There is no effective treatment modality available against different forms of leishmaniasis. Therefore, the aim of this study was to improve the penetration and efficacy of selenium and glucantime coupled with niosomes and compared them with their simple forms alone on in vitro susceptibility assays. In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects (P ≤ 0.001) than the simple drugs when the selectivity index was considered. The gene expression levels of Interleukin (IL-10) significantly decreased, while the level of IL-12 and metacaspase significantly increased (P ≤ 0.001). The results of the present study showed that selenium plus glucantime niosome possess a potent anti-leishmanial effect and enhanced their lethal activity as evidenced by the in vitro experiments.
Citations
Citations to this article as recorded by
A Comprehensive Review on Recent Advances and Patents of Niosomes Sakshi Saharawat, Sushma Verma Recent Patents on Nanotechnology.2025; 19(3): 364. CrossRef
Promising aryl selenoate derivatives as antileishmanial agents and their effects on gene expression Celia Fernández-Rubio, Mercedes Rubio-Hernández, Verónica Alcolea, Aroia Burguete-Mikeo, Paul A. Nguewa, Silvia Pérez-Silanes, Audrey Odom John Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Combinational therapy with Myc decoy oligodeoxynucleotides encapsulated in nanocarrier and X-irradiation on breast cancer cells BEHROOZ JOHARI, MILAD PARVINZAD LEILAN, MAHMOUD GHARBAVI, YOUSEF MORTAZAVI, ALI SHARAFI, HAMED REZAEEJAM Oncology Research.2024; 32(2): 309. CrossRef
Leishmanicidal and immunomodulatory properties of Brazilian green propolis extract (EPP-AF®) and a gel formulation in a pre-clinical model Jéssica Rebouças-Silva, Nathaly Alcazar Amorim, Flávio Henrique Jesus-Santos, Jéssica Aparecida de Lima, Jonilson Berlink Lima, Andresa A. Berretta, Valéria M. Borges Frontiers in Pharmacology.2023;[Epub] CrossRef
Drug Delivery Through Niosomes: A Comprehensive Review with Therapeutic Applications Mishkaat Parveen Izhar, Abdul Hafeez, Poonam Kushwaha, Simrah Journal of Cluster Science.2023; 34(5): 2257. CrossRef
Nanoemulsions for increased penetrability and sustained release of leishmanicidal compounds Darlyn J. García, Maritza Fernández‐Culma, Yulieth A. Upegui, Luz A. Ríos‐Vásquez, Wiston Quiñones, Rogelio Ocampo‐Cardona, Fernando Echeverri, Iván D. Vélez, Sara M. Robledo Archiv der Pharmazie.2023;[Epub] CrossRef
Niosome as an Effective Nanoscale Solution for the Treatment of Microbial Infections Mahmood Barani, Fatemeh Paknia, Maryam Roostaee, Batoul Kavyani, Davood Kalantar-Neyestanaki, Narges Ajalli, Alireza Amirbeigi, Rahul Shivahare BioMed Research International.2023;[Epub] CrossRef
Selenium and protozoan parasitic infections: selenocompounds and selenoproteins potential Sajad Rashidi, Celia Fernández-Rubio, Reza Mansouri, Mohammad Ali-Hassanzadeh, Esmaeel Ghani, Mohammadreza Karimazar, Raúl Manzano-Román, Paul Nguewa Parasitology Research.2022; 121(1): 49. CrossRef
Preparation and evaluation of physicochemical properties and anti-leishmanial activity of zirconium/tioxolone niosomes against Leishmania major Parisa Fatehi chinar, Sina Bahraminejad, Abbas Pardakhty, Iraj Sharifi, Mahdi Ranjbar, Somayyeh Karami-Mohajeri, Fatemeh Sharifi Arabian Journal of Chemistry.2022; 15(10): 104156. CrossRef
Unveiling a New Selenocyanate as a Multitarget Candidate with Anticancer, Antileishmanial and Antibacterial Potential Sandra Ramos-Inza, Andreina Henriquez-Figuereo, Esther Moreno, Melibea Berzosa, Ignacio Encío, Daniel Plano, Carmen Sanmartín Molecules.2022; 27(21): 7477. CrossRef
Drug Encapsulation: Review of Niosomes for Promoting Antimicrobial Activity Tatielle do Nascimento, Denise de Abreu Garófalo, Mariana Sato de Souza Bustamante Monteiro, Ralph Santos-Oliveira, Ana Paula dos Santos Matos, Eduardo Ricci-Júnior Journal of Nanoparticle Research.2022;[Epub] CrossRef
Niosomes-loaded selenium nanoparticles as a new approach for enhanced antibacterial, anti-biofilm, and anticancer activities Abbas Haddadian, Farnoush Falahi Robattorki, Hedieh Dibah, Ali Soheili, Erfan Ghanbarzadeh, Nasrin Sartipnia, Shadi Hajrasouliha, Kamal Pasban, Romina Andalibi, Mojtaba Hedayati Ch, Arezou Azari, Arman Chitgarzadeh, Aliasghar Bagheri Kashtali, Fatemeh Mas Scientific Reports.2022;[Epub] CrossRef
Leishmaniasis and Trace Element Alterations: a Systematic Review Ali Taghipour, Amir Abdoli, Afifeh Ramezani, Ahmad Abolghazi, Mirza Ali Mofazzal Jahromi, Salar Maani, Seyede Manizhe Heidar Nejadi, Sima Rasti, Morteza Shams, Ezatollah Ghasemi Biological Trace Element Research.2021; 199(10): 3918. CrossRef
New Phosphoramidates Containing Selenium as Leishmanicidal Agents Mikel Etxebeste-Mitxeltorena, Daniel Plano, Socorro Espuelas, Esther Moreno, Carlos Aydillo, Antonio Jiménez-Ruiz, Juan Carlos García Soriano, Carmen Sanmartín Antimicrobial Agents and Chemotherapy.2021;[Epub] CrossRef
Niosomes: A review on niosomal research in the last decade Peeyush Bhardwaj, Purnima Tripathi, Rishikesh Gupta, Sonia Pandey Journal of Drug Delivery Science and Technology.2020; 56: 101581. CrossRef
Leishmanial selenoproteins and the host immune system: towards new therapeutic strategies? Sajad Rashidi, Kurosh Kalantar, Paul Nguewa, Gholamreza Hatam Transactions of The Royal Society of Tropical Medicine and Hygiene.2020; 114(7): 541. CrossRef
The potential role of nicotinamide on Leishmania tropica: An assessment of inhibitory effect, cytokines gene expression and arginase profiling Razieh Tavakoli Oliaee, Iraj Sharifi, Mehdi Bamorovat, Alireza Keyhani, Zahra Babaei, Ehsan Salarkia, Rahele Tavakoly, Ahmad Khosravi, Mahshid Mostafavi, Fatemeh Sharifi, Seyed Mohammad Mousavi International Immunopharmacology.2020; 86: 106704. CrossRef
New Amides Containing Selenium as Potent Leishmanicidal Agents Targeting Trypanothione Reductase Mikel Etxebeste-Mitxeltorena, Daniel Plano, Socorro Espuelas, Esther Moreno, Carlos Aydillo, Antonio Jiménez-Ruiz, Juan Carlos García Soriano, Carmen Sanmartín Antimicrobial Agents and Chemotherapy.2020;[Epub] CrossRef
Melting temperature shift (Tm-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a Tm-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5 ? end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of Tm-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The Tm-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of Tm-values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10-6 and 5.28×10-6 ng/μl samples of AceP and AtuP, respectively. The accuracy of Tm-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the Tm-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.
Citations
Citations to this article as recorded by
Genetic characterization of zoonotic hookworms infecting wild felids in northern India Thangam Venkatesan, Rasmita Panda, Anil Kumar Nehra, Hira Ram, M. Karikalan, Devendra Prasad Pateer, Rajat Garg, A. M. Pawde BMC Veterinary Research.2025;[Epub] CrossRef
A novel A > G polymorphism in the intron 2 of TBX3 gene is significantly associated with body size in donkeys Gang Wang, Mei Li, Jun Zhou, Xiaoya An, Fuxia Bai, Yuan Gao, Jie Yu, Haijing Li, Chuzhao Lei, Ruihua Dang Gene.2021; 785: 145602. CrossRef
Cutaneous Larva Migrans Alfonso J. Rodriguez-Morales, Natalia González-Leal, Maria Camila Montes-Montoya, Lorena Fernández-Espíndola, D. Katterine Bonilla-Aldana, José María Azeñas- Burgoa, Juan Carlos Diez de Medina, Verónica Rotela-Fisch, Melany Bermudez-Calderon, Kovy Arteaga Current Tropical Medicine Reports.2021; 8(3): 190. CrossRef
A stray female cat of unknown age, presenting bright red watery diarrhea, was submitted to the Animal and Plant Quarantine Agency for diagnosis. In the small intestines extracted from the necropsied cat, numerous white oval-shaped organisms were firmly embedded in the mucosa and there was thickening of intestinal wall. Histopathological analysis revealed severe necrotizing enteritis, together with atrophied intestinal villi, exfoliated enterocytes, and parasitic worms. Recovered worms were identified as Pharyngostomum cordatum by morphological observation and genetic analysis. Although P. cordatum is known to occur widely in Korea, this is the first clinical description of an infection by P. cordatum causing severe feline enteritis.
Citations
Citations to this article as recorded by
Metabarcoding of pathogenic parasites based on copro-DNA analysis of wild animals in South Korea Jun Ho Choi, Soo Lim Kim, Dong Kyun Yoo, Myung-hee Yi, Singeun Oh, Myungjun Kim, Sohyeon Yun, Tai-Soon Yong, Seongjun Choe, Jong Koo Lee, Ju Yeong Kim Heliyon.2024; 10(9): e30059. CrossRef
Diversity of trematodes from the amphibian anomaly P hotspot: Role of planorbid snails Anton O. Svinin, Igor V. Chikhlyaev, Ivan W. Bashinskiy, Vitaly V. Osipov, Leonid A. Neymark, Alexander Yu. Ivanov, Tamara G. Stoyko, Polina I. Chernigova, Polina K. Ibrogimova, Spartak N. Litvinchuk, Oleg A. Ermakov, Hudson Alves Pinto PLOS ONE.2023; 18(3): e0281740. CrossRef
Euryhelmis squamula (Digenea: Heterophyidae) Recovered from Korean Raccoon Dog, Nyctereutes procyonoides koreensis, in Korea Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park The Korean Journal of Parasitology.2021; 59(3): 303. CrossRef
Trichomoniasis is the most common curable sexually-transmitted infection. Most Trichomonas vaginalis-infected men are asymptomatic and can remain undiagnosed and untreated, and this has been thought to result in chronic persistent prostatic infection. Chronic inflammation is regarded as the major factor in the pathogenesis and progression of benign prostatic hyperplasia (BPH) and prostatic cancer (PCa). The aim of this study is to identify seropositivity to T. vaginalis in men with prostate tumors (BPH or PCa) visited to Hanyang University Hospital. A total of 183 men were enrolled between October 2013 and November 2014. They consisted of 139 with BPH (mean age: 64.0 ± 0.07) and 44 with prostate cancer (mean age: 73.3±0.18). We carried out ELISA to identify the seropositivity to T. vaginalis. Mixed lysate antigen extracted from 8 strains of T. vaginalis was used in the ELISA. Also 58 male outpatients visited to Health Promotion Center in Hanyang University Hospital were evaluated for comparing group. As a results, seropositivity to T. vaginalis in patients with prostatic diseases was 19.7% (BPH: 18.7%, PCa: 22.7%) and it was significantly higher than the 1.7% of the comparing healthy group (P = 0.001). Therefore, prostatic tumor showed higher seropositivity against T. vaginalis than normal men. As far as we know, this is the first report about seroprevalence in prostatic tumor in Korea.
Citations
Citations to this article as recorded by
The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis Alexandra Ibáñez-Escribano, Juan José Nogal-Ruiz Pathogens.2024; 13(2): 126. CrossRef
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China Zhenchao Zhang, Yuhui Sang, Pucheng Wu, Yujia Shang, Lesong Li, Yujuan Duan, Linfei Zhao, Minghui Gao, Lihua Guo, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lixia Hao, Xuefang Mei, Jianbing Mu Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav Parasite Immunology.2023;[Epub] CrossRef
No Association of Trichomonas vaginalis Seropositivity with Advanced Prostate Cancer Risk in the Multiethnic Cohort: A Nested Case-Control Study Michelle Nagata, Anne Tome, Kami White, Lynne R. Wilkens, Song-Yi Park, Loïc Le Marchand, Christopher Haiman, Brenda Y. Hernandez Cancers.2023; 15(21): 5194. CrossRef
Identification of Signature Genes and Construction of an Artificial Neural Network Model of Prostate Cancer Hongye Dong, Xu Wang, Kalidoss Rajakani Journal of Healthcare Engineering.2022; 2022: 1. CrossRef
Multiple pathogens and prostate cancer James S. Lawson, Wendy K. Glenn Infectious Agents and Cancer.2022;[Epub] CrossRef
Trichomonas vaginalis serostatus and prostate cancer risk in Egypt: a case-control study Nora E. Saleh, Samar M. Alhusseiny, Wafaa M. El-Zayady, Engy M. Aboelnaga, Wafaa N. El-beshbishi, Yasser M. Saleh, Hala S. Abou-ElWafa, Samar N. El-Beshbishi Parasitology Research.2021; 120(4): 1379. CrossRef
The Role of Microbial Factors in Prostate Cancer Development—An Up-to-Date Review Karolina Garbas, Piotr Zapała, Łukasz Zapała, Piotr Radziszewski Journal of Clinical Medicine.2021; 10(20): 4772. CrossRef
Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu The Korean Journal of Parasitology.2021; 59(6): 557. CrossRef
Seroprevalence and risk factors of Trichomonas vaginalis among couples in Al-Hamza city-Iraq. Musafer H. Al-Ardi Al-Kufa University Journal for Biology.2021; 13(1): 26. CrossRef
Is Trichomonas vaginalis a Risk Factor for Prostate Cancer? A Systematic Review and Meta-analysis Gianpaolo Perletti, Vittorio Magri, Louise Beckers-Perletti, Alberto Trinchieri, Konstantinos Stamatiou Hellenic Urology.2021; 33(1): 12. CrossRef
IL-6 produced by prostate epithelial cells stimulated with Trichomonas vaginalis promotes proliferation of prostate cancer cells by inducing M2 polarization of THP-1-derived macrophages Ik-Hwan Han, Hyun-Ouk Song, Jae-Sook Ryu, Michael H. Hsieh PLOS Neglected Tropical Diseases.2020; 14(3): e0008126. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65 Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang BMC Infectious Diseases.2020;[Epub] CrossRef
The Crosstalk between Prostate Cancer and Microbiota Inflammation: Nutraceutical Products Are Useful to Balance This Interplay? Felice Crocetto, Mariarosaria Boccellino, Biagio Barone, Erika Di Zazzo, Antonella Sciarra, Giovanni Galasso, Giuliana Settembre, Lucio Quagliuolo, Ciro Imbimbo, Silvia Boffo, Italo Francesco Angelillo, Marina Di Domenico Nutrients.2020; 12(9): 2648. CrossRef
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
Citations
Citations to this article as recorded by
Trikomoniyaz tanısında farklı laboratuvar yöntemlerinin kullanılması ve Trikomoniyaz hastalarında miRNA profilinin belirlenmesi Hasan Turgut, Fadime Eroğlu Dicle Tıp Dergisi.2024; 51(1): 89. CrossRef
Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases Jie Li, Juan Du, Shengzhican Li, Jiali Dong, Jiahan Ying, Yuehao Gu, Jie Lu, Xinyu Zeng, Philip Kear, Daolong Dou, Xiaodan Wang Phytopathology Research.2023;[Epub] CrossRef
A microfluidic-chip-based system with loop-mediated isothermal amplification for rapid and parallel detection of Trichomonas vaginalis and human papillomavirus Zeyin Mao, Anni Deng, Xiangyu Jin, Meng Li, Wenqi Lv, Leyang Huang, Hao Zhong, Han Yang, Shihong Wang, Yixuan Shi, Lei Zhang, Qinping Liao, Guoliang Huang The Analyst.2023; 148(19): 4820. CrossRef
Development of Nested Polymerase Chain Reaction with Novel Specific Primers for Detection of Tritrichomonas muris Infection in Laboratory Mice Hongbo Zhang, Nan Zhang, Jianhua Li, Panpan Zhao, Xin Li, Xiaocen Wang, Xu Zhang, Bao Yuan, Fei Gao, Pengtao Gong, Xichen Zhang Animals.2023; 13(20): 3177. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65 Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang BMC Infectious Diseases.2020;[Epub] CrossRef
Tahali Mendoza-Oliveros, Victor Arana-Arga?ez, Leidi C. Alvare?z-Sa?nchez, Julio Lara-Riegos, Mari?a Elizbeth Alvare?z-Sa?nchez, Julio C. Torres-Romero
Korean J Parasitol 2019;57(1):33-38. Published online February 26, 2019
Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4+ T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.
Citations
Citations to this article as recorded by
Exposure of Tritrichomonas foetus to sublethal doses of metronidazole induces a specific proinflammatory response in murine macrophages Emanuel Ceballos‐Góngora, Julio César Torres‐Romero, Victor Ermilo Arana‐Argáez, María Elizbeth Alvarez‐Sánchez, Karla Acosta‐Viana, Antonio Euan‐Canto, Leidi Cristal Alvarez‐Sánchez Journal of Eukaryotic Microbiology.2024;[Epub] CrossRef
Activation of murine macrophages by membrane proteins from Tritrichomonas foetus grown on iron‐ and calcium‐rich conditions Antonio Euan‐Canto, Julio César Torres‐Romero, María Elizbeth Alvarez‐Sánchez, Victor Ermilo Arana‐Argáez, Karla Acosta‐Viana, Emanuel Ceballos‐Góngora, Laura Vázquez‐Carrillo, Leidi Alvarez‐Sánchez Parasite Immunology.2024;[Epub] CrossRef
In Vitro Activation of Macrophages by an MHC Class II-restricted Trichomonas Vaginalis TvZIP8-derived Synthetic Peptide Victor Ermilo Arana-Argáez, Emanuel Ceballos-Góngora, María Elizbeth Alvarez-Sánchez, Antonio Euan-Canto, Julio Lara-Riegos, Julio César Torres-Romero Immunological Investigations.2022; 51(1): 88. CrossRef
Local cytokine/chemokine profiles in BALB/c and C57BL/6 mice in response to T. vaginalis infection Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav Experimental Parasitology.2022; 239: 108287. CrossRef
The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis Zhenchao Zhang, Xiaoxiao Song, Zhengbo Zhang, Haoran Li, Yujuan Duan, Hao Zhang, Haoran Lu, Chengyang Luo, Mingyong Wang Microbial Pathogenesis.2021; 152: 104750. CrossRef
The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33) Zhenchao Zhang, Yuhua Li, Shuai Wang, Lixia Hao, Yunqing Zhu, Haoran Li, Xiaoxiao Song, Yujuan Duan, Yuhui Sang, Pucheng Wu, Xiangrui Li Frontiers in Microbiology.2020;[Epub] CrossRef
In the indeterminate chronic period of Chagas disease (ChD) the treatment has not been conclusive, because the serological negativization requires many years. This study aims to evaluate the efficacy of nifurtimox (NF) in the treatment of chronic ChD in prolonged follow-up by serological techniques of indirect immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA) IgG comparing 2 groups of patients, treated and non treated. Mann-Whitney test was performed for ELISA and IFA, with significant difference between the groups (P < 0.05). IgG levels were lower in individuals treated compared with untreated patients, indicating chemotherapeutic efficacy in prolonged follow-up.
Citations
Citations to this article as recorded by
Changes in lipid abundance are associated with disease progression and treatment response in chronic Trypanosoma cruzi infection Juan Carlos Gabaldón-Figueira, Albert Ros-Lucas, Nieves Martínez-Peinado, Gavin Blackburn, Irene Losada-Galvan, Elizabeth Posada, Cristina Ballart, Elisa Escabia, Jordi Capellades, Oscar Yanes, María-Jesús Pinazo, Joaquim Gascón, Julio Alonso-Padilla Parasites & Vectors.2024;[Epub] CrossRef
Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study Inés Zulantay, Gabriela Muñoz, Daniela Liempi, Tamara Rozas, María José Manneschi, Catalina Muñoz-San Martín, Carezza Botto-Mahan, Werner Apt, Gonzalo Cabrera Pathogens.2022; 11(7): 787. CrossRef
Differential phenotypic and functional profile of epitope-specific cytotoxic CD8+ T cells in benznidazole-treated chronic asymptomatic Chagas disease patients Adriana Egui, Manuel Carlos López, Inmaculada Gómez, Marina Simón, Manuel Segovia, M. Carmen Thomas Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.2020; 1866(3): 165629. CrossRef
Detection of Trypanosoma cruzi by PCR in adults with chronic Chagas disease treated with nifurtimox Camilo Vergara, Gabriela Muñoz, Gabriela Martínez, Werner Apt, Inés Zulantay, Jude Marek Przyborski PLOS ONE.2019; 14(8): e0221100. CrossRef
Chronic Chagas disease: Quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy Werner Apt, Daniela Carrasco, Cristian Fuentealba, Mauricio Canals, Gabriela Muñoz, Miguel Saavedra, Juan-Paul Castillo, Inés Zulantay Acta Tropica.2019; 200: 105167. CrossRef
Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Vietnam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.
Citations
Citations to this article as recorded by
Molecular detection and genetic diversity of Anaplasma and Ehrlichia species in livestock and dogs in Pakistan Mughees Aizaz Alvi, Talha Javaid, Abu Bakar Yameen, Fakiha Asghar, Aliza Ali, Iqra Zaheer, Yousef Abdal Jalil Fadladdin, Hong-Bin Yan, Wan-Zhong Jia, Muhammad Saqib, Li Li Acta Tropica.2025; 268: 107700. CrossRef
Isolation, Molecular Characterization and In Vitro Propagation of an Anaplasma platys-Like Bacterium in Tick Cells Erica Rodrigues de Matos, Priscilla Nunes dos Santos, Erich Peter Zweygarth, Talys Henrique Assumpção Jardim, Huarrisson Azevedo Santos, Matheus Dias Cordeiro, Bruna de Azevedo Baêta, Lesley Bell-Sakyi, Adivaldo Henrique da Fonseca, Claudia Bezerra da Sil Pathogens.2025; 14(9): 901. CrossRef
Tick distribution and comparative analysis of bovine blood microbiome in two provinces of South Africa using 16S rRNA PacBio sequencing approach Bongekile L. Khoza, Charles Byaruhanga, S. Marcus Makgabo, Nkululeko Nyangiwe, Themba Mnisi, Samukelo Nxumalo, Marinda C. Oosthuizen, Zamantungwa T. H. Mnisi Frontiers in Tropical Diseases.2024;[Epub] CrossRef
Molecular Characterization of Anaplasma spp. in Cattle from Kazakhstan Madina Kadyrova, Alexandr Ostrovskii, Kassym Mukanov, Amirkhan Kassen, Elena Shevtsova, Maxat Berdikulov, Gilles Vergnaud, Alexandr Shevtsov Pathogens.2024; 13(10): 894. CrossRef
Detection of Tick‐Borne Pathogen Coinfections and Coexposures to Foot‐and‐Mouth Disease, Brucellosis, and Q Fever in Selected Wildlife From Kruger National Park, South Africa, and Etosha National Park, Namibia Carlo Andrea Cossu, Sunday Ochonu Ochai, Milana Troskie, Axel Hartmann, Jacques Godfroid, Lin-Mari de Klerk, Wendy Turner, Pauline Kamath, Ockert Louis van Schalkwyk, Rudi Cassini, Raksha Bhoora, Henriette van Heerden, Nan-Hua Chen Transboundary and Emerging Diseases.2024;[Epub] CrossRef
Dynamics of Infections in Cattle and Rhipicephalus microplus: A Preliminary Study Elianne Piloto-Sardiñas, Angélique Foucault-Simonin, Alejandra Wu-Chuang, Lourdes Mateos-Hernández, Roxana Marrero-Perera, Lianet Abuin-Denis, Lisset Roblejo-Arias, Cristian Díaz-Corona, Zbigniew Zając, Joanna Kulisz, Aneta Woźniak, Sara Moutailler, Belki Pathogens.2023; 12(8): 998. CrossRef
Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys in cattle in Nigeria Joshua Kamani, Juliane Schaer, Ali G. Umar, James Y. Pilarshimwi, Laminu Bukar, Javier González-Miguel, Shimon Harrus Ticks and Tick-borne Diseases.2022; 13(4): 101955. CrossRef
Genetic diversity of Anaplasma marginale in calves with anaplasmosis on farms in Minas Gerais, Brazil Michele Bahia, Lucas Teixeira da Silva, Bruno Montijo da Silva, Matheus dias Cordeiro, Alexandro Guterres, Claudia Bezerra da Silva, Jenevaldo Barbosa da Silva, Adivaldo Henrique Fonseca Ticks and Tick-borne Diseases.2021; 12(1): 101552. CrossRef
Comparison of Anaplasma and Ehrlichia species–specific peptide ELISAs with whole organism–based immunofluorescent assays for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs Barbara A. Qurollo, Brett A. Stillman, Melissa J. Beall, Paulette Foster, Barbara C. Hegarty, Edward B. Breitschwerdt, Ramaswamy Chandrashekar American Journal of Veterinary Research.2021; 82(1): 71. CrossRef
Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef
Molecular detection of selected tick-borne pathogens infecting cattle at the wildlife–livestock interface of Queen Elizabeth National Park in Kasese District, Uganda Benedicto Byamukama, Patrick Vudriko, Maria Agnes Tumwebaze, Dickson Stuart Tayebwa, Joseph Byaruhanga, Martin Kamilo Angwe, Jixu Li, Eloiza May Galon, Aaron Ringo, Mingming Liu, Yongchang Li, Shengwei Ji, Mohamed Abdo Rizk, Paul Frank Adjou Moumouni, Seu Ticks and Tick-borne Diseases.2021; 12(5): 101772. CrossRef
Molecular detection of Theileria species, Anaplasma species, Candidatus Mycoplasma haemobos, Trypanosoma evansi and first evidence of Theileria sinensis-associated bovine anaemia in crossbred Kedah-Kelantan x Brahman cattle Onyinyechukwu Ada Agina, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Mazlina Mazlan, Azim Salahuddin Muhamad, Afrah Alhana Kassim, Lee Chai Ha, Fairuz Hazwani Rusli, Darulmuqaamah Masaud, Hazilawati Hamzah BMC Veterinary Research.2021;[Epub] CrossRef
Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam Ly Na Huynh, Adama Zan Diarra, Quang Luan Pham, Nhiem Le-Viet, Jean-Michel Berenger, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola, Lynn Soong PLOS Neglected Tropical Diseases.2021; 15(9): e0009813. CrossRef
The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal Marcos Rogério André, Ana Cláudia Calchi, Heitor Miraglia Herrera, Diego Carlos de Souza Zanatto, Bruna de Carvalho Le Senéchal Horta, Júlia Banhareli Tasso, Inalda Angélica de Souza Ramos, Victória Valente Califre de Mello, Rosangela Zacarias Machado Veterinary Parasitology: Regional Studies and Reports.2020; 21: 100437. CrossRef
Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand Anh H. L. Nguyen, Sonthaya Tiawsirisup, Morakot Kaewthamasorn BMC Veterinary Research.2020;[Epub] CrossRef
Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage.
Citations
Citations to this article as recorded by
Development of a Dielectric Heating System for Selective Thermal Targeting of Liver Fluke Regions in Cirrhinus microlepis Supatinee Kornsing, Sirigiet Phunklang, Chanchai Thongsopa, Piyaporn Krachodnok, Nuchanart Santalunai, Samran Santalunai Applied Sciences.2025; 15(10): 5466. CrossRef
Viability of Trichinella spiralis in traditional sour pork fermentation and its inactivation by microwave heating: Implications for zoonotic risk and food safety Atchara Artchayasawat, Benjamabhorn Pumhirunroj, Sukhonthip Khueangchiangkhwang, Thidarut Boonmars, Parichart Boueroy, Porntip Laummaunwai, Panaratana Rattanasuwan Veterinary World.2025; : 1660. CrossRef
This study was undertaken to determine the complete mitochondrial DNA sequence and structure of the mitochondrial genome of Spirometra ranarum, and to compare it with those of S. erinaceieuropaei and S. decipiens. The aim of this study was to provide information of the species level taxonomy of Spirometra spp. using the mitochondrial genomes of 3 Spirometra tapeworms. The S. ranarum isolate originated from Myanmar. The mitochondrial genome sequence of S. ranarum was compared with that of S. erinaceieuropaei (GenBank no. KJ599680) and S. decipiens (GenBank no. KJ599679). The complete mtDNA sequence of S. ranarum comprised 13,644 bp. The S. ranarum mt genome contained 36 genes comprising 12 protein-coding genes, 22 tRNAs and 2 rRNAs. The mt genome lacked the atp8 gene, as found for other cestodes. All genes in the S. ranarum mitochondrial genome are transcribed in the same direction and arranged in the same relative position with respect to gene loci as found for S. erinaceieuropaei and S. decipiens mt genomes. The overall nucleotide sequence divergence of 12 protein-coding genes between S. ranarum and S. decipiens differed by 1.5%, and 100% sequence similarity was found in the cox2 and nad6 genes, while the DNA sequence divergence of the cox1, nad1, and nad4 genes of S. ranarum and S. decipiens was 2.2%, 2.1%, and 2.6%, respectively.
Citations
Citations to this article as recorded by
Molecular identification of sparganum of Spirometra mansoni isolated from the abdominal cavity of a domestic cat in Vietnam Yen Thi Hoang NGUYEN, Lan Anh Thi NGUYEN, Hieu VAN DONG, Hieu Duc DUONG, Ayako YOSHIDA Journal of Veterinary Medical Science.2024; 86(1): 96. CrossRef
Spirometra species from Asia: Genetic diversity and taxonomic challenges Hiroshi Yamasaki, Oranuch Sanpool, Rutchanee Rodpai, Lakkhana Sadaow, Porntip Laummaunwai, Mesa Un, Tongjit Thanchomnang, Sakhone Laymanivong, Win Pa Pa Aung, Pewpan M. Intapan, Wanchai Maleewong Parasitology International.2021; 80: 102181. CrossRef
Insight into One Health Approach: Endoparasite Infections in Captive Wildlife in Bangladesh Tilak Chandra Nath, Keeseon S. Eom, Seongjun Choe, Shahadat Hm, Saiful Islam, Barakaeli Abdieli Ndosi, Yeseul Kang, Mohammed Mebarek Bia, Sunmin Kim, Chatanun Eamudomkarn, Hyeong-Kyu Jeon, Hansol Park, Dongmin Lee Pathogens.2021; 10(2): 250. CrossRef
Mitochondrial Genome of Spirometra theileri Compared with Other Spirometra Species Barakaeli Abdieli Ndosi, Hansol Park, Dongmin Lee, Seongjun Choe, Yeseul Kang, Tilak Chandra Nath, Mohammed Mebarek Bia, Chatanun Eamudomkarn, Hyeong-Kyu Jeon, Keeseon S. Eom The Korean Journal of Parasitology.2021; 59(2): 139. CrossRef
Low prevalence of spargana infection in farmed frogs in the Yangtze River Delta of China Xiaoli Zhang, Rongsheng Mi, Yehua Zhang, Shijie Zhang, Tao Sun, Haiyan Jia, Yan Huang, Haiyan Gong, Xiangan Han, Zhaoguo Chen Infection, Genetics and Evolution.2020; 85: 104466. CrossRef
Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
Citations
Citations to this article as recorded by
Rapid and non‐invasive detection of cystic echinococcosis in sheep based on serum fluorescence spectrum combined with machine learning algorithms Shengke Xu, Wubulitalifu Dawuti, Maierhaba Maimaitiaili, Jingrui Dou, Malike Aizezi, Kalibixiati Aimulajiang, Xiaoyi Lü, Guodong Lü Journal of Biophotonics.2024;[Epub] CrossRef
Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis Ana Alice Maia Gonçalves, Anna Julia Ribeiro, Carlos Ananias Aparecido Resende, Carolina Alves Petit Couto, Isadora Braga Gandra, Isabelle Caroline dos Santos Barcelos, Jonatas Oliveira da Silva, Juliana Martins Machado, Kamila Alves Silva, Líria Souza Si Microbial Cell Factories.2024;[Epub] CrossRef
Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95 Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao Parasitology.2024; 151(13): 1458. CrossRef
Rapid and accurate screening of cystic echinococcosis in sheep based on serum Fourier‐transform infrared spectroscopy combined with machine learning algorithms Wubulitalifu Dawuti, Jingrui Dou, Xiangxiang Zheng, Xiaoyi Lü, Hui Zhao, Lingfei Yang, Renyong Lin, Guodong Lü Journal of Biophotonics.2023;[Epub] CrossRef
Evaluation of a novel Echinococcus granulosus recombinant fusion B-EpC1 antigen for the diagnosis of human cystic echinococcosis using indirect ELISA in comparison with a commercial diagnostic ELISA kit Enayat Darabi, Elahe Motevaseli, Mehdi Mohebali, Mohammad Bagher Rokni, Mohammad Reza Khorramizadeh, Farzaneh Zahabiun, Soudabeh Heidari, Eshrat Beigom Kia Experimental Parasitology.2022; 240: 108339. CrossRef
Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line Andrea Maglioco, Jorge Gentile, Melisa S. Barbery Venturi, Oscar Jensen, Claudia Hernández, María Laura Gertiser, Verónica Poggio, Gabriela Canziani, Alicia Graciela Fuchs Parasite Immunology.2019;[Epub] CrossRef
In a population-based study with 4 years of follow up, we evaluated the prevalence of Coxiella burnetii in cattle on Ulleung Island, Korea. In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3-1.0% in the period 2011-2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide sequences of the 2 samples showed high (98.4-100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the transmission of C. burnetii on Ulleung Island, control strategy should include biosecurity improvement in surveillance, livestock management, administering suitable tests before purchasing animals to detect C. burnetii shedders, and restricting movements between herds.
Citations
Citations to this article as recorded by
Comprehensive Analysis of SPL Genes in Cucurbitaceae Species: Evolutionary Insights, Functional Diversity and Regulatory Mechanisms PavanKalyan Neelam, Balakishore Lalam, Jaswanth Manavi, Ghanishtha Prusty, Suman Polaki, Pushpalatha Ganesh Iranian Journal of Science.2025; 49(5): 1209. CrossRef
Leaving behind fond memories, I am stepping away from my role as editor of the Ewha Medical Journal after finalizing this issue's theme Sun Huh Ewha Medical Journal.2025; 48(4): e51. CrossRef
Presidential address: the Korean Council of Science Editors as a board member of Crossref from March 2021 to February 2024 Sun Huh Science Editing.2021; 8(1): 1. CrossRef
First
Prev
