Parasites, Hosts and Diseases

"Aleksandro S. Da Silva"

Original Article

Oxidative Stress in the Heart of Rats Infected with Trypanosoma evansi: Matheus D. Baldissera, Carine de F. Souza, Cl?udia M. Bertoncheli, Karine L. da Silveira, Thirssa H. Grando, Bianca C. Z. Porto, Daniela B. R. Leal, Aleksandro S. Da Silva, Ricardo E. Mendes, Lenita M. Stefani, Silvia G. Monteiro; Korean J Parasitol 2016;54(3):247-252.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.247

Abstract
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This study was conducted to investigate the occurrence of oxidative stress in the heart tissue of rats infected with Trypanosoma evansi. Rats were divided into 2 groups (A and B) with 12 animals each, and further subdivided into 4 subgroups (A1 and A2, 6 animals/each; and B1 and B2, 6 animals/each). Animals in the groups B1 and B2 were subcutaneously inoculated with T. evansi. Thiobarbituric acid reactive substances (TBARS), superoxide dismutase activity (SOD), glutathione S-transferase activity (GST), reduced glutathione activity (GSH), and non-protein thiols (NPSH) in the heart tissue were evaluated. At day 5 and 15 post-infection (PI), an increase in the TBARS levels and a decrease in the SOD activity (P<0.05) were observed. GSH and GST activities were decreased in infected animals at day 15 PI (P<0.05). Considering the proper functioning of the heart, it is possible that the changes in the activity of these enzymes involved in the oxidative stress may be related, at least in part, in the pathophysiology of rats infected with T. evansi.

Citations

Citations to this article as recorded by

Novel insights into antioxidant status, gene expression, and immunohistochemistry in an animal model infected with camel-derived Trypanosoma evansi and Theileria annulata
Reem M. Ramadan, Alaa F. Bakr, Esraa Fouad, Faten F. Mohammed, Azza M. Abdel-Wahab, Sahar Z. Abdel-Maogood, Mohamed M. El-Bahy, Mai A. Salem
Parasites & Vectors.2024;[Epub] CrossRef
Molecular and phylogenetic analysis of a type K1 strain Trypanosoma evansi isolate from Nigerian cattle: An evaluation of the therapeutic effects of compounds from Brassica oleracea on the histopathology of infected wister rats
Kingsley Onyekachi Moh, Sodangi Abdulkarim Luka, Iliya Shehu Ndams, Idris Alao Lawal, Dahiru Sani, Sylvester Sunday Obeta, Gbenga Peter Oderinde, Emeka John Dingwoke, Fatima Amin Adamude, Abraham Ehinomhen Ubhenin, Saifullahi Umar
Biochemistry and Biophysics Reports.2023; 33: 101424. CrossRef
The disturbance of antioxidant/oxidant balance in fish experimentally infected by Aeromonas caviae: Relationship with disease pathophysiology
Matheus D. Baldissera, Carine F. Souza, Belisa Parmeggiani, Guilhian Leipnitz, Camila Marina Verdi, RobertoC.V. Santos, Lenita M. Stefani, Bernardo Baldisserotto
Microbial Pathogenesis.2018; 122: 53. CrossRef
Relationship between antioxidant defense in Acanthamoeba spp. infected lungs and host immunological status
N. Łanocha-Arendarczyk, I. Baranowska-Bosiacka, I. Gutowska, K. Kot, E. Metryka, D.I. Kosik-Bogacka
Experimental Parasitology.2018; 193: 58. CrossRef
Achyrocline satureioides essential oil loaded in nanocapsules ameliorate the antioxidant/oxidant status in heart of rats infected with Trypanosoma evansi
Carine F. Souza, Matheus D. Baldissera, Luciana F. Cossetin, Daiane F. Dalla Lana, Silvia G. Monteiro
Microbial Pathogenesis.2017; 105: 30. CrossRef

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Brief Communications

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii: Francine C. Paim, Aleksandro S. Da Silva, Carlos Breno V. Paim, Raqueli T. Fran?a, M?rcio M. Costa, Marta M. M. F. Duarte, Manuela B. Sangoi, Rafael N. Moresco, Silvia G. Monteiro, Sonia Terezinha A. Lopes; Korean J Parasitol 2013;51(1):133-137.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.133

Abstract
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This study aimed to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NO_x) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-γ, TNF-α, IL-1, IL-6, and NO_x. Cytokines were assessed by ELISA quantitative sandwich technique, and NO_x was measured by the modified Griess method. Cytokine levels (IFN-γ, TNF-α, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NO_x were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

Citations

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Andrew Leisewitz, Amelia Goddard, Jill De Gier, Jessica Van Engelshoven, Sarah Clift, Peter Thompson, Johan P. Schoeman
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Kristen R. Richter, Amirah N. Nasr, Angela M. Mexas
Veterinary Pathology.2018; 55(1): 53. CrossRef
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Martina Crnogaj, José Joaquin Cerón, Iva Šmit, Ivana Kiš, Jelena Gotić, Mirna Brkljačić, Vesna Matijatko, Camila Peres Rubio, Nada Kučer, Vladimir Mrljak
BMC Veterinary Research.2017;[Epub] CrossRef
Serum biochemistry profile, inflammatory cytokines, adipokines and cardiovascular findings in obese dogs
Diego Piantedosi, Antonio Di Loria, Jacopo Guccione, Angela De Rosa, Silvia Fabbri, Laura Cortese, Sergio Carta, Paolo Ciaramella
The Veterinary Journal.2016; 216: 72. CrossRef
Excessive Pro-Inflammatory Serum Cytokine Concentrations in Virulent Canine Babesiosis
Amelia Goddard, Andrew L. Leisewitz, Mads Kjelgaard-Hansen, Annemarie T. Kristensen, Johan P. Schoeman, Michelle L. Baker
PLOS ONE.2016; 11(3): e0150113. CrossRef
Canine rangeliosis due to Rangelia vitalii: From first report in Brazil in 1910 to current day – A review
Raqueli T. França, Aleksandro S. Da Silva, Alexandre P. Loretti, Cinthia M. Mazzanti, Sonia T.A. Lopes
Ticks and Tick-borne Diseases.2014; 5(5): 466. CrossRef
Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis
Wojciech Zygner, Olga Gójska-Zygner, Piotr Bąska, Ewa Długosz
Parasitology Research.2014; 113(4): 1499. CrossRef
Increase nitric oxide and oxidative stress in dogs experimentally infected by Ehrlichia canis: Effect on the pathogenesis of the disease
Aleksandro S. Da Silva, Thiago D. Munhoz, Joice L.M. Faria, Giovanni Vargas-Hérnandez, Rosangela Z. Machado, Taís C. Almeida, Rafael N. Moresco, Lenita M. Stefani, Mirela Tinucci-Costa
Veterinary Microbiology.2013; 164(3-4): 366. CrossRef

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Susceptibility of Mice to Trypanosoma evansi Treated with Human Plasma Containing Different Concentrations of Apolipoprotein L-1: Aleksandro S. Da Silva, Vinicius R. Fanfa, Mateus A. Otto, Lucas T. Gressler, Kaio C.S. Tavares, C?cera R. Lazzarotto, Alexandre A. Tonin, Luiz C. Miletti, Marta M.M.F. Duarte, Silvia G. Monteiro; Korean J Parasitol 2011;49(4):427-430.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.427

Abstract
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The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a strain of T. evansi and human plasma (plasmas 1, 2, and 3) from 3 adult males clinically healthy were used. In vivo test used 50 mice divided in 5 groups (A to E) with 10 animals in each group. Animals of groups B to E were infected, and then treated with 0.2 ml of human plasma in the following outline: negative control (A), positive control (B), treatment with plasma 1 (C), treatment with plasma 2 (D), and treatment with plasma 3 (E). Mice treated with human plasma showed an increase in longevity of 40.9±0.3 (C), 20±9.0 (D) and 35.6±9.3 (E) days compared to the control group (B) which was 4.3±0.5 days. The number of surviving mice and free of the parasite (blood smear and PCR negative) at the end of the experiment was 90%, 0%, and 60% for groups C, D, and E, respectively. The quantification of APOL1 was performed due to the large difference in the treatments that differed in the source plasma. In plasmas 1, 2, and 3 was detected the concentration of 194, 99, and 115 mg/dl of APOL1, respectively. However, we believe that this difference in the treatment efficiency is related to the level of APOL1 in plasmas.

Citations

Citations to this article as recorded by

The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo
Xuan Zhang, Xiao-Kun Hong, Su-Jin Li, De-Hua Lai, Geoff Hide, Zhao-Rong Lun, Yan-Zi Wen
Experimental Parasitology.2018; 184: 115. CrossRef
Pre-treatment with curcumin modulates acetylcholinesterase activity and proinflammatory cytokines in rats infected with Trypanosoma evansi
Patrícia Wolkmer, Cássia B. da Silva, Francine C. Paim, Marta M.M.F. Duarte, Verônica Castro, Heloisa E. Palma, Raqueli T. França, Diandra V. Felin, Lucas C. Siqueira, Sonia T.A. Lopes, Maria Rosa C. Schetinger, Silvia G. Monteiro, Cinthia M. Mazzanti
Parasitology International.2013; 62(2): 144. CrossRef