjuvant followed by 3 booster doses 1 mg each at 4 week intervals) were injected intramuscularly into sheep 1 week prior to infect orally with 300 F. gigantica metacercariae. All the sheep were humanely slaughtered 12 weeks after the first immunization. Changes in the worm burden, ova count, and humoral and cellular responses were evaluated. Significant reduction was observed in the worm burden (56.9%), bile egg count (70.7%), and fecel egg count (75.2%). Immunization with CP was also found to be associated with increases of total IgG, IgG1, and IgG2 (P<0.05). Data showed that the serum cytokine levels of pro-inflammatory cytokines, IL-12, IFN-γ, and TNF-α, revealed significant decreases (P<0.05). However, the anti-inflammatory cytokine levels, IL-10, TGF-β, and IL-6, showed significant increases (P<0.05). In conclusion, it has been found that CP released by F. gigantica are highly important candidates for a vaccine antigen because of their role in the fluke biology and host-parasite relationships.

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  • Genetic diversity and adaptability of native sheep breeds from different climatic zones
    George Wanjala, Zoltán Bagi, Dinu Gavojdian, Bouabid Badaoui, Putri Kusuma Astuti, Alexandru Mizeranschi, Elena Ilisiu, Husein Ohran, Eva Pasic Juhas, Dimitrios Loukovitis, Aldona Kawęcka, Rūta Šveistienė, Zsolt Becskei, Péter Strausz, Nelly Kichamu, Szil
    Scientific Reports.2025;[Epub]     CrossRef
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    Andleeb Aftab, Rohit Lall, Savita Bisen, Arun Anandanarayanan, Ajayta Rialch, Jayanta K. Chamuah, Shobha Yadav, M. Silamparasan, O. K. Raina
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  • A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice
    Hakim Azizi, Hadi Mirzaeei, Amin Bagheri, Ali Bazi, Ali Khamesipour, Hajar Yaghoobi, Aliyar Mirzapour, Mehrdad Khatami, Samira Elikaee
    Acta Parasitologica.2019;[Epub]     CrossRef
  • Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp ( Ctenopharyngodon idellus ): Induces immune responses and has no damage on liver and intestine function
    Zeli Tang, Hengchang Sun, TingJin Chen, Zhipeng Lin, Hongye Jiang, Xinyi Zhou, Cunbin Shi, Houjun Pan, Ouqin Chang, Pengli Ren, Jinyun Yu, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
    Fish & Shellfish Immunology.2017; 64: 287.     CrossRef
  • Comparative analysis of immune effects in mice model: Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores
    Zhanshuai Wu, Zeli Tang, Mei Shang, Lu Zhao, Lina Zhou, Xiangzhan Kong, Zhipeng Lin, Hengchang Sun, Tingjin Chen, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu
    Parasitology Research.2017; 116(7): 1811.     CrossRef
  • The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease
    Zeli Tang, Mei Shang, Tingjin Chen, Pengli Ren, Hengchang Sun, Hongling Qu, Zhipeng Lin, Lina Zhou, Jinyun Yu, Hongye Jiang, Xinyi Zhou, Xuerong Li, Yan Huang, Jin Xu, Xinbing Yu
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica
    J.M. Martínez-Pérez, D. Robles-Pérez, F.A. Rojo-Vázquez, M. Martínez-Valladares
    Research in Veterinary Science.2014; 97(2): 329.     CrossRef
  • Liver fluke vaccines in ruminants: strategies, progress and future opportunities
    Hayley Toet, David M. Piedrafita, Terry W. Spithill
    International Journal for Parasitology.2014; 44(12): 915.     CrossRef
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Scolicidal Effects of Olea europaea and Satureja khuzestanica Extracts on Protoscolices of Hydatid Cysts
Mohammad Zibaei, Amanallah Sarlak, Bahram Delfan, Behrouz Ezatpour, Alireza Azargoon
Korean J Parasitol 2012;50(1):53-56.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.53

Treatment of hydatid disease is mainly surgical, with medical treatment being reserved as a coadjuvant treatment. Use of effective scolicidal agents during surgery of cystic echinococcosis is essential to reduce the recurrence rate. The goal of this study was to evaluate the in vitro scolicidal effects of hydroalcoholic extracts of Satureja khuzestanica leaves and aqueous extracts of Olea europaea leaves on hydatid cyst protoscolices. Echinococcus granulosus protoscolices were collected from the liver of sheep infected with the hydatid cyst. Various concentrations of plant extracts were used in different exposure times for viability assay of protoscolices. Among the olive leaf extracts tested, 0.1% and 0.01% concentrations had strong scolicidal effects in 120 min. S. khuzestanica 0.1% had very strong scolicidal effects in 30, 60, and 120 min of exposure times and the mortality rate decreased with the lower concentration. The finding have shown that the scolicidal activity of S. khuzestanica against cystic echinococosis protoscolices were more effective, while the O. europaea extract showed less effects.

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    Shiraz E-Medical Journal.2024;[Epub]     CrossRef
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Opportunistic Parasites among Immunosuppressed Children in Minia District, Egypt
Ekhlas H. Abdel-Hafeez, Azza K. Ahmad, Basma A. Ali, Fadia A. Moslam
Korean J Parasitol 2012;50(1):57-62.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.57

A total of 450 stool samples were collected from inpatient and outpatient clinics of Pediatric Department, Minia University Hospital, Minia District, Egypt. Two groups of patients were studied, including 200 immunosuppressed and 250 immunocompetent children. Stool samples were subjected to wet saline and iodine mounts. A concentration technique (formol-ether sedimentation method) was carried out for stool samples diagnosed negative by wet saline and iodine mounts. Samples were stained by 2 different methods; acid fast stain (modified Ziehl-Neelsen stain) and Giemsa stain. Total 188 cases (94%) were diagnosed positive for parasitic infections among immunosuppressed children, whereas 150 cases (60%) were positive in immunocompetent children (P<0.0001). The most common protozoan infection in immunosuppressed group was Cryptosporidium parvum (60.2%), followed by Blastocystis hominis (12.1%), Isospora belli (9.7%), and Cyclospora caytenensis (7.8%). On the other hand, Entamoeba histolytica (24.6%) and Giardia lamblia (17.6%) were more common than other protozoans in immunocompetent children.

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Case Reports

Congenital Neosporosis in Goats from the State of Minas Gerais, Brazil
Mary S. Varaschin, Christian Hirsch, Flademir Wouters, Karen Y. Nakagaki, Ant?nio M. Guimar?es, Domingos S. Santos, Pedro S. Bezerra, Rafael C. Costa, Ana P. Peconick, Ingeborg M. Langohr
Korean J Parasitol 2012;50(1):63-67.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.63

Congenital Neospora caninum infection was diagnosed in two Saanen goat kids from two distinct herds with a history of abortion and weak newborn goat kids in the Southern region of the State of Minas Gerais, Brazil. The first kid was weak at birth, had difficulty to rise and was unable to nurse. Gross lesions of porencephaly and hydrocephalus ex vacuo were seen. Multifocal necrosis, gliosis and non-supurative encephalitis were observed in the brain. Several parasitic cysts with a thick wall that reacted strongly only with polyclonal antiserum to Neospora caninum were seen in the cerebral cortex, brain stem and cerebellum. The second kid was born from a Neospora caninum seropositive mother that aborted in the last pregnancy. It was born without clinical signs. The diagnosis of neosporosis was based on antibody titer of 1:800 to N. caninum by indirect fluorescence antibody test obtained from blood collected before the goat kid ingested the colostrum and Neospora caninum DNA was detected by polymerase chain reaction and sequenced from placenta. This is the first report of neosporosis in goats in the southeast region of Brazil.

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Trichuris vulpis (Froelich, 1789) Infection in a Child: A Case Report
Adrian M?rquez-Navarro, Gudelio Garc?a-Bracamontes, Blanca E. ?lvarez-Fern?ndez, Luz P. ?vila-Caballero, Isabel Santos-Aranda, Dylan L. D?az-Chiguer, Rosa M. S?nchez-Manzano, Elvia Rodr?guez-Bataz, Benjam?n Nogueda-Torres
Korean J Parasitol 2012;50(1):69-71.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.69

We present a human infection with the canine whipworm, Trichuris vulpis, in a child suffering from rhinitis with a diagnosis of rhinitis. T. vulpis eggs resemble those of T. trichiura but they can be differentiated based on their morphological features and egg size, using micrometry with an ocular micrometer. T. vulpis eggs measured an average of 90 ?m by 44 ?m (range 86-99 ?m by 38-47 ?m). Prevalence of hookworms (28.1%), Toxocara canis (11.8%), and Trichuris vulpis (3.5%) was found in 292 fecal samples of dogs collected at the peri-domicile, which showed that the risk of infection was not only fortuitous. The treatment of canine whipworm infections is similar to that of T. trichiura infection. We recommend differentiation of the 2 species for their epidemiological and prevention implications.

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Imported Intraocular Gnathostomiasis with Subretinal Tracks Confirmed by Western Blot Assay
Ji Ho Yang, Moosang Kim, Eung Suk Kim, Byoung-Kuk Na, Seung-Young Yu, Hyung-Woo Kwak
Korean J Parasitol 2012;50(1):73-78.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.73

We report a case of intraocular gnathostomiasis diagnosed by western blot assay in a patient with subretinal tracks. A 15-year-old male patient complained of blurred vision in the right eye, lasting for 2 weeks. Eight months earlier, he had traveled to Vietnam for 1 week and ate raw wild boar meat and lobster. His best-corrected visual acuity was 20/20 in both eyes and anterior chamber examination revealed no abnormalities. Fundus examination showed subretinal tracks in the right eye. Fluorescein angiography and indocyanine green angiography showed linear hyperfluorescence of the subretinal lesion observed on fundus in the right eye. Ultrasound examination revealed no abnormalities. Blood tests indicated mild eosinophilia (7.5%), and there was no abnormality found by systemic examinations. Two years later, the patient visited our department again for ophthalmologic evaluation. Visual acuity remained 20/20 in both eyes and the subretinal tracks in the right eye had not changed since the previous examination. Serologic examination was performed to provide a more accurate diagnosis, and the patient's serum reacted strongly to the Gnathostoma nipponicum antigen by western blot assay, which led to a diagnosis of intraocular gnathostomiasis. This is the first reported case of intraocular gnathostomiasis with subretinal tracks confirmed serologically using western blot in Korea.

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Cat Flea Infestation in a Hospital: A Case Report
Muthupalaniappen Leelavathi, Moktar Norhayati, Yin Yin Lee
Korean J Parasitol 2012;50(1):79-82.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.79

Cat flea bite in humans results in extremely pruritic skin lesions. It has been reported to occur among those living in domiciliary accommodation. However, nosocomial infestation with cat flea has not been reported. We hereby report a case of nosocomial infestation of cat flea in a hospital facility. Identification of the parasite, its appropriate eradication, and adequate medical management of the patients resulted in a satisfactory outcome.

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    Veterinary Medicine and Science.2025;[Epub]     CrossRef
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Brief Communications
Effects of Kimchi Extract and Temperature on Embryostasis of Ascaris suum Eggs
Jin-Sung Kim, Dae-Sung Oh, Kyu-Sung Ahn, Sung-Shik Shin
Korean J Parasitol 2012;50(1):83-87.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.83

To determine the effects of kimchi extracts at different temperatures on larval development, Ascaris suum eggs were mixed with soluble part of 7 different brands of commercially available kimchi and preserved at either 5℃ or 25℃ for up to 60 days. A. suum eggs incubated at 25℃ showed marked differences in larval development between kimchi extract and control group. While all eggs in the control group completed embryonation by day 21, only 30% of the eggs in the kimchi extract group became embryonated by day 36 and about 25% never became larvated even at day 60. At 5℃, however, none of the eggs showed larval development regardless of the incubation period or type of mixture group. To determine the survival rate of A. suum eggs that showed no embryonation after being preserved at 5℃, eggs preserved in kimchi extracts for 14, 28, and 60 at 5℃ were re-incubated at 25℃ for 3 weeks in distilled water. While all eggs in the control group became larvated, eggs in the kimchi extract group showed differences in their embryonation rates by the incubation period; 87.4 % and 41.7% of the eggs became embryonated after being refrigerated for 14 days and 28 days, respectively. When refrigerated for 60 days, however, no eggs mixed in kimchi extract showed larval development. Our results indicate that embryogenesis of A. suum eggs in kimchi extract was affected by duration of refrigeration, and that all eggs stopped larval development completely in kimchi kept at 5℃ for up to 60 days.

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    Min-Ki Kim, Kyoung-Ho Pyo, Young-Sang Hwang, Ki Hwan Park, In Gyun Hwang, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2012; 50(3): 239.     CrossRef
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Depressed Neuronal Growth Associated Protein (GAP)-43 Expression in the Small Intestines of Mice Experimentally Infected with Neodiplostomum seoulense
Kyoung-Ho Pyo, Eun-Young Kang, Bong-Kwang Jung, Jung-Ho Moon, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2012;50(1):89-93.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.89

Neodiplostomum seoulense (Digenea: Neodiplostomidae) is an intestinal trematode that can cause severe mucosal pathology in the small intestines of mice and even mortality of the infected mice within 28 days after infection. We observed neuronal growth associated protein-43 (GAP-43) expression in the myenteric plexus of the small intestinal wall of N. seoulense-infected mice until day 35 post-infection (PI). BALB/c mice were infected with 200 or 500 N. seoulense metacercariae isolated from naturally infected snakes and were killed every 7 days for immunohistochemical demonstration of GAP-43 in the small intestines. N. seoulense-infected mice showed remarkable dilatation of intestinal loops compared with control mice through days 7-28 PI. Conversely, GAP-43 expression in the mucosal myenteric plexus was markedly (P<0.05) reduced in the small intestines of N. seoulense-infected mice during days 7-28 PI and was slightly normalized at day 35 PI. From this study, it is evident that neuronal damage occurs in the intestinal mucosa of N. seoulense-infected mice. However, the correlation between intestinal pathology, including the loop dilatation, and depressed GAP-43 expression remains to be elucidated.

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