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Volume 50(4); December 2012

Mini Review

Immunological Mechanisms by Which Concomitant Helminth Infections Predispose to the Development of Human Tuberculosis
Patricia M?ndez-Samperio
Korean J Parasitol 2012;50(4):281-286.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.281

Helminthic infections afflict over 1.5 billion people worldwide, while Mycobacterium tuberculosis infects one third of the world's population, resulting in 2 million deaths per year. Although tuberculosis and helminthic infections coexist in many parts of the world, and it has been demonstrated that the T-helper 2 and T-regulatory cell responses elicited by helminths can affect the ability of the host to control mycobacterial infection, it is still unclear whether helminth infections in fact affect tuberculosis disease. In this review article, current progress in the knowledge about the immunomodulation induced by helminths to diminish the protective immune responses to bacille Calmette-Guerin vaccination is reviewed, and the knowledge about the types of immune responses modulated by helminths and the consequences for tuberculosis are summarized. In addition, recent data supporting the significant reduction of both M. tuberculosis antigen-specific Toll-like receptor (TLR) 2 and TLR9 expression, and pro-inflammatory cytokine responses to TLR2 and TLR9 ligands in individuals with M. tuberculosis and helminth co-infection were discussed. This examination will allow to improve understanding of the immune responses to mycobacterial infection and also be of great relevance in combating human tuberculosis.

Citations

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    Shixiong He, Changjiao Luan, Jiaqi Gao, Feng Lu, Fang Tian, Muh Fauzi, She Zhang, Yinsong Chen, Xinlong He
    Microbial Pathogenesis.2026; 210: 108131.     CrossRef
  • Paradoxical Use of Benralizumab in Reactive Hypereosinophilia from Toxocariasis and Tuberculosis Co-Infection—Case Report and Literature Review
    Nicoleta Sorina Bertici, Talida Georgiana Cut, Amalia Ridichie, Andrei Raul Manzur, Razvan Adrian Bertici
    International Journal of Molecular Sciences.2025; 26(17): 8117.     CrossRef
  • Spatial associations of Hansen’s disease and schistosomiasis in endemic regions of Minas Gerais, Brazil
    Jessica L. Stephens, Lucia A. O. Fraga, José A. Ferreira, Laura De Mondesert, Uriel Kitron, Julie A. Clennon, Jessica K. Fairley, Georgios Pappas
    PLOS Neglected Tropical Diseases.2024; 18(12): e0012682.     CrossRef
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    Angela Guila
    Bulletin of the Academy of Sciences of Moldova. Medical Sciences.2024; 77(3): 239.     CrossRef
  • Clinical Analysis of the Renal Protective Effect of GLP-1 on Diabetic Patients Based on Edge Detection
    Jing Wang, Yang Wang, Ping Pang, Xiaomeng Jia, Xu Yan, Zhaohui Lv, Bhagyaveni M.A
    Journal of Healthcare Engineering.2022; 2022: 1.     CrossRef
  • Helminth infections and immunosenescence: The friend of my enemy
    Amir Abdoli, Hoda Mirzaian Ardakani
    Experimental Gerontology.2020; 133: 110852.     CrossRef
  • Fasciola hepatica coinfection modifies the morphological and immunological features of Echinococcus granulosus cysts in cattle
    Christian Hidalgo, Caroll Stoore, Marcela Hernández, Rodolfo Paredes
    Veterinary Research.2020;[Epub]     CrossRef
  • Intestinal parasites and HIV in Ethiopian tuberclosis patients: A systematic review and meta-analysis
    Getenet Dessie, Ayenew Negesse, Fasil Wagnew, Desalegne Amare, Balew Zeleke Tiruneh, Henok Mulugeta, Berhanu Abebaw Mekonen, Dessalegn Haile, Tilksew Ayalew, Tesfa Dejenie Habtewold
    Current Therapeutic Research.2020; 93: 100603.     CrossRef
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    Amir Abdoli, Mirza Ali Mofazzal Jahromi
    Archives of Medical Research.2019; 50(1): 19.     CrossRef
  • Seroprevalence and risk factors for Toxocara infection among children in a rural community in Fars province, southern Iran
    Bahador Sarkari, Roya Alirezaei, Akram Layegh Gigloo, Zahra Rezaei, Fataneh Mikaeili, Mohammad Saleh Bahreini, Mohammad Fararouei
    Parasite Immunology.2018;[Epub]     CrossRef
  • Design, Synthesis and Biological Evaluation of Some Triazole Schiff’s Base Derivatives as Potential Antitubercular Agents
    Asma A. Sager, Zainab S. Abood, Wedad M. El-Amary, Salah M. Bensaber, Inass A. Al-Sadawe, Nouri B. Ermeli, Salah B. Mohamed, Mohamed Al-Forgany, Ibrahim A. Mrema, Mabrouk Erhuma, Anton Hermann, Abdul M. Gbaj
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  • Parasitismo intestinal y tuberculosis
    Anuar Alonso Cedeño-Burbano, Ronal Fredy Cerón-Ortega, Carlos Eberth Pacichana-Agudelo, David Andrés Muñoz-García, Gerardo Alfonso Galeano-Triviño, Diana Catalina Cardona-Gómez, William Andrés Manquillo-Arias, Regina Victoria Plaza-Rivera
    Revista de la Facultad de Medicina.2017; 65(4): 673.     CrossRef
  • Molecular events by which dendritic cells promote Th2 immune protection in helmith infection
    Patricia Méndez-Samperio
    Infectious Diseases.2016; 48(10): 715.     CrossRef
  • Toll-like receptor signaling in parasitic infections
    Dalia S Ashour
    Expert Review of Clinical Immunology.2015; 11(6): 771.     CrossRef
  • Prevention of Type 1 Diabetes Through Parasite Infection
    Patricia Méndez-Samperio, Jorge Luis de-la-Rosa-Arana
    Immunotherapy.2015; 7(6): 595.     CrossRef
  • Co-infection of tuberculosis and parasitic diseases in humans: a systematic review
    Xin-Xu Li, Xiao-Nong Zhou
    Parasites & Vectors.2013;[Epub]     CrossRef
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  • 113 Download
  • Crossref

Original Articles

Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
Korean J Parasitol 2012;50(4):287-293.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.287

Knowledge of the prevalence of human Toxoplasma gondii infection is required in the Republic of Korea. In this study, we surveyed the seroprevalence of T. gondii infection and analyzed the risk factors associated with seropositivity among residents in 2 administrative districts; Seoul and the island of Jeju-do, which have contrasting epidemiologic characteristics. Sera and blood collected from 2,150 residents (1,114 in Seoul and 1,036 in Jeju-do) were checked for IgG antibody titers using ELISA and for the T. gondii B1 gene using PCR. In addition, participants completed a questionnaire that solicited information on gender, age, occupation, eating habits, history of contact with animals, and travel abroad. The T. gondii B1 gene was not detected in all residents examined. However, ELISA showed 8.0% (89 of 1,114 sera) positive for IgG antibodies against T. gondii in Seoul and 11.3% (117 of 1,036 sera) in Jeju-do. In both districts, the positive rates were higher in males than in females, and those 40-79 years of age showed higher rates than other ages. In Seoul, residents older than 70 years of age showed the highest positive rate, 14.9%, whereas in Jeju-do the highest prevalence, 15.6%, was in those in their sixties. The higher seropositive rate in Jeju-do than in Seoul may be related to eating habits and occupations. The present results and a review of related literature are indicative of an increased seroprevalence of T. gondii in Korea in recent years.

Citations

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  • Molecular detection of Toxoplasma gondii in wild oysters and seawater on the southeast coast of South Korea
    Hyejoo Shin, Sooji Hong, Seungwan Ryoo, Jong-Yil Chai, Bong-Kwang Jung
    Food Microbiology.2026; 134: 104925.     CrossRef
  • Clinical characteristics of toxoplasmosis patients in Korea: A retrospective study using health insurance review and assessment service data and electronic medical records
    Do-Won Ham, Bong-Kwang Jung, Ji-Hun Shin, Yong Joon Kim, Kyoung Yul Seo, Seung Mi Lee, Jae Hyoung Im, Jeong-Ran Kwon, Ho-Sung Lee, Kyung-Won Hwang, Eun-Hee Shin
    Parasites, Hosts and Diseases.2024; 62(4): 424.     CrossRef
  • Exosomal miRNA-21 from Toxoplasma gondii-infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes
    Bong-Kwang Jung, Hyemi Song, Hyejoo Shin, Jong-Yil Chai
    Scientific Reports.2022;[Epub]     CrossRef
  • Toxoplasmosis in pregnancy: a neglected bane but a serious threat in Nigeria
    Oyetunde T. Oyeyemi, Ifeoluwa T. Oyeyemi, Isaac A. Adesina, Adebisi M. Tiamiyu, Yinka D. Oluwafemi, Roseangela I. Nwuba, Rafaella F. Q. Grenfell
    Parasitology.2020; 147(2): 127.     CrossRef
  • Seroepidemiology and risk factors of toxoplasmosis among children age ranged from 1 to 14 years referred to medical diagnostic laboratories in Southeast Iran
    Vahid Raissi, Fatemeh Bayat, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim, Muhammad Getso, Ziba Hoseiny, Gita Alizadeh, Mehdi Khoshsima Shahraki, Soudabeh Etemadi
    Clinical Epidemiology and Global Health.2020; 8(2): 595.     CrossRef
  • Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep
    Céline Ducournau, Nathalie Moiré, Rodolphe Carpentier, Pauline Cantin, Clément Herkt, Isabelle Lantier, Didier Betbeder, Isabelle Dimier-Poisson
    Frontiers in Immunology.2020;[Epub]     CrossRef
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    Sun Huh
    Journal of the Korean Medical Association.2019; 62(8): 437.     CrossRef
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    Jeongjae Bak, Se-Hoon Shim, Young-Joon Kwon, Hwa-Young Lee, Ji Sun Kim, Heejung Yoon, Yeon Jung Lee
    Clinical Psychopharmacology and Neuroscience.2018; 16(1): 95.     CrossRef
  • TORCH (toxoplasmosis, rubella, cytomegalovirus, and herpes simplex virus) screening of small for gestational age and intrauterine growth restricted neonates: efficacy study in a single institute in Korea
    Mi Hae Chung, Chan Ok Shin, Juyoung Lee
    Korean Journal of Pediatrics.2018; 61(4): 114.     CrossRef
  • Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
    Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
    The Korean Journal of Parasitology.2018; 56(5): 437.     CrossRef
  • Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Cat Sitters in Korea
    Bong-Kwang Jung, Hyemi Song, Sang-Eun Lee, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(2): 203.     CrossRef
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    Infection & Chemotherapy.2016; 48(1): 54.     CrossRef
  • High Toxoplasma gondii Seropositivity among Brain Tumor Patients in Korea
    Bong-Kwang Jung, Hyemi Song, Min-Jae Kim, Jaeeun Cho, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2016; 54(2): 201.     CrossRef
  • Serological diagnosis of toxoplasmosis and standardization
    Kuo Zhang, Guigao Lin, Yanxi Han, Jinming Li
    Clinica Chimica Acta.2016; 461: 83.     CrossRef
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    Byung Gil Moon, Joo Yong Lee
    Hanyang Medical Reviews.2016; 36(3): 192.     CrossRef
  • Detection of Antibodies against Toxoplasma gondii in Cattle Raised in Gyeongbuk Province, Korea
    Jaejun Oh, Seung-Hun Lee, Seung-Jin Lee, Young-Hoan Kim, Seung-Chun Park, Man-Hee Rhee, Oh-Deog Kwon, Tae-Hwan Kim, Dongmi Kwak
    Journal of Food Protection.2016; 79(5): 821.     CrossRef
  • Prevalence ofToxoplasma gondiiandToxocara canisamong Patients with Uveitis
    Su Jin Lim, Sang Eun Lee, Sun Hyun Kim, Sung-Hee Hong, Yong Sung You, Oh Woong Kwon, Hyeun Seung Kim
    Ocular Immunology and Inflammation.2015; 23(2): 111.     CrossRef
  • Preliminary study on association between toxoplasmosis and breast cancer in Iran
    Narges Kalantari, Salman Ghaffari, Masomeh Bayani, Maryam Mitra Elmi, Daryush Moslemi, Novin Nikbakhsh, Fariedh Ghavipanjeh
    Asian Pacific Journal of Tropical Biomedicine.2015; 5(1): 44.     CrossRef
  • <i>Toxoplasma gondii</i> B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
    Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(3): 259.     CrossRef
  • Prevalence ofToxoplasma gondiiandToxocara canisamong Patients with Uveitis
    Su Jin Lim, Sang Eun Lee, Sun Hyun Kim, Sung-Hee Hong, Yong Sung You, Oh Woong Kwon, Hyeun Seung Kim
    Ocular Immunology and Inflammation.2014; 22(5): 360.     CrossRef
  • Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment
    Kyoung-Ho Pyo, Bong-Kwang Jung, Chun-Feng Xin, You-Won Lee, Jong-Yil Chai, Eun-Hee Shin
    The Korean Journal of Parasitology.2014; 52(6): 605.     CrossRef
  • Ocular toxoplasmosis past, present and new aspects of an old disease
    M. Maenz, D. Schlüter, O. Liesenfeld, G. Schares, U. Gross, U. Pleyer
    Progress in Retinal and Eye Research.2014; 39: 77.     CrossRef
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    Jaroslav Flegr, Madhukar Dama
    Folia Parasitologica.2014; 61(6): 485.     CrossRef
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    H. Bernin, H. Lotter
    Journal of Infectious Diseases.2014; 209(suppl 3): S107.     CrossRef
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    Jaroslav Flegr, Joseph Prandota, Michaela Sovičková, Zafar H. Israili, Delmiro Fernandez-Reyes
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    Sun Huh
    Journal of the Korean Medical Association.2013; 56(6): 513.     CrossRef
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Larval Anisakid Infections in Marine Fish from Three Sea Areas of the Republic of Korea
Shin-Hyeong Cho, Sang-Eun Lee, Ok-Hee Park, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2012;50(4):295-299.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.295

The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

Citations

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  • Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters
    Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn
    Parasitology Research.2024;[Epub]     CrossRef
  • Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
    Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
    EFSA Journal.2024;[Epub]     CrossRef
  • Parasites in the Skeletal Muscle of Amberjacks Seriola spp. in Japan: A Review
    Kazuo Ogawa
    Fish Pathology.2023; 58(4): 127.     CrossRef
  • Prevalence of Anisakid Nematodes in Fish in China: A Systematic Review and Meta-Analysis
    Qing Liu, Qi Wang, Jing Jiang, Jun-Yang Ma, Xing-Quan Zhu, Qing-Long Gong
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Epidemical study of third stage larvae of Anisakis spp. infection in marine fishes in China from 2016 to 2017
    Shuran Yang, Xiaoyan Pei, Ying Li, Li Zhan, Zhen Tang, Weiwei Chen, Xia Song, Dajin Yang
    Food Control.2020; 107: 106769.     CrossRef
  • Anisakidosis
    Heung Up Kim
    The Korean Journal of Helicobacter and Upper Gastrointestinal Research.2019; 19(1): 23.     CrossRef
  • <i>Anisakis pegreffii</i> Larvae in Sea Eels (<i>Astroconger myriaster</i>) from the South Sea, Republic of Korea
    Jaeeun Cho, Hyemi Lim, Bong-Kwang Jung, Eun-Hee Shin, Jong-Yil Chai
    The Korean Journal of Parasitology.2015; 53(3): 349.     CrossRef
  • Molecular Analysis ofAnisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea
    Woon-Mok Sohn, Jung-Mi Kang, Byoung-Kuk Na
    The Korean Journal of Parasitology.2014; 52(4): 383.     CrossRef
  • Clinical Update on Parasitic Diseases
    Min Seo
    Korean Journal of Medicine.2013; 85(5): 469.     CrossRef
  • 8,752 View
  • 90 Download
  • Crossref
Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-β and IL-4
Joon-Yong Chung, Young-An Bae, Doo-Hee Yun, Hyun-Jong Yang, Yoon Kong
Korean J Parasitol 2012;50(4):301-308.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.301

In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of CD19+ B cells was observed as early as week 1 post-infection while CD4+/CD8+ T cells were down-regulated. Accumulation of Mac1+ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-α mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-1β expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-β were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-β and IL-4 during the early stages of infection.

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    María Teresa Ruiz-Campillo, Isabel Lourdes Pacheco, Nieves Abril, María José Bautista, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Leandro Buffoni, José Pérez, Verónica Molina-Hernández, Rafael Zafra
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    Jae Seung Lee
    Clinical Ultrasound.2024; 9(1): 18.     CrossRef
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    Luis Miguel Flores-Velázquez, María Teresa Ruiz-Campillo, Guillem Herrera-Torres, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Rafael Zafra, Leandro Buffoni, Pablo José Rufino-Moya, Verónica Molina-Hernández, José Pérez
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    Clinical and Translational Discovery.2023;[Epub]     CrossRef
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    Pathogens.2020; 9(1): 46.     CrossRef
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    María Teresa Ruiz-Campillo, Verónica Molina-Hernández, María José Bautista, Isabel L. Pacheco, Rafael Zafra, Leandro Buffoni, Francisco Javier Martínez-Moreno, Alvaro Martínez-Moreno, José Pérez
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  • Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
    Isabel L. Pacheco, Nieves Abril, Rafael Zafra, Verónica Molina-Hernández, Noelia Morales-Prieto, María J. Bautista, María T. Ruiz-Campillo, Raúl Pérez-Caballero, Alvaro Martínez-Moreno, José Pérez
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    Claudia Machicado, Jorge D. Machicado, Vicente Maco, Angelica Terashima, Luis A. Marcos, Hector H Garcia
    PLOS Neglected Tropical Diseases.2016; 10(9): e0004962.     CrossRef
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  • 68 Download
  • Crossref
In Vitro Maintenance of Clonorchis sinensis Adult Worms
Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong
Korean J Parasitol 2012;50(4):309-315.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.309

Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke's solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke's solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke's solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke's solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke's solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.

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    Eun-Min Kim, Sung-Tae Hong
    Journal of Korean Medical Science.2025;[Epub]     CrossRef
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    Smita Sutrave, Martin Heinrich Richter
    Microorganisms.2023; 11(7): 1708.     CrossRef
  • In vitro culture and morphology of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) collected from the intestine of tambaqui (Colossoma macropomum) farmed in the Brazilian Amazon
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Foodborne Trematode Metacercariae in Fish from Northern Vietnam and Their Adults Recovered from Experimental Hamsters
Jong-Yil Chai, Nguyen Van De, Woon-Mok Sohn
Korean J Parasitol 2012;50(4):317-325.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.317

The prevalence of foodborne trematode (FBT) metacercariae was investigated in fish from 2 localities of northern Vietnam in 2004-2005. Freshwater fish (9 species) were collected from local markets in Hanoi City (n=76) and Nam Dinh Province (n=79), and were examined for FBT metacercariae using the artificial digestion technique. Adult flukes were obtained from hamsters experimentally infected with the metacercariae at day 8 post-infection. Three (Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium) and 6 (Haplorchis taichui, H. pumilio, C. formosanus, P. varium, Stellantchasmus falcatus, and Heterophyopsis continua) species of FBT metacercariae were detected in the 2 regions, respectively. Overall, among the positive fish species, H. pumilio metacercariae were detected in 104 (80.0%) of 130 fish examined (metacercarial density per infected fish; 64.2). C. formosanus metacercariae were found in 37 (40.2%) of 92 fish (metacercarial density; 14.7). P. varium metacercariae were detected in 19 (63.3%) of 30 fish (Anabas testudineus and Mugil cephalus) (metacercarial density; 247.7). S. falcatus metacercariae were found in all 10 M. cephalus examined (metacercarial density; 84.4). H. continua metacercariae (2 in number) were detected in 1 fish of Coilia lindmani. Morphologic characteristics of the FBT metacercariae and their experimentally obtained adults were described. The results have demonstrated that various FBT species are prevalent in northen parts of Vietnam.

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  • The prevalence of cercarial infection and development of a duplex PCR for detection of the cercarial stage of Haplorchis taichui and H. pumilio in first intermediate hosts from Chai Nat province, Thailand
    Chadaporn Dunghungzin, Thapana Chontananarth
    Acta Tropica.2021; 214: 105795.     CrossRef
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    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
  • High Prevalence of Clonorchis sinensis and Other Zoonotic Trematode Metacercariae in Fish from a Local Market in Yen Bai Province, Northern Vietnam
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    The Korean Journal of Parasitology.2020; 58(3): 333.     CrossRef
  • Larval Gnathostomes and Zoonotic Trematode Metacercariae in Fish from a Local Market in Yangon City, Myanmar
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    Parasites & Vectors.2017;[Epub]     CrossRef
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    The Korean Journal of Parasitology.2017; 55(6): 631.     CrossRef
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    The Korean Journal of Parasitology.2016; 54(4): 497.     CrossRef
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    The Korean Journal of Parasitology.2016; 54(4): 537.     CrossRef
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    The Korean Journal of Parasitology.2013; 51(6): 719.     CrossRef
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Monthly Occurrence of Vectors and Reservoir Rodents of Scrub Typhus in an Endemic Area of Jeollanam-do, Korea
Seung Hyun Lee, Young-Sun Lee, In Yong Lee, Jae Won Lim, Hee-Kwan Shin, Jae-Ran Yu, Seobo Sim
Korean J Parasitol 2012;50(4):327-331.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.327

Monthly surveys were conducted to investigate the occurrence of chigger mites and seroprevalence of scrub typhus among small mammals in Jeollanam-do, the southwestern part of Korea, from November 2006 through October 2007. Fifty-eight small mammals, including 57 Apodemus agrarius (98.3%) and 1 Crocidura lasiura (1.7%), were captured, and a total of 4,675 chigger mites representing 4 genera and 8 species were collected from them. The chigger infestation rate among small mammals was 69.0%. The most predominant species in A. agrarius was Leptotrombidium scutellare (54.0%), followed by Leptotrombidium pallidum (39.4%), Leptotrombidium orientale (4.4%), Leptotrombidium palpale (1.1%), Neotrombicula tamiyai (0.6%), Eushoengastia koreaensis (0.3%), Neotrombicula gardellai (0.3%), and Cheladonta ikaoensis (<0.1%). The chigger index of A. agrarius was the highest in October (740.0), followed by November (242.0), September (134.6), March (98.3), February (38.2), January (35.3), December (34.5), April (30.8), and May (1.7). The average antibody positive rate of scrub typhus in wild rodents was 50.0%. The seropositive rates were high in October (100.0%) and November (83.3%), whereas those in other months were relatively low (28.6-57.1%). The chigger index of L. scutellare rapidly increased in September to form an acuminate peak in October, followed by a gradual decline. These results suggest that the outbreak of scrub typhus in the southwestern part of Korean peninsula is mostly due to L. scutellare.

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Case Reports

An Indigenous Case of Intestinal Capillariasis with Protein-Losing Enteropathy in Korea
Woon Tae Jung, Hyun Jin Kim, Hyun Ju Min, Chang Yoon Ha, Hong Jun Kim, Gyung Hyuck Ko, Byoung-Kuk Na, Woon-Mok Sohn
Korean J Parasitol 2012;50(4):333-337.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.333

We encountered an indigenous case of intestinal capillariasis with protein-losing enteropathy in the Republic of Korea. A 37-year-old man, residing in Sacheon-si, Gyeongsangnam-do, admitted to the Gyeongsang National University Hospital (GNUH) due to long-lasting diarrhea, abdominal pain, anasarca, and weight loss. He recalled that he frequently ate raw fish, especially the common blackish goby (Acanthogobius flavimanus) and has never been abroad. Under the suspicion of protein-losing enteropathy, he received various kinds of medical examinations, and was diagnosed as intestinal capillariasis based on characteristic sectional findings of nematode worms in the biopsied small intestine. Adults, juvenile worms, and eggs were also detected in the diarrheic stools collected before and after medication. The clinical symptoms became much better after treatment with albendazole 400 mg daily for 3 days, and all findings were in normal range in laboratory examinations performed after 1 month. The present study is the 6th Korean case of intestinal capillariasis and the 3rd indigenous one in the Republic of Korea.

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An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam
Nguyen Van De, Nguyen Vu Trung, Nguyen Hong Ha, Vu Thi Nga, Nguyen Minh Ha, Pham Thanh Thuy, Le Van Duyet, Jong-Yil Chai
Korean J Parasitol 2012;50(4):339-343.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.339

The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.

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  • An overview of human helminthioses in Vietnam: Their prevention, control and lessons learnt
    Hung Manh Nguyen, Dung Trung Do, Stephen E. Greiman, Ha Van Nguyen, Hien Van Hoang, Toan Quoc Phan, Phuc Pham-Duc, Henry Madsen
    Acta Tropica.2023; 238: 106753.     CrossRef
  • Food safety incidents in the red meat industry: A review of foodborne disease outbreaks linked to the consumption of red meat and its products, 1991 to 2021
    Dein Warmate, Bukola A. Onarinde
    International Journal of Food Microbiology.2023; 398: 110240.     CrossRef
  • Skewness in the literature on infectious livestock diseases in an emerging economy – the case of Vietnam
    Elisabeth Rajala, Hu Suk Lee, Nguyen Hoai Nam, Chu Thị Thanh Huong, Hoang Minh Son, Barbara Wieland, Ulf Magnusson
    Animal Health Research Reviews.2021; 22(1): 1.     CrossRef
  • Review of Biological and Chemical Health Risks Associated with Pork Consumption in Vietnam: Major Pathogens and Hazards Identified in Southeast Asia
    Meghan A. Cook, Pham Duc Phuc
    Journal of Food Quality.2019; 2019: 1.     CrossRef
  • Surveillance and diagnosis of zoonotic foodborne parasites
    Reza Zolfaghari Emameh, Sami Purmonen, Antti Sukura, Seppo Parkkila
    Food Science & Nutrition.2018; 6(1): 3.     CrossRef
  • A systematic review of taeniasis, cysticercosis and trichinellosis in Vietnam
    Dinh Ng-Nguyen, Mark A. Stevenson, Rebecca J. Traub
    Parasites & Vectors.2017;[Epub]     CrossRef
  • Human Trichinosis in rural area Mountainous Provinces in Vietnam in 2015-2016
    Thu Huong Nguyen
    Global Journal of Infectious Diseases and Clinical Research.2017; : 009.     CrossRef
  • Innovative molecular diagnosis of Trichinella species based on β‐carbonic anhydrase genomic sequence
    Reza Zolfaghari Emameh, Marianne Kuuslahti, Anu Näreaho, Antti Sukura, Seppo Parkkila
    Microbial Biotechnology.2016; 9(2): 172.     CrossRef
  • Molecular differentiation of Trichinella spiralis, T. pseudospiralis, T. papuae and T. zimbabwensis by pyrosequencing
    L. Sadaow, C. Tantrawatpan, P.M. Intapan, V. Lulitanond, T. Boonmars, N. Morakote, E. Pozio, W. Maleewong
    Journal of Helminthology.2015; 89(1): 118.     CrossRef
  • Prevalence of Endemic Pig-Associated Zoonoses in Southeast Asia: A Review of Findings from the Lao People's Democratic Republic
    Anna L. Okello, Stephanie Burniston, James V. Conlan, Phouth Inthavong, Boualam Khamlome, Susan C. Welburn, Jeffrey Gilbert, John Allen, Stuart D. Blacksell
    The American Journal of Tropical Medicine and Hygiene.2015; 92(5): 1059.     CrossRef
  • Trichinellosis in Vietnam
    Vu Thi Nga, Nguyen Van De, Pham Ngoc Minh, Nguyen Vu Trung, Edoardo Pozio, Do Trung Dung, Pierre Dorny
    The American Journal of Tropical Medicine and Hygiene.2015; 92(6): 1265.     CrossRef
  • Trichinella infection in wild boars and synanthropic rats in northwest Vietnam
    N. Vu Thi, V.D. Nguyen, N. Praet, L. Claes, S. Gabriël, N.T. Huyen, P. Dorny
    Veterinary Parasitology.2014; 200(1-2): 207.     CrossRef
  • The Hidden Burden of Trichinellosis in Vietnam: A Postoutbreak Epidemiological Study
    Nga Vu Thi, Dung Do Trung, Amber Litzroth, Nicolas Praet, Huong Nguyen Thu, Hien Nguyen Thu, Hung Nguyen Manh, Pierre Dorny
    BioMed Research International.2013; 2013: 1.     CrossRef
  • 9,447 View
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Paragonimiasis in the Abdominal Cavity and Subcutaneous Tissue: Report of 3 Cases
Chang Ho Lee, Jong Hun Kim, Woo Sung Moon, Min Ro Lee
Korean J Parasitol 2012;50(4):345-347.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.345

Paragonimiasis is a parasitic disease caused by the lung fluke, Paragonimus spp. Lung flukes may be found in various organs, such as the brain, peritoneum, subcutaneous tissues, and retroperitoneum, other than the lungs. Abdominal paragonimiasis raises a considerable diagnostic challenge to clinicians, because it is uncommon and may be confused with other abdominopelvic inflammatory diseases, particularly peritoneal tuberculosis, and peritoneal carcinomatosis. Also, subcutaneous paragonimiasis does not easily bring up clinical suspicion, due to its rarity. We herein report 2 cases of abdominal paragonimiasis and 1 case of subcutaneous paragonimiasis in Korea.

Citations

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  • Survival and infectivity of Paragonimus westermani Metacercariae in soy sauce–marinated crayfish
    Eun-Min Kim, Yan Jin, Sung-Tae Hong
    Food and Waterborne Parasitology.2025; 40: e00277.     CrossRef
  • Pulmonary, liver and cerebral paragonimiasis: An unusual clinical case in Colombia
    Angel O. Donato-R., Jhon C. Donato-R.
    Travel Medicine and Infectious Disease.2022; 46: 102253.     CrossRef
  • Ectopic peritoneal paragonimiasis mimicking tuberculous peritonitis: A care report
    Jung Woo Choi, Chang Min Lee, Seong Je Kim, Se In Hah, Ji Yoon Kwak, Hyun Chin Cho, Chang Yoon Ha, Woon Tae Jung, Ok Jae Lee
    World Journal of Clinical Cases.2022; 10(16): 5359.     CrossRef
  • Intraspinal Paragonimiasis in Children: MRI Findings and Suggestions for Pathogenesis
    Y. Qin, J. Cai, W. Ji, X. Chen, L. Tian, S. Jun, L. Wang, X. He
    American Journal of Neuroradiology.2019;[Epub]     CrossRef
  • Simultaneous Paragonimus infection involving the breast and lung: A case report
    Moon Young Oh, Ajung Chu, Jeong Hwan Park, Jong Yoon Lee, Eun Youn Roh, Young Jun Chai, Ki-Tae Hwang
    World Journal of Clinical Cases.2019; 7(24): 4292.     CrossRef
  • Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs
    Tae Im Kim, Se-Ra Oh, Fuhong Dai, Hyun-Jong Yang, Sang-Do Ha, Sung-Jong Hong
    Parasitology Research.2017; 116(3): 1003.     CrossRef
  • Paragonimiasis in Children in Southwest China
    Zongrong Gong, Ruixue Miao, Min Shu, Yu Zhu, Yang Wen, Qin Guo, Qiong Liao, Chaomin Wan
    Medicine.2017; 96(25): e7265.     CrossRef
  • Recent Incidence of Paragonimus westermani Metacercariae in Freshwater Crayfish, Cambaroides similis, from Two Enzootic Sites in Jeollanam-do, Korea
    Jin-Ho Song, Fuhong Dai, Xuelian Bai, Tae-Im Kim, Hyun-Jong Yang, Tong-Soo Kim, Shin-Hyung Cho, Sung-Jong Hong
    The Korean Journal of Parasitology.2017; 55(3): 347.     CrossRef
  • A Case of Ectopic Peritoneal Paragonimiasis Mimicking Diverticulitis or Abdominal Abscess
    Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Yong Sik Yoon, Kyung Won Kim, Jaeeun Cho, Jong-Yil Chai, Yong Pil Chong
    The Korean Journal of Parasitology.2017; 55(3): 313.     CrossRef
  • Hepatic paragonimiasis in a 15-month-old girl: a case report
    Zongrong Gong, Zhicheng Xu, Chuanfen Lei, Chaomin Wan
    BMC Pediatrics.2017;[Epub]     CrossRef
  • Distinct MDCT imaging features to differential diagnosis of hepatic paragonimiasis and small hepatocellular carcinoma
    Sheng Zhang, Si-Ming Xie, Yong-Hua Chen, Xu-Bao Liu, Gang Mai
    Oncotarget.2017; 8(23): 37291.     CrossRef
  • A Case of Intra-abdominal Paragonimiasis Mimicking Metastasis of Lung Cancer Diagnosed by Endoscopic Ultrasound-guided Fine Needle Aspiration
    Cho Rong Oh, Mi-Jin Kim, Kwang Hyuck Lee
    The Korean Journal of Gastroenterology.2015; 66(1): 41.     CrossRef
  • Pancreatic paragonimiasis mimics pancreatic cystic-solid tumor – A case report
    Xiaodong Yang, Mingqing Xu, Yang Wu, Bo Xiang
    Pancreatology.2015; 15(5): 576.     CrossRef
  • Cutaneous paragonimiasis due to triploid Paragonimus westermani presenting as a non-migratory subcutaneous nodule: a case report
    Makoto Kodama, Mayumi Akaki, Hiroyuki Tanaka, Haruhiko Maruyama, Eiji Nagayasu, Tetsuhiro Yokouchi, Yasuji Arimura, Hiroaki Kataoka
    Journal of Medical Case Reports.2014;[Epub]     CrossRef
  • Clinical Update on Parasitic Diseases
    Min Seo
    Korean Journal of Medicine.2013; 85(5): 469.     CrossRef
  • Paragonimiasis: A Pictorial Essay
    Kyung Nyeo Jeon, Mi Jung Park, Kyungsoo Bae, Hae Young Choi, Ho Cheol Choi, Jae Boem Na, Dae Seob Choi, Ho Cheol Kim, In Seok Jang, Dong Chul Kim
    Journal of the Korean Society of Radiology.2013; 69(5): 365.     CrossRef
  • 8,766 View
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Intestinal Paragonimiasis with Colonic Ulcer and Hematochezia in An Elderly Taiwanese Woman
Chung-Te Liu, Yen-Cheng Chen, Tso-Hsiao Chen, Ursula Barghouth, Chia-Kwung Fan
Korean J Parasitol 2012;50(4):349-352.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.349

A 94-year-old female with end-stage renal disease presents with fever, fatigue, and hematochezia. She had previously resided in Hunan Province, China, and Myanmar, and she immigrated to Taiwan 30 years ago. Colonoscopy revealed a colonic ulcer. Biopsy of the colonic ulcer showed ulceration of the colonic mucosa, and many Paragonimus westermani-like eggs were noted. Serum IgG antibody levels showed strong reactivity with P. westermani excretory-secretory antigens by ELISA. Intestinal paragonimiasis was thus diagnosed according to the morphology of the eggs and serologic finding. After treatment with praziquantel, hematochezia resolved. The present case illustrates the extreme manifestations encountered in severe intestinal paragonimiasis.

Citations

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  • Incidental diagnosis of paragonimiasis after histopathological examination of cholecystectomy specimen in Nepal: A case report
    Pearlbiga Karki, Pinky Jha, Gaurab Mainali, Manoj Khadka, Prabesh Karki, Jung Bahadur Thapa, Gayatri Karki
    Annals of Medicine and Surgery.2021; 63: 102170.     CrossRef
  • A Case of Ectopic Peritoneal Paragonimiasis Mimicking Diverticulitis or Abdominal Abscess
    Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Yong Sik Yoon, Kyung Won Kim, Jaeeun Cho, Jong-Yil Chai, Yong Pil Chong
    The Korean Journal of Parasitology.2017; 55(3): 313.     CrossRef
  • Current status of Paragonimus and paragonimiasis in Ecuador
    Manuel Calvopiña, Daniel Romero, Byron Castañeda, Yoshihisa Hashiguchi, Hiromu Sugiyama
    Memórias do Instituto Oswaldo Cruz.2014; 109(7): 849.     CrossRef
  • 10,249 View
  • 76 Download
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A Case of Inguinal Sparganosis Mimicking Myeloid Sarcoma
Jin Yeob Yeo, Jee Young Han, Jung Hwan Lee, Young Hoon Park, Joo Han Lim, Moon Hee Lee, Chul Soo Kim, Hyeon Gyu Yi
Korean J Parasitol 2012;50(4):353-355.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.353

We report here a case of inguinal sparganosis, initially regarded as myeloid sarcoma, diagnosed in a patient undergone allogeneic hematopoietic transplantation (HSCT). A 56-year-old male patient having myelodysplastic syndrome was treated with allogeneic HSCT after myeloablative conditioning regimen. At day 5 post-HSCT, the patient complained of a painless palpable mass on the left scrotum and inguinal area. Pelvic magnetic resonance imaging and computed tomography revealed suspected myeloid sarcoma. Gun-biopsy was performed, and the result revealed eosinophilic infiltrations without malignancy. Subsequent serologic IgG antibody test was positive for sparganum. Excisional biopsy as a therapeutic diagnosis was done, and the diagnosis of sparganosis was confirmed eventually. This is the first report of sparganosis after allogeneic HSCT mimicking myeloid sarcoma, giving a lesson that the physicians have to consider the possibility of sparganosis in this clinical situation and perform adequate diagnostic and therapeutic approaches.

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  • Human Sparganosis in Korea
    Jeong-Geun Kim, Chun-Seob Ahn, Woon-Mok Sohn, Yukifumi Nawa, Yoon Kong
    Journal of Korean Medical Science.2018;[Epub]     CrossRef
  • Scrotal Sparganosis Mimicking Scrotal Teratoma in an Infant: A Case Report and Literature Review
    Yi-Ming Zhao, Hao-Chuan Zhang, Zhong-Rong Li, Hai-Yan Zhang
    The Korean Journal of Parasitology.2014; 52(5): 545.     CrossRef
  • 9,098 View
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An Imported Case of Cystic Echinococcosis in the Liver
Keun Soo Ahn, Sung-Tae Hong, Yu Na Kang, Jung Hyeok Kwon, Mi Jeong Kim, Tae Jun Park, Yong Hoon Kim, Tae Jin Lim, Koo Jeong Kang
Korean J Parasitol 2012;50(4):357-360.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.357

A 25-year-old Uzbek male presented with right upper abdominal pain for 20 days. On radiologic studies, a huge cystic mass was noticed in the right liver which was suspected as parasitic. The patient received right hepatic segmentectomy (segment 7), and the surgically resected mass was confirmed as cystic echinococcosis (CE), measuring 10.5 cm in its diameter. The inner surface of the cyst was bile-stained. The patient was discharged on the 8th hospital day, and was rechecked 6 months after the surgical intervention without any evidence of recurrence. The present report describes findings of an imported case of CE which represented ultrasound images of the 'ball of wool'.

Citations

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  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Delayed Diagnosis of Imported Cystic Echinococcosis and Successful Treatment With Percutaneous Drainage and Albendazole in Korea: A Case Report
    Won Jun Choi, Hanna Jin, Hyeon Jae Jo, Chan Mi Lee, Chang Kyung Kang, Pyoeng Gyun Choe, Wan Beom Park, Nam Joong Kim, Min-Ho Choi
    Journal of Korean Medical Science.2025;[Epub]     CrossRef
  • Hepatic Hydatid Cyst: A Case Report
    Wan Chul Kim, Jae Uk Shin, Su Sin Jin
    The Korean Journal of Gastroenterology.2021; 77(1): 35.     CrossRef
  • An Imported Case of Disseminated Echinococcosis in Korea
    Dong Hoon Shin, Hae Chan Jo, Jeong-Han Kim, Kang Il Jun, Wan Beom Park, Nam-Joong Kim, Min-Ho Choi, Chang Kyung Kang, Myoung-don Oh
    The Korean Journal of Parasitology.2019; 57(4): 429.     CrossRef
  • The echinococcoses in Asia: The present situation
    Akira Ito, Christine M. Budke
    Acta Tropica.2017; 176: 11.     CrossRef
  • Medical treatment of tropical parasitic diseases
    Joon Sup Yeom, Yoon Kong
    Journal of the Korean Medical Association.2016; 59(9): 721.     CrossRef
  • Primary Renal Hydatid Cyst: Mis-Interpretation as a Renal Malignancy
    Hoon Choi, Jae Young Park, Jae-Heon Kim, Du Geon Moon, Jeong-Gu Lee, Jae Hyun Bae
    The Korean Journal of Parasitology.2014; 52(3): 295.     CrossRef
  • Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis
    Sabariah Osman, Akbar Khalilpour, Rahmah Noordin, Seyed Mahmoud Sadjjadi, Muhammad Hafiznur Yunus, Zohreh Kazemi Moghadam, Nor Dyana Zakaria
    The American Journal of Tropical Medicine and Hygiene.2014; 91(5): 994.     CrossRef
  • Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep
    Yan Jin, Khikmat Anvarov, Abdukhakim Khajibaev, Samin Hong, Sung-Tae Hong
    The Korean Journal of Parasitology.2013; 51(3): 313.     CrossRef
  • Clinical Update on Parasitic Diseases
    Min Seo
    Korean Journal of Medicine.2013; 85(5): 469.     CrossRef
  • Infection Status of Hydatid Cysts in Humans and Sheep in Uzbekistan
    Sung-Tae Hong, Yan Jin, Khikmat Anvarov, Abdukhakim Khadjibaev, Samin Hong, Yusufjon Ahmedov, Utkir Otaboev
    The Korean Journal of Parasitology.2013; 51(3): 383.     CrossRef
  • 9,559 View
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Brief Communications
Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba
Eun-Kyung Moon, Hyun-Hee Kong
Korean J Parasitol 2012;50(4):361-364.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.361

The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose is one of the primary components of the inner cyst wall, cellulose synthesis is essential to the process of cyst formation in Acanthamoeba. In this study, we hypothesized the key and short-step process in synthesis of cellulose from glycogen in encysting Acanthamoeba castellanii, and confirmed it by comparing the expression pattern of enzymes involving glycogenolysis and cellulose synthesis. The genes of 3 enzymes, glycogen phosphorylase, UDP-glucose pyrophosphorylase, and cellulose synthase, which are involved in the cellulose synthesis, were expressed high at the 1st and 2nd day of encystation. However, the phosphoglucomutase that facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate expressed low during encystation. This report identified the short-cut pathway of cellulose synthesis required for construction of the cyst wall during the encystation process in Acanthamoeba. This study provides important information to understand cyst wall formation in encysting Acanthamoeba.

Citations

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  • Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
    Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
    Pathogens.2025; 14(3): 268.     CrossRef
  • Inhibition of GABA metabolism by β-lactam antibiotics affects encystation in Acanthamoeba
    Chih-Ming Tsai, Yao-Tsung Chang, Yu-Jen Wang, Chun-Hsien Chen, Chuan-Yi Wang, Jian-Ming Huang
    Biomedicine & Pharmacotherapy.2025; 193: 118841.     CrossRef
  • Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
    Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
    Parasitology Research.2024;[Epub]     CrossRef
  • Biological characteristics and pathogenicity of Acanthamoeba
    Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
    Rachasak Boonhok, Suthinee Sangkanu, Suganya Phumjan, Ramita Jongboonjua, Nawarat Sangnopparat, Pattamaporn Kwankaew, Aman Tedasen, Chooi Ling Lim, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Alok
    PeerJ.2022; 10: e13657.     CrossRef
  • Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
    Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
    Frontiers in Cell and Developmental Biology.2022;[Epub]     CrossRef
  • Mechanisms of Effector-Mediated Immunity Revealed by the Accidental Human Pathogen Legionella pneumophila
    Tshegofatso Ngwaga, Deepika Chauhan, Stephanie R. Shames
    Frontiers in Cellular and Infection Microbiology.2021;[Epub]     CrossRef
  • Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
    Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
    Pathogens.2021; 10(7): 842.     CrossRef
  • Evolution and function of carbohydrate reserve biosynthesis in parasitic protists
    Julie E. Ralton, M. Fleur Sernee, Malcolm J. McConville
    Trends in Parasitology.2021; 37(11): 988.     CrossRef
  • The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
    So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
    Parasites & Vectors.2020;[Epub]     CrossRef
  • Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
    Pauline Schaap, Christina Schilde
    Microbiology.2018; 164(5): 727.     CrossRef
  • Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
    Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
    The Korean Journal of Parasitology.2014; 52(2): 131.     CrossRef
  • 10,034 View
  • 100 Download
  • Crossref
Effects of Mannose on Pathogenesis of Acanthamoeba castellanii
Kyung-Tae Yoo, Suk-Yul Jung
Korean J Parasitol 2012;50(4):365-369.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.365

Acanthamoeba spp. are single-celled protozoan organisms that are widely distributed in the environment. In this study, to understand functional roles of a mannose-binding protein (MBP), Acanthamoeba castellanii was treated with methyl-alpha-D-mannopyranoside (mannose), and adhesion and cytotoxicity of the amoeba were analyzed. In addition, to understand the association of MBP for amoeba phagocytosis, phagocytosis assay was analyzed using non-pathogenic bacterium, Escherichia coli K12. Amoebae treated with mannose for 20 cycles exhibited larger vacuoles occupying the most area of the amoebic cytoplasm in comparison with the control group amoebae and glucose-treated amoebae. Mannose-selected amoebae exhibited lower levels of binding to Chinese hamster ovary (CHO) cells. Exogenous mannose inhibited >50% inhibition of amoebae (control group) binding to CHO cells. Moreover, exogenous mannose inhibited amoebae (i.e., man-treated) binding to CHO cells by <15%. Mannose-selected amoebae exhibited significantly decreased cytotoxicity to CHO cells compared with the control group amoebae, 25.1% vs 92.1%. In phagocytic assay, mannose-selected amoebae exhibited significant decreases in bacterial uptake in comparison with the control group, 0.019% vs 0.03% (P<0.05). Taken together, it is suggested that mannose-selected A. castellanii trophozoites should be severely damaged and do not well interact with a target cell via a lectin of MBP.

Citations

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  • Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
    Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
    Pathogens.2024; 13(2): 142.     CrossRef
  • Biological characteristics and pathogenicity of Acanthamoeba
    Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Commensals Serve as Natural Barriers to Mammalian Cells during Acanthamoeba castellanii Invasion
    Yu-Jen Wang, Chun-Hsien Chen, Jenn-Wei Chen, Wei-Chen Lin, Kevin R. Theis
    Microbiology Spectrum.2021;[Epub]     CrossRef
  • Lectins as Virulence Factors in Entamoeba Histolytica and Free-Living Amoebae
    Paula Guzmán-Téllez, Moisés Martínez-Castillo, Nadia Flores-Huerta, Gabriela Rosales-Morgan, Judith Pacheco-Yépez, Mireya de la Garza, Jesús Serrano-Luna, Mineko Shibayama
    Future Microbiology.2020; 15(10): 919.     CrossRef
  • Acanthamoeba castellanii as an alternative interaction model for the dermatophyte Trichophyton rubrum
    Lucas V. de Faria, Paulo H. F. do Carmo, Marliete C. da Costa, Nalu T. A. Peres, Isabela A. Rodrigues Chagas, Cinthia Furst, Gabriella F. Ferreira, Adriana O. Costa, Daniel A. Santos
    Mycoses.2020; 63(12): 1331.     CrossRef
  • Interactions Between Acanthamoeba culbertsoni and Pathogenic Bacteria and their Inhibition by Lectin-Antibodies
    Suk-Yul Jung
    Journal of Pure and Applied Microbiology.2020; 14(3): 1687.     CrossRef
  • Tupanvirus-infected amoebas are induced to aggregate with uninfected cells promoting viral dissemination
    Graziele Oliveira, Lorena Silva, Thiago Leão, Said Mougari, Flávio Guimarães da Fonseca, Erna Geessien Kroon, Bernard La Scola, Jônatas Santos Abrahão
    Scientific Reports.2019;[Epub]     CrossRef
  • Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization
    A-Young Kang, A-Young Park, Ho-Joon Shin, Naveed Ahmed Khan, Sutherland K. Maciver, Suk-Yul Jung
    Experimental Parasitology.2018; 192: 19.     CrossRef
  • Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression
    Sook-Luan Ng, Anisah Nordin, Norzana Abd Ghafar, Yusof Suboh, Noraina Ab Rahim, Kien-Hui Chua
    Parasites & Vectors.2017;[Epub]     CrossRef
  • 8,351 View
  • 103 Download
  • Crossref
Evaluation of Anti-Toxoplasma IgG, IgM, and IgA in Mothers with Spontaneous Abortion in Zanjan, Northwest Iran
Abbas Amin, S. Mazloomzadeh, A. Haniloo, F. Mohammadian, Asghar Fazaeli
Korean J Parasitol 2012;50(4):371-374.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.371

Toxoplasma gondii is one of the major agents of infectious abortions and due to its worldwide distribution can threat healthy pregnant women who had no previous exposure to this parasite. The present study was designed to investigate the contribution of T. gondii to spontaneous abortions in Zanjan, Northwest of Iran, using ELISA method. Blood Samples were collected from 264 mothers referred to the provincial hospitals of Zanjan due to spontaneous abortion. The sera were isolated and subjected to evaluate the anti-Toxoplasma IgG, IgM and IgA antibodies. The results showed IgG positive (IgG+) in 99 cases (37.5%). A total of 68 women (25.8%) showed seroconversion with IgM or IgA or both IgM and IgA. They included: IgM+ in 21 (8.0%), IgA+ in 23 (8.7%) and both IgM+ and IgA+ in 24 (9.1%) subjects. In 23 cases, positive titers of IgM and IgG were accompanied. In general, the analysis of anti-Toxoplasma antibody patterns, showed that about 17% of the spontaneous abortions were associated with serological patterns of acute infection. According to these findings, a considerable proportion of spontaneous abortions can be attributed to T. gondii in the study area.

Citations

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  • Screening for the Detection of Toxoplasma gondii IgG, IgM and IgA in Females of Reproductive Age from Western Romania
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Oxidative Stress in Vivax Malaria
Ramazan Bilgin, Mustafa S. Yalcin, Guzide Yucebilgic, Ismail S. Koltas, S?leyman Yazar
Korean J Parasitol 2012;50(4):375-377.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.375

Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P. vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were 8.07±2.29 nM/ml, 2.69±0.33 U/ml, and 49.6±3.2 U/g Hb in the patient group and 2.72±0.50 nM/ml, 3.71±0.47 U/ml, and 62.3±4.3 U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P<0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be significantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P. vivax.

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Prevalence of Drug Resistance-Associated Gene Mutations in Plasmodium vivax in Central China
Feng Lu, Bo Wang, Jun Cao, Jetsumon Sattabongkot, Huayun Zhou, Guoding Zhu, Kwonkee Kim, Qi Gao, Eun-Taek Han
Korean J Parasitol 2012;50(4):379-384.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.379

Resistance of Plasmodium spp. to anti-malarial drugs is the primary obstacle in the fight against malaria, and molecular markers for the drug resistance have been applied as an adjunct in the surveillance of the resistance. In this study, we investigated the prevalence of mutations in pvmdr1, pvcrt-o, pvdhfr, and pvdhps genes in temperate-zone P. vivax parasites from central China. A total of 26 isolates were selected, including 8 which were previously shown to have a lower susceptibility to chloroquine in vitro. For pvmdr1, pvcrt-o, and pvdhps genes, no resistance-conferring mutations were discovered. However, a highly prevalent (69.2%), single-point mutation (S117N) was found in pvdhfr gene. In addition, tandem repeat polymorphisms existed in pvdhfr and pvdhps genes, which warranted further studies in relation to the parasite resistance to antifolate drugs. The study further suggests that P. vivax populations in central China may still be relatively susceptible to chloroquine and sulfadoxine-pyrimethamine.

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    Monika Matlani, Amit Kumar, Vineeta Singh
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    Marcelo U. Ferreira, Tais Nobrega de Sousa, Gabriel W. Rangel, Igor C. Johansen, Rodrigo M. Corder, Simone Ladeia-Andrade, José Pedro Gil
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    Yonne F Chehuan, Monica RF Costa, Jacqueline S Costa, Maria GC Alecrim, Fátima Nogueira, Henrique Silveira, Larissa W Brasil, Gisely C Melo, Wuelton M Monteiro, Marcus VG Lacerda
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    Fernanda G Versiani, Maria EM Almeida, Gisely C Melo, Francivaldo OL Versiani, Patrícia P Orlandi, Luís André M Mariúba, Leidiane A Soares, Luciana P Souza, Antonio A da Silva Balieiro, Wuelton M Monteiro, Fabio TM Costa, Hernando A del Portillo, Marcus V
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Trichinella spiralis Infection Suppressed Gut Inflammation with CD4+CD25+Foxp3+ T Cell Recruitment
Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Seon Hee Choi, Soon Cheol Ahn, Hak Sun Yu
Korean J Parasitol 2012;50(4):385-390.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.385

In order to know the effect of pre-existing Trichinella spiralis infection on experimentally induced intestinal inflammation and immune responses, we induced colitis in T. spiralis-infected mice and observed the severity of colitis and the levels of Th1, Th2, and regulatory cytokines and recruitment of CD4+CD25+Foxp3+ T (regulatory T; Treg) cells. Female C57BL/6 mice were infected with 250 muscle larvae; after 4 weeks, induction of experimental colitis was performed using 3% dextran sulfate sodium (DSS). During the induction period, we observed severity of colitis, including weight loss and status of stool, and evaluated the disease activity index (DAI). A significantly low DAI and degree of weight loss were observed in infected mice, compared with uninfected mice. In addition, colon length in infected mice was not contracted, compared with uninfected mice. We also observed a significant increase in production of pro-inflammatory cytokines, IL-6 and IFN-γ, in spleen lymphocytes treated with DSS; however, such an increase was not observed in infected mice treated with DSS. Of particular interest, production of regulatory cytokines, IL-10 and transforming growth factor (TGF)-β, in spleen lymphocytes showed a significant increase in mice infected with T. spiralis. A similar result was observed in mesenteric lymph nodes (MLN). Subsets of the population of Treg cells in MLN and spleen showed significant increases in mice infected with T. spiralis. In conclusion, T. spiralis infection can inhibit the DSS-induced colitis in mice by enhancing the regulatory cytokine and Treg cells recruitment.

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Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
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Korean J Parasitol 2012;50(4):391-394.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.391

The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.

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