Parasites, Hosts and Diseases

"PCR-RFLP"

Case Report

Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea: Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung; Korean J Parasitol 2019;57(4):417-422.
Published online August 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.4.417

Abstract
PDF

From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

Citations

Citations to this article as recorded by

Exploring bioactive molecules released during inter- and intraspecific competition: A paradigm for novel antiparasitic drug discovery and design for human use
Pichet Ruenchit
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100256. CrossRef
Taeniasis impacts human gut microbiome composition and function
Wenjie Mu, Pingping Ma, Yugui Wang, Yaqi Li, Yingying Ding, Yang Zou, Lixia Pu, Qi Yan, Haoyue Kong, Xiaola Guo, Aijiang Guo, Hailong Li, Shuai Wang
The ISME Journal.2024;[Epub] CrossRef
Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China
Xiu-Min Han, Xue-Yong Zhang, Ying-Na Jian, Qing-Shan Tian
The Korean Journal of Parasitology.2021; 59(3): 311. CrossRef

11,892 View
239 Download
4 Web of Science
Crossref

Original Articles

First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal: Haroon Ahmed, S?rgio Ramalho Sousa, Sami Simsek, Sofia Anast?cio, Seyma Gunyakti Kilinc; Korean J Parasitol 2017;55(6):653-658.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.653

Abstract
PDF

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The
objective
of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Citations

Citations to this article as recorded by

Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia
Ema Gagović, Daria Jurković Žilić, Krunoslav Pintur, Adnan Hodžić, Šimun Naletilić, Relja Beck
Animals.2025; 15(2): 208. CrossRef
Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain)
Sara González, Rosario Panadero, María Luisa Del Rio, María Natividad Díez, María del Rosario Hidalgo, Angélica Martínez
Veterinary Research Communications.2024; 48(2): 761. CrossRef
Molecular characterization of the camel nasal botfly, Cephalopina titillator (Diptera: Oestridae)
Mona G. Shaalan, Sherif Hamed Farghaly, Emad I. Khater, Mohamed A. Kenawy, Enas Hamdy Ghallab
Beni-Suef University Journal of Basic and Applied Sciences.2024;[Epub] CrossRef
Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis
Sara González, Maria Luisa Del Rio, Maria Natividad Diez, Maria del Rosario Hidalgo, Angelica Martínez
Microscopy Research and Technique.2023; 86(1): 3. CrossRef
Temporal and spatial spread of Hypoderma actaeon infection in roe deer from peninsular Spain determined by an indirect enzyme‐linked immunosorbent assay
R. Panadero, C. M. López, S. Remesar, E. Cabanelas, G. Varas, F. Markina, P. Díaz, D. García‐Dios, A. Prieto, G. Fernández, P. Díez‐Baños, P. Morrondo
Medical and Veterinary Entomology.2020; 34(1): 44. CrossRef

8,669 View
155 Download
8 Web of Science
Crossref

Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis: Sara Nemati, Asghar Fazaeli, Homa Hajjaran, Ali Khamesipour, Mohsen Falahati Anbaran, Arezoo Bozorgomid, Fatah Zarei; Korean J Parasitol 2017;55(4):367-374.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.367

Abstract
PDF

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.

Citations

Citations to this article as recorded by

Existing and newly emerging human cutaneous Leishmania isolates in Ethiopia: a systematic review
Abiy Ayele Angelo, Getu Girmay, Dereje Mengesha Berta, Bisrat Birke Teketelew, Elias Chane, Negesse Cherie, Muluneh Assefa, Zufan Yiheyis Abriham, Mebratu Tamir
Discover Medicine.2025;[Epub] CrossRef
Update on the Seroepidemiology of Human Cystic Echinococcosis and Associated Risk Factors in Iran: A Systematic Review and Meta‐Analysis
Nashmin Mohemmi, Mohammad Taghi Khodayari, Seyed Ahmad Karamati, Mohammad Reza Shiee, Hossein Effatpanah, Afshin Davari, Ali Reza Ghorbani, Arezoo Bozorgomid
Health Science Reports.2025;[Epub] CrossRef
Genetic Diversity and Chromosomal Variations in the Iranian Leishmania major Strain: Insights into Pathogenicity and Drug Resistance
Hanieh Sharifian, Anis Khalafiyan, Mahmood Fadaie, Hossein Khanahmad, Zabihollah Shahmoradi, Erfan Zaker, Parisa Mousavi, Nadia Pourmoshir, Azadeh Zolfaghari
Advanced Biomedical Research.2025;[Epub] CrossRef
Genetic diversity and epidemiological insights into cutaneous leishmaniasis in Pakistan: a comprehensive study on clinical manifestations and molecular characterization of Leishmania species
Shumaila Naz, Muhammed Nalcaci, Obaid Hayat, Seray Toz, Azhar Minhas, Shahid Waseem, Yusuf Ozbel
Parasitology Research.2024;[Epub] CrossRef
Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran
Naser Nazari, Mohamad Bagher Rokni, Madoka Ichikawa‐Seki, Saber Raeghi, Homa Hajjaran, Shahab Falahi, Yazdan Hamzavi, Peyman Heydarian, Afshin Davari, Keyphobad Ghadiri, Arezoo Bozorgomid
Veterinary Medicine and Science.2023; 9(2): 924. CrossRef
Real‐time impact of COVID‐19 pandemic on cutaneous leishmaniasis case finding and strategic planning, preventive interventions, control and epidemiology in a region with a high burden of cutaneous leishmaniasis and COVID‐19: A cross‐sectional descriptive
Morteza Shams, Ayoub Rashidi, Jasem Mohamadi, Mohamad Moradi, Reza Pakzad, Razi Naserifar, Jahangir Abdi, Fariba Ghelichi, Arezoo Bozorgomid, Nahid Maspi, Azra Kenarkoohi, Yasin Mohammadi, Amir Abdoli, Shahab Falahi
Health Science Reports.2023;[Epub] CrossRef
An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia
Adriana C. Castillo-Castañeda, Luz H. Patiño, Maria Fernanda Zuñiga, Omar Cantillo-Barraza, Martha S. Ayala, Maryi Segura, Jessica Bautista, Plutarco Urbano, Jeiczon Jaimes-Dueñez, Juan David Ramírez
Parasites & Vectors.2022;[Epub] CrossRef
Taxonomy, Population Structure and Genetic Diversity of Iranian Leishmania Strains of Cutaneous and Visceral Leishmaniasis
Sara Nemati, Homa Hajjaran, Soudabeh Heydari, Asghar Fazaeli, Ali Khamesipour, Mohsen Falahati Anbaran, Mehdi Mohebali, Hamed Mirjalali
Acta Parasitologica.2021; 66(4): 1274. CrossRef
The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis
Homa Hajjaran, Reza Saberi, Alireza Borjian, Mahdi Fakhar, Seyed Abdollah Hosseini, Sajjad Ghodrati, Mehdi Mohebali
Frontiers in Public Health.2021;[Epub] CrossRef
Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania
Juan Jose Lauthier, Paula Ruybal, Paola Andrea Barroso, Yoshihisa Hashiguchi, Jorge Diego Marco, Masataka Korenaga
Acta Tropica.2020; 201: 105189. CrossRef
Isolation and Molecular Identification of Leishmania spp. in Patients With Cutaneous Leishmaniasis in Golestan Province, Iran
Ali Fattahi Bafghi, Gilda Eslami, Oghol Niazjorjani, Farzaneh Mirzaei, Javad Namrodi
International Journal of Epidemiologic Research.2019; 6(1): 8. CrossRef
Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks
Giovanny Herrera, Adriana Higuera, Luz Helena Patiño, Martha S. Ayala, Juan David Ramírez
Infection, Genetics and Evolution.2018; 64: 135. CrossRef
Cutaneous leishmaniasis in Qasr-e Shirin, a border area in the west of Iran
Yazdan Hamzavi, Naser Nazari, Nahid Khademi, Keivan Hassani, Arezoo Bozorgomid
Veterinary World.2018; : 1692. CrossRef

10,807 View
234 Download
13 Web of Science
Crossref

Isolation and Genotyping of Toxoplasma gondii Strains in Ovine Aborted Fetuses in Khorasan Razavi Province, Iran: Leila Danehchin, Gholamreza Razmi, Abolghasem Naghibi; Korean J Parasitol 2016;54(1):15-20.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.15

Abstract
PDF

Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxolasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged 7-22 ?m. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.

Citations

Citations to this article as recorded by

Unraveling the link: serological and molecular insights into Toxoplasma gondii infection in women with spontaneous abortion history
Narges Arbabi, Nima Firouzeh, Seyed Ghader Azizi, Ahmad Mehravaran, Soudabeh Etemadi, Reza Shafiei, Hadi Mirahmadi
Tropical Diseases, Travel Medicine and Vaccines.2025;[Epub] CrossRef
Is GRA6 Gene a Suitable Marker for Molecular Typing of Toxoplasma Gondii? A Scoping Systematic Review
Rohallah Abedian, Bahman Rahimi Esboei, Mahdi Fakhar, Zahra Rahmani, Mahbobeh Montazeri, Mostafa Solaymani, Hajar Ziaei Hezarjaribi
Acta Parasitologica.2024; 69(1): 175. CrossRef
Serological and molecular survey of Toxoplasma Gondii in aborted livestock fetuses from Northeast Iran
Reza Shafiei, Nima Firouzeh, Mohammad Taghi Rahimi
BMC Research Notes.2024;[Epub] CrossRef
Toxoplasmosis diagnostic techniques: Current developed methods and biosensors
Soheila Molaei, Masoomeh Dadkhah, Farzaneh Fathi
Talanta.2023; 252: 123828. CrossRef
Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran
Shiva Zeinali, Shahram Khademvatan, Rasool Jafari, Shabnam Vazifekhah, Elham Yousefi, Tahereh Behroozi-Lak, Bibi Razieh Hosseini Farash
PLOS ONE.2023; 18(10): e0283493. CrossRef
Investigation of Toxoplasma gondii and Neospora caninum as cause of ovine abortion in affected flocks of Urmia, northwest of Iran
A. Khodadadi, F. Malekifard, R. A. Batavani
BULGARIAN JOURNAL OF VETERINARY MEDICINE.2022; 25(2): 308. CrossRef
Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers
Amin Yousefvand, Seyed Ali Mirhosseini, Masoud Ghorbani, Tahereh Mohammadzadeh, Mehrdad Moosazadeh Moghaddam, Shirin Mohammadyari
Journal of Consumer Protection and Food Safety.2021; 16(2): 117. CrossRef
Global prevalence of Toxoplasma gondii infection in the aborted fetuses and ruminants that had an abortion: A systematic review and meta-analysis
Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Ahmad Daryani
Veterinary Parasitology.2021; 290: 109370. CrossRef
Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh
Tanjila Hasan, Abdul Mannan, Delower Hossain, Azizunnesa Rekha, Md. Monir Hossan, Mohammad Abdul Alim, AHM Musleh Uddin
Veterinary World.2021; : 2386. CrossRef
Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran–Iraq
Arjang Partoandazanpoor, Zainab Sadeghi-Dehkordi, Loghman Ekradi, Monireh Khordadmehr, Maryam Rassouli, Alireza Sazmand
Acta Parasitologica.2020; 65(1): 187. CrossRef
WITHDRAWN: Economic and public health importance of Toxoplasma gondii infections in sheep: the last decade
J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su
Veterinary Parasitology: X.2020; : 100028. CrossRef
Economic and public health importance of Toxoplasma gondii infections in sheep: 2009–2020
J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su
Veterinary Parasitology.2020; 286: 109195. CrossRef
Lack of circulating Toxoplasma gondii DNA in seropositive patients with bipolar or schizophrenia spectrum disorders.
L. Galli, C. Del Grande, L. Rindi, C. Mangia, V. Mangano, E. Schiavi, I. Masci, B. Pinto, L. Kramer, L. Dell'Osso, F. Bruschi
Psychiatry Research.2019;[Epub] CrossRef
Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact
S. Stelzer, W. Basso, J. Benavides Silván, L.M. Ortega-Mora, P. Maksimov, J. Gethmann, F.J. Conraths, G. Schares
Food and Waterborne Parasitology.2019; 15: e00037. CrossRef
Detection of Toxoplasma gondii genotypes in abortion women by RFLP-PCR in Al-Najaf Al-Ashraf province
Saleem Khteer Al-Hadraawy, Mohammad Alzeyadi, Ameer Ali Shakir, Arshad noori Al-dujaili
Journal of Physics: Conference Series.2019; 1234(1): 012084. CrossRef
Molecular genotyping and serological evaluation of Toxoplasma gondii in mothers and their spontaneous aborted fetuses in Southwest of Iran
Nasir Arefkhah, Bahman Pourabbas, Qasem Asgari, Abdolali Moshfe, Fataneh Mikaeili, Gordafarin Nikbakht, Bahador Sarkari
Comparative Immunology, Microbiology and Infectious Diseases.2019; 66: 101342. CrossRef
Serological and molecular detection ofToxoplasma gondiiin sheep and goats in Kashan, Central Iran
Sima Rasti, Nader Marandi, Amir Abdoli, Mahdi Delavari, Seyed Gholam Abbas Mousavi
Journal of Food Safety.2018;[Epub] CrossRef
Prevalence and molecular characterization of Toxoplasma gondii DNA in retail fresh meats in Canada
Asma Iqbal, Nicol Janecko, Frank Pollari, Brent Dixon
Food and Waterborne Parasitology.2018; 13: e00031. CrossRef
Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran
Belal Armand, Kavous Solhjoo, Manoochehr Shabani Kordshooli, Mohammad Hasan Davami, Morteza Pourahmad, Vahideh Orfaee
Veterinary World.2017; 10(4): 386. CrossRef
Seroprevalence and genetic characterization of Toxoplasma gondii in masked palm civet ( Paguma larvata ) in Hainan province, tropical China
Guan-Yu Hou, Jun-Ming Zhao, Han-Lin Zhou, Guang Rong
Acta Tropica.2016; 162: 103. CrossRef

11,132 View
149 Download
29 Web of Science
Crossref

An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting: Yoursry Hawash, M. M. Ghonaim, S. S. Al-Shehri; Korean J Parasitol 2016;54(1):1-8.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.1

Abstract
PDF

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, RIDA^® Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

Citations

Citations to this article as recorded by

A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates
Ajanta Ghosal, Sanjib K. Sardar, Tapas Haldar, Akash Prasad, Koushik Das, Seiki Kobayashi, Yumiko Saito-Nakano, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly
Diagnostic Microbiology and Infectious Disease.2024; 110(4): 116548. CrossRef
Comparison of ELISA, nested PCR and sequencing and a novel qPCR for detection of Giardia isolates from Jordan
Nawal Hijjawi, Rongchang Yang, Ma'mon Hatmal, Yasmeen Yassin, Taghrid Mharib, Rami Mukbel, Sameer Alhaj Mahmoud, Abdel-Ellah Al-Shudifat, Una Ryan
Experimental Parasitology.2018; 185: 23. CrossRef
Methods for the detection of Cryptosporidium and Giardia: From microscopy to nucleic acid based tools in clinical and environmental regimes
Folasade Esther Adeyemo, Gulshan Singh, Poovendhree Reddy, Thor Axel Stenström
Acta Tropica.2018; 184: 15. CrossRef
High Frequency of Enteric Protozoan, Viral, and Bacterial Potential Pathogens in Community-Acquired Acute Diarrheal Episodes: Evidence Based on Results of Luminex Gastrointestinal Pathogen Panel Assay
Yousry A. Hawash, Khadiga A. Ismail, Mazen Almehmadi
The Korean Journal of Parasitology.2017; 55(5): 513. CrossRef

11,467 View
167 Download
7 Web of Science
Crossref

Brief Communication

Anisakis pegreffii Larvae in Sea Eels (Astroconger myriaster) from the South Sea, Republic of Korea: Jaeeun Cho, Hyemi Lim, Bong-Kwang Jung, Eun-Hee Shin, Jong-Yil Chai; Korean J Parasitol 2015;53(3):349-353.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.349

Abstract
PDF

Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.

Citations

Citations to this article as recorded by

Anisakidae and Anisakidosis: A Public Health Perspective
Diana Nonković, Vanja Tešić, Vida Šimat, Svjetlana Karabuva, Alan Medić, Jerko Hrabar
Pathogens.2025; 14(3): 217. CrossRef
Rapid and Ultrasensitive Detection of H. aduncum via the RPA-CRISPR/Cas12a Platform
Xiaoming Wang, Xiang Chen, Ting Xu, Xingsheng Jin, Junfang Jiang, Feng Guan
Molecules.2024; 29(20): 4789. CrossRef
Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters
Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn
Parasitology Research.2024;[Epub] CrossRef
Prevalence of Anisakid Nematodes in Fish in China: A Systematic Review and Meta-Analysis
Qing Liu, Qi Wang, Jing Jiang, Jun-Yang Ma, Xing-Quan Zhu, Qing-Long Gong
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Demographic history and population genetic structure of Anisakis pegreffii in the cutlassfish Trichiurus japonicus along the coast of mainland China and Taiwan
Fang Ding, Sui Gu, Mu-Rong Yi, Yun-Rong Yan, Wei-Kuang Wang, Kwong-Chung Tung
Parasitology Research.2022; 121(10): 2803. CrossRef
First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
Hoang Van Hien, Bui Thi Dung, Ha Duy Ngo, Pham Ngoc Doanh
Journal of Nematology.2021; 53(1): 1. CrossRef
Comparison of risk factors between human intestinal and gastric Anisakiasis
Kazuki Yamamoto, Osamu Takahashi, Daiki Kobayashi
Parasitology International.2020; 75: 102024. CrossRef
Genera and Species of the Anisakidae Family and Their Geographical Distribution
Juan C. Ángeles-Hernández, Fabian R. Gómez-de Anda, Nydia E. Reyes-Rodríguez, Vicente Vega-Sánchez, Patricia B. García-Reyna, Rafael G. Campos-Montiel, Norma L. Calderón-Apodaca, Celene Salgado-Miranda, Andrea P. Zepeda-Velázquez
Animals.2020; 10(12): 2374. CrossRef
Establishment and validation of ARMS (amplification-refractory mutation system) for identification of Anisakis species collected from Korean waters
Hyunsu Kim, Kyung-Wan Baek, Mi-Kyung Park, Kyung-Yoon Jeon, Eun-Ji Ko, Hee-Jae Cha, Mee Sun Ock
Gene.2019; 691: 125. CrossRef
Molecular Identification of Anisakis Larvae Extracted by Gastrointestinal Endoscopy from Health Check-up Patients in Korea
Hyemi Song, Bong-Kwang Jung, Jaeeun Cho, Taehee Chang, Sun Huh, Jong-Yil Chai
The Korean Journal of Parasitology.2019; 57(2): 207. CrossRef
Anisakid Larvae from Anchovies in the South Coast of Korea
Taehee Chang, Bong-Kwang Jung, Sooji Hong, Hyejoo Shin, Jeonggyu Lee, Laddawan Patarwut, Jong-Yil Chai
The Korean Journal of Parasitology.2019; 57(6): 699. CrossRef
Anisakiasis Causing Acute Dysentery in Malaysia
Amirah Amir, Romano Ngui, Yee-Ling Lau, Wan Hafiz Wan Ismail, Rohela Mahmud, Kum T. Wong, Jaxinthe S. K. Ong, Yvonne A. L. Lim
The American Journal of Tropical Medicine and Hygiene.2016; 95(2): 410. CrossRef

10,570 View
119 Download
13 Web of Science
Crossref

Original Articles

Genetic Diversity of Schistosoma haematobium Eggs Isolated from Human Urine in Sudan: Juan-Hua Quan, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Abdoelohab Saed Mohamed, Hoo-Gn Jeong, Jin-Su Lee, Sung-Tae Hong, Tai-Soon Yong, Guang-Ho Cha, Young-Ha Lee; Korean J Parasitol 2015;53(3):271-277.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.271

Abstract
PDF

The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.

Citations

Citations to this article as recorded by

Analysis of DNA cox1 barcoding revealed novel haplotype in Schistosoma haematobium isolated from Western Sudan
Ishraga Adam Elzain, Abeer Babiker Idris, Abdul Aziz Karim, Nagla Mohamed Ahmed, Salaheldein G. Elzaki, Semih Yılmaz, Mohamed A. Hassan, Hamid Suliman Abdalla
Scientific Reports.2025;[Epub] CrossRef
Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon
Lady Charlène Kouna, Sandrine Lydie Oyegue-Liabagui, Chenis Nick Atiga, Chérone Nancy Mbani Mpega Ntigui, Roméo Karl Imboumy-Limoukou, Jean Claude Biteghe BI Essone, Steede Seinnat Ontoua, Diamella Nancy Moukodoum, Alain Prince Okouga, Jean Bernard Lekana
Diagnostics.2025; 15(9): 1052. CrossRef
Molecular diagnosis of urogenital schistosomiasis in pre-school children, school-aged children and women of reproductive age at community level in central Senegal
Doudou Sow, Khadime Sylla, Ndeye Marème Dieng, Bruno Senghor, Papa Mouhamadou Gaye, Cheikh B. Fall, Ndiaw Goumballa, Aldiouma Diallo, Jean Louis A. Ndiaye, Philippe Parola, Cheikh Sokhna, Souleymane Doucouré, Babacar Faye
Parasites & Vectors.2023;[Epub] CrossRef
Population genetic structure of Schistosoma haematobium and Schistosoma haematobium × Schistosoma bovis hybrids among school-aged children in Côte d’Ivoire
Etienne K. Angora, Alexane Vangraefschepe, Jean-François Allienne, Hervé Menan, Jean T. Coulibaly, Aboulaye Meïté, Giovanna Raso, Mirko S. Winkler, William Yavo, André O. Touré, Eliézer K. N’Goran, Jakob Zinsstag, Jürg Utzinger, Oliver Balmer, Jérôme Bois
Parasite.2022; 29: 23. CrossRef
Genetic Diversity of Schistosoma haematobium in Sub-Saharan Africa: A Systematic Review
Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S Nalubamba, Chummy S Sikasunge, Andrew M Phri
University of Zambia Journal of Agricultural and Biomedical Sciences.2022;[Epub] CrossRef
Detection of Schistosoma DNA in genital specimens and urine: A comparison between five female African study populations originating from S. haematobium and/or S. mansoni endemic areas
P. Pillay, J.A. Downs, J.M. Changalucha, E.A.T. Brienen, C.E. Ramarokoto, P.D.C. Leutscher, B.J. Vennervald, M. Taylor, E.F. Kjetland, L. Van Lieshout
Acta Tropica.2020; 204: 105363. CrossRef

11,745 View
207 Download
8 Web of Science
Crossref

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions: Shuai Wang, Aiping Cao, Xun Li, Qunli Zhao, Yuan Liu, Hua Cong, Shenyi He, Huaiyu Zhou; Korean J Parasitol 2015;53(3):253-258.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.253

Abstract
PDF

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

Citations

Citations to this article as recorded by

Superoxide Dismutases in Immune Regulation and Infectious Diseases
Tong Liu, Jiajin Shang, Qijun Chen
Antioxidants.2025; 14(7): 809. CrossRef
Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
BMC Infectious Diseases.2017;[Epub] CrossRef

10,328 View
102 Download
2 Web of Science
Crossref

Brief Communication

Genetic Polymorphisms in Plasmodium vivax Dihydrofolate Reductase and Dihydropteroate Synthase in Isolates from the Philippines, Bangladesh, and Nepal: Pimwan Thongdee, Jiraporn Kuesap, Kanchana Rungsihirunrat, Shyam Prakash Dumre, Effie Espino, Harald Noedl, Kesara Na-Bangchang; Korean J Parasitol 2015;53(2):227-232.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.227

Abstract
PDF

Genetic polymorphisms of pvdhfr and pvdhps genes of Plasmodium vivax were investigated in 83 blood samples collected from patients in the Philippines, Bangladesh, and Nepal. The SNP-haplotypes of the pvdhfr gene at the amino acid positions 13, 33, 57, 58, 61, 117, and 173, and that of the pvdhps gene at the positions 383 and 553 were analyzed by nested PCR-RFLP. Results suggest diverse polymorphic patterns of pvdhfr alone as well as the combination patterns with pvdhps mutant alleles in P. vivax isolates collected from the 3 endemic countries in Asia. All samples carried mutant combination alleles of pvdhfr and pvdhps. The most prevalent combination alleles found in samples from the Philippines and Bangladesh were triple mutant pvdhfr combined with single mutant pvdhps allele and triple mutant pvdhfr combined with double wild-type pvdhps alleles, respectively. Those collected from Nepal were quadruple mutant pvdhfr combined with double wild-type pvdhps alleles. New alternative antifolate drugs which are effective against sulfadoxine-pyrimethamine (SP)-resistant P. vivax are required.

Citations

Citations to this article as recorded by

Molecular surveillance of antifolate drug resistance markers in Plasmodium vivax from Khyber Pakhtunkhwa province, northwest Pakistan
Thu Hằng Nguyễn, Hương Giang Lê, Tuấn Cường Võ, Đăng Thùy Dương Nguyễn, Kim Oanh Nguyễn, Minkyoung Cho, Youn-Kyoung Goo, Sahib Gul Afridi, Byoung-Kuk Na
Acta Tropica.2025; 264: 107583. CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand
Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin
Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Geographical spread and structural basis of sulfadoxine-pyrimethamine drug-resistant malaria parasites
Rini Chaturvedi, Jyoti Chhibber-Goel, Ishika Verma, Sreehari Gopinathan, Suhel Parvez, Amit Sharma
International Journal for Parasitology.2021; 51(7): 505. CrossRef
Molecular monitoring of dihydrofolatereductase (dhfr) and dihydropteroatesynthetase (dhps) associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates of Palawan, Philippines
Alison Paolo Bareng, Fe Esperanza Espino, Wanna Chaijaroenkul, Kesara Na-Bangchang
Acta Tropica.2018; 180: 81. CrossRef
Mutations of pvdhfr and pvdhps genes in vivax endemic-malaria areas in Kota Marudu and Kalabakan, Sabah
Umi Rubiah Sastu, Noor Rain Abdullah, Nor Azrina Norahmad, Muhammad Nor Farhan Saat, Prem Kumar Muniandy, Jenarun Jelip, Moizin Tikuson, Norsalleh Yusof, Hasidah Mohd Sidek
Malaria Journal.2016;[Epub] CrossRef
Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia
Naowarat Saralamba, Supatchara Nakeesathit, Mayfong Mayxay, Paul N. Newton, Lyda Osorio, Jung-Ryong Kim, Nicholas J. White, Nicholas P. J. Day, Arjen M. Dondorp, Mallika Imwong
Malaria Journal.2016;[Epub] CrossRef

9,191 View
111 Download
6 Web of Science
Crossref

Original Articles

Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea: Woon-Mok Sohn, Jung-Mi Kang, Byoung-Kuk Na; Korean J Parasitol 2014;52(4):383-389.
Published online August 29, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.4.383

Abstract
PDF

Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.

Citations

Citations to this article as recorded by

Comparative study of Taqman-based qPCR assay for the detection of Anisakis simplex and Pseudoterranova decipiens
Mi-Gyeong Kim, Min Ji Hong, Doo Won Seo, Hyun Mi Jung, Hyun-Ja Han, Seung Hwan Kim, Insun Joo, Elingarami Sauli
PLOS One.2025; 20(4): e0320724. CrossRef
Genetic analyses of Anisakis pegreffii (Nematoda: Anisakidae) from the East Asian finless porpoise Neophocaena asiaeorientalis sunameri (Cetacea: Phocoenidae) in Korean waters
Sunmin Kim, Jong Yoon Jeon, Kyunglee Lee, Hyunjoo Lee, Han Chan Park, Kyung Eun Lee, Hang Lee, Sung Bin Lee, Sang Wha Kim, Se Chang Park, Seongjun Choe, Heejeong Youn
Parasitology Research.2024;[Epub] CrossRef
Phylogenetic and phylogeographic analyses of Anisakis simplex sensu stricto (Nematoda: Anisakidae) from the common minke whale in Korean waters
Sunmin Kim, Bom Sok Lee, Seongjun Choe
Parasites, Hosts and Diseases.2023; 61(3): 240. CrossRef
What Do In Vitro and In Vivo Models Tell Us about Anisakiasis? New Tools Still to Be Explored
Serena Cavallero, Ilaria Bellini, Antonella Pizzarelli, Stefano D’Amelio
Pathogens.2022; 11(3): 285. CrossRef
Distribution of Anisakidae Family Infected Marine Fish in Indonesia
Putri Desi Wulan Sari, Sri Subekti, Yarin Dwi Monica
BIOEDUSCIENCE.2021; 5(3): 188. CrossRef
Increasing intensities of Anisakis simplex third-stage larvae (L3) in Atlantic salmon of coastal waters of Scotland
Alexander J. Kent, Campbell C. Pert, Robert A. Briers, Karen Diele, Sonja Rueckert
Parasites & Vectors.2020;[Epub] CrossRef
Occurrence and molecular identification of Anisakis larval type 1 (Nematoda: Anisakidae) in marketed fish in Egypt
Eman Mostafa, Marwa Omar, Shimaa. S. Hassan, Mohamed Samir
Journal of Parasitic Diseases.2020; 44(3): 536. CrossRef
Comparative morphomolecular identification and pathological changes associated with Anisakis simplex larvae (Nematoda: Anisakidae) infecting native and imported chub mackerel (Scomber japonicus) in Egypt
Mohamed Abdelsalam, Marwa M. Attia, Mahmoud A. Mahmoud
Regional Studies in Marine Science.2020; 39: 101469. CrossRef
Establishment and validation of ARMS (amplification-refractory mutation system) for identification of Anisakis species collected from Korean waters
Hyunsu Kim, Kyung-Wan Baek, Mi-Kyung Park, Kyung-Yoon Jeon, Eun-Ji Ko, Hee-Jae Cha, Mee Sun Ock
Gene.2019; 691: 125. CrossRef
Anisakis Nematodes in Fish and Shellfish- from infection to allergies
Ibukun E. Aibinu, Peter M. Smooker, Andreas L. Lopata
International Journal for Parasitology: Parasites and Wildlife.2019; 9: 384. CrossRef
An Overview of Fish-borne Nematodiases among Returned Travelers for Recent 25 Years– Unexpected Diseases Sometimes Far Away from the Origin
Jorge Costa Eiras, Gilberto Cezar Pavanelli, Ricardo Massato Takemoto, Yukifumi Nawa
The Korean Journal of Parasitology.2018; 56(3): 215. CrossRef
Survey for the presence of ascaridoid larvae in the cinnamon flounder Pseudorhombus cinnamoneus (Temminck & Schlegel) (Pleuronectiformes: Paralichthyidae)
Liang Li, Jin-Yu Zhao, Hui-Xia Chen, Hui-Dong Ju, Meng An, Zhen Xu, Lu-Ping Zhang
International Journal of Food Microbiology.2017; 241: 108. CrossRef
Environmental variables and definitive host distribution: a habitat suitability modelling for endohelminth parasites in the marine realm
Thomas Kuhn, Sarah Cunze, Judith Kochmann, Sven Klimpel
Scientific Reports.2016;[Epub] CrossRef
Anisakis pegreffii Larvae in Sea Eels (Astroconger myriaster) from the South Sea, Republic of Korea
Jaeeun Cho, Hyemi Lim, Bong-Kwang Jung, Eun-Hee Shin, Jong-Yil Chai
The Korean Journal of Parasitology.2015; 53(3): 349. CrossRef
Anisakiasis: Report of 15 Gastric Cases Caused by Anisakis Type I Larvae and a Brief Review of Korean Anisakiasis Cases
Woon-Mok Sohn, Byoung-Kuk Na, Tae Hyo Kim, Tae-Joon Park
The Korean Journal of Parasitology.2015; 53(4): 465. CrossRef
Occurrence of Anisakis Larvae in Commercial Fish along the Northern Coast of Taiwan
Yun-Jen Chen, Chun-Chun Wu, Jui-Sen Yang
Research Journal of Parasitology.2015; 10(3): 79. CrossRef

12,042 View
142 Download
13 Web of Science
Crossref

Genotyping of Toxoplasma gondii from Rats (Rattus rattus) in Riyadh, Saudi Arabia: Maha H. Elamin; Korean J Parasitol 2014;52(3):257-261.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.257

Abstract
PDF

Toxoplasma 3 main clonal lineages are designated as type I, II, and III; however, atypical and mixed genotypes were also reported. This study was conducted for detection of Toxoplasma gondii genotypes in rats (Rattus rattus) in Riyadh region, Saudi Arabia. PCR test on T. gondii B1 gene was conducted on ELISA IgM positive samples for confirmation of the infection. However, genetic analysis of the SAG2 locus was performed to determine T. gondii genotypes using PCR-RFLP technique. PCR test on T. gondii B1gene showed that 22 (81.5%) out of the 27 ELISA IgM positive samples have T. gondii DNA. Genotypic analysis shows that, of the total 22 PCR positive samples, only 13 (59.1%) were of type II, 7 (31.8%) were of type III, and 2 (9.1%) were of an unknown genotype. It is obvious that the prevalence of both type II and III is high in rats. No reports have been available on T. gondii genotypes among rats in Riyadh region, and only little is known about its seroprevalence in rats. Future studies on T. gondii genotypes in rats using multi-locus markers is needed in Riyadh region, Saudi Arabia for better understanding of T. gondii pathogenesis and treatment in humans and animals.

Citations

Citations to this article as recorded by

Toxoplasma gondii infection affects the complete blood count and disturbs the markers of oxidative stress from the vital organs of wild rodents
Maryam Ijaz, Asmat Ullah Khan, Shakir Ullah, Afshan Khan, Samir Ibenmoussa, Baye Sitotaw, Turki M. Dawoud, Adil Khan, Furhan Iqbal
Scientific Reports.2024;[Epub] CrossRef
Prevalence of Toxoplasma gondii infection in animals of the Arabian Peninsula between 2000–2020: A systematic review and meta‐analysis
Asmaa Abdelgadier, Nada Assaad, Zaynab Elhussein, Abdulla M. Al‐Marri, Sami Suliman, Khalid Eltom, Ebtisam A. Al‐Mslemani, Abdul Azia Al‐Zeyara, Abdel Rahim M. El Hussein, Khalid A. Enan
Veterinary Medicine and Science.2023; 9(1): 471. CrossRef
The Detection of Toxoplasma gondii in Wild Rats (Rattus norvegicus) on Mink Farms in Shandong Province, Eastern China
Yang Zou, Hong-Li Geng, Hong-Lin Jia, Quan Zhao, Si-Yuan Qin, Xing-Quan Zhu, Xiao-Xuan Zhang
Vector-Borne and Zoonotic Diseases.2022; 22(3): 199. CrossRef
Epidemiological Significance of Toxoplasma gondii Infections in Wild Rodents: 2009–2020
J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cézar, O. C. H. Kwok, C. Su
Journal of Parasitology.2021;[Epub] CrossRef
Molecular Detection and Genotyping of Toxoplasma gondii in Edward's Long-Tailed Rats (Leopoldamys edwardsi)
Wen-Bin Zheng, Bin-Ze Gui, Hai-Bin Long, Yi-Wei Chen, Xing-Quan Zhu, Shui-Lian Wang, Guo-Hua Liu
Foodborne Pathogens and Disease.2019; 16(8): 539. CrossRef

9,156 View
97 Download
9 Web of Science
Crossref

Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia: Hilal Anshary, Sriwulan, Mark A. Freeman, Kazuo Ogawa; Korean J Parasitol 2014;52(1):9-19.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.9

Abstract
PDF

Anisakis spp. (Nematoda: Anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. In this study, 18 fish species were investigated for Anisakis infection. Katsuwonus pelamis, Euthynnus affinis, Caranx sp., and Auxis thazard were infected with high prevalence of Anisakis type I, while Cephalopholis cyanostigma and Rastrelliger kanagurta revealed low prevalence. The mean intensity of Anisakis larvae in K. pelamis and A. thazard was 49.7 and 5.6, respectively. A total of 73 Anisakis type I larvae collected from K. pelamis and A. thazard were all identified as Anisakis typica by PCR-RFLP analysis. Five specimens of Anisakis from K. pelamis and 15 specimens from A. thazard were sequenced using ITS1-5.8S-ITS2 region and 6 specimens from A. thazard and 4 specimens from K. pelamis were sequenced in mtDNA cox2 region. Alignments of the samples in the ITS region showed 2 patterns of nucleotides. The first pattern (genotype) of Anisakis from A. thazard had 100% similarity with adult A. typica from dolphins from USA, whereas the second genotype from A. thazard and K. pelamis had 4 base pairs different in ITS1 region with adult A. typica from USA. In the mtDNA cox2 regions, Anisakis type I specimens from A. thazard and K. pelamis showed similarity range from 94% to 99% with A. typica AB517571/DQ116427. The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait.

Citations

Citations to this article as recorded by

Prevalence Estimates of Anisakis spp. Infection in Fish From Indonesia: A Systematic Review and Meta‐Analysis
Handang Widantara, Dian Meididewi Nuraini, Ekky Ilham Romadhona, Sutanti Sutanti, Morsid Andityas
Journal of Fish Diseases.2025;[Epub] CrossRef
Invasion dynamics and ecological impacts of Anisakis typica in commercial fish from the Western Pacific Ocean
Dhito Dwi Pramardika, Fadjar Satrija, Sulistiono Sulistiono, Risa Tiuria, Arifin Budiman Nugraha
Veterinary World.2025; : 1365. CrossRef
Uncovering the Brittle Star’s Genetic Diversity from Kalimantan and Bali
Nining Nursalim, Eka Maya Kurniasih, Nenik Kholillah, Gabriella T Kurniatami, Rena Galby Andadari, Hilmy Annisa Oktaviana, Rizki Widya Nur Kholifah, Galank Fadqul Janarkho, Angka Mahardini, Ni Kadek Dita Cahyani
IOP Conference Series: Earth and Environmental Science.2025; 1496(1): 012025. CrossRef
Seroprevalence of IgG and IgE Antibodies Against Anisakis in the Presumably Healthy Population of the Canary Islands
Eligia González-Rodríguez, Marta Rodero, J. Alberto Montoya-Alonso, Kevin M. Santana-Hernández, Myriam R. Ventura, Carmen Cuéllar, Eligia Rodríguez-Ponce
Antibodies.2025; 14(3): 60. CrossRef
Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
EFSA Journal.2024;[Epub] CrossRef
Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru
Jhon Darly Chero, Luis Ñacari, Celso Luis Cruces, David Fermín Lopez, Edson Cacique, Ruperto Severino, Jorge Lopez, José Luis Luque, Gloria Saéz
Diversity.2023; 15(7): 820. CrossRef
Potential hazards associated with the consumption of Scombridae fish: Infection and toxicity from raw material and processing
Chiraz Yemmen, Mohamed Gargouri
Journal of Applied Microbiology.2022; 132(6): 4077. CrossRef
Prevalence of Anisakid Nematodes in Fish in China: A Systematic Review and Meta-Analysis
Qing Liu, Qi Wang, Jing Jiang, Jun-Yang Ma, Xing-Quan Zhu, Qing-Long Gong
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Ascaridoid nematodes infecting commercially important marine fish and squid species from Bangladesh waters in the Bay of Bengal
Miguel Bao, Paolo Cipriani, Lucilla Giulietti, Mohammad Ashraful Alam, Marialetizia Palomba, Simonetta Mattiucci, Arne Levsen
Food and Waterborne Parasitology.2022; 27: e00157. CrossRef
Description of Ichthyascaris grandis sp. n., redescription of Raphidascaroides halieutaeae Yin, 1983 and new records of some other raphidascaridid and philometrid nematodes from marine fishes off Java, Indonesia
František Moravec, Thorsten Walter, Asri Trisnani Yuniar
Folia Parasitologica.2022;[Epub] CrossRef
Anisakid nematodes in Trichiurus lepturus and Saurida undosquamis (Teleostea) from the South-West Indian Ocean: Genetic evidence for the existence of sister species within Anisakis typica (s.l.), and food-safety considerations
Paolo Cipriani, Lucilla Giulietti, Salome Daniel Shayo, Julia E. Storesund, Miguel Bao, Marialetizia Palomba, Simonetta Mattiucci, Arne Levsen
Food and Waterborne Parasitology.2022; 28: e00177. CrossRef
Integrative species delimitation and community structure of nematodes in three species of Australian flathead fishes (Scorpaeniformes: Platycephalidae)
Md. Shafaet Hossen, Skye Wassens, Shokoofeh Shamsi
Parasitology Research.2021; 120(2): 461. CrossRef
Morphological profile of L2 Anisakis typica on Indian Mackerel (Rastrelliger kanagurta) from Sedati Fish Auction, Sidoarjo-East Java, Indonesia using Scanning Electron Microscope (SEM)
N Suryani, S Subekti, S Koesdarto, M K Amiin
IOP Conference Series: Earth and Environmental Science.2021; 679(1): 012059. CrossRef
First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae
Alina E. Safonova, Anastasia N. Voronova, Konstantin S. Vainutis
Journal of Nematology.2021; 53(1): 1. CrossRef
Distribution of Anisakidae Family Infected Marine Fish in Indonesia
Putri Desi Wulan Sari, Sri Subekti, Yarin Dwi Monica
BIOEDUSCIENCE.2021; 5(3): 188. CrossRef
Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish
Vipavinee Cheypanya, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat
Asian Pacific Journal of Tropical Medicine.2021; 14(5): 214. CrossRef
Comparative morphomolecular identification and pathological changes associated with Anisakis simplex larvae (Nematoda: Anisakidae) infecting native and imported chub mackerel (Scomber japonicus) in Egypt
Mohamed Abdelsalam, Marwa M. Attia, Mahmoud A. Mahmoud
Regional Studies in Marine Science.2020; 39: 101469. CrossRef
First record of third-stage Terranova larval type II (Nematoda, Anisakidae) in the common ponyfish Leiognathus equulus Forsskål
Nawal Al-Hoshani, Saleh Al-Quraishy, Mohamed A. Dkhil, Ahmed A. Baiomy, Rewaida Abdel-Gaber
Microbial Pathogenesis.2020; 149: 104597. CrossRef
Parasitic Anisakid Nematode Isolated from Stranded Fraser’s Dolphin (Lagenodelphis hosei Fraser, 1956) from Central Philippine Waters
Karl Marx A. Quiazon, Mudjekeewis D. Santos, Tomoyoshi Yoshinaga
The Philippine Journal of Fisheries.2020; : 183. CrossRef
Research Article: Intensity and prevalence of endoparasite helminths in little tuna (Euthynnus affinis) at Muncar and Panarukan Fish Auction Place, East Java, Indonesia
M Pardede, G Mahasri, M. F Ulkhaq
Iranian Journal of Aquatic Animal Health.2020; 6(2): 49. CrossRef
Infection and molecular identification of ascaridoid nematodes from the important marine food fish Japanese threadfin bream Nemipterus japonicus (Bloch) (Perciformes: Nemipteridae) in China
Ning Guo, Hui-Xia Chen, Lu-Ping Zhang, Jia-Yue Zhang, Li-Yang Yang, Liang Li
Infection, Genetics and Evolution.2020; 85: 104562. CrossRef
Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily
Awais Ali Ahmad, Xin Yang, Ting Zhang, Chunqun Wang, Caixian Zhou, Xingrun Yan, Mubashar Hassan, Muhammad Ikram, Min Hu
Genes.2019; 10(2): 107. CrossRef
Characterization of the Complete Mitochondrial Genome of a Whipworm Trichuris skrjabini (Nematoda: Trichuridae)
Awais Ali Ahmad, Muhammad Abu Bakr Shabbir, Yang Xin, Muhammad Ikram, Mian Abdul Hafeez, Chunqun Wang, Ting Zhang, Caixian Zhou, Xingrun Yan, Mubashar Hassan, Min Hu
Genes.2019; 10(6): 438. CrossRef
Infeksi Anisakid pada lumba-lumba hidung botol indo-pasifik (Tursiops aduncus) di situs konservasi lumba-lumba, Indonesia
Ismah Atika Salmah, Risa Tiuria, Agus Setiyono, Tri Isyani Tungga Dewi
ARSHI Veterinary Letters.2018; 2(4): 67. CrossRef
Anisakis (Nematoda: Ascaridoidea) from Indonesia
HW Palm, S Theisen, IM Damriyasa, ES Kusmintarsih, IBM Oka, EA Setyowati, NA Suratma, S Wibowo, S Kleinertz
Diseases of Aquatic Organisms.2017; 123(2): 141. CrossRef
Anisakiasis in Southeast Asia: A story of new tropical disease?
Somsri Wiwanitkit, Viroj Wiwanitkit
Asian Pacific Journal of Tropical Biomedicine.2016; 6(5): 382. CrossRef
Anisakis Dujardin, 1845 infection (Nematoda: Anisakidae) in Pygmy Sperm Whale Kogia breviceps Blainville, 1838 from west Pacific region off the coast of Philippine archipelago
Karl Marx A. Quiazon
Parasitology Research.2016; 115(9): 3663. CrossRef
Environmental variables and definitive host distribution: a habitat suitability modelling for endohelminth parasites in the marine realm
Thomas Kuhn, Sarah Cunze, Judith Kochmann, Sven Klimpel
Scientific Reports.2016;[Epub] CrossRef
POTENSI ALERGI AKIBAT INFEKSI Anisakis typica PADA DAGING IKAN CAKALANG
Lady Cindy Soewarlan
Jurnal Teknologi dan Industri Pangan.2016; 27(2): 200. CrossRef
Occurrence and prevalence of fish-borne Anisakis larvae in the spotted mackerel Scomber australasicus from Taiwanese waters
Hui-Yu Chen, Hsiu-Hui Shih
Acta Tropica.2015; 145: 61. CrossRef

15,146 View
172 Download
27 Web of Science
Crossref

Case Report

Four Cases of Taenia saginata Infection with an Analysis of COX1 Gene: Jaeeun Cho, Bong-Kwang Jung, Hyemi Lim, Min-Jae Kim, Thanapon Yooyen, Dongmin Lee, Keeseon S. Eom, Eun-Hee Shin, Jong-Yil Chai; Korean J Parasitol 2014;52(1):79-83.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.79

Abstract
PDF

Human taeniases had been not uncommon in the Republic of Korea (=Korea) until the 1980s. The prevalence decreased and a national survey in 2004 revealed no Taenia egg positive cases. However, a subsequent national survey in 2012 showed 0.04% (10 cases) prevalence of Taenia spp. eggs suggesting its resurgence in Korea. We recently encountered 4 cases of Taenia saginata infection who had symptoms of taeniasis that included discharge of proglottids. We obtained several proglottids from each case. Because the morphological features of T. saginata are almost indistinguishable from those of Taenia asiatica, molecular analyses using the PCR-RFLP and DNA sequencing of the cytochrome c oxidase subunit 1 (cox1) were performed to identify the species. The PCR-RFLP patterns of all of the 4 specimens were consistent with T. saginata, and the cox1 gene sequence showed 99.8-100% identity with that of T. saginata reported previously from Korea, Japan, China, and Cambodia. All of the 4 patients had the history of travel abroad but its relation with contracting taeniasis was unclear. Our findings may suggest resurgence of T. saginata infection among people in Korea.

Citations

Citations to this article as recorded by

Molecular diagnosis of Taenia saginata from two patients in Palestine: two case reports
Mohammad Asees, Issam Jawabreh, Omar Hamarsheh, Ziad Abdeen, Ayoub Assi, Kifaya Azmi
Journal of Medical Case Reports.2024;[Epub] CrossRef
Molecular Confirmation of Taenia solium Taeniasis in Child, Timor-Leste
Hanna Jin, Sung-Tae Hong, Merita Antonio Armindo Monteiro, Endang da Silva, Odete da Silva Viegas, Felix dos Santos Lopes, Dong Hee Kim, Sung Hye Kim
Emerging Infectious Diseases.2024;[Epub] CrossRef
Taenia saginata Infection Misdiagnosed as Acute Cholecystitis in a Tibetan Patient, in China
Xiu-Min Han, Xue-Yong Zhang, Ying-Na Jian, Qing-Shan Tian
The Korean Journal of Parasitology.2021; 59(3): 311. CrossRef
A co-infection case report of Taenia saginata in a patient with subclinical clonorchiasis confirmed by the combination of diagnostic tools
Ju Hyeon Shin, Eun Jeong Won, Jee Seung Jung, Kyung-Hwa Park, Kwang Il Nam, Soo Hyun Kim, Jong Hee Shin
BMC Infectious Diseases.2019;[Epub] CrossRef
Neurocysticercosis: Clinical Characteristics and Changes from 26 Years of Experience in an University Hospital in Korea
Hyo-Ju Son, Min Jae Kim, Kyung Hwa Jung, Sungim Choi, Jiwon Jung, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Bong-Kwang Jung, Hyemi Song, Jong-Yil Chai
The Korean Journal of Parasitology.2019; 57(3): 265. CrossRef
Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea
Eun Jeong Won, Ju Hyeon Shin, Yu Jeong Lee, Moon-Ju Kim, Seung Ji Kang, Sook In Jung, Soo Hyun Kim, Jong Hee Shin, Jong-Yil Chai, Sung-Shik Shin
The Korean Journal of Parasitology.2019; 57(3): 313. CrossRef
Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea
Su-Min Song, Hae Soo Yun, Dorene VanBik, Hyun-Ha Chang, Sang-Ah Lee, Shin-Woo Kim, Namhee Ryoo, Dong Yeub Eun, Nan Young Lee, Youn-Kyoung Goo, Yeonchul Hong, Meesun Ock, Hee-Jae Cha, Dong-Il Chung
The Korean Journal of Parasitology.2019; 57(4): 417. CrossRef
Prevalence of cysticercosis in Estonian pigs and cattle
Elisabeth Dorbek-Kolin, Tuuli Åhlberg, Lea Tummeleht, Dennis Tappe, Maria Vang Johansen, Brian Lassen
Parasitology Research.2018; 117(2): 591. CrossRef
Molecular Diagnosis of Taenia saginata Tapeworm Infection in 2 Schoolchildren, Myanmar
Eun Jeong Won, Bong-Kwang Jung, Hyemi Song, Mi-Seon Kim, Hyun-Seung Kim, Keon Hoon Lee, Min-Jae Kim, Myung Geun Shin, Jong Hee Shin, Soon-Pal Suh, Sung-Jong Hong, Woon-Mok Sohn, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai
Emerging Infectious Diseases.2018; 24(6): 1156. CrossRef
Genetic Characterization ofTaenia saginataCyst Isolates from Germany
Sameh Abuseir, Sabine Schicht, Andrea Springer, Uschi Nagel-Kohl, Christina Strube
Vector-Borne and Zoonotic Diseases.2018; 18(8): 433. CrossRef
Cysticercosis/taeniasis endemicity in Southeast Asia: Current status and control measures
Hai-Wei Wu, Akira Ito, Lin Ai, Xiao-Nong Zhou, Luz P. Acosta, Arve Lee Willingham III
Acta Tropica.2017; 165: 121. CrossRef
Molecular identification of cestodes and nematodes by cox1 gene real-time PCR and sequencing
Rosana W.S. Poon, Emily W.T. Tam, Susanna K.P. Lau, Vincent C.C. Cheng, Kwok-Yung Yuen, Rolf K. Schuster, Patrick C.Y. Woo
Diagnostic Microbiology and Infectious Disease.2017; 89(3): 185. CrossRef

12,039 View
110 Download
13 Web of Science
Crossref

Brief Communications

Prevalence and Genetic Characterization of Toxoplasma gondii in House Sparrows (Passer domesticus) in Lanzhou, China: Wei Cong, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Nian-Zhang Zhang, Quan Zhao, Xing-Quan Zhu; Korean J Parasitol 2013;51(3):363-367.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.363

Abstract
PDF

The prevalence of Toxoplasma gondii infection in birds has epidemiological significance because birds are indeed considered as a good indicator of environmental contamination by T. gondii oocysts. In this study, the prevalence of T. gondii in 313 house sparrows in Lanzhou, northwestern China was assayed by the modified agglutination test (MAT). Antibodies to T. gondii were positive in 39 (12.46%) of 313 samples (MAT titer ≥ 1:5). Tissues of heart, brain, and lung from the 39 seropositive house sparrows were tested for T. gondii DNA, 11 of which were found to be positive for the T. gondii B1 gene by PCR amplification. These positive DNA samples were typed at 9 genetic markers, including 8 nuclear loci, i.e., SAG1, 5'- and 3'-SAG2, alternative SAG2, SAG3, GRA6, L358, PK1, c22-8 and an apicoplast locus Apico. Of them, 4 isolates were genotyped with complete data for all loci, and 2 genotypes (Type II variants; ToxoDB #3 and a new genotype) were identified. These results showed that there is a potential risk for human infection with T. gondii in this region. To our knowledge, this is the first report of T. gondii seroprevalence in house sparrows in China.

Citations

Citations to this article as recorded by

Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis
Chao Chen, Si-Yuan Qin, Xing Yang, Xiao-Man Li, Yanan Cai, Cong-Cong Lei, Quan Zhao, Hany M. Elsheikha, Hongwei Cao
Preventive Veterinary Medicine.2024; 226: 106187. CrossRef
Molecular detection of Toxoplasma gondii (Chromista: Apicomplexa) in the blood of passerines (Aves: Passeriformes) in south-eastern Armenia
Sargis A. Aghayan, Manan Asikyan, Marko Raković, Daliborka Stanković, Igor V. Fadeev, Hasmik Gevorgyan, Oleg Shcherbakov, Marine Arakelyan, Karen Aghababyan, Abdol Sattar Pagheh, Mehdi Sharif, Ahmad Daryani
Zoologia (Curitiba).2024;[Epub] CrossRef
Two viable Toxoplasma gondii isolates from red-necked wallaby (Macropus rufogriseus) and red kangaroo (M. rufus)
Liulu Yang, Shilin Xin, Niuping Zhu, Junbao Li, Chunlei Su, Yurong Yang
Parasitology International.2023; 92: 102687. CrossRef
Epidemiology and isolation of viable Toxoplasma gondii strain from macropods
Liulu Yang, Hongjie Ren, Niuping Zhu, Gaohui Mao, Junbao Li, Chunlei Su, Yibao Jiang, Yurong Yang
Heliyon.2023; 9(3): e13960. CrossRef
Genetic diversity of Toxoplasma gondii isolates from birds in the world: A systematic review
Tahereh Mikaeili Galeh, Shahabeddin Sarvi, Alireza Khalilian, Seyed Abdollah Hosseini, Ahmad Daryani
Experimental Parasitology.2023; 248: 108480. CrossRef
Metagenomic insights into the composition and function of the gut microbiota of mice infected with Toxoplasma gondii
Jin-Xin Meng, Xin-Yu Wei, Huanping Guo, Yu Chen, Wei Wang, Hong-Li Geng, Xing Yang, Jiang Jiang, Xiao-Xuan Zhang
Frontiers in Immunology.2023;[Epub] CrossRef
Low Prevalence of Toxoplasma gondii in Sheep and Isolation of a Viable Strain from Edible Mutton from Central China
Yibao Jiang, Shilin Xin, Yiheng Ma, Heng Zhang, Xu Yang, Yurong Yang
Pathogens.2023; 12(6): 827. CrossRef
Abundance of Non-Native Birds in the City: Spatial Variation and Relationship with Socioeconomics in a South American City
Macarena Silva-Ortega, Catalina B. Muñoz-Pacheco, Nélida R. Villaseñor
Animals.2023; 13(11): 1737. CrossRef
Investigation of Toxoplasma gondii in wastewater and surface water in the Qinghai-Tibet Plateau, China using real-time PCR and multilocus genotyping
Anna Lass, Ioannis Kontogeorgos, Liqing Ma, Xueyong Zhang, Xiuping Li, Panagiotis Karanis
Scientific Reports.2022;[Epub] CrossRef
Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020
J. P. Dubey, F. H. A. Murata, C. K. Cerqueira-Cézar, O. C. H. Kwok, C. Su
Parasitology.2021; 148(1): 1. CrossRef
Molecular Prevalence and Genetic Characterization of Toxoplasma gondii in Wild Birds in Hunan Province, China
Meng-Ting Liu, Wei-Xing Jiang, Bin-Ze Gui, Yuan-Chun Jin, Jia-Ning Yi, Fen Li, Wen-Bin Zheng, Guo-Hua Liu
Vector-Borne and Zoonotic Diseases.2019; 19(5): 378. CrossRef
Seroprevalence and risk assessment of Toxoplasma gondii in Java sparrows (Lonchura oryzivora) in China
Si-Yang Huang, Yi-Min Fan, Kai Chen, Qiu-Xia Yao, Bin Yang
BMC Veterinary Research.2019;[Epub] CrossRef
Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China
Zhao-feng Hou, Shi-jie Su, Dan-dan Liu, Le-le Wang, Chuan-li Jia, Zhen-xing Zhao, Yi-fei Ma, Qiao-qiao Li, Jin-jun Xu, Jian-ping Tao
Infection, Genetics and Evolution.2018; 60: 17. CrossRef
First genetic characterization of Toxoplasma gondii infection in common quails (Coturnix coturnix) intended for human consumption in China
Wei Cong, Hong-Liang Ju, Xiao-Xuan Zhang, Qing-Feng Meng, Jian-Gang Ma, Ai-Dong Qian, Xing-Quan Zhu
Infection, Genetics and Evolution.2017; 49: 14. CrossRef
Prevalence and genetic characterization of Toxoplasma gondii in badgers ( Melogale moschata ) in southern China by PCR-RFLP
Kai Chen, Si-Yang Huang, Jin-Lei Wang, Rong-Liang Hu, Qiu-Xia Yao, Shou-Feng Zhang, Xing-Quan Zhu, Quan Liu
Infection, Genetics and Evolution.2017; 52: 30. CrossRef
Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
Infection, Genetics and Evolution.2017; 53: 227. CrossRef
Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview
Ming Pan, Congcong Lyu, Junlong Zhao, Bang Shen
Frontiers in Microbiology.2017;[Epub] CrossRef
Molecular detection of Toxoplasma gondii in house sparrow (Passer domesticus) by LAMP and PCR methods in Tehran, Iran
Amir Abdoli, Abdolhossein Dalimi, Haleh Soltanghoraee, Fatemeh Ghaffarifar
Journal of Parasitic Diseases.2016; 40(4): 1317. CrossRef
Seroprevalence and genotypes of Toxoplasma gondii isolated from pigs intended for human consumption in Liaoning province, northeastern China
Dawei Wang, Yan Liu, Tiantian Jiang, Guoxin Zhang, Gaoming Yuan, Jianbin He, Chunlei Su, Na Yang
Parasites & Vectors.2016;[Epub] CrossRef
Multi-scale occupancy approach to estimate Toxoplasma gondii prevalence and detection probability in tissues: an application and guide for field sampling
Stacey A. Elmore, Kathryn P. Huyvaert, Larissa L. Bailey, Asma Iqbal, Chunlei Su, Brent R. Dixon, Ray T. Alisauskas, Alvin A. Gajadhar, Emily J. Jenkins
International Journal for Parasitology.2016; 46(9): 563. CrossRef
Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP
Wen-Bin Zheng, Xiao-Xuan Zhang, Jian-Gang Ma, Fa-Cai Li, Quan Zhao, Si-Yang Huang, Xing-Quan Zhu, Gordon Langsley
PLOS ONE.2016; 11(11): e0165308. CrossRef
Toxoplasma gondii infection in cancer patients: Prevalence, risk factors, genotypes and association with clinical diagnosis
Wei Cong, Guo-Hua Liu, Qing-Feng Meng, Wei Dong, Si-Yuan Qin, Fu-Kai Zhang, Xiang-Yan Zhang, Xiang-Yang Wang, Ai-Dong Qian, Xing-Quan Zhu
Cancer Letters.2015; 359(2): 307. CrossRef
Prevalence of Toxoplasma gondii in Dogs in Zhanjiang, Southern China
Hai-Hai Jiang, Ming-Wei Li, Min-Jun Xu, Wei Cong, Xing-Quan Zhu
The Korean Journal of Parasitology.2015; 53(4): 493. CrossRef
Prevalence of Antibody toToxoplasma gondiiin Black-headed Gulls (Chroicocephalus ridibundus), Dianchi Lake, China
Qiang Miao, Jiang-qiang Han, Xun Xiang, Fei-Zhou Yuan, Yong-zhang Liu, Gang Duan, Xing-quan Zhu, Feng-cai Zou
Journal of Wildlife Diseases.2014; 50(3): 717. CrossRef
Seroprevalence and genetic characterization of Toxoplasma gondii in three species of pet birds in China
Wei Cong, Qing-Feng Meng, Hui-Qun Song, Dong-Hui Zhou, Si-Yang Huang, Ai-Dong Qian, Chunlei Su, Xing-Quan Zhu
Parasites & Vectors.2014;[Epub] CrossRef
First Report of Genotyping ofToxoplasma gondiiin Free-LivingMicrotus fortisin Northeastern China
Xiao-Xuan Zhang, Si-Yang Huang, Ying-Guang Zhang, Yuan Zhang, Xing-Quan Zhu, Quan Liu
Journal of Parasitology.2014; 100(5): 692. CrossRef
Genetic characterization of Toxoplasma gondii from cats in Yunnan Province, Southwestern China
Yi-Ming Tian, Si-Yang Huang, Qiang Miao, Hai-Hai Jiang, Jian-Fa Yang, Chunlei Su, Xing-Quan Zhu, Feng-Cai Zou
Parasites & Vectors.2014;[Epub] CrossRef
Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP
Hai-Hai Jiang, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Chunlei Su, Shun-Zhou Deng, Xing-Quan Zhu
Parasites & Vectors.2013;[Epub] CrossRef

11,273 View
93 Download
Crossref

Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea: Hye-Youn Kim, Yun-Ah Kim, Ho Sa Lee, Ho Gun Rhie, Shin-Hyeong Cho, Jae-Ran Yu, Sang-Eun Lee; Korean J Parasitol 2009;47(4):413-415.
Published online December 2, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.413

Abstract
PDF

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5'and 3'ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.

Citations

Citations to this article as recorded by

An outbreak of toxoplasmosis in squirrel monkeys (Saimiri sciureus) in South Korea
Hanseul Oh, Kyung‐Yeon Eo, Sanjeev Gumber, Jung Joo Hong, C‐Yoon Kim, Hyun‐Ho Lee, Young‐Mok Jung, Jin Kim, Gyu‐Whan Whang, Ji‐Min Lee, Yong‐Gu Yeo, Bokyeong Ryu, Ji‐Sook Ryu, Seul‐Kee Lee, Ukjin Kim, Sin‐Geun Kang, Jae‐Hak Park
Journal of Medical Primatology.2018; 47(4): 238. CrossRef
Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
Infection, Genetics and Evolution.2017; 53: 227. CrossRef
A high seroprevalence ofToxoplasma gondiiantibodies in a population of feral cats in the Western Cape province of South Africa
Kenneth Hammond-Aryee, Monika Esser, Lesley van Helden, Paul van Helden
Southern African Journal of Infectious Diseases.2015; 30(4): 141. CrossRef
Prevalence and genetic characterization of Toxoplasma gondii infection in bats in southern China
H.H. Jiang, S.Y. Qin, W. Wang, B. He, T.S. Hu, J.M. Wu, Q.S. Fan, C.C. Tu, Q. Liu, X.Q. Zhu
Veterinary Parasitology.2014; 203(3-4): 318. CrossRef
Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea
Sung-Hee Hong, Sang-Eun Lee, Young-Il Jeong, Heung-Chul Kim, Sung-Tae Chong, Terry A. Klein, Jin-Won Song, Se Hun Gu, Shin-Hyeong Cho, Won-Ja Lee
Veterinary Parasitology.2014; 205(3-4): 512. CrossRef
Prevalence and Genetic Characterization of Toxoplasma gondii in Bats in Myanmar
Hongchao Sun, Yiyin Wang, Yingguang Zhang, Wei Ge, Fuqiang Zhang, Biao He, Zuosheng Li, Quanshui Fan, Wei Wang, Changchun Tu, Jiping Li, Quan Liu
Applied and Environmental Microbiology.2013; 79(11): 3526. CrossRef

11,408 View
86 Download
Crossref

Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia: Juri Kim, Myeong Heon Shin, Kyoung-Ju Song, Soon-Jung Park; Korean J Parasitol 2009;47(3):287-291.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.287

Abstract
PDF

The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant α-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of α-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia.

Citations

Citations to this article as recorded by

Genetic diversity and molecular diagnosis of Giardia
Yankai Chang, Junqiang Li, Longxian Zhang
Infection, Genetics and Evolution.2023; 113: 105482. CrossRef
Tubulin as a potential molecular target for resveratrol in Giardia lamblia trophozoites, in vitro and in silico approaches
José Roberto Vargas-Villanueva, Filiberto Gutiérrez-Gutiérrez, Mariana Garza-Ontiveros, Sendar Daniel Nery-Flores, Lizeth Guadalupe Campos-Múzquiz, Dagoberto Vazquez-Obregón, Raul Rodriguez-Herrera, Lissethe Palomo-Ligas
Acta Tropica.2023; 248: 107026. CrossRef
Phosphorylation of Serine 148 in Giardia lamblia End‐binding 1 Protein is Important for Cell Division
Juri Kim, Hye‐Yeon Lee, Kyu‐Ho Lee, Soon‐Jung Park
Journal of Eukaryotic Microbiology.2017; 64(4): 464. CrossRef
Host defences against Giardia lamblia
G. Lopez‐Romero, J. Quintero, H. Astiazarán‐García, C. Velazquez
Parasite Immunology.2015; 37(8): 394. CrossRef
Characterization of Microtubule-Binding and Dimerization Activity of Giardia lamblia End-Binding 1 Protein
Juri Kim, Sara Nagami, Kyu-Ho Lee, Soon-Jung Park, Ira J. Blader
PLoS ONE.2014; 9(5): e97850. CrossRef
Zoonotic potential of Giardia
Una Ryan, Simone M. Cacciò
International Journal for Parasitology.2013; 43(12-13): 943. CrossRef
Identification of Antigenic Proteins in Trichomonas vaginalis
Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park
The Korean Journal of Parasitology.2011; 49(1): 79. CrossRef

8,386 View
115 Download
Crossref

Original Articles

Genetic Variability of Antigen B among Echinococcus granulosus Egyptian Isolates: Gihan M. Tawfeek, Hala S. Elwakil, Nabil S. Awad, Laila El-Hoseiny, Hala S. Thabet, Rania M. Sarhan, Samar K. Darweesh, Wagida A. Anwar; Korean J Parasitol 2009;47(3):259-264.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.259

Abstract
PDF

Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.

Citations

Citations to this article as recorded by

Characterization of antigen B subunit 2 (AgB2) gene polymorphism in sheep isolates of Echinococcus granulosus sensu lato and effect on serologic response
Harun Kaya Kesik, Figen Celik, Seyma Gunyakti Kilinc, Muhammet Uslug, Sami Simsek
Journal of Immunological Methods.2025; 542: 113896. CrossRef
Detection of Echinococcus granulosus sensu lato cysts and seroprevalence of cystic echinococcosis in cattle and camels in Maiduguri Abattoir
Rebecca Arin Yakubu, Ishaya Haruna Nock, Iliya Shehu Ndams, Sodangi Abdulkarim Luka, Clement Ameh Yaro, Luay Alkazmi, Gaber El-Saber Batiha
Journal of Parasitic Diseases.2022; 46(3): 876. CrossRef
Genotyping and Identification of Antigen B Gene Polymorphism of Echinococcus granulosus in Edirne, Thrace, and the First Report of Genotype G2 (Tasmanian Sheep Strain) in Turkey
Taner Tarladaçalışır, Canan Eryıldız, Kıymet Tabakçıoğlu, Nermin Şakru
Balkan Medical Journal.2022; 39(6): 401. CrossRef
Determination of Echinococcus granulosus Antigen B Polymorphism in Human and Animal Isolates From Alborz Province by PCR-SSCP
Parisa Asheri, Abolfazl Miahipour, Mohammad Zibaei, Hamid Hosseini
International Journal of Enteric Pathogens.2021; 9(3): 90. CrossRef
Identification of antigen B (AgB) Gene polymorphism in cattle and sheep isolates of Echinococcus granulosus and investigation of effects on serological diagnosis
Harun Kaya Kesik, Sami Simsek, Seyma Gunyakti Kilinc, Ergun Koroglu
Acta Tropica.2019; 199: 105099. CrossRef
Sero-epidemiological value of some hydatid cyst antigen in diagnosis of human cystic echinococcosis
Mohey Abdelhafez Hassanain, Raafat Mohamed Shaapan, Fathia Abdelrazik M. Khalil
Journal of Parasitic Diseases.2016; 40(1): 52. CrossRef
Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen
Yosra H. Alam-Eldin, Heba E. Abdel Aaty, Mona A. Ahmed
Acta Parasitologica.2015;[Epub] CrossRef
Mutation scanning analysis of genetic variation within and among Echinococcus species: Implications and future prospects
Abdul Jabbar, Robin B. Gasser
ELECTROPHORESIS.2013; 34(13): 1852. CrossRef
Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit (12 Kda), assessed by ELISA, in the diagnosis of human cystic echinococcosis
Gihan M. Tawfeek, Hala S. Elwakil, Laila El-hoseiny, Hala S. Thabet, Rania M. Sarhan, Nabil S. Awad, Wagida A. Anwar
Parasitology Research.2011; 108(2): 371. CrossRef

9,497 View
79 Download
Crossref

Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea: Jae-Hwan Park, Sang-Mee Guk, Eun-Taek Han, Eun-Hee Shin, Jae-Lip Kim, Jong-Yil Chai; Korean J Parasitol 2006;44(1):27-33.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.27

Abstract
PDF

Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified Ziehl-Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases constituted 7 (41.2%) of 17 examined, and goat cases 3 (42.9%) of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.

Citations

Citations to this article as recorded by

Unraveling the Cryptosporidium Threat: Epidemiology, Genetic Diversity, and Prevalence in HIV-Positive Patients in Tehran, Iran
Saghi Rashidifar, Mohammad Javad Gharavi, Naser Harzandi, Zohreh Momeni, Malihe Hasan Nezhad
Diagnostic Microbiology and Infectious Disease.2025; 113(2): 116918. CrossRef
Cryptosporidium in cattle: Assessing the zoonotic risk
Sugandika Bulumulla, Lihua Xiao, Yaoyu Feng, Amanda Ash, Joshua Aleri, Una Ryan, Amanda D. Barbosa
Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100279. CrossRef
Molecular Detection and Genotyping of Enterocytozoon bieneusi in Environmental Sources near Cattle Farms in Korea
Haeseung Lee, Myungji Jo, Hyeyeon Kim, Kaifa Nazim, Seung-Hun Lee, Min-Goo Seo, Sang-Joon Park, Man Hee Rhee, Dongmi Kwak
International Journal of Molecular Sciences.2025; 26(15): 7270. CrossRef
Food and Waterborne Cryptosporidiosis from a One Health Perspective: A Comprehensive Review
Munwar Ali, Yaru Ji, Chang Xu, Qazal Hina, Usama Javed, Kun Li
Animals.2024; 14(22): 3287. CrossRef
Outbreak of severe diarrhea due to zoonotic Cryptosporidium parvum and C. xiaoi in goat kids in Chungcheongbuk-do, Korea
Ah-Young Kim, Badriah Alkathiri, Subin Lee, Kyung-Duk Min, Soochong Kim, Sang-Myeong Lee, Wan-Kyu Lee, Dongmi Kwak, Seung-Hun Lee
Parasitology Research.2023; 122(9): 2045. CrossRef
An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans
Una Ryan, Alireza Zahedi, Yaoyu Feng, Lihua Xiao
Animals.2021; 11(11): 3307. CrossRef
Comparative evaluation of Cryptosporidium infection in malnourished and well-nourished children: Parasitic infections are affected by the interaction of nutritional status and socio-demographic characteristics
Solmaz Madadi, Mahmoud Mahami-Oskouei, Mandana Rafeey, Adel Spotin, Nayyereh Aminisani, Leyla Mahami-Oskouei, Roghayeh Ghoyounchi, Reza Berahmat
Comparative Immunology, Microbiology and Infectious Diseases.2020; 68: 101406. CrossRef
Cryptosporidium hominis infections in non-human animal species: revisiting the concept of host specificity
Giovanni Widmer, Pamela C. Köster, David Carmena
International Journal for Parasitology.2020; 50(4): 253. CrossRef
Cryptosporidium species and subtypes in animals inhabiting drinking water catchments in three states across Australia
Alireza Zahedi, Paul Monis, Alexander W. Gofton, Charlotte L. Oskam, Andrew Ball, Andrew Bath, Michael Bartkow, Ian Robertson, Una Ryan
Water Research.2018; 134: 327. CrossRef
Distribution of Giardia duodenalis (Assemblages A and B) and Cryptosporidium parvum amongst migrant workers in Peninsular Malaysia
Norhidayu Sahimin, Benacer Douadi, Ai Lian Yvonne Lim, Jerzy M. Behnke, Siti Nursheena Mohd Zain
Acta Tropica.2018; 182: 178. CrossRef
Common occurrence of Cryptosporidium hominis in asymptomatic and symptomatic calves in France
Romy Razakandrainibe, El Hadji Ibrahima Diawara, Damien Costa, Laetitia Le Goff, Denis Lemeteil, Jean Jacques Ballet, Gilles Gargala, Loïc Favennec, Christine A. Petersen
PLOS Neglected Tropical Diseases.2018; 12(3): e0006355. CrossRef
Cryptosporidium infection in children with cancer undergoing chemotherapy: how important is the prevention of opportunistic parasitic infections in patients with malignancies?
Reza Berahmat, Mahmoud Mahami-Oskouei, Azim Rezamand, Adel Spotin, Nayyereh Aminisani, Roghayeh Ghoyounchi, Solmaz Madadi
Parasitology Research.2017; 116(9): 2507. CrossRef
A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species
SARAH THOMSON, ELISABETH A. INNES, NICHOLAS N. JONSSON, FRANK KATZER
Parasitology Open.2016;[Epub] CrossRef
Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea
Seung-Hun Lee, Dorene VanBik, Ha-Young Kim, Yu-Ran Lee, Jong Wan Kim, Myeongju Chae, Sang-Ik Oh, Youn-Kyoung Goo, Oh-Deog Kwon, Dongmi Kwak
Veterinary Parasitology.2016; 229: 81. CrossRef
Zoonotic Cryptosporidium Species in Animals Inhabiting Sydney Water Catchments
Alireza Zahedi, Paul Monis, Sarah Aucote, Brendon King, Andrea Paparini, Fuchun Jian, Rongchang Yang, Charlotte Oskam, Andrew Ball, Ian Robertson, Una Ryan, Michael E. Grigg
PLOS ONE.2016; 11(12): e0168169. CrossRef
Detection and Molecular Characterization of Cryptosporidium spp. from Wild Rodents and Insectivores in South Korea
Juha Song, C-Yoon Kim, Seo-Na Chang, Tamer Said Abdelkader, Juhee Han, Tae-Hyun Kim, Hanseul Oh, Ji Min Lee, Dong-Su Kim, Jong-Taek Kim, Hong-Shik Oh, Moonsuk Hur, Jae-Hwa Suh, Jae-Hak Park
The Korean Journal of Parasitology.2015; 53(6): 737. CrossRef
Prevalence, molecular characterization and zoonotic potential of Cryptosporidium spp. in goats in Henan and Chongqing, China
Rongjun Wang, Guoquan Li, Bin Cui, Jianying Huang, Zhaohui Cui, Sumei Zhang, Haiju Dong, Daoyou Yue, Longxian Zhang, Changshen Ning, Ming Wang
Experimental Parasitology.2014; 142: 11. CrossRef
Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Semie Hong, Kyungjin Kim, Sejoung Yoon, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu
Journal of Korean Medical Science.2014; 29(10): 1367. CrossRef
Molecular Characterization of Cryptosporidium spp. Isolated From Immunocompromised Patients and Children
Abdollah Rafiei, Zahra Rashno, Alireza Samarbafzadeh, Shahram Khademvatan
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef
Prevalence and Molecular Characterization of Cryptosporidium in Goats across Four Provincial Level Areas in China
Rongsheng Mi, Xiaojuan Wang, Yan Huang, Peng Zhou, Yuxuan Liu, Yongjun Chen, Jun Chen, Wei Zhu, Zhaoguo Chen, Yung-Fu Chang
PLoS ONE.2014; 9(10): e111164. CrossRef
Cryptosporidiumspecies in humans and animals: current understanding and research needs
UNA RYAN, RONALD FAYER, LIHUA XIAO
Parasitology.2014; 141(13): 1667. CrossRef
Epidemiological Characteristics of the First Water-Borne Outbreak of Cryptosporidiosis in Seoul, Korea
Shinje Moon, Wooseok Kwak, Sangwon Lee, Won Kim, Jaeyeon Oh, Seung-Ki Youn
Journal of Korean Medical Science.2013; 28(7): 983. CrossRef
Health Risk Assessment of Cryptosporidium in Tap Water in Korea
Mok-Young Lee, Sang-Jung Park, Eun-Joo Cho, Su-Jeong Park, Sun-Hee Han, Oh-Sang Kwon
Korean Journal of Environmental Health Sciences.2013; 39(1): 32. CrossRef
Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Hyeng-Il Cheun, Kyungjin Kim, Sejoung Yoon, Won-Ja Lee, Woo-Yoon Park, Seobo Sim, Jae-Ran Yu
The Korean Journal of Parasitology.2013; 51(3): 353. CrossRef
Identification and characterization of a Chinese isolate of Cryptosporidium serpentis from dairy cattle
Fu Chen, Huiling Qiu
Parasitology Research.2012; 111(4): 1785. CrossRef
Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples
Camila G. Homem, Alex A. Nakamura, Deuvânia C. Silva, Weslen F. P. Teixeira, Willian M. D. Coelho, Marcelo V. Meireles
Parasitology Research.2012; 110(5): 1741. CrossRef
Genetic characterisation of Cryptosporidium and Giardia from dairy calves: Discovery of species/genotypes consistent with those found in humans
Harshanie Abeywardena, Aaron R. Jex, Matthew J. Nolan, Shane R. Haydon, Melita A. Stevens, Robin W. McAnulty, Robin B. Gasser
Infection, Genetics and Evolution.2012; 12(8): 1984. CrossRef
Molecular epidemiology of cryptosporidiosis: An update
Lihua Xiao
Experimental Parasitology.2010; 124(1): 80. CrossRef
Parasitic Diseases of Companion Animals
Sung Shik Shin
Hanyang Medical Reviews.2010; 30(3): 246. CrossRef
Taxonomy and species delimitation in Cryptosporidium
Ronald Fayer
Experimental Parasitology.2010; 124(1): 90. CrossRef
Risk factors associated with the occurrence of Cryptosporidium parvum infection in calves
A.J Almeida, F.C.R Oliveira, V.M.Q Flores, C.W.G Lopes
Arquivo Brasileiro de Medicina Veterinária e Zootecnia.2010; 62(6): 1325. CrossRef
A multiplex polymerase chain reaction assay to simultaneously distinguish Cryptosporidium species of veterinary and public health concern in cattle
Mónica Santín, Dante S. Zarlenga
Veterinary Parasitology.2009; 166(1-2): 32. CrossRef
Trends in parasitic diseases in the Republic of Korea
Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
Trends in Parasitology.2008; 24(3): 143. CrossRef
Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran
Majid Pirestani, Javid Sadraei, Abdolhossein Dalimi asl, Mahdi Zavvar, Hossein Vaeznia
Parasitology Research.2008; 103(2): 467. CrossRef
Detection of Assemblage A, Giardia duodenalis and Eimeria spp. in alpacas on two Maryland farms
James M. Trout, Mónica Santín, Ronald Fayer
Veterinary Parasitology.2008; 153(3-4): 203. CrossRef
A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age
Mónica Santín, James M. Trout, Ronald Fayer
Veterinary Parasitology.2008; 155(1-2): 15. CrossRef
The use of a nested PCR–RFLP technique, based on the parasite's 18S ribosomal RNA, to characteriseCryptosporidiumisolates from HIV/AIDS patients
M. Zavvar, J. Sadraei, H. Emadi, M. Pirestani
Annals of Tropical Medicine & Parasitology.2008; 102(7): 597. CrossRef
Emerging Parasitic Diseases in Korea
Jong-Yil Chai
Journal of the Korean Medical Association.2007; 50(11): 946. CrossRef
A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea
Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai
The Korean Journal of Parasitology.2006; 44(4): 367. CrossRef

10,416 View
88 Download
Crossref

Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii: Aifen Lin, Eun-Hee Shin, Tae-Yun Kim, Jae-Hwan Park, Sang-Mee Guk, Jong-Yil Chai; Korean J Parasitol 2005;43(1):27-32.
Published online March 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.1.27

Abstract
PDF

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.

Citations

Citations to this article as recorded by

Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
Infection, Genetics and Evolution.2017; 53: 227. CrossRef
Toxoplasma gondii B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai
The Korean Journal of Parasitology.2015; 53(3): 259. CrossRef
Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis
Shantaveer Biradar, Buddhi Saravanan, Anup Tewari, Chirukandoth Sreekumar, Muthu Sankar, Naduvanahalli Sudhakar
Acta Parasitologica.2014;[Epub] CrossRef
Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6
Ji Su Ma, Miwa Sasai, Jun Ohshima, Youngae Lee, Hironori Bando, Kiyoshi Takeda, Masahiro Yamamoto
Journal of Experimental Medicine.2014; 211(10): 2013. CrossRef
Immune Responses of Mice Intraduodenally Infected withToxoplasma gondiiKI-1 Tachyzoites
Eun-Hee Shin, Yeoun Sook Chun, Won-Hee Kim, Jae-Lip Kim, Kyoung-Ho Pyo, Jong-Yil Chai
The Korean Journal of Parasitology.2011; 49(2): 115. CrossRef
Proteomic Analysis ofToxoplasma gondiiKI-1 Tachyzoites
Si-Hwan Choi, Tae Yun Kim, Sung Goo Park, Guang-Ho Cha, Dae-Whan Shin, Jong-Yil Chai, Young-Ha Lee
The Korean Journal of Parasitology.2010; 48(3): 195. CrossRef
Suppression of CD4+T-Cells in the Spleen of Mice Infected withToxoplasma gondiiKI-1 Tachyzoites
Won-Hee Kim, Eun-Hee Shin, Jae-Lip Kim, Seung-Young Yu, Bong-Kwang Jung, Jong-Yil Chai
The Korean Journal of Parasitology.2010; 48(4): 325. CrossRef
Selection of Polymorphic Peptides from GRA6 and GRA7 Sequences of Toxoplasma gondii Strains To Be Used in Serotyping
Susana Sousa, Daniel Ajzenberg, Maggy Marle, Dominique Aubert, Isabelle Villena, José Correia da Costa, Marie-Laure Dardé
Clinical and Vaccine Immunology.2009; 16(8): 1158. CrossRef
Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis
Juan-Hua Quan, Tae Yun Kim, In-Uk Choi, Young-Ha Lee
The Korean Journal of Parasitology.2008; 46(2): 105. CrossRef
Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis
Eun-Hee Shin, Dong-Hee Kim, Aifen Lin, Jo-Woon-Yi Lee, Hyo-Jin Kim, Myoung-Hee Ahn, Jong-Yil Chai
The Korean Journal of Parasitology.2008; 46(1): 45. CrossRef
Trends in parasitic diseases in the Republic of Korea
Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
Trends in Parasitology.2008; 24(3): 143. CrossRef
Use of GRA6-Derived Synthetic Polymorphic Peptides in an Immunoenzymatic Assay To Serotype Toxoplasma gondii in Human Serum Samples Collected from Three Continents
Susana Sousa, Daniel Ajzenberg, Manuel Vilanova, José Costa, Marie-Laure Dardé
Clinical and Vaccine Immunology.2008; 15(9): 1380. CrossRef

8,779 View
71 Download
Crossref

Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene: Hak-Sun Yu, Mee-Yul Hwang, Tae-Ook Kim, Ho-Cheol Yun, Tae-Ho Kim, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 1999;37(3):181-188.
Published online September 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.3.181

Abstract
PDF

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close interrelationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18S rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but the divergence between them was less than in 18S riboprinting. Acanthamoeba griffini of morphological group 2 branched between pathogenic (A. culbertsoni A-1 and A. healyi OC-3A) and nonpathogenic (A. palestinensis Reich, A. pustulosa GE-3a, A. royreba Oak Ridge, and A lenticulata PD2S) strains of morphological group 3. Among the remaining isolates of morphological group 2, the Chang strain had the identical mitochondrial riboprints as the type strain of A. hatchetti. AA2 and AA1, the type strains of A. divionensis and A. paradivionensis, respectively, had the identical riboprints as A. quina Vil3 and A. castellanii Ma. Although the branching orders of A. castellanii Neff, A. polyphaga P23, A. triangularis SH621, and A. lugdunensis L3a were different from those in 18S riboprinting analysis, the results obtained from this study generally coincided well with those from 18S riboprinting. Mitochondrial riboprinting may have an advantage over nuclear 18S rDNA riboprinting because the mt SSU rDNAs do not seem to have introns that are found in the 18S genes of Acanthamoeba and that distort phylogenetic analyses.

Citations

Citations to this article as recorded by

Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?
Merve Kahraman, Zübeyda Akın Polat
Turkish Journal of Parasitology.2024; 48(1): 15. CrossRef
The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Paul A. Fuerst
Microorganisms.2023; 11(2): 295. CrossRef
Acanthamoeba Mannose and Laminin Binding Proteins Variation across Species and Genotypes
Daniele Corsaro
Microorganisms.2022; 10(11): 2162. CrossRef
DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia
Inese Gavarāne, Jūlija Trofimova, Artjoms Mališevs, Olga Valciņa, Muza Kirjušina, Ilze Rubeniņa, Aivars Bērziņš
Parasitology Research.2018; 117(10): 3299. CrossRef
Genotyping of Acanthamoeba T15: the environmental strain in Turkey
G. Evyapan, I. S. Koltas, F. Eroglu
Transactions of the Royal Society of Tropical Medicine and Hygiene.2015; 109(3): 221. CrossRef
Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA
Md Moshiur Rahman, Kenji Yagita, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
The Korean Journal of Parasitology.2013; 51(4): 401. CrossRef
Molecular Phylogeny of Acanthamoeba
Hyun Hee Kong
The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates
Ying-Hua Xuan, Byung-Suk Chung, Yeon-Chul Hong, Hyun-Hee Kong, Tae-Won Hahn, Dong-Il Chung
The Korean Journal of Parasitology.2008; 46(3): 157. CrossRef
Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2005; 43(1): 7. CrossRef
Pathogenic free-living amoebae in Korea
Ho-Joon Shin, Kyung-il Im
The Korean Journal of Parasitology.2004; 42(3): 93. CrossRef
A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level
Hyun-Hee Kong, Dong-Il Chung
The Korean Journal of Parasitology.2002; 40(1): 25. CrossRef
Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge
Jill M. Schroeder, Gregory C. Booton, John Hay, Ingrid A. Niszl, David V. Seal, Miles B. Markus, Paul A. Fuerst, Thomas J. Byers
Journal of Clinical Microbiology.2001; 39(5): 1903. CrossRef
Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp
Julia Walochnik, Andreas Obwaller, Horst Aspöck
Applied and Environmental Microbiology.2000; 66(10): 4408. CrossRef