Parasites, Hosts and Diseases

"Hong Yin"

Original Articles

Molecular Characterization of Hard Ticks by Cytochrome c Oxidase Subunit 1 Sequences: Huitian Gou, Huiwen Xue, Hong Yin, Jianxun Luo, Xiaolin Sun; Korean J Parasitol 2018;56(6):583-588.
Published online December 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.6.583

Although widely studied, the natural diversity of the hard tick is not well known. In this study, we collected 194 sequences from 67 species, covering 7 genera of hard tick. The 5’ region of the mitochondrial cytochrome c oxidase subunit 1 region (586 bp) has been used to investigate intra- and inter-species variation and the phylogenetic tree of neighbor joining method has been used for assessment. As a result, by comparing the K2P-distance of intra- and interspecies, 30 samples (15.2%) shown that interspecies distance was larger than the minimum interspecfic distance. From the phylogenetic analysis, 86.8% (49) of the species were identified correctly at the genus level. On deeper analysis on these species suggested the possibility of presence cryptic species. Therefore, further work is required to delineate species boundaries and to develop a more complete understanding of hard tick diversity over larger scale.

Citations

Citations to this article as recorded by

First European record of Rickettsia bellii in Amblyomma rotundatum from Rhinella marina imported to Poland
Dagmara Dyczko, Dominik M. Szymański, Dawid Szymański, Maciej Kupczak, Krzysztof Kolenda
Experimental and Applied Acarology.2025;[Epub] CrossRef
Ticks on migrating birds in southwestern Poland: occurrence of Ixodes ricinus and the first Polish record of Haemaphysalis concinna on birds
Dagmara Dyczko, Lucyna Hałupka, Beata Czyż, Aleksandra Czułowska, Dorota Kiewra
Experimental and Applied Acarology.2025;[Epub] CrossRef
Candidatus Midichloria mitochondrii can be vertically transmitted in Hyalomma anatolicum
Tingxiang Luo, Ercha Hu, Lu Gan, Depeng Yang, Jun Wu, Shenghong Gao, Xiaoli Tuo, chahan Gailike Bayin, Zhengxiang Hu, Qingyong Guo
Experimental Parasitology.2024; 265: 108828. CrossRef
Molecular characterization of Hyalomma scupense and its vector-borne pathogen Theileria annulata in Ksar El Boukhari (Medea, Algeria)
Naila Aouali, Asmaa Sekkai, Thinhinane Djouaher, Zahra Messaoudi, Hocine Ziam, Amina Boutellis, Tahar Kernif
Comparative Immunology, Microbiology and Infectious Diseases.2024; 114: 102243. CrossRef
Seasonal dynamics of Haemaphysalis tick species as SFTSV vectors in South Korea
Hyunwoo Jang, Mark Anthony B. Casel, Seung-gyu Jang, Jeong Ho Choi, Juryeon Gil, Rare Rollon, So youn Cheun, Young-Il Kim, Min Suk Song, Young Ki Choi, Anna Moniuszko-Malinowska
Microbiology Spectrum.2024;[Epub] CrossRef
Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
Fevzi Bardakci, Sarah Hilan Mohammed Al-Subaie, Riadh Badraoui, Mohd Adnan, Arif Jamal Siddiqui
Life.2023; 13(7): 1535. CrossRef
Distribution and molecular identification of ixodid ticks infesting cattle in Kilombero and Iringa Districts, Tanzania
Walter S Magesa, Isihaka Haji, Edson Kinimi, Jahashi S Nzalawahe, Rudovick Kazwala
BMC Veterinary Research.2023;[Epub] CrossRef
Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in inner Mongolia, China
Yunyi Kong, Chao Yan, Dongxiao Liu, Lingling Jiang, Gang Zhang, Biao He, Yong Li
Ticks and Tick-borne Diseases.2022; 13(1): 101856. CrossRef
DNA barcoding of hard ticks (Ixodidae), notes on distribution of vector species and new faunal record for Croatia
Stjepan Krčmar, Ana Klobučar, Marko Vucelja, Marko Boljfetić, Mladen Kučinić, Josip Madić, Maja Cvek, Branka Bruvo Mađarić
Ticks and Tick-borne Diseases.2022; 13(3): 101920. CrossRef
Ticks (Acari: Ixodidae) and Associated Pathogens Collected From Domestic Animals and Vegetation in Stann Creek District, Southeastern Belize, Central America
Suppaluck Polsomboon Nelson, Brian P Bourke, Razan Badr, John Tarpey, Laura Caicedo-Quiroga, Donovan Leiva, Marie Pott, Alvaro Cruz, Chien-Chung Chao, Nicole L Achee, John P Grieco, Le Jiang, Ju Jiang, Christina M Farris, Yvonne-Marie Linton, Kevin Macalu
Journal of Medical Entomology.2022; 59(5): 1749. CrossRef
Metatranscriptomics Reveals the Diversity of the Tick Virome in Northwest China
Yunyi Kong, Gang Zhang, Lingling Jiang, Pu Wang, Sinong Zhang, Xiaomin Zheng, Yong Li, Biao He
Microbiology Spectrum.2022;[Epub] CrossRef
Descriptive DNA barcoding of Argas (Persicargas) arboreus and Argas (Persicargas) persicus ticks (Ixodida: Argasidae) infesting birds in Egypt
Enas H. Ghallab, Ayat Yousery, Mona G. Shaalan
Experimental and Applied Acarology.2022; 88(3-4): 397. CrossRef
Tick species identification and molecular detection of tick-borne pathogens in blood and ticks collected from cattle in Egypt
Amira AL-Hosary, Cristian Răileanu, Oliver Tauchmann, Susanne Fischer, Ard M. Nijhof, Cornelia Silaghi
Ticks and Tick-borne Diseases.2021; 12(3): 101676. CrossRef
Detection of novel mitochondrial mutations in cytochrome C oxidase subunit 1 (COX1) in patients with familial adenomatous polyposis (FAP)
E. Afkhami, M. M. Heidari, M. Khatami, F. Ghadamyari, S. Dianatpour
Clinical and Translational Oncology.2020; 22(6): 908. CrossRef

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Biological Parameters of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) Fed on Rabbits, Sheep, and Cattle: Miling Ma, Ze Chen, Aihong Liu, Qiaoyun Ren, Junlong Liu, Zhijie Liu, Youquan Li, Hong Yin, Guiquan Guan, Jianxun Luo; Korean J Parasitol 2016;54(3):301-305.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.301

Abstract
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In order to determine the effect of various hosts on feeding performance of Rhipicephalus (Boophilus) microplus, we used 3 mammalian species as hosts, cattle (Qinchuan), sheep (T an), and rabbits (Japanese white rabbit) for infest-ing ticks. Five hundreds of R. microplus larvae were exposed to each animal (3 animals/host species). Tick recoveries were 11.0%, 0.47%, and 5.5% from cattle, sheep, and rabbits, respectively. The averages of tick feeding periods were not significantly different on cattle, sheep, and rabbits, 28.8, 25.3, and 26.7 days, respectively. The average weights of individual engorged female from cattle, sheep, and rabbits were 312.5, 219.1, and 130.2 mg, respectively and those of egg mass weights each to 85.0, 96.6, and 17.8 mg. The highest egg hatching rate was in the ticks from cattle (96.0%), fol-lowed by those from rabbits (83.0%) and sheep (19.2%). These data suggest that rabbits could be as an alternative host to cultivate R. microplus for evaluating vaccines and chemical and biological medicines against the tick in the laboratory, although the biological parameters of ticks were less than those from cattle.

Citations

Citations to this article as recorded by

Variation in the hatching rate of larvae of four tick species from laboratory colonies
Ygor Henrique da Silva, Marisa Beatriz da Silva Rocha, Ester Oliozi Marré, Manuela Pimentel da Motta, Brena Gava Guimarães, Thais Ribeiro Correia Azevedo, Guilherme Marcondes Klafke, Barbara Rauta de Avelar, Diefrey Ribeiro Campos, Fabio Barbour Scott
Revista Brasileira de Parasitologia Veterinária.2025;[Epub] CrossRef
Adaptation and Invasion Dynamics of Rhipicephalus microplus in South Africa: Ecology, Resistance, and Management Implications
Tsireledzo Goodwill Makwarela, Nimmi Seoraj-Pillai, Dikeledi Petunia Malatji, Tshifhiwa Constance Nangammbi
Insects.2025; 16(12): 1204. CrossRef
The effect of feeding on different hosts on the egg proteins in Haemaphysalis qinghaiensis tick
Yong Li, Rong Cheng, Xiao-Yu Liu, Darko Mihaljica, Tian-Yin Cheng
Parasitology Research.2024;[Epub] CrossRef
Aurora kinase as a putative target to tick control
Bruno Moraes, Helga Gomes, Luiz Saramago, Valdir Braz, Luís Fernando Parizi, Gloria Braz, Itabajara da Silva Vaz, Carlos Logullo, Jorge Moraes
Parasitology.2024; 151(9): 983. CrossRef
Genetic diversity of tick (Acari: Ixodidae) populations and molecular detection of Anaplasma and Ehrlichia infesting beef cattle from upper-northeastern Thailand
Kanchana Thinnabut, Rutchanee Rodpai, Oranuch Sanpool, Wanchai Maleewong, Ubon Tangkawanit
Infection, Genetics and Evolution.2023; 107: 105394. CrossRef
Infestation of rabbits with just-molted adults of the cattle tick Rhipicephalus microplus: biological parameters and efficiency
Milagros Vargas-Hernandez, Carlos Montero-Espinosa, Dunia Sánchez-Villaurrutia, Carlos Antonio Duarte, Gervasio Henrique Bechara, Alier Fuentes-Castillo, Julio Ancisar, José Suárez-Alba, Omar Mosqueda-Lobaina, Marisela Suárez-Pedroso
Revista Brasileira de Parasitologia Veterinária.2023;[Epub] CrossRef
Synergistic attraction of Western black-legged ticks, Ixodes pacificus , to CO 2 and odorant emissions from deer-associated microbes
Justin Long, Keiran Maskell, Regine Gries, Saif Nayani, Claire Gooding, Gerhard Gries
Royal Society Open Science.2023;[Epub] CrossRef
Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario
Arlex Rodríguez-Durán, Shafi Ullah, Luís Fernando Parizi, Abid Ali, Itabajara da Silva Vaz Junior
Pathogens.2023; 12(9): 1117. CrossRef
Molecular detection of Rickettsia amblyommatis and Rickettsia parkeri in ticks collected from wild pigs in Campeche, Mexico
Andrés M. López-Pérez, Sokani Sánchez-Montes, Brenda Aline Maya-Badillo, Guillermo Orta-Pineda, Saúl Reveles-Félix, Ingeborg Becker, Karla Bárcenas-Barreto, Adán Torres-Monroy, Rafael Ojeda-Flores, José Iván Sánchez-Betancourt
Ticks and Tick-borne Diseases.2022; 13(1): 101844. CrossRef
Identification of Rickettsia spp., Anaplasma spp., and an Ehrlichia canis-like agent in Rhipicephalus microplus from Southwest and South-Central China
Miao Lu, Junhua Tian, Xingliang Pan, Xincheng Qin, Wen Wang, Jintao Chen, Wenping Guo, Kun Li
Ticks and Tick-borne Diseases.2022; 13(2): 101884. CrossRef
Spatial distribution patterns of tick community structure in sympatric jaguars (Panthera onca) and pumas (Puma concolor) from three ecoregions in Mexico
Andrés M. López‐Pérez, Octavio López‐Fernández, Laura Backus, Scott Somerville, Heliot Zarza, Ivonne Cassaigne, Antonio de la Torre, Rodrigo Nuñez‐Perez, Gerardo Ceballos, Carmen Guzmán‐Cornejo, Susana Ringenbach‐Valdez, Miguel A. del Rio, Sokani Sánchez‐
Medical and Veterinary Entomology.2022; 36(3): 371. CrossRef
Rhipicephalus annulatus, R. australis or R. microplus? Discordance between morphological and genetic data among three cattle tick species
A. R. Kazim, V. L. Low, D. Tappe, J. Houssaini, C. C. Heo
Experimental and Applied Acarology.2022; 87(1): 119. CrossRef
First digital characterization of the transhumance corridors through Benin used by cattle herds from Burkina Faso and associated risk scoring regarding the invasion of Rhipicephalus ( Boophilus
Olivier M. Zannou, Achille S. Ouedraogo, Abel S. Biguezoton, Laetitia Lempereur, Kouassi Patrick Yao, Emmanuel Abatih, Sébastien Zoungrana, Marc Lenaert, Patrice Toe, Souaïbou Farougou, Claude Saegerman
Transboundary and Emerging Diseases.2021; 68(4): 2079. CrossRef
Anaplasma marginale in goats from a multispecies grazing system in northeastern Brazil
Iago C. Barbosa, Marcos R. André, Renan Bressianini do Amaral, Jessica D.M. Valente, Priscylla C. Vasconcelos, Celso J.B. Oliveira, Marcia Mariza Gomes Jusi, Rosangela Z. Machado, Thállitha S.W.J. Vieira, Massaro W. Ueti, Rafael F.C. Vieira
Ticks and Tick-borne Diseases.2021; 12(1): 101592. CrossRef
Theileria orientalis outbreak in an organized cattle breeding farm
Vanita Patial, Tania Gupta, Shivani Angaria, Diksha Bali, Ajay Katoch, Mayank Gautam, Nirbhay Kumar Singh, Mandeep Sharma, Rajesh Chahota
Veterinary Parasitology: Regional Studies and Reports.2021; 24: 100572. CrossRef
Characterization of two Cuban colonies of Rhipicephalus microplus ticks
Pedro E. Encinosa Guzmán, Claudia Fernández Cuétara, Ana Laura Cano Argüelles, Alier Fuentes Castillo, Yuselys García Martínez, Rafmary Rodríguez Fernández, Yilian Fernández Afonso, Yami Bello Soto, Yorexis González Alfaro, Luis Méndez, Angelina Díaz Garc
Veterinary Parasitology: Regional Studies and Reports.2021; 25: 100591. CrossRef
Updating the application of Metarhizium anisopliae to control cattle tick Rhipicephalus microplus (Acari: Ixodidae)
Walter O. Beys-da-Silva, Rafael L. Rosa, Markus Berger, Caio J.B. Coutinho-Rodrigues, Marilene H. Vainstein, Augusto Schrank, Vânia R.E. P. Bittencourt, Lucélia Santi
Experimental Parasitology.2020; 208: 107812. CrossRef
Effects of introduced sika deer (Cervus nippon) and population control activity on the distribution of Haemaphysalis ticks in an island environment
Kandai Doi, Katsunori Nishida, Takuya Kato, Shin-ichi Hayama
International Journal for Parasitology: Parasites and Wildlife.2020; 11: 302. CrossRef
Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil
Anna Claudia Baumel Mongruel, Viviane Campos Spanhol, Jessica Damiana Marinho Valente, Petrônio Pinheiro Porto, Liza Ogawa, Flávio Haragushiku Otomura, Ellen de Souza Marquez, Marcos Rogério André, Thállitha Samih Wischral Jayme Vieira, Rafael Felipe da C
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Ticks (Acari: Ixodoidea) in China: Geographical distribution, host diversity, and specificity
Yan‐Kai Zhang, Xiao‐Yu Zhang, Jing‐Ze Liu
Archives of Insect Biochemistry and Physiology.2019;[Epub] CrossRef
Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines
Adrian P. Ybañez, Orgil V. Arrabis, Dennis Justin M. Alvarez, Eloiza May S. Galon, Rhea Mae P. Jayag, Elmie S. Delan, Rochelle Haidee D. Ybañez, Xuenan Xuan
Veterinary World.2019; 12(6): 774. CrossRef
First confirmed report of outbreak of theileriosis/anaplasmosis in a cattle farm in Henan, China
Yanyan Cui, Xiaoxing Wang, Yan Zhang, Yaqun Yan, Haiju Dong, Fuchun Jian, Ke Shi, Longxian Zhang, Rongjun Wang, Changshen Ning
Acta Tropica.2018; 177: 207. CrossRef
First report of Anaplasma marginale infection in goats, Brazil
Nayara B. da Silva, Naomi S. Taus, Wendell C. Johnson, Anabela Mira, Leonhard Schnittger, Jessica D. M. Valente, Odilon Vidotto, Hayley E. Masterson, Thállitha S. W. J. Vieira, Massaro W. Ueti, Rafael F. C. Vieira, Roman R. Ganta
PLOS ONE.2018; 13(8): e0202140. CrossRef

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Validation of Reference Genes for Quantitative Real-Time PCR in Bovine PBMCs Transformed and Non-transformed by Theileria annulata: Hongxi Zhao, Junlong Liu, Youquan Li, Congshan Yang, Shuaiyang Zhao, Juan Liu, Aihong Liu, Guangyuan Liu, Hong Yin, Guiquan Guan, Jianxun Luo; Korean J Parasitol 2016;54(1):39-46.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.39

Abstract
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Theileria annulata is a tick-borne intracellular protozoan parasite that causes tropical theileriosis, a fatal bovine lymphoproliferative disease. The parasite predominantly invades bovine B lymphocytes and macrophages and induces host cell transformation by a mechanism that is not fully comprehended. Analysis of signaling pathways by quantitative real-time PCR (qPCR) could be a highly efficient means to understand this transformation mechanism. However, accurate analysis of qPCR data relies on selection of appropriate reference genes for normalization, yet few papers on T. annulata contain evidence of reference gene validation. We therefore used the geNorm and NormFinder programs to evaluate the stability of 5 candidate reference genes; 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ACTB (β-actin), PRKG1 (protein kinase cGMP-dependent, type I) and TATA box binding protein (TBP). The results showed that 18S rRNA was the reference gene most stably expressed in bovine PBMCs transformed and non-transformed with T. annulata, followed by GAPDH and TBP. While 18S rRNA and GAPDH were the best combination, these 2 genes were chosen as references to study signaling pathways involved in the transformation mechanism of T. annulata.

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A Theileria annulata parasite with a single mutation, methionine 128 to isoleucine (M128I), in cytochrome B is resistant to buparvaquone
Shahin Tajeri, Debasish Chattopadhyay, Gordon Langsley, Ard M. Nijhof, Vikrant Sudan
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Ran Xu, Umesh K. Shandilya, Alexandros Yiannikouris, Niel A. Karrow
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Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
Melânia Lazzari Rigo, Kauê Rodriguez Martins, Yan Wahast Islabão, Alexia Brauner de Mello, Marjorie de Giacometi, Rodrigo Casquero Cunha, Monique Tomazele Rovani, Camila Belmonte Oliveira
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1,25‐Dihydroxyvitamin D3 potentiates the innate immune response of peripheral blood mononuclear cells from Japanese Black cattle
Youki Oyamada, Ei'ichi Iizasa, Amane Usa, Konosuke Otomaru
Animal Science Journal.2023;[Epub] CrossRef
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Ting-Yu Cheng, Jeffrey J. Zimmerman, Luis G. Giménez-Lirola
Animal Health Research Reviews.2022; 23(2): 147. CrossRef
Genetic diversity of Siberian bovine coronavirus isolates (Coronaviridae: Coronavirinae: Betacoronavirus-1: Bovine-Like coronaviruses)
Alexander G. Glotov, Aleksej V. Nefedchenko, Anton G. Yuzhakov, Svetlana V. Koteneva, Tatyana I. Glotova, Alina K. Komina, Nikita Yu. Krasnikov
Problems of Virology.2022; 67(6): 465. CrossRef
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Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Membranes.2021; 11(12): 933. CrossRef
Inhibition monitoring in veterinary molecular testing
Lifang Yan, Kathy L. Toohey-Kurth, Beate M. Crossley, Jianfa Bai, Amy L. Glaser, Rebecca L. Tallmadge, Laura B. Goodman
Journal of Veterinary Diagnostic Investigation.2020; 32(6): 758. CrossRef
Development and testing of the real-time polymerase chain reaction for identification and quantitative determination of the bovine respiratory syncytial virus
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A. V. Nefedchenko, A. G. Glotov, S. V. Koteneva, T. I. Glotova
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Brief Communications

Morphological Characteristics of Normal and Gynandromorphic Hyalomma asiaticum Schulze and Schlottke, 1930: Ze Chen, You-quan Li, Qiao-Yun Ren, Jin Luo, Yonghong Hu, Kai Li, Guang-Yuan Liu, Jian-xun Luo, Jingze Liu, Hong Yin; Korean J Parasitol 2015;53(3):361-364.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.361

Abstract
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Gynandromorphic ticks are extremely rare, and often attract parasitologists’ attention. During our examination of tick specimens, an engorged gynandromorph of Hyalomma asiaticum was noticed. This is the first record of gynandromorphic ticks from China. In this study, several important morphological structures of normal and gynandromorphic H. asiaticum were analyzed. Comparing to the normal H. asiaticum, the gynandromorphic specimen was a typical bipartite protogynander. Its right side showed normal female characteristics, whereas the left side had normal male traits. Different from other gynandromorphic ticks containing 1 anus, this tick reported here had 2 complete anuses, and the anus of the male part had a single adanal plate.

Citations

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A case of gynandromorphism in Hyalomma anatolicum (Ixodida: Ixodidae)
Yan Liu, Xiaoyun Mi, Bingjie Wang, Jun Wu, Wenwen He, Tingxiang Luo, Depeng Yang, Zhengxiang Hu, Lu Gan, Mingdalai Nuo, Huizhen Zheng, Ercha Hu, Qingyong Guo
Experimental and Applied Acarology.2023; 91(1): 133. CrossRef
Morphological abnormalities in Hyalomma dromedarii and Hyalomma rufipes (Acari: Ixodidae) collected from dromedary camels (Camelus dromedarius) in Aswan, Egypt
Mohammed Okely, Deon K. Bakkes, Lidia Chitimia-Dobler
Experimental and Applied Acarology.2022; 88(2): 225. CrossRef
Sequencing of complete mitochondrial genomes confirms synonymization of Hyalomma asiaticum asiaticum and kozlovi, and advances phylogenetic hypotheses for the Ixodidae
Zhi-Qiang Liu, Yan-Feng Liu, Nuer Kuermanali, Deng-Feng Wang, Shi-Jun Chen, Hui-Ling Guo, Li Zhao, Jun-Wei Wang, Tao Han, Yuan-Zhi Wang, Jie Wang, Chen-Feng Shen, Zhuang-Zhi Zhang, Chuang-Fu Chen, Ulrike Gertrud Munderloh
PLOS ONE.2018; 13(5): e0197524. CrossRef
Abnormal Development of Haemaphysalis qinghaiensis (Acari: Ixodidae)
Qiaoyun Ren, Ze Chen, Jin Luo, Guangyuan Liu, Guiquan Guan, Hong Yin, Jianxun Luo
Journal of Insect Science.2016;[Epub] CrossRef

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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR: Yanhua Wang, Guangxiang Wang, Delin Zhang, Hong Yin, Meng Wang; Korean J Parasitol 2013;51(5):573-577.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.573

Abstract
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A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

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Clinical validation of visual LAMP and qLAMP assays for the rapid detection of Toxoplasma gondii
Zhi Cao, Ke Zhang, Dehua Yin, Qiaoya Zhang, Ying Yu, Jianxin Wen, Hongbo Ni
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Mona K. Hegazy, Soha I. Awad, Nora E. Saleh, Mamdouh M. Hegazy
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Mariem Rouatbi, Yosra Amdouni, Safa Amairia, Mohamed R. Rjeibi, Said Sammoudi, Mourad Rekik, Mohamed Gharbi
Veterinary Medicine and Science.2017; 3(1): 22. CrossRef
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Original Articles

Phylogenetic Analysis of Ruminant Theileria spp. from China Based on 28S Ribosomal RNA Gene: Huitian Gou, Guiquan Guan, Miling Ma, Aihong Liu, Zhijie Liu, Zongke Xu, Qiaoyun Ren, Youquan Li, Jifei Yang, Ze Chen, Hong Yin, Jianxun Luo; Korean J Parasitol 2013;51(5):511-517.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.511

Abstract
PDF

Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.

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Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1: Wen Hui Li, Wan Zhong Jia, Zi Gang Qu, Zhi Zhou Xie, Jian Xun Luo, Hong Yin, Xiao Lin Sun, Radu Blaga, Bao Quan Fu; Korean J Parasitol 2013;51(2):197-201.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.197

Abstract
PDF

A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.

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