Parasites, Hosts and Diseases

"Dong-Il Chung"

Brief Communication

Glucose-6-phosphate dehydrogenase variants in Kachin, Myanmar: Zin Moon, Ja Moon Aung, Dorene VanBik, Hae Soo Yun, Sanghyun Lee, Sylvatrie-Danne Dinzouna-Boutamba, Zau Ring, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo; Parasites Hosts Dis 2025;63(4):360-363.
Published online November 19, 2025; DOI: https://doi.org/10.3347/PHD.25053

Abstract
PDF

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked recessive genetic disorder that can cause severe anemia in affected individuals exposed to oxidative stress. This risk is particularly relevant in patients treated with the antimalarial drug primaquine. In Myanmar, primaquine has been widely administered as a Plasmodium vivax malaria treatment; however, prevalence of G6PD deficiency among the population remains insufficiently characterized. This study investigated the prevalence of G6PD variants among various minority ethnic subgroups residing in Kachin State, Myanmar. Blood samples from 440 participants were analyzed; however, the Mahidol variant (G487A) was identified in 21 individuals (4.8%). A major limitation of this study was the absence of G6PD enzyme activity data to confirm whether the Mahidol variant induces G6PD deficiency.

275 View
11 Download

Original Articles

Prevalence and molecular analysis of glucose-6-phosphate dehydrogenase deficiency in Chin State, Myanmar: Ja Moon Aung, Zin Moon, Dorene VanBik, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zau Ring, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo; Parasites Hosts Dis 2023;61(2):154-162.
Published online May 23, 2023; DOI: https://doi.org/10.3347/PHD.23004

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is caused by X-linked recessive disorderliness. It induces severe anemia when a patient with G6PD deficiency is exposed to oxidative stress that occurs with administration of an antimalarial drug, primaquine. The distribution of G6PD deficiency remains unknown while primaquine has been used for malaria treatment in Myanmar. This study aimed to investigate the prevalence of G6PD deficiency and its variants in Chin State, Myanmar. Among 322 participants, 18 (11 males and 7 females) demonstrated a G6PD deficiency. Orissa variant was dominant in the molecular analysis. This would be related to neighboring Indian and Bangladeshi population, in which Orissa variant was also reported as the main mutation type. The screening test for G6PD deficiency before primaquine treatment appears to be important in Myanmar.

Citations

Citations to this article as recorded by

Glucose-6-phosphate dehydrogenase variants in Kachin, Myanmar
Zin Moon, Ja Moon Aung, Dorene VanBik, Hae Soo Yun, Sanghyun Lee, Sylvatrie-Danne Dinzouna-Boutamba, Zau Ring, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo
Parasites, Hosts and Diseases.2025; 63(4): 360. CrossRef
Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates: Correspondence
Amnuay Kleebayoon, Viroj Wiwanitkit
Parasites, Hosts and Diseases.2023; 61(3): 338. CrossRef

4,307 View
176 Download
2 Web of Science
Crossref

Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates from central Myanmar: Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zin Moon, Dong-Il Chung, Yeonchul Hong, Moe Kyaw Myint, Haung Naw, Byoung-Kuk Na, Youn-Kyoung Goo; Parasites Hosts Dis 2023;61(1):24-32.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22165

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the polymorphic var multigene family, is a highly polymorphic antigen that plays a crucial role in the pathology of malaria. The contribution of the genetic diversity of var toward the immune escape of P. falciparum has not yet been fully elucidated. This study aimed to characterize the diversity of var repertoires by screening P. falciparum Duffy-binding-like α domain (PfDBLα) among field isolates from central Myanmar. Genetic analysis revealed that the D-H segments of var in Myanmar populations have an extensive polymorphic repertoire, with high numbers of unique sequence types in each individual. However, var genes from the global population, including Myanmar, shared close genetic lineages regardless of their geographic origins, indicating that they have not undergone rapid evolutionary changes.

Citations

Citations to this article as recorded by

Exploring the Potential of miRNA-92a-3p as Lead for Sequence-Based Therapies for Malaria
Sowmya R. Prabhu, Sayandrila Paul, Shashikiran Umakanth, Manjunath Hande, Abdul Vahab Saadi, Himanshu Gupta, Kapaettu Satyamoorthy
Acta Parasitologica.2025;[Epub] CrossRef
Genetic diversity of Plasmodium falciparum erythrocyte membrane protein 1 in field isolates: Correspondence
Amnuay Kleebayoon, Viroj Wiwanitkit
Parasites, Hosts and Diseases.2023; 61(3): 338. CrossRef

5,300 View
183 Download
3 Web of Science
Crossref

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein: So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2022;60(1):1-6.
Published online February 23, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.1.1

Abstract
PDF

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein—Acanthamoeba silent-information regulator 2-like protein (AcSir2)—was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 μM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 μM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

Citations

Citations to this article as recorded by

Pterostilbene: A natural neuroprotective stilbene with anti-Alzheimer's disease properties
Songlan Gao, Honglei Zhang, Na Li, Lijuan Zhang, Zhe Zhu, Changlu Xu
Journal of Pharmaceutical Analysis.2025; 15(4): 101043. CrossRef
Alzheimer’s Disease: A Review of Molecular Mechanisms and Therapeutic Implications by Targeting Sirtuins, Caspases, and GSK-3
Kalpana Pandya, Krishnashish Roul, Avanish Tripathi, Sateesh Belemkar, Anshuman Sinha, Meryem Erol, Devendra Kumar
ACS Chemical Neuroscience.2025; 16(12): 2178. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells
Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha
Acta Tropica.2024; 257: 107288. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef

4,716 View
252 Download
5 Web of Science
Crossref

Case Reports

A Retrieved Sparganum of Spirometra erinaceieuropaei from a Korean Man during Mechanical Thrombectomy: Yang-Ha Hwang, Wonsoo Son, Yong-Won Kim, Dong-Hun Kang, Hyun-Ha Chang, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung; Korean J Parasitol 2020;58(3):309-313.
Published online June 26, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.3.309

Abstract
PDF

Human sparganosis is a zoonotic disease caused by infection and migration of the plerocercoid of Spirometra spp. Although sparganosis were reported from most parts of the body, the sparganum parasitizing inside cerebral artery is remarkably uncommon. We report a case of cerebral intravascular sparganosis in an elderly patient with acute ischemic stroke who was diagnosed by retrieving sparganum during mechanical thrombectomy. Finally, the parasites were identified as Spirometra erinaceieuropaei using multiplex PCR and cox1 gene sequencing.

Citations

Citations to this article as recorded by

Neglected zoonotic helminthiases in wild canids: new insights from South America
Manuel Uribe, Jan Brabec, Jenny J. Chaparro-Gutiérrez, Carlos Hermosilla
Frontiers in Veterinary Science.2023;[Epub] CrossRef

5,921 View
105 Download
1 Web of Science
Crossref

Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea: Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2019;57(4):417-422.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.417

Abstract
PDF

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

Citations

Citations to this article as recorded by

Exploring bioactive molecules released during inter- and intraspecific competition: A paradigm for novel antiparasitic drug discovery and design for human use
Pichet Ruenchit
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100256. CrossRef
Taeniasis impacts human gut microbiome composition and function
Wenjie Mu, Pingping Ma, Yugui Wang, Yaqi Li, Yingying Ding, Yang Zou, Lixia Pu, Qi Yan, Haoyue Kong, Xiaola Guo, Aijiang Guo, Hailong Li, Shuai Wang
The ISME Journal.2024;[Epub] CrossRef
Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China
Xiu-Min Han, Xue-Yong Zhang, Ying-Na Jian, Qing-Shan Tian
The Korean Journal of Parasitology.2021; 59(3): 311. CrossRef

11,888 View
239 Download
4 Web of Science
Crossref

Original Article

Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii: Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, H??ng Giang L?, Th? Lam Th?i, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2018;56(5):409-418.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.409

Abstract
PDF

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet. In this study, we isolated a gene encoding cysteine protease of A. castellanii (AcCP) and its biochemical and functional properties were analyzed. Sequence analysis of AcCP suggests that this enzyme is a typical cathepsin L family cysteine protease, which shares similar structural characteristics with other cathepsin L-like enzymes. The recombinant AcCP showed enzymatic activity in acidic conditions with an optimum at pH 4.0. The recombinant enzyme effectively hydrolyzed human proteins including hemoglobin, albumin, immunoglobuins A and G, and fibronectin at acidic pH. AcCP mainly localized in lysosomal compartment and its expression was observed in both trophozoites and cysts. AcCP was also identified in cultured medium of A. castellanii. Considering to lysosomal localization, secretion or release by trophozoites and continuous expression in trophozoites and cysts, the enzyme could be a multifunctional enzyme that plays important biological functions for nutrition, development and pathogenicity of A. castellanii. These results also imply that AcCP can be a promising target for development of chemotherapeutic drug for Acanthamoeba infections.

Citations

Citations to this article as recorded by

Acanthamoeba castellanii cysteine protease 3 promotes M1 macrophage polarization through the TLR4/NF‑κB pathway
Zhi-xin Wang, Wan-jun Jiao, Mian-jing Wang, Yong Yang, Hai-long Wang, Hong-li Liu
Parasites & Vectors.2025;[Epub] CrossRef
Unraveling the interplay between unicellular parasites and bacterial biofilms: Implications for disease persistence and antibiotic resistance
Eva Zanditenas, Serge Ankri
Virulence.2024;[Epub] CrossRef
Epidemiology of and Genetic Factors Associated with Acanthamoeba Keratitis
Muhammad Ilyas, Fiona Stapleton, Mark D. P. Willcox, Fiona Henriquez, Hari Kumar Peguda, Binod Rayamajhee, Tasbiha Zahid, Constantinos Petsoglou, Nicole A. Carnt
Pathogens.2024; 13(2): 142. CrossRef
Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model
Rubén L. Rodríguez-Expósito, Ines Sifaoui, Lizbeth Salazar-Villatoro, Carlos J. Bethencourt-Estrella, José J. Fernández, Ana R. Díaz-Marrero, Robert Sutak, Maritza Omaña-Molina, José E. Piñero, Jacob Lorenzo-Morales
Marine Drugs.2024; 22(9): 423. CrossRef
The gene expression and proteomic profiling of Acanthamoeba isolates
Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta
Experimental Parasitology.2023; 255: 108630. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Induction of Programmed Cell Death in Acanthamoeba culbertsoni by the Repurposed Compound Nitroxoline
Rubén L. Rodríguez-Expósito, Ines Sifaoui, María Reyes-Batlle, Frieder Fuchs, Patrick L. Scheid, José E. Piñero, Robert Sutak, Jacob Lorenzo-Morales
Antioxidants.2023; 12(12): 2081. CrossRef
Paradigms of Protist/Bacteria Symbioses Affecting Human Health: Acanthamoeba species and Trichomonas vaginalis
Fiona L. Henriquez, Ronnie Mooney, Timothy Bandel, Elisa Giammarini, Mohammed Zeroual, Pier Luigi Fiori, Valentina Margarita, Paola Rappelli, Daniele Dessì
Frontiers in Microbiology.2021;[Epub] CrossRef
Trogocytosis in Unicellular Eukaryotes
Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
Cells.2021; 10(11): 2975. CrossRef
Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics
Issam Hasni, Philippe Decloquement, Sandrine Demanèche, Rayane Mouh Mameri, Olivier Abbe, Philippe Colson, Bernard La Scola
Microorganisms.2020; 8(5): 771. CrossRef
Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng
Parasites & Vectors.2020;[Epub] CrossRef
Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins
Abigail Betanzos, Cecilia Bañuelos, Esther Orozco
Genes.2019; 10(8): 618. CrossRef

8,359 View
168 Download
13 Web of Science
Crossref

Case Report

A Case of Furuncular Myiasis Due to Cordylobia anthropophaga in a Korean Traveler Returning from Uganda: Su-Min Song, Shin-Woo Kim, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2017;55(3):327-331.
Published online June 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.3.327

Abstract
PDF

A fly larva was recovered from a boil-like lesion on the left leg of a 33-year-old male on 21 November 2016. He has worked in an endemic area of myiasis, Uganda, for 8 months and returned to Korea on 11 November 2016. The larva was identified as Cordylobia anthropophaga by morphological features, including the body shape, size, anterior end, posterior spiracles, and pattern of spines on the body. Subsequent 28S rRNA gene sequencing showed 99.9% similarity (916/917 bp) with the partial 28S rRNA gene of C. anthropophaga. This is the first imported case of furuncular myiasis caused by C. anthropophaga in a Korean overseas traveler.

Citations

Citations to this article as recorded by

Imported parasitic diseases in the Republic of Korea: status and issues
Jong-Yil Chai
Journal of the Korean Medical Association.2025; 68(1): 52. CrossRef
Origins and Diversification of Myiasis Across Blowflies
Gisele Antoniazzi Cardoso, Vanessa A. S. Cunha, Bruno C. Genevcius, Tais Madeira‐Ott, Bárbara Maria de Andrade Costa, Daniela Munhoz Rossoni, Patricia Jacqueline Thyssen, Tatiana Teixeira Torres
Ecology and Evolution.2025;[Epub] CrossRef
Incidence of Furuncular Myiasis due to Cordylobia anthropophaga (Blanchard, 1872) Larvae Infestation and Associated Risk Factors on Dogs in Ilorin Metropolis, North Central, Nigeria
Musa Rabiu, Ridwan Usman Adekola, Shola David Ola-Fadunsin, Isau Aremu Ganiyu, Miftah Olalekan Adeleke, Idiat Modupe Sanda, Ghali-Mohammed Ibraheem, Isyaku Abdulmajeed
UMYU Scientifica.2025; 4(1): 408. CrossRef
Human myiasis in Sub-Saharan Africa: A systematic review
Binta J. J. Jallow, Goudja Gassara, Ousman Bajinka, Yifei Luo, Mandie Liu, Jifeng Cai, Jingjing Huang, Fanming Meng, Nigel Beebe
PLOS Neglected Tropical Diseases.2024; 18(3): e0012027. CrossRef
Scrotal Myiasis in a Child Due to Cordylobia anthropophaga
Majed H Wakid, Yasser S Sharafeldein, Angham A Almakki, Dhuha A Alidrisi, Abeer A Bashinim
Cureus.2024;[Epub] CrossRef
Use of Commercial Bacon to Extract Maggots from an Infested Chronic Wound
Gregory Fenati, Santana Youssoffi, Shriya Vejendla, Cameron C. Neeki, Vinh T. Nguyen, Fanglong Dong, Michael M. Neeki
Advances in Skin & Wound Care.2024; 37(10): 555. CrossRef
Cutaneous myiasis by Calliphoridae dipterans in dogs from Chad
Rafael A.N. Ramos, Hassan Hakimi, Sidouin K. Metinou, Wachellet Danzabe, Macon Overcast, Jeremiah Cox, Rebecca Garabed, Philip Tchindebet Ouakou, Richard Ngandolo Bongo Nare, Fernando Torres-Velez, Lucienne Tritten, Meriam N. Saleh, Guilherme G. Verocai
Acta Tropica.2024; 260: 107454. CrossRef
Cordylobia anthropophaga Myiasis Mimicking Hyperproliferative Skin Disorder in Traveler Returning from Sub-Saharan Africa
Lidija Popović Dragonjić, Andrija Jović, Irena Janković, Jelena Miladinović, Aleksandar Ranković, Maja Cvetanović, Relja Beck, Dinko Novosel, Thomas Pape, Pavle Banović
Tropical Medicine and Infectious Disease.2023; 8(11): 505. CrossRef
Elephantoloemus indicus Austen, 1930 (Diptera: Calliphoridae) as the cause of cutaneous myiasis in captive Indian elephants from Assam, India
Prabhat Chandra Sarmah, Saidul Islam, Dilip Kumar Deka, Kanta Bhattacharjee, Kuntola Roy
Veterinary Parasitology: Regional Studies and Reports.2022; 32: 100734. CrossRef
Clogmia albipunctata (Nematocera; Psychodidae) as the Etiologic Agent of Myiasis: True or False?
Mohammad Akhoundi, Nambininiavo Marianne Ranorohasimanana, Sophie Brun, Catherine Kauffmann-Lacroix, Arezki Izri
Diagnostics.2022; 12(9): 2129. CrossRef
CRANIAL OSTEOMYELITIS AS A COMPLICATION OF FURUNCULAR MYIASIS
Nelson Muñoz, Sandra Galvis, Oscar Patiño, Carlos Moneriz
Revista Paulista de Pediatria.2021;[Epub] CrossRef
Human myiasis cases originating and reported in africa for the last two decades (1998–2018): A review
Simon K. Kuria, Adebola O. Oyedeji
Acta Tropica.2020; 210: 105590. CrossRef
Furuncular myiasis for the Western dermatologist: treatment in outpatient consultation
Romain Blaizot, Christophe Vanhecke, Philippe Le Gall, Alexandre Duvignaud, Marie‐Catherine Receveur, Denis Malvy
International Journal of Dermatology.2018; 57(2): 227. CrossRef
First case of Furuncular Myiasis due to Cordylobia anthropophaga in a Latin American resident returning from Central African Republic
Jóse A. Suárez, Argentina Ying, Luis A. Orillac, Israel Cedeño, Néstor Sosa
The Brazilian Journal of Infectious Diseases.2018; 22(1): 70. CrossRef
A Case of Cutaneous Myiasis Caused by Cordylobia anthropophaga Larvae in a Korean Traveler Returning from Central Africa
Joo Yeon Ko, In-Yong Lee, Byeong Jin Park, Jae Min Shin, Jae-Sook Ryu
The Korean Journal of Parasitology.2018; 56(2): 199. CrossRef

13,966 View
161 Download
17 Web of Science
Crossref

Original Article

Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation: Ui-han Son, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Hae Soo Yun, Jung-Yeon Kim, So-Young Joo, Sookwan Jeong, Man Hee Rhee, Yeonchul Hong, Dong-Il Chung, Dongmi Kwak, Youn-Kyoung Goo; Korean J Parasitol 2017;55(2):149-158.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.149

Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (π and Θ_w), and Tajima’s D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima’s D values for vir 27 (1.08530, p>0.1), vir 12 (2.89007, p<0.01), and vir 21 (0.40782, p>0.1) were positive, and that of vir 4 (-1.32162, p>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.

Citations

Citations to this article as recorded by

Genetic diversity and natural selection analysis of VAR2CSA and vir genes: implication for vaccine development
Joseph Hawadak, Aditi Arya, Shewta Chaudhry, Vineeta Singh
Genomics & Informatics.2024;[Epub] CrossRef
Population genetic analysis of Plasmodium vivax vir genes in Pakistan
Sylvatrie-Danne Dinzouna-Boutamba, Zin Moon, Sanghyun Lee, Sahib Gul Afridi, Hương Giang Lê, Yeonchul Hong, Byoung-Kuk Na, Youn-Kyoung Goo
Parasites, Hosts and Diseases.2024; 62(3): 313. CrossRef
Immunological characterization of a VIR protein family member (VIR-14) in Plasmodium vivax-infected subjects from different epidemiological regions in Africa and South America
Raianna F. Fantin, Camila H. Coelho, Anne D. Berhe, Luisa M. D. Magalhães, Dhélio B. Pereira, Nichole D. Salinas, Niraj H. Tolia, Chanaki Amaratunga, Seila Suon, Issaka Sagara, David L. Narum, Ricardo T. Fujiwara, Claudia Abejon, Antonio Campos-Neto, Patr
PLOS Neglected Tropical Diseases.2023; 17(4): e0011229. CrossRef
Vivax Malaria and the Potential Role of the Subtelomeric Multigene vir Superfamily
Youn-Kyoung Goo
Microorganisms.2022; 10(6): 1083. CrossRef
Genetic polymorphism of vir genes of Plasmodium vivax in Myanmar
Byoung-Kuk Na, Tong-Soo Kim, Khin Lin, Moon-Chang Baek, Dong-Il Chung, Yeonchul Hong, Youn-Kyoung Goo
Parasitology International.2021; 80: 102233. CrossRef
Humoral and cellular immune response to Plasmodium vivax VIR recombinant and synthetic antigens in individuals naturally exposed to P. vivax in the Republic of Korea
Sanghyun Lee, Young-Ki Choi, Youn-Kyoung Goo
Malaria Journal.2021;[Epub] CrossRef
Succinate dehydrogenase gene as a marker for studying Blastocystis genetic diversity
Adriana Higuera, Marina Muñoz, Myriam Consuelo López, Patricia Reyes, Plutarco Urbano, Oswaldo Villalobos, Juan David Ramírez
Heliyon.2020; 6(11): e05387. CrossRef
A bite to fight: front-line innate immune defenses against malaria parasites
Stephanie Tannous, Esther Ghanem
Pathogens and Global Health.2018; 112(1): 1. CrossRef
Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea
Young Yil Bahk, Jeonga Kim, Seong Kyu Ahn, Byoung-Kuk Na, Jong-Yil Chai, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(6): 545. CrossRef

11,996 View
155 Download
9 Web of Science
Crossref

Brief Communication

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(3):329-334.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.329

Abstract
PDF

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

Citations

Citations to this article as recorded by

Loop‐Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique I. Andersson
Microbial Biotechnology.2025;[Epub] CrossRef
A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis
Zhenke Yang, Jinghui Wang, Yiming Qi, Yiping Shi, Fakun Li, Weijuan Wang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang, Shuai Wang
Parasites & Vectors.2024;[Epub] CrossRef
A novel fluoro colorimetric Loop mediated isothermal amplification (LAMP) assay for detection of Trichomonas vaginalis
Shoorashetty Manohar Rudresh, Pareyam Pooja, Pattacheravanda Nanaiah Shakuntala, Kanta Madhu
Indian Journal of Medical Microbiology.2024; 49: 100610. CrossRef
Establishment of a programmatic detection method for Trichomonas vaginalis based on double antibody sandwich ELISA targeting TvCP39 antigen
Yuhua Li, Fakun Li, Wenjie Tian, Yani Zhang, Weijuan Wang, Zhenke Yang, Xiaowei Tian, Shuai Wang, Xuefang Mei, Zhenchao Zhang
Acta Tropica.2024; 260: 107489. CrossRef
Label-free electrochemical DNA biosensing of MR TV 29 18s ribosomal RNA gene of Trichomonas vaginalis by signalization of non-spherical gold nanoparticles
R. Dehdari Vais, H. Heli, N. Sattarahmady
Materials Today Communications.2023; 34: 105123. CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene
Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang
Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
A novel and ultrasensitive label-free electrochemical DNA biosensor for Trichomonas vaginalis detection based on a nanostructured film of poly(ortho-aminophenol)
Rezvan Dehdari Vais, Hossein Heli, Naghmeh Sattarahmady, Afshin Barazesh
Synthetic Metals.2022; 287: 117082. CrossRef
Omics Analyses of Trichomonas vaginalis Actin and Tubulin and Their Participation in Intercellular Interactions and Cytokinesis
Sebastián Lorenzo-Benito, Luis Alberto Rivera-Rivas, Lizbeth Sánchez-Ayala, Jaime Ortega-López, Octavio Montes-Flores, Daniel Talamás-Lara, Rossana Arroyo
Genes.2022; 13(6): 1067. CrossRef
Photo-genosensor for Trichomonas vaginalis based on gold nanoparticles-genomic DNA
S. Ilbeigi, R. Dehdari Vais, N. Sattarahmady
Photodiagnosis and Photodynamic Therapy.2021; 34: 102290. CrossRef
Loop mediated isothermal amplification assay for detection of Trichomonas vaginalis in vaginal swabs among symptomatic women from North India
S. Khurana, R. Dadwal, N. Sharma, A. Mewara, S. Singh, R. Bagga, R. Yadav, S. Sethi
Letters in Applied Microbiology.2020; 70(3): 196. CrossRef
Establishment and application of isothermal amplification techniques for the detection of heat-stable I enterotoxin of enterotoxigenic Escherichia coli
Junjun Zhai, Zhang Yan, Feng Ping, Qu Lei, Xuelong Chen, Yanping Qi, Tianwen Wang
PLOS ONE.2020; 15(4): e0230881. CrossRef
Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65
Yuhua Li, Shuai Wang, Haoran Li, Xiaoxiao Song, Hao Zhang, Yujuan Duan, Chengyang Luo, Bingli Wang, Sifan Ji, Qing Xie, Zhenchao Zhang
BMC Infectious Diseases.2020;[Epub] CrossRef
Label-free ultrasensitive electrochemical genosensing of Trichomonas vaginalis using anisotropic-shaped gold nanoparticles as a platform, a repeated sequence of the parasite DNA as a probe, and toluidine blue as a redox marker
N. Delshadi-Jahromi, R. Nazari-Vanani, H. Yadegari, N. Sattarahmady, G.R. Hatam, H. Heli
Sensors and Actuators B: Chemical.2018; 273: 234. CrossRef
Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei
Shao-Xin Cai, Fan-De Kong, Shu-Fei Xu, Cui-Luan Yao
PeerJ.2018; 6: e5993. CrossRef

10,601 View
196 Download
15 Web of Science
Crossref

Original Article

Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2016;54(2):133-138.
Published online April 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.2.133

Abstract
PDF

Encystation is an essential process for Acanthamoeba survival under nutrient-limiting conditions and exposure to drugs. The expression of several genes has been observed to increase or decrease during encystation. Epigenetic processes involved in regulation of gene expression have been shown to play a role in several pathogenic parasites. In the present study, we identified the protein arginine methyltransferase 5 (PRMT5), a known epigenetic regulator, in Acanthamoeba castellanii. PRMT5 of A. castellanii (AcPRMT5) contained domains found in S-adenosylmethionine-dependent methyltransferases and in PRMT5 arginine-N-methyltransferase. Expression levels of AcPRMT5 were increased during encystation of A. castellanii. The EGFP-PRMT5 fusion protein was mainly localized in the nucleus of trophozoites. A. castellanii transfected with siRNA designed against AcPRMT5 failed to form mature cysts. The findings of this study lead to a better understanding of epigenetic mechanisms behind the regulation of encystation in cyst-forming pathogenic protozoa.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease
Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui
Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Free-living amoebae and squatters in the wild: ecological and molecular features
Ascel Samba-Louaka, Vincent Delafont, Marie-Hélène Rodier, Estelle Cateau, Yann Héchard
FEMS Microbiology Reviews.2019; 43(4): 415. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii
Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2017; 55(2): 109. CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef

10,214 View
103 Download
10 Web of Science
Crossref

Brief Communication

Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea: Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2016;54(1):75-80.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.75

Abstract
PDF

This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR detected T. vaginalis in 12 (1.9%) patients. Testing via PCR demonstrated high sensitivity and high negative predictive value for T. vaginalis. Among the 19 women who tested positive for T. vaginalis according to PCR, 94.7% (18/19) reported vaginal signs and symptoms. Notably, more than 50% of T. vaginalis infections occurred in females younger than 30 years old, and 58% were unmarried. Multiplex PCR, which simultaneously detects pathogens from various sexually transmitted infections, revealed that 91.7% (11/12) of patients were infected with 2 or more pathogens. Mycoplasma hominis was the most prevalent co-infection pathogen with T. vaginalis, followed by Ureaplasma urealyticum and Chlamydia trachomatis. Our results indicate that PCR and multiplex PCR are the most sensitive tools for T. vaginalis diagnosis, rather than microscopy which has been routinely used to detect T. vaginalis infections in South Korea. Therefore, clinicians should take note of the high prevalence of T. vaginalis infections among adolescent and young women in order to prevent persistent infection and transmission of this disease.

Citations

Citations to this article as recorded by

Diagnostic accuracy of real-time polymerase chain reaction assay for the detection of Trichomonas vaginalis in clinical samples: A systematic review and meta-analysis
Emmanuel O. Babafemi, Benny P. Cherian, Khalid Rahman, Gilbert M. Mogoko, Oluwatoyin O. Abiola
African Journal of Laboratory Medicine.2025;[Epub] CrossRef
The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis
Zeinab Moghadamizad, Javad Yazdizadeh Khalili, Meysam Olfatifar, Milad Badri, Sasan Khazaei
International Health.2024; 16(3): 240. CrossRef
Combination of genitourinary candidiasis and sexually transmitted infections
N.E. Portnyagina, A.K. Kvardakova, V.V. Pakhomova, E.G. Gubanova, N.V. Deeva, I.G. Sergeeva
Russian Journal of Clinical Dermatology and Venereology.2024; 23(4): 377. CrossRef
Trichomonas vaginalis: comparison of primers for implementation as an in-house PCR in rural Vellore, South India
Nagarajan L. Surya, Thangamani Suji, Santhosh Rani, Irene Dorathy, Shantidani Minz, Rani Diana Sahni
BMC Infectious Diseases.2024;[Epub] CrossRef
Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran
Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari
BMC Women's Health.2023;[Epub] CrossRef
Comparison of Diagnostic Methods for Detection of Trichomonas vaginalis in Prediagnosed Vaginitis Cases and Its Association with Various Pathogens
Vildan Turan Faraşat, İbrahim Cüneyt Balcıoğlu, Pınar Solmaz Hasdemir, Ertaç Gümüş
Turkish Journal of Parasitology.2022; 46(3): 167. CrossRef
Trichomonas vaginalis follow-up and persistence in Colombian women
Lauren Hernández-Buelvas, Milena Camargo, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
Scientific Reports.2021;[Epub] CrossRef
Trichomoniasis in a tertiary hospital of Madrid, Spain (2013–2017): prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis
Celia Bolumburu, Vega Zamora, María Muñoz-Algarra, Francisca Portero-Azorín, José Antonio Escario, Alexandra Ibáñez-Escribano
Parasitology Research.2020; 119(6): 1915. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
PREVALENCE OF TRICHOMONIASIS IN ASYMPTOMATIC PREGNANT WOMEN POPULATION IN BANDUNG, WEST JAVA, INDONESIA
Pati Aji Achdiat, Reiva Farah Dwiyana, Vina Feriza, Rasmia Rowawi, Rendy Ariezal Effendi, Oki Suwarsa, Hendra Gunawan
Indonesian Journal of Tropical and Infectious Disease.2019; 7(4): 57. CrossRef
Prevalence of Trichomoniasis by PCR in Women Attending Health Screening in Korea
Seung-Ryong Kim, Jung-Hyun Kim, Na-Yeong Gu, Yong-Suk Kim, Yeon-Chul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2016; 54(2): 187. CrossRef
Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Hyun-Hee Kong, Won-Ki Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(3): 329. CrossRef
Epidemiology of Trichomoniasis in South Korea and Increasing Trend in Incidence, Health Insurance Review and Assessment 2009-2014
So-Young Joo, Youn-Kyoung Goo, Jae-Sook Ryu, Sang-Eun Lee, Won Kee Lee, Dong-Il Chung, Yeonchul Hong, Zhefeng Meng
PLOS ONE.2016; 11(12): e0167938. CrossRef

11,067 View
150 Download
11 Web of Science
Crossref

Case Reports

Comorbid Gastric Adenocarcinoma and Gastric and Duodenal Strongyloides stercoralis Infection: A Case Report: An Na Seo, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong, Ohkyoung Kwon, Han-Ik Bae; Korean J Parasitol 2015;53(1):95-99.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.95

Abstract
PDF

Strongyloides stercoralis can cause systemic infection, termed strongyloidiasis, and gastrointestinal ulcer disease in immunocompromised patients. However, to our knowledge, there are no reported cases of comorbid gastric adenocarcinoma and S. stercoralis infection. Here, we report a case of an 81-year-old Korean man who presented with S. stercoralis infection coexisting with early gastric adenocarcinoma (T1aN0M0). S. stercoralis eggs, rhabditiform larvae, and adult females were observed in normal gastric and duodenal crypts. They were also observed in atypical glands representative of adenocarcinoma and adenoma. Preliminary laboratory tests revealed mild neutrophilic and eosinophilic leukocytosis. A routine stool test failed to detect rhabditiform larvae in the patient’s fecal sample; however, S. stercoralis was identified by PCR amplification and 18S rRNA sequencing using genomic DNA extracted from formalin-fixed paraffin-embedded tissues. Postoperatively, the patient had a persistent fever and was treated with albendazole for 7 days, which alleviated the fever. The patient was followed-up by monitoring and laboratory testing for 4 months postoperatively, and no abnormalities were observed thus far. The fact that S. stercoralis infection may be fatal in immunocompromised patients should be kept in mind when assessing high-risk patients.

Citations

Citations to this article as recorded by

Helminth-induced immune modulation in colorectal cancer: exploring therapeutic applications
Hongyu Li, Chaojun Shan, Yunhuan Zhu, Xiaodong Yao, Lijun Lin, Xiaofen Zhang, Yuncheng Qian, Yuqing Wang, Jialu Xu, Yijie Zhang, Hairun Li, Ling Zhao, Keda Chen
Frontiers in Immunology.2025;[Epub] CrossRef
Relationship between parasites and lung cancer: Unveiling the link
Mariam T. El Khadrawe, Nahla El Skhawy, Maha M. Eissa
Tropical Medicine & International Health.2025; 30(7): 613. CrossRef
NEOPLASIA AND PROLIFERATIVE LESIONS IN FREE-RANGING MOUNTAIN AND GRAUER'S GORILLAS (GORILLA BERINGEI)
Maya L. Iyer, Kirsten V. Gilardi, Michael R. Cranfield, Sarah M. Corner, Eddy Kambale Syaluha, Jean Bosco Noheri, Benard Ssebide, Jean Felix Kinani, Fred Nizeyimana, Ricky Okwir Okello, Julius Nziza, Martin Kabuyaya, Methode Bahizi, Richard Muvunyi, Elisa
Journal of Zoo and Wildlife Medicine.2025;[Epub] CrossRef
Gastroduodenal Strongyloidiasis Diagnosed during Iron Deficiency Anemia Workup
Malek Kreidieh, Neville Mobarakai, Sherif Andrawes, Robert Colef, Lara Kreidieh, Hassan Al Moussawi, Jean M. Chalhoub
Case Reports in Gastroenterology.2025; 19(1): 467. CrossRef
The Parasitism and Tumors Carcinogenesis: A Review Subject
Ahmed Salih Alshewered
Acta Parasitologica.2024; 69(1): 183. CrossRef
A case of disseminated strongyloidiasis diagnosed by worms in the urinary sediment
Young-Ha Lee
Parasites, Hosts and Diseases.2024; 62(2): 238. CrossRef
Mining parasites for their potential as novel therapeutic agents against cancer
Neha Sylvia Walter, Shalmoli Bhattacharyya
Medical Oncology.2024;[Epub] CrossRef
Association between Some Helminths and Tumorigenesis through Immunological and Biochemical Factors
Maryam Fasihi-Karami, Reza Afrisham, Saina Karami, Ehsan Beigzadeh, Forough Kazemi
Current Cancer Therapy Reviews.2023; 19(2): 96. CrossRef
STRONGYLOIDES STERCORALIS: A RARE CASE DIAGNOSED WITH ESOPHAGEAL SWAB SAMPLE
Arzu Hazal AYDIN, Buse BAYAZIT GÖZÜKÜÇÜK, Nesrin TURHAN
Kırıkkale Üniversitesi Tıp Fakültesi Dergisi.2023; 25(1): 166. CrossRef
Is Gastric Involvement by Strongyloides stercoralis in an Immunocompetent Patient a Common Finding? A Case Report and Review of the Literature
Irene Pecorella, Tom Richard Okello, Gaia Ciardi, David Martin Ogwang
Acta Parasitologica.2022; 67(1): 94. CrossRef
Seropositivity Rates of Strongyloides stercoralis Antibody in the Southeastern Region of Republic of Korea: A Single-Center Retrospective Study
Taehwa Kim, Seungjin Lim
The Korean Journal of Parasitology.2022; 60(3): 181. CrossRef
Strongyloides Hyperinfection Causing Gastrointestinal Bleeding and Bacteremia in an Immunocompromised Patient
Juan Carlos De la Cruz Mayhua, Bisharah Rizvi
Cureus.2021;[Epub] CrossRef
Bacterial and Parasitic Pathogens as Risk Factors for Cancers in the Gastrointestinal Tract: A Review of Current Epidemiological Knowledge
Janneke W. Duijster, Eelco Franz, Jacques Neefjes, Lapo Mughini-Gras
Frontiers in Microbiology.2021;[Epub] CrossRef
Colorectal Cancer Related to Chronic Strongyloides stercoralis Infection
M. Sava, T. Huynh, A. Frugoli, L. Kong, M. Salehpour, B. Barrows
Case Reports in Gastrointestinal Medicine.2020; 2020: 1. CrossRef
Phylogenetic Positioning of a Strongyloides stercoralis Isolate Recovered from a Korean Patient and Comparison with Other Asian Isolates
Jaeho Bae, Mi Jin Jeong, Dong hoon Shin, Hyun Woo Kim, Sung Ho Ahn, Jun Ho Choi, Hak Sun Yu
The Korean Journal of Parasitology.2020; 58(6): 689. CrossRef
Microbiome signatures in prostate cancer
Sagarika Banerjee, James C Alwine, Zhi Wei, Tian Tian, Natalie Shih, Colin Sperling, Thomas Guzzo, Michael D Feldman, Erle S Robertson
Carcinogenesis.2019; 40(6): 749. CrossRef
Pathologic Features of Infectious Gastritis
Eric U. Yee, Enoch Kuo, Jeffrey D. Goldsmith
Advances in Anatomic Pathology.2018; 25(4): 238. CrossRef
The Clean India Mission: Public and animal health benefits
Rashmi Thakur, Balbir Bagicha Singh, Prateek Jindal, Rabinder Singh Aulakh, Jatinder Paul Singh Gill
Acta Tropica.2018; 186: 5. CrossRef
Compensatory gastric stretching following subtotal gastric resection due to gastric adenocarcinoma in a diamond python (Morelia spilota spilota)
HR Baron, J Šlapeta, SL Donahoe, RTJ Doneley, DN Phalen
Australian Veterinary Journal.2018; 96(12): 481. CrossRef
Parasite Infection, Carcinogenesis and Human Malignancy
Hoang van Tong, Paul J. Brindley, Christian G. Meyer, Thirumalaisamy P. Velavan
EBioMedicine.2017; 15: 12. CrossRef
Gastric Strongyloidiasis: Report of 2 Cases and Brief Review of the Literature
Rawia Mohamed, Mowafak M. Hamodat, Mousa A. Al-Abbadi
Laboratory Medicine.2017; 48(1): 93. CrossRef
Strongyloidiasis Current Status with Emphasis in Diagnosis and Drug Research
Tiago Mendes, Karen Minori, Marlene Ueta, Danilo Ciccone Miguel, Silmara Marques Allegretti
Journal of Parasitology Research.2017; 2017: 1. CrossRef
A case of adenocarcinoma developed in the small intestine with chronic strongyloidiasis
Satoshi Ishikawa, Tamaki Maeda, Kimiaki Hattori, Takahiro Watanabe, Takanori Kuramoto, Saori Ueno, Goro Ueno, Tadahiro Yamada, Akifumi Kanazawa, Masahiro Sakaguchi, Yoshitane Tsukamoto
Clinical Journal of Gastroenterology.2017; 10(6): 519. CrossRef
Colorectal Cancer Associated with Strongyloides stercoralis Colitis
Carmine Catalano, Joshua Aron, Raghav Bansal, Anatoly Leytin
ACG Case Reports Journal.2017; 4(1): e104. CrossRef
Carcinogenesis associated with parasites other than Schistosoma, Opisthorchis and Clonorchis: A systematic review
Claudia Machicado, Luis A. Marcos
International Journal of Cancer.2016; 138(12): 2915. CrossRef

16,996 View
160 Download
24 Web of Science
Crossref

Extracorporeal Worm Extraction of Diphyllobothrium nihonkaiense with Amidotrizoic Acid in a Child: Hye Kyung Shin, Joo-Hyung Roh, Jae-Won Oh, Jae-Sook Ryu, Youn-Kyoung Goo, Dong-Il Chung, Yong Joo Kim; Korean J Parasitol 2014;52(6):677-680.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.677

Abstract
PDF

Infection cases of diphyllobothriid tapeworms are not much in the below teen-age group. We report a case of Diphyllobothrium nihonkaiense infection in a 13-year-old boy. He presented with severe fatigue, occasional abdominal pain at night time. He also had several episodes of tapeworm segment discharge in his stools. By his past history, he had frequently eaten raw fish including salmon and trout with his families. Numerous eggs of diphyllobothriid tapeworm were detected in the fecal examination. We introduced amidotrizoic acid as a cathartic agent through nasogastroduodenal tube and let nearly whole length (4.75 m) of D. nihonkaiense be excreted through his anus. After a single dose of praziquantel, the child's stool showed no further eggs, and his symptoms disappeared. The evacuated worm was identified as D. nihonkaiense by mitochondrial cox1 gene analysis. Here we report a successful extracorporeal worm extraction from an infection case of D. nihonkaiense by the injection of amidotrizoic acid.

Citations

Citations to this article as recorded by

Diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from Korea
Seoyun Choi, Jaeeun Cho, Bong-Kwang Jung, Deok-Gyu Kim, Sarah Jiyoun Jeon, Hyeong-Kyu Jeon, Keeseon S. Eom, Jong-Yil Chai
Parasitology Research.2015; 114(6): 2129. CrossRef

17,083 View
115 Download
2 Web of Science
Crossref

Brief Communications

Prevalence of Trichomonas vaginalis by PCR in Men Attending a Primary Care Urology Clinic in South Korea: Jun-Hyeok Seo, Hye-Won Yang, So-Young Joo, Su-Min Song, Yu-Ran Lee, Jae-Sook Ryu, Eun Sang Yoo, Won Kee Lee, Hyun-Hee Kong, Sang-Eun Lee, Won-Ja Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(5):551-555.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.551

Abstract
PDF

Trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. Despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including PCR, few prospective studies present data on male T. vaginalis infection in South Korea. In the present study, the prevalence of T. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary care urology clinic in South Korea. The prevalence of T. vaginalis infection in our cohort was 4% (8/201) by PCR. T. vaginalis infection was common in men older than 40 years (median age, 52 years). Among the 8 Trichomonas-positive patients, 87.5% (7/8) had prostatic diseases, such as prostatitis and benign prostatic hyperplasia, and 25.0% (2/8) and 12.5% (1/8) were coinfected with Chlamydia trachomatis and Mycoplasma genitalium, respectively. Our results suggest that T. vaginalis infection is not rare in men attending primary care urology clinics in South Korea, especially in those older than 40 years, in whom it may explain the presence of prostatic disease. The possibility of T. vaginalis infection should be routinely considered in older male patients with prostatic diseases in South Korea.

Citations

Citations to this article as recorded by

Trichomoniasis in Men: A Neglected Factor in Male Infertility?
Seyed Ali Hosseini, Mohammad Matini, Maryam Bahmanzadeh, Reza Aslani, Faeze Foroughi-Parvar
Acta Parasitologica.2025;[Epub] CrossRef
Systematic review and meta-analysis of the global prevalence and infection risk factors of Trichomonas vaginalis
Wenjie Tian, Yuhua Li, Yani Zhang, Yiming Zhang, Yiran Qin, Yalin Han, Dongxian Li, Shuai Wang, Zhenke Yang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang
Parasite.2025; 32: 56. CrossRef
Prevalence of trichomoniasis infection among adults in Nigerian community settings
K. Chukwuemeka Obetta, Innocent Okonkwo Ogbonna, Dorida Nneka Oyigbo, Oliver Onyemaechi Ugwu, Kenneth Okonkwo Ugwu, Beatrice N. Onah, Chinasa Maryrose Ugwunnadi, Joseph O. Acha, Ngozi Uzoamaka Chuke, Ogechi Nkemjika, Onyinyechi Elizabeth Okoye
Medicine.2023; 102(37): e34585. CrossRef
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China
Zhenchao Zhang, Yuhui Sang, Pucheng Wu, Yujia Shang, Lesong Li, Yujuan Duan, Linfei Zhao, Minghui Gao, Lihua Guo, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lixia Hao, Xuefang Mei, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia
Ik-Hwan Han, Jung-Hyun Kim, Jae-Sook Ryu
Parasites, Hosts and Diseases.2023; 61(1): 2. CrossRef
Urogenital Trichomonas vaginalis infection in males: a case report and retrospective analysis of a 10‐year period in a tertiary hospital
Miguel Alpalhão, Luís Marques‐Lito, Paulo Filipe, João Borges‐Costa
International Journal of Dermatology.2022;[Epub] CrossRef
Seroprevalence and risk factors of Trichomonas vaginalis among couples in Al-Hamza city-Iraq.
Musafer H. Al-Ardi
Al-Kufa University Journal for Biology.2021; 13(1): 26. CrossRef
Comparison of Seropositivity to Trichomonas vaginalis between Men with Prostatic Tumor and Normal Men
Jung-Hyun Kim, Hong-Sang Moon, Kyu-Shik Kim, Hwan-Sik Hwang, Jae-Sook Ryu, Sung-Yul Park
The Korean Journal of Parasitology.2019; 57(1): 21. CrossRef
Comparison of Two PCR Assays for Trichomonas vaginalis
Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu
The Korean Journal of Parasitology.2019; 57(1): 27. CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
Prevalence of Trichomonas vaginalis in Women Visiting 2 Obstetrics and Gynecology Clinics in Daegu, South Korea
Youn-Kyoung Goo, Won-Sik Shin, Hye-Won Yang, So-Young Joo, Su-Min Song, Jae-Sook Ryu, Won-Myung Lee, Hyun-Hee Kong, Won-Ki Lee, Sang-Eun Lee, Won-Ja Lee, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2016; 54(1): 75. CrossRef
Prostatic Disease Associated withTrichomonas vaginalis
Jae-Sook Ryu
The Korean Journal of Urogenital Tract Infection and Inflammation.2014; 9(2): 61. CrossRef

11,533 View
127 Download
13 Web of Science
Crossref

Development of Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Azumiobodo hoyamushi (Kinetoplastea): Su-Min Song, Dinzouna-Boutamba Sylvatrie-Danne, So-Young Joo, Yun Kyung Shin, Hak Sun Yu, Yong-Seok Lee, Ji-Eon Jung, Noboru Inoue, Won Kee Lee, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2014;52(3):305-310.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.305

Abstract
PDF

Ascidian soft tunic syndrome (AsSTS) caused by Azumiobodo hoyamushi (A. hoyamushi) is a serious aquaculture problem that results in mass mortality of ascidians. Accordingly, the early and accurate detection of A. hoyamushi would contribute substantially to disease management and prevention of transmission. Recently, the loop-mediated isothermal amplification (LAMP) method was adopted for clinical diagnosis of a range of infectious diseases. Here, the authors describe a rapid and efficient LAMP-based method targeting the 18S rDNA gene for detection of A. hoyamushi using ascidian DNA for the diagnosis of AsSTS. A. hoyamushi LAMP assay amplified the DNA of 0.01 parasites per reaction and detected A. hoyamushi in 10 ng of ascidian DNA. To validate A. hoyamushi 18S rDNA LAMP assays, AsSTS-suspected and non-diseased ascidians were examined by microscopy, PCR, and by using the LAMP assay. When PCR was used as a gold standard, the LAMP assay showed good agreement in terms of sensitivity, positive predictive value (PPV), and negative predictive value (NPV). In the present study, a LAMP assay based on directly heat-treated samples was found to be as efficient as DNA extraction using a commercial kit for detecting A. hoyamushi. Taken together, this study shows the devised A. hoyamushi LAMP assay could be used to diagnose AsSTS in a straightforward, sensitive, and specific manner, that it could be used for forecasting, surveillance, and quarantine of AsSTS.

Citations

Citations to this article as recorded by

Measurement of Tunic Hardness in an Edible Ascidian, Halocynthia roretzi, with Remarks on Soft Tunic Syndrome
Euichi Hirose, Kei Nakayama, Tetsuya Yanagida, Akatsuki Nawata, Shin-Ichi Kitamura
Zoological Science.2018; 35(6): 548. CrossRef

9,443 View
92 Download
2 Web of Science
Crossref

Case Report

Two Human Cases of Diphyllobothrium nihonkaiense Infection in Korea: Su-Min Song, Hye-Won Yang, Min Kyu Jung, Jun Heo, Chang Min Cho, Youn-Kyoung Goo, Yeonchul Hong, Dong-Il Chung; Korean J Parasitol 2014;52(2):197-199.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.197

Abstract
PDF

Diphyllobothrium latum and Diphyllobothrium nihonkaiense are the 2 reported main causes of human diphyllobothriasis in the Republic of Korea. However, the differentiation of these 2 species based on morphologic features alone is difficult. The authors used nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene to diagnose Diphyllobothrium spp. Two patients visited the emergency room at Kyungpook National University Hospital on 3 April and 12 April 2013, respectively, with fragments of parasites found while defecating. The parasites were identified as Diphyllobothrium spp. based on morphologic characteristics, and subsequent cox1 gene sequencing showed 99.9% similarity (1,478/1,480 bp) with D. nihonkaiense. Our findings support the hypothesis that D. nihonkaiense is a dominant species in Korea.

Citations

Citations to this article as recorded by

Diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from Korea
Seoyun Choi, Jaeeun Cho, Bong-Kwang Jung, Deok-Gyu Kim, Sarah Jiyoun Jeon, Hyeong-Kyu Jeon, Keeseon S. Eom, Jong-Yil Chai
Parasitology Research.2015; 114(6): 2129. CrossRef
Three Cases of Diphyllobothrium nihonkaiense Infection in Korea
Hong-Ja Kim, Keeseon S. Eom, Min Seo
The Korean Journal of Parasitology.2014; 52(6): 673. CrossRef
Extracorporeal Worm Extraction of Diphyllobothrium nihonkaiense with Amidotrizoic Acid in a Child
Hye Kyung Shin, Joo-Hyung Roh, Jae-Won Oh, Jae-Sook Ryu, Youn-Kyoung Goo, Dong-Il Chung, Yong Joo Kim
The Korean Journal of Parasitology.2014; 52(6): 677. CrossRef

9,052 View
100 Download
6 Web of Science
Crossref

Original Articles

Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong; Korean J Parasitol 2014;52(2):131-135.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.131

Abstract
PDF

Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii
Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio
Nature Protocols.2024; 19(1): 3. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Suthinee Sangkanu, Abolghasem Siyadatpanah, Roghayeh Norouzi, Julalak Chuprom, Watcharapong Mitsuwan, Sirirat Surinkaew, Rachasak Boonhok, Alok K. Paul, Tooba Mahboob, Imran Sama-ae, Sonia M. R. Oliveira, Tajudeen O. Jimoh, Maria de Lourdes Pereira, Polra
PeerJ.2024; 12: e16937. CrossRef
Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study
Gerami D. Seitzman, Jeremy D. Keenan, Thomas M. Lietman, Kevin Ruder, Lina Zhong, Cindi Chen, YuHeng Liu, Danny Yu, Thomas Abraham, Armin Hinterwirth, Thuy Doan
Cornea.2024; 43(10): 1272. CrossRef
Evolution of giant pandoravirus revealed by CRISPR/Cas9
Hugo Bisio, Matthieu Legendre, Claire Giry, Nadege Philippe, Jean-Marie Alempic, Sandra Jeudy, Chantal Abergel
Nature Communications.2023;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens
Julalak Chuprom, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Wilawan Mahabusarakam, L. Ravithej Singh, Ekachai Dumkliang, Kritamorn Jitrangsri, Alok K. Paul, Sirirat Surinkaew, Polrat Wilairatana, Maria de Lourdes Pereira, Mohammed Rahmatu
PeerJ.2022; 10: e14468. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage
Ruixuan Zhang, Hisashi Endo, Masaharu Takemura, Hiroyuki Ogata, Samuel K. Campos
Microbiology Spectrum.2021;[Epub] CrossRef
Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector
Christopher Price, Snake Jones, Mirna Mihelcic, Marina Santic, Yousef Abu Kwaik
Cell Host & Microbe.2020; 27(4): 571. CrossRef
Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene in Acanthamoeba castellanii
Steven Rolland, Luce Mengue, Cyril Noël, Stéphanie Crapart, Anne Mercier, Willy Aucher, Yann Héchard, Ascel Samba-Louaka
Pathogens.2020; 9(5): 321. CrossRef
The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
Parasites & Vectors.2020;[Epub] CrossRef
Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture
Mária Garajová, Martin Mrva, Naděžda Vaškovicová, Michal Martinka, Janka Melicherová, Andrea Valigurová
Scientific Reports.2019;[Epub] CrossRef
The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls
Pamela Magistrado-Coxen, Yousuf Aqeel, Angelo Lopez, John R. Haserick, Breeanna R. Urbanowicz, Catherine E. Costello, John Samuelson, Photini Sinnis
PLOS Neglected Tropical Diseases.2019; 13(5): e0007352. CrossRef
A review of Acanthamoeba keratitis in the middle East and Iran
MohammadHossein Feiz Haddad, Azar Shokri, Habib Habibpour, SeyedeManizhe Heidar Neiadi
Journal of Acute Disease.2019; 8(4): 133. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii
Duo Wu, Meng Feng, Zhi-xin Wang, Ke Qiao, Hiroshi Tachibana, Xun-jia Cheng
Parasites & Vectors.2018;[Epub] CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef
In-vitro development of an effective treatment for Acanthamoeba keratitis
Á. Ortillés, J. Belloc, E. Rubio, M.T. Fernández, M. Benito, J.Á. Cristóbal, B. Calvo, P. Goñi
International Journal of Antimicrobial Agents.2017; 50(3): 325. CrossRef
Treatment of Acanthamoeba keratitis
N. R. Marchenko, Evg. A. Kasparova
Vestnik oftal'mologii.2016; 132(5): 110. CrossRef
Cellulose degradation: a therapeutic strategy in the improved treatment of Acanthamoeba infections
Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Parasites & Vectors.2015;[Epub] CrossRef
An update onAcanthamoebakeratitis: diagnosis, pathogenesis and treatment
Jacob Lorenzo-Morales, Naveed A. Khan, Julia Walochnik
Parasite.2015; 22: 10. CrossRef
Potential Value of Cellulose Synthesis Inhibitors Combined With PHMB in the Treatment of Acanthamoeba Keratitis
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Cornea.2015; 34(12): 1593. CrossRef

11,300 View
127 Download
24 Web of Science
Crossref

Identification of Atg8 Isoform in Encysting Acanthamoeba: Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2013;51(5):497-502.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.497

Abstract
PDF

Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Suthinee Sangkanu, Abolghasem Siyadatpanah, Roghayeh Norouzi, Julalak Chuprom, Watcharapong Mitsuwan, Sirirat Surinkaew, Rachasak Boonhok, Alok K. Paul, Tooba Mahboob, Imran Sama-ae, Sonia M. R. Oliveira, Tajudeen O. Jimoh, Maria de Lourdes Pereira, Polra
PeerJ.2024; 12: e16937. CrossRef
Ac-HSP20 regulates autophagy and promotes the encystation of Acanthamoeba castellanii by inhibiting the PI3K/AKT/mTOR signaling pathway
Siyao Guo, Di Liu, Xi Wan, Dingrui Guo, Meiyu Zheng, Wenyu Zheng, Xianmin Feng
Parasites & Vectors.2024;[Epub] CrossRef
Toxicity Evaluation of Potassium Sorbate In Vivo with Drosophila Melanogaster
Xubo Zhang, Qian Zhang, Xiaoxuan Song, Wanchen Yang, Andi Cheng, Jianzhen Zhang, Wei Dong
Insects.2024; 15(9): 703. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
The roles of autophagy and mitophagy in corneal pathology: current knowledge and future perspectives
Rajalakshmy Ayilam Ramachandran, Jose Marcos Sanches, Danielle M. Robertson
Frontiers in Medicine.2023;[Epub] CrossRef
Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
Rachasak Boonhok, Suthinee Sangkanu, Suganya Phumjan, Ramita Jongboonjua, Nawarat Sangnopparat, Pattamaporn Kwankaew, Aman Tedasen, Chooi Ling Lim, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Alok
PeerJ.2022; 10: e13657. CrossRef
Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasite Entamoeba histolytica
Kumiko Nakada-Tsukui, Natsuki Watanabe, Kumiko Shibata, Ratna Wahyuni, Eri Miyamoto, Tomoyoshi Nozaki
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites
Hương-Giang Lê, Ji-Su Choi, Buyng-Su Hwang, Yong-Tae Jeong, Jung-Mi Kang, Tuấn-Cường Võ, Pyo-Yun Cho, Young-Kyung Lee, Won-Gi Yoo, Yeonchul Hong, Young-Taek Oh, Byoung-Kuk Na
Plants.2022; 11(24): 3459. CrossRef
Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel
Parasitology.2021; 148(9): 1074. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Ubiquitin-Like Modifiers: Emerging Regulators of Protozoan Parasites
Maryia Karpiyevich, Katerina Artavanis-Tsakonas
Biomolecules.2020; 10(10): 1403. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Autophagy protein 12 plays an essential role in Acanthamoeba encystation
So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Experimental Parasitology.2015; 159: 46. CrossRef
Autophagy Inhibitors as a Potential Antiamoebic Treatment for Acanthamoeba Keratitis
Eun-Kyung Moon, So-Hee Kim, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Antimicrobial Agents and Chemotherapy.2015; 59(7): 4020. CrossRef

9,819 View
99 Download
Crossref

Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba: Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2013;51(3):269-277.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.269

Abstract
PDF

Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.

Citations

Citations to this article as recorded by

Ultrasensitive and rapid diagnostic tool for detection of Acanthamoeba castellanii
Susanna Haapanen, Maarit S. Patrikainen, Seppo Parkkila
Diagnostic Microbiology and Infectious Disease.2023; 107(2): 116014. CrossRef
A simple and visible detection method for the rapid diagnosis of Ustilaginoidea virens in rice seeds by a loop‐mediated isothermal amplification assay
Wei Wang, Hang Yin, Ning Huang, Cuijing Zhu, Yufei Wang, Xintong Qi, Lu Ma, Yunxin Fan, Yao Yu, Hongsheng Zhang, Yongmei Bao
Journal of Phytopathology.2021; 169(6): 369. CrossRef
Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample
Tsui-Kang Hsu, Jung-Sheng Chen, Hsin-Chi Tsai, Chi-Wei Tao, Yu-Yin Yang, Ying-Chin Tseng, Yi-Jie Kuo, Dar-Der Ji, Jagat Rathod, Bing-Mu Hsu
Scientific Reports.2021;[Epub] CrossRef
Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis of Plasmodium vivax Malaria in a Tertiary Hospital in Chandigarh, North India
Hargobinder Kaur, Rakesh Sehgal, Devendra Bansal, Ali A. Sultan, Ashish Bhalla, Sunit C. Singhi
The American Journal of Tropical Medicine and Hygiene.2018; 98(5): 1374. CrossRef
Detection of Acanthamoeba spp. in water samples collected from natural water reservoirs, sewages, and pharmaceutical factory drains using LAMP and PCR in China
Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
Science of The Total Environment.2017; 584-585: 489. CrossRef
Water-borne protozoa parasites: The Latin American perspective
Félix Manuel Rosado-García, Milena Guerrero-Flórez, Gabriele Karanis, María Del Carmen Hinojosa, Panagiotis Karanis
International Journal of Hygiene and Environmental Health.2017; 220(5): 783. CrossRef
Evaluation of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Acanthamoeba Keratitis
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
Indian Journal of Medical Microbiology.2017; 35(1): 90. CrossRef
Acanthamoeba keratitis: improving the Scottish diagnostic service for the rapid molecular detection of Acanthamoeba species
Claire Low Alexander, Michael Coyne, Brian Jones, Deepa Anijeet
Journal of Medical Microbiology .2015; 64(7): 682. CrossRef
Molecular diagnosis in clinical parasitology: When and why?
Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
Experimental Biology and Medicine.2014; 239(11): 1443. CrossRef

11,275 View
96 Download
Crossref

Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii: Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong; Korean J Parasitol 2011;49(4):341-347.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.341

Abstract
PDF

Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of Acanthamoeba, we compared the transcription profile between cysts and trophozoites using microarray analysis. The DNA chip was composed of 12,544 genes based on expressed sequence tag (EST) from an Acanthamoeba ESTs database (DB) constructed in our laboratory, genetic information of Acanthamoeba from TBest DB, and all of Acanthamoeba related genes registered in the NCBI. Microarray analysis indicated that 701 genes showed higher expression than 2 folds in cysts than in trophozoites, and 859 genes were less expressed in cysts than in trophozoites. The results of real-time PCR analysis of randomly selected 9 genes of which expression was increased during cyst formation were coincided well with the microarray results. Eukaryotic orthologous groups (KOG) analysis showed an increment in T article (signal transduction mechanisms) and O article (posttranslational modification, protein turnover, and chaperones) whereas significant decrement of C article (energy production and conversion) during cyst formation. Especially, cystein proteinases showed high expression changes (282 folds) with significant increases in real-time PCR, suggesting a pivotal role of this proteinase in the cyst formation of Acanthamoeba. The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba.

Citations

Citations to this article as recorded by

DNA methylation modification: Dawn of research on cornea-related diseases
Quanhao Pan, Xiaoning Ge, Di Wang, Yuxi He
Life Sciences.2025; 376: 123757. CrossRef
Identification and characterization of a DNA-binding protein from starved cells (Dps) homologue in Acanthamoeba: Implications for encystment-induced DNA protection
Seunghyeok Bang, So-Young Joo, Ja Moon Aung, Je Chul Lee, Byoung-Kuk Na, Yeonchul Hong, Minsang Shin
Acta Tropica.2025; 271: 107837. CrossRef
Microarray-based characterization of airway inflammation induced by environmental Acanthamoeba exposure versus the ovalbumin-alum model
Shin Ae Kang, Hak Sun Yu
Parasites, Hosts and Diseases.2025; 63(4): 327. CrossRef
Proteases of Acanthamoeba
Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani
Parasitology Research.2024;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
mRNA Sequencing Reveals Upregulation of Glutathione S-Transferase Genes during Acanthamoeba Encystation
Alvaro de Obeso Fernández del Valle, Christian Quintus Scheckhuber, David Armando Chavaro-Pérez, Erandi Ortega-Barragán, Sutherland K. Maciver
Microorganisms.2023; 11(4): 992. CrossRef
Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram
Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver
Processes.2023; 11(9): 2620. CrossRef
Comparative analysis of differentially expressed genes in Acanthamoeba after ingestion of Legionella pneumophila and Escherichia coli
Eun-Kyung Moon, Min-Jeong Kim, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2022; 232: 108188. CrossRef
Anaerobic ATP synthesis pathways and inorganic phosphate transport and their possible roles in encystment in Acanthamoeba castellanii
Luiz Fernando Carvalho‐Kelly, Claudia Fernanda Dick, Nathalia Rocco‐Machado, André Luiz Gomes‐Vieira, Lisvane Paes‐Vieira, José Roberto Meyer‐Fernandes
Cell Biology International.2022; 46(8): 1288. CrossRef
Acanthamoeba castellanii exhibits intron retention during encystment
Alvaro de Obeso Fernández del Valle, Jesús Gómez-Montalvo, Sutherland K. Maciver
Parasitology Research.2022; 121(9): 2615. CrossRef
A Novel Cysteine Protease Inhibitor of Naegleria fowleri That Is Specifically Expressed during Encystation and at Mature Cysts
Hương Giang Lê, A-Jeong Ham, Jung-Mi Kang, Tuấn Cường Võ, Haung Naw, Hae-Jin Sohn, Ho-Joon Shin, Byoung-Kuk Na
Pathogens.2021; 10(4): 388. CrossRef
The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
Parasites & Vectors.2020;[Epub] CrossRef
Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts
Hae-Jin Sohn, Ga-Eun Seo, Jae-Ho Lee, A-Jeong Ham, Young-Hwan Oh, Heekyoung Kang, Ho-Joon Shin
The Korean Journal of Parasitology.2019; 57(3): 217. CrossRef
Soil protists: a fertile frontier in soil biology research
Stefan Geisen, Edward A D Mitchell, Sina Adl, Michael Bonkowski, Micah Dunthorn, Flemming Ekelund, Leonardo D Fernández, Alexandre Jousset, Valentyna Krashevska, David Singer, Frederick W Spiegel, Julia Walochnik, Enrique Lara
FEMS Microbiology Reviews.2018; 42(3): 293. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Eun-Kyung Moon, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2017; 55(2): 115. CrossRef
Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii
Eun-Kyung Moon, Hyun-Hee Kong, Yeonchul Hong, Hae-Ahm Lee, Fu-Shi Quan
The Korean Journal of Parasitology.2017; 55(2): 109. CrossRef
Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii
Yu-Ran Lee, Byoung-Kuk Na, Eun-Kyung Moon, Su-Min Song, So-Young Joo, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong, Patricia Talamas-Rohana
PLOS ONE.2015; 10(6): e0129884. CrossRef
Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
The Korean Journal of Parasitology.2014; 52(2): 131. CrossRef
Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage
Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong
Experimental Parasitology.2014; 143: 69. CrossRef
Proteomic profiling of the infective trophozoite stage of Acanthamoeba polyphaga
Karin Silva Caumo, Karina Mariante Monteiro, Thiely Rodrigues Ott, Vinicius José Maschio, Glauber Wagner, Henrique Bunselmeyer Ferreira, Marilise Brittes Rott
Acta Tropica.2014; 140: 166. CrossRef
Cysteine Protease Inhibitor (AcStefin) Is Required for Complete Cyst Formation of Acanthamoeba
Jung-Yub Lee, Su-Min Song, Eun-Kyung Moon, Yu-Ran Lee, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hee-Jae Cha, Hak Sun Yu, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong
Eukaryotic Cell.2013; 12(4): 567. CrossRef
Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef
Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba
Eun-Kyung Moon, Hyun-Hee Kong
The Korean Journal of Parasitology.2012; 50(4): 361. CrossRef
Protein kinase C signaling molecules regulate encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
Experimental Parasitology.2012; 132(4): 524. CrossRef

9,624 View
96 Download
Crossref

Expressed Sequence Tag Analysis of the Erythrocytic Stage of Plasmodium berghei: Ji-Woong Seok, Yong-Seok Lee, Eun-Kyung Moon, Jung-Yub Lee, Bijay Kumar Jha, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong; Korean J Parasitol 2011;49(3):221-228.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.221

Abstract
PDF

Rodent malaria parasites, such as Plasmodium berghei, are practical and useful model organisms for human malaria research because of their analogies to the human malaria in terms of structure, physiology, and life cycle. Exploiting the available genetic sequence information, we constructed a cDNA library from the erythrocytic stages of P. berghei and analyzed the expressed sequence tag (EST). A total of 10,040 ESTs were generated and assembled into 2,462 clusters. These EST clusters were compared against public protein databases and 48 putative new transcripts, most of which were hypothetical proteins with unknown function, were identified. Genes encoding ribosomal or membrane proteins and purine nucleotide phosphorylases were highly abundant clusters in P. berghei. Protein domain analyses and the Gene Ontology functional categorization revealed translation/protein folding, metabolism, protein degradation, and multiple family of variant antigens to be mainly prevalent. The presently-collected ESTs and its bioinformatic analysis will be useful resources to identify for drug target and vaccine candidates and validate gene predictions of P. berghei.

9,140 View
59 Download

Atg3-Mediated Lipidation of Atg8 Is Involved in Encystation of Acanthamoeba: Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong; Korean J Parasitol 2011;49(2):103-108.
Published online June 14, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.2.103

Abstract
PDF

Autophagy is a catabolic process involved in the degradation of a cell's own components for cell growth, development, homeostasis, and the recycling of cellular products. Autophagosome is an essential component in the protozoan parasite during differentiation and encystation. The present study identified and characterized autophagy-related protein (Atg) 3, a member of Atg8 conjugation system, in Acanthamoeba castellanii (AcAtg3). AcAtg3 encoding a 304 amino acid protein showed high similarity with the catalytic cysteine site of other E2 like enzymes of ubiquitin system. Predicted 3D structure of AcAtg3 revealed a hammer-like shape, which is the characteristic structure of E2-like enzymes. The expression level of AcAtg3 did not increase during encystation. However, the formation of mature cysts was significantly reduced in Atg3-siRNA transfected cells in which the production of Atg8-phosphatidylethanolamine conjugate was inhibited. Fluorescent microscopic analysis revealed that dispersed AcAtg3-EGFP fusion protein gathered around autophagosomal membranes during encystation. These results provide important information for understanding autophagic machinery through the lipidation reaction mediated by Atg3 in Acanthamoeba.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Ac-HSP20 regulates autophagy and promotes the encystation of Acanthamoeba castellanii by inhibiting the PI3K/AKT/mTOR signaling pathway
Siyao Guo, Di Liu, Xi Wan, Dingrui Guo, Meiyu Zheng, Wenyu Zheng, Xianmin Feng
Parasites & Vectors.2024;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
The roles of autophagy and mitophagy in corneal pathology: current knowledge and future perspectives
Rajalakshmy Ayilam Ramachandran, Jose Marcos Sanches, Danielle M. Robertson
Frontiers in Medicine.2023;[Epub] CrossRef
Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae
Ascel Samba-Louaka, Elisabeth Labruyère, Mariette Matondo, Marie Locard-Paulet, Jean-Christophe Olivo-Marin, Nancy Guillen
Microorganisms.2023; 11(11): 2670. CrossRef
Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
Rachasak Boonhok, Suthinee Sangkanu, Suganya Phumjan, Ramita Jongboonjua, Nawarat Sangnopparat, Pattamaporn Kwankaew, Aman Tedasen, Chooi Ling Lim, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Karma G. Dolma, Alok
PeerJ.2022; 10: e13657. CrossRef
A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment
Clément Bernard, Marie Locard-Paulet, Cyril Noël, Magalie Duchateau, Quentin Giai Gianetto, Bouziane Moumen, Thomas Rattei, Yann Hechard, Lars Juhl Jensen, Mariette Matondo, Ascel Samba-Louaka
Nature Communications.2022;[Epub] CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites
Hương-Giang Lê, Ji-Su Choi, Buyng-Su Hwang, Yong-Tae Jeong, Jung-Mi Kang, Tuấn-Cường Võ, Pyo-Yun Cho, Young-Kyung Lee, Won-Gi Yoo, Yeonchul Hong, Young-Taek Oh, Byoung-Kuk Na
Plants.2022; 11(24): 3459. CrossRef
Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel
Parasitology.2021; 148(9): 1074. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Identification of Autophagy-related Protein 3 in the Ancient Protist Trichomonas vaginalis
Chang-Huei Tsao, Hsin-An Lin, Hsin-Chung Lin, Ruei-Min Chen, Chien-Fu F. Chen, Yu-Chun Lin, Kuo-Yang Huang
Journal of Medical Sciences.2021; 41(1): 1. CrossRef
Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation
Areeba Anwar, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Biology.2020; 9(4): 79. CrossRef
Transcriptomic changes across the life cycle of Trypanosoma cruzi II
Lissa Cruz-Saavedra, Gustavo A. Vallejo, Felipe Guhl, Juan David Ramírez
PeerJ.2020; 8: e8947. CrossRef
The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba
So-Young Joo, Ja Moon Aung, Minsang Shin, Eun-Kyung Moon, Hyun-Hee Kong, Youn-Kyoung Goo, Dong-Il Chung, Yeonchul Hong
Parasites & Vectors.2020;[Epub] CrossRef
Apoptosis of Acanthamoeba castellanii Trophozoites Induced by Oleic Acid
Duo Wu, Ke Qiao, Meng Feng, Yongfeng Fu, Junlong Cai, Yihong Deng, Hiroshi Tachibana, Xunjia Cheng
Journal of Eukaryotic Microbiology.2018; 65(2): 191. CrossRef
Identification and ultrastructural characterization of Acanthamoeba bacterial endocytobionts belonging to the Alphaproteobacteria class
Li Li Chan, Joon Wah Mak, Stephen Ambu, Pei Yee Chong, Lorenzo Brusetti
PLOS ONE.2018; 13(10): e0204732. CrossRef
Atg8 is involved in endosomal and phagosomal acidification in the parasitic protist E ntamoeba histolytica
Karina Picazarri, Kumiko Nakada‐Tsukui, Kumiko Tsuboi, Eri Miyamoto, Naoko Watanabe, Eiryo Kawakami, Tomoyoshi Nozaki
Cellular Microbiology.2015; 17(10): 1510. CrossRef
Autophagy protein 12 plays an essential role in Acanthamoeba encystation
So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Experimental Parasitology.2015; 159: 46. CrossRef
Autophagy Inhibitors as a Potential Antiamoebic Treatment for Acanthamoeba Keratitis
Eun-Kyung Moon, So-Hee Kim, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
Antimicrobial Agents and Chemotherapy.2015; 59(7): 4020. CrossRef
Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy
Bijay Kumar Jha, Hui-Jung Jung, Incheol Seo, Hyun Ah Kim, Seong-Il Suh, Min-Ho Suh, Won-Ki Baek
Antimicrobial Agents and Chemotherapy.2014; 58(10): 6235. CrossRef
Identification of Atg8 Isoform in Encysting Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2013; 51(5): 497. CrossRef
Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef
Protein kinase C signaling molecules regulate encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
Experimental Parasitology.2012; 132(4): 524. CrossRef
Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites ofAcanthamoeba castellanii
Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
The Korean Journal of Parasitology.2011; 49(4): 341. CrossRef

10,130 View
94 Download
Crossref

Construction of EST Database for Comparative Gene Studies of Acanthamoeba: Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2009;47(2):103-107.
Published online May 26, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.103

Abstract
PDF

The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported cDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http://www.amoeba.or.kr).

Citations

Citations to this article as recorded by

Recombinant protein expression in Acanthamoeba castellanii
Pooja Salunke, Kiran Kondabagil, Yogesh A. Karpe
Frontiers in Bioengineering and Biotechnology.2025;[Epub] CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Autophagy protein 12 plays an essential role in Acanthamoeba encystation
So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Experimental Parasitology.2015; 159: 46. CrossRef
Caspase-like proteins: Acanthamoeba castellanii metacaspase and Dictyostelium discoideum paracaspase, what are their functions?
Entsar Saheb, Wendy Trzyna, John Bush
Journal of Biosciences.2014; 39(5): 909. CrossRef
Helminth parasites of fish and shellfish from the Santa Gilla Lagoon in southern Sardinia, Italy
J. Culurgioni, A. Sabatini, R. De Murtas, S. Mattiucci, V. Figus
Journal of Helminthology.2014; 88(04): 489. CrossRef
Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage
Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong
Experimental Parasitology.2014; 143: 69. CrossRef
Protein kinase C signaling molecules regulate encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
Experimental Parasitology.2012; 132(4): 524. CrossRef
Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites ofAcanthamoeba castellanii
Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
The Korean Journal of Parasitology.2011; 49(4): 341. CrossRef
Drug target identification, validation, characterisation and exploitation for treatment of Acanthamoeba (species) infections
Craig W. Roberts, Fiona L. Henriquez
Experimental Parasitology.2010; 126(1): 91. CrossRef
Construction of Web-Based Database for Anisakis Research
Yong-Seok Lee, Moon-Ki Baek, Yong-Hun Jo, Se-Won Kang, Jae-Bong Lee, Yeon-Soo Han, Hee-Jae Cha, Hak-Sun Yu, Mee-Sun Ock
Journal of Life Science.2010; 20(3): 411. CrossRef

10,227 View
59 Download
Crossref

Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates: Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung; Korean J Parasitol 2008;46(3):157-164.
Published online September 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.3.157

Abstract
PDF

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a corneal pathogen. Epidemiologic significance of A. triangularis as a keratopathogen in Korea has been discussed. Morphologic features of Acanthamoeba cysts were examined under a microscope with differential interference contrast (DIC) optics. Mitochondrial DNA (mtDNA) of the ocular isolates KA/E9, KA/E17, and KA/E23 were digested with restriction enzymes, and the restriction patterns were compared with those of reference strains. Complete nuclear 18S and mitochondrial (mt) 16S rDNA sequences were subjected to phylogenetic analysis and species identification. mtDNA RFLP of 3 isolates showed very similar patterns to those of SH621, the type strain of A. triangularis. 16S and 18S rDNA sequence analysis confirmed 3 isolates to be A. triangularis. 18S rDNA sequence differences of the isolates were 1.3% to 1.6% and those of 16S rDNA, 0.4% to 0.9% from A. triangularis SH621. To the best of our knowledge, this is the first report, confirmed by 18S and 16S rDNA sequence analysis, of keratitis caused by A. triangularis of which the type strain was isolated from human feces. Six isolates of A. triangularis had been reported from contaminated contact lens cases in south eastern Korea.

Citations

Citations to this article as recorded by

Inhibitory and anti-adherent effects of Piper betle L. leaf extract against Acanthamoeba triangularis in co-infection with Staphylococcus aureus and Pseudomonas aeruginosa: A sustainable one-health approach
Pattamaporn Kwankaew, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Udom Lao-On, Hazel L. Tabo, Tooba Mahboob, Maria de Lourdes Pereira, Jitbanjong Tangpong, Shanmuga S. Sundar, Christophe Wiart, Veeranoot Nissapatorn
Veterinary World.2024; : 848. CrossRef
The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Paul A. Fuerst
Microorganisms.2023; 11(2): 295. CrossRef
Sub-Genotyping of Acanthamoeba T4 Complex: Experience from North India
Kirti Megha, Megha Sharma, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Parasitologia.2023; 3(1): 69. CrossRef
The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea
Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim
Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef
Detection of Acanthamoeba spp. using carboxylesterase antibody and its usage for diagnosing Acanthamoeba-keratitis
Min-Jeong Kim, Ki-Back Chu, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon, Ashok Kumar
PLOS ONE.2022; 17(1): e0262223. CrossRef
Report of rare genotypes of Acanthamoeba from soil source of the Payeh Maga Highland forest, North-eastern Sarawak, Malaysia
Li-Li Chan, Hiu-Ching Toh, Kantiya Jaikwang, Ee-Mun Loo, Jia-Haw Wong, Yun-Khoon Liew, Kian-Huat Ong, Soo-Shen Ooi
Acta Tropica.2022; 229: 106372. CrossRef
Isolation and morphological and molecular characterization of waterborne free-living amoebae: Evidence of potentially pathogenic Acanthamoeba and Vahlkampfiidae in Assiut, Upper Egypt
Martina M. Nageeb, Hanan E. M. Eldeek, Rasha A. H. Attia, Atef A. Sakla, Samia S. Alkhalil, Haiam Mohamed Mahmoud Farrag, Ebrahim Shokoohi
PLOS ONE.2022; 17(7): e0267591. CrossRef
An Insight into the Genome of Pathogenic and Non-Pathogenic Acanthamoeba
Chayan Sharma, Sumeeta Khurana, Amit Arora, Alka Bhatia, Amit Gupta
Pathogens.2022; 11(12): 1558. CrossRef
Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review
Jinfang Wu, Huatao Xie
Journal of International Medical Research.2021;[Epub] CrossRef
Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms
Suthinee Sangkanu, Watcharapong Mitsuwan, Wilawan Mahabusarakam, Tajudeen O. Jimoh, Polrat Wilairatana, Ana Paula Girol, Ajoy K. Verma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Abolghasem Siyadatpanah, Roghayeh Norouzi, Polydor Ng
Scientific Reports.2021;[Epub] CrossRef
Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level
Rachasak Boonhok, Suthinee Sangkanu, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Nurdina Charong, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Hazel
Parasitology.2021; 148(9): 1074. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Molecular detection of free-living amoebae from Namhangang (southern Han River) in Korea
Heekyoung Kang, Hae-Jin Sohn, Ga-Eun Seo, Gi-Sang Seong, A-Jeong Ham, A-Young Park, Suk-Yul Jung, Sang-Eun Lee, Shin-Hyeong Cho, Ho-Joon Shin
Scientific Reports.2020;[Epub] CrossRef
Description of Virulent Factors and Horizontal Gene Transfers of Keratitis-Associated Amoeba Acanthamoeba Triangularis by Genome Analysis
Issam Hasni, Julien Andréani, Philippe Colson, Bernard La Scola
Pathogens.2020; 9(3): 217. CrossRef
Update on Acanthamoeba phylogeny
Daniele Corsaro
Parasitology Research.2020; 119(10): 3327. CrossRef
Production of a polyclonal antibody against inosine-uridine preferring nucleoside hydrolase of Acanthamoeba castellanii and its access to diagnosis of Acanthamoeba keratitis
So-Min Park, Hae-Ahm Lee, Ki-Back Chu, Fu-Shi Quan, Su-Jung Kim, Eun-Kyung Moon, Paulo Lee Ho
PLOS ONE.2020; 15(9): e0239867. CrossRef
Review of Successful Control of Parasitic Infections in Korea
Sung-Tae Hong, Tai-Soon Yong
Infection & Chemotherapy.2020; 52(3): 427. CrossRef
The Spectrum of Microbial Keratitis: An Updated Review
Christopher Bartimote, John Foster, Stephanie Watson
The Open Ophthalmology Journal.2019; 13(1): 100. CrossRef
Pathogenic waterborne free-living amoebae: An update from selected Southeast Asian countries
Mohamad Azlan Abdul Majid, Tooba Mahboob, Brandon G. J. Mong, Narong Jaturas, Reena Leeba Richard, Tan Tian-Chye, Anusorn Phimphila, Panomphanh Mahaphonh, Kyaw Nyein Aye, Wai Lynn Aung, Joon Chuah, Alan D. Ziegler, Atipat Yasiri, Nongyao Sawangjaroen, Yvo
PLOS ONE.2017; 12(2): e0169448. CrossRef
Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China
Yinghua Xuan, Yanqin Shen, Yuxi Ge, Gen Yan, Shanzi Zheng
Environmental Health and Preventive Medicine.2017;[Epub] CrossRef
Acanthamoebicidal activity of periglaucine A and betulinic acid from Pericampylus glaucus (Lam.) Merr. in vitro
Tooba Mahboob, Abdul-Majid Azlan, Fiona Natalia Shipton, Patcharaporn Boonroumkaew, Nadiah Syafiqah Nor Azman, Shamala Devi Sekaran, Init Ithoi, Tian-Chye Tan, Chandramathi Samudi, Christophe Wiart, Veeranoot Nissapatorn
Experimental Parasitology.2017; 183: 160. CrossRef
Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent chlorogenic acid in vitro
Tooba Mahboob, Abdul-Majid Azlan, Tian-Chye Tan, Chandramathi Samudi, Shamala Devi Sekaran, Veeranoot Nissapatorn, Christophe Wiart
Asian Pacific Journal of Tropical Medicine.2016; 9(9): 866. CrossRef
First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans
Anna-Lena Grün, Birthe Stemplewitz, Patrick Scheid
Parasitology Research.2014; 113(6): 2395. CrossRef
Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba
Hye-Won Yang, Yu-Ran Lee, Noboru Inoue, Bijay Kumar Jha, Dinzouna-Boutamba Sylvatrie Danne, Hong-Kyun Kim, Junhun Lee, Youn-Kyoung Goo, Hyun-Hee Kong, Dong-Il Chung, Yeonchul Hong
The Korean Journal of Parasitology.2013; 51(3): 269. CrossRef
Imported Intraocular Gnathostomiasis with Subretinal Tracks Confirmed by Western Blot Assay
Ji Ho Yang, Moosang Kim, Eung Suk Kim, Byoung-Kuk Na, Seung-Young Yu, Hyung-Woo Kwak
The Korean Journal of Parasitology.2012; 50(1): 73. CrossRef
Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia
Viera Nagyová, Arpád Nagy, Jozef Timko
Parasitology Research.2010; 106(4): 861. CrossRef
Acanthamoeba strains show reduced temperature tolerance after long-term axenic culture
Wilawan Pumidonming, Martina Koehsler, Julia Walochnik
Parasitology Research.2010; 106(3): 553. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef

10,072 View
87 Download
Crossref

Brief Communication

Differentially expressed genes of Acanthamoeba castellanii during encystation: Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong; Korean J Parasitol 2007;45(4):283-285.
Published online December 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.4.283

Abstract
PDF

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.

Citations

Citations to this article as recorded by

Encystment and Excystment Processes in Acanthamoeba castellanii: An Emphasis on Cellulose Involvement
Mathew Choaji, Ascel Samba-Louaka, Zineb Fechtali-Moute, Willy Aucher, Sébastien Pomel
Pathogens.2025; 14(3): 268. CrossRef
Oxidase enzyme genes are differentially expressed during Acanthamoeba castellanii encystment
Christian Q. Scheckhuber, Rebeca Damián Ferrara, Jesús Gómez-Montalvo, Sutherland K. Maciver, Alvaro de Obeso Fernández del Valle
Parasitology Research.2024;[Epub] CrossRef
Ouabain, ATPase inhibitor, potentially enhances the effect of polyhexamethylene biguanide on Acanthamoeba castellanii
Kuang-Yi Shih, Yao-Tsung Chang, Yu-Jen Wang, Jian-Ming Huang
International Journal for Parasitology: Drugs and Drug Resistance.2024; 25: 100550. CrossRef
Acanthamoeba keratitis: new hopes for potential interventions for a curable but often refractory disease
Bader Saleem Alawfi, Naveed Ahmed Khan, David Lloyd, Ruqaiyyah Siddiqui
Expert Review of Ophthalmology.2024; 19(4): 271. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
mRNA Sequencing Reveals Upregulation of Glutathione S-Transferase Genes during Acanthamoeba Encystation
Alvaro de Obeso Fernández del Valle, Christian Quintus Scheckhuber, David Armando Chavaro-Pérez, Erandi Ortega-Barragán, Sutherland K. Maciver
Microorganisms.2023; 11(4): 992. CrossRef
Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth
Zineb Fechtali-Moute, Philippe M. Loiseau, Sébastien Pomel
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation
Areeba Anwar, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Biology.2020; 9(4): 79. CrossRef
New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy
Fernando Pérez-Cota, Richard J. Smith, Hany M. Elsheikha, Matt Clark
Biomedical Optics Express.2019; 10(5): 2399. CrossRef
Encystation: the most prevalent and underinvestigated differentiation pathway of eukaryotes
Pauline Schaap, Christina Schilde
Microbiology.2018; 164(5): 727. CrossRef
Acanthamoeba and mimivirus interactions: the role of amoebal encystment and the expansion of the ‘Cheshire Cat’ theory
Ludmila Karen dos Santos Silva, Paulo Victor Miranda Boratto, Bernard La Scola, Cláudio Antônio Bonjardim, Jônatas Santos Abrahão
Current Opinion in Microbiology.2016; 31: 9. CrossRef
Autophagy protein 12 plays an essential role in Acanthamoeba encystation
So-Hee Kim, Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Experimental Parasitology.2015; 159: 46. CrossRef
Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong
The Korean Journal of Parasitology.2014; 52(2): 131. CrossRef
Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy
Bijay Kumar Jha, Hui-Jung Jung, Incheol Seo, Hyun Ah Kim, Seong-Il Suh, Min-Ho Suh, Won-Ki Baek
Antimicrobial Agents and Chemotherapy.2014; 58(10): 6235. CrossRef
Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii
Yousuf Aqeel, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Parasitology Research.2013; 112(3): 1221. CrossRef
Acanthamoebadifferentiation: a two-faced drama ofDr Jekyll and Mr Hyde
RUQAIYYAH SIDDIQUI, RICKY DUDLEY, NAVEED AHMED KHAN
Parasitology.2012; 139(7): 826. CrossRef
Protein kinase C signaling molecules regulate encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
Experimental Parasitology.2012; 132(4): 524. CrossRef
Cellular, Biochemical, and Molecular Changes during Encystment of Free-Living Amoebae
Emilie Fouque, Marie-Cécile Trouilhé, Vincent Thomas, Philippe Hartemann, Marie-Hélène Rodier, Yann Héchard
Eukaryotic Cell.2012; 11(4): 382. CrossRef
Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites ofAcanthamoeba castellanii
Eun-Kyung Moon, Ying-Hua Xuan, Dong-Il Chung, Yeonchul Hong, Hyun-Hee Kong
The Korean Journal of Parasitology.2011; 49(4): 341. CrossRef
Major Role for Cysteine Proteases during the Early Phase of Acanthamoeba castellanii Encystment
David Leitsch, Martina Köhsler, Martina Marchetti-Deschmann, Andrea Deutsch, Günter Allmaier, Michael Duchêne, Julia Walochnik
Eukaryotic Cell.2010; 9(4): 611. CrossRef
Acanthamoeba castellanii: Proteins involved in actin dynamics, glycolysis, and proteolysis are regulated during encystation
Sabrina Bouyer, Marie-Hélène Rodier, Alain Guillot, Yann Héchard
Experimental Parasitology.2009; 123(1): 90. CrossRef
Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong
Eukaryotic Cell.2008; 7(9): 1513. CrossRef

8,341 View
123 Download
Crossref

Original Articles

Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case: Hak Sun Yu, Hae Jin Jeong, Yeon-Chul Hong, Seong-Yong Seol, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2007;45(1):11-18.
Published online March 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.1.11

Abstract
PDF

Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within free-living amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired from a contact lens storage case. The bacterium multiplied within Acanthamoeba, but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. Trasnmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba. On the basis of the results of 18S rRNA gene analysis, the amoeba was identified as A. lugdunensis. A 16S rRNA gene analysis placed this bacterium within the genus Mycobacterium. The bacterium evidenced positive reactivity for acid-fast and fluorescent acid-fast stains. The bacterium was capable of growth on the Middlebrook 7H11-Mycobacterium-specific agar. The identification and characterization of bacterial endosymbionts of free-living protozoa bears significant implications for our understanding of the ecology and the identification of other atypical mycobacterial pathogens.

Citations

Citations to this article as recorded by

A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates
Xiaobin Gu, Xiuhai Lu, Shudan Lin, Xinrui Shi, Yue Shen, Qingsong Lu, Yiying Yang, Jing Yang, Jiabei Cai, Chunyan Fu, Yongliang Lou, Meiqin Zheng, Tim Downing, Kirti Megha
Microbiology Spectrum.2022;[Epub] CrossRef
A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
Pathogens.2021; 10(2): 225. CrossRef
Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa
Clarissa van der Loo, Catheleen Bartie, Tobias George Barnard, Natasha Potgieter
International Journal of Environmental Research and Public Health.2021; 18(8): 3912. CrossRef
The key factors contributing to the risk, diagnosis and treatment of non-tuberculous mycobacterial opportunistic infections
Anna Grzegorzewicz, Mariola Paściak
Postępy Higieny i Medycyny Doświadczalnej.2021; 75(1): 696. CrossRef
Co-Existence of Free-Living Amoebae and Potential Human Pathogenic Bacteria Isolated from Rural Household Water Storage Containers
Natasha Potgieter, Clarissa van der Loo, Tobias George Barnard
Biology.2021; 10(12): 1228. CrossRef
The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment
Olivia Lanzoni, Andrey Plotnikov, Yuri Khlopko, Giulio Munz, Giulio Petroni, Alexey Potekhin
Scientific Reports.2019;[Epub] CrossRef
Repertoire of free-living protozoa in contact lens solutions
Ibtissem Bouchoucha, Aurore Aziz, Louis Hoffart, Michel Drancourt
BMC Ophthalmology.2016;[Epub] CrossRef
Current and Past Strategies for Bacterial Culture in Clinical Microbiology
Jean-Christophe Lagier, Sophie Edouard, Isabelle Pagnier, Oleg Mediannikov, Michel Drancourt, Didier Raoult
Clinical Microbiology Reviews.2015; 28(1): 208. CrossRef
Looking in amoebae as a source of mycobacteria
M. Drancourt
Microbial Pathogenesis.2014; 77: 119. CrossRef
Molecular characterization and ultrastructure of a new amoeba endoparasite belonging to the Stenotrophomonas maltophilia complex
Daniele Corsaro, Karl-Dieter Müller, Rolf Michel
Experimental Parasitology.2013; 133(4): 383. CrossRef
Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques
Alicia Garcia, Pilar Goñi, Joanna Cieloszyk, Maria Teresa Fernandez, Laura Calvo-Beguería, Encarnacion Rubio, Maria Francisca Fillat, Maria Luisa Peleato, Antonio Clavel
Environmental Science & Technology.2013; 47(7): 3132. CrossRef
Cooccurrence of Free-Living Amoebae and Nontuberculous Mycobacteria in Hospital Water Networks, and Preferential Growth of Mycobacterium avium in Acanthamoeba lenticulata
Alida R. Ovrutsky, Edward D. Chan, Marinka Kartalija, Xiyuan Bai, Mary Jackson, Sara Gibbs, Joseph O. Falkinham, Michael D. Iseman, Paul R. Reynolds, Gerald McDonnell, Vincent Thomas
Applied and Environmental Microbiology.2013; 79(10): 3185. CrossRef
Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates
Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso
Ophthalmology.2010; 117(3): 445. CrossRef
Biodiversity of amoebae and amoeba-associated bacteria in water treatment plants
Daniele Corsaro, Gemma Saucedo Pages, Vicente Catalan, Jean-François Loret, Gilbert Greub
International Journal of Hygiene and Environmental Health.2010; 213(3): 158. CrossRef
Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard
FEMS Microbiology Reviews.2010; 34(3): 231. CrossRef
Free-living amoebae, a training field for macrophage resistance of mycobacteria
I.B. Salah, E. Ghigo, M. Drancourt
Clinical Microbiology and Infection.2009; 15(10): 894. CrossRef
Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea
Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu
The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef
Survival of amoebae on building materials
T. Yli-Pirilä, J. Kusnetsov, M.-R. Hirvonen, M. Seuri, A. Nevalainen
Indoor Air.2009; 19(2): 113. CrossRef
Occurrence of Free-Living Amoebae in Communities of Low and High Endemicity for Buruli Ulcer in Southern Benin
Miriam Eddyani, Johan F. De Jonckheere, Lies Durnez, Patrick Suykerbuyk, Herwig Leirs, Françoise Portaels
Applied and Environmental Microbiology.2008; 74(21): 6547. CrossRef
Relationship between mycobacteria and amoebae: ecological and epidemiological concerns
V. Thomas, G. McDonnell
Letters in Applied Microbiology.2007; 45(4): 349. CrossRef

10,874 View
97 Download
Crossref

Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients: Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2007;45(1):1-9.
Published online March 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.1.1

Abstract
PDF

The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16S rDNA sequence analysis. Double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of Acanthamoeba isolates. The endosymbionts of KA/E9, KA/E22, and KA/E23 were surrounded by electron-translucent areas. No lacunae-like structures were observed in the endosymbionts of KA/E21, the bacterial cell walls of which were studded with host ribosomes. Comparative analyses of the 16S rDNA sequences showed that the endosymbionts of KA/E9, KA/E22 and KA/E23 were closely related to Caedibacter caryophilus, whereas the KA/E21 endosymbiont was assigned to the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum. In the 4 strains of Acanthamoeba, the hosts of the endosymbionts were identified as belonging to the Acanthamoeba castellanii complex, which corresponds to the T4 genotype. Acanthamoeba KA/E21 evidenced characteristics almost identical to those of KA/E6, with the exception of the existence of endosymbionts. The discovery of these endosymbionts from Acanthamoeba may prove essential to future studies focusing on interactions between the endosymbionts and the amoebic hosts.

Citations

Citations to this article as recorded by

Proteases of Acanthamoeba
Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani
Parasitology Research.2024;[Epub] CrossRef
Presence and diversity of free-living amoebae and their potential application as water quality indicators
Areum Choi, Ji Won Seong, Jeong Hyun Kim, Jun Young Lee, Hyun Jae Cho, Shin Ae Kang, Mi Kyung Park, Mi Jin Jeong, Seo Yeong Choi, Yu Jin Jeong, Hak Sun Yu
Parasites, Hosts and Diseases.2024; 62(2): 180. CrossRef
Contamination of fresh vegetables in municipal stores with pathogenic Acanthamoeba genotypes; a public health concern
Marziye Fatemi, Maryam Niyyati, Soheila Rouhani, Seyed Ahmad Karamati, Hamed Mirjalali, Panagiotis Karanis
International Journal of Environmental Health Research.2023; 33(10): 1010. CrossRef
The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiontAmoebophilus asiaticus
E Selberherr, T Penz, L König, B Conrady, A Siegl, M Horn, S Schmitz-Esser
FEMS Microbiology Ecology.2022;[Epub] CrossRef
A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
Pathogens.2021; 10(2): 225. CrossRef
Molecular characterization of bacterial, viral and fungal endosymbionts of Acanthamoeba isolates in keratitis patients of Iran
Elham Hajialilo, Mostafa Rezaeian, Maryam Niyyati, Mohammad Reza Pourmand, Mehdi Mohebali, Mehdi Norouzi, Kobra Razavi Pashabeyg, Sassan Rezaie, Sadegh Khodavaisy
Experimental Parasitology.2019; 200: 48. CrossRef
Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
Daniele Corsaro, Danielle Venditti
European Journal of Protistology.2018; 66: 26. CrossRef
Molecular identification of bacterial endosymbionts of Sappinia strains
Daniele Corsaro, Claudia Wylezich, Julia Walochnik, Danielle Venditti, Rolf Michel
Parasitology Research.2017; 116(2): 549. CrossRef
Identification of Paenibacillus as a Symbiont in Acanthamoeba
Vinicius José Maschio, Gertrudes Corção, Francielle Bücker, Karin Caumo, Marilise Brittes Rott
Current Microbiology.2015; 71(3): 415. CrossRef
Identifying endosymbiont bacteria associated with free‐living amoebae
Pilar Goñi, María Teresa Fernández, Encarnación Rubio
Environmental Microbiology.2014; 16(2): 339. CrossRef
Acanthamoeba castellanii cysts: new ultrastructural findings
Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo
Parasitology Research.2013; 112(3): 1125. CrossRef
Functional expression and characterization of an iron-containing superoxide dismutase of Acanthamoeba castellanii
Jung-Yeon Kim, Byoung-Kuk Na, Kyoung-Ju Song, Mi-Hyun Park, Yun-Kyu Park, Tong-Soo Kim
Parasitology Research.2012; 111(4): 1673. CrossRef
A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
Stephan Schmitz-Esser, Thomas Penz, Anja Spang, Matthias Horn
BMC Evolutionary Biology.2011;[Epub] CrossRef
Is Acanthamoeba pathogenicity associated with intracellular bacteria?
Graeme Neil Paterson, Michael Rittig, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Experimental Parasitology.2011; 129(2): 207. CrossRef
Detection of Bacterial Endosymbionts in Clinical Acanthamoeba Isolates
Alfonso Iovieno, Dolena R. Ledee, Darlene Miller, Eduardo C. Alfonso
Ophthalmology.2010; 117(3): 445. CrossRef
Bilateral Acanthamoeba Keratitis After Orthokeratology
Eun Chul Kim, Man Soo Kim
Cornea.2010; 29(6): 680. CrossRef
Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality
Vincent Thomas, Gerald McDonnell, Stephen P. Denyer, Jean-Yves Maillard
FEMS Microbiology Reviews.2010; 34(3): 231. CrossRef
Bilateral Acanthamoeba Keratitis After Orthokeratology
Eun Chul Kim, Man Soo Kim
Cornea.2009; 28(3): 348. CrossRef
Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef
Diversity of Bacterial Endosymbionts of Environmental Acanthamoeba Isolates
Stephan Schmitz-Esser, Elena R. Toenshoff, Susanne Haider, Eva Heinz, Verena M. Hoenninger, Michael Wagner, Matthias Horn
Applied and Environmental Microbiology.2008; 74(18): 5822. CrossRef

10,049 View
94 Download
Crossref

Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba: Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong; Korean J Parasitol 2006;44(4):331-341.
Published online December 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.4.331

Abstract
PDF

Actin binding proteins play key roles in cell structure and movement particularly as regulators of the assembly, stability and localization of actin filaments in the cytoplasm. In the present study, a cDNA clone encoding an actin bundling protein named as AhABP was isolated from Acanthamoeba healyi, a causative agent of granulomatous amebic encephalitis. This clone exhibited high similarity with genes of Physarum polycephalum and Dictyostelium discoideum, which encode actin bundling proteins. Domain search analysis revealed the presence of essential conserved regions, i.e., an active actin binding site and 2 putative calcium binding EF-hands. Transfected amoeba cells demonstrated that AhABP is primarily localized in phagocytic cups, peripheral edges, pseudopods, and in cortical cytoplasm where actins are most abundant. Moreover, AhABP after the deletion of essential regions formed ellipsoidal inclusions within transfected cells. High-speed co-sedimentation assays revealed that AhABP directly interacted with actin in the presence of up to 10 ?M of calcium. Under the electron microscope, thick parallel bundles were formed by full length AhABP, in contrast to the thin actin bundles formed by constructs with deletion sites. In the light of these results, we conclude that AhABP is a novel actin bundling protein that is importantly associated with actin filaments in the cytoplasm.

Citations

Citations to this article as recorded by

GILT in tumor cells improves T cell-mediated anti-tumor immune surveillance
Hongshuai Li, Yuan Wang, Mengchu Ma, Lihong Hu, Xinxin Zhang, Lingbiao Xin, Wei Zhang, Xiaoming Sun, Yuanyuan Ren, Xinting Wang, Jie Yang
Immunology Letters.2021; 234: 1. CrossRef
Acanthamoeba castellanii cysts: new ultrastructural findings
Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo
Parasitology Research.2013; 112(3): 1125. CrossRef
In Vitro Efficacies of Clinically Available Drugs against Growth and Viability of an Acanthamoeba castellanii Keratitis Isolate Belonging to the T4 Genotype
Abdul Mannan Baig, Junaid Iqbal, Naveed Ahmed Khan
Antimicrobial Agents and Chemotherapy.2013; 57(8): 3561. CrossRef

9,692 View
57 Download
Crossref

Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence: Won-Tae Kim, Hyun-Hee Kong, Young-Ran Ha, Yeon-Chul Hong, Hae Jin Jeong, Hak Sun Yu, Dong-Il Chung; Korean J Parasitol 2006;44(4):321-330.
Published online December 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.4.321

Abstract
PDF

The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and AK. In the present study, we have compared specific activity and cytopathic effects (CPE) of purified 33 kDa serine proteinases from Acanthamoeba strains with different degree of virulence (A. healyi OC-3A, A. lugdunensis KA/E2, and A. castellanii Neff). Trophozoites of the 3 strains revealed different degrees of CPE on human corneal epithelial (HCE) cells. The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor. This result indicated that PMSF-susceptible proteinase is the main component causing cytopathy to HCE cells by Acanthamoeba. The purified 33 kDa serine proteinase showed strong activity toward HCE cells and extracellular matrix proteins. The purified proteinase from OC-3A, the most virulent strain, demonstrated the highest enzyme activity compared to KA/E2, an ocular isolate, and Neff, a soil isolate. Polyclonal antibodies against the purified 33 kDa serine proteinase inhibit almost completely the proteolytic activity of culture supernatant of Acanthamoeba. In line with these results, the 33 kDa serine proteinase is suggested to play an important role in pathogenesis and to be the main component of virulence factor of Acanthamoeba.

Citations

Citations to this article as recorded by

Calcium ions in tap water may increase the adhesion ability of Acanthamoeba, potentially enhancing its cytopathic effects on corneal cells
Yu-Jen Wang, Yao-Tsung Chang, Tsun-Hsien Hsiao, Chun-Hsien Chen, Chih-Ming Tsai, Jian-Ming Huang
Parasite.2025; 32: 71. CrossRef
Proteases of Acanthamoeba
Behroz Mahdavi Poor, Jalil Rashedi, Vahid Asgharzadeh, Amirali Mirmazhary, Nazila Gheitarani
Parasitology Research.2024;[Epub] CrossRef
Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis
Alvie Loufouma-Mbouaka, Tania Martín-Pérez, Martina Köhsler, Zeynep Danisman, Maya Schwarz, Rounik Mazumdar, Ascel Samba-Louaka, Julia Walochnik
Parasites & Vectors.2024;[Epub] CrossRef
Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells
Abdullah Alhazmi, Laura E. Sidney, Andy Hopkinson, Hany M. Elsheikha
Acta Tropica.2024; 257: 107288. CrossRef
Assessment of in vitro dynamics of pathogenic environmental Acanthamoeba T4 and T9 genotypes isolated from three recreational lakes in Klang Valley, Malaysia over the HaCaT cell monolayer
Rohaya Abdul Halim, Hasseri Halim, Rosnani Hanim Mohd Hussain, Shafiq Aazmi, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
Journal of Water and Health.2024; 22(12): 2289. CrossRef
Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram
Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver
Processes.2023; 11(9): 2620. CrossRef
Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes
Daniele Corsaro
Microorganisms.2022; 10(11): 2162. CrossRef
In Vitro Cytopathogenic Activities of Acanthamoeba T3 and T4 Genotypes on HeLa Cell Monolayer
Rosnani Hanim Mohd Hussain, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Shafiq Aazmi, Hasseri Halim, Tengku Shahrul Anuar
Pathogens.2022; 11(12): 1474. CrossRef
Differential expression of Acanthamoeba castellanii proteins during amoebic keratitis in rats
Ana Carolina Carvalho-Silva, Camila H. Coelho, Cecília Cirelli, Frederico Crepaldi, Isabela Aurora Rodrigues-Chagas, Cinthia Furst, Daniel Carvalho Pimenta, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa
Experimental Parasitology.2021; 221: 108060. CrossRef
Peganum harmala Extract Has Antiamoebic Activity to Acanthamoeba triangularis Trophozoites and Changes Expression of Autophagy-Related Genes
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Chr
Pathogens.2021; 10(7): 842. CrossRef
Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells
Cecília Cirelli, Elaine Isabela Soares Mesquita, Isabela Aurora Rodrigues Chagas, Cinthia Furst, Cynara Oliveira Possamai, Jonatas Santos Abrahão, Ludmila Karen dos Santos Silva, Marina Felipe Grossi, Carlos Alberto Tagliati, Adriana Oliveira Costa
Parasitology Research.2020; 119(2): 659. CrossRef
Busting biofilms: free-living amoebae disrupt preformed methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium bovis biofilms
Kevin H. Martin, Grace I. Borlee, William H. Wheat, Mary Jackson, Bradley R. Borlee
Microbiology .2020; 166(8): 695. CrossRef
Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba
Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan
Acta Tropica.2020; 211: 105618. CrossRef
Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng
Parasites & Vectors.2020;[Epub] CrossRef
Extracellular vesicles and vesicle-free secretome of the protozoa Acanthamoeba castellanii under homeostasis and nutritional stress and their damaging potential to host cells
Diego de Souza Gonçalves, Marina da Silva Ferreira, Susie Coutinho Liedke, Kamilla Xavier Gomes, Gabriel Afonso de Oliveira, Pedro Ernesto Lopes Leão, Gabriele Vargas Cesar, Sergio H. Seabra, Juliana Reis Cortines, Arturo Casadevall, Leonardo Nimrichter,
Virulence.2018; 9(1): 818. CrossRef
Characterization of extracellular proteases of Acanthamoeba genotype T4 isolated from different sources in Iran
Behroz Mahdavi Poor, Abdolhossein Dalimi, Fatemeh Ghafarifar, Fariba Khoshzaban, Jalal Abdolalizadeh
Parasitology Research.2017; 116(12): 3373. CrossRef
Characterisation and expression analysis of trophozoite and cyst proteins of Acanthamoeba spp. isolated from Acanthamoeba keratitis (AK) patient
Himansu Sekhar Behera, Gita Satpathy
Molecular and Biochemical Parasitology.2016; 205(1-2): 29. CrossRef
Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition
Gaurav Kumar Pal, Suresh PV
RSC Advances.2016; 6(40): 33763. CrossRef
Acanthamoeba culbertsoni: Electron‐Dense Granules in a Highly Virulent Clinical Isolate
Bibiana Chávez‐Munguía, Lizbeth Salazar‐Villatoro, Maritza Omaña‐Molina, Martha Espinosa‐Cantellano, Elizabeth Ramírez‐Flores, Jacob Lorenzo‐Morales, Adolfo Martínez‐Palomo
Journal of Eukaryotic Microbiology.2016; 63(6): 744. CrossRef
Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases
Yu-Zhong Zhang, Li-Yuan Ran, Chun-Yang Li, Xiu-Lan Chen, F. E. Löffler
Applied and Environmental Microbiology.2015; 81(18): 6098. CrossRef
Acanthamoeba Protease Activity Promotes Allergic Airway Inflammation via Protease-Activated Receptor 2
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Dong-Hee Kim, Hak Sun Yu, Venuprasad K. Poojary
PLoS ONE.2014; 9(3): e92726. CrossRef
Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism
Li-Yuan Ran, Hai-Nan Su, Ming-Yang Zhou, Lei Wang, Xiu-Lan Chen, Bin-Bin Xie, Xiao-Yan Song, Mei Shi, Qi-Long Qin, Xiuhua Pang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang
Journal of Biological Chemistry.2014; 289(9): 6041. CrossRef
Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family
Li‐Yuan Ran, Hai‐Nan Su, Guo‐Yan Zhao, Xiang Gao, Ming‐Yang Zhou, Peng Wang, Hui‐Lin Zhao, Bin‐Bin Xie, Xi‐Ying Zhang, Xiu‐Lan Chen, Bai‐Cheng Zhou, Yu‐Zhong Zhang
Molecular Microbiology.2013; 90(5): 997. CrossRef
Pathological characteristics of the different stages of Acanthamoeba keratitis
Yuzhao Sun, Jing Hong, Pei Zhang, Rongmei Peng, Gege Xiao
Histopathology.2013; 63(6): 862. CrossRef
Proteases fromEntamoebaspp. and Pathogenic Free-Living Amoebae as Virulence Factors
Jesús Serrano-Luna, Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortiz-Estrada, Mireya de la Garza
Journal of Tropical Medicine.2013; 2013: 1. CrossRef
Acanthamoeba interactions with the blood–brain barrier under dynamic fluid flow
James Edwards-Smallbone, Richard J. Pleass, Naveed A. Khan, Robin J. Flynn
Experimental Parasitology.2012; 132(3): 367. CrossRef
Host-Parasite Interaction: Parasite-Derived and -Induced Proteases That Degrade Human Extracellular Matrix
Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortíz-Estrada, Mireya de la Garza, Jesús Serrano-Luna
Journal of Parasitology Research.2012; 2012: 1. CrossRef
Serine protease activities in Leishmania (Leishmania) chagasi promastigotes
Raquel Elisa da Silva-López, Tatiana Resende dos Santos, José Andrés Morgado-Díaz, Marcelo Neves Tanaka, Salvatore Giovanni de Simone
Parasitology Research.2010; 107(5): 1151. CrossRef
Pathogenesis of Acanthamoeba Keratitis
Noorjahan Panjwani
The Ocular Surface.2010; 8(2): 70. CrossRef
Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity
Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral
Infection and Immunity.2010; 78(9): 4001. CrossRef
Differential effects of α-helical and β-hairpin antimicrobial peptides against Acanthamoeba castellanii
R. S. SACRAMENTO, R. M. MARTINS, A. MIRANDA, A. S. S. DOBROFF, S. DAFFRE, A. S. FORONDA, D. DE FREITAS, S. SCHENKMAN
Parasitology.2009; 136(8): 813. CrossRef
Elastase secretion in Acanthamoeba polyphaga
Gabriela A. Ferreira, Ana C.M. Magliano, Elizabeth M.F. Pral, Silvia C. Alfieri
Acta Tropica.2009; 112(2): 156. CrossRef
Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong
Eukaryotic Cell.2008; 7(9): 1513. CrossRef
Differentially expressed genes of Acanthamoeba castellanii during encystation
Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong
The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef

11,045 View
88 Download
Crossref

Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear: Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu; Korean J Parasitol 2006;44(4):313-320.
Published online December 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.4.313

Abstract
PDF

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.

Citations

Citations to this article as recorded by

Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation
Da-In Lee, Sung Hee Park, Shin-Ae Kang, Do Hyun Kim, Sun Hyun Kim, So Yeon Song, Sang Eun Lee, Hak Sun Yu
The Korean Journal of Parasitology.2022; 60(4): 229. CrossRef
Orthokeratology lens-related Acanthamoeba keratitis: case report and analytical review
Jinfang Wu, Huatao Xie
Journal of International Medical Research.2021;[Epub] CrossRef
Infectious keratitis and orthokeratology lens use: a systematic review
Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L. Young
Infection.2017; 45(6): 727. CrossRef
The Role of Ultraviolet Radiation in the Ocular System of Mammals
Mercede Majdi, Behrad Milani, Asadolah Movahedan, Lisa Wasielewski, Ali Djalilian
Photonics.2014; 1(4): 347. CrossRef
Orthokeratology lens related infections
Kelvin Ho-Nam Wan
World Journal of Ophthalmology.2014; 4(3): 63. CrossRef
Riboflavin and Ultraviolet Light A Therapy as an Adjuvant Treatment for Medically Refractive Acanthamoeba Keratitis
Yasin A. Khan, Renata T. Kashiwabuchi, Suy Anne Martins, Juan M. Castro-Combs, Sachin Kalyani, Philip Stanley, David Flikier, Ashley Behrens
Ophthalmology.2011; 118(2): 324. CrossRef
Bilateral Acanthamoeba Keratitis After Orthokeratology
Eun Chul Kim, Man Soo Kim
Cornea.2010; 29(6): 680. CrossRef
Twenty Years of Acanthamoeba Keratitis
F R S Carvalho, A S Foronda, M J Mannis, A L Höfling-Lima, R Belfort, Denise de Freitas
Cornea.2009; 28(5): 516. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
Bilateral Acanthamoeba Keratitis After Orthokeratology
Eun Chul Kim, Man Soo Kim
Cornea.2009; 28(3): 348. CrossRef
Trends in Microbial Keratitis Associated With Orthokeratology
Kathleen G. Watt, Helen A. Swarbrick
Eye & Contact Lens: Science & Clinical Practice.2007; 33(6): 373. CrossRef

14,053 View
110 Download
Crossref

Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani: Joon-Hyuck Choi, Jae-Hyuk Lee, Hak-Sun Yu, Hae-Jin Jeong, Jin Kim, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2006;44(3):187-196.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.187

Abstract
PDF

The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and reproduction of P. westermani. In this study, a cDNA clone encoding a 47 kDa hemoglobinase of P. westermani was characterized by sequencing analysis, and its localization was investigated immunohistochemically. The phylogenetic tree prepared based on the hemoglobinase gene showed high homology with hemoglobinases of Fasciola hepatica and Schistosoma spp. Moreover, recombinant P. westermani hemoglobinase degradaded human hemoglobin at acidic pH (from 3.0 to 5.5) and its activity was almost completely inhibited by E-64, a cysteine proteinase inhibitor. Immunohistochemical studies showed that P. westermani hemoglobinase was localized in the epithelium of the adult worm intestine implying that the protein has a specific function. These observations suggest that hemoglobinase may act as a digestive enzyme for acquisition of nutrients from host hemoglobin. Further investigations may provide insights into hemoglobin catabolism in P. westermani.

Citations

Citations to this article as recorded by

Multi-stage transcriptome profiling of the neglected food-borne echinostome Artyfechinostomum sufrartyfex reveal potential diagnostic and drug targets
Suman Dahal, Pratibha Gour, Saurabh Raghuvanshi, Yugal Kishore Prasad, Dipshikha Saikia, Sudeep Ghatani
Acta Tropica.2022; 233: 106564. CrossRef
Characterization and localization of antigens for serodiagnosis of human paragonimiasis
Kurt C. Curtis, Kerstin Fischer, Young-Jun Choi, Makedonka Mitreva, Gary J. Weil, Peter U. Fischer
Parasitology Research.2021; 120(2): 535. CrossRef
Cysteine proteases as digestive enzymes in parasitic helminths
Conor R. Caffrey, Louise Goupil, Karina M. Rebello, John P. Dalton, David Smith, Aaron R. Jex
PLOS Neglected Tropical Diseases.2018; 12(8): e0005840. CrossRef
Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl
Experimental Parasitology.2013; 135(2): 297. CrossRef
Paragonimus worm from a New Guinea native in 1926
Wenlin Wang, David Blair, Tian Min, Fang Li, Dianhua Wang
Asian Pacific Journal of Tropical Medicine.2011; 4(1): 76. CrossRef
Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis
Thewarach Laha, Jittiyawadee Sripa, Banchob Sripa, Mark Pearson, Leon Tribolet, Sasithorn Kaewkes, Paiboon Sithithaworn, Paul J. Brindley, Alex Loukas
International Journal of Infectious Diseases.2008; 12(6): e49. CrossRef

9,709 View
79 Download
Crossref

Genetic diversity of Acanthamoeba isolates from ocean sediments: Hua Liu, Young-Ran Ha, Sung-Tae Lee, Yean-Chul Hong, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2006;44(2):117-125.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.117

Abstract
PDF

Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial cells versus reference strains. All isolates belonged to morphologic group II. Total of 16 restriction phenotypes of mtDNA from 18 isolates demonstrated the genetic diversity of Acanthamoeba in ocean sediments. Phylogenetic analysis using 18s rDNA sequences revealed that the 18 isolates were distinct from morphological groups I and III. Fifteen isolates showed close relatedness with 17 clinical isolates and A. castellanii Castellani and formed a lineage equivalent to T4 genotype of Byers' group. Two reference strains from ocean sediment, A. hatchetti BH-2 and A. griffini S-7 clustered unequivocally with these 15 isolates. Diversity among isolates was also evident from their cytopathic effects on human corneal cells. This is the first time describing Acanthamoeba diversity in ocean sediments in Korea.

Citations

Citations to this article as recorded by

Learning from the rDNA Operon: A Reanalysis of the Acanthamoeba palestinensis Group
Daniele Corsaro
Microorganisms.2024; 12(10): 2105. CrossRef
The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Paul A. Fuerst
Microorganisms.2023; 11(2): 295. CrossRef
Detection of potentially pathogenic free-living amoebae from the Caspian Sea and hospital ward dust of teaching hospitals in Guilan, Iran
Mohammad Reza Mahmoudi, Nozhat Zebardast, Frederick R. Masangkay, Panagiotis Karanis
Journal of Water and Health.2021; 19(2): 278. CrossRef
Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
Stas Malavin, Lyubov Shmakova
European Journal of Protistology.2020; 73: 125671. CrossRef
Isolation and identification of free-living amoeba from the hot springs and beaches of the Caspian Sea
Alireza Latifi, Mahboobeh Salami, Elham Kazemirad, Mohammad Soleimani
Parasite Epidemiology and Control.2020; 10: e00151. CrossRef
Update on Acanthamoeba phylogeny
Daniele Corsaro
Parasitology Research.2020; 119(10): 3327. CrossRef
Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
Daniele Corsaro, Danielle Venditti
European Journal of Protistology.2018; 66: 26. CrossRef
Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater
Penelope H. Dobrowsky, Sehaam Khan, Thomas E. Cloete, Wesaal Khan
Parasites & Vectors.2016;[Epub] CrossRef
Metagenomic characterization of viral communities in Goseong Bay, Korea
Jinik Hwang, So Yun Park, Mirye Park, Sukchan Lee, Yeonhwa Jo, Won Kyong Cho, Taek-Kyun Lee
Ocean Science Journal.2016; 51(4): 599. CrossRef
Pandoraviruses: Amoeba Viruses with Genomes Up to 2.5 Mb Reaching That of Parasitic Eukaryotes
Nadège Philippe, Matthieu Legendre, Gabriel Doutre, Yohann Couté, Olivier Poirot, Magali Lescot, Defne Arslan, Virginie Seltzer, Lionel Bertaux, Christophe Bruley, Jérome Garin, Jean-Michel Claverie, Chantal Abergel
Science.2013; 341(6143): 281. CrossRef
Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga
Otmane Lamrabet, Michel Drancourt
Applied and Environmental Microbiology.2013; 79(5): 1606. CrossRef
Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida)
Daniele Corsaro, Danielle Venditti
Parasitology Research.2010; 107(1): 233. CrossRef
Barcoding Amoebae: Comparison of SSU, ITS and COI Genes as Tools for Molecular Identification of Naked Lobose Amoebae
Elena Nassonova, Alexey Smirnov, Jose Fahrni, Jan Pawlowski
Protist.2010; 161(1): 102. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
Biodiversity of amoebae and amoebae‐resisting bacteria in a drinking water treatment plant
Vincent Thomas, Jean‐François Loret, Michel Jousset, Gilbert Greub
Environmental Microbiology.2008; 10(10): 2728. CrossRef
Relationship between mycobacteria and amoebae: ecological and epidemiological concerns
V. Thomas, G. McDonnell
Letters in Applied Microbiology.2007; 45(4): 349. CrossRef

9,589 View
70 Download
Crossref

Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses: Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2005;43(1):7-13.
Published online March 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.1.7

Abstract
PDF

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.

Citations

Citations to this article as recorded by

The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Paul A. Fuerst
Microorganisms.2023; 11(2): 295. CrossRef
On the diversity and clinical importance of Acanthamoeba spp. from Group 1
Daniele Corsaro
Parasitology Research.2021; 120(6): 2057. CrossRef
Isolates from ancient permafrost help to elucidate species boundaries in Acanthamoeba castellanii complex (Amoebozoa: Discosea)
Stas Malavin, Lyubov Shmakova
European Journal of Protistology.2020; 73: 125671. CrossRef
Update on Acanthamoeba phylogeny
Daniele Corsaro
Parasitology Research.2020; 119(10): 3327. CrossRef
Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef
Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia
Li-Li Chan, Joon-Wah Mak, Yoon-Tong Low, Thuan-Tzen Koh, Init Ithoi, Shar Mariam Mohamed
Acta Tropica.2011; 117(1): 23. CrossRef
Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef
Acanthamoeba keratitis due to Acanthamoeba genotype T4 in a non-contact-lens wearer in Turkey
Hatice Ertabaklar, Meral Türk, Volkan Dayanir, Sema Ertuğ, Julia Walochnik
Parasitology Research.2007; 100(2): 241. CrossRef

8,760 View
78 Download
Crossref

Brief Communication

Infection status of intestinal parasites in children living in residential institutions in Metro Manila, the Philippines: Eleonor T Baldo, Vicente Y Belizario, Winifreda U De Leon, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2004;42(2):67-70.
Published online June 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.2.67

Abstract
PDF

A small scale survey was performed to know the infection status of intestinal parasite in children of the residential institutions and street communities in Metro Manila, Philippines. A total of 284 stool samples from 11 institutions and 3 street communities was examined by the formalin-ether concentration method. The scotch tape anal swab was adapted to 121 children to investigate the infection status of Enterovius vermicularis. It was found out that 62.0% of the children examined were positive for one or more intestinal parasites. Multiple infections were observed in 34.2% of the children. Among 172 children who gave detail information, the prevalence for Ascaris lumbricoides, Trichuris trichiura, and hookworm was 36.0%, 44.8%, and 7.0% respectively. Of the children examined, 47.7% were found to be harboring parasitic protozoans such as Entamoeba histolytica, Giardia lamblia, and Blastocystis hominis. The most prevalent of these protozoans was B. hominis with an infection rate of 40.7%. The prevalence of these infections among children living in institutions was relatively high. More efforts should be made to implement anthelminthic programs including bi-annual follow-up treatments.

Citations

Citations to this article as recorded by

Prevalence of intestinal parasites in street dwellers attending a shelter in Cali, Colombia
Jorge Iván Zapata-Valencia, Diana Maritza Jurado-Orejuela, Ofelia Flórez-Echeverry, Erica Marcela Aristizábal-Giraldo, Jhonathan León Gallego-Franco, María Camila Yolanda Ramírez-Uribe, Jemina Rentería-Molina, Alejandra Sandoval-Villareal, Yermaín Ulabarr
Biomédica.2024; 44(4): 524. CrossRef
Epidemiology of Blastocystis Infection: A Review of Data from Poland in Relation to Other Reports
Monika Rudzińska, Katarzyna Sikorska
Pathogens.2023; 12(8): 1050. CrossRef
Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia
Majed H Wakid, Waad T Aldahhasi, Muslimah N Alsulami, Asmaa M. El-Kady, Hatem Elshabrawy
Infection and Drug Resistance.2022; Volume 15: 491. CrossRef
Development of a salivary IgA detection method for accurate diagnosis of amebiasis
Davin Edric V. Adao, Angeline Odelia C. Li, Alexander Edward S. Dy, Windell L. Rivera
Journal of Parasitic Diseases.2022; 46(3): 714. CrossRef
Comparative molecular prevalence and subtypes distribution of Blastocystis sp. a potentially zoonotic infection isolated from symptomatic and asymptomatic patients in Iran: A systematic review and meta-analysis
Ali Asghari, Soheil Hassanipour, Gholamreza Hatam
Acta Parasitologica.2021; 66(3): 745. CrossRef
The control of soil-transmitted helminthiases in the Philippines: the story continues
Mary Lorraine S. Mationg, Veronica L. Tallo, Gail M. Williams, Catherine A. Gordon, Archie C. A. Clements, Donald P. McManus, Darren J. Gray
Infectious Diseases of Poverty.2021;[Epub] CrossRef
Epidemiology and subtype distribution of Blastocystis in humans: A review
Supaluk Popruk, Davin Edric V. Adao, Windell L. Rivera
Infection, Genetics and Evolution.2021; 95: 105085. CrossRef
Frequency of Intestinal Parasitic Infection Among Working Children in Tehran, Iran
Borna Salemi, Abdoulreza Esteghamati, Shirin Sayyahfar, Farah Bokharaei-Salim, Hossein Keyvani, Khadijeh Khanaliha
Archives of Pediatric Infectious Diseases.2019;[Epub] CrossRef
Prevalence of intestinal parasitic infections and associated factors among street children in Jimma town; south West Ethiopia in 2019: a cross sectional study
Sabit Zenu, Eshetu Alemayehu, Kifle Woldemichael
BMC Public Health.2019;[Epub] CrossRef
Co-parasitism of intestinal protozoa and Schistosoma japonicum in a rural community in the Philippines
Kosala Gayan Weerakoon, Catherine A. Gordon, Gail M. Williams, Pengfei Cai, Geoffrey N. Gobert, Remigio M. Olveda, Allen G. Ross, David U. Olveda, Donald P. McManus
Infectious Diseases of Poverty.2018;[Epub] CrossRef
Intestinal parasitic infections in Iranian preschool and school children: A systematic review and meta-analysis
Ahmad Daryani, Saeed Hosseini-Teshnizi, Seyed-Abdollah Hosseini, Ehsan Ahmadpour, Shahabeddin Sarvi, Afsaneh Amouei, Azadeh Mizani, Sara Gholami, Mehdi Sharif
Acta Tropica.2017; 169: 69. CrossRef
Subtyping of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Makkah, Saudi Arabia
Raafat T. Mohamed, Mohammed A. El-Bali, Anhar A. Mohamed, Mona A. Abdel-Fatah, Mohamed A. EL-Malky, Nawras M. Mowafy, Dina A. Zaghlool, Rowaida A. Bakri, Saeed A. Al-Harthi
Parasites & Vectors.2017;[Epub] CrossRef
Prevalence and risk factors of Blastocystis infection among underprivileged communities in rural Malaysia
Nabilah Amelia Mohammad, Hesham M. Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar
Asian Pacific Journal of Tropical Medicine.2017; 10(5): 491. CrossRef
Health and water quality benefits of alternative sewerage systems in Metro Manila, Philippines
Rosalina Palanca-Tan
Environment and Urbanization.2017; 29(2): 567. CrossRef
Intestinal Parasitic Infection and Body Mass Index among School Children in Oshodi Lagos Nigeria
Ajayi MB
Advances in Cytology & Pathology.2017;[Epub] CrossRef
Subtype analysis of Blastocystis sp. isolates from human and canine hosts in an urban community in the Philippines
Maria Luz B. Belleza, John Carlo B. Reyes, Pilarita N. Tongol-Rivera, Windell L. Rivera
Parasitology International.2016; 65(3): 291. CrossRef
Prevalence of intestinal parasites among street beggars in Jimma town, Southwest Ethiopia
Ashebir Lakew, Gebre Kibru, Abdissa Biruksew
Asian Pacific Journal of Tropical Disease.2015; 5: S85. CrossRef
Tongkat Ali (Eurycoma longifolia): a possible therapeutic candidate against Blastocystis sp.
Sonal Girish, Suresh Kumar, Norhaniza Aminudin
Parasites & Vectors.2015;[Epub] CrossRef
The distribution of Blastocystis subtypes in isolates from Qatar
Marawan Abu-Madi, Mahmoud Aly, Jerzy M. Behnke, C. Graham Clark, Hanan Balkhy
Parasites & Vectors.2015;[Epub] CrossRef
Parasitosis intestinal y factores de riesgo en niños de los asentamientos subnormales, Florencia-Caquetá, Colombia
Tarín A. Lucero-Garzón, Luis A. Álvarez-Motta, Jeison F. Chicue-López, Deyirley López-Zapata, Cristian A. Mendoza-Bergaño
Revista Facultad Nacional de Salud Pública.2015; 33(2): 171. CrossRef
The pathogenic role of Blastocystis isolated from patients with irritable bowel syndrome and colitis from Iasi, Romania
Doina S. Matiut, Lucian Hritcu
Acta Parasitologica.2014;[Epub] CrossRef
The Health Status of Street Children and Youth in Low- and Middle-Income Countries: A Systematic Review of the Literature
Jessica Woan, Jessica Lin, Colette Auerswald
Journal of Adolescent Health.2013; 53(3): 314. CrossRef
Prevalence, predictors and clinical significance of Blastocystis sp. in Sebha, Libya
Awatif M Abdulsalam, Init Ithoi, Hesham M Al-Mekhlafi, Abdul Hafeez Khan, Abdulhamid Ahmed, Johari Surin, Joon Wah Mak
Parasites & Vectors.2013;[Epub] CrossRef
Blastocystis infection in Malaysia: Evidence of waterborne and human-to-human transmissions among the Proto-Malay, Negrito and Senoi tribes of Orang Asli
Tengku Shahrul Anuar, Mohamed Kamel Abdul Ghani, Siti Nor Azreen, Fatmah Md Salleh, Norhayati Moktar
Parasites & Vectors.2013;[Epub] CrossRef
Association of dental caries and weight status in 6- to 7-year-old Filipino children
Roswitha Heinrich-Weltzien, Bella Monse, Habib Benzian, Joachim Heinrich, Katrin Kromeyer-Hauschild
Clinical Oral Investigations.2013; 17(6): 1515. CrossRef
Factors associated with parasitic infection amongst street children in orphanages across Lima, Peru
Chris Bailey, Sonia Lopez, Anahí Camero, Carmen Taiquiri, Yanina Arhuay, David A J Moore
Pathogens and Global Health.2013; 107(2): 52. CrossRef
Drinking water is a significant predictor ofBlastocystisinfection among rural Malaysian primary schoolchildren
AWATIF M. ABDULSALAM, INIT ITHOI, HESHAM M. AL-MEKHLAFI, ABDULHAMID AHMED, JOHARI SURIN, JOON-WAH MAK
Parasitology.2012; 139(8): 1014. CrossRef
Untreated severe dental decay: a neglected determinant of low Body Mass Index in 12-year-old Filipino children
Habib Benzian, Bella Monse, Roswitha Heinrich-Weltzien, Martin Hobdell, Jan Mulder, Wim van Palenstein Helderman
BMC Public Health.2011;[Epub] CrossRef
Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a ‘gold standard’
M.R. Tarafder, H. Carabin, L. Joseph, E. Balolong, R. Olveda, S.T. McGarvey
International Journal for Parasitology.2010; 40(4): 399. CrossRef
Epidemiology and control of human gastrointestinal parasites in children
Michael O Harhay, John Horton, Piero L Olliaro
Expert Review of Anti-infective Therapy.2010; 8(2): 219. CrossRef
The effect of family influence on indicators associated with street life among Filipino street children
Ray M. Merrill, Levi Njord, Rebecca Njord, Curtis Read, Jeanette D.R. Pachano
Vulnerable Children and Youth Studies.2010; 5(2): 142. CrossRef
The prevalence of intestinal parasites in the province of Izmir, Turkey
Hande Dagci, Özgür Kurt, Mete Demirel, Ipek Östan, Naser Reza Azizi, Aliye Mandiracioglu, Cengiz Yurdagül, Mehmet Tanyüksel, Ece Eroglu, Mucide Ak
Parasitology Research.2008; 103(4): 839. CrossRef
Prevalence of Giardia lamblia with or without diarrhea in South East, South East Asia and the Far East
Hassan H. Dib, Si Qi Lu, Shao Fang Wen
Parasitology Research.2008; 103(2): 239. CrossRef
Large-Scale Preventive Chemotherapy for the Control of Helminth Infection in Western Pacific Countries: Six Years Later
Antonio Montresor, Dai Tran Cong, Mouth Sinuon, Reiko Tsuyuoka, Chitsavang Chanthavisouk, Hanne Strandgaard, Raman Velayudhan, Corinne M. Capuano, Tuan Le Anh, Ah S. Tee Dató, Simon Brooker
PLoS Neglected Tropical Diseases.2008; 2(8): e278. CrossRef
New Insights on Classification, Identification, and Clinical Relevance ofBlastocystisspp
Kevin S. W. Tan
Clinical Microbiology Reviews.2008; 21(4): 639. CrossRef

12,830 View
230 Download
Crossref

Original Article

Purification and characterization of a 33 kDa serine protease from Acanthamoeba lugdunensis KA/E2 isolated from a Korean keratitis patient: Hyo-Kyung Kim, Young-Ran Ha, Hak-Sun Yu, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2003;41(4):189-196.
Published online December 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.4.189

Abstract
PDF

In order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient. The ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on CM-Sepharose, Sephacryl S-200, and mono Q-anion exchange column. The purified 33 kDa protease had a pH optimum of 8.5 and a temperature optimum of 55℃. Phenylmethylsulfonylfluoride and 4-(2-Aminoethyl)-benzenesulfonyl-fluoride, both serine protease specific inhibitors, inhibited almost completely the activity of the 33 kDa protease whereas other classes of inhibitors did not affect its activity. The 33 kDa enzyme degraded various extracellular matrix proteins and serum proteins. Our results strongly suggest that the 33 kDa serine protease secreted from this keratopathogenic Acanthamoeba play important roles in the pathogenesis of amoebic keratitis, such as in corneal tissue invasion, immune evasion and nutrient uptake.

Citations

Citations to this article as recorded by

Identification of an Antimicrobial Protease from Acanthamoeba via a Novel Zymogram
Alvaro de Obeso Fernández del Valle, Luis Javier Melgoza-Ramírez, María Fernanda Esqueda Hernández, Alfonso David Rios-Pérez, Sutherland K. Maciver
Processes.2023; 11(9): 2620. CrossRef
The gene expression and proteomic profiling of Acanthamoeba isolates
Chayan Sharma, Sumeeta Khurana, Alka Bhatia, Amit Arora, Amit Gupta
Experimental Parasitology.2023; 255: 108630. CrossRef
Comparative Surfaceome Analysis of Clonal Histomonas meleagridis Strains with Different Pathogenicity Reveals Strain-Dependent Profiles
Marcelo de Jesus Ramires, Karin Hummel, Tamas Hatfaludi, Petra Riedl, Michael Hess, Ivana Bilic
Microorganisms.2022; 10(10): 1884. CrossRef
Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins
Abigail Betanzos, Cecilia Bañuelos, Esther Orozco
Genes.2019; 10(8): 618. CrossRef
Acanthamoeba spp. un agente oportunista en infecciones humanas
Martín Cabello-Vílchez
Revista de Investigación de la Universidad Privada Norbert Wiener.2019; 4(1): 11. CrossRef
Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
Showgy Y. Ma’ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G. Svärd, Armando Jardim
PLOS Neglected Tropical Diseases.2017; 11(12): e0006120. CrossRef
Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut
Goutam Banerjee, Sandip Mukherjee, Shelley Bhattacharya, Arun K. Ray
Arabian Journal for Science and Engineering.2016; 41(1): 9. CrossRef
Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition
Gaurav Kumar Pal, Suresh PV
RSC Advances.2016; 6(40): 33763. CrossRef
Diversity, Structures, and Collagen-Degrading Mechanisms of Bacterial Collagenolytic Proteases
Yu-Zhong Zhang, Li-Yuan Ran, Chun-Yang Li, Xiu-Lan Chen, F. E. Löffler
Applied and Environmental Microbiology.2015; 81(18): 6098. CrossRef
Microarray and KOG analysis of Acanthamoeba healyi genes up-regulated by mouse-brain passage
Eun-Kyung Moon, Ying-Hua Xuan, Hyun-Hee Kong
Experimental Parasitology.2014; 143: 69. CrossRef
Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism
Li-Yuan Ran, Hai-Nan Su, Ming-Yang Zhou, Lei Wang, Xiu-Lan Chen, Bin-Bin Xie, Xiao-Yan Song, Mei Shi, Qi-Long Qin, Xiuhua Pang, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang
Journal of Biological Chemistry.2014; 289(9): 6041. CrossRef
Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family
Li‐Yuan Ran, Hai‐Nan Su, Guo‐Yan Zhao, Xiang Gao, Ming‐Yang Zhou, Peng Wang, Hui‐Lin Zhao, Bin‐Bin Xie, Xi‐Ying Zhang, Xiu‐Lan Chen, Bai‐Cheng Zhou, Yu‐Zhong Zhang
Molecular Microbiology.2013; 90(5): 997. CrossRef
Proteases fromEntamoebaspp. and Pathogenic Free-Living Amoebae as Virulence Factors
Jesús Serrano-Luna, Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortiz-Estrada, Mireya de la Garza
Journal of Tropical Medicine.2013; 2013: 1. CrossRef
Host-Parasite Interaction: Parasite-Derived and -Induced Proteases That Degrade Human Extracellular Matrix
Carolina Piña-Vázquez, Magda Reyes-López, Guillermo Ortíz-Estrada, Mireya de la Garza, Jesús Serrano-Luna
Journal of Parasitology Research.2012; 2012: 1. CrossRef
Distribution of Proteolytic Activity in the Different Protein Fractions of Tropical Shrimp Head Waste
Raghu Ganugula, Rupsankar Chakrabarti, Krothapalli Raja Surya Sambasiva Rao
Food Biotechnology.2008; 22(1): 18. CrossRef
Protease activity in extracellular products secreted in vitro by trophozoites of Giardia duodenalis
Thaís Batista de Carvalho, Érica Boarato David, Silvana Torossian Coradi, Semíramis Guimarães
Parasitology Research.2008; 104(1): 185. CrossRef
Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence
Won-Tae Kim, Hyun-Hee Kong, Young-Ran Ha, Yeon-Chul Hong, Hae Jin Jeong, Hak Sun Yu, Dong-Il Chung
The Korean Journal of Parasitology.2006; 44(4): 321. CrossRef
Detection of a serine proteinase gene in Acanthamoeba genotype T6 (Amoebozoa: Lobosea)
Marion Blaschitz, Martina Köhsler, Horst Aspöck, Julia Walochnik
Experimental Parasitology.2006; 114(1): 26. CrossRef
Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi
E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong
Eukaryotic Cell.2006; 5(1): 125. CrossRef
Étude de l’effet des oligomères procyanidoliques sur la fibrillogénèse de la cornée
A.M. Robert, L. Robert, G. Renard
Journal Français d'Ophtalmologie.2005; 28(10): 1017. CrossRef
Expression of the nfa1 Gene Cloned from Pathogenic Naegleria fowleri in Nonpathogenic N. gruberi Enhances Cytotoxicity against CHO Target Cells In Vitro
Seok-Ryoul Jeong, Sang-Chul Lee, Kyoung-Ju Song, Sun Park, Kyongmin Kim, Myung-Hee Kwon, Kyung-il Im, Ho-Joon Shin
Infection and Immunity.2005; 73(7): 4098. CrossRef
Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood–brain barrier
Selwa Alsam, James Sissons, Samantha Jayasekera, Naveed Ahmed Khan
Journal of Infection.2005; 51(2): 150. CrossRef

8,525 View
87 Download
Crossref

Brief Communications

Molecular identification of Korean Trichinella isolates: Woon-Mok Sohn, Sun Huh, Dong-Il Chung, Edoardo Pozio; Korean J Parasitol 2003;41(2):125-127.
Published online June 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.2.125

Abstract
PDF

Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.

Citations

Citations to this article as recorded by

From wildlife to humans: The global distribution of Trichinella species and genotypes in wildlife and wildlife-associated human trichinellosis
Cody J. Malone, Antti Oksanen, Samson Mukaratirwa, Rajnish Sharma, Emily Jenkins
International Journal for Parasitology: Parasites and Wildlife.2024; 24: 100934. CrossRef
The dynamics of Trichinella spiralis epidemiology: Out to pasture?
K.D. Murrell
Veterinary Parasitology.2016; 231: 92. CrossRef
The induction of the collagen capsule synthesis by Trichinella spiralis is closely related to protease-activated receptor 2
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Bo Young Kim, Hak Sun Yu
Veterinary Parasitology.2016; 230: 56. CrossRef
Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice
Ki-Back Chu, Sang-Soo Kim, Su-Hwa Lee, Dong-Hun Lee, Ah-Ra Kim, Fu-Shi Quan
The Korean Journal of Parasitology.2016; 54(5): 637. CrossRef
Chemotherapeutic drugs for common parasitic diseases in Korea
Sun Huh
Journal of the Korean Medical Association.2013; 56(6): 513. CrossRef
An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam
Nguyen Van De, Nguyen Vu Trung, Nguyen Hong Ha, Vu Thi Nga, Nguyen Minh Ha, Pham Thanh Thuy, Le Van Duyet, Jong-Yil Chai
The Korean Journal of Parasitology.2012; 50(4): 339. CrossRef
Molecular characterization of 45kDa aspartic protease of Trichinella spiralis
Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
Veterinary Parasitology.2012; 190(3-4): 510. CrossRef
The Fifth Outbreak of Trichinosis in Korea
Ji-Young Rhee, Sung-Tae Hong, Hye-Jung Lee, Min Seo, Suk-Bae Kim
The Korean Journal of Parasitology.2011; 49(4): 405. CrossRef
The epidemiology of human trichinellosis in China during 2004–2009
J. Cui, Z.Q. Wang, B.L. Xu
Acta Tropica.2011; 118(1): 1. CrossRef
World distribution of Trichinella spp. infections in animals and humans
Edoardo Pozio
Veterinary Parasitology.2007; 149(1-2): 3. CrossRef
Characterisation of Trichinella isolates from Bulgaria by molecular typing and cross-breeding
R. Kurdova, N. Müller, N. Tsvetkova, L. Michov, D. Georgieva, M. Ivanova, B Gottstein
Veterinary Parasitology.2004; 123(3-4): 179. CrossRef

8,975 View
87 Download
Crossref

A human case of Echinostoma hortense (Trematoda: Echinostomatidae) infection diagnosed by gastroduodenal endoscopy in Korea: Chang-Min Cho, Won-Young Tak, Young-Oh Kweon, Sung-Kook Kim, Yong-Hwan Choi, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2003;41(2):117-120.
Published online June 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.2.117

Abstract
PDF

A human Echinostoma hortense infection was diagnosed by gastroduodenoscopy. An 81-year-old Korean male, living in Yeongcheon-shi, Gyeongsangbuk-do and with epigastric discomfort of several days duration, was subjected to upper gastrointestinal endoscopy. He was in the habit of eating fresh water fish. Two live worms were found in the duodenal bulb area and were removed using an endoscopic forcep. Based on their morphological characteristics, the worms were identified as E. hortense. The patient was treated with praziquantel 10 mg/kg as a single dose. The source of the infection in this case remains unclear, but the fresh water fish consumed, including the loach, may have been the source. This is the second case of E. hortense infection diagnosed by endoscopy in Korea.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Echinostoma hortense detected by endoscopy and molecular characterization: two case reports and update on diagnosis
Lijia Wen, Benhe Wang, Hui Zhang
Frontiers in Medicine.2025;[Epub] CrossRef
Parasitic Diseases of Upper Gastrointestinal Tract
Heung Up Kim
The Korean Journal of Helicobacter and Upper Gastrointestinal Research.2024; 24(2): 127. CrossRef
Rare Case of Echinostoma cinetorchis Infection, South Korea
Sooji Hong, Hyejoo Shin, Yoon-Hee Lee, Sung-Jong Hong, So-Ri Kim, Youn-Kyoung Kim, Young-Jin Son, Jeong-Gil Song, Jong-Yil Chai, Bong-Kwang Jung
Emerging Infectious Diseases.2024;[Epub] CrossRef
Neglected food-borne trematodiases: echinostomiasis and gastrodiscoidiasis
Rafael Toledo, María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli
Parasitology.2022; 149(10): 1319. CrossRef
Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
The Korean Journal of Parasitology.2020; 58(5): 499. CrossRef
Fasciola hepatica infection in children actively detected in a survey in rural areas of Mardan district, Khyber Pakhtunkhawa province, northern Pakistan
Asma W. Qureshi, Aurang Zeb, Abu Mansoor, Azam Hayat, Santiago Mas-Coma
Parasitology International.2019; 69: 39. CrossRef
Human echinostomiasis: a case report
Ranjit Sah, Shusila Khadka, Rabin Hamal, Sagar Poudyal
BMC Research Notes.2018;[Epub] CrossRef
Infection Status of Isthmiophora hortensis Metacercariae in Dark Sleepers, Odontobutis Species, from Some Water Systems of the Republic of Korea
Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho, Jung-Won Ju
The Korean Journal of Parasitology.2018; 56(6): 633. CrossRef
New Definitive Hosts and Differential Body Indices of Isthmiophora hortensis (Digenea: Echinostomatidae)
Woon-Mok Sohn, Byoung-Kuk Na, Sung-Shik Shin
The Korean Journal of Parasitology.2017; 55(3): 287. CrossRef
Echinostoma macrorchis (Digenea: Echinostomatidae): Metacercariae in Cipangopaludina chinensis malleata Snails and Adults from Experimental Rats in Korea
Woon-Mok Sohn, Byoung-Kuk Na
The Korean Journal of Parasitology.2017; 55(5): 541. CrossRef
Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study
Hudson A. Pinto, Matt J. Griffin, Sylvie M. Quiniou, Cynthia Ware, Alan L. Melo
Parasitology Research.2016; 115(1): 51. CrossRef
Epidemiological analysis of human fascioliasis in northeastern Punjab, Pakistan
Asma W. Qureshi, Akhtar Tanveer, Santiago Mas-Coma
Acta Tropica.2016; 156: 157. CrossRef
An update on human echinostomiasis
R. Toledo, J. G. Esteban
Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 37. CrossRef
Animal Models for Echinostoma malayanum Infection: Worm Recovery and Some Pathology
Jiraporn Songsri, Ratchadawan Aukkanimart, Thidarut Boonmars, Panaratana Ratanasuwan, Porntip Laummaunwai, Pranee Sriraj, Panupan Sripan
The Korean Journal of Parasitology.2016; 54(1): 47. CrossRef
Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae)
Ze-Xuan Liu, Yan Zhang, Yu-Ting Liu, Qiao-Cheng Chang, Xin Su, Xue Fu, Dong-Mei Yue, Yuan Gao, Chun-Ren Wang
The Korean Journal of Parasitology.2016; 54(2): 173. CrossRef
A Case of Echinostoma cinetorchis (Trematoda: Echinostomatidae) Infection Diagnosed by Colonoscopy
Woon Tae Jung, Kyeong Ju Lee, Hong Jun Kim, Tae Hyo Kim, Byoung-Kuk Na, Woon-Mok Sohn
The Korean Journal of Parasitology.2014; 52(3): 287. CrossRef
Echinostoma hortense Infection with Enteritis Diagnosed by Upper Gastrointestinal Endoscopy in a Dog
Hiroki OKANISHI, Jun MATSUMOTO, Sadao NOGAMI, Yumiko KAGAWA, Toshihiro WATARI
Journal of Veterinary Medical Science.2013; 75(7): 991. CrossRef
Evaluation of the role of rats as reservoir hosts for fishborne zoonotic trematodes in two endemic northern Vietnam fish farms
Nguyen Lan Anh Thi, Henry Madsen, Dao Thanh Ha, Eric Hoberg, Anders Dalsgaard, K. Darwin Murrell
Parasitology Research.2012; 111(3): 1045. CrossRef
Echinostoma hortense and Heterophyid Metacercariae Encysted in Yellowfin Goby, Acanthogobius flavimanus, from Shinan-gun and Muan-gun (Jeollanam-do), Korea
Woon-Mok Sohn, Byoung-Kuk Na, Shin-Hyeong Cho
The Korean Journal of Parasitology.2009; 47(3): 307. CrossRef
Foodborne Intestinal Flukes in Southeast Asia
Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
The Korean Journal of Parasitology.2009; 47(Suppl): S69. CrossRef
ECHINOSTOMA HORTENSE ASADA INFECTION IN THE DUODENUM: INCIDENTAL FINDINGS DURING ROUTINE GASTROINTESTINAL ENDOSCOPY
Toshiaki Tanaka, Tomoari Kamada, Hideki Koga, Aki Tanaka, Noriaki Manabe, Jiro Hata, Hiroaki Kusunoki, Manabu Ishii, Motonori Sato, Kenichi Tarumi, Akiko Shiotani, Tetsuya Okino, Ken Haruma
Digestive Endoscopy.2008; 20(2): 87. CrossRef
Eosinophilia: Causes and pathobiology in persons with prior exposures in tropical areas with an emphasis on parasitic infections
Yae-Jean Kim, Thomas B. Nutman
Current Infectious Disease Reports.2006; 8(1): 43. CrossRef
A human infection of Echinostoma hortense in duodenal bulb diagnosed by endoscopy
Young-Doo Chang, Woon-Mok Sohn, Jae-Hwa Ryu, Shin-Yong Kang, Sung-Jong Hong
The Korean Journal of Parasitology.2005; 43(2): 57. CrossRef

9,408 View
94 Download
Crossref

The intestinal parasite infection status of inhabitants in the Roxas city, the Philippines: Bong-Jin Kim, Mee-Sun Ock, Dong-Il Chung, Tai-Soon Yong, Kyu-Jae Lee; Korean J Parasitol 2003;41(2):113-115.
Published online June 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.2.113

Abstract
PDF

We carried out a small-scale survey to investigate the status of intestinal protozoa and helminthes infection of inhabitants in Roxas city, Mindoro, the Philippines. Total 301 stool samples were subjected to the formalin-ether concentration method for the detection of helminth ova and protozoan cysts. The overall positive rate was 64.5%, and that of male and female were 56.6% and 72.5%, respectively. The highest infected helminth was Ascaris lumbricoudes (51.2%), followed by Trichuris trichiura (27.6%), hookworm (8.0%) and Enterobius vermicularis (0.3%). The protozoa infection status revealed that Entamoeba coli was the most frequent (15.0%). Iodoamoeba buetschlii and E. histolytica were found but few. The multiple infection more than two parasites was 29.6%, and double infection with A. lumbricoides and T. trichiura was most common. The intestinal helminth infections were highly prevalent in this area, according to this result, and we concluded that anthelminthic drugs should be given to inhabitants, especially to children of 1 to 15 years-old.

Citations

Citations to this article as recorded by

Prevalence and Predictors of Intestinal Parasitic Infections at King Abdulaziz University Hospital, Jeddah, Saudi Arabia, from 2019 to 2023: A Retrospective Study
Shaymaa Abdalal, Hatoon Niyazi, Soa’ad Alsulami, Ala Azhari, Hanouf Niyazi, Jawahir Mokhtar, Dalya Attallah, Faten Al Braikan, Moroj Aldarmasi
Infection and Drug Resistance.2024; Volume 17: 2793. CrossRef
The control of soil-transmitted helminthiases in the Philippines: the story continues
Mary Lorraine S. Mationg, Veronica L. Tallo, Gail M. Williams, Catherine A. Gordon, Archie C. A. Clements, Donald P. McManus, Darren J. Gray
Infectious Diseases of Poverty.2021;[Epub] CrossRef
“STUDY OF THE PREVALENCE OF INTESTINAL PARASITES IN A LOW SOCIOECONOMIC AREA OF NORTH INDIA.”
Nandlal Kumar, Poonam Sagar, Preeti Sagar
INDIAN JOURNAL OF APPLIED RESEARCH.2021; : 60. CrossRef
Oreochromis niloticus and Clarias gariepinus Fish Infected with Digenean Metacercariae: Experimental Infection of Puppies and Some Chemical Alterations in Fish
Ghada A.K. Kirrella, Nagwa M. Elhawary, Eman K. Bazh, Shimaa S. Gh. Sorour, Khaled Mohamed El-Dakhly
Asian Journal of Animal and Veterinary Advances.2018; 13(4): 352. CrossRef
A STUDY OF INTESTINAL PARASITIC INFESTATIONS AMONG SCHOOL CHILDREN IN BAGEPALLI TALUK, CHIKKABALLAPUR DISTRICT, KARNATAKA- A CROSS- SECTIONAL SCHOOL SURVEY
Ramakrishna Reddy N, Riyaz Basha
Journal of Evolution of medical and Dental Sciences.2013; 2(10): 1416. CrossRef
Intestinal Parasitic Infection among School Children in Golestan Province, Iran
Rostami Masoumeh, Tohidi Farideh, Sharbatkhori Mitra, Taherkhani Heshmatoll
Pakistan Journal of Biological Sciences.2012; 15(23): 1119. CrossRef
The epidemiology of soil-transmitted helminth and protozoan infections in south-west Cameroon
J.V. Mbuh, N.H. Ntonifor, J. Ojong
Journal of Helminthology.2012; 86(1): 30. CrossRef
Epidemiological survey of the prevalence of intestinal parasites among schoolchildren in Sari, northern Iran
A. Daryani, M. Sharif, M. Nasrolahei, A. Khalilian, A. Mohammadi, Gh. Barzegar
Transactions of the Royal Society of Tropical Medicine and Hygiene.2012; 106(8): 455. CrossRef
Intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern Iran
Mehdi Sharif, Ahmad Daryani, Fatemeh Asgarian, Mohtaram Nasrolahei
Research in Developmental Disabilities.2010; 31(4): 924. CrossRef
Intestinal Parasitic Infections among Inhabitants of Karaj City, Tehran Province, Iran in 2006-2008
Vahid Nasiri, Kasra Esmailnia, Gholamreza Karim, Mehdi Nasir, Omid Akhavan
The Korean Journal of Parasitology.2009; 47(3): 265. CrossRef
Prevalence of Giardia lamblia with or without diarrhea in South East, South East Asia and the Far East
Hassan H. Dib, Si Qi Lu, Shao Fang Wen
Parasitology Research.2008; 103(2): 239. CrossRef
Comparison of three immunodiagnostic tests for experimental Heterophyes heterophyes infection in dogs
Attef M. Elshazly, Hany M. Elsheikha, Mohammad H. Rahbar, Soha I. Awad
Veterinary Parasitology.2008; 151(2-4): 196. CrossRef
Large-Scale Preventive Chemotherapy for the Control of Helminth Infection in Western Pacific Countries: Six Years Later
Antonio Montresor, Dai Tran Cong, Mouth Sinuon, Reiko Tsuyuoka, Chitsavang Chanthavisouk, Hanne Strandgaard, Raman Velayudhan, Corinne M. Capuano, Tuan Le Anh, Ah S. Tee Dató, Simon Brooker
PLoS Neglected Tropical Diseases.2008; 2(8): e278. CrossRef
Infection status of intestinal parasites in children living in residential institutions in Metro Manila, the Philippines
Eleonor T Baldo, Vicente Y Belizario, Winifreda U De Leon, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2004; 42(2): 67. CrossRef

9,560 View
155 Download
Crossref

Original Articles

Cloning and characterization of Giardia intestinalis cyclophilin: Hak-Sun Yu, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2002;40(3):131-138.
Published online September 30, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.3.131

Abstract
PDF

The cyclophilins (Cyps) are family members of proteins that exhibit peptidyl-prolyl cis-trans isomerase (PPIase, EC 5.2.1.8) activity and bind the immunosuppressive agent cyclosprin A (CsA) in varying degrees. During the process of random sequencing of a cDNA library made from Giardia intestinalis WB strain, the cyclophilin gene (gicyp1) was isolated. An open reading frame of gicyp1 gene was 576 nucleotides, which corresponded to a translation product of 176 amino acids (Gicyp1). The identity with other Cyps was about 58-71%. The 13 residues that constituted the CsA binding site of human cyclophilin were also detected in the amino acid sequence of Gicyp1, including tryptophan residue essential for the drug binding. The single copy of the gicyp1 gene was detected in the G. intestinalis chromosome by southern hybridization analysis. Recombinant Gicyp1 protein clearly accelerated the rate of cis → trans isomerization of the peptide substrate and the catalysis was completely inhibited by the addition of 0.5 ?M CsA.

Citations

Citations to this article as recorded by

Cyclophilins as key players in protozoan parasite infections
Reza Mansouri, Enrique Granado-Aparicio, Claudia Alcedo, Julio López-Abán, Reza Shafiei, Antonio Muro, Raúl Manzano-Román, Sajad Rashidi
Parasites & Vectors.2025;[Epub] CrossRef
Insights into Peptidyl-Prolyl cis-trans Isomerases from Clinically Important Protozoans: From Structure to Potential Biotechnological Applications
Verónica Aranda-Chan, Rosa Elena Cárdenas-Guerra, Alejandro Otero-Pedraza, Esdras Enoc Pacindo-Cabrales, Claudia Ivonne Flores-Pucheta, Octavio Montes-Flores, Rossana Arroyo, Jaime Ortega-López
Pathogens.2024; 13(8): 644. CrossRef
Molecular aspects of cyclophilins mediating therapeutic actions of their ligands
Andrzej Galat, Jacqueline Bua
Cellular and Molecular Life Sciences.2010; 67(20): 3467. CrossRef
Peptidyl-prolyl cis–trans isomerases (immunophilins) and their roles in parasite biochemistry, host–parasite interaction and antiparasitic drug action
Angus Bell, Paul Monaghan, Antony P. Page
International Journal for Parasitology.2006; 36(3): 261. CrossRef
A Cyclophilin from Griffithsia japonica Has Thermoprotective Activity and Is Affected by CsA
Eun Kyung Cho, Yoo Kyung Lee, Choo Bong Hong
Molecules and Cells.2005; 20(1): 142. CrossRef

8,036 View
81 Download
Crossref

A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level: Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2002;40(1):25-31.
Published online March 31, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.1.25

Abstract
PDF

We describe a riboprinting scheme for identification of unknown Acanthamoeba isolates at the species level. It involved the use of PCR-RFLP of small subunit ribosomal RNA gene (riboprint) of 24 reference strains by 4 kinds of restriction enzymes. Seven strains in morphological group I and III were identified at species level with their unique sizes of PCR product and riboprint type by Rsa I. Unique RFCP of 17 strains in group II by Dde I, Taq I and Hae III were classified into: (1) four taxa that were identifiable at the species level, (2) a subgroup of 4 taxa and a pair of 2 taxa that were identical with each other, and (3) a species complex of 7 taxa assigned to A. castellanii complex that were closely related. These results were consistent with those obtained by 18s rDNA sequence analysis. This approach provides an alternative to the rDNA sequencing for rapid identification of a new clinical isolate or a large number of environmental isolates of Acanthamoeba.

Citations

Citations to this article as recorded by

Pathogenic free-living amoebae in Korea
Ho-Joon Shin, Kyung-il Im
The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef
Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish
Kyung-il Im, Ho-Joon Shin
The Korean Journal of Parasitology.2003; 41(4): 181. CrossRef
Acanthamoebaspp. as Agents of Disease in Humans
Francine Marciano-Cabral, Guy Cabral
Clinical Microbiology Reviews.2003; 16(2): 273. CrossRef

8,618 View
61 Download
Crossref

Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi: Yeon-Chul Hong, Mi-Yul Hwang, Ho-Cheol Yun, Hak-Sun Yu, Hyun-Hee Kong, Tai-Soon Yong, Dong-Il Chung; Korean J Parasitol 2002;40(1):17-24.
Published online March 31, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.1.17

Abstract
PDF

We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an A. healyi cDNA library screening was amplified by PCR using degenerate oligonucleotide primers designed on the basis of conserved amino acids franking the active sites of cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteinases. Cysteine proteinase gene of A. healyi (AhCP1) was composed of 330 amino acids with signal sequence, a proposed pro-domain and a predicted active site made up of the catalytic residues, Cys²⁵, His¹⁵⁹, and Asn¹⁷⁵. Deduced amino acid sequence analysis indicates that AhCP1 belong to ERFNIN subfamily of C1 peptidases. By Northern blot analysis, no direct correlation was observed between AhCP1 mRNA expression and virulence of Acanthamoeba, but the gene was expressed at higher level in amoebae isolated from soil than amoeba from clinical samples. These findings raise the possibility that Ahcp1 protein may play a role in protein metabolism and digestion of phagocytosed bacteria or host tissue debris rather than in invasion of amoebae into host tissue.

Citations

Citations to this article as recorded by

Amoebic encephalitis within Australia
Samantha C. Carija, Avram Levy, Graham Weaire‐Buchanan, Terence Lee, Robin Woodward, Jake Gazeley, Troy A. Edwards, Jason M. Dyke, Vicki Fabian, Katherine Norton, Andrew Chapman, Paul R. Ingram
Internal Medicine Journal.2025; 55(1): 143. CrossRef
Biological characteristics and pathogenicity of Acanthamoeba
Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, Mingguang Li
Frontiers in Microbiology.2023;[Epub] CrossRef
Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zhixin Wang, Duo Wu, Hiroshi Tachibana, Meng Feng, Xun-jia Cheng
Parasites & Vectors.2020;[Epub] CrossRef
Anti-amoebic potential of azole scaffolds and nanoparticles against pathogenic Acanthamoeba
Shweta Walvekar, Ayaz Anwar, Areeba Anwar, Nanthini Sridewi, Mohammad Khalid, Yoon Yen Yow, Naveed Ahmed Khan
Acta Tropica.2020; 211: 105618. CrossRef
Host Invasion by Pathogenic Amoebae: Epithelial Disruption by Parasite Proteins
Abigail Betanzos, Cecilia Bañuelos, Esther Orozco
Genes.2019; 10(8): 618. CrossRef
Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii
Yeonchul Hong, Jung-Mi Kang, So-Young Joo, Su-Min Song, Hương Giang Lê, Thị Lam Thái, Jinyoung Lee, Youn-Kyoung Goo, Dong-Il Chung, Woon-Mok Sohn, Byoung-Kuk Na
The Korean Journal of Parasitology.2018; 56(5): 409. CrossRef
Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba
Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Hyun-Hee Kong
Molecular and Biochemical Parasitology.2012; 185(2): 121. CrossRef
Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity
Jenica L. Harrison, Gabriela A. Ferreira, Erinn S. Raborn, Audrey D. Lafrenaye, Francine Marciano-Cabral, Guy A. Cabral
Infection and Immunity.2010; 78(9): 4001. CrossRef
Characterization of a Serine Proteinase Mediating Encystation of Acanthamoeba
Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong
Eukaryotic Cell.2008; 7(9): 1513. CrossRef
Differentially expressed genes of Acanthamoeba castellanii during encystation
Eun-Kyung Moon, Dong-Il Chung, Yeon-Chul Hong, Hyun-Hee Kong
The Korean Journal of Parasitology.2007; 45(4): 283. CrossRef
Acanthamoeba: biology and increasing importance in human health
Naveed Ahmed Khan
FEMS Microbiology Reviews.2006; 30(4): 564. CrossRef
Intracellular Localization and Trafficking of Serine Proteinase AhSub and Cysteine Proteinase AhCP of Acanthamoeba healyi
E.-K. Moon, S.-T. Lee, D.-I. Chung, H.-H. Kong
Eukaryotic Cell.2006; 5(1): 125. CrossRef
Pathogenic free-living amoebae in Korea
Ho-Joon Shin, Kyung-il Im
The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef

8,101 View
96 Download
Crossref

Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea: Hak-Sun Yu, Kyung-Hee Choi, Hyo-Kyung Kim, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2001;39(2):161-170.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.161

Abstract
PDF

We conducted both the small subunit ribosomal DNA (SSU rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and mitochondrial (mt) DNA RFLP analyses for a genetic characterization of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea. Twenty-three strains of Acanthamoeba from the American Type Culture Collection and twelve clinical isolates from Korean patients were used as reference strains. Thirty-nine isolates from contact lens storage cases were classified into seven types (KA/LS1, KA/LS2, KA/LS4, KA/LS5, KA/LS7, KA/LS18, KA/LS31). Four types (KA/LS1, KA/LS2, KA/LS5, KA/LS18) including 33 isolates were regarded as A. castellanii complex by riboprints. KA/LS1 type was the most predominant (51.3%) in the present survey area, followed by KA/LS2 (20.9%), and KA/LS5 (7.7%) types. Amoebae of KA/LS1 type had the same mtDNA RFLP and riboprint patterns as KA/E2 and KA/E12 strains, clinical isolates from Korean keratitis patients. Amoebae of KA/LS2 type had the identical mtDNA RFLP patterns with A. castellanii Ma strain, a corneal isolate from an American patient as amoebae of KA/LS5 type, with KA/E3 and KA/E8 strains from other Korean keratitis patients. Amoebae of KA/LS18 type had identical patterns with JAC/E1, an ocular isolate from a Japanese patient. Three types, which remain unidentified at species level, were not corresponded with any clinical isolate in their mtDNA RFLP and riboprint patterns. Out of 39 isolates analyzed in this study, mtDNA RFLP and riboprint patterns of 33 isolates (84.6%) were identical to already known clinical isolates, and therefore, they may be regarded as potentially keratopathogenic. These results suggest that contact lens wearers in Seoul should pay more attention to hygienic maintenance of contact lens storage cases for the prevention of Acanthamoeba keratitis.

Citations

Citations to this article as recorded by

Parasitological and microbiological assessment of contact lens storage cases: a survey of asymptomatic lens student wearers from five medical specialties in Tunisia, North Africa
Sameh Belgacem, Raja Chaâbane-Banaoues, Amira Mejri, Sawsen Ben Ifa, Maha Mastouri, Hamouda Babba
BMC Infectious Diseases.2025;[Epub] CrossRef
The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis
Min Seung Kang, Sangyoon Kim, Da Som Kim, Hak Sun Yu, Ji Eun Lee, Yi Cao
PLOS ONE.2023; 18(2): e0281141. CrossRef
The First Case of Cutaneous Acanthamoebiasis Caused by Acanthamoeba triangularis in Korea
Mi Soo Choi, Na Hye Myong, Min Seo, Sukbin Jang, Dae Kwan Yun, Kyujin Yeom, Dong-Il Chung, Byung Cheol Park, Yeonchul Hong, Myung Hwa Kim
Annals of Dermatology.2023; 35(Suppl 2): S275. CrossRef
Adhesion ofAcanthamoebaon Cosmetic Contact Lenses
Seung-Mok Lee, Ji-Eun Lee, Da-In Lee, Hak-Sun Yu
Journal of Korean Medical Science.2018;[Epub] CrossRef
Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
Angela Magnet, Thiago Santos Gomes, Carmen Pardinas, Natalia Garcia de Blas, Cruz Sadaba, Eugenia Carrillo, Fernando Izquierdo, José Manuel Benítez del Castillo, Carolina Hurtado, Carmen del Aguila, Soledad Fenoy
Parasites & Vectors.2018;[Epub] CrossRef
Effect of Multipurpose Solution Combined With Autophagy Inhibitors on Adhesion of Acanthamoeba trophozoites to Silicone Hydrogel Contact Lenses
Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee
Cornea.2017; 36(12): 1538. CrossRef
Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses
Ga‐Hyun Lee, Hak‐Sun Yu, Ji‐Eun Lee
Ophthalmic and Physiological Optics.2016; 36(2): 93. CrossRef
Adhesion of Acanthamoeba on Silicone Hydrogel Contact Lenses
Ga-Hyun Lee, Ji-Eun Lee, Mi-Kyung Park, Hak-Sun Yu
Cornea.2016; 35(5): 663. CrossRef
Acanthamoeba spp. in Contact Lenses from Healthy Individuals from Madrid, Spain
Thiago dos Santos Gomes, Angela Magnet, Fernando Izquierdo, Lucianna Vaccaro, Fernando Redondo, Sara Bueno, Maria Luisa Sánchez, Santiago Angulo, Soledad Fenoy, Carolina Hurtado, Carmen del Aguila, Graham R. Wallace
PLOS ONE.2016; 11(4): e0154246. CrossRef
18S ribosomal DNA genotypes of Acanthamoeba species isolated from contact lens cases in the Philippines
Windell L. Rivera, Davin Edric V. Adao
Parasitology Research.2009; 105(4): 1119. CrossRef
Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea
Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu
The Korean Journal of Parasitology.2009; 47(4): 337. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
Detection of Acanthamoeba in Tap Water and Contact Lens Cases Using Polymerase Chain Reaction
Maureen Boost, Pauline Cho, Sindy Lai, Wing-Man Sun
Optometry and Vision Science.2008; 85(7): 526. CrossRef
Acanthamoeba spp. and bacterial contamination in contact lens storage cases and the relationship to user profiles
Claiton José Pens, Marisa da Costa, Cristina Fadanelli, Karin Caumo, MariliseBrittes Rott
Parasitology Research.2008; 103(6): 1241. CrossRef
Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef
Distribution of Acanthamoeba spp. in Raw Water and Water Treatment Process

Journal of the Environmental Sciences.2008; 17(10): 1121. CrossRef
Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients
Ying-Hua Xuan, Hak Sun Yu, Hae Jin Jeong, Sung-Yong Seol, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2007; 45(1): 1. CrossRef
Cysticidal Effect on Acanthamoeba and Toxicity on Human Keratocytes by Polyhexamethylene Biguanide and Chlorhexidine
Ji-Eun Lee, Boo Sup Oum, Hee Young Choi, Hak Sun Yu, Jong Soo Lee
Cornea.2007; 26(6): 736. CrossRef
Acanthamoeba: Keratopathogenicity of isolates from domestic tap water in Korea
Hae Jin Jeong, Sun Joo Lee, Jeong Hwan Kim, Ying Hua Xuan, Keun Hee Lee, Sang Kyun Park, Sun Hee Choi, Dong Il Chung, Hyun Hee Kong, Mee Sun Ock, Hak Sun Yu
Experimental Parasitology.2007; 117(4): 357. CrossRef
Factors Affecting the Epidemiology ofAcanthamoebaKeratitis
Youhanna W. Ibrahim, David L. Boase, Ian A. Cree
Ophthalmic Epidemiology.2007; 14(2): 53. CrossRef
Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
Sun Joo Lee, Hae Jin Jeong, Ji Eun Lee, Jong Soo Lee, Ying Hua Xuan, Hyun-Hee Kong, Dong-Il Chung, Mee-Sun Ock, Hak Sun Yu
The Korean Journal of Parasitology.2006; 44(4): 313. CrossRef
The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea
Hae Jin Jeong, Hak Sun Yu
The Korean Journal of Parasitology.2005; 43(2): 47. CrossRef
Acanthamoeba keratitis update—incidence, molecular epidemiology and new drugs for treatment
D V Seal
Eye.2003; 17(8): 893. CrossRef
Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small-Subunit Ribosomal DNA PCR-RFLP Analyses ofAcanthamoebaIsolated from Contact Lens Storage Cases of Residents in Southwestern Korea
Hyun-Hee Kong, Ji-Yeol Shin, Hak-Sun Yu, Jin Kim, Tae-Won Hahn, Young-Ho Hahn, Dong-Il Chung
Journal of Clinical Microbiology.2002; 40(4): 1199. CrossRef

9,213 View
53 Download
Crossref

Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi: Hyun-Hee Kong, Mee-Yeul Hwang, Hyo-Kyung Kim, Dong-Il Chung; Korean J Parasitol 2001;39(2):151-160.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.151

Abstract
PDF

Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.

Citations

Citations to this article as recorded by

Construction of EST Database for Comparative Gene Studies of Acanthamoeba
Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2009; 47(2): 103. CrossRef
Acanthamoeba Interaction with Extracellular Matrix Glycoproteins: Biological and Biochemical Characterization and Role in Cytotoxicity and Invasiveness
BRUNO DA ROCHA‐AZEVEDO, MELISSA JAMERSON, GUY A. CABRAL, FERNANDO C. SILVA‐FILHO, FRANCINE MARCIANO‐CABRAL
Journal of Eukaryotic Microbiology.2009; 56(3): 270. CrossRef
Sampling Gene Diversity Across the Supergroup Amoebozoa: Large EST Data Sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp.
Russell F. Watkins, Michael W. Gray
Protist.2008; 159(2): 269. CrossRef
Anisakis simplex: Analysis of expressed sequence tags (ESTs) of third-stage larva
Hak Sun Yu, Sang Kyun Park, Keun Hee Lee, Sun Joo Lee, Sun Hee Choi, Mee Sun Ock, Hae Jin Jeong
Experimental Parasitology.2007; 117(1): 51. CrossRef
Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba
Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2006; 44(4): 331. CrossRef
Acanthamoeba healyi: Molecular cloning and characterization of a coronin homologue, an actin-related protein
Eleonor T. Baldo, Eun-Kyung Moon, Hyun-Hee Kong, Dong-Il Chung
Experimental Parasitology.2005; 110(2): 114. CrossRef

7,820 View
55 Download
Crossref

Brief Communication

The first human case of Trichinella spiralis infection in Korea: Woon-Mok Sohn, Han-Mo Kim, Dong-Il Chung, Sung Tae Yee; Korean J Parasitol 2000;38(2):111-115.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.111

Abstract
PDF

Three cases of human infection by Trichinella spiralis were first confirmed by detecting encysted larvae in the biopsied muscle in December 1997, in Korea. The patients were one 35- and two 39-year-old males residing in Kochang-gun, Kyongsangnam-do. They had a common past history of eating raw liver, spleen, blood and muscle of a badger, Meles meles melanogenys, and complained of high fever, facial and periorbital edema, and myalgia. Hematologic and biochemical examinations revealed leukocytosis and eosinophilia, and highly elevated levels of GOT, GPT, LDH and CPK. In the gastrocnemius muscle of a patient, roundly coiled nematode larvae were detected. The larvae measured 0.775-1.050 (av. 0.908) mm in length, and 0.026-0.042 (av. 0.035) mm in maximum width. The specific IgG antibody levels in three patients' sera were significantly higher when compared with those of normal controls. The patients were treated with flubendazole and albendazole for 15-30 days, and discharged at 13-34 days post-admission. From the above findings, it was confirmed that T. spiralis is present in Korea, and the badger plays a role of as the natural host.

Citations

Citations to this article as recorded by

From wildlife to humans: The global distribution of Trichinella species and genotypes in wildlife and wildlife-associated human trichinellosis
Cody J. Malone, Antti Oksanen, Samson Mukaratirwa, Rajnish Sharma, Emily Jenkins
International Journal for Parasitology: Parasites and Wildlife.2024; 24: 100934. CrossRef
Human parasitic infections of the class Adenophorea: global epidemiology, pathogenesis, prevention and control
Jitrawadee Intirach, Chang Shu, Xin Lv, Suzhen Gao, Nataya Sutthanont, Tao Chen, Zhiyue Lv
Infectious Diseases of Poverty.2024;[Epub] CrossRef
Wild Mesocarnivores as Reservoirs of Endoparasites Causing Important Zoonoses and Emerging Bridging Infections across Europe
Fabrizia Veronesi, Georgiana Deak, Anastasia Diakou
Pathogens.2023; 12(2): 178. CrossRef
Wildmeat consumption and zoonotic spillover: contextualising disease emergence and policy responses
Charlotte Milbank, Bhaskar Vira
The Lancet Planetary Health.2022; 6(5): e439. CrossRef
Prevalence of meat-transmitted Taenia and Trichinella parasites in the Far East countries
Yi Liu, Zijian Dong, Jianda Pang, Mingyuan Liu, Xuemin Jin
Parasitology Research.2021; 120(12): 4145. CrossRef
Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide
Xiaoli Wang, Liang Li, Xing Wei, Yuanyuan Wang, Hui Zhang, Ao Shi, Tao Liu, Xiaodi Yang, Qiang Fang, Linsheng Song
PLOS ONE.2018; 13(6): e0198205. CrossRef
Meat sources of infection for outbreaks of human trichinellosis
Ali Rostami, H. Ray Gamble, Jean Dupouy-Camet, Hooshang Khazan, Fabrizio Bruschi
Food Microbiology.2017; 64: 65. CrossRef
Prevalence of Trichinella spp. antibodies in wild boars (Sus scrofa) and domestic pigs in Korea
H.J. Kim, W.S. Jeong, E.M. Kim, S.G. Yeo, D.J. An, H. Yoon, E.J. Kim, C.K. Park
Veterinární medicína.2015; 60(4): 181. CrossRef
Prevalence of Antibodies toToxoplasma gondiiin South Korean Wild Boar (Sus scrofa coreanus)
Wooseog Jeong, Hachung Yoon, Yong Kwan Kim, Oun-kyong Moon, Do-Soon Kim, Dong-Jun An
Journal of Wildlife Diseases.2014; 50(4): 902. CrossRef
Chemotherapeutic drugs for common parasitic diseases in Korea
Sun Huh
Journal of the Korean Medical Association.2013; 56(6): 513. CrossRef
Clinical Update on Parasitic Diseases
Min Seo
Korean Journal of Medicine.2013; 85(5): 469. CrossRef
Trichinosis Caused by Ingestion of Raw Soft-Shelled Turtle Meat in Korea
Sang-Rok Lee, Sang-Hoon Yoo, Hyun-Seon Kim, Seung-Ha Lee, Min Seo
The Korean Journal of Parasitology.2013; 51(2): 219. CrossRef
An Outbreak of Trichinellosis with Detection ofTrichinellaLarvae in Leftover Wild Boar Meat
Gayeon Kim, Min-Ho Choi, Jae-Hwan Kim, Yu Min Kang, Hee Jung Jeon, Younghee Jung, Myung Jin Lee, Myoung-don Oh
Journal of Korean Medical Science.2011; 26(12): 1630. CrossRef
The Fifth Outbreak of Trichinosis in Korea
Ji-Young Rhee, Sung-Tae Hong, Hye-Jung Lee, Min Seo, Suk-Bae Kim
The Korean Journal of Parasitology.2011; 49(4): 405. CrossRef
Pathogenesis of restricted movements in trichinellosis: An experimental study
Dalia S. Ashour, Reda H. Elbakary
Experimental Parasitology.2011; 128(4): 414. CrossRef
The epidemiology of human trichinellosis in China during 2004–2009
J. Cui, Z.Q. Wang, B.L. Xu
Acta Tropica.2011; 118(1): 1. CrossRef
Recent Advances in the Use of Anthelmintics for Treating Nematode Infections
Jong-Yil Chai
Infection and Chemotherapy.2011; 43(1): 26. CrossRef
Changing Patterns of Human Parasitic Infection in Korea
Myoung-Hee Ahn
Hanyang Medical Reviews.2010; 30(3): 149. CrossRef
Human Trichinosis after Consumption of Soft-Shelled Turtles, Taiwan
Yi-Chun Lo, Chien-Ching Hung, Ching-Shih Lai, Zhiliang Wu, Isao Nagano, Takuya Maeda, Yuzo Takahashi, Chan-Hsien Chiu, Donald Dah-Shyong Jiang
Emerging Infectious Diseases.2009; 15(12): 2056. CrossRef
Fifty Years of the Korean Society for Parasitology
Seung-Yull Cho
The Korean Journal of Parasitology.2009; 47(Suppl): S7. CrossRef
Review of Zoonotic Parasites in Medical and Veterinary Fields in the Republic of Korea
Heejeong Youn
The Korean Journal of Parasitology.2009; 47(Suppl): S133. CrossRef
Trends in parasitic diseases in the Republic of Korea
Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
Trends in Parasitology.2008; 24(3): 143. CrossRef
Food-borne parasitic zoonosis: Distribution of trichinosis in Thailand
Natthawut Kaewpitoon, Soraya Jatesadapattaya Kaewpitoon, Prasit Pengsaa
World Journal of Gastroenterology.2008; 14(22): 3471. CrossRef
Tissue Invading Helminthic Diseases
Yoon Kong
Journal of the Korean Medical Association.2007; 50(11): 967. CrossRef
World distribution of Trichinella spp. infections in animals and humans
Edoardo Pozio
Veterinary Parasitology.2007; 149(1-2): 3. CrossRef
Emerging Parasitic Diseases in Korea
Jong-Yil Chai
Journal of the Korean Medical Association.2007; 50(11): 946. CrossRef
Food-Borne Parasitic Diseases
Han-Jong Rim
Journal of the Korean Medical Association.2007; 50(11): 984. CrossRef
Trichinosis: Epidemiology in Thailand
Natthawut Kaewpitoon
World Journal of Gastroenterology.2006; 12(40): 6440. CrossRef
Food-borne Parasitic Diseases
Jong-Yil Chai
Journal of the Korean Medical Association.2004; 47(6): 496. CrossRef
Molecular identification of Korean Trichinella isolates
Woon-Mok Sohn, Sun Huh, Dong-Il Chung, Edoardo Pozio
The Korean Journal of Parasitology.2003; 41(2): 125. CrossRef
PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region
Hye Soo Kwon, Myung Sook Chung, Kyoung Hwan Joo
The Korean Journal of Parasitology.2001; 39(1): 43. CrossRef
Enzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea
Sung-Hwan Wee, Chung-Gil Lee, Hoo-Don Joo, Yung-Bai Kang
The Korean Journal of Parasitology.2001; 39(3): 261. CrossRef

20,803 View
129 Download
Crossref

Original Article

Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene: Hak-Sun Yu, Mee-Yul Hwang, Tae-Ook Kim, Ho-Cheol Yun, Tae-Ho Kim, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 1999;37(3):181-188.
Published online September 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.3.181

Abstract
PDF

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close interrelationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18S rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18S riboprinting. Acanthamoeba griffini of morphological group 2 branched between pathogenic (A. culbertsoni A-1 and A. healyi OC-3A) and nonpathogenic (A. palestinensis Reich, A. pustulosa GE-3a, A. royreba Oak Ridge, and A lenticulata PD2S) strains of morphological group 3. Among the remaining isolates of morphological group 2, the Chang strain had the identical mitochondrial riboprints as the type strain of A. hatchetti. AA2 and AA1, the type strains of A. divionensis and A. paradivionensis, respectively, had the identical riboprints as A. quina Vil3 and A. castellanii Ma. Although the branching orders of A. castellanii Neff, A. polyphaga P23, A. triangularis SH621, and A. lugdunensis L3a were different from those in 18S riboprinting analysis, the results obtained from this study generally coincided well with those from 18S riboprinting. Mitochondrial riboprinting may have an advantage over nuclear 18S rDNA riboprinting because the mt SSU rDNAs do not seem to have introns that are found in the 18S genes of Acanthamoeba and that distort phylogenetic analyses.

Citations

Citations to this article as recorded by

Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?
Merve Kahraman, Zübeyda Akın Polat
Turkish Journal of Parasitology.2024; 48(1): 15. CrossRef
The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Paul A. Fuerst
Microorganisms.2023; 11(2): 295. CrossRef
Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes
Daniele Corsaro
Microorganisms.2022; 10(11): 2162. CrossRef
DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia
Inese Gavarāne, Jūlija Trofimova, Artjoms Mališevs, Olga Valciņa, Muza Kirjušina, Ilze Rubeniņa, Aivars Bērziņš
Parasitology Research.2018; 117(10): 3299. CrossRef
Genotyping of Acanthamoeba T15: the environmental strain in Turkey
G. Evyapan, I. S. Koltas, F. Eroglu
Transactions of the Royal Society of Tropical Medicine and Hygiene.2015; 109(3): 221. CrossRef
Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef
Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2005; 43(1): 7. CrossRef
Pathogenic free-living amoebae in Korea
Ho-Joon Shin, Kyung-il Im
The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef
A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level
Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2002; 40(1): 25. CrossRef
Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge
Jill M. Schroeder, Gregory C. Booton, John Hay, Ingrid A. Niszl, David V. Seal, Miles B. Markus, Paul A. Fuerst, Thomas J. Byers
Journal of Clinical Microbiology.2001; 39(5): 1903. CrossRef
Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp
Julia Walochnik, Andreas Obwaller, Horst Aspöck
Applied and Environmental Microbiology.2000; 66(10): 4408. CrossRef