Parasites, Hosts and Diseases

"IL-4"

Original Articles

Experimental Murine Fascioliasis Derives Early Immune Suppression with Increased Levels of TGF-β and IL-4: Joon-Yong Chung, Young-An Bae, Doo-Hee Yun, Hyun-Jong Yang, Yoon Kong; Korean J Parasitol 2012;50(4):301-308.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.301

Abstract
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In fascioliasis, T-helper 2 (Th2) responses predominate, while little is known regarding early immune phenomenon. We herein analyzed early immunophenotype changes of BALB/c, C57BL/6, and C3H/He mice experimentally infected with 5 Fasciola hepatica metacercariae. A remarkable expansion of CD19⁺ B cells was observed as early as week 1 post-infection while CD4⁺/CD8⁺ T cells were down-regulated. Accumulation of Mac1⁺ cells with time after infection correlated well with splenomegaly of all mice strains tested. The expression of tumor necrosis factor (TNF)-α mRNA in splenocytes significantly decreased while that of IL-4 up-regulated. IL-1β expression was down-modulated in BALB/c and C57BL/6 mice, but not in C3H/He. Serum levels of transforming growth factor (TGF)-β were considerably elevated in all mice during 3 weeks of infection period. These collective results suggest that experimental murine fascioliasis might derive immune suppression with elevated levels of TGF-β and IL-4 during the early stages of infection.

Citations

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Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica
María Teresa Ruiz-Campillo, Isabel Lourdes Pacheco, Nieves Abril, María José Bautista, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Leandro Buffoni, José Pérez, Verónica Molina-Hernández, Rafael Zafra
Veterinary Research.2024;[Epub] CrossRef
Fascioliasis: Image Findings, Diagnosis, and Treatment
Jae Seung Lee
Clinical Ultrasound.2024; 9(1): 18. CrossRef
Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
Luis Miguel Flores-Velázquez, María Teresa Ruiz-Campillo, Guillem Herrera-Torres, Álvaro Martínez-Moreno, Francisco Javier Martínez-Moreno, Rafael Zafra, Leandro Buffoni, Pablo José Rufino-Moya, Verónica Molina-Hernández, José Pérez
Frontiers in Veterinary Science.2023;[Epub] CrossRef
The immunosuppression effects of deforolimus (ridaforolimus, AP23573) on allograft organ transplantation
Lumin Wang, Yanping Li, Dawei Yang, Jiazhao Fu, Bin Zhao, Yaguang Li, Yanrong Ye, Zhongquan Qi
Clinical and Translational Discovery.2023;[Epub] CrossRef
Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge
Yongqian Bu, Caiwen Jia, Xiaowei Tian, Kalibixiati Aimulajiang, Muhammad Ali Memon, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
Pathogens.2020; 9(1): 46. CrossRef
Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica
María Teresa Ruiz-Campillo, Verónica Molina-Hernández, María José Bautista, Isabel L. Pacheco, Rafael Zafra, Leandro Buffoni, Francisco Javier Martínez-Moreno, Alvaro Martínez-Moreno, José Pérez
Veterinary Research.2020;[Epub] CrossRef
Helminth infection-induced carcinogenesis: spectrometric insights from the liver flukes, Opisthorchis and Fasciola
Maria João Gouveia, Maria Y. Pakharukova, Gabriel Rinaldi, Viatcheslav A. Mordvinov, Paul J. Brindley, Fátima Gärtner, Nuno Vale, Martin Michaelis
Experimental Results.2020;[Epub] CrossRef
Fasciola and fasciolosis in ruminants in Europe: Identifying research needs
N. J. Beesley, C. Caminade, J. Charlier, R. J. Flynn, J. E. Hodgkinson, A. Martinez-Moreno, M. Martinez-Valladares, J. Perez, L. Rinaldi, D. J. L. Williams
Transboundary and Emerging Diseases.2018; 65: 199. CrossRef
Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
Isabel L. Pacheco, Nieves Abril, Rafael Zafra, Verónica Molina-Hernández, Noelia Morales-Prieto, María J. Bautista, María T. Ruiz-Campillo, Raúl Pérez-Caballero, Alvaro Martínez-Moreno, José Pérez
Veterinary Research.2018;[Epub] CrossRef
Association of Fasciola hepatica Infection with Liver Fibrosis, Cirrhosis, and Cancer: A Systematic Review
Claudia Machicado, Jorge D. Machicado, Vicente Maco, Angelica Terashima, Luis A. Marcos, Hector H Garcia
PLOS Neglected Tropical Diseases.2016; 10(9): e0004962. CrossRef

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IL-4 Independent Nuclear Translocalization of STAT6 in HeLa Cells by Entry of Toxoplasma gondii: Hye-Jin Ahn, Ji Yeon Kim, Ho-Woo Nam; Korean J Parasitol 2009;47(2):117-124.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.117

Abstract
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Toxoplasma gondii provokes rapid and sustained nuclear translocation of the signal transducer and activator of transcription 6 (STAT6) in HeLa cells. We observed activation of STAT6 as early as 2 hr after infection with T. gondii by the nuclear translocation of fluorescence expressed from exogenously transfected pDsRed2-STAT6 plasmid and by the detection of phosphotyrosine-STAT6 in Western blot. STAT6 activation occurred only by infection with live tachyzoites but not by co-culture with killed tachyzoites or soluble T. gondii extracts. STAT6 phosphorylation was inhibited by small interfering RNA of STAT6 (siSTAT6). In view of the fact that STAT6 is a central mediator of IL-4 induced gene expression, activation of STAT6 by T. gondii infection resembles that infected host cells has been stimulated by IL-4 treatment. STAT1 was affected to increase the transcription and expression by the treatment of siSTAT6. STAT6 activation was not affected by any excess SOCS's whereas that with IL-4 was inhibited by SOCS-1 and SOCS-3. T. gondii infection induced Eotaxin-3 gene expression which was reduced by IFN-γ. These results demonstrate that T. gondii exploits host STAT6 to take away various harmful reactions by IFN-γ. This shows, for the first time, IL-4-like action by T. gondii infection modulates microbicidal action by IFN-γ in infected cells.

Citations

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Nuclear Localization and Cleavage of STAT6 Is Induced by Kaposi’s Sarcoma-Associated Herpesvirus for Viral Latency
Chong Wang, Caixia Zhu, Fang Wei, Shujun Gao, Liming Zhang, Yuhong Li, Yanling Feng, Yin Tong, Jianqing Xu, Bin Wang, Zhenghong Yuan, Erle S. Robertson, Qiliang Cai, Paul D. Ling
PLOS Pathogens.2017; 13(1): e1006124. CrossRef
SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection
Gang Lu, Jian Zhou, Aihua Zhou, Yali Han, Jingjing Guo, Pengxia Song, Huaiyu Zhou, Hua Cong, Ming Hou, Lin Wang, Shenyi He
Parasitology International.2017; 66(5): 596. CrossRef
SAG4 DNA and Peptide Vaccination Provides Partial Protection against T. gondii Infection in BALB/c Mice
Jian Zhou, Lin Wang
Frontiers in Microbiology.2017;[Epub] CrossRef
Afatinib Reduces STAT6 Signaling of Host ARPE-19 Cells Infected with Toxoplasma gondii
Zhaoshou Yang, Hye-Jin Ahn, Young-Hoon Park, Ho-Woo Nam
The Korean Journal of Parasitology.2016; 54(1): 31. CrossRef
Chronic Toxoplasmosis Modulates the Induction of Contact Hypersensitivity by TNCB in Mouse Model
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2015; 53(6): 755. CrossRef
Activation of STAT6 by STING Is Critical for Antiviral Innate Immunity
Huihui Chen, Hui Sun, Fuping You, Wenxiang Sun, Xiang Zhou, Lu Chen, Jing Yang, Yutao Wang, Hong Tang, Yukun Guan, Weiwei Xia, Jun Gu, Hiroki Ishikawa, Delia Gutman, Glen Barber, Zhihai Qin, Zhengfan Jiang
Cell.2011; 147(2): 436. CrossRef
Deactivation of STAT6 through Serine 707 Phosphorylation by JNK
Takashi Shirakawa, Yoshinori Kawazoe, Tomoko Tsujikawa, Dongju Jung, Shin-ichi Sato, Motonari Uesugi
Journal of Biological Chemistry.2011; 286(5): 4003. CrossRef
Coactivator P100 Protein Enhances STAT6‐Dependent Transcriptional Activation But Has No Effect on STAT1‐Mediated Gene Transcription
Xinting Wang, Xin Liu, Jianfei Fang, Yanxin Lu, Jinyan He, Xuyang Yao, Zhi Yao, Jie Yang
The Anatomical Record.2010; 293(6): 1010. CrossRef
Toxoplasma Rhoptry Protein 16 (ROP16) Subverts Host Function by Direct Tyrosine Phosphorylation of STAT6
Yi-Ching Ong, Michael L. Reese, John C. Boothroyd
Journal of Biological Chemistry.2010; 285(37): 28731. CrossRef
STAT6 activation by Toxoplasma gondii infection induces the expression of Th2 C-C chemokine ligands and B clade serine protease inhibitors in macrophage
Hye-Jin Ahn, Ji Yeon Kim, Kyung-Ju Ryu, Ho-Woo Nam
Parasitology Research.2009; 105(5): 1445. CrossRef