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Volume 53(1); February 2015

Mini Review

Serine Proteases of Parasitic Helminths
Yong Yang, Yun jun Wen, Ya Nan Cai, Isabelle Vall?e, Pascal Boireau, Ming Yuan Liu, Shi Peng Cheng
Korean J Parasitol 2015;53(1):1-11.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.1

Serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. A number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. In this review, we described the serine proteases that have been identified in parasitic helminths, including nematodes (Trichinella spiralis, T. pseudospiralis, Trichuris muris, Anisakis simplex, Ascaris suum, Onchocerca volvulus, O. lienalis, Brugia malayi, Ancylostoma caninum, and Steinernema carpocapsae), cestodes (Spirometra mansoni, Echinococcus granulosus, and Schistocephalus solidus), and trematodes (Fasciola hepatica, F. gigantica, and Schistosoma mansoni). Moreover, the possible biological functions of these serine proteases in the endogenous biological phenomena of these parasites and in the host-parasite interaction were also discussed.

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Original Articles

Subtype Distribution of Blastocystis in Thai-Myanmar Border, Thailand
Supaluk Popruk, Ruenruetai Udonsom, Khuanchai Koompapong, Aongart Mahittikorn, Teera Kusolsuk, Jiraporn Ruangsittichai, Attakorn Palasuwan
Korean J Parasitol 2015;53(1):13-19.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.13

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

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Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L.
Hossein Mahmoudvand, Fatemeh Ezzatkhah, Fariba Sharififar, Iraj Sharifi, Ebrahim Saedi Dezaki
Korean J Parasitol 2015;53(1):21-27.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.21

Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

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Seropositivity and Serointensity of Toxoplasma gondii Antibodies and DNA among Patients with Schizophrenia
Ainsah Omar, Osman Che Bakar, Nor Fatini Adam, Hakim Osman, Arina Osman, Ahmad Hatim Suleiman, Mohd Rizal Abdul Manaf, Mohd Ikhsan Selamat
Korean J Parasitol 2015;53(1):29-34.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.29

The aim of this cross sectional case control study was to examine the serofrequency and serointensity of Toxoplasma gondii (Tg) IgG, IgM, and DNA among patients with schizophrenia. A total of 101 patients with schizophrenia and 55 healthy controls from Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) were included in this study. The diagnosis of schizophrenia was made based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). The presence of Tg infection was examined using both indirect (ELISA) and direct (quantitative real-time PCR) detection methods by measuring Tg IgG and IgM and DNA, respectively. The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51). However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005). There was no significant difference (P>0.005) in both serointensity of Tg IgG and DNA between patients with schizophrenia and controls. These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

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  • Reconsidering Toxoplasmosis Prevention and Treatment Due to Its Relation to Neuropsychiatric Disturbances
    Fabrizio Bruschi, Silvia Fabiani
    Zoonotic Diseases.2025; 5(2): 8.     CrossRef
  • Seroprevalence and Associated Risk Factors of Toxoplasma gondii in Patients Diagnosed with Schizophrenia: A Case–Control Cross Sectional Study
    Sebastian Grada, Alin Gabriel Mihu, Daniela Adriana Oatis, Constantin Catalin Marc, Liana Maria Chicea, Cristina Petrescu, Alina Maria Lupu, Tudor Rares Olariu
    Biomedicines.2024; 12(5): 998.     CrossRef
  • Seroprevalence and sociodemographic characteristics of Toxoplasma gondii infection in patients with psychiatric disorders in Malaysia
    Alia Maisarah, Suharni Mohamad, Maruzairi Husain, Sarimah Abdullah, Rahmah Noordin
    Acta Tropica.2024; 255: 107241.     CrossRef
  • The Spread of Toxoplasmosis in Women within Al-Rifai District
    Rehab Issa Hashem Suhail, Shimaa Ahmed Mutab Nayef, Teeba Sabah Daham Mohammed, Yaqeen Mohammed Mahmud, Abdalrhman Falah Omar Ali
    European Journal of Medical and Health Research.2024; 2(4): 257.     CrossRef
  • Infección por Toxoplasma gondii como factor de riesgo para desarrollar esquizofrenia: revisión de la literatura
    Felipe Botero Rodríguez, Ana María Zárate, Damaris Cote Martínez, Carlos Gómez Restrepo, Andrés Duarte Osorio
    Universitas Médica.2023;[Epub]     CrossRef
  • Metabolomic Profiling Reveals Common Metabolic Alterations in Plasma of Patients with Toxoplasma Infection and Schizophrenia
    Emelia Osman, Anis Safirah Mohammad Zahariluddin, Shalisah Sharip, Zulkarnain Md Idris, Jen Kit Tan
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  • Association between exposure to toxoplasmosis and major psychiatric disorders: a systematic review
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    International Journal of Environmental Research and Public Health.2020; 17(13): 4809.     CrossRef
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    Mohammed Nasiru Wana, Mohamad Aris Mohd Moklas, Malaika Watanabe, Ngah Zasmy Unyah, Sharif Alhassan Abdullahi, Ashraf Ahmad Issa Alapid, Norshariza Nordin, Rusliza Basir, Roslaini Abd Majid
    Pathogens.2020; 9(7): 576.     CrossRef
  • Depression and Toxoplasma gondii infection: assess the possible relationship through a seromolecular case–control study
    Saber Nasirpour, Farnaz Kheirandish, Shirzad Fallahi
    Archives of Microbiology.2020; 202(10): 2689.     CrossRef
  • Toxoplasmosis in a Cohort of Italian Patients With Bipolar and Psychotic Disorders
    Claudia Del Grande, Elisa Schiavi, Isabella Masci, Margherita Barbuti, Giuseppe Maccariello, Gabriele Massimetti, Fabrizio Bruschi, Liliana Dell'Osso
    Journal of Nervous & Mental Disease.2020; 208(2): 118.     CrossRef
  • Cerebral Malaria and Toxoplasmosis: Could their Concomitant Presentation Worsen Psychotic Condition?
    Mohammed A. Ibrahim, Olayinka Atilola, Aminu Mohammed, Emmanuel J. Awosanya, Ismail A. Odetokun, Aliyu Muhammad, Chinwe U. Chukwudi, Kingsley N. Ukwaja, Oyetunde T. Oyeyemi
    Annals of Science and Technology.2020; 5(2): 24.     CrossRef
  • Serological and molecular detection of Toxoplasma gondii in terrestrial and marine wildlife harvested for food in Nunavik, Canada
    Nicholas Bachand, André Ravel, Patrick Leighton, Craig Stephen, Momar Ndao, Ellen Avard, Emily Jenkins
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Lack of circulating toxoplasma gondii DNA in seropositive patients with bipolar or schizophrenia spectrum disorders
    L. Galli, C. Del Grande, L. Rindi, C. Mangia, V. Mangano, E. Schiavi, I. Masci, B. Pinto, L. Kramer, L. Dell'Osso, F. Bruschi
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    Cosme Alvarado-Esquivel, Sergio Estrada-Martínez, Alma Rosa Pérez-Alamos
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    International Journal for Parasitology: Parasites and Wildlife.2018; 7(3): 391.     CrossRef
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    Farnaz Kheirandish, Hedayat Nazari, Hossein Mahmoudvand, Yaser Yaseri, Mohammad Javad Tarahi, Shirzad Fallahi, Behrouz Ezatpour
    Archives of Clinical Infectious Diseases.2016;[Epub]     CrossRef
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Sensitivity of Plasmodium falciparum to Antimalarial Drugs in Hainan Island, China
Shan-Qing Wang, Guang-Ze Wang, Yu-Chun Li, Feng Meng, Shi-Gan Lin, Zhen-Hu Zhu, Ding-Wei Sun, Chang-Hua He, Xi-Min Hu, Jian-Wei Du
Korean J Parasitol 2015;53(1):35-41.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.35

Pyronaridine and artesunate have been shown to be effective in falciparum malaria treatment. However, pyronaridine is rarely used in Hainan Island clinically, and artesunate is not widely used as a therapeutic agent. Instead, conventional antimalarial drugs, chloroquine and piperaquine, are used, explaining the emergence of chloroquine-resistant Plasmodium falciparum. In this article, we investigated the sensitivity of P. falciparum to antimalarial drugs used in Hainan Island for rational drug therapy. We performed in vivo (28 days) and in vitro tests to determine the sensitivity of P. falciparum to antimalarial drugs. Total 46 patients with falciparum malaria were treated with dihydroartemisinin/piperaquine phosphate (DUO-COTECXIN) and followed up for 28 day. The cure rate was 97.8%. The mean fever clearance time (22.5±10.6 hr) and the mean parasite clearance time (27.3±12.2 hr) showed no statistical significance with different genders, ages, temperatures, or parasite density (P>0.05). The resistance rates of chloroquine, piperaquine, pyronarididine, and artesunate detected in vitro were 71.9%, 40.6%, 12.5%, and 0%, respectively (P<0.0001). The resistance intensities decreased as follows: chloroquine>piperaquine>pyronarididine>artesunate. The inhibitory dose 50 (IC50) was 3.77×10-6 mol/L, 2.09×10-6 mol/L, 0.09×10-6 mol/L, and 0.05×10-6 mol/L, and the mean concentrations for complete inhibition (CIMC) of schizont formation were 5.60×10-6 mol/L, 9.26×10-6 mol/L, 0.55×10-6 mol/L, and 0.07×10-6 mol/L, respectively. Dihydroartemisinin showed a strong therapeutic effect against falciparum malaria with a low toxicity.

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  • Evaluation Algorithm of Volleyball Players’ Competitive Ability Based on the Random Matrix Model
    Tailin Wang, Hua Zheng, Fangshu Li, Nian Jia, Zengliang Cai, Ning Cao
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Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand
Kanchana Rungsihirunrat, Poonuch Muhamad, Wanna Chaijaroenkul, Jiraporn Kuesap, Kesara Na-Bangchang
Korean J Parasitol 2015;53(1):43-49.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.43

The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples (100 μl) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off IC50 of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.

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    Tropical Medicine and Infectious Disease.2024; 9(5): 94.     CrossRef
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    Thanawat Sridapan, Paweesuda Rattanakoch, Kaewkanha Kijprasong, Suttipat Srisutham, Kristan Alexander Schneider
    PLOS ONE.2024; 19(7): e0304337.     CrossRef
  • Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil
    Jacqueline de Aguiar Barros, Fabiana Granja, Rebecca de Abreu-Fernandes, Lucas Tavares de Queiroz, Daniel da Silva e Silva, Arthur Camurça Citó, Natália Ketrin Almeida-de-Oliveira Mocelin, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
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    Malaria Journal.2023;[Epub]     CrossRef
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    Malaria Journal.2023;[Epub]     CrossRef
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    Journal of Tropical Medicine.2023; 2023: 1.     CrossRef
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    Malaria Journal.2023;[Epub]     CrossRef
  • Surveillance of drug resistance molecular markers in Plasmodium vivax before and after introduction of dihydroartemisinin and piperaquine in Thailand: 2009–2019
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    Myat Htut Nyunt, Thinzar Shein, Ni Ni Zaw, Soe Soe Han, Fauzi Muh, Seong-Kyun Lee, Jin-Hee Han, Kyaw Zin Thant, Eun-Taek Han, Myat Phone Kyaw
    Emerging Infectious Diseases.2017; 23(3): 517.     CrossRef
  • Genetic diversity of Plasmodium vivax metacaspase 1 and Plasmodium vivax multi-drug resistance 1 genes of field isolates from Mauritania, Sudan and Oman
    Fatimata Sow, Guillaume Bonnot, Bilal Rabah Ahmed, Sidi Mohamed Diagana, Hachim Kebe, Mohamedou Koita, Ba Malado Samba, Said K. Al-Mukhaini, Majed Al-Zadjali, Seif S. Al-Abri, Osama A. M. Ali, Abdallah M. Samy, Muzamil Mahdi Abdel Hamid, Musab M. Ali Albs
    Malaria Journal.2017;[Epub]     CrossRef
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    Myat Htut Nyunt, Jin-Hee Han, Bo Wang, Khin Myo Aye, Kyin Hla Aye, Seong-Kyun Lee, Ye Htut, Myat Phone Kyaw, Kay Thwe Han, Eun-Taek Han
    Malaria Journal.2017;[Epub]     CrossRef
  • Measuring ex vivo drug susceptibility in Plasmodium vivax isolates from Cambodia
    Suwanna Chaorattanakawee, Chanthap Lon, Soklyda Chann, Kheang Heng Thay, Nareth Kong, Yom You, Siratchana Sundrakes, Chatchadaporn Thamnurak, Sorayut Chattrakarn, Chantida Praditpol, Kritsanai Yingyuen, Mariusz Wojnarski, Rekol Huy, Michele D. Spring, Dou
    Malaria Journal.2017;[Epub]     CrossRef
  • Plasmodium vivax mdr1 genotypes in isolates from successfully cured patients living in endemic and non-endemic Brazilian areas
    Larissa Rodrigues Gomes, Natália Ketrin Almeida-de-Oliveira, Aline Rosa de Lavigne, Suelen Rezende Félix de Lima, Anielle de Pina-Costa, Patrícia Brasil, Cláudio Tadeu Daniel-Ribeiro, Didier Ménard, Maria de Fatima Ferreira-da-Cruz
    Malaria Journal.2016;[Epub]     CrossRef
  • Plasmodium vivax multidrug resistance-1 gene polymorphism in French Guiana
    Emilie Faway, Lise Musset, Stéphane Pelleau, Béatrice Volney, Jessica Casteras, Valérie Caro, Didier Menard, Sébastien Briolant, Eric Legrand
    Malaria Journal.2016;[Epub]     CrossRef
  • Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodium vivax isolates from Republic of Korea military personnel
    Dong-Il Chung, Sookwan Jeong, Sylvatrie-Danne Dinzouna-Boutamba, Hye-Won Yang, Sang-Geon Yeo, Yeonchul Hong, Youn-Kyoung Goo
    Malaria Journal.2015;[Epub]     CrossRef
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  • 38 Web of Science
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Immunopathological Changes in the Brain of Immunosuppressed Mice Experimentally Infected with Toxocara canis
Mohamed M. Eid, Samy I. El-Kowrany, Ahmad A. Othman, Dina I. El Gendy, Eman M. Saied
Korean J Parasitol 2015;53(1):51-58.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.51

Toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of Toxocara canis. The disease could produce cognitive and behavioral disturbances especially in children. Meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. The present study aimed at comparing some of the pathological and immunological alterations in the brain of normal and immunosuppressed mice experimentally infected with T. canis. Therefore, 180 Swiss albino mice were divided into 4 groups including normal (control) group, immunocompetent T. canis-infected group, immunosuppressed group (control), and immunosuppressed infected group. Infected mice were subjected to larval counts in the brain, and the brains from all mice were assessed for histopathological changes, astrogliosis, and IL-5 mRNA expression levels in brain tissues. The results showed that under immunosuppression, there were significant increase in brain larval counts, significant enhancement of reactive gliosis, and significant reduction in IL-5 mRNA expression. All these changes were maximal in the chronic stage of infection. In conclusion, the immunopathological alterations in the brains of infected animals were progressive over time, and were exaggerated under the effect of immunosuppression as did the intensity of cerebral infection.

Citations

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  • Toxocara canis infections in mice: from subtle to severe consequences in 100 weeks
    Nicol Bernardová, Jan Novák, Chia-Kwung Fan, Libuše Kolářová, Marta Chanová
    Journal of Helminthology.2025;[Epub]     CrossRef
  • Using Biocontrol Fungi to Control Helminthosis in Wild Animals: An Innovative Proposal for the Health and Conservation of Species
    Júlia dos Santos Fonseca, Beatriz Bacelar Barbosa, Adolfo Paz Silva, María Sol Arias Vázquez, Cristiana Filipa Cazapal Monteiro, Huarrisson Azevedo Santos, Jackson Victor de Araújo
    Pathogens.2025; 14(8): 775.     CrossRef
  • The potential relationship between Toxocara canis infection and epilepsy in a rat model
    Amina M. Salama, Dina I. Elgendy, Rasha A. Elmahy, Asmaa F. Eltantawy, Monira A. Seleem, Ahmed M. Elgohary, Omaima K. Docmac, Marwa E. F. Al Mwafy, Ahmed A. Almeldin, Mahmoud S. Sharaf
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  • Efficacy of artemether against toxocariasis in mice: parasitological and immunopathological changes in brain, liver, and lung
    Dina I. Elgendy, Rasha A. Elmahy, Alaa Ibrahim Mohamed Amer, Hoda A. Ibrahim, Asmaa Fawzy Eltantawy, Fotouh Rashed Mansour, Amina M. Salama
    Pathogens and Global Health.2024; 118(1): 47.     CrossRef
  • Neurobehavioral Disorders and Pathological Changes in the Brain of Mice Are Caused by Chronic Toxocara canis Larval Invasion with Low to Moderate Inoculum
    Nicol Bernardová, Jan Novák, Petr Horák, Chia-Kwung Fan, Libuše Kolářová
    Acta Parasitologica.2024; 69(4): 1736.     CrossRef
  • Effects of metformin on parasitological, pathological changes in the brain and liver and immunological aspects during visceral toxocariasis in mice
    Amina M. Salama, Rasha A. Elmahy, Hoda A. Ibrahim, Alaa Ibrahim Mohamed Amer, Asmaa Fawzy Eltantawy, Dina I. Elgendy
    Parasitology Research.2023; 122(12): 3213.     CrossRef
  • Cyclosporine A increases the intensity of Toxocara canis infection in swiss mice
    W. D. S. Terto, M. Q. de Moura, J. L. Borchardt, F. D. S. Santos, L. F. da Costa Avila, N. B. Pinheiro, F. P. Leivas Leite, M. M. Villela, M. E. A. Berne
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    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • Diallyl sulfide alleviates cyclophosphamide-induced nephropathic encephalopathy in rats
    Shereen M. Galal, Heba H. Mansour, Abeer A. Elkhoely
    Toxicology Mechanisms and Methods.2020; 30(3): 208.     CrossRef
  • Mesenchymal stem cells combined with albendazole as a novel therapeutic approach for experimental neurotoxocariasis
    E. V. N. Beshay, S. A. El-Refai, G. S. Sadek, A. A. Elbadry, F. H. Shalan, A. F. Afifi
    Parasitology.2020; 147(7): 799.     CrossRef
  • Histopathological characterization of Toxocara canis- and T. cati-induced neurotoxocarosis in the mouse model
    Andrea Springer, Lea Heuer, Elisabeth Janecek-Erfurth, Andreas Beineke, Christina Strube
    Parasitology Research.2019; 118(9): 2591.     CrossRef
  • Developmental stages and viability of Toxocara canis eggs outside the host
    Iman Fathy Abou-El-Naga
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  • Seroprevalence and Risk Factors for Toxocara canis Infection in Serbia During 2015
    Simona Gabrielli, Suzana Tasić-Otašević, Aleksandra Ignjatović, Maurizio Fraulo, Marija Trenkić-Božinović, Stefan Momčilović, Gabriella Cancrini
    Foodborne Pathogens and Disease.2017; 14(1): 43.     CrossRef
  • 12,702 View
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  • 16 Web of Science
  • Crossref
Experimental Life History and Biological Characteristics of Fasciola gigantica (Digenea: Fasciolidae)
Anawat Phalee, Chalobol Wongsawad, Amnat Rojanapaibul, Jong-Yil Chai
Korean J Parasitol 2015;53(1):59-64.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.59

This study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of Fasciola gigantica in experimental mice. Lymnaea auricularia rubiginosa was used as the intermediate host, and Oryza sativa was used for encystment of the metacercariae, while Mus musculus was used as the definitive host for maturation study. Fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation at about 29ºC. Free-swimming miracidia rapidly penetrated into the snail host, and gradually developed into the next larval stages; sporocyst, redia, and daughter redia with cercariae. Fully-developed cercariae were separated from the redia and shed from the snails on day 39 post-infection (PI). Free-swimming cercariae were immediately allowed to adhere to rice plants, and capsules were constructed to protect metacercariae on rice plants. Juvenile worms were detected in intestines of mice at days 3 and 6 PI, but they were found in the bile duct from day 9 PI. Juvenile and adult flukes were recovered from 16 mice experimentally infected with metacercariae, with the average recovery rate of 35.8%. Sexually mature adult flukes were recovered from day 42 PI. It could be confirmed that experimentally encysted metacercariae could infect and develop to maturity in the experimental host. The present study reports for the first time the complete life history of F. gigantica by an experimental study in Thailand. The obtained information can be used as a guide for prevention, elimination, and treatment of F. gigantica at environment and in other hosts.

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  • Knowledge and Awareness of Bovine Fasciolosis Among Dairy Farm Personnel in the Eastern Cape Province, South Africa
    Zuko Mpisana, Mandla Yawa, Mhlangabezi Slayi, Nkululeko Nyangiwe, James Oguttu, Ishmael Festus Jaja
    Parasitologia.2025; 5(3): 33.     CrossRef
  • Prevalence and risk factors of fasciolosis in a bovine population from farms in Taiping, Malaysia
    Naim Che-Kamaruddin, Nur Fazila Saulol Hamid, Lokman Hakim Idris, Ferdaus Mohamat Yusuff, Zulfa Hanan Ashaari, Hasmawati Yahaya, Norhidayu Sahimin, Nur Mahiza Md Isa
    Veterinary Parasitology: Regional Studies and Reports.2024; 49: 100998.     CrossRef
  • Coproprevalence, seroprevalence, and geographic distribution of Fasciola spp. infection in beef and dairy cattle in Pak Chong highland, Nakhon-Ratchasima Province, Northeast Thailand
    Pongsakorn Martviset, Amornrat Geadkaew-Krenc, Pantip Piyatadsananon, Ruttiroj Jirojwong, Pathanin Chantree, Wansika Phadungsil, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Rawipreeya Poomkhokrak, Aree Taylor, Nanthawat Kosa, Rudi Grams
    Parasitology International.2024; 98: 102809.     CrossRef
  • Investigation of Fasciola gigantica in freshwater snail Radix ( Lymnaea ) spp. In the highly parasite-prevalent area of Nakhon Ratchasima Province, Thailand
    Pongsakorn Martviset, Pathanin Chantree, Amornrat Geadkaew-Krenc, Pantip Piyatadsananon, Ruttiroj Jirojwong, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Rawipreeya Poomkhokrak, Nanthawat Kosa, Salisa Chaimon, Bumpenporn Sanannam, Rudi Grams,
    International Journal of Veterinary Science and Medicine.2024; 12(1): 125.     CrossRef
  • Towards the comprehension of fasciolosis (re-)emergence: an integrative overview
    Annia Alba, Antonio A. Vazquez, Sylvie Hurtrez-Boussès
    Parasitology.2021; 148(4): 385.     CrossRef
  • Differential expression of microRNAs and tRNA fragments mediate the adaptation of the liver fluke Fasciola gigantica to its intermediate snail and definitive mammalian hosts
    Rui-Si Hu, Xiao-Xuan Zhang, Qiao-Ni Ma, Hany M. Elsheikha, Muhammad Ehsan, Quan Zhao, Bastian Fromm, Xing-Quan Zhu
    International Journal for Parasitology.2021; 51(5): 405.     CrossRef
  • Identification of Fasciola spp. based on ITS-2 reveals the Fasciola gigantica infection in buffaloes in Nanning city, South China
    Zhengjiao Wu, Jinhui Wang, Zhen Meng, Weikun Jin, Kangxin He, Weiyu Zhang, Wenda Di
    Veterinary Parasitology.2021; 300: 109585.     CrossRef
  • An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae
    Blaine A. Mathison, Sarah G. H. Sapp
    ZooKeys.2021; 1069: 1.     CrossRef
  • Molecular detection of natural infection ofLymnaea(Pseudosuccinea)columella(Gastropoda: Lymnaeidae) withFasciola gigantica(Digenea: Fasciolidae) from two provinces of South Africa
    M.P. Malatji, S. Mukaratirwa
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Adaptive Radiation of the Flukes of the Family Fasciolidae Inferred from Genome-Wide Comparisons of Key Species
    Young-Jun Choi, Santiago Fontenla, Peter U Fischer, Thanh Hoa Le, Alicia Costábile, David Blair, Paul J Brindley, Jose F Tort, Miguel M Cabada, Makedonka Mitreva, Keith Crandall
    Molecular Biology and Evolution.2020; 37(1): 84.     CrossRef
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    Andrés Esteban Pereira, Nelson Uribe, Jean-Pierre Pointier
    Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100408.     CrossRef
  • Prevalence, risk factors, and spatial distribution of Fasciola in carabao and intermediate host in Baybay, Leyte, Philippines
    Harvie P. Portugaliza, Ivy Mae C. Balaso, June Clyde B. Descallar, Eugene B. Lañada
    Veterinary Parasitology: Regional Studies and Reports.2019; 15: 100261.     CrossRef
  • Les helminthoses à tropisme hépatique
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  • Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts
    Xiao-Xuan Zhang, Krystyna Cwiklinski, Rui-Si Hu, Wen-Bin Zheng, Zhao-An Sheng, Fu-Kai Zhang, Hany M. Elsheikha, John P. Dalton, Xing-Quan Zhu
    BMC Genomics.2019;[Epub]     CrossRef
  • Expression profiles of genes involved in TLRs and NLRs signaling pathways of water buffaloes infected with Fasciola gigantica
    Fu-Kai Zhang, Jun-Ling Hou, Ai-Jiang Guo, Ai-Ling Tian, Zhao-An Sheng, Wen-Bin Zheng, Wei-Yi Huang, Hany M. Elsheikha, Xing-Quan Zhu
    Molecular Immunology.2018; 94: 18.     CrossRef
  • Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica
    Fu-Kai Zhang, Xiao-Xuan Zhang, Hany M. Elsheikha, Jun-Jun He, Zhao-An Sheng, Wen-Bin Zheng, Jian-Gang Ma, Wei-Yi Huang, Ai-Jiang Guo, Xing-Quan Zhu
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    Nga Thi NGUYEN, Thinh Cong LE, Minh Duc Co VO, Hoang VAN CAO, Ly Thi NGUYEN, Khanh Thi HO, Quyet Ngoc NGUYEN, Vui Quang TRAN, Yasunobu MATSUMOTO
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  • Serum levels of cytokines in water buffaloes experimentally infected with Fasciola gigantica
    Fu-Kai Zhang, Ai-Jiang Guo, Jun-Ling Hou, Miao-Miao Sun, Zhao-An Sheng, Xiao-Xuan Zhang, Wei-Yi Huang, Hany M. Elsheikha, Xing-Quan Zhu
    Veterinary Parasitology.2017; 244: 97.     CrossRef
  • 24,455 View
  • 396 Download
  • 22 Web of Science
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Identification and Molecular Characterization of Parkin in Clonorchis sinensis
Xuelian Bai, Tae Im Kim, Ji-Yun Lee, Fuhong Dai, Sung-Jong Hong
Korean J Parasitol 2015;53(1):65-75.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.65

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. A cDNA clone of C. sinensis was predicted to encode a polypeptide homologous to parkin (CsParkin) including 5 domains (Ubl, RING0, RING1, IBR, and RING2). The cysteine and histidine residues binding to Zn2+ were all conserved and participated in formation of tertiary structural RINGs. Conserved residues were also an E2-binding site in RING1 domain and a catalytic cysteine residue in the RING2 domain. Native CsParkin was determined to have an estimated molecular weight of 45.7 kDa from C. sinensis adults by immunoblotting. CsParkin revealed E3-ubiquitin ligase activity and higher expression in metacercariae than in adults. CsParkin was localized in the locomotive and male reproductive organs of C. sinensis adults, and extensively in metacercariae. Parkin has been found to participate in regulating mitochondrial function and energy metabolism in mammalian cells. From these results, it is suggested that CsParkin play roles in energy metabolism of the locomotive organs, and possibly in protein metabolism of the reproductive organs of C. sinensis.

Citations

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  • Characterization of a novel organic solute transporter homologue from Clonorchis sinensis
    Yanyan Lu, Won Gi Yoo, Fuhong Dai, Ji-Yun Lee, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
    PLOS Neglected Tropical Diseases.2018; 12(4): e0006459.     CrossRef
  • Molecular and structural characteristics of multidrug resistance-associated protein 7 in Chinese liver fluke Clonorchis sinensis
    Fuhong Dai, Won Gi Yoo, Ji-Yun Lee, Yanyan Lu, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong
    Parasitology Research.2017; 116(3): 953.     CrossRef
  • 14,090 View
  • 98 Download
  • 3 Web of Science
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Xenomonitoring of Different Filarial Nematodes Using Single and Multiplex PCR in Mosquitoes from Assiut Governorate, Egypt
Ahmed Kamal Dyab, Lamia Ahmed Galal, Abeer El-Sayed Mahmoud, Yasser Mokhtar
Korean J Parasitol 2015;53(1):77-83.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.77

Dirofilaria immitis, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differentiation tool, in area where 3 filarial parasites are coexisting. Female mosquitoes were collected from 7 localities in Assiut Governorate, were microscopically identified and divided into pools according to their species and collection site. Detection of W. bancrofti, D. immitis, and D. repens using single PCR was reached followed by multiplex PCR. Usefulness of multiplex PCR was evaluated by testing mosquito pools to know which genera and species are used by filarial parasites as a vector. An overall estimated rate of infection (ERI) in mosquitoes was 0.6%; the highest was Culex spp. (0.47%). W. bancrofti, D. immitis, and D. repens could be simultaneously and differentially detected in infected vectors by using multiplex PCR. Out of 100 mosquito pools, 8 were positive for W. bancrofti (ERI of 0.33%) and 3 pools each were positive for D. immitis and D. repens (ERI 0.12%). The technique showed 100% sensitivity and 98% specificity. El-Nikhila, El-Matiaa villages, and Sahel Seleem district in Assiut Governorate, Egypt are still endemic foci for filarial parasites. Multiplex PCR offers a reliable procedure for molecular xenomonitoring of filariasis within their respective vectors in endemic areas. Therefore, it is recommended for evaluation of mosquito infection after lymphatic filariasis eradication programs.

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  • Current Gaps in Survey Design and Analysis for Molecular Xenomonitoring of Vector‐Borne Neglected Tropical Diseases: A Systematic Review
    Angus McLure, Tilahun Alamnia, Zhiwei Xu, Colleen L. Lau, Helen J. Mayfield
    Tropical Medicine & International Health.2025; 30(9): 893.     CrossRef
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    Mahmoud K.F. El-Sayed, Manal M. El-Shahawi, Yasmeen M. Ali, Doaa R. Abdel-Haleem, Fatma S.M. Abu El-Azm
    Bioorganic Chemistry.2023; 130: 106258.     CrossRef
  • Mosquito Vectors (Diptera: Culicidae) and Mosquito-Borne Diseases in North Africa
    Amira Nebbak, Lionel Almeras, Philippe Parola, Idir Bitam
    Insects.2022; 13(10): 962.     CrossRef
  • The use of molecular xenomonitoring for surveillance of mosquito-borne diseases
    Mary M. Cameron, Anita Ramesh
    Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816.     CrossRef
  • Bancroftian Filariasis Still Endemic in Some Foci in Sohag Governorate, Upper Egypt
    Refaat MA Khalifa, Amal M Ahmed, Mohamed AA Taha, Nasr Eldeen MM Ali, Haitham KA Abd El Samea, Dabbu Jaijyan
    Journal of Parasite Research.2019; 1(1): 1.     CrossRef
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    Peter Nambala, Janelisa Musaya, Kyoko Hayashida, Emmanuel Maganga, Edward Senga, Kelita Kamoto, John Chisi, Chihiro Sugimoto
    Onderstepoort Journal of Veterinary Research.2018;[Epub]     CrossRef
  • Implementing a community vector collection strategy using xenomonitoring for the endgame of lymphatic filariasis elimination
    Sellase Pi-Bansa, Joseph Harold Nyarko Osei, Joannitta Joannides, Maame Esi Woode, David Agyemang, Elizabeth Elhassan, Samuel Kweku Dadzie, Maxwell Alexander Appawu, Michael David Wilson, Benjamin Guibehi Koudou, Dziedzom Komi de Souza, Jürg Utzinger, Dan
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate
    Ahmed K. Dyab, Lamia A. Galal, Abeer E. Mahmoud, Yasser Mokhtar
    The Korean Journal of Parasitology.2016; 54(3): 265.     CrossRef
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    Parasites & Vectors.2015;[Epub]     CrossRef
  • 10,833 View
  • 163 Download
  • 13 Web of Science
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Proteomic Screening of Antigenic Proteins from the Hard Tick, Haemaphysalis longicornis (Acari: Ixodidae)
Young-Ha Kim, Mohammad Saiful slam, Myung-Jo You
Korean J Parasitol 2015;53(1):85-93.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.85

Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis.

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  • Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of Haemaphysalis longicornis (Parthenogenesis)
    Min-Xuan Liu, Xiao-Pei Xu, Fan-Ming Meng, Bing Zhang, Wei-Gang Li, Yuan-Yuan Zhang, Qiao-Ying Zen, Wen-Ge Liu
    Life.2025; 15(1): 59.     CrossRef
  • Haemaphysalis longicornis calreticulin is not an effective molecular tool for tick bite diagnosis and disruption of tick infestations
    Weiqing Zheng, Haijun Hu, Jiafu Jiang, Xiangrong Sun, Renlong Fu, Huiying Tao, Yangqing Liu, Haiying Chen, Hongmei Ma, Shengen Chen
    Veterinary Parasitology.2022; 309: 109775.     CrossRef
  • Trypanosoma cruzi Calreticulin: Immune Evasion, Infectivity, and Tumorigenesis
    Galia Ramírez-Toloza, Eduardo Sosoniuk-Roche, Carolina Valck, Lorena Aguilar-Guzmán, Viviana P. Ferreira, Arturo Ferreira
    Trends in Parasitology.2020; 36(4): 368.     CrossRef
  • Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle
    Pavlina Vechtova, Zoltan Fussy, Radim Cegan, Jan Sterba, Jan Erhart, Vladimir Benes, Libor Grubhoffer
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Comparative Tandem Mass Tag-Based Quantitative Proteomic Analysis of Tachaea chinensis Isopod During Parasitism
    Yingdong Li, Xin Li, Zhibin Han, Weibin Xu, Xiaodong Li, Qijun Chen
    Frontiers in Cellular and Infection Microbiology.2019;[Epub]     CrossRef
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    Margarita Villar, Anabel Marina, José de la Fuente
    Expert Review of Proteomics.2017; 14(3): 211.     CrossRef
  • Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)
    Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu
    The Korean Journal of Parasitology.2015; 53(6): 789.     CrossRef
  • 10,708 View
  • 108 Download
  • 7 Web of Science
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Case Reports

Comorbid Gastric Adenocarcinoma and Gastric and Duodenal Strongyloides stercoralis Infection: A Case Report
An Na Seo, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong, Ohkyoung Kwon, Han-Ik Bae
Korean J Parasitol 2015;53(1):95-99.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.95

Strongyloides stercoralis can cause systemic infection, termed strongyloidiasis, and gastrointestinal ulcer disease in immunocompromised patients. However, to our knowledge, there are no reported cases of comorbid gastric adenocarcinoma and S. stercoralis infection. Here, we report a case of an 81-year-old Korean man who presented with S. stercoralis infection coexisting with early gastric adenocarcinoma (T1aN0M0). S. stercoralis eggs, rhabditiform larvae, and adult females were observed in normal gastric and duodenal crypts. They were also observed in atypical glands representative of adenocarcinoma and adenoma. Preliminary laboratory tests revealed mild neutrophilic and eosinophilic leukocytosis. A routine stool test failed to detect rhabditiform larvae in the patient’s fecal sample; however, S. stercoralis was identified by PCR amplification and 18S rRNA sequencing using genomic DNA extracted from formalin-fixed paraffin-embedded tissues. Postoperatively, the patient had a persistent fever and was treated with albendazole for 7 days, which alleviated the fever. The patient was followed-up by monitoring and laboratory testing for 4 months postoperatively, and no abnormalities were observed thus far. The fact that S. stercoralis infection may be fatal in immunocompromised patients should be kept in mind when assessing high-risk patients.

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    Journal of Zoo and Wildlife Medicine.2025;[Epub]     CrossRef
  • Gastroduodenal Strongyloidiasis Diagnosed during Iron Deficiency Anemia Workup
    Malek Kreidieh, Neville Mobarakai, Sherif Andrawes, Robert Colef, Lara Kreidieh, Hassan Al Moussawi, Jean M. Chalhoub
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    Ahmed Salih Alshewered
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    Young-Ha Lee
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    Neha Sylvia Walter, Shalmoli Bhattacharyya
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    Maryam Fasihi-Karami, Reza Afrisham, Saina Karami, Ehsan Beigzadeh, Forough Kazemi
    Current Cancer Therapy Reviews.2023; 19(2): 96.     CrossRef
  • STRONGYLOIDES STERCORALIS: A RARE CASE DIAGNOSED WITH ESOPHAGEAL SWAB SAMPLE
    Arzu Hazal AYDIN, Buse BAYAZIT GÖZÜKÜÇÜK, Nesrin TURHAN
    Kırıkkale Üniversitesi Tıp Fakültesi Dergisi.2023; 25(1): 166.     CrossRef
  • Is Gastric Involvement by Strongyloides stercoralis in an Immunocompetent Patient a Common Finding? A Case Report and Review of the Literature
    Irene Pecorella, Tom Richard Okello, Gaia Ciardi, David Martin Ogwang
    Acta Parasitologica.2022; 67(1): 94.     CrossRef
  • Seropositivity Rates of Strongyloides stercoralis Antibody in the Southeastern Region of Republic of Korea: A Single-Center Retrospective Study
    Taehwa Kim, Seungjin Lim
    The Korean Journal of Parasitology.2022; 60(3): 181.     CrossRef
  • Strongyloides Hyperinfection Causing Gastrointestinal Bleeding and Bacteremia in an Immunocompromised Patient
    Juan Carlos De la Cruz Mayhua, Bisharah Rizvi
    Cureus.2021;[Epub]     CrossRef
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    Janneke W. Duijster, Eelco Franz, Jacques Neefjes, Lapo Mughini-Gras
    Frontiers in Microbiology.2021;[Epub]     CrossRef
  • Colorectal Cancer Related to Chronic Strongyloides stercoralis Infection
    M. Sava, T. Huynh, A. Frugoli, L. Kong, M. Salehpour, B. Barrows
    Case Reports in Gastrointestinal Medicine.2020; 2020: 1.     CrossRef
  • Phylogenetic Positioning of a Strongyloides stercoralis Isolate Recovered from a Korean Patient and Comparison with Other Asian Isolates
    Jaeho Bae, Mi Jin Jeong, Dong hoon Shin, Hyun Woo Kim, Sung Ho Ahn, Jun Ho Choi, Hak Sun Yu
    The Korean Journal of Parasitology.2020; 58(6): 689.     CrossRef
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    Eric U. Yee, Enoch Kuo, Jeffrey D. Goldsmith
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    Rashmi Thakur, Balbir Bagicha Singh, Prateek Jindal, Rabinder Singh Aulakh, Jatinder Paul Singh Gill
    Acta Tropica.2018; 186: 5.     CrossRef
  • Compensatory gastric stretching following subtotal gastric resection due to gastric adenocarcinoma in a diamond python (Morelia spilota spilota)
    HR Baron, J Šlapeta, SL Donahoe, RTJ Doneley, DN Phalen
    Australian Veterinary Journal.2018; 96(12): 481.     CrossRef
  • Parasite Infection, Carcinogenesis and Human Malignancy
    Hoang van Tong, Paul J. Brindley, Christian G. Meyer, Thirumalaisamy P. Velavan
    EBioMedicine.2017; 15: 12.     CrossRef
  • Gastric Strongyloidiasis: Report of 2 Cases and Brief Review of the Literature
    Rawia Mohamed, Mowafak M. Hamodat, Mousa A. Al-Abbadi
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  • Strongyloidiasis Current Status with Emphasis in Diagnosis and Drug Research
    Tiago Mendes, Karen Minori, Marlene Ueta, Danilo Ciccone Miguel, Silmara Marques Allegretti
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  • A case of adenocarcinoma developed in the small intestine with chronic strongyloidiasis
    Satoshi Ishikawa, Tamaki Maeda, Kimiaki Hattori, Takahiro Watanabe, Takanori Kuramoto, Saori Ueno, Goro Ueno, Tadahiro Yamada, Akifumi Kanazawa, Masahiro Sakaguchi, Yoshitane Tsukamoto
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  • Colorectal Cancer Associated with Strongyloides stercoralis Colitis
    Carmine Catalano, Joshua Aron, Raghav Bansal, Anatoly Leytin
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    Claudia Machicado, Luis A. Marcos
    International Journal of Cancer.2016; 138(12): 2915.     CrossRef
  • 16,996 View
  • 160 Download
  • 24 Web of Science
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First Record of Cosmocephalus obvelatus (Acuariidae) in Common Gulls (Larus canus) from Gangneung, Korea
Se-Min Kim, Bae-Keun Park, Bae Dong Jung, Hyeon-Cheol Kim
Korean J Parasitol 2015;53(1):101-104.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.101

A nematode species belonging to the genus Cosmocephalus was collected from the stomach of 2 common gulls, Larus canus. The common gulls were found dead on the seaside of Gangneung City, the Republic of Korea. The worms were identified and classified by light (LM) and scanning electron microscopy (SEM) on the basis of important taxonomic characters. The nematodes were characterized by a body length 9.1-9.3 mm (males) and 15.5-15.9 mm (females) and cordons recurrent in anterior direction and anastomosing laterally at about the level of anterior quarter of the buccal cavity. The salient bicuspid deirids were located on the posterior to the cordons. Lateral alae were well-developed, extending from the level just posterior of deirids to the level about middle of the body. LM and SEM observations identified the worms as C. obvelatus. This is the first reported case of C. obvelatus infection in common gulls in Korea.

Citations

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  • Morphological and molecular data on acuariid nematodes in European great cormorants (Phalacrocorax carbo sinensis) and pygmy cormorants (Microcarbo pygmaeus)
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  • Helminths of the black-headed gull (Chroicocephalus ridibundus) from breeding colonies in north-central Poland
    Agata N. Stapf, Izabella Rząd, Katarzyna Królaczyk, Piotr Indykiewicz, Wojciech Gruszka
    Scientific Reports.2024;[Epub]     CrossRef
  • Bir Martıda (Larus cachinnans) Türkiye Faunası için İki Yeni Nematod Türü; Cosmocephalus obvelatus (Creplin, 1825) ve Paracuaria adunca (Creplin, 1846)
    Şinasi UMUR, Gökmen Zafer PEKMEZCİ, Cenk Soner BÖLÜKBAŞ, Celil ATEŞ
    Erciyes Üniversitesi Veteriner Fakültesi Dergisi.2019; 16(3): 238.     CrossRef
  • 10,166 View
  • 144 Download
  • 3 Web of Science
  • Crossref
Four Additional Cases of Diphyllobothrium nihonkaiense Infection Confirmed by Analysis of COX1 Gene in Korea
Sang Hyun Park, Hyeong Kyu Jeon, Jin Bong Kim
Korean J Parasitol 2015;53(1):105-108.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.105

Most of the diphyllobothriid tapeworms isolated from human samples in the Republic of Korea (= Korea) have been identified as Diphyllobothrium nihonkaiense by genetic analysis. This paper reports confirmation of D. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the Department of Parasitology, Hallym University College of Medicine, Korea. Analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity with a reference D. nihonkaiense sequence in GenBank. The present report adds 4 cases of D. nihonkaiense infections to the literature, indicating that the dominant diphyllobothriid tapeworm species in Korea is D. nihonkaiense but not D. latum.

Citations

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  • Dietary footprints of a global parasite: diagnosing Dibothriocephalus nihonkaiensis in non-endemic regions
    Wilson G.W. Goh, Jean-Marc Chavatte, Gabriel Z.R. Yan, Yuan Yi Constance Chen, Mark Dhinesh Muthiah, Lionel H.W. Lum
    Gut Pathogens.2025;[Epub]     CrossRef
  • Human diphyllobothriosis in Taiwan: A review of cases and molecular evidence of Dibothriocephalus nihonkaiensis
    Chia-Kwung Fan, Daniel Barčák, Tomáš Scholz, Pasaikou Sonko, Martina Orosová, Kua-Eyre Su, Chun-Chao Chang, Yuarn-Jang Lee, Roman Kuchta, Mikuláš Oros
    Food and Waterborne Parasitology.2023; 33: e00213.     CrossRef
  • Population genetic structure of diphyllobothriid tapeworms (Cestoda: Diphyllobothriidea) parasitising fish in the Baikal Rift Zone
    IA Kutyrev, VA Mordvinov
    Diseases of Aquatic Organisms.2022; 148: 113.     CrossRef
  • Molecular Identification of Diphyllobothrium latum from a Pediatric Case in Taiwan
    Yu-Chin An, Chia-Cheng Sung, Chih-Chien Wang, Hsin-Chung Lin, Kuang-Yao Chen, Fu-Man Ku, Ruei-Min Chen, Mei-Li Chen, Kuo-Yang Huang
    The Korean Journal of Parasitology.2017; 55(4): 425.     CrossRef
  • Four Human Cases of Diphyllobothrium nihonkaiense (Eucestoda: Diphyllobothriidae) in China with a Brief Review of Chinese Cases
    Yu-Chun Cai, Shao-Hong Chen, Hiroshi Yamasaki, Jia-Xu Chen, Yan Lu, Yong-Nian Zhang, Hao Li, Lin Ai, Hai-Ning Chen
    The Korean Journal of Parasitology.2017; 55(3): 319.     CrossRef
  • Molecular Identification of <i>Diphyllobothrium nihonkaiense</i> from 3 Human Cases in Heilongjiang Province with a Brief Literature Review in China
    Weizhe Zhang, Fei Che, Song Tian, Jing Shu, Xiaoli Zhang
    The Korean Journal of Parasitology.2015; 53(6): 683.     CrossRef
  • 9,010 View
  • 65 Download
  • 8 Web of Science
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Diphyllobothrium nihonkaiense Infections in a Family
Young Bin Go, Eun Hye Lee, Jaeeun Cho, Seoyun Choi, Jong-Yil Chai
Korean J Parasitol 2015;53(1):109-112.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.109

Diphyllobothrium latum and Diphyllobothrium nihonkaiense are morphologically similar to each other, and only genetic method can differentiate clearly between the 2 species. A strobila of diphyllobothriid tapeworm discharged from a 7-year-old boy was analyzed to identify the species by mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequencing. He and his family (total 4 persons) ate slices of 3 kinds of raw fish 16 days before visiting our outpatient clinic. All family members complained of abdominal pain and watery diarrhea. They all expelled tapeworm strobilae in their stools. They were treated with a single oral dose of praziquantel and then complained of no more symptoms. The cox1 gene sequencing of the strobila from the boy revealed 99.9% (687/688 bp) similarity with D. nihonkaiense and only 93.2% (641/688 bp) similarity with D. latum. Thus, we assigned this tapeworm as D. nihonkaiense. This is the first report of D. nihonkaiense infection in a family in Korea, and this report includes the 8th pediatric case in Korea. The current report is meaningful because D. nihonkaiense infection within a family is rare.

Citations

Citations to this article as recorded by  Crossref logo
  • Dietary footprints of a global parasite: diagnosing Dibothriocephalus nihonkaiensis in non-endemic regions
    Wilson G.W. Goh, Jean-Marc Chavatte, Gabriel Z.R. Yan, Yuan Yi Constance Chen, Mark Dhinesh Muthiah, Lionel H.W. Lum
    Gut Pathogens.2025;[Epub]     CrossRef
  • Tapeworm Infection Diagnosed after Campylobacter jejuni-induced Enteritis
    Sotaro Ozaka, Ryusuke Soma, Haruhiko Takahashi, Yuta Shimomori, Masahide Fukuda, Koshiro Tsutsumi, Yuka Hirashita, Kensuke Fukuda, Ryo Ogawa, Kazuhiro Mizukami, Yomei Kagoshima, Nozomi Sachi, Naganori Kamiyama, Hideo Hasegawa, Takashi Kobayashi, Masaaki K
    Internal Medicine.2024; 63(21): 2939.     CrossRef
  • Epidemiology ofDiphyllobothrium nihonkaienseDiphyllobothriasis, Japan, 2001–2016
    Hiroshi Ikuno, Shinkichi Akao, Hiroshi Yamasaki
    Emerging Infectious Diseases.2018;[Epub]     CrossRef
  • RETROSPECTIVE ANALYSIS OF THE EPIDEMIOLOGIC LITERATURE, 1990–2015, ON WILDLIFE-ASSOCIATED DISEASES FROM THE REPUBLIC OF KOREA
    Jusun Hwang, Kyunglee Lee, Young-Jun Kim, Jonathan M. Sleeman, Hang Lee
    Journal of Wildlife Diseases.2017; 53(1): 5.     CrossRef
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Brief Communications
Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China
Qing Lin, Xing-Ye Wang, Jian-Wen Chen, Ling Ding, Guang-Hui Zhao
Korean J Parasitol 2015;53(1):113-117.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.113

Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of post-weaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather’s sugar flotation technique and microscopy at×400 magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.

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    Subin Lee, Sang-Hyup Lee, Badriah Alkathiri, Kyung-Duk Min, Dong Hyeon Kwon, Mi-Hye Hwang, Gyu-Tae Jeon, Bok Kyung Ku, Jae-Won Byun, Seung-Hun Lee
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    Laura Garza-Moreno, Celia León, Joaquín Quílez
    Pathogens.2025; 14(7): 665.     CrossRef
  • Infections and genetic diversity of Cryptosporidium spp. and Giardia spp. in small wild mammals on the Eastern Tibetan plateau: public health implications
    Qingqiu Zuo, Zhuoma Bianba, Chuizhao Xue, Hua Liu, Xiaoxue Peng, Hao Zhou, Xiaocheng Zhang, Quan Chen, Mengqing Li, Yang Yang, Yan Zhou, Jianping Cao, Jianhai Yin, Xu Wang, Yujuan Shen
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    Yuancai Chen, Huikai Qin, Yayun Wu, Huiyan Xu, Jianying Huang, Junqiang Li, Longxian Zhang
    Parasitology.2023; 150(6): 531.     CrossRef
  • Outbreak of severe diarrhea due to zoonotic Cryptosporidium parvum and C. xiaoi in goat kids in Chungcheongbuk-do, Korea
    Ah-Young Kim, Badriah Alkathiri, Subin Lee, Kyung-Duk Min, Soochong Kim, Sang-Myeong Lee, Wan-Kyu Lee, Dongmi Kwak, Seung-Hun Lee
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  • The infection and molecular characterization of Cryptosporidium spp. in diarrheic pigs in southern China
    Pei Wang, Sen Li, Yang Zou, Zi-Cheng Du, De-Ping Song, Ping Wang, Xiao-Qing Chen
    Microbial Pathogenesis.2022; 165: 105459.     CrossRef
  • First Characterization and Zoonotic Potential of Cryptosporidium spp. and Giardia duodenalis in Pigs in Hubei Province of China
    Dongfang Li, Han Deng, Yaxin Zheng, Hongyan Zhang, Sen Wang, Lan He, Junlong Zhao
    Frontiers in Cellular and Infection Microbiology.2022;[Epub]     CrossRef
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    Wei Wang, Qing‐Long Gong, Ao Zeng, Ming‐Han Li, Quan Zhao, Hong‐Bo Ni
    Transboundary and Emerging Diseases.2021; 68(3): 1400.     CrossRef
  • Genotyping and zoonotic potential of Cryptosporidium and Enterocytozoon bieneusi in pigs transported across regions in China
    Hua Liu, Hongxia Ni, Jie Xu, Rong Wang, Yongdong Li, Yujuan Shen, Jianping Cao, Jianhai Yin
    Microbial Pathogenesis.2021; 154: 104823.     CrossRef
  • Prevalence and Characterization of Cryptosporidium Species in Tibetan Antelope (Pantholops hodgsonii)
    Si-Yuan Qin, He-Ting Sun, Chuang Lyu, Jun-Hui Zhu, Zhen-Jun Wang, Tao Ma, Quan Zhao, Yun-Gang Lan, Wen-Qi He
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • A retrospective epidemiological analysis of human Cryptosporidium infection in China during the past three decades (1987-2018)
    Aiqin Liu, Baiyan Gong, Xiaohua Liu, Yujuan Shen, Yanchen Wu, Weizhe Zhang, Jianping Cao, Luther A. Bartelt
    PLOS Neglected Tropical Diseases.2020; 14(3): e0008146.     CrossRef
  • High prevalence of Cryptosporidium infection caused by C. scrofarum and C. suis among pigs in Thailand
    Umaporn Thathaisong, Suradej Siripattanapipong, Tawin Inpankaew, Saovanee Leelayoova, Mathirut Mungthin
    Parasitology International.2020; 77: 102122.     CrossRef
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    Meng Qi, Qiyuan Zhang, Chunyan Xu, Ying Zhang, Jinming Xing, Dayong Tao, Junqiang Li, Longxian Zhang
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    Shuangjian Zheng, Dongfang Li, Chunxiang Zhou, Sumei Zhang, Yayun Wu, Yankai Chang, Yuancai Chen, Jianying Huang, Changshen Ning, Gaiping Zhang, Longxian Zhang
    BMC Veterinary Research.2019;[Epub]     CrossRef
  • Occurrence, Molecular Characterization, and Assessment of Zoonotic Risk of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Pigs in Henan, Central China
    Haiyan Wang, Yiqi Zhang, Yayun Wu, Junqiang Li, Meng Qi, Tingwen Li, Jianling Wang, Rongjun Wang, Sumei Zhang, Fuchun Jian, Changshen Ning, Longxian Zhang
    Journal of Eukaryotic Microbiology.2018; 65(6): 893.     CrossRef
  • Presence of zoonotic Cryptosporidium scrofarum, Giardia duodenalis assemblage A and Enterocytozoon bieneusi genotypes in captive Eurasian wild boars (Sus scrofa) in China: potential for zoonotic transmission
    Wei Li, Lei Deng, Kongju Wu, Xiangming Huang, Yuan Song, Huaiyi Su, Yanchun Hu, Hualin Fu, Zhijun Zhong, Guangneng Peng
    Parasites & Vectors.2017;[Epub]     CrossRef
  • Prevalence and risk factors of Cryptosporidium infection in farmed pigs in Zhejiang, Guangdong, and Yunnan provinces, China
    Yang Zou, Jian-Gang Ma, Dong-Mei Yue, Wen-Bin Zheng, Xiao-Xuan Zhang, Quan Zhao, Xing-Quan Zhu
    Tropical Animal Health and Production.2017; 49(3): 653.     CrossRef
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    Yaoyu Feng, Lihua Xiao
    Frontiers in Microbiology.2017;[Epub]     CrossRef
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    A. Kaupke, J. Gawor, A. Rzeżutka, R. Gromadka
    Experimental Parasitology.2017; 182: 22.     CrossRef
  • 14,076 View
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  • 21 Web of Science
  • Crossref
Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China
Guochao Zheng, Wei Hu, Yuanjia Liu, Qin Luo, Liping Tan, Guoqing Li
Korean J Parasitol 2015;53(1):119-124.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.119

The
objective
of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

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  • Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals
    Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan
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Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
Wei-Feng Qian, Wen-Chao Yan, Tian-Qi Wang, Kai Zhai, Li-Fang Han, Chao-Chao Lv
Korean J Parasitol 2015;53(1):125-128.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.125

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.

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High Genetic Variability of Schistosoma haematobium in Mali and Nigeria
Charles Ezeh, Mingbo Yin, Hongyan Li, Ting Zhang, Bin Xu, Moussa Sacko, Zheng Feng, Wei Hu
Korean J Parasitol 2015;53(1):129-134.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.129

Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

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    The Korean Journal of Parasitology.2015; 53(3): 271.     CrossRef
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Intestinal Nematodes from Small Mammals Captured near the Demilitarized Zone, Gyeonggi Province, Republic of Korea
Deok-Gyu Kim, Jae-Hwan Park, Jae-Lip Kim, Bong-Kwang Jung, Sarah Jiyoun Jeon, Hyemi Lim, Mi Youn Lee, Eun-Hee Shin, Terry A. Klein, Heung-Chul Kim, Sung-Tae Chong, Jin-Won Song, Luck-Ju Baek, Jong-Yil Chai
Korean J Parasitol 2015;53(1):135-139.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.135

A total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including Apodemus agrarius (n = 1,400), Microtus fortis (167), Crocidura lasiura (91), Mus musculus (32), Myodes (= Eothenomys) regulus (9), Micromys minutus (6), and Tscherskia (= Cricetulus) triton (3), were live-trapped at US/Republic of Korea (ROK) military training sites near the demilitarized zone (DMZ) of Paju, Pocheon, and Yeoncheon, Gyeonggi Province from December 2004 to December 2009. Small mammals were examined for their intestinal nematodes by necropsy. A total of 1,617 rodents (100%) and 91 (100%) soricomorphs were infected with at least 1 nematode species, including Nippostrongylus brasiliensis, Heligmosomoides polygyrus, Syphacia obvelata, Heterakis spumosa, Protospirura muris, Capillaria spp., Trichuris muris, Rictularia affinis, and an unidentified species. N. brasiliensis was the most common species infecting small mammals (1,060; 62.1%) followed by H. polygyrus (617; 36.1%), S. obvelata (370; 21.7%), H. spumosa (314; 18.4%), P. muris (123; 7.2%), and Capillaria spp. (59; 3.5%). Low infection rates (0.1-0.8%) were observed for T. muris, R. affinis, and an unidentified species. The number of recovered worms was highest for N. brasiliensis (21,623 worms; mean 20.4 worms/infected specimen) followed by S. obvelata (9,235; 25.0 worms), H. polygyrus (4,122; 6.7 worms), and H. spumosa (1,160; 3.7 worms). A. agrarius demonstrated the highest prevalence for N. brasiliensis (70.9%), followed by M. minutus (50.0%), T. triton (33.3%), M. fortis (28.1%), M. musculus (15.6%), C. lasiura (13.2%), and M. regulus (0%). This is the first report of nematode infections in small mammals captured near the DMZ in ROK.

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