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"Haplorchis taichui"

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"Haplorchis taichui"

Original Articles

Infection Status of Zoonotic Trematode Metacercariae in Fishes from Vientiane Municipality and Champasak Province in Lao PDR
Keeseon S. Eom, Han-Sol Park, Dongmin Lee, Woon-Mok Sohn, Tai-Soon Yong, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Bounnaloth Insisiengmay, Bounlay Phommasack
Korean J Parasitol 2015;53(4):447-453.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.447
The infection status of fishborne zoonotic trematode (FZT) metacercariae was investigated in fishes from 2 localities of Lao PDR. Total 157 freshwater fishes (17 species) were collected in local markets of Vientiane Municipality and Champasak Province in December 2010 and July 2011, and each fish was examined by the artificial digestion method. Total 6 species of FZT metacercariae, i.e., Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium, were detected in fishes from Vientiane Municipality. The metacercariae of O. viverrini were detected in 50 (49.5%) out of 101 fishes (6 species), and their average number was 154 per fish infected. The remaining 5 species of heterophyid metacercariae were detected in 36.8%, 65.8%, 9.4%, 23.9%, and 5.1% fishes examined, and their average densities were 12, 1,038, 4, 15, and 13 per infected fish, respectively. In fishes from Champasak Province, 3 species of FZT metacercariae, i.e., O. viverrini, H. taichui, and H. yokogawai, were detected. Only 2 O. viverrini metacercariae were found in only 1 Barbonymus schwanefeldi. The metacercariae of H. taichui and H. yokogawai were detected in 60.0% and 50.0% of fishes examined, and their average densities were 47 and 28 per fish infected. By the present study, it has been confirmed that several species of FZT metacercariae are prevalent in fishes from Vientiane Municipality, with P. varium being a new member of FZT in Lao PDR. In comparison, FZT metacercariae are less prevalent in fishes from Champasak Province.

Citations

Citations to this article as recorded by  Crossref logo
  • Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
    Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
    EFSA Journal.2024;[Epub]     CrossRef
  • Infection rate of Opisthorchis viverrini metacercariae in cyprinoid fish from the markets and its association to human opisthorchiasis in the local community in the Northeast Thailand
    Lakhanawan Charoensuk, Alexis Ribas, Kacha Chedtabud, Suksanti Prakobwong
    Acta Tropica.2022; 225: 106216.     CrossRef
  • Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus
    Maria Y. Pakharukova, Viatcheslav A. Mordvinov
    Parasitology.2022; 149(10): 1306.     CrossRef
  • Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism
    Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli
    Animals.2020; 10(3): 456.     CrossRef
  • Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar
    Ei Ei Phyo Myint, Amornpun Sereemaspun, Joacim Rocklöv, Choosak Nithikathkul
    International Journal of Environmental Research and Public Health.2020; 17(11): 4108.     CrossRef
  • Larval Gnathostomes and Zoonotic Trematode Metacercariae in Fish from a Local Market in Yangon City, Myanmar
    Jong-Yil Chai, Bong-Kwang Jung, Keon Hoon Lee, Jin-Youp Ryu, Hyeon-Seung Kim, Sung-Jong Hong, Thi Thi Htoon, Htay Htay Tin, Byoung-Kuk Na, Woon-Mok Sohn
    The Korean Journal of Parasitology.2020; 58(6): 701.     CrossRef
  • Infection status with zoonotic trematode metacercariae in fish from Lao PDR
    Jong-Yil Chai, Soon-Hyung Lee, Han-Jong Rim, Woon-Mok Sohn, Bounlay Phommasack
    Acta Tropica.2019; 199: 105100.     CrossRef
  • Ultrasonographic investigation of cholangiocarcinoma in Lao PDR
    Ju Yeong Kim, Tai-Soon Yong, Han-Jong Rim, Jong-Yil Chai, Duk-Young Min, Keeseon S. Eom, Woon-Mok Sohn, Jae Hoon Lim, Dongil Choi, Sithat Insisiengmay, Bounlay Phommasack, Bounnaloth Insisiengmay
    Acta Tropica.2018; 182: 128.     CrossRef
  • Opisthorchis viverrini infection in the snail and fish intermediate hosts in Central Vietnam
    Ha Thanh Thi Dao, Veronique Dermauw, Sarah Gabriël, Apiporn Suwannatrai, Smarn Tesana, Giang Thanh Thi Nguyen, Pierre Dorny
    Acta Tropica.2017; 170: 120.     CrossRef
  • Metacercaria Infection Status of Fishborne Zoonotic Trematodes, Except for Clonorchis sinensis in Fish from the Heilongjiang Province, China
    Jian-Hua Qiu, Ying Zhang, Xiao-Xuan Zhang, Yuan Gao, Qi Li, Qiao-Cheng Chang, Chun-Ren Wang
    Foodborne Pathogens and Disease.2017; 14(8): 440.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2
    Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai
    The Korean Journal of Parasitology.2017; 55(4): 445.     CrossRef
  • Zoonotic Trematode Metacercariae in Fish from Yangon, Myanmar and Their Adults Recovered from Experimental Animals
    Jong-Yil Chai, Woon-Mok Sohn, Byoung-Kuk Na, Jong-Bok Park, Hoo-Gn Jeoung, Eui-Hyug Hoang, Thi Thi Htoon, Htay Htay Tin
    The Korean Journal of Parasitology.2017; 55(6): 631.     CrossRef
  • 11,206 View
  • 131 Download
  • 13 Web of Science
  • Crossref
Intestinal Helminths Recovered from Humans in Xieng Khouang Province, Lao PDR with a Particular Note on Haplorchis pumilio Infection
Jong-Yil Chai, Woon-Mok Sohn, Bong-Kwang Jung, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Bounnaloth Insisiengmay, Sithat Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2015;53(4):439-445.
Published online August 25, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.4.439
A survey of intestinal helminths was undertaken in riparian people in Xieng Khouang Province, Lao PDR. Fecal specimens were collected from 643 people (289 males and 354 females) residing in 4 districts (Nonghet, Kham, Phoukout, and Pek) and were examined by the Kato-Katz technique. The overall helminth egg positive rate was 41.2%, and hookworms revealed the highest prevalence (32.7%) followed by Trichuris trichiura (7.3%) and Ascaris lumbricoides (5.6%). The positive rate for small trematode eggs (STE), which may include Opisthorchis viverrini, heterophyids, and lecithodendriids, was 4.4%. For recovery of adult helminths, 12 STE or nematode/cestode egg-positive people were treated with 40 mg/kg praziquantel and 15 mg/kg pyrantel pamoate, and then purged. Mixed infections with 2 Haplorchis species (H. pumilio and H. taichui), Centrocestus formosanus, Opisthorchis viverrini, a species of cestode (Taenia saginata), and several species of nematodes including hookworms and Enterobius vermicularis were detected. The worm load for trematodes was the highest for H. pumilio with an average of 283.5 specimens per infected person followed by C. formosanus, H. taichui, and O. viverrini. The worm load for nematodes was the highest for hookworms (21.5/infected case) followed by E. vermicularis (3.2/infected case). The results revealed that the surveyed areas of Xieng Khouang Province, Lao PDR are endemic areas of various species of intestinal helminths. The STE found in the surveyed population were verified to be those of heterophyids, particularly H. pumilio.

Citations

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  • Is it possible to eliminate or eradicate human fish-borne parasitic diseases? A sweet dream or a nightmare?
    Jorge C. Eiras
    Current Research in Parasitology & Vector-Borne Diseases.2024; 6: 100203.     CrossRef
  • Global prevalence of 4 neglected foodborne trematodes targeted for control by WHO: A scoping review to highlight the gaps
    Rachel Tidman, Kaushi S. T. Kanankege, Mathieu Bangert, Bernadette Abela-Ridder, Paul R. Torgerson
    PLOS Neglected Tropical Diseases.2023; 17(3): e0011073.     CrossRef
  • Incidence of fish-borne trematode infections and associated factors: results from a cohort study in highly endemic communities in northern Vietnam
    Thao Thi Bich Nguyen, Veronique Dermauw, Dung Thi Bui, Hafid Dahma, Dung Thuy Le, Hien Thi Thu Nguyen, Dung Trung Do, Pierre Dorny, Bertrand Losson, Olivier Vandenberg
    Parasitology Research.2023; 122(6): 1415.     CrossRef
  • Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes
    Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth
    Aquaculture.2022; 548: 737660.     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • Prevalence and Associated Risk Factors of Human Intestinal Helminths Parasitic Infections in Ethiopia: A Systematic Review and Meta-Analysis
    Minichil Liyih, Destaw Damtie, Dires Tegen, Ahmad Mansour
    The Scientific World Journal.2022; 2022: 1.     CrossRef
  • Haplorchis pumilio
    Hudson Pinto
    CABI Compendium.2022;[Epub]     CrossRef
  • Detection of Trematodes from the Host Exotic Aquatic Snail Melanoides tuberculata in an Urban Stormwater System
    Jason M. Post, Rachael J. Reasch, Emily S. Bailey
    Zoonotic Diseases.2022; 2(4): 258.     CrossRef
  • Freshwater as a Sustainable Resource and Generator of Secondary Resources in the 21st Century: Stressors, Threats, Risks, Management and Protection Strategies, and Conservation Approaches
    Doru Bănăduc, Vladica Simić, Kevin Cianfaglione, Sophia Barinova, Sergey Afanasyev, Ahmet Öktener, Grant McCall, Snežana Simić, Angela Curtean-Bănăduc
    International Journal of Environmental Research and Public Health.2022; 19(24): 16570.     CrossRef
  • Current status of human liver fluke infections in the Greater Mekong Subregion
    Banchob Sripa, Apiporn T. Suwannatrai, Somphou Sayasone, Dung Trung Do, Virak Khieu, Yichao Yang
    Acta Tropica.2021; 224: 106133.     CrossRef
  • Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution
    Jong-Yil Chai, Bong-Kwang Jung
    Acta Tropica.2020; 201: 105210.     CrossRef
  • Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR
    Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2020; 58(1): 67.     CrossRef
  • Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism
    Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli
    Animals.2020; 10(3): 456.     CrossRef
  • Haplorchis pumilio (Trematoda: Heterophyidae) as a new fish-borne zoonotic agent transmitted by Melanoides tuberculata (Mollusca: Thiaridae) in Brazil: A morphological and molecular study
    André S. Lopes, Eduardo A. Pulido-Murillo, Alan L. Melo, Hudson A. Pinto
    Infection, Genetics and Evolution.2020; 85: 104495.     CrossRef
  • Prevalence and factors associated with mercury exposure in riverside communities in the Brazilian Western Amazon
    Vanessa Alves Mendes, Débora Aparecida da Silva Santos, Edson dos Santos Farias, Dario Pires de Carvalho, Wanderley Rodrigues Bastos
    Revista Brasileira de Enfermagem.2020;[Epub]     CrossRef
  • Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
    Anh Kieu Thi Tran, Hoa Thuy Doan, Anh Ngoc Do, Van Thi Nguyen, Su Xuan Hoang, Huong Thu Thi Le, Hoa Thi Hoang, Nam Hoang Le, Quyen Bao Thi Le, Tran-Anh Le
    BioMed Research International.2019; 2019: 1.     CrossRef
  • Infection status with zoonotic trematode metacercariae in fish from Lao PDR
    Jong-Yil Chai, Soon-Hyung Lee, Han-Jong Rim, Woon-Mok Sohn, Bounlay Phommasack
    Acta Tropica.2019; 199: 105100.     CrossRef
  • Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR
    Jong-Yil Chai, Taehee Chang, Bong-Kwang Jung, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2019; 57(4): 451.     CrossRef
  • Ultrasonographic investigation of cholangiocarcinoma in Lao PDR
    Ju Yeong Kim, Tai-Soon Yong, Han-Jong Rim, Jong-Yil Chai, Duk-Young Min, Keeseon S. Eom, Woon-Mok Sohn, Jae Hoon Lim, Dongil Choi, Sithat Insisiengmay, Bounlay Phommasack, Bounnaloth Insisiengmay
    Acta Tropica.2018; 182: 128.     CrossRef
  • Echinostoma ilocanum Infection in Two Residents of Savannakhet Province, Lao PDR
    Jong-Yil Chai, Woon-Mok Sohn, Jaeeun Cho, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Han-Jong Rim
    The Korean Journal of Parasitology.2018; 56(1): 75.     CrossRef
  • Fishborne zoonotic heterophyid infections: An update
    Jong-Yil Chai, Bong-Kwang Jung
    Food and Waterborne Parasitology.2017; 8-9: 33.     CrossRef
  • 10,781 View
  • 122 Download
  • 20 Web of Science
  • Crossref

Brief Communication

High Prevalence of Haplorchiasis in Nan and Lampang Provinces, Thailand, Proven by Adult Worm Recovery from Suspected Opisthorchiasis Cases
Adulsak Wijit, Nimit Morakote, Jaewwaew Klinchid
Korean J Parasitol 2013;51(6):767-769.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.767

Opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in Thailand. The Annual Surveillance Reports from Nan and Lampang Provinces, Thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. This study investigated whether most cases are actually due to minute intestinal flukes (MIF) rather than Opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. Fifty and 100 cases from residents in Nan and Lampang, respectively, had stools positive for eggs initially assumed to be those of O. viverrini. Each patient was given praziquantel at 40 mg/kg in a single dose. After 2 hr, 30-45 ml of the purgative magnesium sulfate was given, and stools were collected up to 4 times sequentially. The stools were examined for adult worms by simple sedimentation. It was found that 39 of 50 cases (78.0%) from Nan Province had Haplorchis taichui, with intensities ranging from 5 to 1,250 with an average of 62 worms/case. Taenia saginata (7 cases) and Enterobius vermicularis (1 case) were other helminths recovered as the co-infectants. In Lampang Province, H. taichui was recovered from 69 cases (69.0%). The number of flukes recovered ranged from 1 to 4,277, with an average of 326 worms/case. Four cases had Phaneropsolus bonnei, and 10 T. saginata as the co-infectants. Adult specimens of O. viverrini were not recovered from any stool. Clearly, MIF infection, especially haplorchiasis, is more common in northern Thailand. These findings should encourage the Public Health Office to employ more specific tools than Kato's method for surveillance of opisthorchiasis in Thailand.

Citations

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  • Prevalence and Epidemiological Patterns of Enterobius vermicularis Infection in Thailand: A Systematic Review and Meta-Analysis
    Jurairat Jongthawin, Aongart Mahittikorn, Apiporn Thinkhamrop Suwannatrai, Chutima Rattanawan, Kinley Wangdi, Frederick Ramirez Masangkay, Manas Kotepui
    Medical Sciences.2025; 13(4): 207.     CrossRef
  • Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes
    Picha Suwannahitatorn, Mathirut Mungthin, Ittisak Subrungruang, Lakhanawan Charoensuk, Nithikoon Aksorn, Saiwasan Buathong, Krystyna Cwiklinski
    PLOS Neglected Tropical Diseases.2024; 18(8): e0012445.     CrossRef
  • Gastrointestinal Helminthic Infection among the Population in Northern Thailand
    Ampas Wisetmora, Oranard Wattanawong, Adulsak Wijit, Jutikarn Phukowluan, Ampol Nachairan, Prueksarawuth Jaksuay, Sivapong Sungpradit, Nuttapon Ekobol, Thidarut Boonmars, Alisa Boonsuya, Phornphitcha Pechdee, Chutharat Thanchonnang, Nav La, Nathkapach K.
    Acta Parasitologica.2024; 69(3): 1648.     CrossRef
  • Model-based spatial-temporal mapping of opisthorchiasis in endemic countries of Southeast Asia
    Ting-Ting Zhao, Yi-Jing Feng, Pham Ngoc Doanh, Somphou Sayasone, Virak Khieu, Choosak Nithikathkul, Men-Bao Qian, Yuan-Tao Hao, Ying-Si Lai
    eLife.2021;[Epub]     CrossRef
  • Adenosine Suppresses Cholangiocarcinoma Cell Growth and Invasion in Equilibrative Nucleoside Transporters-Dependent Pathway
    Kornkamon Lertsuwan, Supathra Phoaubon, Nathapol Tasnawijitwong, Jomnarong Lertsuwan
    International Journal of Molecular Sciences.2020; 21(3): 814.     CrossRef
  • Intestinal parasites in rural communities in Nan Province, Thailand: changes in bacterial gut microbiota associated with minute intestinal fluke infection
    Ajala Prommi, Pinidphon Prombutara, Dorn Watthanakulpanich, Poom Adisakwattana, Teera Kusolsuk, Tippayarat Yoonuan, Akkarin Poodeepiyasawat, Nirundorn Homsuwan, Samreong Prummongkol, Malee Tanita, Sungkhom Rattanapikul, Chuanphot Thinphovong, Anamika Krit
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  • Genetic Differentiation of Opisthorchis-Like Eggs in Northern Thailand Using Stool Specimens Under National Strategic Plan to Control Liver Fluke Infection and Cholangiocarcinoma
    Saiwasan Buathong, Kateitsaray Phaiphilai, Toon Ruang-areerate, Naruemon Sitthichot, Thanyapit Thita, Mathirut Mungthin, Picha Suwannahitatorn
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    K. Chaisiri, C. Jollivet, P. Della Rossa, S. Sanguankiat, D. Wattanakulpanich, C. Lajaunie, A. Binot, M. Tanita, S. Rattanapikul, D. Sutdan, S. Morand, A. Ribas
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    Jong-Yil Chai, Bong-Kwang Jung
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    Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-ariya, Edoardo Pozio
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  • 94 Download
  • Crossref

Original Articles

Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
Dongmin Lee, Seongjun Choe, Hansol Park, Hyeong-Kyu Jeon, Jong-Yil Chai, Woon-Mok Sohn, Tai-Soon Yong, Duk-Young Min, Han-Jong Rim, Keeseon S. Eom
Korean J Parasitol 2013;51(6):719-726.
Published online December 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.6.719

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

Citations

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Hyperendemicity of Haplorchis taichui Infection among Riparian People in Saravane and Champasak Province, Lao PDR
Jong-Yil Chai, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Hyeong-Kyu Jeon, Tae-Yun Kim, Bong-Kwang Jung, Lay Sisabath, Bounnaloth Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2013;51(3):305-311.
Published online June 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.3.305

In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 78.8% and 66.4% in Saravane and Champasak province, respectively. The positive rate for small trematode eggs (STE), which included H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 69.9% and 46.3% in Saravane and Champasak province, respectively. To obtain adult flukes, 30 STE-positive people were treated with 40 mg/kg praziquantel and then purged. Whole diarrheic stools were collected 4-5 times for each person and searched for fluke specimens using a stereomicroscope. Mixed infections with various species of trematodes (H. taichui, Haplorchis pumilio, O. viverrini, Prosthodendrium molenkampi, Centrocestus formosanus, and Echinochasmus japonicus) and a species of cestode (Taenia saginata) were found. However, the worm load was exceptionally high for H. taichui compared with other trematode species, with an average of 21,565 and 12,079 specimens per infected person in Saravane and Champasak province, respectively, followed by H. pumilio (41.9 and 22.5, respectively) and O. viverrini (9.4 and 1.5, respectively). These results show that diverse species of intestinal and liver flukes are prevalent among riparian people in Saravane and Champasak province, Lao PDR, with H. taichui being the exceptionally dominant species.

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Fishborne Trematode Metacercariae in Luang Prabang, Khammouane, and Saravane Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisiengmay
Korean J Parasitol 2013;51(1):107-114.
Published online February 18, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.1.107

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.

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Brief Communication

Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
Hyeong-Kyu Jeon, Dongmin Lee, Hansol Park, Duk-Young Min, Han-Jong Rim, Hongman Zhang, Yichao Yang, Xueming Li, Keeseon S Eom
Korean J Parasitol 2012;50(4):391-394.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.391

The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.

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Original Article

Foodborne Trematode Metacercariae in Fish from Northern Vietnam and Their Adults Recovered from Experimental Hamsters
Jong-Yil Chai, Nguyen Van De, Woon-Mok Sohn
Korean J Parasitol 2012;50(4):317-325.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.317

The prevalence of foodborne trematode (FBT) metacercariae was investigated in fish from 2 localities of northern Vietnam in 2004-2005. Freshwater fish (9 species) were collected from local markets in Hanoi City (n=76) and Nam Dinh Province (n=79), and were examined for FBT metacercariae using the artificial digestion technique. Adult flukes were obtained from hamsters experimentally infected with the metacercariae at day 8 post-infection. Three (Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium) and 6 (Haplorchis taichui, H. pumilio, C. formosanus, P. varium, Stellantchasmus falcatus, and Heterophyopsis continua) species of FBT metacercariae were detected in the 2 regions, respectively. Overall, among the positive fish species, H. pumilio metacercariae were detected in 104 (80.0%) of 130 fish examined (metacercarial density per infected fish; 64.2). C. formosanus metacercariae were found in 37 (40.2%) of 92 fish (metacercarial density; 14.7). P. varium metacercariae were detected in 19 (63.3%) of 30 fish (Anabas testudineus and Mugil cephalus) (metacercarial density; 247.7). S. falcatus metacercariae were found in all 10 M. cephalus examined (metacercarial density; 84.4). H. continua metacercariae (2 in number) were detected in 1 fish of Coilia lindmani. Morphologic characteristics of the FBT metacercariae and their experimentally obtained adults were described. The results have demonstrated that various FBT species are prevalent in northen parts of Vietnam.

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Brief Communications

Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
Thapana Chontananarth, Chalobol Wongsawad
Korean J Parasitol 2010;48(4):343-346.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.343

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.

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Prevalence of the Intestinal Flukes Haplorchis taichui and H. yokogawai in a Mountainous Area of Phongsaly Province, Lao PDR
Jong-Yil Chai, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Eun-Hee Shin, Virasack Banouvong, Bounnaloth Insisiengmay, Sithat Insisiengmay, Bounlay Phommasack, Han-Jong Rim
Korean J Parasitol 2010;48(4):339-342.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.339

Phongsaly Province, located in the northernmost area of Lao PDR, was previously suggested to be endemic for the liver fluke Opisthorchis viverrini infection. To confirm, or rule out, this suggestion, the Phonxay village in the Khoua District, Phongsaly Province, was selected for a survey. Ten volunteers (8 men and 2 women aged 31-57 years) who consumed raw freshwater fish and had gastrointestinal troubles were treated with a single dose of praziquantel (40 mg/kg) and pyrantel pamoate (10 mg/kg) and purged with magnesium sulfate to recover any worm parasites. Eight of the 10 volunteers expelled 1 or more species of trematodes, nematodes, or cestodes (worm positive rate; 80%). The worms were morphologically identified as H. taichui (861 worms from 8 people), H. yokogawai (59 from 6 people), Phaneropsolus bonnei (1 from 1 person), Trichostrongylus sp. (2 from 2 people), Ascaris lumbricoides (2 from 1 person), Enterobius vermicularis (11 from 3 people), and Taenia saginata (1 strobila with scolex from 1 person). The results indicate that the mountainous area of Phongsaly Province, Lao PDR, is not endemic for the liver fluke but endemic for intestinal flukes, in particular, Haplorchis taichui and H. yokogawai.

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Original Article

Haplorchis taichui as a Possible Etiologic Agent of Irritable Bowel Syndrome-Like Symptoms
Dorn Watthanakulpanich, Jitra Waikagul, Wanna Maipanich, Supaporn Nuamtanong, Surapol Sanguankiat, Somchit Pubampen, Rangson Praevanit, Srisuchat Mongkhonmu, Yukifumi Nawa
Korean J Parasitol 2010;48(3):225-229.
Published online September 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.3.225

The aim of this study is to clarify the clinical features of Haplorchis taichui infection in humans in Nan Province, Thailand, and to correlate the clinical features with irritable bowel syndrome (IBS)-like symptoms. In this study area, only H. taichui, but neither other minute intestinal flukes nor small liver flukes were endemic. The degree of infection was determined by fecal egg counts and also by collecting adult worms after deworming. The signs and symptoms of individual patients together with their hematological and biochemical laboratory data were gathered to evaluate the relationship between the clinical features and the severity of infection. Special emphasis was made to elucidate the possible similarities of the clinical features of H. taichui infection and IBS-like symptoms. The results showed useful clinical information and the significant (> 50%) proportion of haplorchiasis patients complained of abdominal pain, lassitude, and flatulence, which were the important diagnostic symptoms of IBS. This study has reported a possible link between H. taichui and IBS, and H. taichui might probably play a role in the etiology of these IBS-like symptoms.

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Brief Communication

Egg Laying Capacity of Haplorchis taichui (Digenea: Heterophyidae) in Humans
Megumi Sato, Surapol Sanguankiat, Somchit Pubampen, Teera Kusolsuk, Wanna Maipanich, Jitra Waikagul
Korean J Parasitol 2009;47(3):315-318.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.315

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.

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Original Articles

Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China
Woon-Mok Sohn, Keeseon S. Eom, Duk-Young Min, Han-Jong Rim, Eui-Hyug Hoang, Yichao Yang, Xueming Li
Korean J Parasitol 2009;47(3):249-257.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.249

A survey was performed to investigate the infection status of fishborne trematode (FBT) metacercariae in freshwater fish from Guangxi Zhuang Autonomous Region, China. A total of 307 freshwater fish of 31 species were collected from 5 administrative regions of Guangxi Zhuang Autonomous Region. They were examined by artificial digestion method from July 2003 to August 2004. No metacercariae were detected in fish from Fusui-xian. In fish from Mashan-xian and a market in Nanning, 3 species of metacercariae, Haplorchis taichui, Haplorchis pumilio, and Centrocestus formosanus, were mainly detected. Metacercariae (8 in number) of Clonorchis sinensis were found in 1 Chanodichthys dabryi purchased from a market in Nanning. In fish from Yangshuo, Metagonimus yokogawai metacercariae were detected from all 18 fish species examined. Total 13 C. sinensis metacercariae were found in 3 out of 10 Hemibarbus maculatus from Yangshuo. All 7 Zacco platypus from Yangshuo were infected with 8-112 Echinochasmus perfoliatus metacercariae. In fish from Binyang-xian, H. pumilo metacercariae were mainly detected in all 5 fish species examined, and only 1 metacercaria of C. sinensis was found in a Hemiculter leucisculus. From the above results, it was confirmed that some species of freshwater fish play a role of second intermediate hosts for FBT in Guangxi Zhuang Autonomous Region, China. In particular, 4 species of intestinal flukes, M. yokogawai, H. taichui, H. pumilio, and C. formosanus, were prevalent in fish hosts, whereas C. sinensis metacercariae were detected only in 3 fish species.

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High Prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and Other Helminth Infections among People in Khammouane Province, Lao PDR
Jong-Yil Chai, Eun-Taek Han, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Jin-Young Um, Min-Sung Park, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2009;47(3):243-247.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.243

The prevalence of liver and intestinal helminth infections, including Opisthorchis, Haplorchis, Phaneropsolus, hookworms, Enterobius, and Taenia, was surveyed in Khammouane province, Lao PDR. Fecal specimens were collected from 1,242 people (590 men and 652 women) in 3 Mekong riverside villages and were examined by the Kato-Katz thick smear technique. The overall helminth egg positive rate was 81.1%. The positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, and lecithodendriids, was 81.1% and the positive rate for hookworms was 6.7%. To obtain adult worms, 35 people who were positive for small trematode eggs were treated with 20-30 mg/kg praziquantel and 10-15 mg/kg pyrantel pamoate, and then purged. Diarrheic stools were collected from 33 of these people and searched for helminth parasites using a stereomicroscope. Mixed infections with various helminths (Haplorchis taichui, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, echinostomes, hookworms, Trichostrongylus spp., Trichuris trichiura, Enterobius vermicularis, and/or Taenia saginata) were found. The total number of helminth specimens collected was 20,907 (approximately 634 per person). The most common species was H. taichui, followed by P. molenkampi, O. viverrini, P. bonnei, E. vermicularis, hookworms, and Trichostrongylus spp. These results show that diverse species of intestinal nematodes, trematodes, and cestodes are infecting humans in Khammouane province, Lao PDR.

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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Han-Jong Rim, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Jong-Yil Chai, Duk-Young Min, Soon-Hyung Lee, Eui-Hyug Hoang, Bounlay Phommasack, Sithat Insisengmay
Korean J Parasitol 2008;46(4):253-260.
Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.253

Freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR were examined by the muscle compression and artificial digestion methods to know the infection status with trematode metacercariae. In the fish from Savannakhet, 2 species of metacercariae, Opisthorchis viverrini and Haplorchis taichui, were detected. O. viverrini metacercariae were found in 6 species of fish, Puntius brevis, Hampala dispar, Esomus metallicus, Mystacoleucus marginatus, Puntioplites falcifer, and Cyclocheilichthys armatus. H. taichui metacercariae were detected in 3 species of fish, P. brevis, P. falcifer, and M. marginatus. In the fish from Vientiane, 4 species of metacercariae, O. viverrini, H. taichui, Haplorchis yokogawai, and Centrocestus formosanus, were detected. Among them, O. viverrini metacercariae were found in 7 species of fish, Onychostoma elongatum, C. armatus, H. dispar, P. brevis, Cyclocheilichthys repasson, Osteochilus hasseltii, and Hypsibarbus lagleri. The metacercariae of H. taichui were detected in 6 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, and Cirrhinus molitorella. The metacercariae of H. yokogawai were found in 9 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, O. hasseltii. C. molitorella, Hypsibarbus wetmorei, and H. lagleri. The metacercariae of C. formosanus were detected in 4 species of fish, C. repasson, P. brevis, O. hasseltii, and C. molitorella. From these results, it is confirmed that fishborne trematode metacercariae, i.e. O. viverrini, H. taichui, H. yokogawai and C. formosanus, are prevalent in various species of freshwater fish from Savannakhet Province and Vientiane Municipality, Lao PDR.

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Brief Communication

Prevalence of Haplorchis taichui and Haplorchoides sp. Metacercariae in Freshwater Fish from Water Reservoirs, Chiang Mai, Thailand
Choosak Nithikathkul, Chalobol Wongsawad
Korean J Parasitol 2008;46(2):109-112.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.109

A parasitological investigation on trematode metacercariae was made on 62 freshwater fishes of 13 species in northern Thailand; Cyclocheilichthys apogon, Puntioplites proctozysron, Labiobarbus siamensis, Barbodes gonionotus, Barbodes altus, Henicorhynchus siamensis, Osteochilus hasselti, Notopterus notopterus, Mystacoleucus marginatus, Anabas testudineus, Systomus orphoides, Morulius chrysophykadian, and Hampala macrolepidota. The fish were caught over the summer period (February-May 2007) from 2 Chiang Mai water reservoirs, i.e., the Mae Ngad (UTM 47Q E 503200, 47Q N 2119300) and the Mae Kuang Udomtara (UTM 47Q E 513000, 47Q N 2092600) Reservoirs in Chiang Mai province, Thailand. The prevalence of heterophyid (Haplorchis taichui and Haplorchoides sp.) metacercariae in these fish was 83.9% and 74.2% in the Mae Ngad and Mae Kuang Udomtara Reservoirs, respectively. The highest intensity of heterophyid metacercariae in H. siamensis in the Mae Ngad was 120.4 and that in P. proctozysron in the Mae Kuang Udomtara was 180.0. The fish, A. testudineus, C. apogon, and M. chrysophykadian, were not found to be infected with H. taichui metacercariae. The results show that the freshwater fish in Chiang Mai water reservoirs are heavily infected with H. taichui and Haplorchoides sp. metacercariae.

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Original Article
High prevalence of liver and intestinal fluke infections among residents of Savannakhet Province in Laos
Jong-Yil Chai, Eun-Taek Han, Sang-Mee Guk, Eun-Hee Shin, Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Keon-Hoon Lee, Hoo-Gn Jeong, Yong-Sang Ryang, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Soon-Hyung Lee, Han-Jong Rim
Korean J Parasitol 2007;45(3):213-218.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.213

The prevalence of liver and intestinal fluke infections was surveyed on residents of Savannakhet Province, Laos. Fecal specimens were collected from a total of 981 residents in 4 Mekong riverside villages and examined by the Kato-Katz thick smear technique. The results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including Opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. To obtain adult flukes, 38 small trematode egg positive cases were treated with a 20-30 mg/kg single dose of praziquantel and purged. Diarrheic stools were then collected from 29 people and searched for helminth parasites using stereomicroscopes. Mixed infections with O. viverrini and 6 kinds of intestinal flukes were found, namely, Haplorchis taichui, Haplorchis pumilio, Haplorchis yokogawai, Prosthodendrium molenkampi, Phaneropsolus bonnei, and echinostomes. The total number of flukes collected was 7,693 specimens (av. no. per treated person; 265.3). The most common species was O. viverrini, followed by H. taichui, P. molenkampi, echinostomes, H. pumilio, P. bonnei, and H. yokogawai. The results indicate that foodborne liver and intestinal fluke infections are prevalent among residents of Savannakhet Province, Laos.

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