Parasites, Hosts and Diseases

Previous issues

Volume 42(4); December 2004

Original Articles

Developmental surface ultrastructure of Macroorchis spinulosus in albino rats: Sung-Jong Hong, Ho-Chun Woo, Oh Sil Kwon; Korean J Parasitol 2004;42(4):151-157.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.151

Abstract
PDF

Developmental surface ultrastructure of Macroorchis spinulosus was studied by scanning electron microscopy. One-day-old juvenile fluke was leaf-shaped and bent ventrally. Body surface was covered densely with peg-like spines and with cobblestone-like cytoplasmic processes. Ciliated sensory papillae were concentrated around oral sucker. Several unciliated sensory papillae occurred equidistantly on oral sucker and acetabulum. The ciliated papillae appeared in two longitudinal lines symmetric bilaterally on dorsal surface. On adult flukes, tegumantal spines became wider in middle of the body surface. The cytoplasmic processes differentiated into more fine velvety form. It is likely that the differentiated fine cytoplasmic processes are an increased absorptive surface to adult M. spinulosus. It is suggested that single pointed tegumental spines on anterior half of the body may be supportive for this fluke to migration.

Citations

Citations to this article as recorded by

Evaluation of the effect of larval metamorphosis of some digenetic parasites within the digestive gland tissues of the freshwater ampullariid snails, Lanistes carinatus (Olivier, 1804)
Mohammed E. El-Zeiny, Ola A. Abu Samak, Shereen A. Fahmy, Abdel Aziz A. Khidr
Parasitology International.2025; 108: 103078. CrossRef
Lethal effects of praziquantel and albendazole, on the cercariae of Echinochasmus sp. (Dietz, 1909) in-vitro
Mohammed E. El-Zeiny, Ola A. Abu Samak, Shereen A. Fahmy, Abdel Aziz A. Khidr
Journal of Parasitic Diseases.2024; 48(2): 320. CrossRef
Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences
Eun Jeong Won, Deok-Gyu Kim, Jaeeun Cho, Bong-Kwang Jung, Min-Jae Kim, Yong Woon Yun, Jong-Yil Chai, Dong Wook Ryang
The Korean Journal of Parasitology.2016; 54(1): 109. CrossRef
Surface ultrastructure of Plagiorchis muris growth and developmental stages in rats, the final host
Sung-Jong Hong
Parasitology Research.2009; 105(4): 1077. CrossRef
Surface ultrastructure of the plagiorchid trematode Glossidium pedatum Looss, 1899 from bagrid fish in Egypt
Mohammed Hasan Ibraheem
Acta Zoologica.2007; 88(2): 173. CrossRef
MORPHOLOGICAL OBSERVATIONS OF ECHINOCHASMUS JAPONICUS CERCARIAE AND THE IN VITRO MAINTENANCE OF ITS LIFE CYCLE FROM CERCARIAE TO ADULTS
Min-Ho Choi, Sae Hoon Kim, Jong-Hoon Chung, Hye-Jin Jang, Joon-Ho Eom, Byung-Suk Chung, Woon-Mok Sohn, Jong-Yil Chai, Sung-Tae Hong
Journal of Parasitology.2006; 92(2): 236. CrossRef

7,269 View
65 Download
Crossref

Codon usage and bias in mitochondrial genomes of parasitic platyhelminthes: Thanh Hoa Le, Donald Peter McManus, David Blair; Korean J Parasitol 2004;42(4):159-167.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.159

Abstract
PDF

Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood flukeSchistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.

Citations

Citations to this article as recorded by

Signatures of Endosymbiosis in Mitochondrial Genomes of Rhabdocoel Flatworms
M. Monnens, T. Artois, A. Briscoe, Y. L. Diez, K. P. P. Fraser, B. S. Leander, D. T. J. Littlewood, M. J. Santos, K. Smeets, N. W. L. Van Steenkiste, M. P. M. Vanhove
Molecular Ecology.2025;[Epub] CrossRef
Evolution of codon usage in Taenia saginata genomes and its impact on the host
Siddiq Ur Rahman, Hassan Ur Rehman, Inayat Ur Rahman, Muazzam Ali Khan, Fazli Rahim, Hamid Ali, Dekun Chen, Wentao Ma
Frontiers in Veterinary Science.2023;[Epub] CrossRef
Characterization of the complete mitochondrial genome of the fluke of turdus, Plagiorchis elegans, and phylogenetic implications
Rong Li, Hui-Mei Wang, Guo-Hua Liu, Ya Tu, Yuan-Ping Deng
Experimental Parasitology.2022; 242: 108387. CrossRef
Analysis of codon usage bias of lumpy skin disease virus causing livestock infection
Siddiq Ur Rahman, Hassan Ur Rehman, Inayat Ur Rahman, Abdur Rauf, Abdulrahman Alshammari, Metab Alharbi, Noor ul Haq, Hafiz Ansar Rasul Suleria, Sayed Haidar Abbas Raza
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Understanding the codon usage patterns of mitochondrial CO genes among Amphibians
Parvin A. Barbhuiya, Arif Uddin, Supriyo Chakraborty
Gene.2021; 777: 145462. CrossRef
Characterization of the complete mitochondrial genome of Diplostomum baeri
Toby Landeryou, Stephen M. Kett, Anne Ropiquet, Dirk Wildeboer, Scott P. Lawton
Parasitology International.2020; 79: 102166. CrossRef
Probing recalcitrant problems in polyclad evolution and systematics with novel mitochondrial genome resources
Nathan J. Kenny, Carolina Noreña, Cristina Damborenea, Cristina Grande
Genomics.2019; 111(3): 343. CrossRef
The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes
Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Takeshi Agatsuma, David Blair
PeerJ.2019; 7: e7031. CrossRef
Compositional Analysis of Flatworm Genomes Shows Strong Codon Usage Biases Across All Classes
Guillermo Lamolle, Santiago Fontenla, Gastón Rijo, Jose F. Tort, Pablo Smircich
Frontiers in Genetics.2019;[Epub] CrossRef
Compositional bias coupled with selection and mutation pressure drives codon usage in Brassica campestris genes
Prosenjit Paul, Arup Kumar Malakar, Supriyo Chakraborty
Food Science and Biotechnology.2018; 27(3): 725. CrossRef
Phenotypic plasticity of six unusual cercariae in nassariid gastropods and their relationships to the Acanthocolpidae and Brachycladiidae (Digenea)
Leonie J. Barnett, Terrence L. Miller
Parasitology International.2018; 67(2): 225. CrossRef
Characterization of the complete mitochondrial genomes from Polycladida (Platyhelminthes) using next-generation sequencing
M. Teresa Aguado, Cristina Grande, Michael Gerth, Christoph Bleidorn, Carolina Noreña
Gene.2016; 575(2): 199. CrossRef
The complete mitochondrial genome of the tapeworm Cladotaenia vulturi (Cestoda: Paruterinidae): gene arrangement and phylogenetic relationships with other cestodes
Aijiang Guo
Parasites & Vectors.2016;[Epub] CrossRef
A complete mitochondrial genome from Echinochasmus japonicus supports the elevation of Echinochasminae Odhner, 1910 to family rank (Trematoda: Platyhelminthes)
Thanh Hoa Le, Nga Thi Bich Nguyen, Khue Thi Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David Blair
Infection, Genetics and Evolution.2016; 45: 369. CrossRef
Evolutionary Analysis of Mitogenomes from Parasitic and Free-Living Flatworms
Eduard Solà, Marta Álvarez-Presas, Cristina Frías-López, D. Timothy J. Littlewood, Julio Rozas, Marta Riutort, Hector Escriva
PLOS ONE.2015; 10(3): e0120081. CrossRef
An initial molecular signature of Indian isolates of Toxocara
K JEEVA, N CHATTERJEE, A K BERA, S MAITI, HIRA RAM, P S BANERJEE, T K GOSWAMI, P J DAS, J CHAMUA, A A P MILTON, M ARAVIND, G BHUVANAPRIYA, K THILAGAVATHI, S K GHOSH, S M DEB, D BHATTACHARYA
The Indian Journal of Animal Sciences.2015;[Epub] CrossRef
Genetic diversity of the Chinese liver fluke Clonorchis sinensis from Russia and Vietnam
Galina N. Chelomina, Yulia V. Tatonova, Nguyen Manh Hung, Ha Duy Ngo
International Journal for Parasitology.2014; 44(11): 795. CrossRef
Mutation and Selection Cause Codon Usage and Bias in Mitochondrial Genomes of Ribbon Worms (Nemertea)
Haixia Chen, Shichun Sun, Jon L. Norenburg, Per Sundberg, Tamir Tuller
PLoS ONE.2014; 9(1): e85631. CrossRef
Platyzoan mitochondrial genomes
Alexandra R. Wey-Fabrizius, Lars Podsiadlowski, Holger Herlyn, Thomas Hankeln
Molecular Phylogenetics and Evolution.2013; 69(2): 365. CrossRef
Analysis of synonymous codon usage patterns in different plant mitochondrial genomes
Meng Zhou, Xia Li
Molecular Biology Reports.2009; 36(8): 2039. CrossRef
DNA Asymmetric Strand Bias Affects the Amino Acid Composition of Mitochondrial Proteins
Xiang Jia Min, Donal A. Hickey
DNA Research.2007; 14(5): 201. CrossRef
Characterization of the Complete Mitochondrial Genome of Diphyllobothrium nihonkaiense (Diphyllobothriidae: Cestoda), and Development of Molecular Markers for Differentiating Fish Tapeworms
Kyu-Heon Kim, Hyeong-Kyu Jeon, Seokha Kang, Tahera Sultana, Gil Jung Kim, Keeseon S. Eom, Joong-Ki Park
Molecules and Cells.2007; 23(3): 379. CrossRef
Comparison of the phylogenetic performance of neodermatan mitochondrial protein‐coding genes
Michael Hardman, Lotta M. Hardman
Zoologica Scripta.2006; 35(6): 655. CrossRef
Complete mitochondrial genome sequences for Crown-of-thorns starfish Acanthaster planci and Acanthaster brevispinus
Nina Yasuda, Masami Hamaguchi, Miho Sasaki, Satoshi Nagai, Masaki Saba, Kazuo Nadaoka
BMC Genomics.2006;[Epub] CrossRef

10,144 View
89 Download
Crossref

Organ-specific antigens of Clonorchis sinensis: Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2004;42(4):169-174.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.169

Abstract
PDF

This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

Citations

Citations to this article as recorded by

Identification of Myoferlin, a Potential Serodiagnostic Antigen of Clonorchiasis, via Immunoproteomic Analysis of Sera From Different Infection Periods and Excretory-Secretory Products of Clonorchis sinensis
Xiao-Xiao Ma, Yang-Yuan Qiu, Zhi-Guang Chang, Jun-Feng Gao, Rui-Ruo Jiang, Chun-Lin Li, Chun-Ren Wang, Qiao-Cheng Chang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis
Soon Bin Kwon, Paul Kim, Hae Sun Woo, Tae Yun Kim, Ju Yeong Kim, Hye Min Lee, Yun Soo Jang, Eun-Min Kim, Tai-Soon Yong, Baik Lin Seong
Parasitology.2018; 145(12): 1531. CrossRef
Characterization of immunogenic Clonorchis sinensis protein fractions by gel filtration chromatography
Duan Pham Ngoc, Yuji Arimatsu, Sasithorn Kaewkes, Banchob Sripa
Asian Pacific Journal of Tropical Disease.2015; 5(4): 284. CrossRef
Free radicals enzymatically triggered by Clonorchis sinensis excretory–secretory products cause NF-κB-mediated inflammation in human cholangiocarcinoma cells
Joo-Hyun Nam, Ju Hyun Moon, In Ki Kim, Myoung-Ro Lee, Sung-Jong Hong, Joong Ho Ahn, Jong Woo Chung, Jhang Ho Pak
International Journal for Parasitology.2012; 42(1): 103. CrossRef
In Vitro Maintenance of Clonorchis sinensis Adult Worms
Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2012; 50(4): 309. CrossRef
Production and Deformation of Clonorchis sinensis Eggs during In Vitro Maintenance
Md. Hafiz Uddin, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong, Jason Mulvenna
PLoS ONE.2012; 7(12): e52676. CrossRef
KOMP'YuTERNYY I EKSPERIMENTAL'NYY ANALIZ ANTIGENNYKh SVOYSTV VITELLINA I KATEPSINA F OPISTHORCHIS FELINEUS
A A Nosova, P A Belavin, D A Afonnikov
Medical academic journal.2012; 12(4): 33. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Scientific Opinion on risk assessment of parasites in fishery products

EFSA Journal.2010;[Epub] CrossRef
Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products
Jhang Ho Pak, Dong-Wook Kim, Ju Hyun Moon, Joo-Hyun Nam, Jong-Hyeok Kim, Jung Won Ju, Tong-Soo Kim, Sang-Beom Seo
Parasitology Research.2009; 104(5): 1035. CrossRef
Proteomic analysis of differentially expressed proteins in human cholangiocarcinoma cells treated withClonorchis sinensisexcretory–secretory products
Jhang Ho Pak, Ju Hyun Moon, Seung‐Jun Hwang, Shin‐Hyeong Cho, Sang‐Beom Seo, Tong‐Soo Kim
Journal of Cellular Biochemistry.2009; 108(6): 1376. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media
Shunyu Li, Tae Im Kim, Won Gi Yoo, Pyo Yun Cho, Tong-Soo Kim, Sung-Jong Hong
Parasitology Research.2008; 103(5): 1019. CrossRef
Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis
Changling Ma, Xuchu Hu, Fengyu Hu, Yanwen Li, Xiaoxiang Chen, Zhenwen Zhou, Fangli Lu, Jin Xu, Zhongdao Wu, Xinbing Yu
Parasitology Research.2007; 101(2): 419. CrossRef
Clonorchis sinensis: Expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue
Fengyu Hu, Xinbing Yu, Changling Ma, Hongjuan Zhou, Zhenwen Zhou, Yanwen Li, Fangli Lu, Jin Xu, Zhongdao Wu, Xuchu Hu
Experimental Parasitology.2007; 117(2): 157. CrossRef
Long-term storage of Clonorchis sinensis metacercariae in vitro
Shunyu Li, Hyoun Woo Kang, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2006; 100(1): 25. CrossRef
Fish-borne parasitic zoonoses: Status and issues
Jong-Yil Chai, K. Darwin Murrell, Alan J. Lymbery
International Journal for Parasitology.2005; 35(11-12): 1233. CrossRef

8,921 View
59 Download
Crossref

Effects of iNOS inhibitor on IFN-γ production and apoptosis of splenocytes in genetically different strains of mice infected with Toxoplasma gondii: Ki-Man Kang, Gye-Sung Lee, Jae-Ho Lee, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2004;42(4):175-183.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.175

Abstract
PDF

To evaluate the role of nitric oxide (NO) in IFN-γ production and apoptosis of splenocytes in genetically different strains of mice with toxoplasmosis, BALB/c (a toxoplasmosis resistant strain) and C57BL/6 (a toxoplasmosis susceptible strain) mice were infected with Toxoplasma gondii cysts orally and subsequently injected intraperitoneally with aminoguanidine, an iNOS inhibitor (AG; 35 mg/kg per mouse daily for 14 days). When BALB/c or C57BL/6 mice were infected with T. gondii without AG treatment, number of brain cysts, NO and IFN-γ production by splenocytes, and percentages of apoptotic splenocytes were increased compared to uninfected control mice without AG treatment. AG treatment increased the number of brain cysts, and reduced NO and IFN-γ production in T. gondii-infected C57BL/6 mice. In contrast, in T. gondii-infected BABL/c mice, the number of brain cysts, and NO and IFN-γ production of splenocytes was not altered by treatment with AG. However, the percentages of apoptotic splenocytes in T. gondii-infected BALB/c or C57BL/6 mice were not affected by AG treatment. These results suggest that NO modulates IFN-γ production in T. gondii-infected C57BL/6 mice, and that NO is involved in mediating a protective response in toxoplasmosis susceptible, but not resistant, mice strain during acute infection.

Citations

Citations to this article as recorded by

Effect of spiramycin versus aminoguanidine and their combined use in experimental toxoplasmosis
Marwa Omar, Beessa E. Abaza, Esraa Mousa, Shereen M. Ibrahim, Hayam E. Rashed, Tahani I. Farag
Journal of Parasitic Diseases.2021; 45(4): 1014. CrossRef
Recombinant TgHSP70 Immunization Protects against Toxoplasma gondii Brain Cyst Formation by Enhancing Inducible Nitric Oxide Expression
Paulo Czarnewski, Ester C. B. Araújo, Mário C. Oliveira, Tiago W. P. Mineo, Neide M. Silva
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
Pathophysiological mechanisms of diarrhea caused by theVibrio choleraeO1 El Tor variant: anin vivostudy in mice
Saravut Satitsri, Pawin Pongkorpsakol, Potjanee Srimanote, Varanuj Chatsudthipong, Chatchai Muanprasat
Virulence.2016; 7(7): 789. CrossRef
Oxidative stress and tryptophan degradation pattern of acute Toxoplasma gondii infection in mice
Ayse Basak Engin, Funda Dogruman-Al, Ugur Ercin, Bekir Celebi, Cahit Babur, Neslihan Bukan
Parasitology Research.2012; 111(4): 1725. CrossRef
Distinct roles for nitric oxide in resistant C57BL/6 and susceptible BALB/c mice to control Burkholderia pseudomallei infection
Katrin Breitbach, Patimaporn Wongprompitak, Ivo Steinmetz
BMC Immunology.2011;[Epub] CrossRef
MMHD [(S,E)-2-Methyl-1-(2-methylthiazol-4-yl) hexa-1,5-dien-ol], a Novel Synthetic Compound Derived From Epothilone, Suppresses Nuclear Factor-κB–Mediated Cytokine Expression in Lipopolysaccharide-Stimulated BV-2 Microglia
Na-Ra Jeon, Sushruta Koppula, Byung-Wook Kim, Su-Ho Park, Hyo-Won Lee, Dong-Kug Choi
Journal of Pharmacological Sciences.2010; 112(2): 158. CrossRef
The immunobiology of the innate response to Toxoplasma gondii
Catherine M. Miller, Nicola R. Boulter, Rowan J. Ikin, Nicholas C. Smith
International Journal for Parasitology.2009; 39(1): 23. CrossRef
Malondialdehyde, Glutathione, and Nitric Oxide Levels in Toxoplasma gondii Seropositive Patients
Ulku Karaman, Tuncay Celik, Tugba Raika Kiran, Cemil Colak, Nilgun Ulfet Daldal
The Korean Journal of Parasitology.2008; 46(4): 293. CrossRef
Toxoplasma gondii infection induces apoptosis in noninfected macrophages: role of nitric oxide and other soluble factors
Y. NISHIKAWA, O. KAWASE, O. VIELEMEYER, H. SUZUKI, K. A. JOINER, X. XUAN, H. NAGASAWA
Parasite Immunology.2007; 29(7): 375. CrossRef

8,891 View
114 Download
Crossref

Influence of calcium ion on host cell invasion and intracellular replication by Toxoplasma gondii: Hyun-Ouk Song, Myoung-Hee Ahn, Jae-Sook Ryu, Duk-Young Min, Kyoung-Hwan Joo, Young-Ha Lee; Korean J Parasitol 2004;42(4):185-193.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.185

Abstract
PDF

Toxoplasma gondii is an obligate intracellular protozoan parasite, which invades a wide range of hosts including humans. The exact mechanisms involved in its invasion are not fully understood. This study focused on the roles of Ca²⁺ in host cell invasion and in T. gondii replication. We examined the invasion and replication of T. gondii pretreated with several calcium modulators, the conoid extrusion of tachyzoites. Calmodulin localization in T. gondii were observed using the immunogold method, and Ca²⁺ levels in tachyzoites by confocal microscopy. In light microscopic observation, tachyzoites co-treated with A23187 and EGTA showed that host cell invasion and intracellular replication were decreased. The invasion of tachyzoites was slightly inhibited by the Ca²⁺ channel blockers, bepridiland verapamil, and by the calmodulin antagonist, calmidazolium. We observed that calcium saline containing A23187 induced the extrusion of tachyzoite conoid. By immunoelectron microscopy, gold particles bound to anti-calmodulin or anti-actin mAb, were found to be localized on the anterior portion of tachyzoites. Remarkably reduced intracellular Ca²⁺ was observed in tachyzoites treated with BAPTA/AM by confocal microscopy. These results suggest that host cell invasion and the intracellular replication of T. gondii tachyzoites are inhibited by the calcium ionophore, A23187, and by the extracellular calcium chelator, EGTA.

Citations

Citations to this article as recorded by

Unveiling the impact of cGMP-dependent protein kinase of Neospora caninum on calcium fluxes and egress functions through quantitative phosphoproteome analysis
Xianmei Wang, Kun Guo, Zhili Shan, Zhu Ying, Zifu Zhu, Shiman Yang, Na Yang, Qun Liu, Lifang Wang, Jing Liu
Communications Biology.2025;[Epub] CrossRef
Calreticulin (CALR) promotes ionophore-induced microneme secretion in Toxoplasma gondii
Zhili Shan, Xingju Song, Xu Yang, Yangfei Xue, Yayun Wu, Xianmei Wang, Jing Liu, Qun Liu
Parasitology Research.2024;[Epub] CrossRef
Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
Hyunho Kim, Sung-Hee Hong, Hyo Eun Jeong, Sewoon Han, Jinchul Ahn, Jin-A. Kim, Ji-Hun Yang, Hyun Jeong Oh, Seok Chung, Sang-Eun Lee
Scientific Reports.2022;[Epub] CrossRef
Toxoplasma gondiiinfection and risk of attention-deficit hyperactivity disorder: a systematic review and meta-analysis
Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Zahra Hosseininejad, Afsaneh Amouei, Ahmad Daryani
Pathogens and Global Health.2020; 114(3): 126. CrossRef
Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of verapamil treatment in vitro
M.S.R. Harun, Victoria Marsh, Nashwa A. Elsaied, Kevin F. Webb, Hany M. Elsheikha
Brain Research.2020; 1746: 147002. CrossRef
Resistance towards monensin is proposed to be acquired in aToxoplasma gondiimodel by reduced invasion and egress activities, in addition to increased intracellular replication
AHMED THABET, JOHANNES SCHMIDT, SVEN BAUMANN, WALTHER HONSCHA, MARTIN VON BERGEN, ARWID DAUGSCHIES, BERIT BANGOURA
Parasitology.2018; 145(3): 313. CrossRef
Anti-Toxoplasmosis Effect of the Herbal Extracts Plantago asiatica L.
Sunhwa Hong, Okjin Kim
Korean Journal of Plant Resources.2016; 29(1): 26. CrossRef
Anti-Toxoplasmosis effects of herbal extracts against Toxoplasma Gondii
Sunhwa Hong, Hyun-A Lee, Yung-Ho Chung, Tae-Wan Kim, Okjin Kim
Korean Journal of Veterinary Service.2014; 37(2): 101. CrossRef
Anti-Toxoplasmosis Effect of Meliae fructus Ethanol Extract
Hak-Yong Lee, Okjin Kim
Journal of Biomedical Research.2014; 15(4): 189. CrossRef
Anti-Toxoplasmosis Effect of the Halophyte Suaeda maritime
Sunhwa Hong, Hyun-A Lee, Yun-Seong Lee, Dong-Woo Kim, Jae-Hyeok Jeong, Tae-Wan Kim, Okjin Kim
Korean Journal of Plant Resources.2014; 27(5): 415. CrossRef
Anti-toxoplasmosis effect of Dictamnus dasycarpus extract against Toxoplasma Gondii
Sunhwa Hong, Hyun-A Lee, Yun-seong Lee, Yung-Ho Chung, Okjin Kim
Journal of Biomedical Research.2014; 15(1): 7. CrossRef
Interplay of host–pathogen microvesicles and their role in infectious disease
Jameel M. Inal, Ephraim A. Ansa-Addo, Sigrun Lange
Biochemical Society Transactions.2013; 41(1): 258. CrossRef
The effect of kinase, actin, myosin and dynamin inhibitors on host cell egress by Toxoplasma gondii
Lucio Ayres Caldas, Sergio Henrique Seabra, Márcia Attias, Wanderley de Souza
Parasitology International.2013; 62(5): 475. CrossRef
Toxoplasmosis and Polygenic Disease Susceptibility Genes: ExtensiveToxoplasma gondiiHost/Pathogen Interactome Enrichment in Nine Psychiatric or Neurological Disorders
C. J. Carter
Journal of Pathogens.2013; 2013: 1. CrossRef
In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii
Xiaoyan Yang, Bo Huang, Jianping Chen, Shiguang Huang, Huanqin Zheng, Zhao-Rong Lun, Jilong Shen, Yong Wang, Fangli Lu
Parasitology Research.2012; 110(6): 2221. CrossRef
Simple and efficient model systems of screening anti-Toxoplasmadrugsin vitro
Chunmei Jin, Suk-Yul Jung, Sung Yeon Kim, Hyun-Ok Song, Hyun Park
Expert Opinion on Drug Discovery.2012; 7(3): 195. CrossRef
Identification of Putative Potassium Channel Homologues in Pathogenic Protozoa
David L. Prole, Neil V. Marrion, Gordon Langsley
PLoS ONE.2012; 7(2): e32264. CrossRef
In vitro and experimental therapeutic studies of the calcium channel blocker bepridil: Detection of viable Leishmania (L.) chagasi by real-time PCR
Juliana Q. Reimão, Fábio A. Colombo, Vera L. Pereira-Chioccola, André G. Tempone
Experimental Parasitology.2011; 128(2): 111. CrossRef
Investigation into in vitro anti-leishmanial combinations of calcium channel blockers and current anti-leishmanial drugs
Juliana Quero Reimão, André Gustavo Tempone
Memórias do Instituto Oswaldo Cruz.2011; 106(8): 1032. CrossRef
Microscopic analysis of calcium ionophore activated egress of Toxoplasma gondii from the host cell
Lucio Ayres Caldas, Wanderley de Souza, Márcia Attias
Veterinary Parasitology.2010; 167(1): 8. CrossRef
Induction and regulation of conoid extrusion inToxoplasma gondii
M. González Del Carmen, M. Mondragón, S. González, R. Mondragón
Cellular Microbiology.2009; 11(6): 967. CrossRef
THE IMPORTANCE OF TOXOPLASMA GONDII INFECTION IN DISEASES PRESENTING WITH HEADACHES. HEADACHES AND ASEPTIC MENINGITIS MAY BE MANIFESTATIONS OF THE JARISCH-HERXHEIMER REACTION
JOSEPH PRANDOTA
International Journal of Neuroscience.2009; 119(12): 2144. CrossRef
Idiopatyczne nadciśnienie śródczaszkowe, guz rzekomy czaszki oraz aseptyczne zapalenie opon mogą być spowodowane reaktywacją latentnej toksoplazmozy mózgowej
Józef Prandota
Pediatria Polska.2009; 84(5): 453. CrossRef
Characterization of a New Gene <italic>wx2</italic> in <italic>Toxoplasma gondii</italic>
Xiang Wu, Qiong Zhang, Kui Tan, Ronghua Xie, Jiubo Fan, Hengping Shu, Shiping Wang
Acta Biochimica et Biophysica Sinica.2007; 39(7): 475. CrossRef
Laser scanning cytometer‐based assays for measuring host cell attachment and invasion by the human pathogen Toxoplasma gondii
Jeffrey Mital, Janet Schwarz, Douglas J. Taatjes, Gary E. Ward
Cytometry Part A.2006; 69A(1): 13. CrossRef

10,159 View
96 Download
Crossref

Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits: Myung-Jo You; Korean J Parasitol 2004;42(4):195-200.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.195

Abstract
PDF

We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

Citations

Citations to this article as recorded by

Comprehensive antigen identification and comparative analysis: significant approaches for controlling Haemaphysalis longicornis ticks
Md. Samiul Haque, Bumseok Kim, Myung-Jo You
Journal of Veterinary Science.2025;[Epub] CrossRef
De Novo Transcriptome Sequencing and Profiling of Ovarian Development of Argas persicus Along the Trophogonic Cycle
Fen Yan, Deyong Duan, Jinzhu Meng, Tianyin Cheng
Genes.2025; 16(9): 1107. CrossRef
Hemalin vaccination modulates the host immune response and reproductive cycle of Haemaphysalis longicornis
Hejia Ma, Jingkai Ai, Yansha La, Xinyuan Zhao, Ankang Zeng, Qi Qin, Shangjiali Feng, Ming Kang, Yali Sun, Jixu Li
Veterinary Parasitology.2023; 323: 110051. CrossRef
Molecular cloning, expression and impact of ribosomal protein S-27 silencing in Haemaphysalis longicornis (Acari: Ixodidae)
Md Khalesur Rahman, Bumseok Kim, Myungjo You
Experimental Parasitology.2020; 209: 107829. CrossRef
Designing and Modeling of Complex DNA Vaccine Based on Tropomyosin Protein of Boophilus Genus Tick
Mohamamd Mahdi Ranjbar, Shishir K. Gupta, Khodayar Ghorban, Sedigheh Nabian, Alireza Sazmand, Mohammad Taheri, Sahar Esfandyari, Maryam Taheri
Applied Biochemistry and Biotechnology.2015; 175(1): 323. CrossRef
Identification of the tropomyosin (HL-Tm) in Haemaphysalis longicornis
Meiyuan Tian, Zhancheng Tian, Jin Luo, Junren Xie, Hong Yin, Qiaoying Zeng, Hui Shen, Huiping Chai, Xiaosong Yuan, Fangfang Wang, Guangyuan Liu
Veterinary Parasitology.2015; 207(3-4): 318. CrossRef
Identification of salivary antigenic markers discriminating host exposition between two European ticks: Rhipicephalus sanguineus and Dermacentor reticulatus
Vinh Vu Hai, Lionel Almeras, Stephane Audebert, Matthieu Pophillat, Nathalie Boulanger, Philippe Parola, Didier Raoult, Frederic Pages
Comparative Immunology, Microbiology and Infectious Diseases.2013; 36(1): 39. CrossRef
Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine
Runhui Zhang, Quwu Jise, Wanpeng Zheng, Yongjun Ren, Xiang Nong, Xuhang Wu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Songjia Lai, Guangyou Yang
Parasites & Vectors.2012;[Epub] CrossRef
Cloning, expression and evaluation of the efficacy of a recombinant Haemaphysalis concinna Hc-23 antigen in rabbits
Yao Bian, Weizhong Chen, Guangyou Yang, Xiaobin Gu, Shuxian Wang, Jiagang Sun, Kaijun Li, Hong Wei
Vaccine.2011; 29(5): 1041. CrossRef
Cloning and characterization of a cDNA clone encoding troponin T from tick Haemaphysalis qinghaiensis (Acari: Ixodidae)
Jinliang Gao, Jianxun Luo, Ruiquan Fan, Guiquan Guan, Volker Fingerle, Chihiro Sugimoto, Noboru Inoue, Hong Yin
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology.2008; 151(3): 323. CrossRef
Molecular characterization of a myosin alkali light chain-like protein, a “concealed” antigen from the hard tick Haemaphysalis qinghaiensis
Jinliang Gao, Jianxun Luo, Ruiquan Fan, Guiquan Guan, Qiaoyun Ren, Miling Ma, Chihiro Sugimoto, Qi Bai, Hong Yin
Veterinary Parasitology.2007; 147(1-2): 140. CrossRef
A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer)*
A. J. NISBET, J. F. HUNTLEY, A. MacKELLAR, N. SPARKS, R. MCDEVITT
Parasite Immunology.2006; 28(8): 401. CrossRef

8,825 View
85 Download
Crossref

Brief Communications

Status of intestinal parasite infections among children in Bat Dambang, Cambodia: Seung Kyu Park, Dong-Heui Kim, Young-Kun Deung, Hun-Joo Kim, Eun-Ju Yang, Soo-Jung Lim, Yong-Suk Ryang, Dan Jin, Kyu-Jae Lee; Korean J Parasitol 2004;42(4):201-203.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.201

Abstract
PDF

A survey was conducted to determine the extent of intestinal parasite infection in Bat Dambang, Cambodia in March 2004. A total of 623 fecal specimens was collected from kindergarten and schoolchildren and examined using the formalin-ether sedimentation technique. The overall infection rate of intestinal parasites was 25.7% (boys, 26.2%; girls, 25.1%), and the infection rates of intestinal helminthes by species were as follows: Echinostoma sp. 4.8%, hookworm 3.4%, Hymenolepis nana 1.3%, and Rhabditis sp. 1.3%. The infection rates of intestinal protozoa were; Entamoeba coli 4.8%, Giardia lamblia 2.9%, Iodamoeba butschlii 1.4%, Entamoeba polecki 1.1%, and Entamoeba histolytica 0.8%. There were no egg positive cases of Ascaris lumbricoides or Trichuris trichiura. All children infected were treated with albendazole, praziquantel, or metronidazole according to parasite species. The results showed that intestinal parasites are highly endemic in Bat Dambang, Cambodia.

Citations

Citations to this article as recorded by

Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single‐Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as ‘Non‐Pathogenic’
Christen Rune Stensvold
APMIS.2025;[Epub] CrossRef
Retrospective and Histopathological Studies of Entamoeba spp. and Other Pathogens Associated with Diarrhea and Wasting in Pigs in Aichi Prefecture, Japan
Tetsuya KOMATSU, Makoto MATSUBAYASHI, Naoko MURAKOSHI, Kazumi SASAI, Tomoyuki SHIBAHARA
Japan Agricultural Research Quarterly: JARQ.2019; 53(1): 59. CrossRef
Prevalence of Hookworm infection and Strongyloidiasis in Cats and Potential Risk Factor of Human Diseases
Blego Sedionoto, Witthaya Anamnart, Hadiyanto, Sudarno, Maryono
E3S Web of Conferences.2018; 31: 06002. CrossRef
Intestinal parasitic infections: Current prevalence and risk factors among schoolchildren in capital area of the Republic of Marshall Islands
Chien-Wei Liao, Ting-Wu Chuang, Ying-Chieh Huang, Chia-Mei Chou, Chia-Lien Chiang, Fei-Peng Lee, Yun-Ting Hsu, Jia-Wei Lin, Kennar Briand, Chia-Ying Tu, Chia-Kwung Fan
Acta Tropica.2017; 176: 242. CrossRef
Prevalence of Intestinal Protozoans among Schoolchildren in Suburban Areas near Yangon, Myanmar
Min-Jae Kim, Bong-Kwang Jung, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Keon-Hoon Lee, Seon Cho, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai
The Korean Journal of Parasitology.2016; 54(3): 345. CrossRef
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children
Catrin E. Moore, Kristin Elwin, Nget Phot, Chanthou Seng, Saroeun Mao, Kuong Suy, Varun Kumar, Johanna Nader, Rachel Bousfield, Sanuki Perera, J. Wendi Bailey, Nicholas J. Beeching, Nicholas P. J. Day, Christopher M. Parry, Rachel M. Chalmers, James S McC
PLOS Neglected Tropical Diseases.2016; 10(7): e0004822. CrossRef
High prevalence of large trematode eggs in schoolchildren in Cambodia
Philipp J. Bless, Fabian Schär, Virak Khieu, Stefanie Kramme, Sinuon Muth, Hanspeter Marti, Peter Odermatt
Acta Tropica.2015; 141: 295. CrossRef
Potentially Zoonotic Helminthiases of Murid Rodents from the Indo-Chinese Peninsula: Impact of Habitat and the Risk of Human Infection
Kittipong Chaisiri, Praphaiphat Siribat, Alexis Ribas, Serge Morand
Vector-Borne and Zoonotic Diseases.2015; 15(1): 73. CrossRef
A survey for potentially zoonotic gastrointestinal parasites of dogs and pigs in Cambodia
Tawin Inpankaew, K. Darwin Murrell, Nongnuch Pinyopanuwat, Chamnan Chhoun, Kuong Khov, Tharin Sem, San Sorn, Sinuon Muth, Anders Dalsgaard
Acta Parasitologica.2015;[Epub] CrossRef
Intestinal Parasite Infections in Symptomatic Children Attending Hospital in Siem Reap, Cambodia
Catrin E. Moore, Phot Nget, Mao Saroeun, Suy Kuong, Seng Chanthou, Varun Kumar, Rachel Bousfield, Johanna Nader, J. Wendi Bailey, Nicholas J. Beeching, Nicholas P. Day, Christopher M. Parry, William A Paxton
PLOS ONE.2015; 10(5): e0123719. CrossRef
Ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity
Makoto Matsubayashi, Fumiko Suzuta, Yoshimi Terayama, Kengo Shimojo, Takeshi Yui, Makoto Haritani, Tomoyuki Shibahara
Parasitology Research.2014; 113(8): 3023. CrossRef
The prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural Cambodian village
Fabian Schär, Tawin Inpankaew, Rebecca J. Traub, Virak Khieu, Anders Dalsgaard, Wissanuwat Chimnoi, Chamnan Chhoun, Daream Sok, Hanspeter Marti, Sinuon Muth, Peter Odermatt
Parasitology International.2014; 63(4): 597. CrossRef
Prevalence of Intestinal Parasitic Infections Among Rural Inhabitants of Hamadan City, Iran, 2012
Rasool Jafari, Mohammad Fallah, Hossein Yousofi Darani, Hosein Ali Yousefi, Mohammad Ali Mohaghegh, Milad Latifi, Mohammad Sadaghian, Amir Hossein Maghsood
Avicenna Journal of Clinical Microbiology and Infection.2014; 1(2): 21445. CrossRef
Hsp90 Inhibitors as New Leads To Target Parasitic Diarrheal Diseases
Anjan Debnath, Dea Shahinas, Clifford Bryant, Ken Hirata, Yukiko Miyamoto, Grace Hwang, Jiri Gut, Adam R. Renslo, Dylan R. Pillai, Lars Eckmann, Sharon L. Reed, James H. McKerrow
Antimicrobial Agents and Chemotherapy.2014; 58(7): 4138. CrossRef
Zoonoses in South-East Asia: a regional burden, a global threat
Marion Bordier, François Roger
Animal Health Research Reviews.2013; 14(1): 40. CrossRef
Prevalence of Intestinal Parasitic Infection in School Going Children in Amalapuram, Andhra Pradesh, India
Rangaiahagari Ashok, Giddi Suguneswari, Ksbvn Satish, Vedantham Kesavaram
Shiraz E-Medical Journal.2013;[Epub] CrossRef
Drugs for treating giardiasis
Carlos E Granados, Ludovic Reveiz, Luis G Uribe, Claudia P Criollo
Cochrane Database of Systematic Reviews.2012;[Epub] CrossRef
Genetic Hemoglobin Disorders, Infection, and Deficiencies of Iron and Vitamin A Determine Anemia in Young Cambodian Children
Joby George, Miriam Yiannakis, Barbara Main, Robyn Devenish, Courtney Anderson, Ung Sam An, Sheila M. Williams, Rosalind S. Gibson
The Journal of Nutrition.2012; 142(4): 781. CrossRef
Molecular Identification ofTaeniaTapeworms byCox1Gene in Koh Kong, Cambodia
Hyeong-Kyu Jeon, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Sung-Jong Hong, Eun-Taek Han, Hoo-Gn Jeong, Tep Chhakda, Muth Sinuon, Duong Socheat, Keeseon S. Eom
The Korean Journal of Parasitology.2011; 49(2): 195. CrossRef
Echinostoma revolutumInfection in Children, Pursat Province, Cambodia
Woon-Mok Sohn, Jong-Yil Chai, Tai-Soon Yong, Keeseon S. Eom, Cheong-Ha Yoon, Muth Sinuon, Duong Socheat, Soon-Hyung Lee
Emerging Infectious Diseases.2011; 17(1): 117. CrossRef
Epidemiology and control of human gastrointestinal parasites in children
Michael O Harhay, John Horton, Piero L Olliaro
Expert Review of Anti-infective Therapy.2010; 8(2): 219. CrossRef
Childhood Nephrotic Syndrome in Cambodia: An Association with Gastrointestinal Parasites
Lawrence Copelovitch, Orng Sam Ol, Sarah Taraquinio, Ngoun Chanpheaktra
The Journal of Pediatrics.2010; 156(1): 76. CrossRef
Prevalence of counterfeit anthelminthic medicines: a cross-sectional survey in Cambodia
Mohiuddin Hussain Khan, Junko Okumura, Tey Sovannarith, Nam Nivanna, Manabu Akazawa, Kazuko Kimura
Tropical Medicine & International Health.2010;[Epub] CrossRef
Reassessment of the epidemiology of amebiasis: State of the art
Cecilia Ximénez, Patricia Morán, Liliana Rojas, Alicia Valadez, Alejandro Gómez
Infection, Genetics and Evolution.2009; 9(6): 1023. CrossRef
Molecular epidemiology of amebiasis
Ibne Karim M. Ali, C. Graham Clark, William A. Petri
Infection, Genetics and Evolution.2008; 8(5): 698. CrossRef
The prevalence of intestinal parasites in the province of Izmir, Turkey
Hande Dagci, Özgür Kurt, Mete Demirel, Ipek Östan, Naser Reza Azizi, Aliye Mandiracioglu, Cengiz Yurdagül, Mehmet Tanyüksel, Ece Eroglu, Mucide Ak
Parasitology Research.2008; 103(4): 839. CrossRef
Prevalence of Giardia lamblia with or without diarrhea in South East, South East Asia and the Far East
Hassan H. Dib, Si Qi Lu, Shao Fang Wen
Parasitology Research.2008; 103(2): 239. CrossRef
Parasitic infestation and nutritional status among schoolchildren in Vientiane, Lao PDR
Outavong Phathammavong, Ali Moazzam, Duangphachan Xaysomphoo, Alongkone Phengsavanh, Chushi Kuroiwa
Journal of Paediatrics and Child Health.2007; 43(10): 689. CrossRef

8,631 View
111 Download
Crossref

Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica: Seobo Sim, Kyeong Ah Kim, Tai-Soon Yong, Soon-Jung Park, Kyung-il Im, Myeong Heon Shin; Korean J Parasitol 2004;42(4):205-208.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.205

Abstract
PDF

Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

Citations

Citations to this article as recorded by

Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica
Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin
The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
The state of art of neutrophil extracellular traps in protozoan and helminthic infections
César Díaz-Godínez, Julio C. Carrero
Bioscience Reports.2019;[Epub] CrossRef
Entamoeba histolytica Trophozoites Induce a Rapid Non-classical NETosis Mechanism Independent of NOX2-Derived Reactive Oxygen Species and PAD4 Activity
César Díaz-Godínez, Zayda Fonseca, Mario Néquiz, Juan P. Laclette, Carlos Rosales, Julio C. Carrero
Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
Entamoeba histolytica induces human neutrophils to form NETs
J. Ventura‐Juarez, MR. Campos‐Esparza, J. Pacheco‐Yepez, J. A López‐Blanco, A. Adabache‐Ortíz, M. Silva‐Briano, R. Campos‐Rodríguez
Parasite Immunology.2016; 38(8): 503. CrossRef
Modulatory mechanisms of enterocyte apoptosis by viral, bacterial and parasitic pathogens
Andre G Buret, Amol Bhargava
Critical Reviews in Microbiology.2014; 40(1): 1. CrossRef
Anoikis potential of Entameba histolytica secretory cysteine proteases: Evidence of contact independent host cell death
Sudeep Kumar, Rajdeep Banerjee, Nilay Nandi, Abul Hasan Sardar, Pradeep Das
Microbial Pathogenesis.2012; 52(1): 69. CrossRef
In vivo programmed cell death of Entamoeba histolytica trophozoites in a hamster model of amoebic liver abscess
J. D. Villalba-Magdaleno, G. Perez-Ishiwara, J. Serrano-Luna, V. Tsutsumi, M. Shibayama
Microbiology.2011; 157(5): 1489. CrossRef
TGF‐β‐regulated tyrosine phosphatases induce lymphocyte apoptosis in Leishmania donovani‐infected hamsters
Rajdeep Banerjee, Sudeep Kumar, Abhik Sen, Ananda Mookerjee, Syamal Roy, Subrata Pal, Pradeep Das
Immunology & Cell Biology.2011; 89(3): 466. CrossRef
Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica
Seobo Sim, Jae-Ran Yu, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2010; 48(4): 285. CrossRef
Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica
Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
International Journal for Parasitology.2007; 37(11): 1209. CrossRef
Involvement of β2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica
Seobo Sim, Soon-Jung Park, Tai-Soon Yong, Kyung-Il Im, Myeong Heon Shin
Microbes and Infection.2007; 9(11): 1368. CrossRef
Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase‐3 and reducing Mcl‐1 expression
J. H. KANG, H. O. SONG, J. S. RYU, M. H. SHIN, J. M. KIM, Y. S. CHO, J. F. ALDERETE, M. H. AHN, D. Y. MIN
Parasite Immunology.2006; 28(9): 439. CrossRef
Toxoplasma gondii Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation
Ji-Young Kim, Myoung-Hee Ahn, Hye-Sun Jun, Jai-Won Jung, Jae-Sook Ryu, Duk-Young Min
Yonsei Medical Journal.2006; 47(6): 862. CrossRef