Parasites, Hosts and Diseases

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Brief Communications

Quantification of Immunoglobulin G against Trypanosoma cruzi in Individuals with Chronic Chagas Disease Treated with Nifurtimox and Evaluated in Prolonged Follow-Up: Gabriela Mun?oz, Camilo Vergara, Gabriela Marti?nez, Werner Apt, Ine?s Zulantay; Korean J Parasitol 2019;57(1):39-41.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.39

Abstract
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In the indeterminate chronic period of Chagas disease (ChD) the treatment has not been conclusive, because the serological negativization requires many years. This study aims to evaluate the efficacy of nifurtimox (NF) in the treatment of chronic ChD in prolonged follow-up by serological techniques of indirect immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA) IgG comparing 2 groups of patients, treated and non treated. Mann-Whitney test was performed for ELISA and IFA, with significant difference between the groups (P < 0.05). IgG levels were lower in individuals treated compared with untreated patients, indicating chemotherapeutic efficacy in prolonged follow-up.

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Changes in lipid abundance are associated with disease progression and treatment response in chronic Trypanosoma cruzi infection
Juan Carlos Gabaldón-Figueira, Albert Ros-Lucas, Nieves Martínez-Peinado, Gavin Blackburn, Irene Losada-Galvan, Elizabeth Posada, Cristina Ballart, Elisa Escabia, Jordi Capellades, Oscar Yanes, María-Jesús Pinazo, Joaquim Gascón, Julio Alonso-Padilla
Parasites & Vectors.2024;[Epub] CrossRef
Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study
Inés Zulantay, Gabriela Muñoz, Daniela Liempi, Tamara Rozas, María José Manneschi, Catalina Muñoz-San Martín, Carezza Botto-Mahan, Werner Apt, Gonzalo Cabrera
Pathogens.2022; 11(7): 787. CrossRef
Differential phenotypic and functional profile of epitope-specific cytotoxic CD8+ T cells in benznidazole-treated chronic asymptomatic Chagas disease patients
Adriana Egui, Manuel Carlos López, Inmaculada Gómez, Marina Simón, Manuel Segovia, M. Carmen Thomas
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.2020; 1866(3): 165629. CrossRef
Detection of Trypanosoma cruzi by PCR in adults with chronic Chagas disease treated with nifurtimox
Camilo Vergara, Gabriela Muñoz, Gabriela Martínez, Werner Apt, Inés Zulantay, Jude Marek Przyborski
PLOS ONE.2019; 14(8): e0221100. CrossRef
Chronic Chagas disease: Quantification of Trypanosoma cruzi in peripheral blood and dejections of Triatoma infestans fed by xenodiagnosis in patients with and without cardiopathy
Werner Apt, Daniela Carrasco, Cristian Fuentealba, Mauricio Canals, Gabriela Muñoz, Miguel Saavedra, Juan-Paul Castillo, Inés Zulantay
Acta Tropica.2019; 200: 105167. CrossRef

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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia: Sung-Hee Hong, Davaasuren Anu, Young-Il Jeong, Davaajav Abmed, Shin-Hyeong Cho, Won-Ja Lee, Sang-Eun Lee; Korean J Parasitol 2014;52(4):443-447.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.443

Abstract
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Babesiosis is an emerging tick-borne disease in humans worldwide; however, little is known about the frequency of infection or prevalence of this disease in other parts of the world, excluding North America. In this study, we aimed to investigate Babesia microti infection frequency in a human population in Mongolia. One hundred blood samples were collected from stock farmers living in Khutul city of Selenge province, Mongolia. The sera and DNA from blood samples were evaluated for the presence of B. microti infection by using indirect fluorescent antibody (IFA) tests and PCR. The positive detection rates obtained using the IFA tests and PCR assays were 7% and 3%, respectively. This study is the first to detect of B. microti infections based on antibody seroprevalence or PCR assays for the presence of B. microti DNA in a Mongolian population.

Citations

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Babesia microti Transmission Risk Through Ixodes persulcatus Ticks in Selenge, Mongolia, and Investigation of Its Infectivity in Rodents
Davganyam Bayarmagnai, Gombodash Ganbat, Ariunbold Munkhtsetseg, Mygarmarsuren Odonchimeg, Dashzevge Erdenechimeg, Tserennyam Davaajargal, Zorigt Uurtsaikh, Khandsuren Naranbaatar, Damdinsuren Boldbaatar, Bumduuren Tuvshintulga
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Transfusion-transmitted Babesia spp.: a changing landscape of epidemiology, regulation, and risk mitigation
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Journal of Clinical Microbiology.2023;[Epub] CrossRef
Global meta-analysis onBabesiainfections in human population: prevalence, distribution and species diversity
Solomon Ngutor Karshima, Magdalene Nguvan Karshima, Musa Isiyaku Ahmed
Pathogens and Global Health.2022; 116(4): 220. CrossRef
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Yi Yang, Jevan Christie, Liza Köster, Aifang Du, Chaoqun Yao
Microorganisms.2021; 9(2): 440. CrossRef
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Evan M. Bloch, Peter J. Krause, Laura Tonnetti
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Sabir Hussain, Abrar Hussain, Muhammad Umair Aziz, Baolin Song, Jehan Zeb, David George, Jun Li, Olivier Sparagano
Pathogens.2021; 11(1): 23. CrossRef
The Global Emergence of Human Babesiosis
Abhinav Kumar, Jane O’Bryan, Peter Krause
Pathogens.2021; 10(11): 1447. CrossRef
Hard ticks and tick-borne pathogens in Mongolia—A review
Jiří Černý, Buyantogtokh Buyannemekh, Tersia Needham, Gantulga Gankhuyag, Dashzeveg Oyuntsetseg
Ticks and Tick-borne Diseases.2019; 10(6): 101268. CrossRef
The Babesia observational antibody (BAOBAB) study: A cross-sectional evaluation of Babesia in two communities in Kilosa district, Tanzania
Evan M. Bloch, Zakayo Mrango, Mabula Kasubi, Jerusha Weaver, Aleksandra Mihailovic, Beatriz Munoz, Anna Weimer, Andrew Levin, Laura Tonnetti, Jeffrey M. Linnen, Vanessa Brès, Douglas E. Norris, Giovanna Carpi, Sheila K. West, Christine A Petersen
PLOS Neglected Tropical Diseases.2019; 13(8): e0007632. CrossRef
Some aspects on tick species in Mongolia and their potential role in the transmission of equine piroplasms, Anaplasma phagocytophilum and Borrelia burgdorferi L.
Myadagsuren Narankhajid, Chultemsuren Yeruult, Agvaandaram Gurbadam, Jigjav Battsetseg, Stephan W. Aberle, Badamdorj Bayartogtokh, Anja Joachim, Georg Gerhard Duscher
Parasitology Research.2018; 117(11): 3557. CrossRef
Case report of the patient source of the Babesia microti R1 reference strain and implications for travelers
Philipp Stahl, Yves Poinsignon, Pascal Pouedras, Vasilica Ciubotaru, Laurence Berry, Brinda Emu, Peter J Krause, Choukri Ben Mamoun, Emmanuel Cornillot
Journal of Travel Medicine.2018;[Epub] CrossRef
A pilot serosurvey of Babesia microti in Chinese blood donors
E. M. Bloch, Y. Yang, M. He, L. Tonnetti, Y. Liu, J. Wang, Y. Guo, H. Li, D. A. Leiby, H. Shan
Vox Sanguinis.2018; 113(4): 345. CrossRef
Babesia microti and Malaria Infection in Africa: A Pilot Serosurvey in Kilosa District, Tanzania
Evan M. Bloch, Mabula Kasubi, Andrew Levin, Zakayo Mrango, Jerusha Weaver, Beatriz Munoz, Sheila K. West
The American Journal of Tropical Medicine and Hygiene.2018; 99(1): 51. CrossRef
A novel quantitative PCR detects Babesia infection in patients not identified by currently available non-nucleic acid amplification tests
Lavoisier Akoolo, Samantha Schlachter, Rasel Khan, Laura Alter, Albert D. Rojtman, Kristine Gedroic, Purnima Bhanot, Nikhat Parveen
BMC Microbiology.2017;[Epub] CrossRef
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China
Zi-Hou Gao, Tao-Hua Huang, Bao-Gui Jiang, Na Jia, Zheng-Xiang Liu, Zong-Ti Shao, Rui-Ruo Jiang, Hong-Bo Liu, Ran Wei, Yu-Qiong Li, Hong-Wu Yao, Michael E. von Fricken, Jia-Fu Jiang, Chun-Hong Du, Wu-Chun Cao, Joseph M. Vinetz
PLOS Neglected Tropical Diseases.2017; 11(10): e0005898. CrossRef
Detection of Babesia venatorum, Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis in Ixodes persulcatus ticks from Mongolia
Carolin Karnath, Anna Obiegala, Stephanie Speck, Sandra Essbauer, Henri Derschum, Holger Scholz, Daniel Kiefer, Damdindorj Tserennorov, Otgonbataar Dashdavaa, Nyamdorj Tsogbadrakh, Battsetseg Jigjav, Martin Pfeffer
Ticks and Tick-borne Diseases.2016; 7(2): 357. CrossRef
Experimental transmission of Babesia microti by Rhipicephalus haemaphysaloides
Lan-Hua Li, Dan Zhu, Chen-Chen Zhang, Yi Zhang, Xiao-Nong Zhou
Parasites & Vectors.2016;[Epub] CrossRef
Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan
Aya Zamoto-Niikura, Shigeru Morikawa, Ken-Ichi Hanaki, Patricia J. Holman, Chiaki Ishihara, P. D. Schloss
Applied and Environmental Microbiology.2016; 82(22): 6624. CrossRef
The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia
Bumduuren Tuvshintulga, Thillaiampalam Sivakumar, Badgar Battsetseg, Sandag-ochir Narantsatsaral, Batsaikhan Enkhtaivan, Banzragch Battur, Kyoko Hayashida, Kazuhiro Okubo, Takahiro Ishizaki, Noboru Inoue, Ikuo Igarashi, Naoaki Yokoyama
Parasitology International.2015; 64(6): 527. CrossRef

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Original Article

Antibody reaction of human anti-Toxoplasma gondii positive and negative sera with Neospora caninum antigens: Ho-Woo Nam, Seung-Won Kang, Won-Young Choi; Korean J Parasitol 1998;36(4):269-275.
Published online December 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.4.269

Abstract
PDF

Anti-Neospora caninum antibody was detected in anti-Toxoplasma gondii positive and negative human sera by ELISA, western blot and immunofluorescence assay (IFA). Twelve cases out of 172 (6.7%) Toxoplasma-positive sera cross-reacted with both T. gondii and N. caninum antigens, and one out of 110 Toxoplasma-negative sera reacted with N. caninum antigen by ELISA. By western blot, all 12 sera reacted with T. gondii antigens with various banding patterns but specifically at 30 kDa (SAG1) and 22 kDa (SAG2) bands. With N. caninum antigen, the number of reactive bands was reduced, however a 43 kDa band reacted in three cases in Toxoplasma-positive sera in addition to one in Toxoplasma-negative control sera. All sera of the Toxoplasma-positive group labeled surface membrane of T. gondii, but reacted differently with N. caninum. Fluorescence was detected in surface membrane, subcellular organelles, or both in N. caninum. And one case in the Toxoplasma-negative group also reacted with N. caninum strongly in subcellular organelles. This suggested that the antibody against N. caninum may be present in human sera although the positive rate was very low in this study. The possibility of human infection with N. caninum remains to be evaluated further.

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Neospora caninum in pigs and pig farmers in India: Examining the prevalence, immunodominant antigens and associated risk factors
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Microbial Pathogenesis.2025; 200: 107352. CrossRef
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Tuğçe Tuygun, Serbülent Yiğit, Elif Burcu Gençay Topçu, Şinasi Umur
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Revista Brasileira de Parasitologia Veterinária.2021;[Epub] CrossRef
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Louis Lantier, Agathe Poupée-Beaugé, Anne di Tommaso, Céline Ducournau, Mathieu Epardaud, Zineb Lakhrif, Stéphanie Germon, Françoise Debierre-Grockiego, Marie-Noëlle Mévélec, Arthur Battistoni, Loïs Coënon, Nora Deluce-Kakwata-Nkor, Florence Velge-Roussel
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