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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia
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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia

Sung-Hee Hong1, Davaasuren Anu2, Young-Il Jeong1, Davaajav Abmed2, Shin-Hyeong Cho1, Won-Ja Lee1, Sang-Eun Lee1
The Korean Journal of Parasitology 2014;52(4):443-447.
Published online: August 29, 2014

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Corresponding author (ondalgl@cdc.go.kr)
• Received: November 13, 2013   • Revised: May 21, 2014   • Accepted: June 24, 2014

© 2014, Korean Society for Parasitology and Tropical Medicine

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  • Babesiosis is an emerging tick-borne disease in humans worldwide; however, little is known about the frequency of infection or prevalence of this disease in other parts of the world, excluding North America. In this study, we aimed to investigate Babesia microti infection frequency in a human population in Mongolia. One hundred blood samples were collected from stock farmers living in Khutul city of Selenge province, Mongolia. The sera and DNA from blood samples were evaluated for the presence of B. microti infection by using indirect fluorescent antibody (IFA) tests and PCR. The positive detection rates obtained using the IFA tests and PCR assays were 7% and 3%, respectively. This study is the first to detect of B. microti infections based on antibody seroprevalence or PCR assays for the presence of B. microti DNA in a Mongolian population.
  • Key words: Babesia microti, IFA test, PCR, Mongolia
Babesiosis, caused by an intraerythrocytic protozoan parasite, is a common disease in wildlife worldwide and is gaining attention as an emerging tick-borne zoonosis in humans [1,2]. Although approximately 100 Babesia species infect animals, only a few have been reported to infect humans [3,4]. Most cases of human babesiosis have been caused by Babesia divergens, Babesia microti, or Babesia duncani, with the cattle-infecting species B. divergens being the most common in Europe, and the rodent-infecting species B. microti and B. duncani being the most common in North America. Together, these species have caused a significant number of human infections [1,5]. Several cases of B. duncani and B. duncani-type organisms have been identified in patients on the Pacific coast of the United States from northern California to Washington state [6,7]. B. microti is a parasite in wild rodents, and most cases of babesiosis in humans caused by B. microti are transmitted from wild rodents through Ixodes tick bites or inadvertently during blood transfusions [8,9]. In Asia, B. microti-like parasites have caused illnesses in Japan and Taiwan [10,11], and a novel Babesia sp. (KO1) has been identified in South Korea [12].
Mongolia, situated in central Asia between Russia to the north and China to the south, is an agricultural country focused on the livestock industry. Animal husbandry of cattle, sheep, goats, and horses brings many people into contact with ticks while herding the livestock pasturing on steppes. Previous reports have confirmed the presence of Babesia gene fragments from parasitic species in field-collected Dermacentor nuttalli ticks in Mongolia [13], and babesiosis in Mongolian horses is widespread [14]. Also, B. microti DNA was reportedly detected in small mammals in Russia [16] and in ticks of Inner Mongolia [17]. Furthermore, the highest incidence of human Lyme borreliosis transmitted by tick has been reported in Selenge province [15].
Similarly, previous reports of B. microti infection in Mongolia are limited to ticks and animals, and minimal information exists regarding B. microti infection among the Mongolian population involved in the livestock industry, even though they are highly exposed in the B. microti infection. The objective of this study was to serologically and molecularly detect B. microti infection in small stock farmers in Khutul city of Selenge province in northern Mongolia, which is adjacent to neighboring regions, Russia.
One hundred blood samples were collected in April 2011 from residential stock farmers living in Khutul, near the Russian border (Fig. 1). Genomic DNA was extracted from the blood samples using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions and was stored in 100 µl of Tris-EDTA at 20℃. The blood samples were separated by centrifugation at 2,000 g for 10 min, and the collected sera were stored at 20℃ until use.
The DNA samples were used as templates for a nested-PCR to amplify the B. microti 18S ribosomal ribonucleic acid (18S rRNA) gene [18]. The PCR primers included the 1st forward (5'-GCCAGTAGTCATATGCTTGTGTTA-3') and reverse (5'-CTCCTTCCTY TAAGTGATAAGGTTCAC-3') for the initial reactions and 2nd forward (5'-CCATGCAT GTCTWAGTAYAARCTTTTA-3') and reverse (5'-CCTYTAAGTGATAAGGTTCACAA AACTT-3') for the subsequent reactions. Amplifications were performed in 20-µl reactions by using AccuPower PCR master mix (Bioneer, Daejeon, Korea) containing 1 µM each of the 1st forward and reverse primers, sterile water, and 1 µg of DNA sample. The second PCR was then performed using 2 µl of the 1st PCR product and the 2nd PCR primers. The thermal cycler conditions were 94℃ for 5 min; 35 cycles of 94℃ for 60 sec, 59℃ for 45 sec, and 72℃ for 45 sec; and a final extension at 72℃ for 10 min. Amplification products were resolved by electrophoresis on 1.5% agarose gels in Tris-acetate-EDTA buffer and were visualized using Safe-Pinky DNA gel staining solution (GenDepot, Houston, Texas, USA). The positive PCR products were then purified using an agarose gel DNA purification kit (Qiagen) and sequenced using an ABI PRISM 3730xl Analyzer (Applied Biosystems, Foster City, California, USA).
IgG antibodies to B. microti were detected in the serum samples by using an indirect fluorescent antibody (IFA) test kit (Focus Technologies, Inc., Cypress, California, USA). A cut-off value of 1:64 was used in accordance with the manufacturer's recommendations. The serum samples were diluted 64-fold in PBS, and 25 µl of each was placed on slides coated with fixed B. microti antigen derived from RBCs from B. microti-infected hamsters. Proper positive and negative controls in IFA test kit were performed in all testing. The slides were incubated in a humid environment at 37℃ for 30 min and washed 5 times with PBS. Diluted fluorescent-labeled anti-human IgG conjugate was placed on the slides before they were incubated in a humid environment at 37℃ for 30 min. The slides were washed 5 times with PBS and air-dried prior to examination by fluorescence microscopy at×400 magnification. Positive samples were consecutively diluted 2-fold to establish the endpoint titer. The IFA test detected antibodies to B. microti in 7% of the samples, with reported titers ranging between 64 and 1,024. The PCR assay detected B. microti 18S rRNA in 3% of the samples. Three of the samples tested positive in both assays, while 4 were seropositive in the IFA test but negative in the PCR assay (Table 1). The PCR-positive samples had IFA titers of both 512 and 1,024. Sequencing results showed that all PCR-positive samples were similar to B. microti. One of the 2 sequences obtained from positive samples were similar to a Clethrionomys sp. isolated from Russia (AY144693) and the other was similar to a human isolate from China (KF4110827). Five of the 7 B. microti-positive samples were from females, and 2 additional positive results were obtained for subjects of unknown sex (Table 2). No statistical differences were observed between the positive and negative results with regard to age and history of tick bites.
Some parasitic Babesia isolates that are extensively distributed in Mongolia are considered pathogenic only in animals, such as B. equi (EMA-1) from pasture ticks and B. microti (BC48) [14,19] and B. bigemina from Mongolian cattle [20]. Different parasitic Babesia spp. can cause babesiosis in humans, resulting in distinct geographical distributions determined by the presence of the intermediate hosts. In Eurasia, babesiosis caused by B. divergens is rare, but it is more lethal to human and bovine hosts [1,21] than North American hosts, infected predominantly by B. microti [22].
Our study is the first to detect B. microti in human subjects in Mongolia by utilizing seroprevalence and molecular assays, and our results may provide insights into the diagnosis and control of this disease in Mongolia. A broader and more detailed survey for other human infections of zoonotic Babesia spp., such as B. divergens and B. duncani is warranted in other regions of Mongolia. Traditionally, the diagnosis of babesiosis in humans has been based on detection of parasites in blood smears from patients with clinical symptoms of the disease. Because the clinical symptoms of babesiosis is usually mild in humans and the infection rate of RBCs is less than 1% [23,24], it is difficult to diagnose babesiosis by microscopic examination. The absence or presence of a laboratory manifestation depends on the level of parasitemia in the host patient.
Although B. microti is widely distributed throughout the world, symptomatic infections in humans are seldom reported [25]. In the present study, none of the individuals who tested positive exhibited the associated clinical symptoms of fever or anemia. Another difficulty with diagnosing Babesia-specific antibodies in human sera has been the lack of commercially available standardized tests for use in diagnostic laboratories [26]. However, the IFA tests now available have been shown to be sensitive, specific, and reproducible methods for detecting B. microti-specific antibodies in human sera [27]. In this study, we evaluated a commercially available IFA test to detect antibodies to B. microti in the sera of individuals. In addition, we screened specifically for the B. microti 18S rRNA gene by analyzing blood samples by using a PCR assay. The results of the IFA tests and PCR assays were similar, suggesting that both methods could be utilized for diagnosing B. microti infections in humans. Poyraz et al. [28] previously reported that no statistical differences exist in the seropositivity between sexes or age groups in Turkey, the same results found in our study. Another study found that the prevalence of antibodies to B. microti or B. divergens was considerably higher in tick-exposed individuals than that found in a control group of healthy blood donors [27].
Interestingly, our study found no significant differences in the prevalence of B. microti infections between individuals who had been bitten by ticks and those who had not. This suggests the need for an investigation into what vector species carrying B. microti, including ticks, are present in Khutul, Mongolia. This study is the first to report B. microti infections among stock farmers in Khutul city of Selenge Province, Mongolia, based on antibody seroprevalence and a PCR assay for the presence of B. microti 18S rRNA in the blood of subjects. Although no correlation was found between positive tests for infection and tick bite exposure, the risk of infection from tick-borne diseases may still exist in this region. The IFA test and PCR assay proved to be useful tools for screening of B. microti infections, and further serological and molecular epidemiological surveys should be performed with larger sample sizes throughout Mongolia. These studies should be conducted in conjunction with an investigation of the prevalence of ticks and rodents in order to determine which species is the primary B. microti infection vector in Mongolia.
Korea National Institute of Health, Korea Centers for Disease Control and Prevention4847-302-210-13, 2011

We have no conflict of interest related to this study.

This research was performed in collaboration with the Korea CDC and Mongolia NCCD and funded by a grant (4847-302-210-13, 2011) from the Korea National Institute of Health, Korea Centers for Disease Control and Prevention.
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Fig. 1
The survey region for Babesia microti infections among stock farmers living in Khutul City in Selenge Province, northern Mongolia.
kjp-52-443-g001.jpg
Table 1.
Indirect fluorescent antibody (IFA) and PCR assay results for Babesia microti infections among residents of Khutul city in Selenge province, Mongolia
Table 1.
Number tested Total no. positive in IFA Titer Total no. positive in PCR No. positive in both IFA and PCR
100 ≥ 64 64 128 256 512 > 1,024 3 (3%) 3 (3%)
7 (7%) 3 1 0 2 1
Table 2.
Positive rate of B. microti in IFA and PCR according to age, gender, and tick bite exposure
Table 2.
Variables No. Positive (%) for B. microti in IFA Positive (%) for B. microti in PCR
Age (years)
 < 10 2 - -
 11−20 16 2 (12.5) 1 (6.3)
 21−30 16 - -
 31−40 15 - -
 41−50 17 1 (5.9) 1 (5.9)
 60 > 16 1 (6.3) -
 Unknown 18 3 (16.7) 1 (5.6)
Sex
 Male 42 - -
 Female 47 5 (10.6) 2 (4.3)
 Unknown 11 2 (18.2) 1 (9.1)
Tick bite exposure
 Yes 59 3 (5.1) 2 (3.4)
 No 41 4 (9.8) 1 (2.4)
Total 100 7 (7) 3 (3)

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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia
Korean J Parasitol. 2014;52(4):443-447.   Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.443
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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia
Korean J Parasitol. 2014;52(4):443-447.   Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.443
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Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia
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Fig. 1 The survey region for Babesia microti infections among stock farmers living in Khutul City in Selenge Province, northern Mongolia.

Fig. 1

Molecular Detection and Seroprevalence of Babesia microti among Stock Farmers in Khutul City, Selenge Province, Mongolia
Number tested Total no. positive in IFA Titer Total no. positive in PCR No. positive in both IFA and PCR 100 ≥ 64 64 128 256 512 > 1,024 3 (3%) 3 (3%) 7 (7%) 3 1 0 2 1 Variables No. Positive (%) for B. microti in IFA Positive (%) for B. microti in PCR Age (years)  < 10 2 - -  11−20 16 2 (12.5) 1 (6.3)  21−30 16 - -  31−40 15 - -  41−50 17 1 (5.9) 1 (5.9)  60 > 16 1 (6.3) -  Unknown 18 3 (16.7) 1 (5.6) Sex  Male 42 - -  Female 47 5 (10.6) 2 (4.3)  Unknown 11 2 (18.2) 1 (9.1) Tick bite exposure  Yes 59 3 (5.1) 2 (3.4)  No 41 4 (9.8) 1 (2.4) Total 100 7 (7) 3 (3)
Table 1. Indirect fluorescent antibody (IFA) and PCR assay results for Babesia microti infections among residents of Khutul city in Selenge province, Mongolia
Table 2. Positive rate of B. microti in IFA and PCR according to age, gender, and tick bite exposure

Table 1.

Table 2.

PHD : Parasites, Hosts and Diseases

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