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Volume 49(3); September 2011

Original Articles

Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen
Chom-Kyu Chong, Wooseog Jeong, Hak-Yong Kim, Dong-Jun An, Hye-Young Jeoung, Jeong-Eun Ryu, A-Ra Ko, Yong-Joo Kim, Sung-Jong Hong, Zhaoshou Yang, Ho-Woo Nam
Korean J Parasitol 2011;49(3):207-212.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.207

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

Citations

Citations to this article as recorded by  Crossref logo
  • Development and optimization of an ELISA method to detect Toxoplasma gondii oocyst infection in cats
    Mingfeng He, Bufan Zhang, Shuai Han, Jiahui Qian, Zhengming He, Yulian Wei, Yanqin Zhou, Bang Shen, Rui Fang
    Parasitology Research.2025;[Epub]     CrossRef
  • Comprehensive diagnostic approaches to feline toxoplasmosis: Bridging traditional methods and emerging technologies
    Dan Zhao, Yanzhen Liao, Hao Liu, Jianwei Wang, Ruiying Liang, Rongqiong Zhou, Jiabo Ding, Sixin Zhang, Xinming Tang
    Virulence.2025;[Epub]     CrossRef
  • Evaluation of an immunochromatographic serologic test to detect the presence of anti‐Toxoplasma gondii antibodies in cats
    Sergio Villanueva‐Saz, Mariví Martínez, Jacobo Giner, María Dolores Pérez, Ana Pilar Tobajas, Andrés Yzuel, María Teresa Verde, Delia Lacasta, Antonio Fernández, Diana Marteles, Héctor Ruíz
    Veterinary Clinical Pathology.2023; 52(2): 284.     CrossRef
  • A preliminary study to develop a lateral flow assay using recombinant GRA1 protein for the diagnosis of toxoplasmosis in stray cats
    Aysu Değirmenci Döşkaya, Hüseyin Can, Aytül Gül, Tuğba Karakavuk, Mervenur Güvendi, Muhammet Karakavuk, Ceren Gül, Sedef Erkunt Alak, Cemal Ün, Adnan Yüksel Gürüz, Mert Döşkaya
    Comparative Immunology, Microbiology and Infectious Diseases.2023; 101: 102057.     CrossRef
  • Development and evaluation of indirect enzyme-linked immunosorbent assay using recombinant dense granule antigen 7 protein for the detection of Toxoplasma gondii infection in cats in Thailand
    Eukote Suwan, Piangjai Chalermwong, Rucksak Rucksaken, Metita Sussadee, Sarawan Kaewmongkol, Ruenruetai Udonsom, Sathaporn Jittapalapong, Bandid Mangkit
    Veterinary World.2022; : 602.     CrossRef
  • Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals
    Amjad Hayat Khan, Rahmah Noordin
    European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 19.     CrossRef
  • All about toxoplasmosis in cats: the last decade
    J.P. Dubey, C.K. Cerqueira-Cézar, F.H.A. Murata, O.C.H. Kwok, Y.R. Yang, C. Su
    Veterinary Parasitology.2020; 283: 109145.     CrossRef
  • Toxoplasma gondii Recombinant Antigens in the Serodiagnosis of Toxoplasmosis in Domestic and Farm Animals
    Bartłomiej Ferra, Lucyna Holec-Gąsior, Weronika Grąźlewska
    Animals.2020; 10(8): 1245.     CrossRef
  • Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens
    Benedikt T. Fabian, Fatima Hedar, Martin Koethe, Berit Bangoura, Pavlo Maksimov, Franz J. Conraths, Isabelle Villena, Dominique Aubert, Frank Seeber, Gereon Schares
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1
    Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam
    The Korean Journal of Parasitology.2019; 57(3): 283.     CrossRef
  • Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Chom-Kyu Chong, Yanaihara Pinchemel, Francis Reisdörfer, Joyce Brito Coelho, Ronaldo Ferreira Dias, Pan Kee Bae, Zuinara Pereira Maia Gusmão, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2018; 56(1): 61.     CrossRef
  • Disentangling the link between supplemental feeding, population density, and the prevalence of pathogens in urban stray cats
    Jusun Hwang, Nicole L. Gottdenker, Dae-Hyun Oh, Ho-Woo Nam, Hang Lee, Myung-Sun Chun
    PeerJ.2018; 6: e4988.     CrossRef
  • Spread and genotype of Toxoplasma gondii in naturally infected alpine chamois (Rupicapra r. rupicapra)
    Nicoletta Formenti, Alessandra Gaffuri, Tiziana Trogu, Roberto Viganò, Nicola Ferrari, Paolo Lanfranchi
    Parasitology Research.2016; 115(5): 2115.     CrossRef
  • Opportunities and challenges for the application of microfluidic technologies in point-of-care veterinary diagnostics
    Valentina Busin, Beth Wells, Maïwenn Kersaudy-Kerhoas, Wenmaio Shu, Stewart T.G. Burgess
    Molecular and Cellular Probes.2016; 30(5): 331.     CrossRef
  • Synthesis and characterization of latex-protein complexes from different antigens ofToxoplasma gondiifor immunoagglutination assays
    Leandro E. Peretti, Verónica D. G. Gonzalez, Juan G. Costa, Iván S. Marcipar, Luis M. Gugliotta
    International Journal of Polymeric Materials and Polymeric Biomaterials.2016; 65(18): 938.     CrossRef
  • Toxoplasma gondii Infection in Alpine Red Deer (Cervus elaphus): Its Spread and Effects on Fertility
    Nicoletta Formenti, Tiziana Trogu, Luca Pedrotti, Alessandra Gaffuri, Paolo Lanfranchi, Nicola Ferrari, Guido Favia
    PLOS ONE.2015; 10(9): e0138472.     CrossRef
  • Latex–protein complexes from an acute phase recombinant antigen of Toxoplasma gondii for the diagnosis of recently acquired toxoplasmosis
    Leandro E. Peretti, Verónica D.G. Gonzalez, Iván S. Marcipar, Luis M. Gugliotta
    Colloids and Surfaces B: Biointerfaces.2014; 120: 88.     CrossRef
  • A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
    Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
    The Korean Journal of Parasitology.2013; 51(5): 503.     CrossRef
  • Evaluation of an indirect ELISA using a tachyzoite surface antigen SAG1 for diagnosis of Toxoplasma gondii infection in cats
    M. Hosseininejad
    Experimental Parasitology.2012; 132(4): 556.     CrossRef
  • 10,550 View
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Changing Patterns of Acute Phase Proteins and Inflammatory Mediators in Experimental Caprine Coccidiosis
Mohammad Hashemnia, Azizollah Khodakaram-Tafti, Seyed Mostafa Razavi, Saeed Nazifi
Korean J Parasitol 2011;49(3):213-219.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.213

This experiment was conducted to assess the changing patterns and relative values of acute phase proteins and inflammatory cytokines in experimental caprine coccidiosis. Eighteen newborn kids were allocated to 3 equal groups. Two groups, A and B, were inoculated with a single dose of 1×103 and1×105 sporulated oocysts of Eimeria arloingi, respectively. The third group, C, received distilled water as the control. Blood samples were collected from the jugular vein of each kid in both groups before inoculation and at days 7, 14, 21, 28, 35, and 42 post-inoculation (PI), and the levels of haptoglobin (Hp), serum amyloid A (SAA), TNF-α, and IFN-γ were measured. For histopathological examinations, 2 kids were selected from each group, euthanized, and necropsied on day 42 PI. Mean Hp concentrations in groups A and B (0.34 and 0.68 g/L) at day 7 PI were 3.2 and 6.3 times higher than the levels before inoculation. The mean SAA concentrations in groups A and B (25.6 and 83.5 ?g/ml) at day 7 PI were 4.2 and 13.7 times higher than the levels before inoculation. The magnitude and duration of the Hp and SAA responses correlated well with the inoculation doses and the severity of the clinical signs and diarrhea in kids. These results were consistent with the histopathological features, which showed advanced widespread lesions in group B. In both groups, significant correlations were observed for TNF-α and IFN-γ with SAA and Hp, respectively. In conclusion, Hp and SAA can be useful non-specific diagnostic indicators in caprine coccidiosis.

Citations

Citations to this article as recorded by  Crossref logo
  • Global prevalence of Eimeria species in goats: a systematic review and meta-analysis
    Endris A. Ali, Abdul Ghafar, Juan C. Angeles-Hernandez, Muhammad Yaseen, Charles G. Gauci, Ian Beveridge, Sandra Baxendell, Abdul Jabbar
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Acute phase response and oxidative stress in coccidiosis: A review in domestic animals
    Seyed Mostafa Razavi, Mohammad Sajjad Soltan, Kiarash Abbasian, Ali Karami, Saeed Nazifi
    Veterinary Parasitology.2024; 331: 110286.     CrossRef
  • DEVELOPMENT OF A QUANTITATIVE IMMUNOASSAY FOR SERUM HAPTOGLOBIN AS A PUTATIVE DISEASE MARKER IN THE SOUTHERN WHITE RHINOCEROS (CERATOTHERIUM SIMUM SIMUM)
    Henrik H. Petersen, Rikke Stenbak, Camilla Blaabjerg, Anne K.H. Krogh, Mads F. Bertelsen, Peter Buss, Peter M.H. Heegaard
    Journal of Zoo and Wildlife Medicine.2022;[Epub]     CrossRef
  • Assessment of the Immune Response of Clinically Infected Calves to Cryptosporidium parvum Infection
    Wael El-Deeb, Olimpia Iacob, Mahmoud Fayez, Ibrahim Elsohaby, Abdulrahman Alhaider, Hermine V. Mkrtchyan, Abdelazim Ibrahim, Naser Alhumam
    Agriculture.2022; 12(8): 1151.     CrossRef
  • Comparative study on plasma fibrinogen and ceruloplasmin concentrations during pregnancy and postpartum period in Bulgarian native goats
    I. Fasulkov, M. Karadaev, D. Zapryanova, T. Mircheva, N. Vasilev, F. Ceciliani
    BULGARIAN JOURNAL OF VETERINARY MEDICINE.2022; 25(4): 603.     CrossRef
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    P.T. Iliev, T.M. Georgieva
    BULGARIAN JOURNAL OF VETERINARY MEDICINE.2019; 22(1): 1.     CrossRef
  • Haptoglobin and serum amyloid A in goats with clinical form of caprine arthritis-encephalitis
    Michał Czopowicz, Olga Szaluś-Jordanow, Marcin Mickiewicz, Agata Moroz, Lucjan Witkowski, Iwona Markowska-Daniel, Tadeusz Stefaniak, Emilia Bagnicka, Jarosław Kaba
    Small Ruminant Research.2017; 156: 73.     CrossRef
  • Agreement between commercial assays for haptoglobin and serum amyloid A in goats
    Michał Czopowicz, Olga Szaluś-Jordanow, Marcin Mickiewicz, Agata Moroz, Lucjan Witkowski, Iwona Markowska-Daniel, Daria Reczyńska, Emilia Bagnicka, Jarosław Kaba
    Acta Veterinaria Scandinavica.2017;[Epub]     CrossRef
  • Effect of Ketoprofen on acute phase protein concentrations in goats undergoing castration
    Umit Karademir, Ibrahim Akin, Hasan Erdogan, Kerem Ural, Gamze Sevri Ekren Asici
    BMC Veterinary Research.2016;[Epub]     CrossRef
  • Dynamic Patterns of Systemic Innate Immunity and Inflammatory Associated Factors in Experimental Caprine Coccidiosis
    Shabnam Tadayon, Seyed Mostafa Razavi, Saeed Nazifi
    The Korean Journal of Parasitology.2016; 54(6): 719.     CrossRef
  • Intestinal pathogens, diarrhoea and acute phase proteins in naturally infected dairy calves
    Leena Seppä-Lassila, Toomas Orro, Brian Lassen, Riikka Lasonen, Tiina Autio, Sinikka Pelkonen, Timo Soveri
    Comparative Immunology, Microbiology and Infectious Diseases.2015; 41: 10.     CrossRef
  • Systemic acute phase proteins response in calves experimentally infected with Eimeria zuernii
    Brian Lassen, Berit Bangoura, Triin Lepik, Toomas Orro
    Veterinary Parasitology.2015; 212(3-4): 140.     CrossRef
  • Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review
    C. Tothova, O. Nagy, G. Kovac
    Veterinární medicína.2014; 59(4): 163.     CrossRef
  • 10,218 View
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Expressed Sequence Tag Analysis of the Erythrocytic Stage of Plasmodium berghei
Ji-Woong Seok, Yong-Seok Lee, Eun-Kyung Moon, Jung-Yub Lee, Bijay Kumar Jha, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong
Korean J Parasitol 2011;49(3):221-228.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.221

Rodent malaria parasites, such as Plasmodium berghei, are practical and useful model organisms for human malaria research because of their analogies to the human malaria in terms of structure, physiology, and life cycle. Exploiting the available genetic sequence information, we constructed a cDNA library from the erythrocytic stages of P. berghei and analyzed the expressed sequence tag (EST). A total of 10,040 ESTs were generated and assembled into 2,462 clusters. These EST clusters were compared against public protein databases and 48 putative new transcripts, most of which were hypothetical proteins with unknown function, were identified. Genes encoding ribosomal or membrane proteins and purine nucleotide phosphorylases were highly abundant clusters in P. berghei. Protein domain analyses and the Gene Ontology functional categorization revealed translation/protein folding, metabolism, protein degradation, and multiple family of variant antigens to be mainly prevalent. The presently-collected ESTs and its bioinformatic analysis will be useful resources to identify for drug target and vaccine candidates and validate gene predictions of P. berghei.

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Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study
Hee Jung Choi, Jee Youn Hwang, Dong Lim Choi, Min Do Huh, Young Baek Hur, Nam-Sil Lee, Jung Soo Seo, Mun Gyeong Kwon, Hye-Sung Choi, Myoung Ae Park
Korean J Parasitol 2011;49(3):229-234.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.229

In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.

Citations

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  • Transcriptome-wide identification, functional characterization, and expression analysis of two novel invertebrate-type Toll-like receptors from disk abalone (Haliotis discus discus)
    Thanthrige Thiunuwan Priyathilaka, S.D.N.K. Bathige, Seongdo Lee, Bo-Hye Nam, Jehee Lee
    Fish & Shellfish Immunology.2019; 84: 802.     CrossRef
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    IOP Conference Series: Earth and Environmental Science.2019; 236: 012022.     CrossRef
  • The role of copper and zinc accumulation in defense against bacterial pathogen in the fujian oyster (Crassostrea angulata)
    Bo Shi, Tian Wang, Zhen Zeng, Long Zhou, Weiwei You, Caihuan Ke
    Fish & Shellfish Immunology.2019; 92: 72.     CrossRef
  • Hybridization improved bacteria resistance in abalone: Evidence from physiological and molecular responses
    Shuang Liang, Xuan Luo, Weiwei You, Caihuan Ke
    Fish & Shellfish Immunology.2018; 72: 679.     CrossRef
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    A. A. Anisimova
    Russian Journal of Marine Biology.2013; 39(6): 381.     CrossRef
  • Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)
    Fernando Ramos Queiroga, Luis Fernando Marques-Santos, Hélène Hégaret, Philippe Soudant, Natanael Dantas Farias, Aline Daiane Schlindwein, Patricia Mirella da Silva
    Fish & Shellfish Immunology.2013; 35(2): 319.     CrossRef
  • Relative contribution of reproductive investment, thermal stress and Vibrio infection to summer mortality phenomena in Pacific oysters
    Carolin C. Wendling, K. Mathias Wegner
    Aquaculture.2013; 412-413: 88.     CrossRef
  • Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity
    Yang Zhang, Xiaocui He, Feng Yu, Zhiming Xiang, Jun Li, Karen L. Thorpe, Ziniu Yu, Irving Coy Allen
    PLoS ONE.2013; 8(10): e76464.     CrossRef
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  • 89 Download
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Protease-Activated Receptor 2 Is Involved in Th2 Responses against Trichinella spiralis Infection
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu
Korean J Parasitol 2011;49(3):235-243.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.235

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.

Citations

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  • A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway
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    Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Sang Kyun Park, Min Seong Jang, Bo-Gie Yang, Myoung Ho Jang, Dong-Hee Kim, Hak Sun Yu, Edward Mitre
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    Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu
    The Korean Journal of Parasitology.2013; 51(5): 583.     CrossRef
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    Ivan Zanoni, Francesca Granucci
    European Journal of Immunology.2012; 42(8): 1924.     CrossRef
  • TSLP Expression: Cellular Sources, Triggers, and Regulatory Mechanisms
    Toshiro Takai
    Allergology International.2012; 61(1): 3.     CrossRef
  • Regulation of epithelial immunity by IL-17 family cytokines
    Rajita Pappu, Sascha Rutz, Wenjun Ouyang
    Trends in Immunology.2012; 33(7): 343.     CrossRef
  • Regulation of type 2 immunity to helminths by mast cells
    Matthew R. Hepworth, Marcus Maurer, Susanne Hartmann
    Gut Microbes.2012; 3(5): 476.     CrossRef
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Parasitic Helminth Cystatin Inhibits DSS-Induced Intestinal Inflammation Via IL-10+F4/80+ Macrophage Recruitment
Sung Won Jang, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Byoung-Kuk Na, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu
Korean J Parasitol 2011;49(3):245-254.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.245

Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-γ and TNF-α in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-α in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10+F4/80+ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages.

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In Vitro Effects of Some Herbs Used in Egyptian Traditional Medicine on Viability of Protoscolices of Hydatid Cysts
Doaa A. Yones, Gamal A. Taher, Zedan Z. Ibraheim
Korean J Parasitol 2011;49(3):255-263.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.255

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 ?g/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 ?g/ml) were used. Concentration of 2,500 ?g/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 ?g/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 ?g/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 ?g/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

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  • In vitro and in vivo antihydatid activity of a nano emulsion of Zataria multiflora essential oil
    Mohammad Moazeni, Hassan Borji, Meysam Saboor Darbandi, Mohammad Jamal Saharkhiz
    Research in Veterinary Science.2017; 114: 308.     CrossRef
  • In vitro effects of Blepharocalyx salicifolius (H.B.K.) O. Berg on the viability of Echinococcus ortleppi protoscoleces
    Charlise Bolson Noal, Danieli Urach Monteiro, Thiele Faccim de Brum, Jessica Emmanouilidis, Regis Adriel Zanette, Ademir Farias Morel, Eliza Beti de Cassia Stefanon, Marina Frosi, Mario Luiz de la Rue
    Revista do Instituto de Medicina Tropical de São Paulo.2017;[Epub]     CrossRef
  • Natural products applied against hydatid cyst protoscolices: A review of past to present
    Mohammad Hasan Kohansal, Abbasali Nourian, Mohammad Taghi Rahimi, Ahmad Daryani, Adel Spotin, Ehsan Ahmadpour
    Acta Tropica.2017; 176: 385.     CrossRef
  • Antihydatic and immunomodulatory effects of Punica granatum peel aqueous extract in a murine model of echinococcosis
    Moussa Labsi, Lila Khelifi, Dalila Mezioug, Imene Soufli, Chafia Touil-Boukoffa
    Asian Pacific Journal of Tropical Medicine.2016; 9(3): 211.     CrossRef
  • Evaluation of the Hydatid Cyst Membrane Permeability of Albendazole and Albendazole Sulfoxide-Loaded Solid Lipid Nanoparticles
    Shahrzad Soltani, Abdollah Rafiei, Zahra Ramezani, Mohammad Reza Abbaspour, Ali Jelowdar, Mehdi Sagha Kahvaz
    Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub]     CrossRef
  • Protoscolicidal and immunomodulatory activity of Ziziphora tenuior extract and its fractions
    Mojtaba Shahnazi, Abbas Azadmehr, Ammar Andalibian, Reza Hajiaghaee, Mehrzad Saraei, Mahmood Alipour
    Asian Pacific Journal of Tropical Medicine.2016; 9(11): 1062.     CrossRef
  • Evaluation of the Hydatid Cyst Membrane Permeability of Albendazole and Albendazole Sulfoxide-Loaded Solid Lipid Nanoparticles
    Shahrzad Soltani, Abdollah Rafiei, Zahra Ramezani, Mohammad Reza Abbaspour, Ali Jelowdar, Mehdi Sagha Kahvaz
    Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub]     CrossRef
  • Osmoregulatory and tegumental ultrastructural damages to protoscoleces of hydatid cysts Echinococcus granulosus induced by fungal endophytes
    Vijay C. Verma, Mayank Gangwar, Gopal Nath
    Journal of Parasitic Diseases.2014; 38(4): 432.     CrossRef
  • Anticestodal Activity of EndophyticPestalotiopsissp. on Protoscoleces of Hydatid CystEchinococcus granulosus
    Vijay C. Verma, Mayank Gangwar, Madhu Yashpal, Gopal Nath
    BioMed Research International.2013; 2013: 1.     CrossRef
  • In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus
    Mayank Gangwar, Vijay C Verma, Tryambak D Singh, Sushil K Singh, RK Goel, Gopal Nath
    Asian Pacific Journal of Tropical Medicine.2013; 6(8): 595.     CrossRef
  • 14,440 View
  • 201 Download
  • Crossref

Case Reports

Two Cases of Human Thelaziasis and Brief Review of Korean cases
Woon-Mok Sohn, Byoung-Kuk Na, Ji Myong Yoo
Korean J Parasitol 2011;49(3):265-271.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.265

The present study was performed to describe 2 cases of human thelaziasis (HT) which occurred in Gyeongsangnam- do and to briefly review the previously reported Korean cases. A 58-year old woman, residing in Hadong-gun, Gyeongsangnam-do, came to Gyeongsang National University Hospital (GNUH) complaining of foreign body sensation and itching of the right eye in March 2000. Total 6 adult nematodes of Thelazia callipaeda (2 males and 4 females) were detected in her right eye. A 80-year old man, residing in Jinju-si, Gyeongsangnam-do, came to GNUH complaining of foreign body sensation, itching, and pain of the right eye in December 2007. A total of 5 worms (4 females and 1 degenerated) were removed from his right eye. We analyzed characteristics of the total 39 Korean HT cases reported to date, including the present 2 cases. Most of the cases (71.8%) occurred in Seoul and Gyeonggi-do before 2000, and 21 cases (53.8%) were males and 18 (46.2%) were females. The prevalence was higher in younger ages below 30 years (48.7%) than 31-60 years (41.0%) and over 61 years (10.3%). The seasonal prevalence showed a higher incidence in autumn (43.6%) than in other seasons. Most of the cases (94.9%) were conjunctival sac infections and only 2 (5.1%) were intraocular cases. The present 2 HT cases are the first reported cases in Gyeongsangnam-do. Some characteristics of Korean HT cases were analyzed.

Citations

Citations to this article as recorded by  Crossref logo
  • Thelazia callipaeda (Nematoda: Spirurida) infections in 2 wildlife species, Prionailurus bengalensis and Nyctereutes procyonoides, in Korea
    Jinho Jang, Young-Seok Park, YoungMin Yun, Keeseon S. Eom, Seongjun Choe
    Parasites, Hosts and Diseases.2025; 63(1): 95.     CrossRef
  • Thelaziasis in humans: A systematic review of reported cases
    D. Katterine Bonilla-Aldana, Jorge Luis Bonilla-Aldana, Jaime David Acosta-España, Ranjit Sah, Alfonso J. Rodriguez-Morales
    New Microbes and New Infections.2025; 65: 101599.     CrossRef
  • Eight cases of canine thelaziosis found in two localities in Korea
    Seongjun Choe, Sunmin Kim, Tilak Chandra Nath, Jeong-Ho Kim
    Parasites, Hosts and Diseases.2023; 61(3): 325.     CrossRef
  • A Case of Human Thelaziasis and Review of Chinese Cases
    Shi Nan Liu, Fang Fang Xu, Wen Qing Chen, Peng Jiang, Jing Cui, Zhong Quan Wang, Xi Zhang
    Acta Parasitologica.2020; 65(3): 783.     CrossRef
  • A human corneal ulcer caused by Thelazia callipaeda in Southwest China: case report
    Xiaoxing Wei, Bo Liu, Yijian Li, Ke Wang, Lixia Gao, Yuli Yang
    Parasitology Research.2020; 119(10): 3531.     CrossRef
  • Periocular thelaziasis presenting as an orbital mass – a case report
    Kirthi Koka, Andrea Tongbram, Bipasha Mukherjee, Raman Muthusamy, Azhahia Nambi, Jyotirmay Biswas
    Orbit.2019; 38(6): 503.     CrossRef
  • A Pediatric Case of Thelaziasis in Korea
    Chung Hyuk Yim, Jeong Hee Ko, Jung Hyun Lee, Yu Mi Choi, Won Wook Lee, Sang Ki Ahn, Myoung Hee Ahn, Kyong Eun Choi
    The Korean Journal of Parasitology.2016; 54(3): 319.     CrossRef
  • Ocular thelaziasis in a 7-month-old infant
    AK Handique, A Tamuli, AM Khan
    Indian Journal of Medical Microbiology.2014; 32(1): 84.     CrossRef
  • Mitochondrial Genome of the Eyeworm, Thelazia callipaeda (Nematoda: Spirurida), as the First Representative from the Family Thelaziidae
    Guo-Hua Liu, Robin B. Gasser, Domenico Otranto, Min-Jun Xu, Ji-Long Shen, Namitha Mohandas, Dong-Hui Zhou, Xing-Quan Zhu, Peter U. Fischer
    PLoS Neglected Tropical Diseases.2013; 7(1): e2029.     CrossRef
  • The First Human Case of Thelazia callipaeda Infection in Vietnam
    Nguyen Van De, Thanh Hoa Le, Jong-Yil Chai
    The Korean Journal of Parasitology.2012; 50(3): 221.     CrossRef
  • 9,548 View
  • 80 Download
  • Crossref
Hypersensitive Reaction to Praziquantel in a Clonorchiasis Patient
Jung-Min Lee, Hyun-Sul Lim, Sung-Tae Hong
Korean J Parasitol 2011;49(3):273-275.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.273

Praziquantel is the drug of choice for clonorchiasis. Since clonorchiasis is endemic in most river basins, praziquantel has been widely used for 30 years in Korea. A 54-year-old Korean woman suffered from hypersensitive reactions, such as nausea, dyspnea, rash, and urticaria after taking the first dose of praziquantel to treat clonorchiasis. She ingested one dose again and the same symptoms appeared, and she was treated at a clinic with anti-histamines. She tried one more dose with anti-histamines but found the same symptoms. Later, she was found to pass eggs of Clonorchis sinensis and medicated with flubendazole. The hypersensitive reaction to praziquantel is rare but occurs. This is the 5th case report in the world.

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  • Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control
    Men-Bao Qian, Jennifer Keiser, Jürg Utzinger, Xiao-Nong Zhou, Graeme N. Forrest
    Clinical Microbiology Reviews.2024;[Epub]     CrossRef
  • Clonorchis sinensis aggravated liver fibrosis by activating PARP-1 signaling to induce parthanatos via DNA damage
    Penglin Bao, Xiaocen Wang, Xu Zhang, Yanhui Yu, Yeting Ma, Haoyang Zhang, Yuru Wang, Xiaolei Liu, Pengtao Gong, Nan Zhang, Soon-Ok Lee, Xin Li, Jianhua Li
    Veterinary Parasitology.2024; 330: 110217.     CrossRef
  • Anaphylactic reaction to praziquantel following schistosomiasis treatment
    Géssica Almeida Vasconcelos, Bernardo Gratival Gouvea Costa, Ronald Alves dos Santos, Carolina Dourado de Faria, Fernando Antônio Ramos Schramm Neto, Yuri de Jesus Machado, Ane Caroline Casaes, Marcos Vinicius Lima de Oliveira, Thainá Rodrigues de Souza F
    IJID Regions.2023; 7: 268.     CrossRef
  • Development of acute pancreatitis after oral administering a praziquantel, pyrantel pamoate, and febantel combination in a dog: A case report
    Masashi Yuki, Hiroto Taira, Takanori Inden
    Heliyon.2023; 9(6): e17225.     CrossRef
  • Functional characterization of Clonorchis sinensis choline transporter
    Jeong Yeon Won, Johnsy Mary Louis, Eui Sun Roh, Seok Ho Cha, Jin-Hee Han
    Parasites, Hosts and Diseases.2023; 61(4): 428.     CrossRef
  • Identification and characterization of sirtuin enzymes in cestodes and evaluation of sirtuin inhibitors as new cestocidal molecules
    Hugo Rolando Vaca, Ana María Celentano, María Agustina Toscanini, Alexander-Thomas Hauser, Natalia Macchiaroli, María Luján Cuestas, Alejandro David Nusblat, Wolfgang Sippl, María Celina Elissondo, Manfred Jung, Federico Camicia, Mara Cecilia Rosenzvit
    International Journal for Parasitology.2022; 52(5): 317.     CrossRef
  • Molecular characterization of the serotonergic transporter from the cestode Echinococcus granulosus: pharmacology and potential role in the nervous system
    Federico Camicia, Hugo R. Vaca, Ines Guarnaschelli, Uriel Koziol, Ole V. Mortensen, Andreia C. K. Fontana
    Parasitology Research.2022; 121(5): 1329.     CrossRef
  • The potential for histone deacetylase (HDAC) inhibitors as cestocidal drugs
    Hugo Rolando Vaca, Ana María Celentano, María Agustina Toscanini, Tino Heimburg, Ehab Ghazy, Patrik Zeyen, Alexander-Thomas Hauser, Guilherme Oliveira, María Celina Elissondo, Manfred Jung, Wolfgang Sippl, Federico Camicia, Mara Cecilia Rosenzvit, Robin B
    PLOS Neglected Tropical Diseases.2021; 15(3): e0009226.     CrossRef
  • Novel mechanism of hepatobiliary system damage and immunoglobulin G4 elevation caused by Clonorchis sinensis infection
    Xin-He Zhang, Die Huang, Yi-Ling Li, Bing Chang
    World Journal of Clinical Cases.2021; 9(23): 6639.     CrossRef
  • Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis
    Shan Li, Xueqing Chen, Juanjuan Zhou, Zhizhi Xie, Mei Shang, Lei He, Pei Liang, Tingjin Chen, Qiang Mao, Chi Liang, Xuerong Li, Yan Huang, Xinbing Yu, John Pius Dalton
    PLOS Neglected Tropical Diseases.2020; 14(4): e0008287.     CrossRef
  • Practical Guidance for the Evaluation and Management of Drug Hypersensitivity: Specific Drugs
    Ana Dioun Broyles, Aleena Banerji, Sara Barmettler, Catherine M. Biggs, Kimberly Blumenthal, Patrick J. Brennan, Rebecca G. Breslow, Knut Brockow, Kathleen M. Buchheit, Katherine N. Cahill, Josefina Cernadas, Anca Mirela Chiriac, Elena Crestani, Pascal De
    The Journal of Allergy and Clinical Immunology: In Practice.2020; 8(9): S16.     CrossRef
  • Evaluation of anthelminthic activity of both pomegranate peels and Artemisia herba-Alba extracts in comparison with praziquantel in experimentally infected mice with Hymenolepis nana
    AzzaH Abbas, FetouhS Hassanin, NeveenM Sarhan, HebaM Abdelgalil
    Kasr Al Ainy Medical Journal.2020; 26(2): 76.     CrossRef
  • Histone deacetylase enzymes as potential drug targets of Neglected Tropical Diseases caused by cestodes
    Hugo R. Vaca, Ana M. Celentano, Natalia Macchiaroli, Laura Kamenetzky, Federico Camicia, Mara C. Rosenzvit
    International Journal for Parasitology: Drugs and Drug Resistance.2019; 9: 120.     CrossRef
  • Case Report: Acute Generalized Exanthematous Pustulosis Caused by Praziquantel
    Tatsuya Shindo, Yoshiyuki Masuda, Yukihiro Imai, Tohru Nagano, Hiroaki Nishioka
    The American Journal of Tropical Medicine and Hygiene.2019; 100(3): 700.     CrossRef
  • Albendazole and Praziquantel: Review and Safety Monitoring in Korea
    Sung-Tae Hong
    Infection & Chemotherapy.2018; 50(1): 1.     CrossRef
  • Photodynamic therapy for Schistosoma mansoni: Promising outcomes
    Nathália Bandeira de Melo, Letícia Fernanda Moreira dos Santos, Mayara Santos de Castro, Raquel Lopes Martins Souza, Marcos José Marques, Aline Pereira Castro, Andreísa Teixeira de Castro, Marina Lara de Carli, João Adolfo Costa Hanemann, Matheus Siqueira
    Journal of Photochemistry and Photobiology B: Biology.2017; 176: 157.     CrossRef
  • Clonorchiasis
    Men-Bao Qian, Jürg Utzinger, Jennifer Keiser, Xiao-Nong Zhou
    The Lancet.2016; 387(10020): 800.     CrossRef
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    Ze-Li Tang, Yan Huang, Xin-Bing Yu
    Infectious Diseases of Poverty.2016;[Epub]     CrossRef
  • Treatment of Schistosomiasis in a Patient Allergic to Praziquantel: A Desensitization and Treatment Protocol
    Trupti A. Patel, Robin L. Bailey, Joanna Lukawska, Jennifer Rowe
    The American Journal of Tropical Medicine and Hygiene.2016; 95(5): 1041.     CrossRef
  • Praziquantel Treatment in Trematode and Cestode Infections: An Update
    Jong-Yil Chai
    Infection & Chemotherapy.2013; 45(1): 32.     CrossRef
  • An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis
    Eun Joo Chung, Tae Yun Kim, Sung-Jong Hong, Tai-Soon Yong
    Parasitology Research.2013; 112(12): 4121.     CrossRef
  • 9,294 View
  • 90 Download
  • Crossref
Cystic Echinococcosis of the Pelvic Bone with Recurrences: A Case Report
Sunila Jain, Prem Chopra
Korean J Parasitol 2011;49(3):277-279.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.277

Hydatid cysts commonly affect the liver and the lung. However, they rarely involve bones with vertebral column. We hereby report a case of a female patient with cystic echinococcosis of the hip bone and ilium. She presented with a long history of frequent recurrences highlighting the dismal prognosis at this rare site. Resection of the hydatid cyst from the sacroiliac region was done with allograft and autograft (rib graft) with lumbosacroiliac fixation. Follow-up of the patient at 6 months showed no detectable abnormality on radiology and the patient was doing well.

Citations

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  • Intramuscular Hydatid Cyst: A Literature Review on Clinical Findings, Diagnosis, and Treatment
    Furkan Erdoğan, Tolgahan Cengiz, Şafak Aydın Şimşek, Levent Şensoy, Sancar  Barış, Nevzat Dabak
    Ahi Evran Medical Journal.2025; 9(2): 267.     CrossRef
  • Segmental Defect-Bridging Intramedullary Knee Arthrodesis for Osseous Hydatidosis of the Distal Femur: A Case Report
    Enejd Veizi, Ahmet Fırat, Şahin Çepni, Hacı M İnan, Kasım Kılıçarslan
    Cureus.2021;[Epub]     CrossRef
  • Cystic Echinococcosis of the Ilium Treated with Curettage and Microwave Thermoablation Followed by Bone Cement Installation: A Novel Treatment Technique for a Rare Disease
    Kyriakos Papavasiliou, Sousana Panagiotidou, Panagiotis Kakoulidis, Antonia Bintoudi, Kostoula Arvaniti, Eleftherios Tsiridis, Mark K. Lyons
    Case Reports in Orthopedics.2021; 2021: 1.     CrossRef
  • Loosening of a Total Hip Arthroplasty Associated with a Periprosthetic Femoral Fracture Caused by Recurrent Hydatidosis of the Hip
    Karim Masmoudi, Mahmoud Ben Maitigue, Riadh Frikha, Thabet Mouelhi, Hassen Cheikhrouhou
    JBJS Case Connector.2019; 9(4): e0044.     CrossRef
  • Combination drug chemotherapy and massive skeletal allograft in the management of hydatid disease of femur
    Deepak Gautam, Rajesh Malhotra, Siddharth Dubey
    BMJ Case Reports.2018; 2018: bcr-2017-223332.     CrossRef
  • Pathological fracture of femoral neck in a middle-aged woman: a rare presentation of primary hydatid cyst disease in humans
    FNU Salman, Muhammad Ihtesham Khan, Ishtiaq Hussain, Hafez Mohammad Ammar Abdullah
    BMJ Case Reports.2018; 2018: bcr-2017-222980.     CrossRef
  • Hydatid Disease of the Femur Treated with a Total Femoral Replacement
    Drew Moore, Kevin C. Baker, Kimberly Les
    JBJS Case Connector.2015; 5(1): e7.     CrossRef
  • Treatment challenges associated with bone echinococcosis
    S. Steinmetz, G. Racloz, R. Stern, D. Dominguez, M. Al-Mayahi, M. Schibler, D. Lew, P. Hoffmeyer, I. Uckay
    Journal of Antimicrobial Chemotherapy.2014; 69(3): 821.     CrossRef
  • 9,553 View
  • 75 Download
  • Crossref
Brief Communications
PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
Joung-Ho Moon, Shin-Hyeong Cho, Jae-Ran Yu, Won-Ja Lee, Hyeng-Il Cheun
Korean J Parasitol 2011;49(3):281-284.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.281

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.

Citations

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  • Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal Tract
    Lynne S. Garcia, Michael Arrowood, Evelyne Kokoskin, Graeme P. Paltridge, Dylan R. Pillai, Gary W. Procop, Norbert Ryan, Robyn Y. Shimizu, Govinda Visvesvara
    Clinical Microbiology Reviews.2018;[Epub]     CrossRef
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    Chao Lv, Yang Hong, Zhiqiang Fu, Ke Lu, Xiaodan Cao, Tao Wang, Chuangang Zhu, Hao Li, Rui Xu, Bingguang Jia, Qian Han, Xuefeng Dou, Yuanxi Shen, Zuhang Zhang, Jinli Zai, Jintao Feng, Jiaojiao Lin
    Parasites & Vectors.2016;[Epub]     CrossRef
  • Frequency of amoebiasis and other intestinal parasitoses in a settlement in Ilhéus City, State of Bahia, Brazil
    Helena Lúcia Carneiro Santos, Luci Ana Fernandes Martins, Regina Helena Saramago Peralta, José Mauro Peralta, Heloisa Werneck de Macedo
    Revista da Sociedade Brasileira de Medicina Tropical.2014; 47(1): 101.     CrossRef
  • 9,659 View
  • 101 Download
  • Crossref
Naegleria fowleri Lysate Induces Strong Cytopathic Effects and Pro-inflammatory Cytokine Release in Rat Microglial Cells
Yang-Jin Lee, Chang-Eun Park, Jong-Hyun Kim, Hae-Jin Sohn, Jinyoung Lee, Suk-Yul Jung, Ho-Joon Shin
Korean J Parasitol 2011;49(3):285-290.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.285

Naegleria fowleri, a ubiquitous free-living ameba, causes fatal primary amebic meningoencephalitis in humans. N. fowleri trophozoites are known to induce cytopathic changes upon contact with microglial cells, including necrotic and apoptotic cell death and pro-inflammatory cytokine release. In this study, we treated rat microglial cells with amebic lysate to probe contact-independent mechanisms for cytotoxicity, determining through a combination of light microscopy and scanning and transmission electron microscopy whether N. fowleri lysate could effect on both necrosis and apoptosis on microglia in a time- as well as dose-dependent fashion. A 51Cr release assay demonstrated pronounced lysate induction of cytotoxicity (71.5%) toward microglial cells by 24 hr after its addition to cultures. In an assay of pro-inflammatory cytokine release, microglial cells treated with N. fowleri lysate produced TNF-α, IL-6, and IL-1β, though generation of the former 2 cytokines was reduced with time, and that of the last increased throughout the experimental period. In summary, N. fowleri lysate exerted strong cytopathic effects on microglial cells, and elicited pro-inflammatory cytokine release as a primary immune response.

Citations

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  • Providing an in vitro depiction of microglial cells challenged with immunostimulatory extracellular vesicles of Naegleria fowleri
    Lissette Retana Moreira, Alberto Cornet-Gomez, M. Rosario Sepulveda, Silvia Molina-Castro, Johan Alvarado-Ocampo, Frida Chaves Monge, Mariana Jara Rojas, Antonio Osuna, Elizabeth Abrahams Sandí
    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • Transcriptomic profiling of “brain-eating amoeba” Naegleria fowleri infection in mice: the host and the protozoa perspectives
    Vincent Guerlais, Nina Allouch, E. Ashley Moseman, Alicja W. Wojciechowska, Jakub W. Wojciechowski, Isabel Marcelino
    Frontiers in Cellular and Infection Microbiology.2024;[Epub]     CrossRef
  • Pro-inflammatory cytokine responses to Naegleria fowleri infection
    Ching-wen Chen, E. Ashley Moseman
    Frontiers in Tropical Diseases.2023;[Epub]     CrossRef
  • Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells
    Hương Giang Lê, Jung-Mi Kang, Tuấn Cường Võ, Won Gi Yoo, Byoung-Kuk Na
    International Journal of Molecular Sciences.2023; 24(17): 13623.     CrossRef
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    Maarten J. Sarink, Nadia L. van der Meijs, Kristin Denzer, Leo Koenderman, Aloysius G.M. Tielens, Jaap J. van Hellemond
    Trends in Parasitology.2022; 38(3): 230.     CrossRef
  • Extracellular Vesicles from Naegleria fowleri Induce IL-8 Response in THP-1 Macrophage
    Sakaorat Lertjuthaporn, Jinjuta Somkird, Kittima Lekmanee, Anyapat Atipimonpat, Kasama Sukapirom, Hathai Sawasdipokin, Supathra Tiewcharoen, Kovit Pattanapanyasat, Ladawan Khowawisetsut
    Pathogens.2022; 11(6): 632.     CrossRef
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    Hương Giang Lê, Jung-Mi Kang, Tuấn Cường Võ, Byoung-Kuk Na
    International Journal of Molecular Sciences.2022; 23(15): 8388.     CrossRef
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    Jérémy Alloo, Ines Leleu, Corinne Grangette, Sylviane Pied
    Frontiers in Immunology.2022;[Epub]     CrossRef
  • Fowlerstefin, a cysteine protease inhibitor of Naegleria fowleri, induces inflammatory responses in BV-2 microglial cells in vitro
    Thị Lam Thái, Jung-Mi Kang, Hương Giang Lê, Jinyoung Lee, Won Gi Yoo, Ho-Joon Shin, Woon-Mok Sohn, Byoung-Kuk Na
    Parasites & Vectors.2020;[Epub]     CrossRef
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    Moisés Martínez-Castillo, Leopoldo Santos-Argumedo, José Manuel Galván-Moroyoqui, Jesús Serrano-Luna, Mineko Shibayama
    Parasitology Research.2018; 117(1): 75.     CrossRef
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    Xing Wu, Tengbo Yu, Hongyan Xu, Xiuming Sun, Dewei Kou, Liping Li
    Experimental and Therapeutic Medicine.2017;[Epub]     CrossRef
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    Ruqaiyyah Siddiqui, Ibne Karim M. Ali, Jennifer R. Cope, Naveed Ahmed Khan
    Acta Tropica.2016; 164: 375.     CrossRef
  • NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
    Jong-Hyun Kim, Hae-Jin Sohn, Jong-Kyun Yoo, Heekyoung Kang, Gi-Sang Seong, Yong-Joon Chwae, Kyongmin Kim, Sun Park, Ho-Joon Shin, J. A. Appleton
    Infection and Immunity.2016; 84(9): 2422.     CrossRef
  • Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory–secretory proteins and their biochemical properties
    Jinyoung Lee, Jong-Hyun Kim, Hae-Jin Sohn, Hee-Jong Yang, Byoung-Kuk Na, Yong-Joon Chwae, Sun Park, Kyongmin Kim, Ho-Joon Shin
    Parasitology Research.2014; 113(8): 2765.     CrossRef
  • 10,811 View
  • 103 Download
  • Crossref
NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products
Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
Korean J Parasitol 2011;49(3):291-294.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.291

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1×107 live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-κB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with iκB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-κB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

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  • Trichomonas vaginalis extracellular vesicles suppress IFNε-mediated responses driven by its intracellular bacterial symbiont Mycoplasma hominis
    Joshua A. Kochanowsky, Emma L. Betts, Gabriel Encinas, Johnson Amoah, Patricia J. Johnson
    Proceedings of the National Academy of Sciences.2025;[Epub]     CrossRef
  • CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
    Young Ah Lee, Myeong Heon Shin
    Parasites, Hosts and Diseases.2024; 62(3): 270.     CrossRef
  • Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
    Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
    Acta Tropica.2023; 238: 106794.     CrossRef
  • Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation
    Shao-Chun Wu, Pao-Jen Kuo, Cheng-Shyuan Rau, Lien-Hung Huang, Chia-Wei Lin, Yi-Chan Wu, Chia-Jung Wu, Chia-Wen Tsai, Ting-Min Hsieh, Hang-Tsung Liu, Chun-Ying Huang, Ching-Hua Hsieh
    International Journal of Molecular Sciences.2021; 22(16): 8877.     CrossRef
  • Trichomonas vaginalis‐secreted cysteinyl leukotrienes promote migration, degranulation and MCP‐1 production in mast cells
    Young Ah Lee, Young Hee Nam, Arim Min, Myeong Heon Shin
    Parasite Immunology.2020;[Epub]     CrossRef
  • PKA/CREB and NF-κB pathway regulates AKNA transcription: A novel insight into T-2 toxin-induced inflammation and GH deficiency in GH3 cells
    Xianglian Liu, Deyu Huang, Pu Guo, Qinghua Wu, Menghong Dai, Guyue Cheng, Haihong Hao, Shuyu Xie, Zonghui Yuan, Xu Wang
    Toxicology.2017; 392: 81.     CrossRef
  • SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete
    Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton
    Infection and Immunity.2017;[Epub]     CrossRef
  • Influence of functional polymorphisms in TNF-α, IL-8, and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer
    Juliana Garcia de Oliveira, Ana Flávia Teixeira Rossi, Daniela Manchini Nizato, Aline Cristina Targa Cadamuro, Yvana Cristina Jorge, Marina Curado Valsechi, Larissa Paola Rodrigues Venâncio, Paula Rahal, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo,
    Tumor Biology.2015; 36(12): 9159.     CrossRef
  • The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis
    Wei-Chen Lin, Wei-Ting Chang, Tsuey-Yu Chang, Jyh-Wei Shin, Xuefeng Liu
    PLOS ONE.2015; 10(4): e0124087.     CrossRef
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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
Woojin Pi, Jae-Sook Ryu, Jaesook Roh
Korean J Parasitol 2011;49(3):295-298.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.295

Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.

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  • Effect of Pediococcus acidilactici and mango seed polyphenols on the fermentative profile of the indigestible fraction of yam bean
    Alba Cecilia Durán-Castañeda, Adela Yolanda Bueno-Durán, Manuel Iván Girón-Pérez, Juan Arturo Ragazzo-Sánchez, Jorge Alberto Sánchez-Burgos, Sonia Guadalupe Sáyago-Ayerdi, Victor Manuel Zamora-Gasga
    Food Research International.2024; 178: 113970.     CrossRef
  • In Vitro Digestion of Vacuum-Impregnated Yam Bean Snacks: Pediococcus acidilactici Viability and Mango Seed Polyphenol Bioaccessibility
    Alba Cecilia Durán-Castañeda, Adela Yolanda Bueno-Durán, Manuel Iván Girón-Pérez, Juan Arturo Ragazzo-Sánchez, Jorge Alberto Sánchez-Burgos, Sonia Guadalupe Sáyago-Ayerdi, Victor Manuel Zamora-Gasga
    Microorganisms.2024; 12(10): 1993.     CrossRef
  • Safety Assessment and Probiotic Potential Comparison of Bifidobacterium longum subsp. infantis BLI-02, Lactobacillus plantarum LPL28, Lactobacillus acidophilus TYCA06, and Lactobacillus paracasei ET-66
    Jui-Fen Chen, Ko-Chiang Hsia, Yi-Wei Kuo, Shu-Hui Chen, Yen-Yu Huang, Ching-Min Li, Yu-Chieh Hsu, Shin-Yu Tsai, Hsieh-Hsun Ho
    Nutrients.2023; 16(1): 126.     CrossRef
  • In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling
    Mariano A. Molina, Karolina M. Andralojc, Martijn A. Huynen, William P. J. Leenders, Willem J. G. Melchers
    npj Biofilms and Microbiomes.2022;[Epub]     CrossRef
  • Self-Emulsified Nanoemulsion for Vaginal Administration: In Vitro Study of Effect on Lactobacillus acidophilus
    Prachi Jirwankar, Shruthi Gobbooru, Jun Shao
    Journal of Pharmaceutical Sciences.2020; 109(10): 3145.     CrossRef
  • Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on proliferation and apoptosis of gingival epithelial cells
    Jun-jun Zhao, Long Jiang, Ya-qin Zhu, Xi-ping Feng
    Advances in Medical Sciences.2019; 64(1): 54.     CrossRef
  • Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp.
    Monique S. do Carmo, Francisca M. F. Noronha, Mariana O. Arruda, Ênnio P. da Silva Costa, Maria R. Q. Bomfim, Andrea S. Monteiro, Thiago A. F. Ferro, Elizabeth S. Fernandes, Jorge A. Girón, Valério Monteiro-Neto
    Frontiers in Microbiology.2016;[Epub]     CrossRef
  • Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli
    Ammar Algburi, Anna Volski, Michael L. Chikindas
    Pathogens and Disease.2015;[Epub]     CrossRef
  • Computational methodology for predicting the landscape of the human–microbial interactome region level influence
    Edgar D. Coelho, André M. Santiago, Joel P. Arrais, José Luís Oliveira
    Journal of Bioinformatics and Computational Biology.2015; 13(05): 1550023.     CrossRef
  • Evaluation of antipathogenic activity and adherence properties of human Lactobacillus strains for vaginal formulations
    M.C. Verdenelli, M.M. Coman, C. Cecchini, S. Silvi, C. Orpianesi, A. Cresci
    Journal of Applied Microbiology.2014; 116(5): 1297.     CrossRef
  • Lactobacillus Decelerates Cervical Epithelial Cell Cycle Progression
    Katarina Vielfort, Linda Weyler, Niklas Söderholm, Mattias Engelbrecht, Sonja Löfmark, Helena Aro, Paul J. Galardy
    PLoS ONE.2013; 8(5): e63592.     CrossRef
  • 37,506 View
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Effects of Simple and Disposable Chicken Cages for Experimental Eimeria Infections
Jeongmi Yoo, Sung H. Kang, Jipseol Jeong, Woo H. Kim, Suk Kim, Hyun S. Lillehoj, Wongi Min
Korean J Parasitol 2011;49(3):299-302.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.299

During experimental Eimeria infections in chickens, facilities are often contaminated by fecal oocysts known to be highly resistant to both chemical and enzymatic treatments. Thus, studies using experimental Eimeria infections have been limited due to the difficulty of complete elimination of residual oocysts from both cages and facilities. To overcome this limitation, simple, inexpensive, and disposable cages were constructed from cardboard boxes and tested during experimental Eimeria maxima infections. The cages were used in animal rooms with only a 1.7% evidence of coccidia contamination between adjacent cages. No significant differences in fecal oocyst output and body weight gain were noted between animals housed in disposable cages and animals housed in wire control cages. This cage design is a useful means for preventing oocyst contamination during experimental conditions, suggesting that this disposable cage design could be used for other avian infectious disease studies.

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  • Different strategies for producing naturally soluble form of common cytokine receptor γ chain
    Jipseol Jeong, Woo H. Kim, Cherry P. Fernandez, Suk Kim, Yong-Hwan Kim, Hyung-Kwan Jang, Hyun S. Lillehoj, Hee-Jong Woo, Wongi Min
    Developmental & Comparative Immunology.2015; 48(1): 13.     CrossRef
  • Chicken IL-17F: Identification and comparative expression analysis in Eimeria-infected chickens
    Woo H. Kim, Jipseol Jeong, Ae R. Park, Dongjean Yim, Yong-Hwan Kim, Kwang D. Kim, Hong H. Chang, Hyun S. Lillehoj, Byung-Hyung Lee, Wongi Min
    Developmental & Comparative Immunology.2012; 38(3): 401.     CrossRef
  • Identification and Comparative Expression Analysis of Interleukin 2/15 Receptor β Chain in Chickens Infected with E. tenella
    Jipseol Jeong, Woo H. Kim, Jeongmi Yoo, Changhwan Lee, Suk Kim, Jae-Hyeon Cho, Hyung-Kwan Jang, Dong W. Kim, Hyun S. Lillehoj, Wongi Min, Ivan Cruz Moura
    PLoS ONE.2012; 7(5): e37704.     CrossRef
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Resistance to Toxoplasma gondii Infection in Mice Treated with Silk Protein by Enhanced Immune Responses
Joung-Ho Moon, Kyoung-Ho Pyo, Bong-Kwang Jung, Hyang Sook Chun, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2011;49(3):303-308.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.303

This study investigated whether elevated host immune capacity can inhibit T. gondii infection. For this purpose, we used silk protein extracted from Bombyx mori cocoons as a natural supplement to augment immune capacity. After silk protein administration to BALB/c mice for 6 weeks, ratios of T lymphocytes (CD4+ and CD8+ T-cells) and splenocyte proliferative capacities in response to Con A or T. gondii lysate antigen (TLA) were increased. Of various cytokines, which regulate immune systems, Th1 cytokines, such as IFN-γ, IL-2, and IL-12, were obviously increased in splenocyte primary cell cultures. Furthermore, the survival of T. gondii (RH strain)-infected mice increased from 2 days to 5 or more days. In a state of immunosuppression induced by methylprednisolone acetate, silk protein-administered mice were resistant to reduction in T-lymphocyte (CD4+ and CD8+ T-cells) numbers and the splenocyte proliferative capacity induced by Con A or TLA with a statistical significance. Taken together, our results suggest that silk protein augments immune capacity in mice and the increased cellular immunity by silk protein administration increases host protection against acute T. gondii infection.

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  • Oral Toxicity and Hypotensive Influence of Sericin-Derived Oligopeptides (SDOs) from Yellow Silk Cocoons of Bombyx mori in Rodent Studies
    Chainarong Tocharus, Virakboth Prum, Manote Sutheerawattananonda
    Foods.2024; 13(21): 3505.     CrossRef
  • Low dose administration of mature silkworm powder induces gastric mucosal defense factors in ethanol-induced gastric injury rat model
    Da-Young Lee, Moon-Young Song, Kyung-Sook Hong, Sun-Mi Yun, Young-Min Han, Eun-Hee Kim
    Food Science and Biotechnology.2023; 32(11): 1551.     CrossRef
  • Dietary administration of silk microparticles improves the epidermal and dermal regeneration after a skin wounding in gilthead seabream (Sparus aurata L.)
    Nora Albaladejo-Riad, Cristóbal Espinosa Ruiz, M. Ángeles Esteban
    Fish & Shellfish Immunology.2022; 124: 92.     CrossRef
  • The effect of silk peptide on immune system, A randomized, double-blind, placebo-controlled clinical trial
    Jin-Taek Hwang, Jung Min Cho, In Hye Jeong, Jeong-yong Lee, Ki-Chan Ha, Hyang-Im Baek, Hye Jeong Yang, Min Jung Kim, Jong Ho Lee
    Journal of Functional Foods.2019; 55: 275.     CrossRef
  • Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
    Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang
    Pharmaceutical Biology.2019; 57(1): 369.     CrossRef
  • Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells
    Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang
    Immune Network.2018;[Epub]     CrossRef
  • Biomaterials-Based Vaccination Strategies for the Induction of CD8+T Cell Responses
    Charles B. Chesson, Shaunte Ekpo-Otu, Janice J. Endsley, Jai S. Rudra
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    The Korean Journal of Parasitology.2013; 51(5): 589.     CrossRef
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Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea
Sung-Jong Hong, Chom-Kyu Chong, Kyutaeg Lee, Tong-Soo Kim, Yeon-Pyo Hong, Hye-Jin Ahn, Hak-Yong Kim, A-Ra Ko, Yong-Joo Kim, Ho-Woo Nam
Korean J Parasitol 2011;49(3):309-311.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.309

Seroepidemiological status of toxoplasmosis among the residents of Jeju island was surveyed and evaluated by ELISA with crude extract of Toxoplasma gondii. The sera of 2,348 residents (male 1,157 and female 1,191) were collected and checked for the IgG antibody titers, which showed 13.2% positive rate (309 sera). The positive rates were increasing gradually according to the age from 4.3% in teenage to 20.6% in seventies. The positive rates were significantly different between the sex by 16.2% for male and 10.2% for female (P<0.05). This positive rate of toxoplasmosis in Jeju island residents is regarded relatively higher than any other regions of Korea. And the high positive rate may be maintained continuously among Jeju island residents without any clear reasons until now but due to some parts peculiar socio-cultural tradition of Jeju island. Therefore, it is necessary to study further the epidemiology of toxoplasmosis of Jeju island.

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  • Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
    Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(1): 9.     CrossRef
  • Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
    Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2021; 59(3): 251.     CrossRef
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    Maria da Conceição Carvalho, Müller Ribeiro-Andrade, Renata Pimentel Bandeira de Melo, Dandara Matias Guedes, José Wilton Pinheiro Junior, Erika Fernanda Torres Samico Fernandes Cavalcanti, Fernando Jorge Rodrigues Magalhães, Rinaldo Aparecido Mota
    Revista Brasileira de Parasitologia Veterinária.2021;[Epub]     CrossRef
  • Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
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    The Korean Journal of Parasitology.2020; 58(3): 257.     CrossRef
  • Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
    Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
    The Korean Journal of Parasitology.2020; 58(5): 493.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
    Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(3): 247.     CrossRef
  • Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
    Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
    The Korean Journal of Parasitology.2017; 55(4): 385.     CrossRef

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  • Toxoplasma gondii Infection: Seroprevalence and Associated Risk Factors among Primary School Children in the Capital Area of the Republic of the Marshall Islands
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    Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
    The Korean Journal of Parasitology.2013; 51(5): 503.     CrossRef
  • Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
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    The Korean Journal of Parasitology.2012; 50(4): 287.     CrossRef
  • A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea
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    The Korean Journal of Parasitology.2012; 50(3): 191.     CrossRef
  • Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea
    Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
    The Korean Journal of Parasitology.2012; 50(3): 225.     CrossRef
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Mosquito Species Composition and Plasmodium vivax Infection Rates on Baengnyeong-do (Island), Republic of Korea
Desmond H. Foley, Terry A. Klein, In-Yong Lee, Myung-Soon Kim, Richard C. Wilkerson, Genelle Harrison, Leopoldo M. Rueda, Heung Chul Kim
Korean J Parasitol 2011;49(3):313-316.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.313

Vivax malaria is a significant military and civilian health threat in the north of the Republic of Korea (ROK). The island of Baengnyeong-do is the westernmost point of the ROK and is located close to the southwestern coast of the Democratic People's Republic of Korea (DPRK). Mosquitoes were collected using a black light trap on Baengnyeong-do, and Anopheles spp. were assayed by PCR, to identify the species, and screened for sporozoites of Plasmodium vivax. Of a subsample of 257 mosquitoes, Anopheles lesteri was the most frequently collected (49.8%), followed by Anopheles sinensis (22.6%), Anopheles pullus (18.7%), Anopheles kleini (7.8%), and Anopheles belenrae (1.2%). The overall sporozoite rate was 3.1%, with the highest rates observed in An. kleini (15.0%), An. sinensis (5.2%), and An. lesteri (1.6%). No sporozoite positive An. pullus or An. belenrae were observed. The results extend our knowledge of the distribution and potential role in malaria transmission of An. kleini, An. lesteri, and An. sinensis, for an area previously considered to be at a low risk for contracting vivax malaria.

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    Liwang Cui, Kami Kim
    Decoding Infection and Transmission.2024; 2: 100018.     CrossRef
  • Analysis of geometric morphometrics and molecular phylogeny for Anopheles species in the Republic of Korea
    Jiseung Jeon, Heung Chul Kim, Terry A. Klein, Kwang Shik Choi
    Scientific Reports.2023;[Epub]     CrossRef
  • Vector Competence and the Susceptibility of Anopheles pullus and Anopheles belenrae to Plasmodium vivax-Infected Blood From Thai Patients
    Ratawan Ubalee, Heung-Chul Kim, Siriporn Phasomkusolsil, Jaruwan Tawong, Ratree Takhampunya, Amnart Kayha, Suparat Chairuksa, Waranya Buadok, Vichit Phunkitchar, Betty K Poole-Smith, Silas A Davidson, Won-Ja Lee, Terry A Klein, Dina Fonseca
    Journal of Medical Entomology.2022; 59(3): 1047.     CrossRef
  • Species Diversity of Anopheles Mosquitoes and Plasmodium vivax Infection Rates, Gyeonggi Province, Republic of Korea During 2020
    So Youn Lee, Heung Chul Kim, Terry A Klein, Jihun Ryu, Min Hyeok Won, Jae Won Choi, Myung Soon Kim, Sung Tae Chong, Si Hyeock Lee, Young Ho Kim, Ju Hyeon Kim, Kwang Shik Choi, Nobuko Tuno
    Journal of Medical Entomology.2022; 59(5): 1778.     CrossRef
  • Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing
    Do Eun Lee, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Ju Hyeon Kim, Si Hyeock Lee
    Malaria Journal.2021;[Epub]     CrossRef
  • Monitoring Insecticide Resistance and Target Site Mutations of L1014 Kdr And G119 Ace Alleles in Five Mosquito Populations in Korea
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    The Korean Journal of Parasitology.2020; 58(5): 543.     CrossRef
  • Cost-Benefit Analysis of Malaria Chemoprophylaxis and Early Diagnosis for Korean Soldiers in Malaria Risk Regions
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    Journal of Korean Medical Science.2018;[Epub]     CrossRef
  • Comparison of Adult Mosquito Black-Light and Light-Emitting Diode Traps at Three Cowsheds Located in Malaria-Endemic Areas of the Republic of Korea
    Heung-Chul Kim, Myung-Soon Kim, Kwang-Shik Choi, Do-Un Hwang, Jaree L. Johnson, Terry A. Klein
    Journal of Medical Entomology.2017; 54(1): 221.     CrossRef
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    Desmond H. Foley, Terry A. Klein, Heung Chul Kim, Myung-Soon Kim, Richard C. Wilkerson, Cong Li, Genelle Harrison, Leopoldo M. Rueda
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  • Synchronous peaks in trap catches of malaria-infected mosquito species at Daeseongdong, a border village between North and South Korea
    Desmond H. Foley, Terry A. Klein, Heung Chul Kim, Myung-Soon Kim, Richard C. Wilkerson, Genelle Harrison, Leopoldo M. Rueda, Won-Ja Lee
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The Jeju Weasel, Mustela sibilica quelpartis, A New Definitive Host for Gnathostoma nipponicum Yamaguti, 1941
Ho-Choon Woo, Hong-Shik Oh, Shin-Hyeong Cho, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2011;49(3):317-321.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.317

Adult gnathostomes were discovered in the stomach of the Jeju weasel, Mustela sibilica quelpartis, road-killed in Jeju-do (Province). Their morphological characters were examined to identify the species. Total 50 gnathostome adults were collected from 6 out of 10 weasels examined. In infected weasels, 4-6 worms were grouped and embedded in each granulomatous gastric tumor, except 1 weasel. Male worms were 25.0×1.4 mm in average size, and had a tail with pedunculate papillae, a spicule, and minute tegumental spines. Females were 40.0×2.5 mm in average size, and had a tail without tegumental spines. Pointed and posteriorly curved hooklets were arranged in 8-10 rows on the head bulb. Tegumental spines were distributed from behind the head bulb to the middle portion of the body. The spines were different in size and shape by the distribution level of the body surface. Fertilized eggs were 65.5×38.9 ?m in average size, and had a mucoid plug at 1 pole. These gnathostomes from Jeju weasels were identified as Gnathostoma nipponicum Yamaguti, 1941. By the present study, it was confirmed for the first time that G. nipponicum is distributed in Jeju-do, the Republic of Korea, and the Jeju weasel, M. sibilica quelpartis, plays a crucial role for its definitive host.

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  • A case of vocal cord gnathostomiasis diagnosed with sectional morphologies in a histopathological specimen from a Chinese woman living in Korea
    Doo Sik Park, Eun Hyun Cho, Kyung Hoon Park, Soo Min Jo, Bumjung Park, Sun Huh
    Parasites, Hosts and Diseases.2023; 61(3): 298.     CrossRef
  • Mustela sibirica (Carnivora: Mustelidae)
    Chris J Law
    Mammalian Species.2018; 50(966): 109.     CrossRef
  • Gnathostoma spinigerum Infection in the Upper Lip of a Korean Woman: An Autochthonous Case in Korea
    Jae Hee Kim, Hyemi Lim, Young-Sang Hwang, Tae Yeon Kim, Eun Mee Han, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2013; 51(3): 343.     CrossRef
  • Discovery of LarvalGnathostoma nipponicumin Frogs and Snakes from Jeju-do (Province), Republic of Korea
    Ho-Choon Woo, Hong-Shik Oh, Shin-Hyeong Cho, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2011; 49(4): 445.     CrossRef
  • 11,425 View
  • 95 Download
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Enterobius vermicularis Eggs Discovered in Coprolites from a Medieval Korean Mummy
Dong Hoon Shin, Chang Seok Oh, Jong-Yil Chai, Hye-Jung Lee, Min Seo
Korean J Parasitol 2011;49(3):323-326.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.323

While the presence of pinworm eggs in archaeological samples has been reported by many researchers in the New World, those have been detected very scarcely in the Old World, especially in East Asian countries. In fact, many parasite species were recovered from the archeological remains in Korea, eggs of Enterobius vermicularis had not been found. Recently, a female mummy buried in the 17th century was discovered in the Joseon tomb from Dangjin-gun, Chungcheongnam-do, Korea. After rehydration process for 12 days, investigations were carried on the luminal surface of the colon. From them, 3 eggs of E. vermicularis were recovered. They were elliptical, transparent with a thin egg shell, 50.3±5.2 ?m (length) and 28.2±3.9 ?m (width) in size. This is the first discovery of E. vermicularis eggs in East Asia.

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    Scientific Reports.2024;[Epub]     CrossRef
  • Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
    Jong-Yil Chai, Min Seo, Dong Hoon Shin
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  • Reconstructing the history of helminth prevalence in the UK
    Hannah Ryan, Patrik G Flammer, Rebecca Nicholson, Louise Loe, Ben Reeves, Enid Allison, Christopher Guy, Inés Lopez Doriga, Tony Waldron, Don Walker, Claas Kirchhelle, Greger Larson, Adrian L Smith, Subash Babu
    PLOS Neglected Tropical Diseases.2022; 16(4): e0010312.     CrossRef
  • Intestinal helminths as a biomolecular complex in archaeological research
    Patrik G. Flammer, Adrian L. Smith
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  • Mummy studies and the bioarchaeology of care
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  • First description of Enterobius vermicularis egg in a coprolite dated from the pre-contact in Brazil
    Matheus Lino, Daniela Leles, Alfredo P. Peña, Marina C. Vinaud
    Journal of Archaeological Science: Reports.2018; 17: 1.     CrossRef
  • Discovery of Parasite Eggs in Archeological Residence during the 15th Century in Seoul, Korea
    Pyo Yeon Cho, Jung-Min Park, Myeong-Ki Hwang, Seo Hye Park, Yun-Kyu Park, Bo-Young Jeon, Tong-Soo Kim, Hyeong-Woo Lee
    The Korean Journal of Parasitology.2017; 55(3): 357.     CrossRef
  • A Case of Ectopic Paragonimiasis in a 17th Century Korean Mummy
    D. H. Shin, Y.-S. Kim, D. S. Yoo, M. J. Kim, C. S. Oh, J. H. Hong, E. Lee, J. Y. Chai, M. Seo
    Journal of Parasitology.2017; 103(4): 399.     CrossRef
  • Paleoparasitological study on the soil sediment samples from archaeological sites of ancient Silla Kingdom in Korean peninsula
    Myeung Ju Kim, Min Seo, Chang Seok Oh, Jong-Yil Chai, Jinju Lee, Gab-jin Kim, Won Young Ma, Soon Jo Choi, Karl Reinhard, Adauto Araujo, Dong Hoon Shin
    Quaternary International.2016; 405: 80.     CrossRef
  • Human Coprolites as a Source for Paleomicrobiology
    Sandra Appelt, Michel Drancourt, Matthieu Le Bailly, Michel Drancourt, Didier Raoult
    Microbiology Spectrum.2016;[Epub]     CrossRef
  • Joseon Funerary Texts Tested Using Ancient DNA Analysis of a Korean Mummy
    Chang Seok Oh, Bou‐Ja Koh, Dong Soo Yoo, Jun Bum Park, So Ri Min, Yi‐Suk Kim, Sang Sup Lee, Jianye Ge, Seung Bum Seo, Dong Hoon Shin
    The Anatomical Record.2015; 298(6): 1191.     CrossRef
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    You Soo Kim, Chang Seok Oh, Jong Ha Hong, Moon-Woo Seong, Dong Hoon Shin
    Korean Journal of Physical Anthropology.2015; 28(3): 137.     CrossRef
  • Paleoparasitological Studies on Mummies of the Joseon Dynasty, Korea
    Min Seo, Adauto Araujo, Karl Reinhard, Jong Yil Chai, Dong Hoon Shin
    The Korean Journal of Parasitology.2014; 52(3): 235.     CrossRef
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    Yi-Suk Kim, In Sun Lee, Go-Un Jung, Myeung Ju Kim, Chang Seok Oh, Dong Su Yoo, Won-Joon Lee, Eunju Lee, Soon Chul Cha, Dong Hoon Shin, Debbie Guatelli-Steinberg
    PLoS ONE.2014; 9(7): e99779.     CrossRef
  • The Changing Pattern of Parasitic Infection Among Korean Populations by Paleoparasitological Study of Joseon Dynasty Mummies
    Min Seo, Chang Seok Oh, Jong-Yil Chai, Mi Sook Jeong, Sung Woo Hong, Young-Min Seo, Dong Hoon Shin
    Journal of Parasitology.2014; 100(1): 147.     CrossRef
  • Collaboration of Archaeologists, Historians and Bioarchaeologists During Removal of Clothing from Korean Mummy of Joseon Dynasty
    Eun-Joo Lee, Chang Seok Oh, Se Gweon Yim, Jun Bum Park, Yi-Suk Kim, Myung Ho Shin, Soong Deok Lee, Dong Hoon Shin
    International Journal of Historical Archaeology.2013; 17(1): 94.     CrossRef
  • Food, parasites, and epidemiological transitions: A broad perspective
    K.J. Reinhard, L.F. Ferreira, F. Bouchet, L. Sianto, J.M.F. Dutra, A. Iniguez, D. Leles, M. Le Bailly, M. Fugassa, E. Pucu, A. Araújo
    International Journal of Paleopathology.2013; 3(3): 150.     CrossRef
  • Paleoparasitological Surveys for Detection of Helminth Eggs in Archaeological Sites of Jeolla-do and Jeju-do
    Myeong-Ju Kim, Dong Hoon Shin, Mi-Jin Song, Hye-Young Song, Min Seo
    The Korean Journal of Parasitology.2013; 51(4): 489.     CrossRef
  • 11,525 View
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Discovery of Taeniid Eggs from A 17th Century Tomb in Korea
Hye-Jung Lee, Dong-Hoon Shin, Min Seo
Korean J Parasitol 2011;49(3):327-329.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.327

Even though Taenia spp. eggs are occasionally discovered from archeological remains around the world, these eggs have never been discovered in ancient samples from Korea. When we attempted to re-examine the archeological samples maintained in our collection, the eggs of Taenia spp., 5 in total number, were recovered from a tomb of Gongju-si. The eggs had radially striated embryophore, and 37.5-40.0 ?m×37.5 ?m in size. This is the first report on taeniid eggs from ancient samples of Korea, and it is suggested that intensive examination of voluminous archeological samples should be needed for identification of Taenia spp.

Citations

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  • Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
    Jong-Yil Chai, Min Seo, Dong Hoon Shin
    Parasites, Hosts and Diseases.2023; 61(4): 345.     CrossRef
  • A comparison of ancient parasites as seen from archeological contexts and early medical texts in China
    Hui-Yuan Yeh, Xiaoya Zhan, Wuyun Qi
    International Journal of Paleopathology.2019; 25: 30.     CrossRef
  • Discovery of Parasite Eggs in Archeological Residence during the 15th Century in Seoul, Korea
    Pyo Yeon Cho, Jung-Min Park, Myeong-Ki Hwang, Seo Hye Park, Yun-Kyu Park, Bo-Young Jeon, Tong-Soo Kim, Hyeong-Woo Lee
    The Korean Journal of Parasitology.2017; 55(3): 357.     CrossRef
  • Paleoparasitological study on the soil sediment samples from archaeological sites of ancient Silla Kingdom in Korean peninsula
    Myeung Ju Kim, Min Seo, Chang Seok Oh, Jong-Yil Chai, Jinju Lee, Gab-jin Kim, Won Young Ma, Soon Jo Choi, Karl Reinhard, Adauto Araujo, Dong Hoon Shin
    Quaternary International.2016; 405: 80.     CrossRef
  • The Changing Pattern of Parasitic Infection Among Korean Populations by Paleoparasitological Study of Joseon Dynasty Mummies
    Min Seo, Chang Seok Oh, Jong-Yil Chai, Mi Sook Jeong, Sung Woo Hong, Young-Min Seo, Dong Hoon Shin
    Journal of Parasitology.2014; 100(1): 147.     CrossRef
  • Paleoparasitological Studies on Mummies of the Joseon Dynasty, Korea
    Min Seo, Adauto Araujo, Karl Reinhard, Jong Yil Chai, Dong Hoon Shin
    The Korean Journal of Parasitology.2014; 52(3): 235.     CrossRef
  • Paleoparasitological Surveys for Detection of Helminth Eggs in Archaeological Sites of Jeolla-do and Jeju-do
    Myeong-Ju Kim, Dong Hoon Shin, Mi-Jin Song, Hye-Young Song, Min Seo
    The Korean Journal of Parasitology.2013; 51(4): 489.     CrossRef
  • Food, parasites, and epidemiological transitions: A broad perspective
    K.J. Reinhard, L.F. Ferreira, F. Bouchet, L. Sianto, J.M.F. Dutra, A. Iniguez, D. Leles, M. Le Bailly, M. Fugassa, E. Pucu, A. Araújo
    International Journal of Paleopathology.2013; 3(3): 150.     CrossRef
  • 10,699 View
  • 88 Download
  • Crossref
Ticks Collected from Selected Mammalian Hosts Surveyed in the Republic of Korea During 2008-2009
Heung Chul Kim, Sang Hoon Han, Sung Tae Chong, Terry A. Klein, Chang-Yong Choi, Hyun-Young Nam, Hee-Young Chae, Hang Lee, Sungjin Ko, Jun-Gu Kang, Joon-Seok Chae
Korean J Parasitol 2011;49(3):331-335.
Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.331

A tick survey was conducted to determine the relative abundance and distribution of ticks associated with selected mammals in the Republic of Korea (ROK) during 2008-2009. A total of 918 ticks were collected from 76 mammals (6 families, 9 species) captured at 6 provinces and 3 Metropolitan Cities in ROK. Haemaphysalis longicornis (54.4%) was the most frequently collected tick, followed by Haemaphysalis flava (28.5%), Ixodes nipponensis (7.6%), Ixodes pomerantzevi (4.8%), Ixodes persulcatus (4.6%), and Haemaphysalis japonica (0.1%). Adults (57.0%) and nymphs (28.7%) of Ixodes and Haemaphysalis spp. were collected most frequently from medium or large mammals in this survey, while few larvae (14.3%) were collected. Hydropotes inermis was the most frequently captured mammal (52.6%), with a 16.4 tick index and 5 of 6 species of ticks collected during this survey. H. longicornis (69.7%) was the predominant tick collected from H. inermis, followed by H. flava (22.2%), I. persulcatus (6.1%), I. nipponensis (1.8%), and H. japonica (0.2%).

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