Parasites, Hosts and Diseases

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Volume 41(3); September 2003

Original Articles

Prevalence of Theileria sergenti infection in Korean native cattle by polymerase chain reaction: Kun-Ho Song, Byung-Chan Sang; Korean J Parasitol 2003;41(3):141-145.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.141

Abstract
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This study was performed to investigate the prevalence of theileriosis and to compare the prevalence of this disease in Korean native cattle reared under different environmental conditions, namely, in a grazing area and a non-grazing area by polymerase chain reaction. Three hundred and one Korean native cattle (276 cows and 25 bulls) that had not received prior treatment or been vaccinated to prevent theileriosis were examined by PCR for Theileria sergenti infection from 2001 to 2002. In our study, the parasitemia range in T. sergenti-positive cattle by microscopy were from 0.1 to 3% (mean 0.8%). In terms of mean prevalence, 204 of the 301 Korean native cattle (67.8%) were positive reaction by PCR. Our results also revealed that the infection rate among cows (70.3%) was significantly higher than that among bulls (40.0%) (p < 0.01). T. sergenti infection among the over 3 year-old-group (75%) had a significant higher prevalence than that among the less than 3 year-old-group (61.8%) (p < 0.05). Our data also showed that grazing areas (76.1%) had the significant higher prevalence than non-grazing areas (51%) (p < 0.001). In conclusion, this study demonstrates that the prevalence of T. sergenti infection is high and that its prevalence in grazing cattle is higher than that in non-grazing cattle. Therefore, life-long treatment and the development of an optimal vaccine are needed to reduce the numbers of bovine theileriosis in both grazing and non-grazing areas.

Citations

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Prevalence of Anaplasma Spp. and Theileria Spp. Antigens and Antibodies in Housed and Grazing Korean Indigenous Cattle
Ji-Yeong Ku, Youngwoo Jung, Youngjun Kim, Kyoung-Seong Choi, Jinho Park
Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
Molecular epidemiology of Theileria species in ticks and its potential threat to livestock in the Republic of Korea
Badriah Alkathiri, KyuSung Ahn, Haeseung Lee, Yun Sang Cho, So Youn Youn, Min-Goo Seo, Dongmi Kwak, SungShik Shin, Seung-Hun Lee
Acta Tropica.2023; 238: 106780. CrossRef
Putative target sites in synganglion for novel ixodid tick control strategies
Jéssica Waldman, Guilherme Marcondes Klafke, Lucas Tirloni, Carlos Logullo, Itabajara da Silva Vaz
Ticks and Tick-borne Diseases.2023; 14(3): 102123. CrossRef
A case of piroplasmosis treated with acriflavine in a Japanese Black cow
F. Tatsukawa
Japanese Journal of Large Animal Clinics.2017; 7(Supple): 235. CrossRef
Seasonal changes in hemograms and Theileria orientalis infection rates among Holstein cattle pastured in the mountains in the Republic of Korea
Kyoung-Seong Choi, Do-Hyeon Yu, Joon-Seok Chae, Bae-Keun Park, Jae-Gyu Yoo, Jinho Park
Preventive Veterinary Medicine.2016; 127: 77. CrossRef
A survey for tick-borne pathogens in Korean native cattle from northern area of Gyeongbuk
Jae-Cheong Cho, Woo-Jin Jeon, Seon-Soo Kim, Seong-Guk Kim
Korean Journal of Veterinary Service.2016; 39(1): 29. CrossRef
Emergence of oriental theileriosis in cattle and its transmission through Rhipicephalus (Boophilus) microplus in Assam, India
Parikshit Kakati, Prabhat Chandra Sarmah, Debdatta Ray, Kanta Bhattacharjee, Rajeev Kumar Sharma, Luit Moni Barkalita, Dipak Kumar Sarma, Bhaben Chandra Baishya, Pranjal Borah, Bobitha Stanley
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Molecular investigation of tick-borne pathogens in ticks from grazing cattle in Korea
Seung Won Kang, Huong Thi Thanh Doan, Se Eun Choe, Jin Hyeong Noh, Mi Sun Yoo, Kondreddy Eswar Reddy, Young Ha Kim, Chang Hee Kweon, Suk Chan Jung, Ki Yoon Chang
Parasitology International.2013; 62(3): 276. CrossRef
Prevalence of Theileria sergenti infection in cattle of eastern areas in Gyeongbuk province by PCR
Min-Goo Seo, Jae-Cheu Do, Min-Hee Cho, Hee-Jin Seo, Joong-Kew Kim, Young-Hoan Kim, No-Chan Park, Dong-Mi Kwak
Korean Journal of Veterinary Service.2011; 34(3): 251. CrossRef
Loop-mediated isothermal amplification (LAMP) assay for detection of Theileria sergenti infection targeting the p33 gene
L.X. Wang, L. He, R. Fang, Q.Q. Song, P. Tu, A. Jenkins, Y.Q. Zhou, J.L. Zhao
Veterinary Parasitology.2010; 171(1-2): 159. CrossRef
High prevalence of Theileria sp. in wild Chinese Water Deer (Hydropotes inermis argyropus) in South Korea
Jae-Ik Han, Hye-Jin Jang, Sook-Jin Lee, Ki-Jeong Na
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Antigenic diversity ofTheileriamajor piroplasm surface protein gene in Jeju black cattle
Myung-Soon Ko, Kyoung-Kap Lee, Kyu-Kye Hwang, Byung-Sun Kim, Gui-Cheol Choi, Young-Min Yun
Journal of Veterinary Science.2008; 9(2): 155. CrossRef
SEROLOGICAL INVESTIGATION OF THEILERIA SERGENTI USING LATEX AGGLUTINATION TEST IN SOUTH KOREA
Wooseog Jeong, Chang Hee Kweon, Jong Man Kim, Hwan Jang, Sang Gi Paik
Journal of Parasitology.2005; 91(1): 164. CrossRef

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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea: Jong-Yil Chai, Aifen Lin, Eun-Hee Shin, Myoung-Don Oh, Eun-Taek Han, Ho-Woo Nam, Soon-Hyung Lee; Korean J Parasitol 2003;41(3):147-154.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.147

Abstract
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Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.

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Journal of Food Protection.2016; 79(5): 821. CrossRef
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Ocular toxoplasmosis past, present and new aspects of an old disease
M. Maenz, D. Schlüter, O. Liesenfeld, G. Schares, U. Gross, U. Pleyer
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Detection of Ocular Toxoplasma gondii Infection in Chronic Irregular Recurrent Uveitis by PCR
Sang-Eun Lee, Sung-Hee Hong, Seong-Ho Lee, Young-Il Jeong, Su Jin Lim, Oh Woong Kwon, Sun Hyun Kim, Young Sung You, Shin-Hyeong Cho, Won-Ja Lee
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Immune Responses of Mice Intraduodenally Infected withToxoplasma gondiiKI-1 Tachyzoites
Eun-Hee Shin, Yeoun Sook Chun, Won-Hee Kim, Jae-Lip Kim, Kyoung-Ho Pyo, Jong-Yil Chai
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Seroprevalence of Toxoplasma gondii Infection and Characteristics of Seropositive Patients in General Hospitals in Daejeon, Korea
Dae-Whan Shin, Dong-Yeub Cha, Quan Juan Hua, Guang-Ho Cha, Young-Ha Lee
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Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis
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Juan-Hua Quan, Tae Yun Kim, In-Uk Choi, Young-Ha Lee
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MIGRATION OF NEODIPLOSTOMUM LEEI (DIGENEA: NEODIPLOSTOMIDAE) NEODIPLOSTOMULA TO THE LIVERS OF VARIOUS MAMMALS
Eun-Hee Shin, Il-Myung Kim, Jae-Lip Kim, Eun-Taek Han, Yun-Kyu Park, Yukifumi Nawa, Jina Kook, Soon-Hyung Lee, Jong-Yil Chai
Journal of Parasitology.2006; 92(2): 223. CrossRef
Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii
Aifen Lin, Eun-Hee Shin, Tae-Yun Kim, Jae-Hwan Park, Sang-Mee Guk, Jong-Yil Chai
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Food-borne Parasitic Diseases
Jong-Yil Chai
Journal of the Korean Medical Association.2004; 47(6): 496. CrossRef

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Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda): Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong; Korean J Parasitol 2003;41(3):155-163.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.155

Abstract
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The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

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Protease activity of 80 kDa protein secreted from the apicomplexan parasite Toxoplasma gondii: Kyoung-Ju Song, Ho-Woo Nam; Korean J Parasitol 2003;41(3):165-169.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.165

Abstract
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This study describes the characterization of 80 kDa protease showing gelationlytic property among three proteases in the excretory/secretory proteins (ESP) from Toxoplasma gondii. The protease activity was detected in the ESP but not in the somatic extract of RH tachyzoites. This protease was active only in the presence of calcium ion but not other divalent cationic ions such as Cu²⁺, Zn²⁺, Mg²⁺, and Mn²⁺, implying that Ca²⁺ is critical factor for the activation of the protease. The 80 kDa protease was optimally active at pH 7.5. Its gelatinolytic activity was maximal at 37℃, and significant level of enzyme activity of the protease remained after heat treatment at 56℃ for 30 min or 100℃ for 10 min. This thermostable enzyme was strongly inhibited by metal chelators, i.e., EDTA, EGTA, and 1,10-phenanthroline. Thus, the 80 kDa protease n the ESP secreted by T. gondii was classified as a calcium dependent neutral metalloprotease.

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Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes
Amal Z. Barakat, Azza M. Abdel-Aty, Marwa K. Ibrahim, Hala A. Salah, Usama M. Hegazy, Rasha A. M. Azouz, Roqaya I. Bassuiny, Raafat M. Shaapan, Saleh A. Mohamed
Scientific Reports.2023;[Epub] CrossRef
In vitro activity of N-phenyl-1,10-phenanthroline-2-amines against tachyzoites and bradyzoites of Toxoplasma gondii
Erica S. Martins-Duarte, Juliana de Araujo Portes, Ramon Borges da Silva, Hugo Silva Pires, Simon J. Garden, Wanderley de Souza
Bioorganic & Medicinal Chemistry.2021; 50: 116467. CrossRef
Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells
Carlos J. Ramírez-Flores, Rosalba Cruz-Mirón, Rossana Arroyo, Mónica E. Mondragón-Castelán, Tais Nopal-Guerrero, Sirenia González-Pozos, Emmanuel Ríos-Castro, Ricardo Mondragón-Flores
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Case Report

A human case of house dust mite Tarsonemus floricolus collected from sputum: Jae-Sook Ryu, Han-Il Ree, Duk-Young Min, Myoung-Hee Ahn; Korean J Parasitol 2003;41(3):171-173.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.171

Abstract
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A 23-year-old medical student showed a positive reaction on a skin test for Paragonimus westermani, and two Tarsonemus floricolus mites were subsequently found by sputum examination and identified morphologically. Our report is the first human case of Tarsonemus floricolus in Korea.

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Prevalence and risk factors associated with intestinal acaroid mite and helminthic infection among school-aged children in Northern Egypt
Sarah M. Abdo, Mohamed Mostafa Tahoun, E. S. El-Wakil, Yusuf A. Edmardash, Hend A. El-Taweel, Heba Elhadad, Hend Hamdy, Ramy Mohamed Ghazy
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Brief Communication

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera: Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.175

Abstract
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An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

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