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"Nguyen Van De"

Case Report

Two Human Cases of Echinococcus ortleppi Infection in the Lung and Heart in Vietnam
Nguyen Van De, Pham Ngoc Minh, Le Van Duyet, Nguyen Ngoc Bich, Trinh Nam Son, Bong-Kwang Jung, Jong-Yil Chai
Korean J Parasitol 2020;58(4):451-456.
Published online August 25, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.4.451
This is a report of 2 cases of human hydatidosis caused by Echinococcus ortleppi in Vietnam. The patients were a 12-year-old male (case 1) having a cyst of 10.0×9.0 cm size in the lung and a 50-year-old female with a 3.0×3.3 cm-sized cyst in the heart. Eosinophilia was 33.7% in the male and 45.8% in the female patient. C-reactive protein was increased to 16.5 mg/L in the male and 18.2 mg/L in the female. Both patients were positive for ELISA at OD=2.5 and 3.1, respectively. Echinococcus protoscolices were collected from the cysts by amniocentesis and surgery. The protoscolices were identified as E. ortleppi by morphology and analysis of mitochondrial NADH dehydrogenase 1 (nad1) gene sequence. Both patients were cured by surgical resection of the hydatid cyst combined with albendazole medication. The E. ortleppi infection in lung is the second report, and the other in the heart is the first in Vietnam.

Citations

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  • Current Perspectives on Cystic Echinococcosis: A Systematic Review
    Hawkar A. Nasralla, Berun A. Abdalla, Hiwa O. Abdullah, Sasan M. Ahmed, Fahmi H. Kakamad, Shvan H. Mohammed, Rawezh Q. Salih, Dahat A. Hussein, Tomas M. Mikael, Marwan N. Hassan, Hunar A. Hassan, Suhaib H. Kakamad, Kayhan A. Najar, Karukh K.
    Judi Clinical Journal.2025; 1(1): 12.     CrossRef
  • Epidemiological study on bovine cystic echinococcosis: Abattoir survey, cyst characterization and its economic impact at Mekaneyesuse municipality abattoir, Northwest Ethiopia
    Belete Sendekie, Beyenech Gebeyehu, Abebe Mihret, Tesfu Kassa, Nigatu Kebede
    Parasite Epidemiology and Control.2024; 25: e00355.     CrossRef
  • Echinococcus ortleppi infection in humans: An emerging zoonosis in Asia
    Ayako Yoshida, Takao Irie, Lan-Anh Le, Khanh-Linh Bui, Yukifumi Nawa
    Parasitology International.2024; 103: 102949.     CrossRef
  • An overview of human helminthioses in Vietnam: Their prevention, control and lessons learnt
    Hung Manh Nguyen, Dung Trung Do, Stephen E. Greiman, Ha Van Nguyen, Hien Van Hoang, Toan Quoc Phan, Phuc Pham-Duc, Henry Madsen
    Acta Tropica.2023; 238: 106753.     CrossRef
  • First report of Echinococcus ortleppi and genotype G6 of E. canadensis cluster from southern Punjab, Pakistan and a global overview on genetic structure and host adaptation of E. ortleppi
    Hira Muqaddas, Naunain Mehmood, Urmas Saarma, Ammarah Usman, Fahad Ahmed, Antonio Varcasia, Maria Francesca Sini, Muhammad Irfan Ullah
    Acta Tropica.2023; 244: 106951.     CrossRef
  • Vietnam: Neglected tropical diseases in an emerging and accelerating economy
    Kala Pham, Peter J Hotez, Aaron R. Jex
    PLOS Neglected Tropical Diseases.2022; 16(2): e0010140.     CrossRef
  • Species and genotypes belonging to Echinococcus granulosus sensu lato complex causing human cystic echinococcosis in Europe (2000–2021): a systematic review
    Adriano Casulli, Alessandro Massolo, Urmas Saarma, Gérald Umhang, Federica Santolamazza, Azzurra Santoro
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Cardiac hydatid cysts in a young man: A case report and a literature review
    Mircea Bajdechi, Dalia Manolache, Adrian Tudor, Mihnea Orghidan, Adriana Gurghean
    Experimental and Therapeutic Medicine.2022;[Epub]     CrossRef
  • Genetic Characterization of Echinococcus granulosus Sensu Lato in Livestock and Human Isolates from North of Iran Indicates the Presence of E. ortleppi in Cattle
    Khadijeh Nematdoost, Keyhan Ashrafi, Bijan Majidi-Shad, Eshrat Beigom Kia, Arash Zeinali, Meysam Sharifdini
    Acta Parasitologica.2021; 66(2): 446.     CrossRef
  • First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) from Portugal
    Teresa Letra Mateus, Maria João Gargaté, Anabela Vilares, Idalina Ferreira, Manuela Rodrigues, Catarina Coelho, Madalena Vieira-Pinto
    Microorganisms.2021; 9(6): 1256.     CrossRef
  • Extracellular vesicles of Echinococcus granulosus have therapeutic effects in allergic airway inflammation
    Mi Jin Jeong, Shin Ae Kang, Jun Ho Choi, Da In Lee, Hak Sun Yu
    Parasite Immunology.2021;[Epub]     CrossRef
  • Terminologie à utiliser pour l’étude et la prise en charge des échinococcoses : adaptation du consensus international à la langue française
    A.P. Bellanger, K. Achour, S. Barkati, C. Bastid, S. Bresson-Hadni, B. Delaere, C. Dziri, B. Gottstein, M. Kachani, G. Mantion, G. Umhang, M. Wallon, D.A. Vuitton
    Bulletin de l'Académie Nationale de Médecine.2021; 205(8): 1028.     CrossRef
  • 6,448 View
  • 96 Download
  • 10 Web of Science
  • Crossref

Original Articles

Seroprevalence of Tissue and Luminal Helminths among Patients in Hanoi Medical University Hospital, Vietnam, 2018
Nguyen Van De, Pham Ngoc Minh, Nguyen Ngoc Bich, Jong-Yil Chai
Korean J Parasitol 2020;58(4):387-392.
Published online August 25, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.4.387
A serological survey was performed using ELISA to estimate the prevalence of tissue and luminal helminthic infections among hospital patients in Hanoi region, Vietnam. An overall seroprevalence of tissue and luminal helminthiases was 64.0% (95% CI 61.2-66.8) among 1,120 patients who visited Hanoi Medical University Hospital, Vietnam in 2018. The highest seroprevalence was observed against Toxocara spp. (59.0%), followed by Strongyloides stercoralis (46.3%), Gnathostoma spp. (25.5%), cysticercus (12.8%), Angiostrongylus cantonensis (10.5%), Fasciola spp. (11.1%), and Clonorchis sinensis (8.7%). Mono-infection by one species (11.1%) was lower than multiple infections (53.0%) (P<0.05). The seroprevalence in males (59.3%) was lower than in females (66.2%) but not statistically significant (P>0.05). Children (<15 years) revealed lower seroprevalence (34.0%) than adults (68.4%), and the age group 51-70 years revealed the highest seroprevalence (76.0%). Among the seropositive patients, eosinophilia (≥8.0%) was noted in 80.2%. The present results suggested active transmission of various tissue and luminal helminths among people in Hanoi, Vietnam.

Citations

Citations to this article as recorded by  Crossref logo
  • Seroprevalence and associated risk factors of human fascioliasis in Yen Bai and Thanh Hoa provinces, Vietnam
    Thao Thi Bich Nguyen, Hafid Dahma, Veronique Dermauw, Dung Thi Bui, Bertrand Losson, Pierre Dorny, Dung Trung Do, Olivier Vandenberg
    Parasitology International.2026; 112: 103219.     CrossRef
  • Toxocara seroprevalence among suspected patients across different geographical regions in Vietnam: A retrospective study
    Trinh Thi Que, Tran Van Chieu, Trieu Thuy Anh, Pham Van Ngai, Pham Van Tran, Nguyen Thai Son, Do Ngoc Anh
    Journal of Microbiology, Immunology and Infection.2025;[Epub]     CrossRef
  • The seroprevalence of toxocariasis and related risk factors in children in Ho Chi Minh City, Vietnam: results from a school-based cross-sectional study
    Thieu V Ha, Tien T N Vo, Duy K H Dang, Y M L Tran, Thanh V Kim, Duc H Le, Lan-Anh T Do, Hong K Tang
    Transactions of The Royal Society of Tropical Medicine and Hygiene.2024; 118(6): 384.     CrossRef
  • Fasciola spp. in Southeast Asia: A systematic review
    Vinh Hoang Quang, Bruno Levecke, Dung Do Trung, Brecht Devleesschauwer, Binh Vu Thi Lam, Kathy Goossens, Katja Polman, Steven Callens, Pierre Dorny, Veronique Dermauw, Krystyna Cwiklinski
    PLOS Neglected Tropical Diseases.2024; 18(1): e0011904.     CrossRef
  • Fascioliasis, una zoonosis emergente en Perú y el mundo: factores asociados a la infección en niños de edad escolar
    A. Valderrama–Pomé
    Revista de la Facultad de Medicina Veterinaria y de Zootecnia.2023;[Epub]     CrossRef
  • Prevalence of Strongyloides in Southeast Asia: a systematic review and meta-analysis with implications for public health and sustainable control strategies
    Abigail Hui En Chan, Teera Kusolsuk, Dorn Watthanakulpanich, Wallop Pakdee, Pham Ngoc Doanh, Azlin Mohd Yasin, Paron Dekumyoy, Urusa Thaenkham
    Infectious Diseases of Poverty.2023;[Epub]     CrossRef
  • Seroprevalence of toxocariasis among allergic patients in Kuwait and its association with eosinophilia
    Mohammad Al-Awadhi, Wafaa Jamal
    Parasite Epidemiology and Control.2022; 18: e00260.     CrossRef
  • Human Fascioliasis: Current Epidemiological Status and Strategies for Diagnosis, Treatment, and Control


    Maria Alejandra Caravedo, Miguel Cabada
    Research and Reports in Tropical Medicine.2020; Volume 11: 149.     CrossRef
  • 5,569 View
  • 112 Download
  • 9 Web of Science
  • Crossref
Distribution Status of Hybrid Types in Large Liver Flukes, Fasciola Species (Digenea: Fasciolidae), from Ruminants and Humans in Vietnam
Thi Bich Nga Nguyen, Nguyen Van De, Thi Kim Lan Nguyen, Huynh Hong Quang, Huong Thi Thanh Doan, Takeshi Agatsuma, Thanh Hoa Le
Korean J Parasitol 2018;56(5):453-461.
Published online October 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.5.453
The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 human specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L-(n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n=36) of the S- and 15% (n=6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n=4) of the S- and 7.5% (n=3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are morphologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.

Citations

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  • Integrating hybridization and introgression into host–parasite epidemiology, ecology, and evolution
    Ben Lukubye, David J. Civitello
    Trends in Parasitology.2025; 41(2): 129.     CrossRef
  • Meta-analysis and systematic review of the prevalence and risk factors of animal fascioliasis in Eastern and Southern Africa between 2000 and 2023
    Stellah Nambuya, Chester Kalinda, Patrick Vudriko, Moses Adriko, Million Phiri, Tafadzwa Mindu, David Wagaba, Lawrence Mugisha
    Preventive Veterinary Medicine.2025; 239: 106490.     CrossRef
  • Challenges in the recognition of trematode species: Consideration of hypotheses in an inexact science
    T.H. Cribb, D.P. Barton, D. Blair, N.J. Bott, R.A. Bray, R.D. Corner, S.C. Cutmore, M.L.I. De Silva, B. Duong, A. Faltýnková, A. Gonchar, R.F. Hechinger, K.K. Herrmann, D.C. Huston, P.T.J. Johnson, G. Kremnev, R. Kuchta, C. Louvard, W.J. Luus-Powell, S.B.
    Journal of Helminthology.2025;[Epub]     CrossRef
  • Human fascioliasis emergence in southern Asia: Complete nuclear rDNA spacer and mtDNA gene sequences prove Indian patient infection related to fluke hybridization in northeastern India and Bangladesh
    M. Dolores Bargues, Patricio Artigas, George M. Varghese, T. Jacob John, Sitara S.R. Ajjampur, Syed Ali Ahasan, Emdadul Haque Chowdhury, Albis Francesco Gabrielli, Santiago Mas-Coma
    One Health.2024; 18: 100675.     CrossRef
  • Environmental influence on abundance and infection patterns of snail intermediate hosts of liver and intestinal flukes in North and Central Vietnam
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    Parasitology Research.2024;[Epub]     CrossRef
  • One Health monitoring reveals invasive freshwater snail species, new records, and undescribed parasite diversity in Zimbabwe
    Aspire Mudavanhu, Ruben Schols, Emilie Goossens, Tamuka Nhiwatiwa, Tawanda Manyangadze, Luc Brendonck, Tine Huyse
    Parasites & Vectors.2024;[Epub]     CrossRef
  • A multidisciplinary analysis of over 53,000 fascioliasis patients along the 1995–2019 countrywide spread in Vietnam defines a new epidemiological baseline for One Health approaches
    Nguyen Van De, Pham Ngoc Minh, Thanh Hoa Le, Do Trung Dung, Tran Thanh Duong, Bui Van Tuan, Le Thanh Dong, Nguyen Van Vinh Chau, Pablo F. Cuervo, M. Dolores Bargues, M. Adela Valero, Albis Francesco Gabrielli, Antonio Montresor, Santiago Mas-Coma
    One Health.2024; 19: 100869.     CrossRef
  • Fasciola hepatica and Fasciola hybrid form co-existence in yak from Tibet of China: application of rDNA internal transcribed spacer
    Wenqiang Tang, Yule Zhou, Leyi Li, Bin Shi, Xialing Zhao, Kai Li, Wenting Chui, Jun Kui, Fuqiang Huang
    Parasitology Research.2024;[Epub]     CrossRef
  • First morphometric and molecular characterization of Fasciola spp. in Northwest Tunisia
    Ines Hammami, Lavina Ciuca, Maria Paola Maurelli, Rihab Romdhane, Limam Sassi, Mohamed Ridha Rjeibi, Nadia Farhat, Alain Kouam Simo, Laura Rinaldi, Mourad Rekik, Mohamed Gharbi
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  • One Health for fascioliasis control in human endemic areas
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  • Wide variation of heterozygotic genotypes of recent fasciolid hybrids from livestock in Bangladesh assessed by rDNA internal transcribed spacer region sequencing and cloning
    Syed Ali Ahasan, Alejandra De Elías-Escribano, Patricio Artigas, Mohammad Zahangir Alam, M. Motahar Hussain Mondal, David Blair, Emdadul Haque Chowdhury, M. Dolores Bargues, Santiago Mas-Coma
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  • Potential Hybridization of Fasciola hepatica and F. gigantica in Africa—A Scoping Review
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    Clinical Microbiology Reviews.2022;[Epub]     CrossRef
  • New perspectives for fascioliasis in Upper Egypt’s new endemic region: Sociodemographic characteristics and phylogenetic analysis of Fasciola in humans, animals, and lymnaeid vectors
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    PLOS Neglected Tropical Diseases.2022; 16(12): e0011000.     CrossRef
  • Morphological and molecular characterization of Fasciola hepatica and Fasciola gigantica phenotypes from co-endemic localities in Mpumalanga and KwaZulu-Natal provinces of South Africa
    Sayurika Haridwal, Mokgadi P. Malatji, Samson Mukaratirwa
    Food and Waterborne Parasitology.2021; 22: e00114.     CrossRef
  • Invasive snails, parasite spillback, and potential parasite spillover drive parasitic diseases of Hippopotamus amphibius in artificial lakes of Zimbabwe
    Ruben Schols, Hans Carolus, Cyril Hammoud, Kudzai C. Muzarabani, Maxwell Barson, Tine Huyse
    BMC Biology.2021;[Epub]     CrossRef
  • Description and phylogenetic analyses of ribosomal transcription units from species of Fasciolidae (Platyhelminthes: Digenea)
    T.H. Le, K.L.T. Pham, H.T.T. Doan, T.K. Xuyen Le, K.T. Nguyen, S.P. Lawton
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Exposing the Barcoding Void: An Integrative Approach to Study Snail-Borne Parasites in a One Health Context
    Ruben Schols, Aspire Mudavanhu, Hans Carolus, Cyril Hammoud, Kudzai C. Muzarabani, Maxwell Barson, Tine Huyse
    Frontiers in Veterinary Science.2020;[Epub]     CrossRef
  • Identification of Adult Fasciola spp. Using Matrix-Assisted Laser/Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry
    Issa Sy, Lena Margardt, Emmanuel O. Ngbede, Mohammed I. Adah, Saheed T. Yusuf, Jennifer Keiser, Jacqueline Rehner, Jürg Utzinger, Sven Poppert, Sören L. Becker
    Microorganisms.2020; 9(1): 82.     CrossRef
  • 11,657 View
  • 200 Download
  • 25 Web of Science
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Prevalence of Soil-Transmitted Helminths and Molecular Clarification of Hookworm Species in Ethnic Ede Primary Schoolchildren in Dak Lak Province, Southern Vietnam
Bui Khac Hung, Nguyen Van De, Le Van Duyet, Jong-Yil Chai
Korean J Parasitol 2016;54(4):471-476.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.471
To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25.0%, including 2.0% Ascaris lumbricoides, 0.33% Trichuris trichiura, and 22.8% hookworm infections. The average intensity of infection was 102.0 eggs per gram of feces (EPG) for Ascaris, 36.0 EPG for Trichuris, and 218.0 EPG for hookworms. ITS1 gene sequences of the hookworm larvae were identical with those of Necator americanus (100% homology) reported in GenBank. It has been confirmed in this study that the hookworm, N. americanus, is a dominant helminth species infected in primary schoolchildren of a southern part of Vietnam. Public health attention is needed for control of hookworm infections among schoolchildren in surveyed areas of Vietnam.

Citations

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  • Prevalence and correlates of soil-transmitted helminths in schoolchildren aged 5 to 18 years in low- and middle-income countries: a systematic review and meta-analysis
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  • Epidemiology of soil-transmitted helminths using quantitative PCR and risk factors for hookworm and Necator americanus infection in school children in Dak Lak province, Vietnam
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  • The protective effect of rural life on mite sensitization disappears among urban migrants in the South of Vietnam
    Ha Thi Chu, Thanh Ngoc Tran, Virginie Doyen, Olivier Denis, Thi Thu Thuy Tran, Thi Kieu Diem Nguyen, Huu Lan Nguyen, Minh Xuan Ngo, Thi Mong Hiep Tran, Francis Corazza, Catherine Bouland, Jean-Marie Hauglustaine, Isabelle Godin, Olivier Michel
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  • Evaluation of the Diagnostic Performance of Onchocerca volvulus Linear Epitopes in a Peptide Enzyme-Linked Immunosorbent Assay
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    Sun Huh
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  • 207 Download
  • 12 Web of Science
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Case Report

A Case of Ocular Angiostrongyliasis with Molecular Identification of the Species in Vietnam
Nguyen Van De, Le Van Duyet, Jong-Yil Chai
Korean J Parasitol 2015;53(6):713-717.
Published online December 31, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.6.713
A 23-year-old female residing in a village of Cao Bang Province, North Vietnam, visited the Hospital of Hanoi Medical University in July 2013. She felt dim eyes and a bulge-sticking pain in her left eye for some days before visiting the hospital. In the hospital, a clinical examination, an eye endoscopy, and an operation were carried out. A nematode specimen was collected from the eye of this patient. The body of this worm was thin and long and measured 22.0×0.3 mm. It was morphologically suggested as an immature female worm of Angiostrongylus cantonensis. By a molecular method using 18S rRNA gene, this nematode was confirmed as A. cantonensis. This is the first molecular study for identification of A. cantonensis in Vietnam.

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  • Angiostrongylus cantonensis Is an Important Cause of Eosinophilic Meningitis in Southern Vietnam
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    Clinical Infectious Diseases.2017; 64(12): 1784.     CrossRef
  • Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
    Mu-Xin Chen, Jia-Xu Chen, Shao-Hong Chen, Da-Na Huang, Lin Ai, Ren-Li Zhang
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  • Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails
    Rutchanee Rodpai, Pewpan M. Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Lakkhana Sadaow, Sakhone Laymanivong, Win Papa Aung, Issarapong Phosuk, Porntip Laummaunwai, Wanchai Maleewong, Donald James Colgan
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Mini Review

Current Status of Taeniasis and Cysticercosis in Vietnam
Nguyen Van De, Thanh Hoa Le, Phan Thi Huong Lien, Keeseon S. Eom
Korean J Parasitol 2014;52(2):125-129.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.125

Several reports on taeniasis and cysticercosis in Vietnam show that they are distributed in over 50 of 63 provinces. In some endemic areas, the prevalence of taeniasis was 0.2-12.0% and that of cysticercosis was 1.0-7.2%. The major symptoms of taeniasis included fidgeted anus, proglottids moving out of the anus, and proglottids in the feces. Clinical manifestations of cysticercosis in humans included subcutaneous nodules, epileptic seizures, severe headach, impaired vision, and memory loss. The species identification of Taenia in Vietnam included Taenia asiatica, Taenia saginata, and Taenia solium based on combined morphology and molecular methods. Only T. solium caused cysticercosis in humans. Praziquantel was chosen for treatment of taeniasis and albendazole for treatment of cysticercosis. The infection rate of cysticercus cellulosae in pigs was 0.04% at Hanoi slaughterhouses, 0.03-0.31% at provincial slaughterhouses in the north, and 0.9% in provincial slaughterhouses in the southern region of Vietnam. The infection rate of cysticercus bovis in cattle was 0.03-2.17% at Hanoi slaughterhouses. Risk factors investigated with regard to transmission of Taenia suggested that consumption of raw meat (eating raw meat 4.5-74.3%), inadequate or absent meat inspection and control, poor sanitation in some endemic areas, and use of untreated human waste as a fertilizer for crops may play important roles in Vietnam, although this remains to be validated.

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  • Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools
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  • Seroprevalence of fascioliasis, toxocariasis, strongyloidiasis and cysticercosis in blood samples diagnosed in Medic Medical Center Laboratory, Ho Chi Minh City, Vietnam in 2012
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Case Reports

Molecular Diagnosis of an Ocular Toxocariasis Patient in Vietnam
Nguyen Van De, Nguyen Vu Trung, Le Van Duyet, Jong-Yil Chai
Korean J Parasitol 2013;51(5):563-567.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.563

An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.

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    Amira Dewair, Mohamed Bessat, Jacopo Guccione
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An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam
Nguyen Van De, Nguyen Vu Trung, Nguyen Hong Ha, Vu Thi Nga, Nguyen Minh Ha, Pham Thanh Thuy, Le Van Duyet, Jong-Yil Chai
Korean J Parasitol 2012;50(4):339-343.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.339

The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.

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    Dinh Ng-Nguyen, Mark A. Stevenson, Rebecca J. Traub
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    Thu Huong Nguyen
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Original Article

Foodborne Trematode Metacercariae in Fish from Northern Vietnam and Their Adults Recovered from Experimental Hamsters
Jong-Yil Chai, Nguyen Van De, Woon-Mok Sohn
Korean J Parasitol 2012;50(4):317-325.
Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.317

The prevalence of foodborne trematode (FBT) metacercariae was investigated in fish from 2 localities of northern Vietnam in 2004-2005. Freshwater fish (9 species) were collected from local markets in Hanoi City (n=76) and Nam Dinh Province (n=79), and were examined for FBT metacercariae using the artificial digestion technique. Adult flukes were obtained from hamsters experimentally infected with the metacercariae at day 8 post-infection. Three (Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium) and 6 (Haplorchis taichui, H. pumilio, C. formosanus, P. varium, Stellantchasmus falcatus, and Heterophyopsis continua) species of FBT metacercariae were detected in the 2 regions, respectively. Overall, among the positive fish species, H. pumilio metacercariae were detected in 104 (80.0%) of 130 fish examined (metacercarial density per infected fish; 64.2). C. formosanus metacercariae were found in 37 (40.2%) of 92 fish (metacercarial density; 14.7). P. varium metacercariae were detected in 19 (63.3%) of 30 fish (Anabas testudineus and Mugil cephalus) (metacercarial density; 247.7). S. falcatus metacercariae were found in all 10 M. cephalus examined (metacercarial density; 84.4). H. continua metacercariae (2 in number) were detected in 1 fish of Coilia lindmani. Morphologic characteristics of the FBT metacercariae and their experimentally obtained adults were described. The results have demonstrated that various FBT species are prevalent in northen parts of Vietnam.

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Case Reports

The First Human Case of Thelazia callipaeda Infection in Vietnam
Nguyen Van De, Thanh Hoa Le, Jong-Yil Chai
Korean J Parasitol 2012;50(3):221-223.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.221

A 26-year-old man residing in a village of Thai Nguyen Province, North Vietnam, visited the Thai Nguyen Provincial Hospital in July 2008. He felt a bulge-sticking pain in his left eye and extracted 5 small nematode worms by himself half a day before visiting the hospital. Two more worms were extracted from his left eye by a medical doctor, and they were morphologically observed and genetically analyzed on the mitochondrial cytochrome c oxidase 1 gene. The worms were 1 male and 1 female, and genetically identical with those of Thelazia callipaeda. By the present study, the presence of human T. callipaeda infection is first reported in Vietnam.

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From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.

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Brief Communication
Echinostoma revolutum: Metacercariae in Filopaludina Snails from Nam Dinh Province, Vietnam, and Adults from Experimental Hamsters
Jong-Yil Chai, Woon-Mok Sohn, Byoung-Kuk Na, Nguyen Van De
Korean J Parasitol 2011;49(4):449-455.
Published online December 16, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.4.449

We detected metacercariae of Echinostoma revolutum in Filopaludina sp. snails purchased from a local market in Nam Dinh Province for the first time in Vietnam. Adult flukes were harvested from experimentally infected hamsters at days 14 and 17 post-infection. The metacercariae were round, 170-190 ?m (n=15) in diameter, with a cyst wall thickness of about 12 ?m. A total of 37 collar spines were arranged around the head collar, and large excretory granules were seen in 2 canals of the excretory bladder. The 14-day old adult flukes were elongated, ventrally curved, and 5.0-7.2×0.8-1.3 mm (n=20). The head collar had a total of 37 collar spines arranged in 2 alternating rows, including 5 corner spines on each side. The cirrus sac contained a saccular seminal vesicle, a prostatic gland, and an unarmed cirrus. Two tandem testes were smooth or slightly lobed. Eggs were ovoid to elliptical, 110-118×70-75 ?m. These morphological characters were similar to those of E. revolutum and E. jurini. We tentatively identified it as E. revolutum because the validity of E. jurini remains to be elucidated. The taxonomic relationship of E. revolutum and E. jurini is discussed.

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